Radio observation of the Gum Nebula Region
- Authors: Woermann, Beate
- Date: 1997
- Subjects: Radio sources (Astronomy) Nebulae -- Observations Radio astronomy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5485 , http://hdl.handle.net/10962/d1005271
- Description: This thesis describes the results of an investigation of the physical properties of the Gum Nebula. For this investigation a radio continuum map of the region was made at 2326 MHz and resolution ⅓° with the HartRAO antenna. This map was used to generate spectral index images and an infrared to radio flux density ratio (IRR) image. The latter image shows that the IRR of the nebula is in the range 20 to 250, identifying it as an old SNR. Several spectral index images of this region were generated using two different methods, one based on the isolation of the nebula from its background radiation, the other based on TT-plots (Turtle et al., 1962). The two methods yield similar results, which show that the nebula has a thermal shell with a non-thermal region in its interior. Below the galactic plane the thermal region dominates and above the plane the nonthermal region. These results suggest a model of an old SNR with an H II region shell. Spectral line observations of hydrogen recombination lines and hydroxyl (OH) were made with the HartRAO and the Mopra telescopes. The detection of hydrogen recombination lines at four positions in the thermal regions of the nebula give electron temperatures and emission measures in the ranges 4000 to 6000 K and 220 to 460 pc.cm⁻⁶ respectively. The turbulent velocities are of the order of 20 km/s. A search for shocked OH lines at 1667 MHz and 1720 MHz in the Gum Nebula gave results that were negative, but numerous unshocked 1667 MHz OH lines were detected. The latter were used in a test for an expansion of the nebula. The most plausible fit to the data gives an expansion centre at l = 260.5°, b = -2.5° and at a distance of 0.7 kpc from us. The front face angular radius and expansion velocity are 10.5° and 16 km/s respectively. The back face angular radius and expansion velocity are 8.50 and 7 km/s respectively.
- Full Text:
- Authors: Woermann, Beate
- Date: 1997
- Subjects: Radio sources (Astronomy) Nebulae -- Observations Radio astronomy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5485 , http://hdl.handle.net/10962/d1005271
- Description: This thesis describes the results of an investigation of the physical properties of the Gum Nebula. For this investigation a radio continuum map of the region was made at 2326 MHz and resolution ⅓° with the HartRAO antenna. This map was used to generate spectral index images and an infrared to radio flux density ratio (IRR) image. The latter image shows that the IRR of the nebula is in the range 20 to 250, identifying it as an old SNR. Several spectral index images of this region were generated using two different methods, one based on the isolation of the nebula from its background radiation, the other based on TT-plots (Turtle et al., 1962). The two methods yield similar results, which show that the nebula has a thermal shell with a non-thermal region in its interior. Below the galactic plane the thermal region dominates and above the plane the nonthermal region. These results suggest a model of an old SNR with an H II region shell. Spectral line observations of hydrogen recombination lines and hydroxyl (OH) were made with the HartRAO and the Mopra telescopes. The detection of hydrogen recombination lines at four positions in the thermal regions of the nebula give electron temperatures and emission measures in the ranges 4000 to 6000 K and 220 to 460 pc.cm⁻⁶ respectively. The turbulent velocities are of the order of 20 km/s. A search for shocked OH lines at 1667 MHz and 1720 MHz in the Gum Nebula gave results that were negative, but numerous unshocked 1667 MHz OH lines were detected. The latter were used in a test for an expansion of the nebula. The most plausible fit to the data gives an expansion centre at l = 260.5°, b = -2.5° and at a distance of 0.7 kpc from us. The front face angular radius and expansion velocity are 10.5° and 16 km/s respectively. The back face angular radius and expansion velocity are 8.50 and 7 km/s respectively.
- Full Text:
Urban agriculture: advocacy and practice: a discursive study with particular reference to three Eastern Cape centres
- Authors: Webb, Nigel Leigh
- Date: 1997
- Subjects: Urban agriculture -- South Africa -- Eastern Cape Land use, Urban -- South Africa -- Eastern Cape Agriculture -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2095 , http://hdl.handle.net/10962/d1002658
- Description: The purpose of this thesis is to explain the continued advocacy of 'urban agriculture' (UA) in the literature in the face of the seemingly modest role played by cultivation in practice. The analytical framework adopted isrthat of discourse, the theoretical underpinnings of which are derived from the early works of Foucault, and applications such as those of Escobar (1989; 1991) and Ferguson (1990). Using a discursive orientation involves two main tasks - an explanation of how 'UA' has gained some prominence and the man~er in which its currency is able to be maintained. The investigation included an in-depth analysis of the literature and empirical research in th!"e~Eastern Cape centres. The function of the empirical findings is to expose the discourse, as well as to extend the empirical base relating to 'urban agricultural' research in general. The thesis suggests that the growth in interest in 'UA' is a result of a Foucaultian "gap" opening in the discourse. Changes in the conceptualisation of development, the rise-in prominence of the urban poor and emerging ecodevelopment views, among others, have given proponents of "UA' greater room for manoeuvre. However, most case studies promote 'UA' despite providing little evidence of its role in household welfare. The way the discourse maintains its' currency is in the manner in which the objects of 'UA' are constituted. Firstly, the people are characterised as poor, largely divorced from their rural origins, and mainly female. Each of these assertions is questioned on the basis of the literature itself and the empirical findings. Secondly, in the practice of cultivation, emphasis is placed on the inputs used, and the crops that are produced. The treatment of both the inputs and the crops by the literature is cursory. Investigations show that land availability, garden size and irrigation water are e~pecially problematic and vegetable consumption remains low. Four types of alleged benefit associated with 'UA' are analysed - environmental and ecological, psychological and social, financial, and nutritional. In each case commonly held assumptions are undermined by closer investigation. Thus, the thesis contends that the role played by cultivation is a modest one. Most of the claims made on behalf of 'UA' are more congruent with development discourse than actual cultivation practice.
- Full Text:
- Authors: Webb, Nigel Leigh
- Date: 1997
- Subjects: Urban agriculture -- South Africa -- Eastern Cape Land use, Urban -- South Africa -- Eastern Cape Agriculture -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2095 , http://hdl.handle.net/10962/d1002658
- Description: The purpose of this thesis is to explain the continued advocacy of 'urban agriculture' (UA) in the literature in the face of the seemingly modest role played by cultivation in practice. The analytical framework adopted isrthat of discourse, the theoretical underpinnings of which are derived from the early works of Foucault, and applications such as those of Escobar (1989; 1991) and Ferguson (1990). Using a discursive orientation involves two main tasks - an explanation of how 'UA' has gained some prominence and the man~er in which its currency is able to be maintained. The investigation included an in-depth analysis of the literature and empirical research in th!"e~Eastern Cape centres. The function of the empirical findings is to expose the discourse, as well as to extend the empirical base relating to 'urban agricultural' research in general. The thesis suggests that the growth in interest in 'UA' is a result of a Foucaultian "gap" opening in the discourse. Changes in the conceptualisation of development, the rise-in prominence of the urban poor and emerging ecodevelopment views, among others, have given proponents of "UA' greater room for manoeuvre. However, most case studies promote 'UA' despite providing little evidence of its role in household welfare. The way the discourse maintains its' currency is in the manner in which the objects of 'UA' are constituted. Firstly, the people are characterised as poor, largely divorced from their rural origins, and mainly female. Each of these assertions is questioned on the basis of the literature itself and the empirical findings. Secondly, in the practice of cultivation, emphasis is placed on the inputs used, and the crops that are produced. The treatment of both the inputs and the crops by the literature is cursory. Investigations show that land availability, garden size and irrigation water are e~pecially problematic and vegetable consumption remains low. Four types of alleged benefit associated with 'UA' are analysed - environmental and ecological, psychological and social, financial, and nutritional. In each case commonly held assumptions are undermined by closer investigation. Thus, the thesis contends that the role played by cultivation is a modest one. Most of the claims made on behalf of 'UA' are more congruent with development discourse than actual cultivation practice.
- Full Text:
Regulation of tryptophan-2,3-dioxygenase and pineal indoleamines by selected tryptophan derivatives and antidepressants
- Authors: Walsh, Harold Archibold
- Date: 1997
- Subjects: Antidepressants Tryptophan -- Physiological effect Tryptophan -- Therapeutic use Depression, Mental -- Treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4017 , http://hdl.handle.net/10962/d1004077
- Description: The regulation of tryptophan-2,3-dioxygenase (TDO) (EC 1.13.1.12) and, to a lesser extent, pineal indoleamines, both in vitro and in vivo, is examined in this study. Rat liver TDO is a cytosolic enzyme which plays a crucial role in the regulation of circulating tryptophan (TRP) levels. Stimulation of this enzyme by heme enhances the catabolism of TRP, making less TRP available for uptake into the brain and other tissues, and for protein synthesis. At pH 7, the enzyme has an approximate Km of 100μM, is subject to substrate inhibition immediately beyond Sopt([S] at Vmax), and response of the enzyme is cooperative in both uninhibited and inhibited regions. Hill analysis of the uninhibited region reveals a biphasic plot and two classes of binding sites. Negative cooperativity is brought about through deprotonation of the enzyme. Substrate iphibition also occurs at both acidic and basic pH values with concomitant shifts in Sopt. The results obtained indicate that substrate inhibition could be an additional mechanism whereby the flux through the TRP-kynurenine pathway is regulated. TDO is subject to a diurnal rhythm, with peak activity during the pre-dark period and the loweSt activity towards the end of the dark period. It is possible that the enzyme controls the synthesis of the neurotransmitter serotonin (5-HT), and that the circadian rhythm in TDO activity is due to the endogenous rhythm of melatonin (aMT) production by the pineal gland. In the present study, aMT displaces TRP from bovine serum albumin (BSA) in vitro, and it is therefore possible for the indoleamine to regulate the availability of TRP for uptake into the brain for conversion to its derivatives. Chronic intraperitoneal administration of aMT affects physiological hepatic parameters in rats, such as TDO activity and stromal fatty acid composition, whilst no observable effect is demonstrable with respect to protein synthesis, nucleic acid metabolism, membrane fatty acid composition and pineal indole biosynthesis. On the other hand, chronic treatment of rats with antidepressants, the tricyclic desmethylimipramine (DMI) and the selective serotonin reuptake inhibitor (SSRI), fluoxetine, reveals significant negative alterations in TDO concentrations and pineal indole amine synthesis. Combining aMT with any of these two drugs normalises the activity of the hepatic enzyme. DMI is found to be an effective inhibitor of TDO in the micromolar range in vitro, and also affects total enzyme concentrations in vivo. Fluoxetine has no effect on TDO in vitro, but in vivo also reduces total enzyme levels in the liver. However, the SSRI does not affect conjugation between apo- and holoenzyme. Instead, it decreases extant holoenzyme levels. Indoleamine synthesis by the pineal gland, in organ culture, is altered by both antidepressants, although in different ways. DMI increases N-acetylserotonin levels and reduces the output of methoxyindole acetic acid and meth6xytryptophol. Fluoxetine treatment markedly reduces aMT concentrations and also brings about high levels of the 5-HT catabolites, 5-hydroxytryptophol and 5-hydroxyindole acetic acid. Insulin also lowers aMT synthesis significantly in pineal organ cultures, via a mechamsm that involves inhibition of the enzyme, N-acetyl transferase, that regulates aMT synthesis. The effects of insulin on pineal indole metabolism are due to the observation that a carbohydrate rich diet which induces insulin release elevates plasma TRP and brain 5-HT, but has no effect on pineal TRP and indole amine synthesis. It could thus be possible for insulin to have an effect on the pineal, since the latter is outside the blood brain barrier. The finilings of this study support the biogenic amine deficiency hypothesis, implicating some of the major biogenic amines such as noradrenaline (NA), 5-HT and aMT in depression. There is believed to be a deficiency of NA and 5-HT at their respective synapses in the depressed state. The drug DMI could act, firstly, by inhibiting TDO and thus increasing plasma TRP levels, and could, secondly, stimulate NA release and inhibit NA reuptake at the pineal membrane. The combined effect would be to enhance aMT synthesis, with eventual remission. Fluoxetine, on the other hand, appears to utilize a slightly different mode of action to DMI, which seems to focus on the preservation of 5-HT. The fact that aMT counteracts the effects of both antidepressants, and restores the activity of TDO to that of the controls, is also consistent with the observation that the therapeutic action of drugs such as these coincides willi the restoration of normal plasma levels of the neurohormone in depressives. In view of the biogenic amine deficiency hypothesis of depression and the contentious claim that TDO is the major peripheral determinant of brain TRP, brain 5-HT and ultimately aMT, the regulation of TDO is investigated and discussed. The study concludes that TDO activity is regulated by a number of endogenous compounds which are mainly derivatives of TRP, such as aMT and oxidized nicotinamide adenine dinucleotide and exogenous substances, of which DMI and fluoxetine are but two. In addition, modulation of IDO activity in depression appears to be an important aspect of antidepressant action. aMT, the product of the pineal gland, also has the potential to increase plasma TRP and hence forebrain TRP levels, and ultimately 5-HT concentrations, firstly by displacing TRP from serum albumin and secondly by inhibiting TDO.
