Contributions to the study of a class of optimal control problems on the orthogonal groups SO(3) and SO(4)
- Authors: Adams, Ross Montague
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64826 , vital:28608
- Description: In this thesis we investigate a class of invariant optimal control problems, and their associated quadratic Hamilton-Poisson systems, on the orthogonal groups SO(3) and SO(4). Specifically, we are concerned with the class of left-invariant control affine systems. We begin by classifying all cost-extended systems on SO(3) under cost equivalence. (Cost-extended systems are closely related to optimal control problems.) A classification of all quadratic Hamilton-Poisson systems on the (minus) Lie-Poisson space so(3)*, under affine equivalence, is also obtained. For the normal forms obtained in our classification (of Hamilton-Poisson systems) we investigate the (Lyapunov) stability nature of the equilibria using spectral and energy-Casimir methods. For a subclass of these systems, we obtain analytic expressions for the integral curves of the associated Hamiltonian vector fields in terms of (basic) Jacobi elliptic functions. The explicit relationship between the classification of cost-extended systems on SO(3) and the classification of quadratic Hamilton- Poisson systems on so(3)* is provided. On SO(4), a classification of all left-invariant control affine systems under L-equivalence is obtained. We then determine which of these representatives are controllable, thus obtaining a classification under detached feedback equivalence. We also obtain a partial classification of quadratic Hamilton-Poisson systems on the Lie-Poisson space so(4)*. An investigation of the stability nature of the equilibria for a subclass of these systems is also done. Several illustrative examples of optimal control problems on the orthogonal group SO(3) are provided. More specifically, we consider an optimal control problem corresponding to a representative of our classification (of cost-extended system) for each possible number of control inputs. For each of these problems, we obtain explicit expressions for the extremal trajectories on the homogeneous space S2 by projecting the extremal trajectories on the group SO(3). The examples provided show how our classifications of cost-extended systems and Hamilton-Poisson systems can be used to obtain the optimal controls and the extremal trajectories corresponding to a large class of optimal control problems on SO(3). An example of a four-input optimal control problem on SO(4) is also provided. This example is provided to show how the solutions of certain problems on SO(4) can be related to the solutions of certain optimal control problems on SO(3).
- Full Text:
- Date Issued: 2015
- Authors: Adams, Ross Montague
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64826 , vital:28608
- Description: In this thesis we investigate a class of invariant optimal control problems, and their associated quadratic Hamilton-Poisson systems, on the orthogonal groups SO(3) and SO(4). Specifically, we are concerned with the class of left-invariant control affine systems. We begin by classifying all cost-extended systems on SO(3) under cost equivalence. (Cost-extended systems are closely related to optimal control problems.) A classification of all quadratic Hamilton-Poisson systems on the (minus) Lie-Poisson space so(3)*, under affine equivalence, is also obtained. For the normal forms obtained in our classification (of Hamilton-Poisson systems) we investigate the (Lyapunov) stability nature of the equilibria using spectral and energy-Casimir methods. For a subclass of these systems, we obtain analytic expressions for the integral curves of the associated Hamiltonian vector fields in terms of (basic) Jacobi elliptic functions. The explicit relationship between the classification of cost-extended systems on SO(3) and the classification of quadratic Hamilton- Poisson systems on so(3)* is provided. On SO(4), a classification of all left-invariant control affine systems under L-equivalence is obtained. We then determine which of these representatives are controllable, thus obtaining a classification under detached feedback equivalence. We also obtain a partial classification of quadratic Hamilton-Poisson systems on the Lie-Poisson space so(4)*. An investigation of the stability nature of the equilibria for a subclass of these systems is also done. Several illustrative examples of optimal control problems on the orthogonal group SO(3) are provided. More specifically, we consider an optimal control problem corresponding to a representative of our classification (of cost-extended system) for each possible number of control inputs. For each of these problems, we obtain explicit expressions for the extremal trajectories on the homogeneous space S2 by projecting the extremal trajectories on the group SO(3). The examples provided show how our classifications of cost-extended systems and Hamilton-Poisson systems can be used to obtain the optimal controls and the extremal trajectories corresponding to a large class of optimal control problems on SO(3). An example of a four-input optimal control problem on SO(4) is also provided. This example is provided to show how the solutions of certain problems on SO(4) can be related to the solutions of certain optimal control problems on SO(3).
- Full Text:
- Date Issued: 2015
Maintenance management systems of on-campus student hostels at Nigerian universities
- Authors: Adamu, Anita Dzikwi
- Date: 2015
- Subjects: School buildings -- Maintenance and repair , Dormitories -- Nigeria , Nigeria -- Student housing
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9710 , http://hdl.handle.net/10948/d1020168
- Description: Most universities have long recognised the importance and contribution of on-campus hostels to the learning process. In so doing, most of these institutions have provided and maintained building facilities for academic purposes (teaching and research). Hostels are integral components of most campuses of Nigerian universities, and they are part of the built assets of the institutions in terms of both administration and maintenance management. Currently, there is little understanding of the maintenance management systems of on-campus hostel buildings in both the public and the private universities. Moreover, there is a dearth of research evidence demonstrating that there is an appreciable difference in the maintenance management systems of the Federal, State and private universities in Nigeria. This research aimed at establishing an understanding of maintenance management systems of on-campus hostel buildings at Nigerian universities owned by the Federal and State governments and the Private Sector, relative to stipulated criteria for best practice.The qualitative method of research inquiry that is rooted in the phenomenological paradigm was employed in the investigations. The research activities included a comprehensive review of the related literature and study of selected cases. The North-central geopolitical zone of Nigeria was the selected geographical scope of this research. Ten universities were purposefully selected for the study, and they include three Federal, four state and three private universities. The nature and differences in the research questions necessitated generating different forms of data. The questions that were related to the maintenance management systems for the hostels in place at the universities were addressed with the outcomes of interviews with the hostel managers. The questions that were about the structures and current conditions of the hostel buildings at the three categories of universities were addressed with data that were generated from condition assessments of the facilities and physical observations with the aid of cameras. The study reveals apparent deteriorated and unhealthy conditions of most of the on-campus hostels at the public universities, while those of the private universities are satisfactory. The research also found that the maintenance management systems in use at all the universities are reactive and lack proper planning and coordination of maintenance activities. There is no significant difference in the maintenance management systems of hostels at the public and the private universities and the systems have major negative impact on the conditions of the buildings and their performances. The research has developed a conceptual model for the study of maintenance management informed by the theoretical framework. The model considered the impact of the external environmental factors on the management and maintenance of the hostels. Secondly, the interrelationships of strategic and performance management with maintenance management in developing an effective maintenance management system were established. The study has achieved its aim of establishing an understanding of maintenance management systems of on-campus hostel buildings at Nigerian universities owned by the Federal and, State governments and the Private Sector. The maintenance management model developed in the study is recommended to maintenance management departments as a guide for effective maintenance of the built facilities in their institutions.
- Full Text:
- Date Issued: 2015
- Authors: Adamu, Anita Dzikwi
- Date: 2015
- Subjects: School buildings -- Maintenance and repair , Dormitories -- Nigeria , Nigeria -- Student housing
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9710 , http://hdl.handle.net/10948/d1020168
- Description: Most universities have long recognised the importance and contribution of on-campus hostels to the learning process. In so doing, most of these institutions have provided and maintained building facilities for academic purposes (teaching and research). Hostels are integral components of most campuses of Nigerian universities, and they are part of the built assets of the institutions in terms of both administration and maintenance management. Currently, there is little understanding of the maintenance management systems of on-campus hostel buildings in both the public and the private universities. Moreover, there is a dearth of research evidence demonstrating that there is an appreciable difference in the maintenance management systems of the Federal, State and private universities in Nigeria. This research aimed at establishing an understanding of maintenance management systems of on-campus hostel buildings at Nigerian universities owned by the Federal and State governments and the Private Sector, relative to stipulated criteria for best practice.The qualitative method of research inquiry that is rooted in the phenomenological paradigm was employed in the investigations. The research activities included a comprehensive review of the related literature and study of selected cases. The North-central geopolitical zone of Nigeria was the selected geographical scope of this research. Ten universities were purposefully selected for the study, and they include three Federal, four state and three private universities. The nature and differences in the research questions necessitated generating different forms of data. The questions that were related to the maintenance management systems for the hostels in place at the universities were addressed with the outcomes of interviews with the hostel managers. The questions that were about the structures and current conditions of the hostel buildings at the three categories of universities were addressed with data that were generated from condition assessments of the facilities and physical observations with the aid of cameras. The study reveals apparent deteriorated and unhealthy conditions of most of the on-campus hostels at the public universities, while those of the private universities are satisfactory. The research also found that the maintenance management systems in use at all the universities are reactive and lack proper planning and coordination of maintenance activities. There is no significant difference in the maintenance management systems of hostels at the public and the private universities and the systems have major negative impact on the conditions of the buildings and their performances. The research has developed a conceptual model for the study of maintenance management informed by the theoretical framework. The model considered the impact of the external environmental factors on the management and maintenance of the hostels. Secondly, the interrelationships of strategic and performance management with maintenance management in developing an effective maintenance management system were established. The study has achieved its aim of establishing an understanding of maintenance management systems of on-campus hostel buildings at Nigerian universities owned by the Federal and, State governments and the Private Sector. The maintenance management model developed in the study is recommended to maintenance management departments as a guide for effective maintenance of the built facilities in their institutions.
- Full Text:
- Date Issued: 2015
A framework for cloud computing adoption in small and medium-sized enterprises : a case of the Accra - Tema metropolis in Ghana
- Authors: Adane, Martin
- Date: 2015
- Subjects: Cloud computing -- Ghana Small business -- Ghana
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/13803 , vital:39713
- Description: Cloud computing adoption and usage is important to achieving business competition. This is done by making it a competitive tool for firms. The adoption of cloud computing enables firms to achieve greater business competency, improve performance, and allows them to maintain their competitive advantage. Since its emergence, there has been a surge in the adoption of cloud computing with research into its adoption primarily concentrated on bigger firms. However, a major characteristic of cloud computing is the anticipated possibilities it holds for small and medium-sized enterprises (SMEs). SMEs typically operate differently from larger firms and are not limited by resource constraints. For SMEs, the reduction in the financial burden normally associated with the adoption of new technologies is a significant benefit of cloud computing due to their financial constraints. In Ghana, SMEs mostly use obsolete technologies and have a slow response towards new technologies. Thus, they are unable to harness the numerous opportunities technology presents to them to stay competitive. Cloud computing is still regarded as a new technology in the business world, therefore research that focuses on its adoption by SMEs to help them stay competitive is minimal. Available research on cloud computing in Ghana does not provide clear guidelines for ensuring a successful adoption process and the continued use of cloud computing services. This study seeks to investigate how a framework can assist SMEs in their use of cloud computing in the Accra-Tema metropolis of Ghana. A knowledge of the factors associated with adoption decisions and those that significantly influence the decision are required to ensure a successful adoption process. The empirical data was gathered using a questionnaire and face-to-face interviews developed from literature and administered to users and potential users of cloud computing. The questionnaire and interviews primarily investigate key adoption factors and the findings are reported in this research study. The findings reveal interesting insights into understanding issues that affect the overall decision to adopt and use cloud computing services by SMEs. The findings show that the adoption of cloud computing can improve information management practices within SMEs. The findings also reveal that several factors need to be considered in the overall decision to adopt and use cloud computing to ensure a successful adoption process. An initial cloud computing adoption model was proposed based on the empirical findings. Key adoption factors of the initial adoption model include adoption benefits and drivers, concerns and barriers, adoption interventions, and information management in the cloud. computing adoption framework. The proposed adoption framework aims to assist SMEs to adopt and use cloud computing services and make them relevant in the global market.
- Full Text:
- Date Issued: 2015
- Authors: Adane, Martin
- Date: 2015
- Subjects: Cloud computing -- Ghana Small business -- Ghana
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/13803 , vital:39713
- Description: Cloud computing adoption and usage is important to achieving business competition. This is done by making it a competitive tool for firms. The adoption of cloud computing enables firms to achieve greater business competency, improve performance, and allows them to maintain their competitive advantage. Since its emergence, there has been a surge in the adoption of cloud computing with research into its adoption primarily concentrated on bigger firms. However, a major characteristic of cloud computing is the anticipated possibilities it holds for small and medium-sized enterprises (SMEs). SMEs typically operate differently from larger firms and are not limited by resource constraints. For SMEs, the reduction in the financial burden normally associated with the adoption of new technologies is a significant benefit of cloud computing due to their financial constraints. In Ghana, SMEs mostly use obsolete technologies and have a slow response towards new technologies. Thus, they are unable to harness the numerous opportunities technology presents to them to stay competitive. Cloud computing is still regarded as a new technology in the business world, therefore research that focuses on its adoption by SMEs to help them stay competitive is minimal. Available research on cloud computing in Ghana does not provide clear guidelines for ensuring a successful adoption process and the continued use of cloud computing services. This study seeks to investigate how a framework can assist SMEs in their use of cloud computing in the Accra-Tema metropolis of Ghana. A knowledge of the factors associated with adoption decisions and those that significantly influence the decision are required to ensure a successful adoption process. The empirical data was gathered using a questionnaire and face-to-face interviews developed from literature and administered to users and potential users of cloud computing. The questionnaire and interviews primarily investigate key adoption factors and the findings are reported in this research study. The findings reveal interesting insights into understanding issues that affect the overall decision to adopt and use cloud computing services by SMEs. The findings show that the adoption of cloud computing can improve information management practices within SMEs. The findings also reveal that several factors need to be considered in the overall decision to adopt and use cloud computing to ensure a successful adoption process. An initial cloud computing adoption model was proposed based on the empirical findings. Key adoption factors of the initial adoption model include adoption benefits and drivers, concerns and barriers, adoption interventions, and information management in the cloud. computing adoption framework. The proposed adoption framework aims to assist SMEs to adopt and use cloud computing services and make them relevant in the global market.
