Key for the identification of third instar larvae of African blowflies (Diptera Calliphoridae) of forensic importance in death investigations
- Szpila, Krzysztof, Williams, Kirstin A, Soszyńska, Agnieszka, Ekanem, Mfon, Heyns, Marise, Dinka, Mergi D, Villet, Martin H
- Authors: Szpila, Krzysztof , Williams, Kirstin A , Soszyńska, Agnieszka , Ekanem, Mfon , Heyns, Marise , Dinka, Mergi D , Villet, Martin H
- Date: 2022
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/440761 , vital:73810 , https://doi.org/10.1016/j.forsciint.2023.111889
- Description: Blowfly larvae are the insects primarily responsible for the active stage of decomposition of exposed vertebrate remains and are the most frequently collected entomological evidence during forensic investigations of death. The necrophagous calliphorids in continental Africa that consistently develop on large vertebrate carrion include 11 species belonging to four genera: Calliphora, Chrysomya, Hemipyrellia and Lucilia. Most of these species are widespread in Africa and frequently reported on large animal carcasses and carrion and human corpses. A few keys have been compiled for identification of their third instar larvae, but none of them covers the complete set of taxa. Therefore, we provide a new comprehensive key with original illustrations of all taxonomically significant characters. The key is based on characters that should be easily observable even in poorly equipped local laboratories and is a reliable taxonomic tool for material collected in either urban or rural areas where synanthropic species predominate. However, it should be used with some caution in areas with relatively pristine natural habitats, where additional carrion-breeding species may occur. The publication of the key will significantly facilitate both medical and forensic entomological research and practice in Africa.
- Full Text:
- Date Issued: 2022
- Authors: Szpila, Krzysztof , Williams, Kirstin A , Soszyńska, Agnieszka , Ekanem, Mfon , Heyns, Marise , Dinka, Mergi D , Villet, Martin H
- Date: 2022
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/440761 , vital:73810 , https://doi.org/10.1016/j.forsciint.2023.111889
- Description: Blowfly larvae are the insects primarily responsible for the active stage of decomposition of exposed vertebrate remains and are the most frequently collected entomological evidence during forensic investigations of death. The necrophagous calliphorids in continental Africa that consistently develop on large vertebrate carrion include 11 species belonging to four genera: Calliphora, Chrysomya, Hemipyrellia and Lucilia. Most of these species are widespread in Africa and frequently reported on large animal carcasses and carrion and human corpses. A few keys have been compiled for identification of their third instar larvae, but none of them covers the complete set of taxa. Therefore, we provide a new comprehensive key with original illustrations of all taxonomically significant characters. The key is based on characters that should be easily observable even in poorly equipped local laboratories and is a reliable taxonomic tool for material collected in either urban or rural areas where synanthropic species predominate. However, it should be used with some caution in areas with relatively pristine natural habitats, where additional carrion-breeding species may occur. The publication of the key will significantly facilitate both medical and forensic entomological research and practice in Africa.
- Full Text:
- Date Issued: 2022
The first record of Omosita nearctica Kirejtshuk (Coleoptera, Nitidulidae) in South Africa, with the first description of its mature larva
- Williams, Kirstin A, Clitheroe, Crystal-Leigh, Villet, Martin H, Midgley, John M
- Authors: Williams, Kirstin A , Clitheroe, Crystal-Leigh , Villet, Martin H , Midgley, John M
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/440640 , vital:73799 , https://africaninvertebrates.pensoft.net/article/58842/
- Description: Sap beetles of the genus Omosita Erichson are stored-product pests that are also associated with carrion, potentially making them biosecurity risks and forensic tools. The discovery of a specimen of the Nearctic species Omosita nearctica Kirejtshuk in South Africa prompted an investigation a decade later to determine if this species had established itself in the country, which was confirmed by the collection of further breeding specimens that also facilitated the first description of mature larvae of O. nearctica. A new key to adults of all Omosita species is presented.
- Full Text:
- Date Issued: 2021
- Authors: Williams, Kirstin A , Clitheroe, Crystal-Leigh , Villet, Martin H , Midgley, John M
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/440640 , vital:73799 , https://africaninvertebrates.pensoft.net/article/58842/
- Description: Sap beetles of the genus Omosita Erichson are stored-product pests that are also associated with carrion, potentially making them biosecurity risks and forensic tools. The discovery of a specimen of the Nearctic species Omosita nearctica Kirejtshuk in South Africa prompted an investigation a decade later to determine if this species had established itself in the country, which was confirmed by the collection of further breeding specimens that also facilitated the first description of mature larvae of O. nearctica. A new key to adults of all Omosita species is presented.