- Full Text:
- Authors: Walsh, Harold Archibold
- Date: 1997
- Subjects: Antidepressants Tryptophan -- Physiological effect Tryptophan -- Therapeutic use Depression, Mental -- Treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4017 , http://hdl.handle.net/10962/d1004077
- Description: The regulation of tryptophan-2,3-dioxygenase (TDO) (EC 1.13.1.12) and, to a lesser extent, pineal indoleamines, both in vitro and in vivo, is examined in this study. Rat liver TDO is a cytosolic enzyme which plays a crucial role in the regulation of circulating tryptophan (TRP) levels. Stimulation of this enzyme by heme enhances the catabolism of TRP, making less TRP available for uptake into the brain and other tissues, and for protein synthesis. At pH 7, the enzyme has an approximate Km of 100μM, is subject to substrate inhibition immediately beyond Sopt([S] at Vmax), and response of the enzyme is cooperative in both uninhibited and inhibited regions. Hill analysis of the uninhibited region reveals a biphasic plot and two classes of binding sites. Negative cooperativity is brought about through deprotonation of the enzyme. Substrate iphibition also occurs at both acidic and basic pH values with concomitant shifts in Sopt. The results obtained indicate that substrate inhibition could be an additional mechanism whereby the flux through the TRP-kynurenine pathway is regulated. TDO is subject to a diurnal rhythm, with peak activity during the pre-dark period and the loweSt activity towards the end of the dark period. It is possible that the enzyme controls the synthesis of the neurotransmitter serotonin (5-HT), and that the circadian rhythm in TDO activity is due to the endogenous rhythm of melatonin (aMT) production by the pineal gland. In the present study, aMT displaces TRP from bovine serum albumin (BSA) in vitro, and it is therefore possible for the indoleamine to regulate the availability of TRP for uptake into the brain for conversion to its derivatives. Chronic intraperitoneal administration of aMT affects physiological hepatic parameters in rats, such as TDO activity and stromal fatty acid composition, whilst no observable effect is demonstrable with respect to protein synthesis, nucleic acid metabolism, membrane fatty acid composition and pineal indole biosynthesis. On the other hand, chronic treatment of rats with antidepressants, the tricyclic desmethylimipramine (DMI) and the selective serotonin reuptake inhibitor (SSRI), fluoxetine, reveals significant negative alterations in TDO concentrations and pineal indole amine synthesis. Combining aMT with any of these two drugs normalises the activity of the hepatic enzyme. DMI is found to be an effective inhibitor of TDO in the micromolar range in vitro, and also affects total enzyme concentrations in vivo. Fluoxetine has no effect on TDO in vitro, but in vivo also reduces total enzyme levels in the liver. However, the SSRI does not affect conjugation between apo- and holoenzyme. Instead, it decreases extant holoenzyme levels. Indoleamine synthesis by the pineal gland, in organ culture, is altered by both antidepressants, although in different ways. DMI increases N-acetylserotonin levels and reduces the output of methoxyindole acetic acid and meth6xytryptophol. Fluoxetine treatment markedly reduces aMT concentrations and also brings about high levels of the 5-HT catabolites, 5-hydroxytryptophol and 5-hydroxyindole acetic acid. Insulin also lowers aMT synthesis significantly in pineal organ cultures, via a mechamsm that involves inhibition of the enzyme, N-acetyl transferase, that regulates aMT synthesis. The effects of insulin on pineal indole metabolism are due to the observation that a carbohydrate rich diet which induces insulin release elevates plasma TRP and brain 5-HT, but has no effect on pineal TRP and indole amine synthesis. It could thus be possible for insulin to have an effect on the pineal, since the latter is outside the blood brain barrier. The finilings of this study support the biogenic amine deficiency hypothesis, implicating some of the major biogenic amines such as noradrenaline (NA), 5-HT and aMT in depression. There is believed to be a deficiency of NA and 5-HT at their respective synapses in the depressed state. The drug DMI could act, firstly, by inhibiting TDO and thus increasing plasma TRP levels, and could, secondly, stimulate NA release and inhibit NA reuptake at the pineal membrane. The combined effect would be to enhance aMT synthesis, with eventual remission. Fluoxetine, on the other hand, appears to utilize a slightly different mode of action to DMI, which seems to focus on the preservation of 5-HT. The fact that aMT counteracts the effects of both antidepressants, and restores the activity of TDO to that of the controls, is also consistent with the observation that the therapeutic action of drugs such as these coincides willi the restoration of normal plasma levels of the neurohormone in depressives. In view of the biogenic amine deficiency hypothesis of depression and the contentious claim that TDO is the major peripheral determinant of brain TRP, brain 5-HT and ultimately aMT, the regulation of TDO is investigated and discussed. The study concludes that TDO activity is regulated by a number of endogenous compounds which are mainly derivatives of TRP, such as aMT and oxidized nicotinamide adenine dinucleotide and exogenous substances, of which DMI and fluoxetine are but two. In addition, modulation of IDO activity in depression appears to be an important aspect of antidepressant action. aMT, the product of the pineal gland, also has the potential to increase plasma TRP and hence forebrain TRP levels, and ultimately 5-HT concentrations, firstly by displacing TRP from serum albumin and secondly by inhibiting TDO.
- Full Text:
Environmental education : a strategy for primary teacher education
- Authors: Wagiet, Razeena
- Date: 1997
- Subjects: Environmental education -- Study and teaching (Elementary) Environmental education -- Study and teaching (Elementary) -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1512 , http://hdl.handle.net/10962/d1003394
- Description: This research focuses on environmental education in initial teacher education, and is grounded in three interlinked and widely recognised assumptions. First, that education for sustainable living can assist in resolving some environmental problems that are contributing to the environmental crises of sustainability currently facing South Africa and the rest of the world. Second, that education for sustainable living can assist in the establishment of a new environmental ethic that will foster a sustainable way of living. Third, that teacher education is a vital process for the attainment of both. These assumptions inform the aim of this research, which is to explore the potential for the implementation of education for sustainable living, and to identify a strategy for this, for initial teacher education, for senior primary school student teachers in the Western Cape. The strategy is derived following the grounded theory approach, developed through the case study method. In the process of identifying the strategy, this study establishes that there are challenges at macro, meso and micro levels that are obstructing the changes necessary for education for sustainable living. Change theory provides the basis for explaining these shortcomings, by helping to identify the barriers that might obstruct the realisation of the changes that are necessary for education for sustainable living. These challenges need to be perceived in the light of overcoming three sets of barriers in the way of the potential implementation of education for sustainable living in teacher education. First are those that can be ascribed to the formal education system that, while clinging to Western, Eurocentric values on the one hand, bave also failed to secure a policy for environmental education on the other. Second are the barriers ascribed to the teacher educators themselves, with the whole notion of their powerlessness at its core. Finally, there are the logistical barriers, which encompass, for example, time and financial constraints. With these barriers as a backdrop, to facilitate the incorporation of environmental education into initial teacher education, the study identifies a need for the development of a strategy to secure that education for sustainable living assumes its rightful place in the curriculum for initial teacher education. This framework emerges from the theory grounded in the interviewees' responses during the research, and from the theory grounded in the literature. Central to this framework is for education for sustainable living to contribute to the realisation of real change, change that would further the transformation of our conflict-riddled and inequitable society towards a more democratic and just one. This thesis demonstrates that the realisation of the changes necessary for education for sustainable living demand a reconstruction of current teacher education in order to secure and to sustain an appropriate and sound education ethic to form the basis of a trans formative teacher education curriculum for sustainable living within initial teacher education. Except formal policy, but central to overcoming these barriers, is the need for professional development programmes for teacher educators. A strategy in this regard, is outlined.