- Full Text:
- Date Issued: 2015
Isolation, characterisation of terpenoids and biosynthesis of silver nanoparticles of acacia mearnsii de wild and acacia Karroo Hayne and their Bioassays
- Authors: Avoseh, Opeyemi Nudewhenu
- Date: 2015
- Language: English
- Type: Thesis , Doctoral , PhD (Chemistry)
- Identifier: vital:11355 , http://hdl.handle.net/10353/d1021292
- Description: Great wealth of traditional knowledge about the use of plants had been transferred from generation to generations leading to the present day drug discovery and invention of new scientific methods of isolation, purification and identification. With the discovery of new diseases and drug-resistant organisms, there is no other source or deposit of lead compounds or drugs than the plant kingdom. As a result of this, about 25% of the current drug administered owe their origin to plant sources with the view to reduce the carcinogenic effect of synthetic drugs. Volatile terpenoids among other broad spectrum of natural product had been implicated to show high therapeutic properly. In the present study, selected locally-used medicinal plants were exploited for the presence of potent bioactive compounds and ability to form nanoparticles with distinctive property for use as chemoprotective agent against inflammation, tumors, cancer and other chronic diseases. Acacia mearnsii De Wild and Acacia karroo Hayne studied in this report are known to be invasive species with no proper regulation to conserve and preserve them. However, ethnopharmacology report of these plant species in the Southern Africa region reveals that they are good antiseptic, anti-diarrhea, anti-inflammation and a forage for livestock. These plants were subjected to volatile extraction protocol of some parts of the plants (stem and leaves) followed by examination of the anti-inflammation capacity of the extracts using an animal model. In addition, the bye-product (hydrosol) from the stem bark of each species possess a high reducing and stabilizing property leading to synthesis of silver nanoparticles, followed by investigation of the anti-inflammation potential of the synthesized silver nanoparticles using animal model. The volatile oils of the leaves and stem bark of Acacia mearnsii De Wild obtained by hydro-distillation were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Twenty, Thirty-Eight, Twenty-nine and Thirty-Eight components accounting for 93.8%, 92.1%, 78.5% and 90.9% of the total oils of the fresh, dry leaves and fresh, dry stem bark respectively. The major components of the oil were octadecyl alcohol (25.5%) and phytol (10.5%); cis-verbenol (29.5%); phytol (10.1%) and phytol (23.4%) for the fresh leaves, dried leaves, and fresh stem, dry stem bark respectively. Oral administration of essential oils at the dose of 2% showed significant (p<0.05) anti-inflammatory properties in the albumin induced test model in rats. Oils from the fresh leaves and dry stems inhibited inflammation beyond 4 h post treatment. Furthermore, the chemical composition of the essential oils obtained by hydro-distillation from the leaves and stem bark (dry and fresh) of Acacia karroo Hayne, analysed by GC-MS, shows that hexanal (10.67%) and ß-ionone (9.74%) were dominant in the dried leaves, β-pinene (14.30%), and (Z)-2-Hexen-1-ol (10.21%) in the fresh leaves while Octacosane (10.59%) and phytol (23.38%) were dominant in the dry and fresh stem respectively. The anti-inflammation ability of these oils after an albumin-induced inflammation on wistar rats, shows a significant effect at the 1st h of treatment with a significance of P< 0.01 for all part plants, while the fresh leaves shows further inhibitory activities at the 2nd h of analysis. Silver nanoparticles (AgNPs) were successfully synthesized from AgNO3 through a green route using the aqueous extract (hydrosols) of Acacia mearnsii De Wild and Acacia karroo Hayne as reducing agent and as well as capping agent. The Acacia-mediated AgNPs were characterized with the use of UV-vis absorption spectroscopy, Fourier Transform Spectroscopy (FT-IR), Transmission electron microscope (TEM), Scanning Electron Microscope (SEM), Energy Dispersive Spectroscopy (EDX), and X-ray Diffractometry (XRD). A spherical, 10-40 nm diameter silver nanoparticles were synthesized with very low level of stability for the AMDS and the AKDS-AgNPs. In addition, nociceptive activity with a mice rat reveals higher inhibition at the neurogenic phase for the AKDS-AgNPs, while AMDS-AgNPs exhibited a high inhibition at the inflammatory phase. The potent anti-inflammatory activity of essential oils of A. mearnsii De Wild and A. karroo Hayne hereby confirmed its traditional use in treating various inflammatory diseases, while the inflammatory studies on the synthesized AgNPs reveals a very active compound which can be used as a potent opioid or non-steroidal anti-inflammatory drug (NSAID).
- Full Text:
- Date Issued: 2015
- Authors: Avoseh, Opeyemi Nudewhenu
- Date: 2015
- Language: English
- Type: Thesis , Doctoral , PhD (Chemistry)
- Identifier: vital:11355 , http://hdl.handle.net/10353/d1021292
- Description: Great wealth of traditional knowledge about the use of plants had been transferred from generation to generations leading to the present day drug discovery and invention of new scientific methods of isolation, purification and identification. With the discovery of new diseases and drug-resistant organisms, there is no other source or deposit of lead compounds or drugs than the plant kingdom. As a result of this, about 25% of the current drug administered owe their origin to plant sources with the view to reduce the carcinogenic effect of synthetic drugs. Volatile terpenoids among other broad spectrum of natural product had been implicated to show high therapeutic properly. In the present study, selected locally-used medicinal plants were exploited for the presence of potent bioactive compounds and ability to form nanoparticles with distinctive property for use as chemoprotective agent against inflammation, tumors, cancer and other chronic diseases. Acacia mearnsii De Wild and Acacia karroo Hayne studied in this report are known to be invasive species with no proper regulation to conserve and preserve them. However, ethnopharmacology report of these plant species in the Southern Africa region reveals that they are good antiseptic, anti-diarrhea, anti-inflammation and a forage for livestock. These plants were subjected to volatile extraction protocol of some parts of the plants (stem and leaves) followed by examination of the anti-inflammation capacity of the extracts using an animal model. In addition, the bye-product (hydrosol) from the stem bark of each species possess a high reducing and stabilizing property leading to synthesis of silver nanoparticles, followed by investigation of the anti-inflammation potential of the synthesized silver nanoparticles using animal model. The volatile oils of the leaves and stem bark of Acacia mearnsii De Wild obtained by hydro-distillation were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Twenty, Thirty-Eight, Twenty-nine and Thirty-Eight components accounting for 93.8%, 92.1%, 78.5% and 90.9% of the total oils of the fresh, dry leaves and fresh, dry stem bark respectively. The major components of the oil were octadecyl alcohol (25.5%) and phytol (10.5%); cis-verbenol (29.5%); phytol (10.1%) and phytol (23.4%) for the fresh leaves, dried leaves, and fresh stem, dry stem bark respectively. Oral administration of essential oils at the dose of 2% showed significant (p<0.05) anti-inflammatory properties in the albumin induced test model in rats. Oils from the fresh leaves and dry stems inhibited inflammation beyond 4 h post treatment. Furthermore, the chemical composition of the essential oils obtained by hydro-distillation from the leaves and stem bark (dry and fresh) of Acacia karroo Hayne, analysed by GC-MS, shows that hexanal (10.67%) and ß-ionone (9.74%) were dominant in the dried leaves, β-pinene (14.30%), and (Z)-2-Hexen-1-ol (10.21%) in the fresh leaves while Octacosane (10.59%) and phytol (23.38%) were dominant in the dry and fresh stem respectively. The anti-inflammation ability of these oils after an albumin-induced inflammation on wistar rats, shows a significant effect at the 1st h of treatment with a significance of P< 0.01 for all part plants, while the fresh leaves shows further inhibitory activities at the 2nd h of analysis. Silver nanoparticles (AgNPs) were successfully synthesized from AgNO3 through a green route using the aqueous extract (hydrosols) of Acacia mearnsii De Wild and Acacia karroo Hayne as reducing agent and as well as capping agent. The Acacia-mediated AgNPs were characterized with the use of UV-vis absorption spectroscopy, Fourier Transform Spectroscopy (FT-IR), Transmission electron microscope (TEM), Scanning Electron Microscope (SEM), Energy Dispersive Spectroscopy (EDX), and X-ray Diffractometry (XRD). A spherical, 10-40 nm diameter silver nanoparticles were synthesized with very low level of stability for the AMDS and the AKDS-AgNPs. In addition, nociceptive activity with a mice rat reveals higher inhibition at the neurogenic phase for the AKDS-AgNPs, while AMDS-AgNPs exhibited a high inhibition at the inflammatory phase. The potent anti-inflammatory activity of essential oils of A. mearnsii De Wild and A. karroo Hayne hereby confirmed its traditional use in treating various inflammatory diseases, while the inflammatory studies on the synthesized AgNPs reveals a very active compound which can be used as a potent opioid or non-steroidal anti-inflammatory drug (NSAID).
- Full Text:
- Date Issued: 2015
Determinants of internet banking adoption by banks in Ghana
- Authors: Bart-Williams, Edem
- Date: 2015
- Subjects: Internet banking -- Ghana , Banks and banking -- Ghana , Banks and banking -- Automation
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/3715 , vital:20457
- Description: Growth in information and communication technology (ICT) is drastically changing the way businesses, especially in the service industries, are conducted. The financial services industry and banking in particular, is not excluded from this technology explosion. Internet banking, even though not new in advanced countries, is a new transaction channel being used by banks in some parts of Africa, especially Ghana, to offer various products and services to their customers. However, this medium has not been fully exploited by these banks as there are many hurdles the banks must triumph over. In deploying this technology and these systems, there are several factors which banks must take into consideration before fully deploying such a system to their customers, hence the motivation for this study. The absence of suitable and sufficient knowledge on this topic also exposes a “rhetoric versus reality” argument of whether the intention to adopt Internet banking is critical to the strategies and ultimate success of banks in Ghana. For banks to stay ahead of competition as well as to attract and maintain their clientele, it is of paramount importance to gather and link the perspectives of both clients and bank managers in order for banks to ensure that they perform according to the needs and expectations of their clients. In order to achieve the intended results, an empirical study was conducted by taking into consideration the viewpoints of both bank clients and bank managers in determining the factors that customers take into consideration before adopting the Internet banking medium. The primary aim of this study was to quantify significant relationships between the selected variables. Therefore the positivism research paradigm was used, while the phenomenological paradigm was employed for the measuring instruments. Because multiple sources of data were used, from the perspectives of banking clients and managers in Ghana, methodological triangulation was adopted for this study. The results of the empirical investigation showed that both groups (clients and managers) considered the variables of market share, technology acceptance, diffusion of innovation, organisational variables, organisational efficiency, and business strategy to have direct influence on the adoption of Internet banking. However, they differed in opinion concerning the degree of influence of these variables. The bank managers’ responses leaned more towards strong agreement with the importance of these variables than did those of the bank clients. Thus, for bank clients to readily adopt the Internet banking medium for their banking transactions, bank managers must take a closer look at these determinant factors described in the study. The study showed that the population group, educational and income levels exerted an influence on the perceptions clients have regarding Internet banking adoption factors. It was found that the higher the education and income levels of the clients, the easier it was for them to adopt Internet banking. Also, the male group dominated the use of the Internet banking. This is supported by the fact that there is a growing middle class in Ghana that falls within this category of banking clients.
- Full Text:
- Date Issued: 2015
- Authors: Bart-Williams, Edem
- Date: 2015
- Subjects: Internet banking -- Ghana , Banks and banking -- Ghana , Banks and banking -- Automation
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/3715 , vital:20457
- Description: Growth in information and communication technology (ICT) is drastically changing the way businesses, especially in the service industries, are conducted. The financial services industry and banking in particular, is not excluded from this technology explosion. Internet banking, even though not new in advanced countries, is a new transaction channel being used by banks in some parts of Africa, especially Ghana, to offer various products and services to their customers. However, this medium has not been fully exploited by these banks as there are many hurdles the banks must triumph over. In deploying this technology and these systems, there are several factors which banks must take into consideration before fully deploying such a system to their customers, hence the motivation for this study. The absence of suitable and sufficient knowledge on this topic also exposes a “rhetoric versus reality” argument of whether the intention to adopt Internet banking is critical to the strategies and ultimate success of banks in Ghana. For banks to stay ahead of competition as well as to attract and maintain their clientele, it is of paramount importance to gather and link the perspectives of both clients and bank managers in order for banks to ensure that they perform according to the needs and expectations of their clients. In order to achieve the intended results, an empirical study was conducted by taking into consideration the viewpoints of both bank clients and bank managers in determining the factors that customers take into consideration before adopting the Internet banking medium. The primary aim of this study was to quantify significant relationships between the selected variables. Therefore the positivism research paradigm was used, while the phenomenological paradigm was employed for the measuring instruments. Because multiple sources of data were used, from the perspectives of banking clients and managers in Ghana, methodological triangulation was adopted for this study. The results of the empirical investigation showed that both groups (clients and managers) considered the variables of market share, technology acceptance, diffusion of innovation, organisational variables, organisational efficiency, and business strategy to have direct influence on the adoption of Internet banking. However, they differed in opinion concerning the degree of influence of these variables. The bank managers’ responses leaned more towards strong agreement with the importance of these variables than did those of the bank clients. Thus, for bank clients to readily adopt the Internet banking medium for their banking transactions, bank managers must take a closer look at these determinant factors described in the study. The study showed that the population group, educational and income levels exerted an influence on the perceptions clients have regarding Internet banking adoption factors. It was found that the higher the education and income levels of the clients, the easier it was for them to adopt Internet banking. Also, the male group dominated the use of the Internet banking. This is supported by the fact that there is a growing middle class in Ghana that falls within this category of banking clients.
- Full Text:
- Date Issued: 2015
Conditions enabling or constraining the exercise of agency among new academics in higher education, conducive to the social inclusion of students
- Authors: Behari-Leak, Kasturi
- Date: 2015
- Subjects: Social integration -- South Africa , Students -- South Africa -- Social conditions , Educational change -- South Africa , College teachers -- South Africa , Education, Higher -- South Africa , Critical realism , Social realism , Agent (Philosophy)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1333 , http://hdl.handle.net/10962/d1020295
- Description: This study, which is part of a National Research Foundation project on Social Inclusion in Higher Education (HE), focuses on the exercise of agency among new academics, conducive to the social inclusion of students. Transitioning from varied entry points into higher education, new academics face numerous challenges as they embed themselves in disciplinary and institutional contexts. Given the complexity and contested nature of the current higher education landscape, new academics are especially vulnerable. Using Roy Bhaskar’s critical realism as meta-theoretical framing and Margaret Archer’s social realist theory, with its methodological focus on analytical dualism and morphogenesis, this study offers a social realist account of how new academics engage with enabling and constraining conditions at institutional, faculty, departmental and classroom levels. Through an analysis of six individual narratives of mediation, this study explicates and exemplifies the range of agential choices exercised by new academics to mediate their contested spaces. A nuanced social and critical account of the material, ideational and agential conditions in HE shows that the courses of action taken by these new academics are driven through their concerns, commitments and projects in higher education. Yet, despite the university’s espousal of embracing change, the current induction and transition of new academics is inadequate to the task of transformation in higher education. Systemic conditions in HE, conducive to critical agency and social justice, are not enabling. Bhaskar’s Seven Scalar Being, used as an analytical frame and heuristic, guides the cross-case analysis of the six narratives across seven levels of ontology. The findings highlight that, despite difficult contextual influences, the positive exercise of agency is a marked feature of new participants in HE in this study. This has immediate implications for ways in which professional and academic development, and disciplinary and departmental programmes, could create and sustain conducive conditions for the professionalisation of new academics through more sensitised practices. Using alternative research methods such as photovoice to generate its data, this doctoral study proposes that new research methodologies, located in the third space, are needed now more than ever in HE sociological research, to recognise the researcher and the research participants as independent, autonomous and causally efficacious beings. To this end, this study includes a Chapter Zero, which captures the narrative of the doctoral scholar as researcher, who, shaped and influenced by established doctoral practices and traditions in the field, exercises her own doctoral agency in particular ways.