- Full Text:
- Date Issued: 2021
Morphological identification of Lucilia sericata, Lucilia cuprina and their hybrids (Diptera, Calliphoridae)
- Williams, Kirstin A, Villet, Martin H
- Authors: Williams, Kirstin A , Villet, Martin H
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/441476 , vital:73892 , https://zookeys.pensoft.net/articles.php?id=3908
- Description: Hybrids of Lucilia sericata and Lucilia cuprina have been shown to exist in previous studies using molecular methods, but no study has shown explicitly that these hybrids can be identified morphologically. Published morphological characters used to identify L. sericata and L. cuprina were reviewed, and then scored and tested using specimens of both species and known hybrids. Ordination by multi-dimensional scaling indicated that the species were separable, and that hybrids resembled L. cuprina, whatever their origin. Discriminant function analysis of the characters successfully separated the specimens into three unambiguous groups – L. sericata, L. cuprina and hybrids. The hybrids were morphologically similar irrespective of whether they were from an ancient introgressed lineage or more modern. This is the first evidence that hybrids of these two species can be identified from their morphology. The usefulness of the morphological characters is also discussed and photographs of several characters are included to facilitate their assessment.
- Full Text:
- Date Issued: 2014
- Authors: Williams, Kirstin A , Villet, Martin H
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/441476 , vital:73892 , https://zookeys.pensoft.net/articles.php?id=3908
- Description: Hybrids of Lucilia sericata and Lucilia cuprina have been shown to exist in previous studies using molecular methods, but no study has shown explicitly that these hybrids can be identified morphologically. Published morphological characters used to identify L. sericata and L. cuprina were reviewed, and then scored and tested using specimens of both species and known hybrids. Ordination by multi-dimensional scaling indicated that the species were separable, and that hybrids resembled L. cuprina, whatever their origin. Discriminant function analysis of the characters successfully separated the specimens into three unambiguous groups – L. sericata, L. cuprina and hybrids. The hybrids were morphologically similar irrespective of whether they were from an ancient introgressed lineage or more modern. This is the first evidence that hybrids of these two species can be identified from their morphology. The usefulness of the morphological characters is also discussed and photographs of several characters are included to facilitate their assessment.
- Full Text:
- Date Issued: 2014
Predicting the geographic distribution of Lucilia sericata and Lucilia cuprina (Diptera Calliphoridae) in South Africa
- Williams, Kirstin A, Richards, Cameron S, Villet, Martin H
- Authors: Williams, Kirstin A , Richards, Cameron S , Villet, Martin H
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/440681 , vital:73803 , https://hdl.handle.net/10520/EJC158017
- Description: Lucilia sericata (Meigen, 1826) and Lucilia cuprina (Wiedemann, 1830) (Diptera: Calliphoridae: Luciliinae) have medical, veterinary and forensic importance. Knowing their distribution in South Africa would allow more effective management and utilisation of these flies. Their predicted geographic distributions in South Africa were modelled using maximum entropy analysis of selected climatic variables. The most important environmental variables in modelling the distributions were the magnitude of monthly rainfall and the magnitude of the monthly maximum temperature for L. sericata and the seasonal variation in monthly mean humidity and magnitude of monthly rainfall for L. cuprina. A clear geographical bias was shown in museum records and supports the need for focused surveys. There was no correlation between the predicted distribution of L. cuprina and sheep farming in South Africa, nor between the predicted distribution of L. sericata and human population density. Although their patterns of occurrence differed, both species are widely distributed in South Africa and therefore one cannot identify these flies by locality alone - morphological or molecular identification is necessary.
- Full Text:
- Date Issued: 2014
- Authors: Williams, Kirstin A , Richards, Cameron S , Villet, Martin H
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/440681 , vital:73803 , https://hdl.handle.net/10520/EJC158017
- Description: Lucilia sericata (Meigen, 1826) and Lucilia cuprina (Wiedemann, 1830) (Diptera: Calliphoridae: Luciliinae) have medical, veterinary and forensic importance. Knowing their distribution in South Africa would allow more effective management and utilisation of these flies. Their predicted geographic distributions in South Africa were modelled using maximum entropy analysis of selected climatic variables. The most important environmental variables in modelling the distributions were the magnitude of monthly rainfall and the magnitude of the monthly maximum temperature for L. sericata and the seasonal variation in monthly mean humidity and magnitude of monthly rainfall for L. cuprina. A clear geographical bias was shown in museum records and supports the need for focused surveys. There was no correlation between the predicted distribution of L. cuprina and sheep farming in South Africa, nor between the predicted distribution of L. sericata and human population density. Although their patterns of occurrence differed, both species are widely distributed in South Africa and therefore one cannot identify these flies by locality alone - morphological or molecular identification is necessary.
- Full Text:
- Date Issued: 2014
Ancient and modern hybridization between Lucilia sericata and L. cuprina (Diptera: Calliphoridae)
- Williams, Kirstin A, Villet, Martin H
- Authors: Williams, Kirstin A , Villet, Martin H
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442633 , vital:74017 , 10.14411/eje.2013.029
- Description: There are important but inconsistent differences in breeding site preference between the blow flies Lucilia sericata (Meigen, 1826) and L. cuprina (Wiedemann, 1830)(Diptera: Calliphoridae) that have significance for medical and veterinary science. These inconsistencies might arise from hybridisation. The species are difficult to distinguish using external morphology, although the male genitalia are distinctive and there are reliable molecular markers. Molecular evidence of modern hybridisation, derived from a newly developed nuclear marker, the period (per) gene, is presented here. This has implications for identifications of these species based on mtDNA, and may lead to an explanation of the medical and veterinary anomalies noted in these species.