- Full Text:
- Authors: Wagiet, Razeena
- Date: 1997
- Subjects: Environmental education -- Study and teaching (Elementary) Environmental education -- Study and teaching (Elementary) -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1512 , http://hdl.handle.net/10962/d1003394
- Description: This research focuses on environmental education in initial teacher education, and is grounded in three interlinked and widely recognised assumptions. First, that education for sustainable living can assist in resolving some environmental problems that are contributing to the environmental crises of sustainability currently facing South Africa and the rest of the world. Second, that education for sustainable living can assist in the establishment of a new environmental ethic that will foster a sustainable way of living. Third, that teacher education is a vital process for the attainment of both. These assumptions inform the aim of this research, which is to explore the potential for the implementation of education for sustainable living, and to identify a strategy for this, for initial teacher education, for senior primary school student teachers in the Western Cape. The strategy is derived following the grounded theory approach, developed through the case study method. In the process of identifying the strategy, this study establishes that there are challenges at macro, meso and micro levels that are obstructing the changes necessary for education for sustainable living. Change theory provides the basis for explaining these shortcomings, by helping to identify the barriers that might obstruct the realisation of the changes that are necessary for education for sustainable living. These challenges need to be perceived in the light of overcoming three sets of barriers in the way of the potential implementation of education for sustainable living in teacher education. First are those that can be ascribed to the formal education system that, while clinging to Western, Eurocentric values on the one hand, bave also failed to secure a policy for environmental education on the other. Second are the barriers ascribed to the teacher educators themselves, with the whole notion of their powerlessness at its core. Finally, there are the logistical barriers, which encompass, for example, time and financial constraints. With these barriers as a backdrop, to facilitate the incorporation of environmental education into initial teacher education, the study identifies a need for the development of a strategy to secure that education for sustainable living assumes its rightful place in the curriculum for initial teacher education. This framework emerges from the theory grounded in the interviewees' responses during the research, and from the theory grounded in the literature. Central to this framework is for education for sustainable living to contribute to the realisation of real change, change that would further the transformation of our conflict-riddled and inequitable society towards a more democratic and just one. This thesis demonstrates that the realisation of the changes necessary for education for sustainable living demand a reconstruction of current teacher education in order to secure and to sustain an appropriate and sound education ethic to form the basis of a trans formative teacher education curriculum for sustainable living within initial teacher education. Except formal policy, but central to overcoming these barriers, is the need for professional development programmes for teacher educators. A strategy in this regard, is outlined.
- Full Text:
A phenomenological investigation into educational leaders' perceptions of themselves, their followers, and their organisational contexts
- Authors: Van der Mescht, Hennie
- Date: 1997
- Subjects: Educational leadership -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1697 , http://hdl.handle.net/10962/d1003580
- Description: Leadership is a richly researched ana widely covered topic, attracting the attention of virtually every discipline within the human sciences. Research which focuses on leadership within an educational context is, however, a relatively recent development. Most of the leadership research conducted in this century has been driven by the needs of industry. Consequently, leadership theories have tended to emphasise the instrumentality, measurability and effectiveness of leadership. Leadership models, intent on being prescriptive and 'learnable', have been inclined to oversimplify the complexity of the leadership phenomenon. Another factor which accounts for these features is the research approach adopted by researchers, which has largely been positivistic. This study is an attempt to investigate the underlying dynamics of educational leadership. It is an in-depth study of five educational leaders' perceptions of themselves as leaders, of the people with whom they interact, and of the organisations within which they work. The study is conducted along the lines suggested by phenomenology, a highly qualitative, anti-positivistic research approach, which encourages the researcher to set aside pre-conceived notions of the phenomenon, and to describe what is found exactly as it presents itself. My findings have highlighted a wide and richly varied range of psychological, emotional and cultural factors which seem to play significant roles in how leaders perceive themselves, others and their environments. Most of these factors have been either entirely ignored or very scantily covered in mainstream leadership literature. Educational leadership emerges as a complex, virtually undefinable phenomenon; new and challenging research approaches and methods have the potential for increasing our understanding of how leaders lead, which in turn will have implications for leadership education and selection.
- Full Text:
- Authors: Van der Mescht, Hennie
- Date: 1997
- Subjects: Educational leadership -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1697 , http://hdl.handle.net/10962/d1003580
- Description: Leadership is a richly researched ana widely covered topic, attracting the attention of virtually every discipline within the human sciences. Research which focuses on leadership within an educational context is, however, a relatively recent development. Most of the leadership research conducted in this century has been driven by the needs of industry. Consequently, leadership theories have tended to emphasise the instrumentality, measurability and effectiveness of leadership. Leadership models, intent on being prescriptive and 'learnable', have been inclined to oversimplify the complexity of the leadership phenomenon. Another factor which accounts for these features is the research approach adopted by researchers, which has largely been positivistic. This study is an attempt to investigate the underlying dynamics of educational leadership. It is an in-depth study of five educational leaders' perceptions of themselves as leaders, of the people with whom they interact, and of the organisations within which they work. The study is conducted along the lines suggested by phenomenology, a highly qualitative, anti-positivistic research approach, which encourages the researcher to set aside pre-conceived notions of the phenomenon, and to describe what is found exactly as it presents itself. My findings have highlighted a wide and richly varied range of psychological, emotional and cultural factors which seem to play significant roles in how leaders perceive themselves, others and their environments. Most of these factors have been either entirely ignored or very scantily covered in mainstream leadership literature. Educational leadership emerges as a complex, virtually undefinable phenomenon; new and challenging research approaches and methods have the potential for increasing our understanding of how leaders lead, which in turn will have implications for leadership education and selection.
- Full Text:
Share-net: a case study of environmental education resource material development in a risk society
- Authors: Taylor, Robert James
- Date: 1997
- Subjects: Environmental education Environmental education -- South Africa Environmental education -- Activity programs -- South Africa Environmental education -- South Africa -- Information resources
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1765 , http://hdl.handle.net/10962/d1003650
- Description: This narrative study reviews the development of Share-Net, an informal resource materials network, located within the developing environmental education activities of the Wildlife and Environment Society of South Africa. Historical shaping factors within the Society are discussed and changing views on conservation, environmental education and research are described. Rather than the research process being a utilitarian and outside endeavour for clarifying and monitoring, the research orientation of this study is one of grounded activity within and integrally part of processes of change. Within a developing story of resource materials and workshops, themes are traced and emergent tensions are critically reviewed. Teacher workshops from 1981 until 1995 are analysed and developing orientations are described. Within this review changes are evident from an orientation of 'us' informing 'them' to joint, collaborative endeavours within the development and use of resource materials. The study reviews and illuminates the Share-Net project around questions of project orientation and management and this is done through the narration of case studies. Principles and patterns emerging within these are examined to guide future resource development projects. Popularist notions of networking and structural functionalist notions of social change are also examined. Grand, modernist strategies designed to cause change in others are questioned and the role of small-scale nodes of resource material activity cooperating within an open network are clarified as useful alternatives. Local resourcing centres such as these have proved useful in supporting teachers in the development, use and adaptation of resource materials. The study also examines income and expenditure of the Share-Net project since 1988 to review economic sustainability. A case is made for sustaining the project conceptually and financially through the sales of products and services rather than through external donor sources. Finally the study raises questions and challenges within the project and proposes guiding frameworks for future review, in action, as the story continues.
- Full Text:
- Authors: Taylor, Robert James
- Date: 1997
- Subjects: Environmental education Environmental education -- South Africa Environmental education -- Activity programs -- South Africa Environmental education -- South Africa -- Information resources
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1765 , http://hdl.handle.net/10962/d1003650
- Description: This narrative study reviews the development of Share-Net, an informal resource materials network, located within the developing environmental education activities of the Wildlife and Environment Society of South Africa. Historical shaping factors within the Society are discussed and changing views on conservation, environmental education and research are described. Rather than the research process being a utilitarian and outside endeavour for clarifying and monitoring, the research orientation of this study is one of grounded activity within and integrally part of processes of change. Within a developing story of resource materials and workshops, themes are traced and emergent tensions are critically reviewed. Teacher workshops from 1981 until 1995 are analysed and developing orientations are described. Within this review changes are evident from an orientation of 'us' informing 'them' to joint, collaborative endeavours within the development and use of resource materials. The study reviews and illuminates the Share-Net project around questions of project orientation and management and this is done through the narration of case studies. Principles and patterns emerging within these are examined to guide future resource development projects. Popularist notions of networking and structural functionalist notions of social change are also examined. Grand, modernist strategies designed to cause change in others are questioned and the role of small-scale nodes of resource material activity cooperating within an open network are clarified as useful alternatives. Local resourcing centres such as these have proved useful in supporting teachers in the development, use and adaptation of resource materials. The study also examines income and expenditure of the Share-Net project since 1988 to review economic sustainability. A case is made for sustaining the project conceptually and financially through the sales of products and services rather than through external donor sources. Finally the study raises questions and challenges within the project and proposes guiding frameworks for future review, in action, as the story continues.