- Full Text:
- Date Issued: 2015
- Authors: Behari-Leak, Kasturi
- Date: 2015
- Subjects: Social integration -- South Africa , Students -- South Africa -- Social conditions , Educational change -- South Africa , College teachers -- South Africa , Education, Higher -- South Africa , Critical realism , Social realism , Agent (Philosophy)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1333 , http://hdl.handle.net/10962/d1020295
- Description: This study, which is part of a National Research Foundation project on Social Inclusion in Higher Education (HE), focuses on the exercise of agency among new academics, conducive to the social inclusion of students. Transitioning from varied entry points into higher education, new academics face numerous challenges as they embed themselves in disciplinary and institutional contexts. Given the complexity and contested nature of the current higher education landscape, new academics are especially vulnerable. Using Roy Bhaskar’s critical realism as meta-theoretical framing and Margaret Archer’s social realist theory, with its methodological focus on analytical dualism and morphogenesis, this study offers a social realist account of how new academics engage with enabling and constraining conditions at institutional, faculty, departmental and classroom levels. Through an analysis of six individual narratives of mediation, this study explicates and exemplifies the range of agential choices exercised by new academics to mediate their contested spaces. A nuanced social and critical account of the material, ideational and agential conditions in HE shows that the courses of action taken by these new academics are driven through their concerns, commitments and projects in higher education. Yet, despite the university’s espousal of embracing change, the current induction and transition of new academics is inadequate to the task of transformation in higher education. Systemic conditions in HE, conducive to critical agency and social justice, are not enabling. Bhaskar’s Seven Scalar Being, used as an analytical frame and heuristic, guides the cross-case analysis of the six narratives across seven levels of ontology. The findings highlight that, despite difficult contextual influences, the positive exercise of agency is a marked feature of new participants in HE in this study. This has immediate implications for ways in which professional and academic development, and disciplinary and departmental programmes, could create and sustain conducive conditions for the professionalisation of new academics through more sensitised practices. Using alternative research methods such as photovoice to generate its data, this doctoral study proposes that new research methodologies, located in the third space, are needed now more than ever in HE sociological research, to recognise the researcher and the research participants as independent, autonomous and causally efficacious beings. To this end, this study includes a Chapter Zero, which captures the narrative of the doctoral scholar as researcher, who, shaped and influenced by established doctoral practices and traditions in the field, exercises her own doctoral agency in particular ways.
- Full Text:
- Date Issued: 2015
The antifungal activity of an aqueous Tulbaghia violacea plant extract against Aspergillus flavus
- Authors: Belewa, Xoliswa Vuyokazi
- Date: 2015
- Subjects: Medicinal plants , Antifungal agents , Fungi -- Biotechnology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/5858 , vital:21001
- Description: Phytochemical analysis of both HEA1 and the crude plant extract showed the presence of phenolics, tannins and saponins. Saponins were the predominant secondary metabolites and were mostly abundant in the plant extract and to a lesser extent in the active compound. Steroidal saponins, tannins and phenolics were also detected in the plant extract, but only the phenolics were detected in the active compound. The results of the phytochemical analysis showed that those compounds that were not present in the active compound could be removed from the crude extract during the TLC purification process. Investigation on the mechanism of action of the crude plant extract on the sterol production by A. flavus showed that the plant extract affected ergosterol biosynthesis by causing an accumulation of oxidosqualene in the ergosterol biosynthetic pathway resulting in a decline in ergosterol production. An oscillatory response in lanosterol production was observed in the presence of the plant extract, which may be an adaptation mechanism of A. flavus to unfavourable conditions and compensation for the loss of enzyme activity which may have occurred as a result of the accumulation of oxidosqualene. The antifungal activity of the plant extract on ergosterol production by A. flavus may also be due to saponins which target the cell membrane and ergosterol production in fungi. The effect of the plant extract on the fungal cell wall of A. flavus also showed that the plant extract caused a decline in β-(1, 3) glucan production by inhibiting β-glucan synthase. The plant extract also affected the chitin synthesis pathway of A. flavus, by causing a decline in chitin production, which was due to the inhibition of chitin synthase. Investigation of chitinase production using 4MU substrates showed that the plant extract caused an accumulation of chitobioses, by activating chitobiosidases and endochitinases. A decline in N-acetylglucosaminidase activity in the presence of the plant extract was observed and this prevented the formation of N-acetylglucosamine. The accumulation of chitobiosidase and endochitinase may be as a result of autolysis that may be triggered by A. flavus as a survival mechanism in the presence of the plant extract and as a compensatory mechanism for the loss of β-glucans and chitin. The antifungal effect of the plant extract on various components of the cell wall of A. flavus, makes T. violacea aqueous plant extract an ideal chemotherapeutic agent against both human and plant pathogens of Aspergillus. The broad spectrum of antifungal activity of T. violacea against A. flavus also eliminates any chances of the fungus developing resistance towards it and would make it a candidate for use as a potential antifungal agent. Further identification and possible chemical synthesis is needed to shed light on the safety and efficacy of the active compound for further development as a chemotherapeutic agent.
- Full Text:
- Date Issued: 2015
- Authors: Belewa, Xoliswa Vuyokazi
- Date: 2015
- Subjects: Medicinal plants , Antifungal agents , Fungi -- Biotechnology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/5858 , vital:21001
- Description: Phytochemical analysis of both HEA1 and the crude plant extract showed the presence of phenolics, tannins and saponins. Saponins were the predominant secondary metabolites and were mostly abundant in the plant extract and to a lesser extent in the active compound. Steroidal saponins, tannins and phenolics were also detected in the plant extract, but only the phenolics were detected in the active compound. The results of the phytochemical analysis showed that those compounds that were not present in the active compound could be removed from the crude extract during the TLC purification process. Investigation on the mechanism of action of the crude plant extract on the sterol production by A. flavus showed that the plant extract affected ergosterol biosynthesis by causing an accumulation of oxidosqualene in the ergosterol biosynthetic pathway resulting in a decline in ergosterol production. An oscillatory response in lanosterol production was observed in the presence of the plant extract, which may be an adaptation mechanism of A. flavus to unfavourable conditions and compensation for the loss of enzyme activity which may have occurred as a result of the accumulation of oxidosqualene. The antifungal activity of the plant extract on ergosterol production by A. flavus may also be due to saponins which target the cell membrane and ergosterol production in fungi. The effect of the plant extract on the fungal cell wall of A. flavus also showed that the plant extract caused a decline in β-(1, 3) glucan production by inhibiting β-glucan synthase. The plant extract also affected the chitin synthesis pathway of A. flavus, by causing a decline in chitin production, which was due to the inhibition of chitin synthase. Investigation of chitinase production using 4MU substrates showed that the plant extract caused an accumulation of chitobioses, by activating chitobiosidases and endochitinases. A decline in N-acetylglucosaminidase activity in the presence of the plant extract was observed and this prevented the formation of N-acetylglucosamine. The accumulation of chitobiosidase and endochitinase may be as a result of autolysis that may be triggered by A. flavus as a survival mechanism in the presence of the plant extract and as a compensatory mechanism for the loss of β-glucans and chitin. The antifungal effect of the plant extract on various components of the cell wall of A. flavus, makes T. violacea aqueous plant extract an ideal chemotherapeutic agent against both human and plant pathogens of Aspergillus. The broad spectrum of antifungal activity of T. violacea against A. flavus also eliminates any chances of the fungus developing resistance towards it and would make it a candidate for use as a potential antifungal agent. Further identification and possible chemical synthesis is needed to shed light on the safety and efficacy of the active compound for further development as a chemotherapeutic agent.
- Full Text:
- Date Issued: 2015
Articulating the nature of clinical nurse specialist practice
- Authors: Bell, Janet Deanne
- Date: 2015
- Subjects: Nurse practitioners , Intensive care nursing
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:10057 , http://hdl.handle.net/10948/d1018623
- Description: Critical care nursing is a clinical specialist nursing practice discipline. The critical care nurse provides a constant presence in the care of a critically ill patient. She/he creates a thread of continuity in care through the myriad of other health care professionals and activities that form part of a patient’s stay in the critical care environment (World Federation of Critical Care Nurses [WFCCN], 2007). During conversations with people who have had intimate experience of the critical care environment, they have offered anecdotes that describe their interaction with critical care nurses who they perceive to be different from and better than other critical care nurses they encountered. Despite having met common professional requirements to be registered as a clinical specialist nurse, these distinctive, unique abilities that seem to be influential in meeting the complex needs and expectations of critically ill patients, their significant others as well as nursing and medical colleagues, are not displayed by all critical care nurses. While students of accredited postgraduate nursing programmes are required to advance their nursing knowledge and skill competence, many students do not seem to develop other, perhaps more tacit, qualities that utilisers characterise in their anecdotes of ‘different and better’ nursing practice. The overarching research question guiding this study was how can ‘different and better’ critical care nursing practice as recognised by a utiliser be explained? The purpose of this study was to develop an understanding of the qualities that those people who use critical care nursing practice recognise as ‘different and better’ to the norm of nursing practice they encounter in this discipline. The participant sample included patients’ significant others, nursing colleagues and medical colleagues of critical care nurses, collectively identified as utilisers. The stated aim of this work was to construct a grounded theory to elucidate an understanding of the qualities that a utiliser of critical care nursing recognises as ‘different and better’ critical care nursing practice in order to enhance the teaching and learning encounters between nurse educators and postgraduate students in learning programmes aiming to develop clinical specialist nurses. The method processes of grounded theory are designed to reveal and confirm concepts from within the data as well as the connections between these concepts, supporting the researcher in crafting a substantive theory that is definitively grounded in the participants’ views and stories (Streubert & Carpenter, 2011: 123, 128-129). Two data collection tools were employed in this study, namely in-depth unstructured individual interviews and naïve sketch. Constant comparative analysis, memo-writing, theoretical sampling, theoretical sensitivity and theoretical saturation as fundamental methods of data generation in grounded theory were applied. The study unfolded through three broad parts, namely: Forming & shaping this grounded theory through exploration and co-creation; Assimilating & situating this grounded theory through understanding and enfolding; Reflecting on this grounded theory through contemplating and reconnecting. The outcome of the first part of the study was my initial proposition of a grounded theory co-created in the interactions between the participants and myself. This was then challenged, developed and assimilated through a focussed literature review through the second part of the study. Through these two parts of this study, an inductively derived explanation was formed and shaped to produce an assimilated and situated substantive grounded theory named Being at Ease. This grounded theory articulates how ‘better and different’ nursing is recognised from the point of view of those who use the nursing ability of critical care nurses through the core concern ‘being at ease’ and its four categories ‘knowing self’, ‘skilled being’, connecting with intention’ and’ anchoring’. The final part of this study unfolded in my reflections on what this grounded theory had revealed about nurses and elements of nursing practice that are important to a utiliser in recognising ‘different and better’ critical care nursing. I suggest that as nurses we need to develop a language that enables us to reveal with clarity these intangible and tacit elements recognised within the being and doing of ‘different and better’ nursing. I reflected on the pivotal space of influence a teacher has with a student, and on how the elements essential in being and doing ‘different and better’ nursing need to be evident in her/his own ways of being a teacher of nursing. Teaching and learning encounters may be enhanced through drawing what this theory has shown as necessary elements that shape ‘different and better’ nurses through the moments of influence a teacher has in each encounter with a student.
- Full Text:
- Date Issued: 2015
- Authors: Bell, Janet Deanne
- Date: 2015
- Subjects: Nurse practitioners , Intensive care nursing
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:10057 , http://hdl.handle.net/10948/d1018623
- Description: Critical care nursing is a clinical specialist nursing practice discipline. The critical care nurse provides a constant presence in the care of a critically ill patient. She/he creates a thread of continuity in care through the myriad of other health care professionals and activities that form part of a patient’s stay in the critical care environment (World Federation of Critical Care Nurses [WFCCN], 2007). During conversations with people who have had intimate experience of the critical care environment, they have offered anecdotes that describe their interaction with critical care nurses who they perceive to be different from and better than other critical care nurses they encountered. Despite having met common professional requirements to be registered as a clinical specialist nurse, these distinctive, unique abilities that seem to be influential in meeting the complex needs and expectations of critically ill patients, their significant others as well as nursing and medical colleagues, are not displayed by all critical care nurses. While students of accredited postgraduate nursing programmes are required to advance their nursing knowledge and skill competence, many students do not seem to develop other, perhaps more tacit, qualities that utilisers characterise in their anecdotes of ‘different and better’ nursing practice. The overarching research question guiding this study was how can ‘different and better’ critical care nursing practice as recognised by a utiliser be explained? The purpose of this study was to develop an understanding of the qualities that those people who use critical care nursing practice recognise as ‘different and better’ to the norm of nursing practice they encounter in this discipline. The participant sample included patients’ significant others, nursing colleagues and medical colleagues of critical care nurses, collectively identified as utilisers. The stated aim of this work was to construct a grounded theory to elucidate an understanding of the qualities that a utiliser of critical care nursing recognises as ‘different and better’ critical care nursing practice in order to enhance the teaching and learning encounters between nurse educators and postgraduate students in learning programmes aiming to develop clinical specialist nurses. The method processes of grounded theory are designed to reveal and confirm concepts from within the data as well as the connections between these concepts, supporting the researcher in crafting a substantive theory that is definitively grounded in the participants’ views and stories (Streubert & Carpenter, 2011: 123, 128-129). Two data collection tools were employed in this study, namely in-depth unstructured individual interviews and naïve sketch. Constant comparative analysis, memo-writing, theoretical sampling, theoretical sensitivity and theoretical saturation as fundamental methods of data generation in grounded theory were applied. The study unfolded through three broad parts, namely: Forming & shaping this grounded theory through exploration and co-creation; Assimilating & situating this grounded theory through understanding and enfolding; Reflecting on this grounded theory through contemplating and reconnecting. The outcome of the first part of the study was my initial proposition of a grounded theory co-created in the interactions between the participants and myself. This was then challenged, developed and assimilated through a focussed literature review through the second part of the study. Through these two parts of this study, an inductively derived explanation was formed and shaped to produce an assimilated and situated substantive grounded theory named Being at Ease. This grounded theory articulates how ‘better and different’ nursing is recognised from the point of view of those who use the nursing ability of critical care nurses through the core concern ‘being at ease’ and its four categories ‘knowing self’, ‘skilled being’, connecting with intention’ and’ anchoring’. The final part of this study unfolded in my reflections on what this grounded theory had revealed about nurses and elements of nursing practice that are important to a utiliser in recognising ‘different and better’ critical care nursing. I suggest that as nurses we need to develop a language that enables us to reveal with clarity these intangible and tacit elements recognised within the being and doing of ‘different and better’ nursing. I reflected on the pivotal space of influence a teacher has with a student, and on how the elements essential in being and doing ‘different and better’ nursing need to be evident in her/his own ways of being a teacher of nursing. Teaching and learning encounters may be enhanced through drawing what this theory has shown as necessary elements that shape ‘different and better’ nurses through the moments of influence a teacher has in each encounter with a student.