- Full Text:
- Date Issued: 2013
- Authors: Williams, Kirstin A , Villet, Martin H
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442633 , vital:74017 , 10.14411/eje.2013.029
- Description: There are important but inconsistent differences in breeding site preference between the blow flies Lucilia sericata (Meigen, 1826) and L. cuprina (Wiedemann, 1830)(Diptera: Calliphoridae) that have significance for medical and veterinary science. These inconsistencies might arise from hybridisation. The species are difficult to distinguish using external morphology, although the male genitalia are distinctive and there are reliable molecular markers. Molecular evidence of modern hybridisation, derived from a newly developed nuclear marker, the period (per) gene, is presented here. This has implications for identifications of these species based on mtDNA, and may lead to an explanation of the medical and veterinary anomalies noted in these species.
- Full Text:
- Date Issued: 2013
An accidental but safe and effective use of Lucilia cuprina (Diptera: Calliphoridae) in maggot debridement therapy in Alexandria, Egypt
- Tantawi, Tarek I, Williams, Kirstin A, Villet, Martin H
- Authors: Tantawi, Tarek I , Williams, Kirstin A , Villet, Martin H
- Date: 2010
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442454 , vital:73986 , https://doi.org/10.1093/jmedent/47.3.491
- Description: The calliphorid fly, Lucilia cuprina (Wiedemann), is known to cause serious malign myiasis in animals, whereas its sibling species Lucilia sericata (Meigen) is commonly a carrion breeder and is used in maggot debridement therapy (MDT). The current study reports an accidental involvement of L. cuprina in MDT in Alexandria, Egypt, that has proved to be safe and effective. In November 2008, the laboratory colonies of L. sericata (the species regularly used in MDT) at the Faculty of Science, Alexandria University were renewed by Lucilia flies collected as third instar larvae on exposed rabbit carcasses. Flies from the new colonies were successfully used to heal the diabetic foot wounds of two patients at Alexandria Main University Hospital. Analysis of DNA sequences and adult and larval morphology then revealed that these flies were and still are L. cuprina. Breeding of this species in carrion in Alexandria is a new record. Despite the safety of this strain of L. cuprina in MDT, entomologists rearing blow flies for the purpose of wound debridement should regularly maintain high quality assurance of their species’ identity to avoid possible clinical complications that may result from the introduction of an unexpected and invasive species to their laboratory colonies.
- Full Text:
- Date Issued: 2010
- Authors: Tantawi, Tarek I , Williams, Kirstin A , Villet, Martin H
- Date: 2010
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442454 , vital:73986 , https://doi.org/10.1093/jmedent/47.3.491
- Description: The calliphorid fly, Lucilia cuprina (Wiedemann), is known to cause serious malign myiasis in animals, whereas its sibling species Lucilia sericata (Meigen) is commonly a carrion breeder and is used in maggot debridement therapy (MDT). The current study reports an accidental involvement of L. cuprina in MDT in Alexandria, Egypt, that has proved to be safe and effective. In November 2008, the laboratory colonies of L. sericata (the species regularly used in MDT) at the Faculty of Science, Alexandria University were renewed by Lucilia flies collected as third instar larvae on exposed rabbit carcasses. Flies from the new colonies were successfully used to heal the diabetic foot wounds of two patients at Alexandria Main University Hospital. Analysis of DNA sequences and adult and larval morphology then revealed that these flies were and still are L. cuprina. Breeding of this species in carrion in Alexandria is a new record. Despite the safety of this strain of L. cuprina in MDT, entomologists rearing blow flies for the purpose of wound debridement should regularly maintain high quality assurance of their species’ identity to avoid possible clinical complications that may result from the introduction of an unexpected and invasive species to their laboratory colonies.
- Full Text:
- Date Issued: 2010
A new and earlier record of Chrysomya megacephala in South Africa, with notes on another exotic species, Calliphora vicina (Diptera Calliphoridae)
- Williams, Kirstin A, Villet, Martin H
- Authors: Williams, Kirstin A , Villet, Martin H
- Date: 2006
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/440710 , vital:73805 , https://hdl.handle.net/10520/EJC84554
- Description: South African records of Chrysomya megacephala and Calliphora vicina are reviewed and plotted on maps. Dispersal routes of these two forensically and medically important blowfly species are discussed. Emphasised is the importance of deposition of voucher material in museum collections.
- Full Text:
- Date Issued: 2006
- Authors: Williams, Kirstin A , Villet, Martin H
- Date: 2006
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/440710 , vital:73805 , https://hdl.handle.net/10520/EJC84554
- Description: South African records of Chrysomya megacephala and Calliphora vicina are reviewed and plotted on maps. Dispersal routes of these two forensically and medically important blowfly species are discussed. Emphasised is the importance of deposition of voucher material in museum collections.
- Full Text:
- Date Issued: 2006
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