- Full Text:
Bioaccumulation of heavy metals by the yeast S. cerevisiae and the bioremediation of industrial waste water
- Authors: Stoll, Anita
- Date: 1997
- Subjects: Saccharomyces cerevisiae Yeast fungi -- Biotechnology Metal ions Bioremediation Water -- Purification -- Biological treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4015 , http://hdl.handle.net/10962/d1004075
- Description: Water is an essential element in all aspects of life and is vital for both domestic and industrial purposes regarding both the quality and quantity thereof. Similar to many other drought stricken countries, South Africa requires water for the socio-economic growth of the country, yet is faced with the problem of maintaining the quality of its drinking water as well as protecting the dwindling supplies. In an attempt to prevent the deterioration of South African water supplies the treatment, purification and recycling of industrial and mining waste water has recently become of prime importance. Many industrial and mining waste waters contain heavy metals in toxic quantities. The conventional processes that have been used till recently to address this problem, are often expensive or contain chemical agents which compound the environmental problem. As an alternative biological methods of metal accumulation appear to offer an economic and efficient alternative to these methods. An advantage to the South African scenario is the commercial production of the yeast, S. cerevisiae as a readily inexpensive by-product from some fermentation industries, Yeast cells, and in particular S. cerevisiae have proven to be capable of accumulating heavy metals, and therefore exhibit potential application in the bioremediation of waste water. The aim of this project was twofold. The initial part of this work attempted to define the mechanisms of metal accumulation by the yeast cells and cellular components. The information obtained from these initial studies provided a data base required for the development of a bioremediation system. Initial contact with the metal ions occurs at the wall interface of the yeast cell. Metal accumulation appears to be a function of all the cell wall components. The isolated cell wall components are better metal chelators then the intact cell walls. An apparent affinity series of mannan > chitin> glucan > intact cell walls exists. However, these components differ in their affinities for metal ions. Storage of metal ions within the cell occurs predominantly in the vacuole. The present study concluded that metal accumulation by the vacuole could be related to size. Metal accumulation occurred in the order of Cu2+ > Co2+ > Cd2+ with a corresponding decrease in atomic radii of Cd2+ > C02+ > Cu2+. Vacuolar ion deposition occurs at an early stage during the internalization of metal ions within the yeast cells. At the onset of vacuolar saturation, depositions of metal ions as granules within the cytosol occurs. In the presence of heavy metal cations viable yeast cells can be shown to exhibit two types of cellular responses. Uptake of Cu2+ and Cd2+ causes the loss of intracellular physiological cations from within the yeast cell. In comparison, uptake of Co2+ into the cell does not have this effect. All three heavy metal cations initiate plasma cell membrane permeability, thus the Cu2+ and Cd2+ induced loss of the intracellular cations, occurs. ~ a result of ion-exchange mechanisms and not due to cation leakage brought about by membrane permeabilization. Uptake of heavy metals by viable yeasts appears to be generally non-selective though the amount of metals accumulated are largely affected by the ratio of ambient metal concentration to biomass quantity. In addition, the energy dependent nature of internalization necessitates the availability of an external energy source for metal uptake by viable yeast cells. For these reasons metal removal from industrial waste water was investigated using non-viable biomass. By immobilizing the yeast cells additional mechanical integrity and stability was conferred apon the biomass. The three types of biomass preparations developed in this study, viz. polyvinyl alcohol (PV A) Na-alginate, PV A Na-orthophosphate and alkali treated polyethylenimine (PEI):glutaraldehyde (GA) biomass pellets, all fulfilled the necessary physical requirements. However, the superior metal accumulating properties of the PEI:GA biomass determined its selection as a biosorbent for bioremediation purposes. Biosorption of heavy metals by PEI:GA biomass is of a competitive nature, with the amount of metal accumulated influenced by the availability of the metal ions. This availability is largely determined by the solution pH. At low pH values the affinity of the biomass for metals decreases, whilst enhanced metal biosorption occurs at higher pHs, ego pH 4.5 - 6.0. PEI:GA biomass pellets can be implemented -as a biosorbent for the bi9remediaiton of high concentration, low-volume metal containing industrial waste. Several options regarding the bioremediation system are available. Depending on the concentration of the metals in the effluent, the bioremediation process can either be used independently or as part of a biphasic remediation system for the treatment of waste water. Initial phase chemical modification may be required, whilst two types of biological systems can be implemented as 'part of the second phase. The PEI:GA biomass can either be contained within continuous-flow fixed bed tanks or continuous-flow stirred bioreactor tanks. Due to the simplicity of the process and the ease with which scale-up is facilitated, the second type of system shows greater application potential for the treatment of this type of industrial waste water than the fixed-bed systems.
- Full Text:
- Authors: Stoll, Anita
- Date: 1997
- Subjects: Saccharomyces cerevisiae Yeast fungi -- Biotechnology Metal ions Bioremediation Water -- Purification -- Biological treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4015 , http://hdl.handle.net/10962/d1004075
- Description: Water is an essential element in all aspects of life and is vital for both domestic and industrial purposes regarding both the quality and quantity thereof. Similar to many other drought stricken countries, South Africa requires water for the socio-economic growth of the country, yet is faced with the problem of maintaining the quality of its drinking water as well as protecting the dwindling supplies. In an attempt to prevent the deterioration of South African water supplies the treatment, purification and recycling of industrial and mining waste water has recently become of prime importance. Many industrial and mining waste waters contain heavy metals in toxic quantities. The conventional processes that have been used till recently to address this problem, are often expensive or contain chemical agents which compound the environmental problem. As an alternative biological methods of metal accumulation appear to offer an economic and efficient alternative to these methods. An advantage to the South African scenario is the commercial production of the yeast, S. cerevisiae as a readily inexpensive by-product from some fermentation industries, Yeast cells, and in particular S. cerevisiae have proven to be capable of accumulating heavy metals, and therefore exhibit potential application in the bioremediation of waste water. The aim of this project was twofold. The initial part of this work attempted to define the mechanisms of metal accumulation by the yeast cells and cellular components. The information obtained from these initial studies provided a data base required for the development of a bioremediation system. Initial contact with the metal ions occurs at the wall interface of the yeast cell. Metal accumulation appears to be a function of all the cell wall components. The isolated cell wall components are better metal chelators then the intact cell walls. An apparent affinity series of mannan > chitin> glucan > intact cell walls exists. However, these components differ in their affinities for metal ions. Storage of metal ions within the cell occurs predominantly in the vacuole. The present study concluded that metal accumulation by the vacuole could be related to size. Metal accumulation occurred in the order of Cu2+ > Co2+ > Cd2+ with a corresponding decrease in atomic radii of Cd2+ > C02+ > Cu2+. Vacuolar ion deposition occurs at an early stage during the internalization of metal ions within the yeast cells. At the onset of vacuolar saturation, depositions of metal ions as granules within the cytosol occurs. In the presence of heavy metal cations viable yeast cells can be shown to exhibit two types of cellular responses. Uptake of Cu2+ and Cd2+ causes the loss of intracellular physiological cations from within the yeast cell. In comparison, uptake of Co2+ into the cell does not have this effect. All three heavy metal cations initiate plasma cell membrane permeability, thus the Cu2+ and Cd2+ induced loss of the intracellular cations, occurs. ~ a result of ion-exchange mechanisms and not due to cation leakage brought about by membrane permeabilization. Uptake of heavy metals by viable yeasts appears to be generally non-selective though the amount of metals accumulated are largely affected by the ratio of ambient metal concentration to biomass quantity. In addition, the energy dependent nature of internalization necessitates the availability of an external energy source for metal uptake by viable yeast cells. For these reasons metal removal from industrial waste water was investigated using non-viable biomass. By immobilizing the yeast cells additional mechanical integrity and stability was conferred apon the biomass. The three types of biomass preparations developed in this study, viz. polyvinyl alcohol (PV A) Na-alginate, PV A Na-orthophosphate and alkali treated polyethylenimine (PEI):glutaraldehyde (GA) biomass pellets, all fulfilled the necessary physical requirements. However, the superior metal accumulating properties of the PEI:GA biomass determined its selection as a biosorbent for bioremediation purposes. Biosorption of heavy metals by PEI:GA biomass is of a competitive nature, with the amount of metal accumulated influenced by the availability of the metal ions. This availability is largely determined by the solution pH. At low pH values the affinity of the biomass for metals decreases, whilst enhanced metal biosorption occurs at higher pHs, ego pH 4.5 - 6.0. PEI:GA biomass pellets can be implemented -as a biosorbent for the bi9remediaiton of high concentration, low-volume metal containing industrial waste. Several options regarding the bioremediation system are available. Depending on the concentration of the metals in the effluent, the bioremediation process can either be used independently or as part of a biphasic remediation system for the treatment of waste water. Initial phase chemical modification may be required, whilst two types of biological systems can be implemented as 'part of the second phase. The PEI:GA biomass can either be contained within continuous-flow fixed bed tanks or continuous-flow stirred bioreactor tanks. Due to the simplicity of the process and the ease with which scale-up is facilitated, the second type of system shows greater application potential for the treatment of this type of industrial waste water than the fixed-bed systems.
- Full Text:
Termination of psychotherapy : a phenomenological exploration of the therapist's and the patient's experience
- Authors: Schlodder, Michelle Ingrid
- Date: 1997
- Subjects: Psychotherapy -- termination Psychotherapy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3049 , http://hdl.handle.net/10962/d1002558
- Full Text:
- Authors: Schlodder, Michelle Ingrid
- Date: 1997
- Subjects: Psychotherapy -- termination Psychotherapy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3049 , http://hdl.handle.net/10962/d1002558
- Full Text:
Synthetic and physical organic studies of chromone derivatives
- Ramaite, Ipfani David Isaiah
- Authors: Ramaite, Ipfani David Isaiah
- Date: 1997
- Subjects: Benzopyrans Heterocyclic compounds -- Derivatives Coumarins
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4380 , http://hdl.handle.net/10962/d1005045
- Description: A range of chromone-2-carboxylic acids has been prepared by condensing suitably substituted 2-hydroxyacetophenones with diethyl oxalate. pK₂ Studies of these acids revealed that 6- or 7-methoxy substituents decreased acidity while the 6-nitro group enhanced acidity; the strongest acid was the 3-chloro derivative, the increase in acidity being attributed to steric inhibition of acid-weakening delocalisation between the carboxyl group and the chromone system. Various chromone-2-carboxamides, derived from acid chloride precursors, were converted to polysubstituted acrylamides by nucleophilic ring-opening with selected amine nucleophiles. The main fragmentation patterns exhibited by these acrylamides were elucidated using a combination of low resolution, high resolution and meta-stable peak analysis, while the effect of substituents on the simultaneous internal rotation involving the carboxamide and enamine moieties were studied using dynamic NMR spectroscopy. Rotational barriers of ca. 67.1 kJmol ̄¹ and ca. 102 kJmol ̄¹ were found for the enamine and amide rotors, respectively. Several synthetic pathways were followed to prepare a series of 2-(N,N-dialkylamino)chromones which were subjected to detailed mass spectral analysis. In addition to substituent-specific fragmentations , the 2-aminochromones appear to fragment via 3 major pathways. The effect of substituents on the internal rotation of the amino moeity was investigated by variable temperature ¹H NMR spectroscopy and the resulting DNMR data was used to calculate the rotational barriers. Examination of the data reveals that the electron-releasing 6- and 7- substituents reduce the C-NMe₂ rotational barrier to ca. 43.5 kJmol ̄¹ , while the nitro analogue has the largest rotational barrier (ca. 46.1 kJmol ̄¹) because of the electron-withdrawing effect of this substituent.
- Full Text:
- Authors: Ramaite, Ipfani David Isaiah
- Date: 1997
- Subjects: Benzopyrans Heterocyclic compounds -- Derivatives Coumarins
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4380 , http://hdl.handle.net/10962/d1005045
- Description: A range of chromone-2-carboxylic acids has been prepared by condensing suitably substituted 2-hydroxyacetophenones with diethyl oxalate. pK₂ Studies of these acids revealed that 6- or 7-methoxy substituents decreased acidity while the 6-nitro group enhanced acidity; the strongest acid was the 3-chloro derivative, the increase in acidity being attributed to steric inhibition of acid-weakening delocalisation between the carboxyl group and the chromone system. Various chromone-2-carboxamides, derived from acid chloride precursors, were converted to polysubstituted acrylamides by nucleophilic ring-opening with selected amine nucleophiles. The main fragmentation patterns exhibited by these acrylamides were elucidated using a combination of low resolution, high resolution and meta-stable peak analysis, while the effect of substituents on the simultaneous internal rotation involving the carboxamide and enamine moieties were studied using dynamic NMR spectroscopy. Rotational barriers of ca. 67.1 kJmol ̄¹ and ca. 102 kJmol ̄¹ were found for the enamine and amide rotors, respectively. Several synthetic pathways were followed to prepare a series of 2-(N,N-dialkylamino)chromones which were subjected to detailed mass spectral analysis. In addition to substituent-specific fragmentations , the 2-aminochromones appear to fragment via 3 major pathways. The effect of substituents on the internal rotation of the amino moeity was investigated by variable temperature ¹H NMR spectroscopy and the resulting DNMR data was used to calculate the rotational barriers. Examination of the data reveals that the electron-releasing 6- and 7- substituents reduce the C-NMe₂ rotational barrier to ca. 43.5 kJmol ̄¹ , while the nitro analogue has the largest rotational barrier (ca. 46.1 kJmol ̄¹) because of the electron-withdrawing effect of this substituent.