- Full Text:
- Date Issued: 2015
Drivers and consequences of residents' satisfaction with off-campus student housing in South-South, Nigeria
- Authors: Bella-Omunagbe, Ojo Cyprain
- Date: 2015
- Subjects: Student housing -- Nigeria , Housing -- Resident satisfaction , Residence and education , Universities and colleges -- Nigeria -- Administration
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9720 , http://hdl.handle.net/10948/d1021198
- Description: The student housing system worldwide and South-South Nigeria in particular has witnessed an unprecedented transformation, such that private off-campus student housing facilities (SHFs) are now the primary source of accommodation for students in tertiary institutions. A considerable gap exists between the supply and demand for on-campus student housing and the quest to fill this gap has stimulated the creation of a significant student housing market in the areas where these tertiary institutions are located. The prospect for economic investments in the student housing sector is high and private investors are involved in the provision and management of offcampus student housing. The main consequence of this practice in South-South, Nigeria is the delivery of low-quality buildings that are not able to meet the needs and expectations of residents. SHFs that are constructed without due regard to residents needs are characterised by dissatisfaction with attributes of housing and low investment performance. The implication is that residence users are often not satisfied with the attributes of the residential environment that are provided; thus their behaviours often impose some consequences on investors gains and objectives.Therefore, understanding the dynamics among attributes that are important to students, that give the required satisfaction, and the impact of the availability or lack-of on behaviour such as loyalty, willingness to pay for attributes and word of mouth behaviour are critical to profitability. Most often, the relationship among these attributes are treated as linear and symmetrical with the assumed implication that better attributes produce improved behaviours. However, this may not always be the case. This approach is rarely addressed and is little understood in student housing studies. Therefore, the aim of this study was to identify student housing attributes that act as drivers of resident atisfaction and the consequences/effects of these drivers on student behaviour in order to determine appropriate measures that could be used to develop, maintain and upgrade student accommodation. The methodology of the study included an extensive literature review and a field study conducted to obtain the perceptions of students in seven tertiary institutions located in South-South, Nigeria. The main task was to define attributes of student housing facilities based on the symmetric and asymmetric impact of the performance of attributes on satisfaction with residence. The Kano model and importance-performance analysis (IPA) were used to establish sets of criteria that could be used to prioritise attributes that are required in student housing for optimal investor gains. Analysis of the findings lead to the conclusions that different degrees of behaviour were associated to the perception of importance that is attached to attributes by residents and the satisfaction that is derived from the use of such attributes. The implication of the conclusions is that to meet users satisfaction needs, varied improvement strategies are required for different attributes in order to maximise the use of resources for maximum gains. The recommendations for investors in SHFs include among others to segment the SHFs market based on demographic characteristics, prioritise and provide only attributes that add-value to identified groups. Emphasis should also be placed on providing attributes that are not only satisfactory, but with capacity to improve loyalty/retention, willingness to pay and positive word of mouth behaviour. It is also recommended that the local authority should improve critical attributes that are deemed to be outside the scope of the investors.
- Full Text:
- Date Issued: 2015
- Authors: Bella-Omunagbe, Ojo Cyprain
- Date: 2015
- Subjects: Student housing -- Nigeria , Housing -- Resident satisfaction , Residence and education , Universities and colleges -- Nigeria -- Administration
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9720 , http://hdl.handle.net/10948/d1021198
- Description: The student housing system worldwide and South-South Nigeria in particular has witnessed an unprecedented transformation, such that private off-campus student housing facilities (SHFs) are now the primary source of accommodation for students in tertiary institutions. A considerable gap exists between the supply and demand for on-campus student housing and the quest to fill this gap has stimulated the creation of a significant student housing market in the areas where these tertiary institutions are located. The prospect for economic investments in the student housing sector is high and private investors are involved in the provision and management of offcampus student housing. The main consequence of this practice in South-South, Nigeria is the delivery of low-quality buildings that are not able to meet the needs and expectations of residents. SHFs that are constructed without due regard to residents needs are characterised by dissatisfaction with attributes of housing and low investment performance. The implication is that residence users are often not satisfied with the attributes of the residential environment that are provided; thus their behaviours often impose some consequences on investors gains and objectives.Therefore, understanding the dynamics among attributes that are important to students, that give the required satisfaction, and the impact of the availability or lack-of on behaviour such as loyalty, willingness to pay for attributes and word of mouth behaviour are critical to profitability. Most often, the relationship among these attributes are treated as linear and symmetrical with the assumed implication that better attributes produce improved behaviours. However, this may not always be the case. This approach is rarely addressed and is little understood in student housing studies. Therefore, the aim of this study was to identify student housing attributes that act as drivers of resident atisfaction and the consequences/effects of these drivers on student behaviour in order to determine appropriate measures that could be used to develop, maintain and upgrade student accommodation. The methodology of the study included an extensive literature review and a field study conducted to obtain the perceptions of students in seven tertiary institutions located in South-South, Nigeria. The main task was to define attributes of student housing facilities based on the symmetric and asymmetric impact of the performance of attributes on satisfaction with residence. The Kano model and importance-performance analysis (IPA) were used to establish sets of criteria that could be used to prioritise attributes that are required in student housing for optimal investor gains. Analysis of the findings lead to the conclusions that different degrees of behaviour were associated to the perception of importance that is attached to attributes by residents and the satisfaction that is derived from the use of such attributes. The implication of the conclusions is that to meet users satisfaction needs, varied improvement strategies are required for different attributes in order to maximise the use of resources for maximum gains. The recommendations for investors in SHFs include among others to segment the SHFs market based on demographic characteristics, prioritise and provide only attributes that add-value to identified groups. Emphasis should also be placed on providing attributes that are not only satisfactory, but with capacity to improve loyalty/retention, willingness to pay and positive word of mouth behaviour. It is also recommended that the local authority should improve critical attributes that are deemed to be outside the scope of the investors.
- Full Text:
- Date Issued: 2015
Spatial and temporal variations in trophic connectivity within an estuarine environment : benthic-pelagic and terrestrial-aquatic linkages via invertebrates and fishes
- Authors: Bergamino Roman, Leandro
- Date: 2015
- Subjects: Estuarine ecology -- South Africa , Marine invertebrates -- South Africa -- Ecology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5921 , http://hdl.handle.net/10962/d1017799
- Description: Estuarine ecosystems are among the most biologically productive areas and they provide important ecosystem services such as erosion control, habitat and refugia for several species. These environments are characterized by the presence of a variety of organic matter sources due to their transitional position between rivers and the sea. The biotic compositions can undergo spatial and seasonal changes along the estuary due to the spatial and temporal fluctuations of environmental factors such as salinity, temperature and seston loads. Therefore, the different combinations of biotic and abiotic factors make each estuary a unique ecosystem. Because of this spatial and temporal complexity, the understanding of estuarine food web structure and which factors affect the trophic relationships within the ecosystem through space and time represent challenging tasks. Furthermore, estuaries are under an increasing number of anthropogenic perturbations because of the growing concentration of human populations in coastal areas. Knowledge of ecosystem structure and functioning is essential for effective conservation and management planning of coastal areas.In this dissertation, I combine the utilization of biological tracers to examine spatial and temporal variability in the food web structure within a small temperate and microtidal estuary located in South Africa. To this end, fatty acid profiles and stable isotope signatures were measured in several primary organic matter sources and consumers (including zooplankton, fishes and benthic invertebrates) during four consecutive seasons and in three different estuarine regions: upper, middle, and lower reaches. The three reaches had distinct habitat features of vegetation type and morphology, and in particular the lower reaches were colonized by the marsh grass Spartina maritima. Isotopic mixing models were used to estimate the relative contribution of each food source to the diets of invertebrates and fishes within the estuarine food web. The isotopic and fatty acid data showed similar results. In general, the lower reaches of the estuary were characterized by a higher deposition and assimilation by brachyuran crabs of carbon derived from marsh grass detritus, whiletowards the upper reaches a mixture of microphytobenthos and particulate organic matter (phytoplankton and detritus) was deposited and sustained the pelagic and benthic fauna. The highest deposition and assimilation of marsh grass detritus in the lower reaches of the estuary occurred during periods of low freshwater discharge (autumn and winter). In the upper reaches, microphytobenthos and suspended particulate organic matter were dominant basal food resources for the food web during all seasons. These results indicated that benthic consumers incorporated mainly local carbon sources from their local habitat.To clarify isotopic and fatty acid patterns I examined the trophic behaviour of the sesarmid crab Sesarma catenata through laboratory feeding experiments. Results from these experiments validated that decomposed leaves of riparian trees and the salt marsh plant S. maritima were the preferred food of the sesarmid crabs, potentially due to high bacterial loads. The remaining leaf material not assimilated by crabs, together with faecal material, are likely important subsidies for adjacent environments, hence representing an important energy pathway involving the microbial food chain. Furthermore, this dissertation showed the importance of mobile top predators as vectors energetically connecting distinct food chains within the estuary (i.e. littoral, benthic and pelagic). I concluded that a combination of physical (i.e. patterns of freshwater discharge and estuary morphology) and biological factors (i.e. organism feeding behaviour, mobility, primary productivity, the local vegetation type) influence the pattern of dominant primary organic matter sources, and therefore the food web structure along the estuarine environment. In particular, marsh grass detritus contributed substantially to the diets of estuarine fauna during periods of low freshwater discharge. Given the importance of the salt marsh habitat in providing trophic resources, it is important to preserve this environment to sustain the natural biota and ecosystem functioning.
- Full Text:
- Date Issued: 2015
- Authors: Bergamino Roman, Leandro
- Date: 2015
- Subjects: Estuarine ecology -- South Africa , Marine invertebrates -- South Africa -- Ecology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5921 , http://hdl.handle.net/10962/d1017799
- Description: Estuarine ecosystems are among the most biologically productive areas and they provide important ecosystem services such as erosion control, habitat and refugia for several species. These environments are characterized by the presence of a variety of organic matter sources due to their transitional position between rivers and the sea. The biotic compositions can undergo spatial and seasonal changes along the estuary due to the spatial and temporal fluctuations of environmental factors such as salinity, temperature and seston loads. Therefore, the different combinations of biotic and abiotic factors make each estuary a unique ecosystem. Because of this spatial and temporal complexity, the understanding of estuarine food web structure and which factors affect the trophic relationships within the ecosystem through space and time represent challenging tasks. Furthermore, estuaries are under an increasing number of anthropogenic perturbations because of the growing concentration of human populations in coastal areas. Knowledge of ecosystem structure and functioning is essential for effective conservation and management planning of coastal areas.In this dissertation, I combine the utilization of biological tracers to examine spatial and temporal variability in the food web structure within a small temperate and microtidal estuary located in South Africa. To this end, fatty acid profiles and stable isotope signatures were measured in several primary organic matter sources and consumers (including zooplankton, fishes and benthic invertebrates) during four consecutive seasons and in three different estuarine regions: upper, middle, and lower reaches. The three reaches had distinct habitat features of vegetation type and morphology, and in particular the lower reaches were colonized by the marsh grass Spartina maritima. Isotopic mixing models were used to estimate the relative contribution of each food source to the diets of invertebrates and fishes within the estuarine food web. The isotopic and fatty acid data showed similar results. In general, the lower reaches of the estuary were characterized by a higher deposition and assimilation by brachyuran crabs of carbon derived from marsh grass detritus, whiletowards the upper reaches a mixture of microphytobenthos and particulate organic matter (phytoplankton and detritus) was deposited and sustained the pelagic and benthic fauna. The highest deposition and assimilation of marsh grass detritus in the lower reaches of the estuary occurred during periods of low freshwater discharge (autumn and winter). In the upper reaches, microphytobenthos and suspended particulate organic matter were dominant basal food resources for the food web during all seasons. These results indicated that benthic consumers incorporated mainly local carbon sources from their local habitat.To clarify isotopic and fatty acid patterns I examined the trophic behaviour of the sesarmid crab Sesarma catenata through laboratory feeding experiments. Results from these experiments validated that decomposed leaves of riparian trees and the salt marsh plant S. maritima were the preferred food of the sesarmid crabs, potentially due to high bacterial loads. The remaining leaf material not assimilated by crabs, together with faecal material, are likely important subsidies for adjacent environments, hence representing an important energy pathway involving the microbial food chain. Furthermore, this dissertation showed the importance of mobile top predators as vectors energetically connecting distinct food chains within the estuary (i.e. littoral, benthic and pelagic). I concluded that a combination of physical (i.e. patterns of freshwater discharge and estuary morphology) and biological factors (i.e. organism feeding behaviour, mobility, primary productivity, the local vegetation type) influence the pattern of dominant primary organic matter sources, and therefore the food web structure along the estuarine environment. In particular, marsh grass detritus contributed substantially to the diets of estuarine fauna during periods of low freshwater discharge. Given the importance of the salt marsh habitat in providing trophic resources, it is important to preserve this environment to sustain the natural biota and ecosystem functioning.
- Full Text:
- Date Issued: 2015
Understanding climate variability and livelihoods adaptation in rural Zimbabwe : case of Charewa, Mutoko
- Authors: Bhatasara, Sandra
- Date: 2015
- Subjects: Climatic changes -- Zimbabwe -- Mutoko , Sustainable agriculture -- Zimbabwe -- Mutoko , Environmental impact analysis -- Zimbabwe -- Mutoko , Farmers -- Zimbabwe -- Mutoko -- Economic conditions , Food supply -- Zimbabwe -- Mutoko , Farms -- Zimbabwe -- Mutoko , Land use, Rural -- Zimbabwe -- Mutoko , Crops and climate -- Zimbabwe -- Mutoko
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3403 , http://hdl.handle.net/10962/d1018928 , Climatic changes -- Zimbabwe -- Mutoko , Sustainable agriculture -- Zimbabwe -- Mutoko , Environmental impact analysis -- Zimbabwe -- Mutoko , Farmers -- Zimbabwe -- Mutoko -- Economic conditions , Food supply -- Zimbabwe -- Mutoko , Farms -- Zimbabwe -- Mutoko , Land use, Rural -- Zimbabwe -- Mutoko , Crops and climate -- Zimbabwe -- Mutoko
- Description: Rural farmers in Zimbabwe have been grappling with various changes and challenges occurring in the country since the early 1990s. Amongst these, climate variability has emerged as one significant aspect. It has introduced new challenges for these farmers who are already facing various difficulties in maintaining their insecure livelihoods. Yet, current adaptation theories and inquiries have failed to sufficiently account for and analyse the capacity of these farmers to adequately respond to changing climatic conditions. In this respect, a number of studies have been heavily embedded in deterministic concepts that regard rural farmers as passive victims who play only a minor part in decisions and actions that affect their own livelihoods and well-being. Similarly, although some studies have acknowledged farmers’ capacity to adapt and build elements of resilience, they have not adequately shown how farmers interpret changes in climate and the structures, processes and conditions underpinning adaptation. Following that, my study uses a case study of a rural community in a semi-arid region of Mutoko district in eastern Zimbabwe and Margaret Archer’s sociological theory to understand and analyse how farmers problematise climate variability and respond to it. The study utilises a qualitative approach to divulge the subtleties on how rural people interpret processes of change and adapt to such changes. The thesis found that farmers are encountering increasingly unpredictable and unreliable rainfall patterns as well as shifting temperature conditions which are inducing labyrinthian livelihoods conundrums. However, these climatic shifts are not being experienced in a discrete manner hence farmers are also discontented with the obtaining socio-economic circumstances in the country. Simultaneously, whilst farmers in large part conceived changes in rainfall and temperature to be caused by natural shifts in climate, they also ascribed them to cultural and religious facets. Importantly, the thesis reveals considerable resourcefulness by farmers in the face of nascent changes in climate variability. Farmers have therefore constructed versatile coping and adaptive strategies. What is crucial to mention here is that climatic and non-climatic challenges are negotiated concurrently. Therein, farmers are adapting to climate variability and at the same time navigating difficult socio-economic landscapes. All the same, the process of adaptation is ostensibly not straightforward but complex. As it evolves, farmers find themselves facing numerous constraining structures and processes. Nonetheless, farmers in this study are able to circumvent the constraints presented to them and at the same time activate the corresponding enabling structures, processes and conditions.
- Full Text:
- Date Issued: 2015
- Authors: Bhatasara, Sandra
- Date: 2015
- Subjects: Climatic changes -- Zimbabwe -- Mutoko , Sustainable agriculture -- Zimbabwe -- Mutoko , Environmental impact analysis -- Zimbabwe -- Mutoko , Farmers -- Zimbabwe -- Mutoko -- Economic conditions , Food supply -- Zimbabwe -- Mutoko , Farms -- Zimbabwe -- Mutoko , Land use, Rural -- Zimbabwe -- Mutoko , Crops and climate -- Zimbabwe -- Mutoko
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3403 , http://hdl.handle.net/10962/d1018928 , Climatic changes -- Zimbabwe -- Mutoko , Sustainable agriculture -- Zimbabwe -- Mutoko , Environmental impact analysis -- Zimbabwe -- Mutoko , Farmers -- Zimbabwe -- Mutoko -- Economic conditions , Food supply -- Zimbabwe -- Mutoko , Farms -- Zimbabwe -- Mutoko , Land use, Rural -- Zimbabwe -- Mutoko , Crops and climate -- Zimbabwe -- Mutoko
- Description: Rural farmers in Zimbabwe have been grappling with various changes and challenges occurring in the country since the early 1990s. Amongst these, climate variability has emerged as one significant aspect. It has introduced new challenges for these farmers who are already facing various difficulties in maintaining their insecure livelihoods. Yet, current adaptation theories and inquiries have failed to sufficiently account for and analyse the capacity of these farmers to adequately respond to changing climatic conditions. In this respect, a number of studies have been heavily embedded in deterministic concepts that regard rural farmers as passive victims who play only a minor part in decisions and actions that affect their own livelihoods and well-being. Similarly, although some studies have acknowledged farmers’ capacity to adapt and build elements of resilience, they have not adequately shown how farmers interpret changes in climate and the structures, processes and conditions underpinning adaptation. Following that, my study uses a case study of a rural community in a semi-arid region of Mutoko district in eastern Zimbabwe and Margaret Archer’s sociological theory to understand and analyse how farmers problematise climate variability and respond to it. The study utilises a qualitative approach to divulge the subtleties on how rural people interpret processes of change and adapt to such changes. The thesis found that farmers are encountering increasingly unpredictable and unreliable rainfall patterns as well as shifting temperature conditions which are inducing labyrinthian livelihoods conundrums. However, these climatic shifts are not being experienced in a discrete manner hence farmers are also discontented with the obtaining socio-economic circumstances in the country. Simultaneously, whilst farmers in large part conceived changes in rainfall and temperature to be caused by natural shifts in climate, they also ascribed them to cultural and religious facets. Importantly, the thesis reveals considerable resourcefulness by farmers in the face of nascent changes in climate variability. Farmers have therefore constructed versatile coping and adaptive strategies. What is crucial to mention here is that climatic and non-climatic challenges are negotiated concurrently. Therein, farmers are adapting to climate variability and at the same time navigating difficult socio-economic landscapes. All the same, the process of adaptation is ostensibly not straightforward but complex. As it evolves, farmers find themselves facing numerous constraining structures and processes. Nonetheless, farmers in this study are able to circumvent the constraints presented to them and at the same time activate the corresponding enabling structures, processes and conditions.
- Full Text:
- Date Issued: 2015
Invariant control systems and sub-Riemannian structures on lie groups: equivalence and isometries
- Authors: Biggs, Rory
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64815 , vital:28607
- Description: In this thesis we consider invariant optimal control problems and invariant sub-Riemannian structures on Lie groups. Primarily, we are concerned with the equivalence and classification of problems (resp. structures). In the first chapter, both the class of invariant optimal control problems and the class of invariant sub-Riemannian structures are organised as categories. The latter category is shown to be functorially equivalent to a subcategory of the former category. Via the Pontryagin Maximum Principle, we associate to each invariant optimal control problem (resp. invariant sub-Riemannian structure) a quadratic Hamilton-Poisson system on the associated Lie-Poisson space. These Hamiltonian systems are also organised as a category and hence the Pontryagin lift is realised as a contravariant functor. Basic properties of these categories are investigated. The rest of this thesis is concerned with the classification (and investigation) of certain subclasses of structures and systems. In the second chapter, the homogeneous positive semidefinite quadratic Hamilton-Poisson systems on three-dimensional Lie-Poisson spaces are classified up to compatibility with a linear isomorphism; a list of 23 normal forms is exhibited. In the third chapter, we classify the invariant sub-Riemannian structures in three dimensions and calculate the isometry group for each normal form. By comparing our results with known results, we show that most isometries (in three dimensions) are in fact the composition of a left translation and a Lie group isomorphism. In the fourth and last chapter of this thesis, we classify the sub-Riemannian and Riemannian structures on the (2n + 1)-dimensional Heisenberg groups. Furthermore, we find the isometry group and geodesics of each normal form.
- Full Text:
- Date Issued: 2015
- Authors: Biggs, Rory
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64815 , vital:28607
- Description: In this thesis we consider invariant optimal control problems and invariant sub-Riemannian structures on Lie groups. Primarily, we are concerned with the equivalence and classification of problems (resp. structures). In the first chapter, both the class of invariant optimal control problems and the class of invariant sub-Riemannian structures are organised as categories. The latter category is shown to be functorially equivalent to a subcategory of the former category. Via the Pontryagin Maximum Principle, we associate to each invariant optimal control problem (resp. invariant sub-Riemannian structure) a quadratic Hamilton-Poisson system on the associated Lie-Poisson space. These Hamiltonian systems are also organised as a category and hence the Pontryagin lift is realised as a contravariant functor. Basic properties of these categories are investigated. The rest of this thesis is concerned with the classification (and investigation) of certain subclasses of structures and systems. In the second chapter, the homogeneous positive semidefinite quadratic Hamilton-Poisson systems on three-dimensional Lie-Poisson spaces are classified up to compatibility with a linear isomorphism; a list of 23 normal forms is exhibited. In the third chapter, we classify the invariant sub-Riemannian structures in three dimensions and calculate the isometry group for each normal form. By comparing our results with known results, we show that most isometries (in three dimensions) are in fact the composition of a left translation and a Lie group isomorphism. In the fourth and last chapter of this thesis, we classify the sub-Riemannian and Riemannian structures on the (2n + 1)-dimensional Heisenberg groups. Furthermore, we find the isometry group and geodesics of each normal form.
- Full Text:
- Date Issued: 2015
The diversity of root fungi associated with Erica species occurring in the Albany Centre of Endemism
- Authors: Bizabani, Christine
- Date: 2015
- Subjects: Ericaceae , Ericas , Roots (Botany) -- Diseases and pests , Mycorrhizal fungi , Polymerase chain reaction , Fungi -- Classification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4160 , http://hdl.handle.net/10962/d1018575
- Description: South Africa has the highest species diversity of ericaceous plants belonging to the Erica genus. There are over 850 identified species in the Cape Floral Region. The Albany Centre of Endemism (ACOE) is located within this region and is a hotspot of diversity consisting of various plant genera. The success of Erica plants is ubiquitously attributed to mycorrhizal relationships they engage in with a diverse group of fungi. This symbiosis is known as the ericoid mycorrhizal (ERM) association. The overall aim of this study was to establish the diversity of root fungi associated with Erica plants using morphological, molecular and 454 pyrosequencing techniques. Six Erica species were identified using leaf and flower morphology according to taxonomic keys. The identified plants were Erica cerinthoides, Erica demissa, Erica chamissonis, Erica glumiflora, Erica caffra and Erica nemorosa. Roots from sampled plants were stained and examined microscopically to determine their mycorrhizal status. Ericoid mycorrhizal associations together with dark septate endophyte (DSE) structures and hyphae that did not form any specific structure were observed in all the roots. In addition arbuscular mycorrhizal (AM) structures in the form of vesicles were detected in E. glumiflora and E. cerinthoides. In order to identify the culturable fungi associated with the respective hosts, sterilised roots were placed on various culture media for cultivation. Thereafter isolated fungi were morphologically classified into 67 morphotypes. These were mostly sterile and darkly pigmented. Non-sporulating mycelia of variable colouration such as white, cream-yellowish, beige, green and brown were also observed. Further identification was carried out using molecular techniques. DNA was extracted separately from pure cultures and amplified using ITS1 and ITS4 primers in a polymerase chain reaction (PCR). Thereafter sequencing and Basic Local Alignment Search Tool (BLAST) were used to identify the isolates to generic level. The fungi were taxonomically classified into 54 operational taxonomic units and 94 percent were Ascomycetes and Helotiales was the dominant order. Unclassified Helotiales with affinities to fungi currently identified as Epacrid root fungus was common in all hosts. Other isolates that were identified included Oidiodendron, Meliniomyces, Phialocephala, Cadophora, Lachnum, Leohumicola Cryptosporiopsis, Chaetomium, Acremonium and Epicoccum species. Basidiomycetes were represented by two OTUs belonging to the genus Mycena. Four OTUs comprised fungi that had no significant alignments in the reference databases. Direct root DNA extraction together with 454 pyrosequencing was used to detect the diversity of culturable and unculturable fungi associated with the identified hosts. The ITS2 region was targeted for sequencing. Although Ascomycetes remained the dominant phyla, Basidiomycetes were also detected in all host plants. Glomeromycota was present in E. caffra and E. cerinthoides. Helotiales was dominant in all Erica plants with the exception of E. cerinthoides and E. chamissonis which were dominated by the order Chaetothyriales. The OTUs identified to genus level included Epacris pulchella root fungus, Oidiodendron cf. maius, Acremonium implicatum, Leohumicola, Lachnum, Capronia and Mycena species. Culture-based techniques and pyrosequencing detected similar fungal composition comprising Ascomycetes, while, pyrosequencing was able to detect Glomeromycetes and Basidiomycetes.
- Full Text:
- Date Issued: 2015
The diversity of root fungi associated with Erica species occurring in the Albany Centre of Endemism
- Authors: Bizabani, Christine
- Date: 2015
- Subjects: Ericaceae , Ericas , Roots (Botany) -- Diseases and pests , Mycorrhizal fungi , Polymerase chain reaction , Fungi -- Classification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4160 , http://hdl.handle.net/10962/d1018575
- Description: South Africa has the highest species diversity of ericaceous plants belonging to the Erica genus. There are over 850 identified species in the Cape Floral Region. The Albany Centre of Endemism (ACOE) is located within this region and is a hotspot of diversity consisting of various plant genera. The success of Erica plants is ubiquitously attributed to mycorrhizal relationships they engage in with a diverse group of fungi. This symbiosis is known as the ericoid mycorrhizal (ERM) association. The overall aim of this study was to establish the diversity of root fungi associated with Erica plants using morphological, molecular and 454 pyrosequencing techniques. Six Erica species were identified using leaf and flower morphology according to taxonomic keys. The identified plants were Erica cerinthoides, Erica demissa, Erica chamissonis, Erica glumiflora, Erica caffra and Erica nemorosa. Roots from sampled plants were stained and examined microscopically to determine their mycorrhizal status. Ericoid mycorrhizal associations together with dark septate endophyte (DSE) structures and hyphae that did not form any specific structure were observed in all the roots. In addition arbuscular mycorrhizal (AM) structures in the form of vesicles were detected in E. glumiflora and E. cerinthoides. In order to identify the culturable fungi associated with the respective hosts, sterilised roots were placed on various culture media for cultivation. Thereafter isolated fungi were morphologically classified into 67 morphotypes. These were mostly sterile and darkly pigmented. Non-sporulating mycelia of variable colouration such as white, cream-yellowish, beige, green and brown were also observed. Further identification was carried out using molecular techniques. DNA was extracted separately from pure cultures and amplified using ITS1 and ITS4 primers in a polymerase chain reaction (PCR). Thereafter sequencing and Basic Local Alignment Search Tool (BLAST) were used to identify the isolates to generic level. The fungi were taxonomically classified into 54 operational taxonomic units and 94 percent were Ascomycetes and Helotiales was the dominant order. Unclassified Helotiales with affinities to fungi currently identified as Epacrid root fungus was common in all hosts. Other isolates that were identified included Oidiodendron, Meliniomyces, Phialocephala, Cadophora, Lachnum, Leohumicola Cryptosporiopsis, Chaetomium, Acremonium and Epicoccum species. Basidiomycetes were represented by two OTUs belonging to the genus Mycena. Four OTUs comprised fungi that had no significant alignments in the reference databases. Direct root DNA extraction together with 454 pyrosequencing was used to detect the diversity of culturable and unculturable fungi associated with the identified hosts. The ITS2 region was targeted for sequencing. Although Ascomycetes remained the dominant phyla, Basidiomycetes were also detected in all host plants. Glomeromycota was present in E. caffra and E. cerinthoides. Helotiales was dominant in all Erica plants with the exception of E. cerinthoides and E. chamissonis which were dominated by the order Chaetothyriales. The OTUs identified to genus level included Epacris pulchella root fungus, Oidiodendron cf. maius, Acremonium implicatum, Leohumicola, Lachnum, Capronia and Mycena species. Culture-based techniques and pyrosequencing detected similar fungal composition comprising Ascomycetes, while, pyrosequencing was able to detect Glomeromycetes and Basidiomycetes.