- Full Text:
Vitamin E supplementation and secondary metabolites interactions and effects on melanoma growth
- Authors: Ottino, Paulo
- Date: 1997
- Subjects: Vitamin E Melanoma
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4016 , http://hdl.handle.net/10962/d1004076
- Description: The present study was undertaken to determine the effects and possible mechanism of action of vitamin E succinate on malignant murine melanoma (BL6) and non-malignant monkey kidney (LLCMK) cell growth in vitro. Studies revealed that supplementation of 5, 7 and lOJLg/ml vitamin E succinate significantly inhibited BL6 cell growth, while in LLCMK cells no significant increase or decrease in growth was observed. The actual mechanism by which vitamin E succinate inhibits BL6 cell growth is at present unclear. Studies have suggested a radical or oxidant involvement in a number of degenerative diseases such as cancer, and that supplementation of antioxidant vitamins such as vitamin E may function to reduce cancer cell growth by quenching free radical species and preventing lipid peroxidation. In addition to its antioxidant role in a cell, vitamin E is believed to modulate the activities of various enzymes and metabolites in the eicosanoid pathway. Hence, this study investigated the effects of vitamin E succinate supplementation on free radical and lipid peroxidation levels, as well as the activities of various enzymes and metabolites ill the eicosanoid pathway. Throughout this study, emphasis was placed on BL6 melanoma cells since the magnitude of the relationship between LLCMK growth and the levels of various enzymes and metabolites in the eicosanoid pathway varied considerably from one experiment to another and did not show the consistent trend found with the BL6 cells. A decrease in cell growth was found to be accompanied by a concomitant increase rather than a decrease in the levels of free radicals and lipid peroxidation, suggesting that the growth inhibitory effects of vitamin E succinate on BL6 cells in vitro was not due to its antioxidant properties associated with the vitamin E component, but rather due to one or more of its other potential roles within the cell. This proposal was further strengthened by findings that vitamin E succinate, a non-physiological antioxidant in its esterified form, did not undergo significant cleavage to free vitamin E in the BL6 cells. Vitamin E succinate is believed to modulate membrane bound enzyme activities through physicochemical interactions with membrane lipids and changes in membrane fluidity. Hence, this study investigated the role of vitamin E succinate in modulating the activity of various enzymes and secondary messengers in the eicosanoid pathway. Supplementation of l-lOjLg/ml vitamin E succinate resulted in an overall increase in phospholipase A2 activity while cyclooxygenase and adenyl ate cyclase activities were found to be significantly increased at vitamin E succinate concentrations of 7 and WjLg/ml respectively. A significant increase in" 5-LOX activity was observed a! 10jLg/mi supplementation. The suggestion that vitamin E succinate modulates membrane bound enzyme activities was further strengthened by uptake and cellular distribution studies, which showed significantly higher levels of vitamin E succinate in membrane fractions of BL6 cells when compared with stroma fractions. Another factor which could account for elevated PLA2,-5-LOX and COX activities in BL6 cells as a result of vitamin E succinate supplementation, was that of intracellular calcium levels. Supplementation of BL6 cells with 1-7 jLg/ml vitamin E succinate resulted in an overall increase in intracellular calcium levels. These changes in calcium levels however were positively correlated with changes in PLA2 activity only. Since the rate of prostaglandin synthesis is controlled by phospholipase A2 activity, and net prostagiandin production is dependant on cyclooxygenase activity, the effects of vitamin E succinate supplementation on prostaglandin levels in BL6 cells was determined. Vitamin E succinate supplementation resulted in a significant decrease in prostaglandin D2 levels at vitamin E succinate concentrations of 3, 5, 7 and lOjLg/ml respectively, while prostaglandin F2a levels were significantly decreased at 1-10jLg/ml vitamin E succinate. The increases in prostaglandin E2 and 12 levels were inversely related to BL6 cell growth suggesting that both prostaglandins may act as negative regulators of BL6 cell growth. When comparing prostaglandin E2 levels to prostaglandin 12 levels in BL6 cells, significantly higher levels of prostaglandin E2 were found, suggesting that vitamin E succinate effects were mediated primarily through an increase in prostaglandin E2 levels. Furthermore, prostaglandin E2 levels are believed to modulate adenylate cyclase activity. It is therefore reasonable to conclude that the increased adenyl ate cyclase activity found in BL6 cells was dependant on prostaglandin E2 levels, since increases in prostaglandin E2 levels at 7 and lOjLg/ml vitamin E succinate correlated with an increase in adenylate cyclase activity and cyclic adenosine monophosphate levels. Thus it appeared that the observed inhibitory effects of vitamin E succinate supplementation on BL6 cell growth was not due to the antioxidant properties associated with the vitamin E component of the vitamin E succinate molecule, but was rather mediated in part through a cascade effect initiated by phospholipase A2 activation and archidonic acid release. This initial effect then appeared to result in an increase in cyclooxygenase activity and activation of a prostaglandin E2-adenylate cyclase-cyclic adenosine monophosphate linked system, ultimately altering cyclic adenosine monophosphate levels and inhibiting BL6 cell growth. This was confirmed when BL6 cells were supplemented with indomethacin, a cyclooxygenase inhibitor. Supplementation with the inhibitor resulted in vitamin E succinate having no inhibitory effects on BL6 cell growth. Furthermore, when comparing the levels of prostaglandin ~, adenylate cyclase activity and cyclIC adenosine monophosphate in the indomethacin treated cultures to non-indomethacin treated cultures, markedly lower levels of these metabolites were found in the indomethacin treated cultures. The cause of the increase in free radical and lipid peroxidation levels in BL6 cells following vitamin E succinate supplementation was further investigated. Cyclooxygenase enzymes are believed to generate free radical species and contribute to lipid peroxidation levels during catalytic activity. Markedly lower levels of free radicals and lipid peroxidation in indomethacin treated cultures were found when compared with vitamin E succinate treated cultures alone, suggesting that the increases in free radical and lipid peroxidation levels in BL6 cells supplemented with vitamin E succinate were indirectly due to an increase in cyclooxygenase activity in these cells.
- Full Text:
- Authors: Ottino, Paulo
- Date: 1997
- Subjects: Vitamin E Melanoma
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4016 , http://hdl.handle.net/10962/d1004076
- Description: The present study was undertaken to determine the effects and possible mechanism of action of vitamin E succinate on malignant murine melanoma (BL6) and non-malignant monkey kidney (LLCMK) cell growth in vitro. Studies revealed that supplementation of 5, 7 and lOJLg/ml vitamin E succinate significantly inhibited BL6 cell growth, while in LLCMK cells no significant increase or decrease in growth was observed. The actual mechanism by which vitamin E succinate inhibits BL6 cell growth is at present unclear. Studies have suggested a radical or oxidant involvement in a number of degenerative diseases such as cancer, and that supplementation of antioxidant vitamins such as vitamin E may function to reduce cancer cell growth by quenching free radical species and preventing lipid peroxidation. In addition to its antioxidant role in a cell, vitamin E is believed to modulate the activities of various enzymes and metabolites in the eicosanoid pathway. Hence, this study investigated the effects of vitamin E succinate supplementation on free radical and lipid peroxidation levels, as well as the activities of various enzymes and metabolites ill the eicosanoid pathway. Throughout this study, emphasis was placed on BL6 melanoma cells since the magnitude of the relationship between LLCMK growth and the levels of various enzymes and metabolites in the eicosanoid pathway varied considerably from one experiment to another and did not show the consistent trend found with the BL6 cells. A decrease in cell growth was found to be accompanied by a concomitant increase rather than a decrease in the levels of free radicals and lipid peroxidation, suggesting that the growth inhibitory effects of vitamin E succinate on BL6 cells in vitro was not due to its antioxidant properties associated with the vitamin E component, but rather due to one or more of its other potential roles within the cell. This proposal was further strengthened by findings that vitamin E succinate, a non-physiological antioxidant in its esterified form, did not undergo significant cleavage to free vitamin E in the BL6 cells. Vitamin E succinate is believed to modulate membrane bound enzyme activities through physicochemical interactions with membrane lipids and changes in membrane fluidity. Hence, this study investigated the role of vitamin E succinate in modulating the activity of various enzymes and secondary messengers in the eicosanoid pathway. Supplementation of l-lOjLg/ml vitamin E succinate resulted in an overall increase in phospholipase A2 activity while cyclooxygenase and adenyl ate cyclase activities were found to be significantly increased at vitamin E succinate concentrations of 7 and WjLg/ml respectively. A significant increase in" 5-LOX activity was observed a! 10jLg/mi supplementation. The suggestion that vitamin E succinate modulates membrane bound enzyme activities was further strengthened by uptake and cellular distribution studies, which showed significantly higher levels of vitamin E succinate in membrane fractions of BL6 cells when compared with stroma fractions. Another factor which could account for elevated PLA2,-5-LOX and COX activities in BL6 cells as a result of vitamin E succinate supplementation, was that of intracellular calcium levels. Supplementation of BL6 cells with 1-7 jLg/ml vitamin E succinate resulted in an overall increase in intracellular calcium levels. These changes in calcium levels however were positively correlated with changes in PLA2 activity only. Since the rate of prostaglandin synthesis is controlled by phospholipase A2 activity, and net prostagiandin production is dependant on cyclooxygenase activity, the effects of vitamin E succinate supplementation on prostaglandin levels in BL6 cells was determined. Vitamin E succinate supplementation resulted in a significant decrease in prostaglandin D2 levels at vitamin E succinate concentrations of 3, 5, 7 and lOjLg/ml respectively, while prostaglandin F2a levels were significantly decreased at 1-10jLg/ml vitamin E succinate. The increases in prostaglandin E2 and 12 levels were inversely related to BL6 cell growth suggesting that both prostaglandins may act as negative regulators of BL6 cell growth. When comparing prostaglandin E2 levels to prostaglandin 12 levels in BL6 cells, significantly higher levels of prostaglandin E2 were found, suggesting that vitamin E succinate effects were mediated primarily through an increase in prostaglandin E2 levels. Furthermore, prostaglandin E2 levels are believed to modulate adenylate cyclase activity. It is therefore reasonable to conclude that the increased adenyl ate cyclase activity found in BL6 cells was dependant on prostaglandin E2 levels, since increases in prostaglandin E2 levels at 7 and lOjLg/ml vitamin E succinate correlated with an increase in adenylate cyclase activity and cyclic adenosine monophosphate levels. Thus it appeared that the observed inhibitory effects of vitamin E succinate supplementation on BL6 cell growth was not due to the antioxidant properties associated with the vitamin E component of the vitamin E succinate molecule, but was rather mediated in part through a cascade effect initiated by phospholipase A2 activation and archidonic acid release. This initial effect then appeared to result in an increase in cyclooxygenase activity and activation of a prostaglandin E2-adenylate cyclase-cyclic adenosine monophosphate linked system, ultimately altering cyclic adenosine monophosphate levels and inhibiting BL6 cell growth. This was confirmed when BL6 cells were supplemented with indomethacin, a cyclooxygenase inhibitor. Supplementation with the inhibitor resulted in vitamin E succinate having no inhibitory effects on BL6 cell growth. Furthermore, when comparing the levels of prostaglandin ~, adenylate cyclase activity and cyclIC adenosine monophosphate in the indomethacin treated cultures to non-indomethacin treated cultures, markedly lower levels of these metabolites were found in the indomethacin treated cultures. The cause of the increase in free radical and lipid peroxidation levels in BL6 cells following vitamin E succinate supplementation was further investigated. Cyclooxygenase enzymes are believed to generate free radical species and contribute to lipid peroxidation levels during catalytic activity. Markedly lower levels of free radicals and lipid peroxidation in indomethacin treated cultures were found when compared with vitamin E succinate treated cultures alone, suggesting that the increases in free radical and lipid peroxidation levels in BL6 cells supplemented with vitamin E succinate were indirectly due to an increase in cyclooxygenase activity in these cells.
- Full Text:
Breaking the karmic complex: the role of transpersonal phenomena in psychotherapy with an adult survivor of child abuse : a clinical case study
- Authors: Oberholzer, Sofia Adriana
- Date: 1997
- Subjects: Transpersonal psychology Reincarnation Adult child abuse victims -- Treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3034 , http://hdl.handle.net/10962/d1002543
- Description: This longitudinal study addresses the interface between the emerging paradigm of Transpersonal Psychology, which recognizes the transbiographical domain of consciousness, and the clinical area of child abuse. By using the transpersonal concept of the karmic complex, which was developed in the discourse of Roger Woolger, it explores the clinical value of past-life regressions and other transpersonal phenomena in the therapeutic process. The study provides an in-depth phenomenological description and a theoreticalheuristic explication of the experience of a single subject who was an adult survivor of physical and sexual abuse as well as early emotional deprivation in childhood, and who, over a four-year period of intensive psychotherapy, experienced a wide spectrum of transpersonal phenomena which included 123 past-life regressions. The therapeutic process could be clearly delineated into a biographical phase, which was dominated by biographical traumas, and a subsequ~nt transpersonal phase, during which the focus was almost exclusively on transpersonal material. This provided the opportunity to explore the therapeutic impact of trans personal mechanisms" of healing on an interlocking constellation of complexes in terms of achieving an integration of archetypal opposites in the psyche. The study provides strong support for transpersonal concepts about the nature of consciousness, as developed by Grof and Woolger. It affirms spirituality as an intrinsic property of the psyche, and verifies that the healing of psychological trauma is on a continuum with the process of spiritual purification and growth. The ,study establishes support for the clinical value of the karmic complex as a conceptual tool and provides a systematic, extended analysis of the multi levelled processes involved in the breaking of the complex.
- Full Text:
- Authors: Oberholzer, Sofia Adriana
- Date: 1997
- Subjects: Transpersonal psychology Reincarnation Adult child abuse victims -- Treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3034 , http://hdl.handle.net/10962/d1002543
- Description: This longitudinal study addresses the interface between the emerging paradigm of Transpersonal Psychology, which recognizes the transbiographical domain of consciousness, and the clinical area of child abuse. By using the transpersonal concept of the karmic complex, which was developed in the discourse of Roger Woolger, it explores the clinical value of past-life regressions and other transpersonal phenomena in the therapeutic process. The study provides an in-depth phenomenological description and a theoreticalheuristic explication of the experience of a single subject who was an adult survivor of physical and sexual abuse as well as early emotional deprivation in childhood, and who, over a four-year period of intensive psychotherapy, experienced a wide spectrum of transpersonal phenomena which included 123 past-life regressions. The therapeutic process could be clearly delineated into a biographical phase, which was dominated by biographical traumas, and a subsequ~nt transpersonal phase, during which the focus was almost exclusively on transpersonal material. This provided the opportunity to explore the therapeutic impact of trans personal mechanisms" of healing on an interlocking constellation of complexes in terms of achieving an integration of archetypal opposites in the psyche. The study provides strong support for transpersonal concepts about the nature of consciousness, as developed by Grof and Woolger. It affirms spirituality as an intrinsic property of the psyche, and verifies that the healing of psychological trauma is on a continuum with the process of spiritual purification and growth. The ,study establishes support for the clinical value of the karmic complex as a conceptual tool and provides a systematic, extended analysis of the multi levelled processes involved in the breaking of the complex.
- Full Text:
Detached harmonies : a study in/on developing social processes of environmental education in eastern southern Africa
- Authors: O'Donoghue, Rob
- Date: 1997
- Subjects: Environmental education -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1939 , http://hdl.handle.net/10962/d1007726
- Description: Long-term social processes are explored to examine the shaping of environmental education in eastern southern Africa. The study opens with early Nguni social figurations when 'to conserve was to hunt.' It then examines colonial conservation on the frontiers of imperial expansion and developing struggles for and against wildlife preservation. These processes shaped an inversion of earlier harmonies as declining wildlife was protected in island sanctuaries of natural wilderness and 'to conserve was not to hunt.' Inside protected areas, conservation management struggles shaped new harmonies of interdependence in nature, enabling better steering choices in developing conservation science institutions. Here more reality congruent knowledge also revealed escalating risk which was linked to a lack of awareness amongst communities of 'others' outside. Within continuing conservation struggles, education in, about and for the environment emerged as new institutional processes of social control. The study examines wilderness experience, interpretation, extension, conservancies and the development of an environmental education field centre, a teacher education programme and a school curriculum. Naming and clarifying the emergent education game for reshaping the awareness and behaviour of others is examined within a developing figuration of environmental education specialists. Particular attention is given to academic and statutory processes shaping environmental education as a field of objective principles and rational processes within modernist continuities and discontinuities into the 1990's. An environmental education field centre, an earth-love curriculum and research on reserve neighbour interaction are examined as political sociologies developing within a declining power gradient and wide ranging socio-political change. Into the present, a final window on a local case of water pollution examines shifting relational dynamics revealing how environment and development education models of process may have little resonance amidst long-term socio-historical struggles and shifting controls over surroundings, others and self. A concluding review suggests that grounded critical processes engaging somewhat blind control over surroundings may yet reshape self-control and social control amongst others. The trajectories of these clarifying struggles must remain open-ended as sedimented myth and memory is reshaped within ongoing processes of escalating risk and global intermeshing.
- Full Text:
- Authors: O'Donoghue, Rob
- Date: 1997
- Subjects: Environmental education -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1939 , http://hdl.handle.net/10962/d1007726
- Description: Long-term social processes are explored to examine the shaping of environmental education in eastern southern Africa. The study opens with early Nguni social figurations when 'to conserve was to hunt.' It then examines colonial conservation on the frontiers of imperial expansion and developing struggles for and against wildlife preservation. These processes shaped an inversion of earlier harmonies as declining wildlife was protected in island sanctuaries of natural wilderness and 'to conserve was not to hunt.' Inside protected areas, conservation management struggles shaped new harmonies of interdependence in nature, enabling better steering choices in developing conservation science institutions. Here more reality congruent knowledge also revealed escalating risk which was linked to a lack of awareness amongst communities of 'others' outside. Within continuing conservation struggles, education in, about and for the environment emerged as new institutional processes of social control. The study examines wilderness experience, interpretation, extension, conservancies and the development of an environmental education field centre, a teacher education programme and a school curriculum. Naming and clarifying the emergent education game for reshaping the awareness and behaviour of others is examined within a developing figuration of environmental education specialists. Particular attention is given to academic and statutory processes shaping environmental education as a field of objective principles and rational processes within modernist continuities and discontinuities into the 1990's. An environmental education field centre, an earth-love curriculum and research on reserve neighbour interaction are examined as political sociologies developing within a declining power gradient and wide ranging socio-political change. Into the present, a final window on a local case of water pollution examines shifting relational dynamics revealing how environment and development education models of process may have little resonance amidst long-term socio-historical struggles and shifting controls over surroundings, others and self. A concluding review suggests that grounded critical processes engaging somewhat blind control over surroundings may yet reshape self-control and social control amongst others. The trajectories of these clarifying struggles must remain open-ended as sedimented myth and memory is reshaped within ongoing processes of escalating risk and global intermeshing.