- Full Text:
- Date Issued: 2015
A multiscale remote sensing assessment of subtropical indigenous forests along the wild coast, South Africa
- Authors: Blessing, Sithole Vhusomuzi
- Date: 2015
- Subjects: Forests and forestry -- South Africa -- Remote sensing , Forest conservation , Remote sensing , Geographic information systems
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10677 , http://hdl.handle.net/10948/d1021169
- Description: The subtropical forests located along South Africa’s Wild Coast region, declared as one of the biodiversity hotspots, provide benefits to the local and national economy. However, there is evidence of increased pressure exerted on the forests by growing population and reduced income from activities not related to forest products. The ability of remote sensing to quantify subtropical forest changes over time, perform species discrimination (using field spectroscopy) and integrating field spectral and multispectral data were all assessed in this study. Investigations were conducted at pixel, leaf and sub-pixel levels. Both per-pixel and sub-pixel classification methods were used for improved forest characterisation. Using SPOT 6 imagery for 2013, the study determined the best classification algorithm for mapping sub-tropical forest and other land cover types to be the maximum likelihood classifier. Maximum likelihood outperformed minimum distance, spectral angle mapper and spectral information divergence algorithms, based on overall accuracy and Kappa coefficient values. Forest change analysis was made based on spectral measurements made at top of the atmosphere (TOC) level. When applied to the 2005 and 2009 SPOT 5 images, subtropical forest changes between 2005-2009 and 2009-2013 were quantified. A temporal analysis of forest cover trends in the periods 2005-2009 and 2009-2013 identified a decreasing trend of -3648.42 and -946.98 ha respectively, which translated to 7.81 percent and 2.20 percent decrease. Although there is evidence of a trend towards decreased rates of forest loss, more conservation efforts are required to protect the Wild Coast ecosystem. Using field spectral measurements data, the hierarchical method (comprising One-way ANOVA with Bonferroni correction, Classification and Regression Trees (CART) and Jeffries Matusita method) successfully selected optimal wavelengths for species discrimination at leaf level. Only 17 out of 2150 wavelengths were identified, thereby reducing the complexities related to data dimensionality. The optimal 17 wavelength bands were noted in the visible (438, 442, 512 and 695 nm), near infrared (724, 729, 750, 758, 856, 936, 1179, 1507 and 1673 nm) and mid-infrared (2220, 2465, 2469 and 2482 nm) portions of the electromagnetic spectrum. The Jeffries-Matusita (JM) distance method confirmed the separability of the selected wavelength bands. Using these 17 wavelengths, linear discriminant analysis (LDA) classified subtropical species at leaf level more accurately than partial least squares discriminant analysis (PLSDA) and random forest (RF). In addition, the study integrated field-collected canopy spectral and multispectral data to discriminate proportions of semi-deciduous and evergreen subtropical forests at sub-pixel level. By using the 2013 land cover (using MLC) to mask non-forested portions before sub-pixel classification (using MTMF), the proportional maps were a product of two classifiers. The proportional maps show higher proportions of evergreen forests along the coast while semi-deciduous subtropical forest species were mainly on inland parts of the Wild Coast. These maps had high accuracy, thereby proving the ability of an integration of field spectral and multispectral data in mapping semi-deciduous and evergreen forest species. Overall, the study has demonstrated the importance of the MLC and LDA and served to integrate field spectral and multispectral data in subtropical forest characterisation at both leaf and top-of-atmosphere levels. The success of both the MLC and LDA further highlighted how essential parametric classifiers are in remote sensing forestry applications. Main subtropical characteristics highlighted in this study were species discrimination at leaf level, quantifying forest change at pixel level and discriminating semi-deciduous and evergreen forests at sub-pixel level.
- Full Text:
- Date Issued: 2015
- Authors: Blessing, Sithole Vhusomuzi
- Date: 2015
- Subjects: Forests and forestry -- South Africa -- Remote sensing , Forest conservation , Remote sensing , Geographic information systems
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10677 , http://hdl.handle.net/10948/d1021169
- Description: The subtropical forests located along South Africa’s Wild Coast region, declared as one of the biodiversity hotspots, provide benefits to the local and national economy. However, there is evidence of increased pressure exerted on the forests by growing population and reduced income from activities not related to forest products. The ability of remote sensing to quantify subtropical forest changes over time, perform species discrimination (using field spectroscopy) and integrating field spectral and multispectral data were all assessed in this study. Investigations were conducted at pixel, leaf and sub-pixel levels. Both per-pixel and sub-pixel classification methods were used for improved forest characterisation. Using SPOT 6 imagery for 2013, the study determined the best classification algorithm for mapping sub-tropical forest and other land cover types to be the maximum likelihood classifier. Maximum likelihood outperformed minimum distance, spectral angle mapper and spectral information divergence algorithms, based on overall accuracy and Kappa coefficient values. Forest change analysis was made based on spectral measurements made at top of the atmosphere (TOC) level. When applied to the 2005 and 2009 SPOT 5 images, subtropical forest changes between 2005-2009 and 2009-2013 were quantified. A temporal analysis of forest cover trends in the periods 2005-2009 and 2009-2013 identified a decreasing trend of -3648.42 and -946.98 ha respectively, which translated to 7.81 percent and 2.20 percent decrease. Although there is evidence of a trend towards decreased rates of forest loss, more conservation efforts are required to protect the Wild Coast ecosystem. Using field spectral measurements data, the hierarchical method (comprising One-way ANOVA with Bonferroni correction, Classification and Regression Trees (CART) and Jeffries Matusita method) successfully selected optimal wavelengths for species discrimination at leaf level. Only 17 out of 2150 wavelengths were identified, thereby reducing the complexities related to data dimensionality. The optimal 17 wavelength bands were noted in the visible (438, 442, 512 and 695 nm), near infrared (724, 729, 750, 758, 856, 936, 1179, 1507 and 1673 nm) and mid-infrared (2220, 2465, 2469 and 2482 nm) portions of the electromagnetic spectrum. The Jeffries-Matusita (JM) distance method confirmed the separability of the selected wavelength bands. Using these 17 wavelengths, linear discriminant analysis (LDA) classified subtropical species at leaf level more accurately than partial least squares discriminant analysis (PLSDA) and random forest (RF). In addition, the study integrated field-collected canopy spectral and multispectral data to discriminate proportions of semi-deciduous and evergreen subtropical forests at sub-pixel level. By using the 2013 land cover (using MLC) to mask non-forested portions before sub-pixel classification (using MTMF), the proportional maps were a product of two classifiers. The proportional maps show higher proportions of evergreen forests along the coast while semi-deciduous subtropical forest species were mainly on inland parts of the Wild Coast. These maps had high accuracy, thereby proving the ability of an integration of field spectral and multispectral data in mapping semi-deciduous and evergreen forest species. Overall, the study has demonstrated the importance of the MLC and LDA and served to integrate field spectral and multispectral data in subtropical forest characterisation at both leaf and top-of-atmosphere levels. The success of both the MLC and LDA further highlighted how essential parametric classifiers are in remote sensing forestry applications. Main subtropical characteristics highlighted in this study were species discrimination at leaf level, quantifying forest change at pixel level and discriminating semi-deciduous and evergreen forests at sub-pixel level.
- Full Text:
- Date Issued: 2015
Application of bacteriocins in the preservation of fruit juice
- Authors: Bodley, Mark David
- Date: 2015
- Subjects: Bacteriocins , Fruit juices -- Preservation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10345 , http://hdl.handle.net/10948/d1020188
- Description: Bacteriocins (BCNs) are ribosomally synthesized polypeptides or proteins with antimicrobial activity, produced by different groups of bacteria. Many lactic acid bacteria (LAB) produce BCNs with broad spectra of inhibition. The antimicrobial activity of BCNs against spoilage organisms (SPOs) has raised considerable interest in their application in juice preservation. The objectives of the study were to: (i) isolate, identify and screen BCN producing bacteria for antimicrobial activity against spoilage bacteria and fungi, (ii) optimize production of BCN from selected producers and (iii) investigate the industrial application of the BCN as a preservative in fruit juice. Eleven LAB strains of BCN producers were screened for antimicrobial activity. BCNs from Lactobacillus plantarum and Pediococcus pentosaceus 34 were the most effective against juice spoilage bacteria and fungi. The effect of medium components on bacteriocin production in L. plantarum and P. pentosaceus 34 was also determined. Clementine:Valencia (1:1) juice was used for the first time as the growth medium for L. plantarum and P. pentosaceus 34. The BCN from L. plantarum showed the highest activity and was, therefore, chosen for juice fermentation studies. The identification of L. plantarum was confirmed by biochemical tests, polymerase chain reaction (PCR) and sequencing of the recA gene. The highest BCN activity was observed for L. plantarum grown in De Man-Rogosa-Sharpe (MRS) and a combination of all supplements (i.e. peptone, MnSO4.H2O, Tween 80, glucose and whey), followed by MRS and Tween 80, peptone, MnSO4.H2O and MRS alone. MRS was a better medium for BCN production than juice [Clementine:Valencia (1:1)]. Size exclusion chromatography (SEC) was used to isolate the active L. plantarum BCN fraction which corresponded to an approximate molecular weight of 3.2 kDa and was proteinaceous in nature. Plantaricin structural genes (plnEF, plnJ, plnK, plnN) were detected in the L. plantarum strain by PCR and sequenced, and were chromosomally encoded as no plasmids could be detected. This implies that the BCN from L. plantarum is most likely a type of class IIa plantaricin which is responsible for the broad inhibitory activity observed. For the industrial application studies, L. plantarum BCN-containing cell free supernatant (BCNsup) added to “Ready to Drink” (RTD) Clementine:Valencia (1:1) juice at concentrations of 3 600 - 500 000 ppm decreased growth of SPOs, Lactobacillus acidophilus and Streptococcus thermophilus. At 250 000 ppm, the L. plantarum BCNsup achieved 5.3 and 6.8 log reductions of the L. acidophilus, after 24 and 48 h, respectively, which is larger than the USFDA (2001) requirement of a 5 log reduction in SPO activity, for preservation of fruit juices. However, there was a decrease in the activity when the BCNsup was applied to industrial (Valor) RTD juice (mango-orange) at decreasing concentrations of 100 000, 50 000 and 25 000 ppm. Organoleptic tests showed that the BCN did not alter flavor or taste of the juice and did not cause toxicity or allergic reactions. A food safety risk assessment was conducted in order to determine the Critical Control Point(s) [CCP(s)] at which the BCN could be applied to control identified microbiological hazards, and a Hazard Analysis and Critical Control Point (HACCP) plan was developed. This is the first report on the optimisation of L. plantarum BCN production in juice [Clementine:Valencia (1:1)], followed by inoculation into RTD juice (mango-orange), including a HACCP plan for the application of the BCN as a preservative in juice.
- Full Text:
- Date Issued: 2015
- Authors: Bodley, Mark David
- Date: 2015
- Subjects: Bacteriocins , Fruit juices -- Preservation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10345 , http://hdl.handle.net/10948/d1020188
- Description: Bacteriocins (BCNs) are ribosomally synthesized polypeptides or proteins with antimicrobial activity, produced by different groups of bacteria. Many lactic acid bacteria (LAB) produce BCNs with broad spectra of inhibition. The antimicrobial activity of BCNs against spoilage organisms (SPOs) has raised considerable interest in their application in juice preservation. The objectives of the study were to: (i) isolate, identify and screen BCN producing bacteria for antimicrobial activity against spoilage bacteria and fungi, (ii) optimize production of BCN from selected producers and (iii) investigate the industrial application of the BCN as a preservative in fruit juice. Eleven LAB strains of BCN producers were screened for antimicrobial activity. BCNs from Lactobacillus plantarum and Pediococcus pentosaceus 34 were the most effective against juice spoilage bacteria and fungi. The effect of medium components on bacteriocin production in L. plantarum and P. pentosaceus 34 was also determined. Clementine:Valencia (1:1) juice was used for the first time as the growth medium for L. plantarum and P. pentosaceus 34. The BCN from L. plantarum showed the highest activity and was, therefore, chosen for juice fermentation studies. The identification of L. plantarum was confirmed by biochemical tests, polymerase chain reaction (PCR) and sequencing of the recA gene. The highest BCN activity was observed for L. plantarum grown in De Man-Rogosa-Sharpe (MRS) and a combination of all supplements (i.e. peptone, MnSO4.H2O, Tween 80, glucose and whey), followed by MRS and Tween 80, peptone, MnSO4.H2O and MRS alone. MRS was a better medium for BCN production than juice [Clementine:Valencia (1:1)]. Size exclusion chromatography (SEC) was used to isolate the active L. plantarum BCN fraction which corresponded to an approximate molecular weight of 3.2 kDa and was proteinaceous in nature. Plantaricin structural genes (plnEF, plnJ, plnK, plnN) were detected in the L. plantarum strain by PCR and sequenced, and were chromosomally encoded as no plasmids could be detected. This implies that the BCN from L. plantarum is most likely a type of class IIa plantaricin which is responsible for the broad inhibitory activity observed. For the industrial application studies, L. plantarum BCN-containing cell free supernatant (BCNsup) added to “Ready to Drink” (RTD) Clementine:Valencia (1:1) juice at concentrations of 3 600 - 500 000 ppm decreased growth of SPOs, Lactobacillus acidophilus and Streptococcus thermophilus. At 250 000 ppm, the L. plantarum BCNsup achieved 5.3 and 6.8 log reductions of the L. acidophilus, after 24 and 48 h, respectively, which is larger than the USFDA (2001) requirement of a 5 log reduction in SPO activity, for preservation of fruit juices. However, there was a decrease in the activity when the BCNsup was applied to industrial (Valor) RTD juice (mango-orange) at decreasing concentrations of 100 000, 50 000 and 25 000 ppm. Organoleptic tests showed that the BCN did not alter flavor or taste of the juice and did not cause toxicity or allergic reactions. A food safety risk assessment was conducted in order to determine the Critical Control Point(s) [CCP(s)] at which the BCN could be applied to control identified microbiological hazards, and a Hazard Analysis and Critical Control Point (HACCP) plan was developed. This is the first report on the optimisation of L. plantarum BCN production in juice [Clementine:Valencia (1:1)], followed by inoculation into RTD juice (mango-orange), including a HACCP plan for the application of the BCN as a preservative in juice.