- Full Text:
A comparative study of the Grey-headed Sparrow (Passer griseus L) and the House Sparrow (Passer domesticus L) in Malawi
- Authors: Nhlane, Martin Edwin Darwin
- Date: 1997
- Subjects: English sparrow Sparrows
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5654 , http://hdl.handle.net/10962/d1005337
- Description: The House Sparrow Passer domesticus, an introduced species, and the Grey-headed Sparrow Passer griseus, an indigenous species, are sympatric in Malawi. Their distribution in the country and any possible interactions were studied, principally in southern Malawi. A morphological analysis of museum specimens confirmed that grey-headed sparrows in Malawi belong to the Northern Grey-headed Sparrow Passer griseus as distinct from the Southern Grey-headed Sparrow Passer diffusus. This species was widely distributed in the, country in association with human dwellings, both in rural areas as well as urban centres. In the northern region Greyheaded Sparrows were more abundant in the urban centres than rural areas, but in the central and southern regions numbers in the rural and urban areas were more or less the same. In Blantyre City, where they are in sympatry with the House Sparrow, they were found in the low density and industrial areas and were absent from the high density areas. The House Sparrow, arrived in Malawi in 1967 at Chileka in the southern region. Since then it has spread northwards, moving from the southern to the central and northern regions. House Sparrow numbers were found to be progressively larger in the southern region and lowest in the northern region. House Sparrows were found at sites where food was readily available, as in the immediate vicinity of houses. In the central and northern regions they were restricted mainly to urban areas. In the southern region, they occur both in rural and urban areas, probably as a reflection of the larger period of colonization in the south. In the northern region their movement has apparently been restricted by geographical barriers. In Blantyre City Grey-headed Sparrows preferred areas where tree density was high and house density was low, while House Sparrows preferred areas where house density was high and tree density was low. There was a positive correlation between Greyheaded Sparrow numbers and tree density and a negative correlation with house density. House Sparrow abundance was negatively correlated with tree density and positively correlated with house density. Grey-headed Sparrows bred in the rainy season, whereas House Sparrows bred throughout the year. There were differences in nest site selection: Grey-headed Sparrows used artificial structures such as fencing poles, and wooden telephone or electricity poles. The House Sparrow used mostly buildings and nested in crevices, holes in walls and between the walls and rafters. Nest height also differed- Grey-headed Sparrows nested at heights ranging from 1 - 8 m while House Sparrow nests were at heights of 1 - 5 m. Moult data suggests that although the House Sparrows breed throughout the year, they moult at a particular time of the year when breeding is less common. Grey-headed Sparrows were found to moult mainly from May to September in southern Africa and from June to September in central Africa. In both cases the breeding season extends over a similar period from about October to April/May of the following year. Peak moult periods differed between the House Sparrows and Grey-headed Sparrows. House Sparrows moulted mainly in the first half of the year, and Greyheaded Sparrows in the second six months. The clutch sizes of the two species were similar (mean 3.9 eggs for the House Sparrow and 3.4 for the Grey-headed Sparrow). The clutch size of the House Sparrow varied seasonally and was larger from November to May. The average incubation period for the House Sparrow was 11.5 days and the fledging period 15.4 days. The Grey-headed Sparrow fledging period was 14.7 days. Chick mortality of the House Sparrow at Chikunda farm was attributed to starvation resulting from brood reduction, abandonment, predation, low birth weight, accidental deaths and parasitism by fly larvae. Both Grey-headed and House Sparrows fed their young on insect food. Male House Sparrows fed actively initially, but their contribution declined from about day five onwards. In the Grey-headed Sparrow, both parents fed their young equally throughout the nestling period. House Sparrows fed on the ground near houses; Grey-headed Sparrows fed both on the ground away from houses and in tree canopies. The Grey-headed Sparrow walked as it fed on the ground as opposed to the House sparrow which hopped. Grey-headed Sparrows fed mainly as pairs and singletons while House Sparrows fed as family groups. Larger feeding groups of Grey-headed Sparrows were seen in the northern region at areas where food was plentiful. Where the two sparrows were seen feeding together, there was no direct competition for food. Where individual distance was violated; male House Sparrows displaced Grey-headed Sparrows which landed too close to them. Overall it appears that the distribution of the two species is determined more by their responses to habitat conditions than by interspecific interactions.
- Full Text:
- Authors: Nhlane, Martin Edwin Darwin
- Date: 1997
- Subjects: English sparrow Sparrows
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5654 , http://hdl.handle.net/10962/d1005337
- Description: The House Sparrow Passer domesticus, an introduced species, and the Grey-headed Sparrow Passer griseus, an indigenous species, are sympatric in Malawi. Their distribution in the country and any possible interactions were studied, principally in southern Malawi. A morphological analysis of museum specimens confirmed that grey-headed sparrows in Malawi belong to the Northern Grey-headed Sparrow Passer griseus as distinct from the Southern Grey-headed Sparrow Passer diffusus. This species was widely distributed in the, country in association with human dwellings, both in rural areas as well as urban centres. In the northern region Greyheaded Sparrows were more abundant in the urban centres than rural areas, but in the central and southern regions numbers in the rural and urban areas were more or less the same. In Blantyre City, where they are in sympatry with the House Sparrow, they were found in the low density and industrial areas and were absent from the high density areas. The House Sparrow, arrived in Malawi in 1967 at Chileka in the southern region. Since then it has spread northwards, moving from the southern to the central and northern regions. House Sparrow numbers were found to be progressively larger in the southern region and lowest in the northern region. House Sparrows were found at sites where food was readily available, as in the immediate vicinity of houses. In the central and northern regions they were restricted mainly to urban areas. In the southern region, they occur both in rural and urban areas, probably as a reflection of the larger period of colonization in the south. In the northern region their movement has apparently been restricted by geographical barriers. In Blantyre City Grey-headed Sparrows preferred areas where tree density was high and house density was low, while House Sparrows preferred areas where house density was high and tree density was low. There was a positive correlation between Greyheaded Sparrow numbers and tree density and a negative correlation with house density. House Sparrow abundance was negatively correlated with tree density and positively correlated with house density. Grey-headed Sparrows bred in the rainy season, whereas House Sparrows bred throughout the year. There were differences in nest site selection: Grey-headed Sparrows used artificial structures such as fencing poles, and wooden telephone or electricity poles. The House Sparrow used mostly buildings and nested in crevices, holes in walls and between the walls and rafters. Nest height also differed- Grey-headed Sparrows nested at heights ranging from 1 - 8 m while House Sparrow nests were at heights of 1 - 5 m. Moult data suggests that although the House Sparrows breed throughout the year, they moult at a particular time of the year when breeding is less common. Grey-headed Sparrows were found to moult mainly from May to September in southern Africa and from June to September in central Africa. In both cases the breeding season extends over a similar period from about October to April/May of the following year. Peak moult periods differed between the House Sparrows and Grey-headed Sparrows. House Sparrows moulted mainly in the first half of the year, and Greyheaded Sparrows in the second six months. The clutch sizes of the two species were similar (mean 3.9 eggs for the House Sparrow and 3.4 for the Grey-headed Sparrow). The clutch size of the House Sparrow varied seasonally and was larger from November to May. The average incubation period for the House Sparrow was 11.5 days and the fledging period 15.4 days. The Grey-headed Sparrow fledging period was 14.7 days. Chick mortality of the House Sparrow at Chikunda farm was attributed to starvation resulting from brood reduction, abandonment, predation, low birth weight, accidental deaths and parasitism by fly larvae. Both Grey-headed and House Sparrows fed their young on insect food. Male House Sparrows fed actively initially, but their contribution declined from about day five onwards. In the Grey-headed Sparrow, both parents fed their young equally throughout the nestling period. House Sparrows fed on the ground near houses; Grey-headed Sparrows fed both on the ground away from houses and in tree canopies. The Grey-headed Sparrow walked as it fed on the ground as opposed to the House sparrow which hopped. Grey-headed Sparrows fed mainly as pairs and singletons while House Sparrows fed as family groups. Larger feeding groups of Grey-headed Sparrows were seen in the northern region at areas where food was plentiful. Where the two sparrows were seen feeding together, there was no direct competition for food. Where individual distance was violated; male House Sparrows displaced Grey-headed Sparrows which landed too close to them. Overall it appears that the distribution of the two species is determined more by their responses to habitat conditions than by interspecific interactions.
- Full Text:
Solid state thermal decomposition of amide complexes of nickel(II) chloride
- Nelwamondo, Aubrey Ndifelani
- Authors: Nelwamondo, Aubrey Ndifelani
- Date: 1997
- Subjects: Decomposition (Chemistry) Materials -- Thermal properties Amides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4345 , http://hdl.handle.net/10962/d1005008
- Description: The thermal decompositions of a series of amide complexes of nickel(II) chloride have been studied. Thermochemical, kinetic, structure and solid-state stability correlations have been investigated. Complexes containing homologous amide ligands (L) of the form NiLCℓ₂, Ni₃L₂Cℓ₆, Ni₃LCℓ₆, NiL₂Cℓ₂(2H₂0) and ML₂Cℓ₂ (where M = Ni(II), Co(II) or Cu(II)) have been prepared. Chemical analysis, spectral and thermogravimetric measurements were used to characterize the complexes and their decomposition stoichiometries. Three sets of reactions were identified as yielding stable products in a single step: (i) NiLCℓ₂ (s) → NiCℓ₂ (s) + L (g) (ii) Ni₃LCℓ₆ (s) → 3NiCℓ₂ (s) + 2L (g) (iii) Ni₃LCℓ₆ (s) → 3NiCℓ₂ (s) + L (g) Characterization of the processes in the ML₂Cℓ₂ and NiL₂Cℓ₂(2H₂0) complexes was not straightforward. Reaction enthalpies (ΔH) were determined using DSC. The orders of the reaction onset temperatures (Tc), peak temperatures (Tmax) and ΔHL values for the NiCℓ₂ system were: N-methylacetamide < acetamide < N-methylformamide, suggesting the importance of steric hindrance of the methyl-substituent groups in the amide skeleton. In the Ni₃LCℓ₆, NiL₂Cℓ₂(2H₂0) and ML₂Cℓ₂ systems no simple orders could be deduced. The Te and Tmax sequences obtained from analogous metal(II) chloride complexes indicated that the copper(II) complexes were the least stable. The kinetics of the loss of L from NiLCℓ₂ complexes were investigated using isothermal TG, non-isothermal TG and DSC measurements. The contracting geometry models described the course of the decompositions in the most satisfactory manner. Apparent activation energies ( Ea) were estimated from Arrhenius plots of rate coefficients from: (i) an approximate zero-order relationship, (ii) the contracting-area (R2) and contracting-volume (R3) equations, (iii) a new empirical (B2) expression, (iv) the half-life ( 1/t₀.₅) and (v) the characteristic feature of the rate-time curve ( 1/tmax/2 ). The non-dependence of Ea on the rate equation used supports the reliability of the kinetic parameters. Non-isothermal experiments were analyzed by the Coats-Redfern, the modified BorchardtDaniels and the Kissinger methods. Arrhenius parameters were in keeping with results from the isothermal kinetic measurements. The values of Ea obtained for the NiLCℓ₂ system increased with an increase in basicity of the amide ligands. No straightforward correlation was found between Ea and Te, Tmax, ΔHL or spectral properties.