- Full Text:
- Date Issued: 2015
An evaluation of the potential for implementing adaptive co-management in the Waodani social-ecological system in the Ecuadorian Amazon
- Authors: Bryja, Malgorzata Anna
- Date: 2015
- Subjects: Adaptive natural resource management -- Ecuador -- Reserva de Biosfera Yasuni , Huao Indians -- Ecuador -- Reserva de Biosfera Yasuni , Natural resources -- Co-management -- Ecuador -- Reserva de Biosfera Yasuni , Social ecology -- Ecuador -- Reserva de Biosfera Yasuni , Sustainable development -- Ecuador -- Reserva de Biosfera Yasuni , Resource-based communities -- Ecuador -- Reserva de Biosfera Yasuni , Reserva de Biosfera Yasuni (Ecuador) -- Economic conditions , Huao Indians -- Ecuador -- Reserva de Biosfera Yasuni -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4784 , http://hdl.handle.net/10962/d1018192
- Description: Adaptive co-management (ACM), one of the most prominent management approaches to emerge in the recent years, combines iterative learning, flexibility, and adaptation promoted by adaptive management with the principles of nurturing diversity and fostering collaboration among different partners that underpin co-management philosophy. ACM has been proposed as an approach to address the deficiencies of centralized management in ensuring sustainability of social-ecological systems (SESs) in face of future uncertainties. This thesis aims to evaluate the readiness of resource users (the Waodani) as well as external actors (the Ecuadorian State and NGOs) for future implementation of ACM and thus enhancing the long-term social-ecological sustainability of the Waodani SES located in the Yasuni Biosphere Reserve in the Ecuadorian Amazon. Qualitative data obtained by means of focus groups with the Waodani and individual interviews with external actors and Waodani leaders revealed different levels of readiness for ACM. Firstly, in the case of the Waodani, the insufficient fulfillment of some conditions required for successful ACM as well as intercommunity differences in regards to these conditions can complicate the implementation of ACM, unless sufficient external assistance is offered to the SES. The analysis of NGOs demonstrated, on the other hand, a relative readiness for ACM, providing that such aspects as sufficient funding, long-term commitment to collaboration, and inter-institutional linkages are strengthened. The study also found that the Ecuadorian government’s potential to contribute to ACM is hindered by the lack of readiness to work with the indigenous society as well as by funding and communication challenges. Furthermore, the resource based economy supported by the State limits the scope of innovation and adaptation. Still, as in the case of other actors, overcoming the challenges and transitioning towards adaptive governance and thus ACM could be possible in the long-term, if recent legal and political changes are truly implemented.
- Full Text:
- Date Issued: 2015
- Authors: Bryja, Malgorzata Anna
- Date: 2015
- Subjects: Adaptive natural resource management -- Ecuador -- Reserva de Biosfera Yasuni , Huao Indians -- Ecuador -- Reserva de Biosfera Yasuni , Natural resources -- Co-management -- Ecuador -- Reserva de Biosfera Yasuni , Social ecology -- Ecuador -- Reserva de Biosfera Yasuni , Sustainable development -- Ecuador -- Reserva de Biosfera Yasuni , Resource-based communities -- Ecuador -- Reserva de Biosfera Yasuni , Reserva de Biosfera Yasuni (Ecuador) -- Economic conditions , Huao Indians -- Ecuador -- Reserva de Biosfera Yasuni -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4784 , http://hdl.handle.net/10962/d1018192
- Description: Adaptive co-management (ACM), one of the most prominent management approaches to emerge in the recent years, combines iterative learning, flexibility, and adaptation promoted by adaptive management with the principles of nurturing diversity and fostering collaboration among different partners that underpin co-management philosophy. ACM has been proposed as an approach to address the deficiencies of centralized management in ensuring sustainability of social-ecological systems (SESs) in face of future uncertainties. This thesis aims to evaluate the readiness of resource users (the Waodani) as well as external actors (the Ecuadorian State and NGOs) for future implementation of ACM and thus enhancing the long-term social-ecological sustainability of the Waodani SES located in the Yasuni Biosphere Reserve in the Ecuadorian Amazon. Qualitative data obtained by means of focus groups with the Waodani and individual interviews with external actors and Waodani leaders revealed different levels of readiness for ACM. Firstly, in the case of the Waodani, the insufficient fulfillment of some conditions required for successful ACM as well as intercommunity differences in regards to these conditions can complicate the implementation of ACM, unless sufficient external assistance is offered to the SES. The analysis of NGOs demonstrated, on the other hand, a relative readiness for ACM, providing that such aspects as sufficient funding, long-term commitment to collaboration, and inter-institutional linkages are strengthened. The study also found that the Ecuadorian government’s potential to contribute to ACM is hindered by the lack of readiness to work with the indigenous society as well as by funding and communication challenges. Furthermore, the resource based economy supported by the State limits the scope of innovation and adaptation. Still, as in the case of other actors, overcoming the challenges and transitioning towards adaptive governance and thus ACM could be possible in the long-term, if recent legal and political changes are truly implemented.
- Full Text:
- Date Issued: 2015
Towards a Mobile Bioethanol Unit for point of source conversion of sugar sources to bioethanol: design and feasibility study for South Africa
- Authors: Cech, Alexandra Louise
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/59141 , vital:27439
- Description: Restricted access-thesis embargoed for 5 years
- Full Text:
- Date Issued: 2015
- Authors: Cech, Alexandra Louise
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/59141 , vital:27439
- Description: Restricted access-thesis embargoed for 5 years
- Full Text:
- Date Issued: 2015
Production of Cydia pomonella granulovirus (CpGV) in a heteralogous host, Thaumatotibia Leucotreta (Meyrick) (False codling moth)
- Authors: Chambers, Craig Brian
- Date: 2015
- Subjects: Cryptophlebia leucotreta -- South Africa , Codling moth -- South Africa , Apples -- Diseases and pests -- South Africa , Codling moth -- Biological control -- South Africa , Insect pests -- Biological control -- South Africa , Biological pest control agents -- South Africa , Baculoviruses -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5935 , http://hdl.handle.net/10962/d1017906
- Description: Cydia pomonella (Linnaeus) (Family: Tortricidae), the codling moth, is considered one of the most significant pests of apples and pears worldwide, causing up to 80% crop loss in orchards if no control measures are applied. Cydia pomonella is oligophagous feeding on a number of alternate hosts including quince, walnuts, apricots, peaches, plums and nectarines. Historically the control of this pest has been achieved with the use of various chemical control strategies which have maintained pest levels below the economic threshold at a relatively low cost to the grower. However, there are serious concerns surrounding the use of chemical insecticides including the development of resistance in insect populations, the banning of various insecticides, regulations for lowering of the maximum residue level and employee and consumer safety. For this reason, alternate measures of control are slowly being adopted by growers such as mating disruption, cultural methods and the use of baculovirus biopesticides as part of integrated pest management programmes. The reluctance of growers to accept baculovirus or other biological control products in the past has been due to questionable product quality and inconsistencies in their field performance. Moreover, the development and application of biological control products is more costly than the use of chemical alternatives. Baculoviruses are arthropod specific viruses that are highly virulent to a number of lepidopteran species. Due to the virulence and host specificity of baculoviruses, Cydia pomonella granulovirus has been extensively and successfully used as part of integrated pest management systems for the control of C. pomonella in Europe and around the world, including South Africa. Commercial formulations have been typically based on the Mexican strain of CpGV. However due to long-term multiple applications of CpGV and the reliance on CpGV in organic farming practices in Europe, resistance to the CpGV-M strain has developed in a number of field populations of C. pomonella. This study aimed to identify and characterize novel isolates of CpGV in South Africa and compare their virulence with the commercial standard CpGV-M. Secondly, since C. pomonella is difficult to culture on a large scale, an alternate method of CpGV production was investigated in order to determine if CpGV could be produced more efficiently and at a reduced cost without negatively impacting the quality of the product. Several isolates of CpGV were recovered either from field collected larvae or from a laboratory-reared C. pomonella colony. Characterisation of DNA profiles using a variety of restriction enzymes revealed that only a single isolate, CpGV-SA, was genetically different from the Mexican strain of the virus used in the commercially available CpGV based products in South Africa. In dose-response bioassays using CpGV-SA, LC₅₀ and LC₉₀ values for neonate C. pomonella larvae were 3.18 x 10³ OBs/ml and 7.33 x 10⁴ respectively. A comparison of these values with those of CpGV-M indicated no significant difference in the virulence of the two isolates under laboratory conditions. This is a first report of a genetically distinct CpGV isolate in South Africa. The biological activity and novelty of CpGV-SA makes this isolate a potentially important tool for CpGV resistance management in South Africa. In order to justify production of CpGV in an alternative host, studies on the comparative biological performance of C. pomonella and T. leucotreta based on oviposition, time to hatch, larval developmental times and rearing efficiency as well as production costs were performed. Thaumatotibia leucotreta was found to be more fecund and to have significantly shorter egg and larval developmental times. In addition, larval production per unit of artificial diet was significantly higher than for C. pomonella. This resulted in T. leucotreta being more cost effective to produce with implications for reduced insectary space, sanitation practices as well as the labour component of production. Virus yield data generated by inoculation both C. pomonella and T. leucotreta with nine concentrations of CpGV resulted in comparable virus yields, justifying the continuation of the research into production of CpGV in T. leucotreta. It was important to determine the LC and LT values required for mass production of CpGV in late instar T. leucotreta larvae. Dose- and time-response bioassays with CpGV-M were conducted on artificial diet to determine these values. Fourth instar LC₅₀ and LC₉₀ values were 5.96 x 10³ OBs/ml and 1.64 x 10⁵ OBs/ml respectively. LT50 and LT90 values were 81.10 hours and 88.58 hours respectively. Fifth instar LC₅₀ and LC₉₀ values were 6.88 x 10⁴ OBs/ml and 9.78 x 10⁶ OBs/ml respectively. LT₅₀ and LT₉₀ values were 111.56 hours and 137.57 hours respectively. Virus produced in fourth instar T. leucotreta larvae was bioassayed against C. pomonella neonate larvae and compared to CpGV-M to establish if production in the heterologous host negatively affected the virulence of the isolate. No significant difference in virulence was observed between virus produced in T. leucotreta and that produced in C. pomonella. The data generated in the bioassays was used in CpGV mass production trials to evaluate production. All production methods tested produced acceptable virus yields. To examine the quality of the virus product, genomic DNA was extracted from larval cadavers and subjected to REN analysis with HindIII. The resulting DNA profiles indicated that the virus product was contaminated with the homologous virus, CrleGV. Based on the above results, the use of T. leucotreta as an alternate host for the in vivo production of CpGV on a commercial basis is not at this stage viable and requires further investigation before this production methodology can be reliable used to produce CpGV. However, this study has shown that CpGV can be produced in a homologous host, T. leucotreta and significant strides have been made towards developing a set of quality control standards that are essential for further development of successful production methodology. Finally a novel isolate of CpGV has been identified with comparable virulence to the CpGV-M. This is an important finding as it has broad reaching implications for resistance management of CpGV products in South Africa.
- Full Text:
- Date Issued: 2015
- Authors: Chambers, Craig Brian
- Date: 2015
- Subjects: Cryptophlebia leucotreta -- South Africa , Codling moth -- South Africa , Apples -- Diseases and pests -- South Africa , Codling moth -- Biological control -- South Africa , Insect pests -- Biological control -- South Africa , Biological pest control agents -- South Africa , Baculoviruses -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5935 , http://hdl.handle.net/10962/d1017906
- Description: Cydia pomonella (Linnaeus) (Family: Tortricidae), the codling moth, is considered one of the most significant pests of apples and pears worldwide, causing up to 80% crop loss in orchards if no control measures are applied. Cydia pomonella is oligophagous feeding on a number of alternate hosts including quince, walnuts, apricots, peaches, plums and nectarines. Historically the control of this pest has been achieved with the use of various chemical control strategies which have maintained pest levels below the economic threshold at a relatively low cost to the grower. However, there are serious concerns surrounding the use of chemical insecticides including the development of resistance in insect populations, the banning of various insecticides, regulations for lowering of the maximum residue level and employee and consumer safety. For this reason, alternate measures of control are slowly being adopted by growers such as mating disruption, cultural methods and the use of baculovirus biopesticides as part of integrated pest management programmes. The reluctance of growers to accept baculovirus or other biological control products in the past has been due to questionable product quality and inconsistencies in their field performance. Moreover, the development and application of biological control products is more costly than the use of chemical alternatives. Baculoviruses are arthropod specific viruses that are highly virulent to a number of lepidopteran species. Due to the virulence and host specificity of baculoviruses, Cydia pomonella granulovirus has been extensively and successfully used as part of integrated pest management systems for the control of C. pomonella in Europe and around the world, including South Africa. Commercial formulations have been typically based on the Mexican strain of CpGV. However due to long-term multiple applications of CpGV and the reliance on CpGV in organic farming practices in Europe, resistance to the CpGV-M strain has developed in a number of field populations of C. pomonella. This study aimed to identify and characterize novel isolates of CpGV in South Africa and compare their virulence with the commercial standard CpGV-M. Secondly, since C. pomonella is difficult to culture on a large scale, an alternate method of CpGV production was investigated in order to determine if CpGV could be produced more efficiently and at a reduced cost without negatively impacting the quality of the product. Several isolates of CpGV were recovered either from field collected larvae or from a laboratory-reared C. pomonella colony. Characterisation of DNA profiles using a variety of restriction enzymes revealed that only a single isolate, CpGV-SA, was genetically different from the Mexican strain of the virus used in the commercially available CpGV based products in South Africa. In dose-response bioassays using CpGV-SA, LC₅₀ and LC₉₀ values for neonate C. pomonella larvae were 3.18 x 10³ OBs/ml and 7.33 x 10⁴ respectively. A comparison of these values with those of CpGV-M indicated no significant difference in the virulence of the two isolates under laboratory conditions. This is a first report of a genetically distinct CpGV isolate in South Africa. The biological activity and novelty of CpGV-SA makes this isolate a potentially important tool for CpGV resistance management in South Africa. In order to justify production of CpGV in an alternative host, studies on the comparative biological performance of C. pomonella and T. leucotreta based on oviposition, time to hatch, larval developmental times and rearing efficiency as well as production costs were performed. Thaumatotibia leucotreta was found to be more fecund and to have significantly shorter egg and larval developmental times. In addition, larval production per unit of artificial diet was significantly higher than for C. pomonella. This resulted in T. leucotreta being more cost effective to produce with implications for reduced insectary space, sanitation practices as well as the labour component of production. Virus yield data generated by inoculation both C. pomonella and T. leucotreta with nine concentrations of CpGV resulted in comparable virus yields, justifying the continuation of the research into production of CpGV in T. leucotreta. It was important to determine the LC and LT values required for mass production of CpGV in late instar T. leucotreta larvae. Dose- and time-response bioassays with CpGV-M were conducted on artificial diet to determine these values. Fourth instar LC₅₀ and LC₉₀ values were 5.96 x 10³ OBs/ml and 1.64 x 10⁵ OBs/ml respectively. LT50 and LT90 values were 81.10 hours and 88.58 hours respectively. Fifth instar LC₅₀ and LC₉₀ values were 6.88 x 10⁴ OBs/ml and 9.78 x 10⁶ OBs/ml respectively. LT₅₀ and LT₉₀ values were 111.56 hours and 137.57 hours respectively. Virus produced in fourth instar T. leucotreta larvae was bioassayed against C. pomonella neonate larvae and compared to CpGV-M to establish if production in the heterologous host negatively affected the virulence of the isolate. No significant difference in virulence was observed between virus produced in T. leucotreta and that produced in C. pomonella. The data generated in the bioassays was used in CpGV mass production trials to evaluate production. All production methods tested produced acceptable virus yields. To examine the quality of the virus product, genomic DNA was extracted from larval cadavers and subjected to REN analysis with HindIII. The resulting DNA profiles indicated that the virus product was contaminated with the homologous virus, CrleGV. Based on the above results, the use of T. leucotreta as an alternate host for the in vivo production of CpGV on a commercial basis is not at this stage viable and requires further investigation before this production methodology can be reliable used to produce CpGV. However, this study has shown that CpGV can be produced in a homologous host, T. leucotreta and significant strides have been made towards developing a set of quality control standards that are essential for further development of successful production methodology. Finally a novel isolate of CpGV has been identified with comparable virulence to the CpGV-M. This is an important finding as it has broad reaching implications for resistance management of CpGV products in South Africa.
- Full Text:
- Date Issued: 2015
Towards a changing context and performance practice of mbira dzavadzimu music in Zimbabwe
- Authors: Chipendo, Claudio
- Date: 2015
- Subjects: Mbira music -- Zimbabwe Shona (African people) -- Music
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/6357 , vital:29588
- Description: Mbira dzavadzimu music and performance practice has been in existence since the pre-colonial era. It played a crucial role in ritual and non-ritual activities of the Shona people of Zimbabwe. However, political, social and global influences as well as technological advancement have resulted in change of context and performance practice. Unfortunately, these have not been recorded for future generations. The major aim of the study is therefore to examine the change of context and performance practice of mbira dzavadzimu in Zimbabwe. This was achieved by reviewing mbira dzavadzimu music and performance practice within the modern setting of dandaro. I looked at change from a theoretical lens of the theory of diffusion, syncretism and mediatisation. The study was in the qualitative form superimposed on some case studies. Unstructured interviews, participant and non-participant observations were the main instruments used to collect data from both traditional and modern mbira performances. Data was also collected from museums, archives, radio and television stations. The study established that the changes in context and performance practice of mbira dzavadzimu in Zimbabwe were to a larger extent due to foreign influences such as colonialism, the coming of missionaries, modernisation, urbanisation, commercialisation, mediatisation, the use of modern technology and institutionalisation. Due to the aforementioned influences, the environmental settings, the change of context from sacred to secular, the relationship with ancestral spirits, musical practices, performance situations and quality of sound, have been modified and adjusted in response to the influences of the globalised world’s ever changing audience and performance space. In short, this has resulted in a shift of mbira performances from its traditional to modern settings, from the village to the city and onto the international scene with a new performer-audience setting. Various innovations were carried out on the instrument and its music as a result of the advent of modern technology. The use of microphones, modern amplification systems, recording studios, radio and television broadcast, audio and video cassettes, CDs, DVD, teaching of the instrument using audio and video instructional models and the use of internet sites in learning how to play mbira dzavadzimu and other instruments have become a reality. It has been evident from the study that urban and rural areas take up change in different ways and that in the former change is more pronounced than in the latter. Urban area communities are more “developed” than their rural counterparts because the former are more exposed to technological influences and the commercialisation of music. The study has also established that Zimbabwean mbira music is a good example of modern transculturality. The instrument and its music have played a major role in breaking down cultural boundaries and bringing the people of the world together for purposes of performing on the instrument. From the findings of this study, I attribute most of the changes to technologisation, for most of the changes that have taken place on mbira dzavadzimu were a result of the highly technologised way of life Zimbabweans now lead.
- Full Text:
- Date Issued: 2015
- Authors: Chipendo, Claudio
- Date: 2015
- Subjects: Mbira music -- Zimbabwe Shona (African people) -- Music
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/6357 , vital:29588
- Description: Mbira dzavadzimu music and performance practice has been in existence since the pre-colonial era. It played a crucial role in ritual and non-ritual activities of the Shona people of Zimbabwe. However, political, social and global influences as well as technological advancement have resulted in change of context and performance practice. Unfortunately, these have not been recorded for future generations. The major aim of the study is therefore to examine the change of context and performance practice of mbira dzavadzimu in Zimbabwe. This was achieved by reviewing mbira dzavadzimu music and performance practice within the modern setting of dandaro. I looked at change from a theoretical lens of the theory of diffusion, syncretism and mediatisation. The study was in the qualitative form superimposed on some case studies. Unstructured interviews, participant and non-participant observations were the main instruments used to collect data from both traditional and modern mbira performances. Data was also collected from museums, archives, radio and television stations. The study established that the changes in context and performance practice of mbira dzavadzimu in Zimbabwe were to a larger extent due to foreign influences such as colonialism, the coming of missionaries, modernisation, urbanisation, commercialisation, mediatisation, the use of modern technology and institutionalisation. Due to the aforementioned influences, the environmental settings, the change of context from sacred to secular, the relationship with ancestral spirits, musical practices, performance situations and quality of sound, have been modified and adjusted in response to the influences of the globalised world’s ever changing audience and performance space. In short, this has resulted in a shift of mbira performances from its traditional to modern settings, from the village to the city and onto the international scene with a new performer-audience setting. Various innovations were carried out on the instrument and its music as a result of the advent of modern technology. The use of microphones, modern amplification systems, recording studios, radio and television broadcast, audio and video cassettes, CDs, DVD, teaching of the instrument using audio and video instructional models and the use of internet sites in learning how to play mbira dzavadzimu and other instruments have become a reality. It has been evident from the study that urban and rural areas take up change in different ways and that in the former change is more pronounced than in the latter. Urban area communities are more “developed” than their rural counterparts because the former are more exposed to technological influences and the commercialisation of music. The study has also established that Zimbabwean mbira music is a good example of modern transculturality. The instrument and its music have played a major role in breaking down cultural boundaries and bringing the people of the world together for purposes of performing on the instrument. From the findings of this study, I attribute most of the changes to technologisation, for most of the changes that have taken place on mbira dzavadzimu were a result of the highly technologised way of life Zimbabweans now lead.
- Full Text:
- Date Issued: 2015
Synthesis and biological evaluation of truncated sarganaphthoquinoic acid derivatives as Hsp90 inhibitors
- Authors: Chiwakata, Maynard T
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64708 , vital:28594
- Description: Hsp90 inhibition has been at the centre of attention in current research due to the possibility of “cracking down” on the entire process leading to the development of malignant cancers. Small underlying principles common in all types of cancers have been determined that govern the transformation of normal human cells into cancerous cells, with all relying on the ATPase activity of Hsp90 protein. Hsp90 protein is therefore an attractive drug target that if successfully inhibited can result in the remission of cancer tumours by one form of treatment. To date, no Hsp90 inhibitor has been sanctioned for cancer treatment as most are still in clinical development. Our research was therefore inspired by reports that indicated the potential of quinones / naphthoquinones to act as Hsp90 inhibitors. Preliminary results of a few selected marine natural product quinone systems i.e. sargaquinoic acid (SQA) (2.47) and lapachol (3.6) showed moderate cytotoxicity and weak interactions with the Hsp90 molecular chaperone, and evidence suggested C-terminal binding of these molecules. No correlation has been determined yet between cytotoxicity and Hsp90 inhibition, hence we aimed to develop natural product inspired molecules that exhibit both cytotoxic and Hsp90 inhibition properties. Due to limited amounts of the natural product that can be acquired from natural sources, synthetic analogues were opted for. Isolation of a few selected quinones was conducted to have material that could be used in biological assays. For structural modifications, a series of truncated naphthoquinone systems were prepared adopting the sarganaphthoquinoic acid (3.5) scaffold. The naphthoquinones were prepared via Diels-Alder reactions of relevant benzoquinones with myrcene, followed by aromatization reactions using MnO2. Various alkyl and aryl amines were then coupled to the C-2/3 position of the naphthoquinone using Michael’s addition reactions. Tricyclic naphthoquinones were also synthesized from reactions with hypotaurine and citral. Design of the analogues incorporated functionalities from known Hsp90 inhibitors e.g. geldanamycin (2.28) and its analogues. Preliminary results obtained showed that coupling of naphthoquinones with aryl-amines resulted in the most cytotoxic compounds (4.14-4.19) with IC50 values as low as 0.3 μM against Hs578T breast cancer carcinoma (triple negative). Most of the alkyl amines (4.20-4.25) had IC50 values greater than 50 μM except for 4.20 and 4.21 that showed IC50 values of 7.6 μM and 2.6 μM respectively. Tricyclic naphthoquinones (4.28-4.29) showed moderate cytotoxic activity of approximately 10 μM. Hsp90 inhibition was assessed by client protein degradation assays, of which SQA (2.47), showed the best Hsp90 inhibition properties, followed by compound 4.20. The most cytotoxic arylamino-naphthoquinone (4.16) and tricyclic naphthoquinones (4.28-4.29) showed only moderate inhibition. None of the compounds led to Hsp70 induction, suggesting possible binding to the C-terminus of Hsp90. Interactions at the binding site were assessed by molecular docking studies and saturation transfer difference (STD) NMR. Docking studies were conducted on the N-terminus of Hsp90 and better binding was observed for arylamino naphthoquinones (4.14-4.19) than for other series of compounds. Unfortunately, the co-crystal structure for the C-terminus of Hsp90 is unavailable, hence docking study comparisons on both domains could not be conducted. However, STD NMR offered a platform to assess binding interactions between the naphthoquinones and the N- or C-terminal domains of Hsp90. However no interactions were observed at both the N- and C- termini of Hsp90 due to either weak binding of ligands to the protein or poor water solubility of the ligands. From these preliminary results, naphthoquinones bind to Hsp90 protein but conclusive remarks to which terminal domain they bind to could not be made. The best candidate from amongst the series of naphthoquinones prepared that showed moderate cytotoxicity and promising Hsp90 inhibition was compound 4.20. We therefore succeeded in developing a new series of naphthoquinones that possess moderate cytotoxicity and show Hsp90 inhibition.
- Full Text:
- Date Issued: 2015
- Authors: Chiwakata, Maynard T
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64708 , vital:28594
- Description: Hsp90 inhibition has been at the centre of attention in current research due to the possibility of “cracking down” on the entire process leading to the development of malignant cancers. Small underlying principles common in all types of cancers have been determined that govern the transformation of normal human cells into cancerous cells, with all relying on the ATPase activity of Hsp90 protein. Hsp90 protein is therefore an attractive drug target that if successfully inhibited can result in the remission of cancer tumours by one form of treatment. To date, no Hsp90 inhibitor has been sanctioned for cancer treatment as most are still in clinical development. Our research was therefore inspired by reports that indicated the potential of quinones / naphthoquinones to act as Hsp90 inhibitors. Preliminary results of a few selected marine natural product quinone systems i.e. sargaquinoic acid (SQA) (2.47) and lapachol (3.6) showed moderate cytotoxicity and weak interactions with the Hsp90 molecular chaperone, and evidence suggested C-terminal binding of these molecules. No correlation has been determined yet between cytotoxicity and Hsp90 inhibition, hence we aimed to develop natural product inspired molecules that exhibit both cytotoxic and Hsp90 inhibition properties. Due to limited amounts of the natural product that can be acquired from natural sources, synthetic analogues were opted for. Isolation of a few selected quinones was conducted to have material that could be used in biological assays. For structural modifications, a series of truncated naphthoquinone systems were prepared adopting the sarganaphthoquinoic acid (3.5) scaffold. The naphthoquinones were prepared via Diels-Alder reactions of relevant benzoquinones with myrcene, followed by aromatization reactions using MnO2. Various alkyl and aryl amines were then coupled to the C-2/3 position of the naphthoquinone using Michael’s addition reactions. Tricyclic naphthoquinones were also synthesized from reactions with hypotaurine and citral. Design of the analogues incorporated functionalities from known Hsp90 inhibitors e.g. geldanamycin (2.28) and its analogues. Preliminary results obtained showed that coupling of naphthoquinones with aryl-amines resulted in the most cytotoxic compounds (4.14-4.19) with IC50 values as low as 0.3 μM against Hs578T breast cancer carcinoma (triple negative). Most of the alkyl amines (4.20-4.25) had IC50 values greater than 50 μM except for 4.20 and 4.21 that showed IC50 values of 7.6 μM and 2.6 μM respectively. Tricyclic naphthoquinones (4.28-4.29) showed moderate cytotoxic activity of approximately 10 μM. Hsp90 inhibition was assessed by client protein degradation assays, of which SQA (2.47), showed the best Hsp90 inhibition properties, followed by compound 4.20. The most cytotoxic arylamino-naphthoquinone (4.16) and tricyclic naphthoquinones (4.28-4.29) showed only moderate inhibition. None of the compounds led to Hsp70 induction, suggesting possible binding to the C-terminus of Hsp90. Interactions at the binding site were assessed by molecular docking studies and saturation transfer difference (STD) NMR. Docking studies were conducted on the N-terminus of Hsp90 and better binding was observed for arylamino naphthoquinones (4.14-4.19) than for other series of compounds. Unfortunately, the co-crystal structure for the C-terminus of Hsp90 is unavailable, hence docking study comparisons on both domains could not be conducted. However, STD NMR offered a platform to assess binding interactions between the naphthoquinones and the N- or C-terminal domains of Hsp90. However no interactions were observed at both the N- and C- termini of Hsp90 due to either weak binding of ligands to the protein or poor water solubility of the ligands. From these preliminary results, naphthoquinones bind to Hsp90 protein but conclusive remarks to which terminal domain they bind to could not be made. The best candidate from amongst the series of naphthoquinones prepared that showed moderate cytotoxicity and promising Hsp90 inhibition was compound 4.20. We therefore succeeded in developing a new series of naphthoquinones that possess moderate cytotoxicity and show Hsp90 inhibition.
- Full Text:
- Date Issued: 2015