- Full Text:
- Authors: Nelwamondo, Aubrey Ndifelani
- Date: 1997
- Subjects: Decomposition (Chemistry) Materials -- Thermal properties Amides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4345 , http://hdl.handle.net/10962/d1005008
- Description: The thermal decompositions of a series of amide complexes of nickel(II) chloride have been studied. Thermochemical, kinetic, structure and solid-state stability correlations have been investigated. Complexes containing homologous amide ligands (L) of the form NiLCℓ₂, Ni₃L₂Cℓ₆, Ni₃LCℓ₆, NiL₂Cℓ₂(2H₂0) and ML₂Cℓ₂ (where M = Ni(II), Co(II) or Cu(II)) have been prepared. Chemical analysis, spectral and thermogravimetric measurements were used to characterize the complexes and their decomposition stoichiometries. Three sets of reactions were identified as yielding stable products in a single step: (i) NiLCℓ₂ (s) → NiCℓ₂ (s) + L (g) (ii) Ni₃LCℓ₆ (s) → 3NiCℓ₂ (s) + 2L (g) (iii) Ni₃LCℓ₆ (s) → 3NiCℓ₂ (s) + L (g) Characterization of the processes in the ML₂Cℓ₂ and NiL₂Cℓ₂(2H₂0) complexes was not straightforward. Reaction enthalpies (ΔH) were determined using DSC. The orders of the reaction onset temperatures (Tc), peak temperatures (Tmax) and ΔHL values for the NiCℓ₂ system were: N-methylacetamide < acetamide < N-methylformamide, suggesting the importance of steric hindrance of the methyl-substituent groups in the amide skeleton. In the Ni₃LCℓ₆, NiL₂Cℓ₂(2H₂0) and ML₂Cℓ₂ systems no simple orders could be deduced. The Te and Tmax sequences obtained from analogous metal(II) chloride complexes indicated that the copper(II) complexes were the least stable. The kinetics of the loss of L from NiLCℓ₂ complexes were investigated using isothermal TG, non-isothermal TG and DSC measurements. The contracting geometry models described the course of the decompositions in the most satisfactory manner. Apparent activation energies ( Ea) were estimated from Arrhenius plots of rate coefficients from: (i) an approximate zero-order relationship, (ii) the contracting-area (R2) and contracting-volume (R3) equations, (iii) a new empirical (B2) expression, (iv) the half-life ( 1/t₀.₅) and (v) the characteristic feature of the rate-time curve ( 1/tmax/2 ). The non-dependence of Ea on the rate equation used supports the reliability of the kinetic parameters. Non-isothermal experiments were analyzed by the Coats-Redfern, the modified BorchardtDaniels and the Kissinger methods. Arrhenius parameters were in keeping with results from the isothermal kinetic measurements. The values of Ea obtained for the NiLCℓ₂ system increased with an increase in basicity of the amide ligands. No straightforward correlation was found between Ea and Te, Tmax, ΔHL or spectral properties.
- Full Text:
Regional and local economic development strategies in the Eastern Cape and guidelines for future development
- Nel, E L
- Authors: Nel, E L
- Date: 1997
- Subjects: Community development -- South Africa -- Eastern Cape Case studies Community development Community development -- South Africa -- Eastern Cape Eastern Cape (South Africa) -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4848 , http://hdl.handle.net/10962/d1005524
- Description: Local Economic Development (LED) is an applied economic development strategy which seeks to address site-specific needs through locally appropriate solutions. In this thesis, the faith being accorded to the potential of LED in South Africa is critically examined. The study is based on a detailed examination of the experience of regional development and several emerging cases of LED in the Eastern Cape Province of South Africa. High levels of poverty and unemployment necessitate and justify innovative approaches to address such problems. The thesis examines the potential of LED strategies and identifies applied policy guidelines which can help address the Province's development needs. The theoretical framework of this research is based on a critical examination of international and South African literature dealing with development issues, LED and localities theory. A detailed documentary examination of early LED endeavours and the experience of regional development in the present century was undertaken. Results suggest that LED is not a new concept and that regional development, through its political bias, achieved only limited success. Contemporary examples of LED were identified and examined through detailed site-specific investigations. This was carried out through the use of semistructured interviews supplemented by participatory rural appraisal and questionnaire surveys which served as a means to triangulate the results. The research findings indicate that regional development, as applied in the study area, did not lead to the establishment of a permanent industrial base. In terms of the concept of LED, it is apparent that it has allowed for socio-political empowerment but has only improved economic conditions in the more well-endowed case-studies. In these areas, positive antecedent conditions and the key role played by community supportive nongovernmental organizations is apparent. Despite the limited degree of success which many initiatives attain, the thesis regards LED as a development alternative for areas which are unlikely to draw in external investment. Some of the key contributions of the thesis include the advancement of a refined typology of LED, the development of a research schedule to investigate and assess LED initiatives and the postulation of appropriate development guidelines and theoretical constructs.
- Full Text:
- Authors: Nel, E L
- Date: 1997
- Subjects: Community development -- South Africa -- Eastern Cape Case studies Community development Community development -- South Africa -- Eastern Cape Eastern Cape (South Africa) -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4848 , http://hdl.handle.net/10962/d1005524
- Description: Local Economic Development (LED) is an applied economic development strategy which seeks to address site-specific needs through locally appropriate solutions. In this thesis, the faith being accorded to the potential of LED in South Africa is critically examined. The study is based on a detailed examination of the experience of regional development and several emerging cases of LED in the Eastern Cape Province of South Africa. High levels of poverty and unemployment necessitate and justify innovative approaches to address such problems. The thesis examines the potential of LED strategies and identifies applied policy guidelines which can help address the Province's development needs. The theoretical framework of this research is based on a critical examination of international and South African literature dealing with development issues, LED and localities theory. A detailed documentary examination of early LED endeavours and the experience of regional development in the present century was undertaken. Results suggest that LED is not a new concept and that regional development, through its political bias, achieved only limited success. Contemporary examples of LED were identified and examined through detailed site-specific investigations. This was carried out through the use of semistructured interviews supplemented by participatory rural appraisal and questionnaire surveys which served as a means to triangulate the results. The research findings indicate that regional development, as applied in the study area, did not lead to the establishment of a permanent industrial base. In terms of the concept of LED, it is apparent that it has allowed for socio-political empowerment but has only improved economic conditions in the more well-endowed case-studies. In these areas, positive antecedent conditions and the key role played by community supportive nongovernmental organizations is apparent. Despite the limited degree of success which many initiatives attain, the thesis regards LED as a development alternative for areas which are unlikely to draw in external investment. Some of the key contributions of the thesis include the advancement of a refined typology of LED, the development of a research schedule to investigate and assess LED initiatives and the postulation of appropriate development guidelines and theoretical constructs.
- Full Text:
Ecological interaction between the introduced and native rock-dwelling cichlid fishes of Lake Malawi National Park, Malawi
- Authors: Munthali, Simon Muchina
- Date: 1997
- Subjects: Cichlids -- Nyasa, Lake
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5266 , http://hdl.handle.net/10962/d1005110
- Description: More than twenty years ago, over twenty species of the rock-dwelling cichlid species (Mbuna) were translocated from the northern Lake Malawi, where they are endemic, to Thumbi West Island, Lake Malawi National Park, in the southern part of Lake Malawi. Among these species, Cynotilapia afra, Pseudotropheus callainos and Pseudotropheus tropheops 'red cheek' are strongly territorial, and have increased substantially in number and are widely distributed, particularly in the three to seven metre depth band of the rocky habitats at the Island of Thumbi West. It is feared that the increase in population density of translocated species (hereafter referred to as introduced species) may be at the expense of ecologically equivalent native species which could be eliminated. In this thesis the following key hypotheses have been tested: (i) that the introduced species having originated from a region of Lake Malawi which is generally poor in nutrients and introduced in an area which is richer in nutrients, would cope better than the native species during periods of nutrient scarcity which occur frequently, often seasonally in oligotrophic lakes, such as Lake Malawi; (ii) that the introduced species are fitter than their ecologically equivalent native species in the acquisition of territorial space in which they breed, feed and seek shelter, and (iii) that introduced and native species coexist by utilizing different microhabitats. Results show that: 1. the introduced species, P. callainos and P. tropheops 'red cheek' may have responded positively to enhanced nutrient availability, as they were found to have better condition factors and fecundity indices at Thumbi West Island than at sites of their origin, in the northern lake Malawi. Cynotilapia afra, P. callainos and P. tropheops 'red cheek' also maximise their life-span fecundity by starting to reproduce at relatively smaller size than the native species with which they overlap in microhabitat requirements. Similarly, their breeding peaks precede the breeding peaks of the native species with which they overlap in microhabitat requirements. Consequently, due to priority residence effects, the offspring of introduced species may have a competitive edge in the use of essential resources, e.g., refuge over the offspring of the native species whose peak-recruitment occurs later in the year. 2. There is an overlap between the introduced and native species in their microhabitat requirements. Consequently, interference competition between them for territorial sites occurs. The choice of optimal territory sites is constrained by the fact that females preferentially mate with males that defend significantly smaller holes, or crevices among the rocks, probably as a means of minimizing egg predation during spawning. 3. The population of territorial males of introduced species seems to grow exponentially, depending on the availability of suitable microhabitats, and an equilibrium between them and males of the native species may be reached. Competition for optimal territory sites seems to intensify, once the carrying capacity in a particular area has been reached, and it is at this stage that some territorial males of the introduced and native species with similar microhabitat requirements, e.g., C. afra and P. zebra, or P. tropheops 'red cheek' and its sibling native species, P. tropheops 'orange chest' displace each other. However, it seems unlikely that any of the native species which were compared with the introduced species would be driven to extinction because: (a) there is a considerable interspecific territory turn-over between the introduced and native species that overlap in microhabitat requirements. (b) Even in situations where some of the native species occur in microhabitats that are not of their preference, they occupy patches of suitable sites and are capable of breeding. (c) It has been suggested that since introduced and native species breed throughout the year and are polygamous and have intraspecifically shared paternity, they are capable of fertilizing many gravid females of their own species. Therefore, the population of native species may not be detrimentally limited by the presence of introduced species. (d) The introduced and native Mbuna species that prefer small rocks coexist in the same microhabitats, partly by feeding at different sites with different intensity and they also feed at different heights in the water column. 4. The following studies have been recommended before any management intervention, such as culling is adopted: (i). interaction between the introduced and native species in the shallow and deep rocky habitats; (ii) space utilization and survivorship of juveniles of the introduced and native species; (iii) laboratory studies to confirm the role of different nutrient regimes on the fecundity of Mbuna; (iv) the possibility of hybridization between the introduced and native species; (v) monitoring of population growth and distribution of the introduced species around Thumbi West Island should continue in order to detect their long-term effects on the native species.
- Full Text:
- Authors: Munthali, Simon Muchina
- Date: 1997
- Subjects: Cichlids -- Nyasa, Lake
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5266 , http://hdl.handle.net/10962/d1005110
- Description: More than twenty years ago, over twenty species of the rock-dwelling cichlid species (Mbuna) were translocated from the northern Lake Malawi, where they are endemic, to Thumbi West Island, Lake Malawi National Park, in the southern part of Lake Malawi. Among these species, Cynotilapia afra, Pseudotropheus callainos and Pseudotropheus tropheops 'red cheek' are strongly territorial, and have increased substantially in number and are widely distributed, particularly in the three to seven metre depth band of the rocky habitats at the Island of Thumbi West. It is feared that the increase in population density of translocated species (hereafter referred to as introduced species) may be at the expense of ecologically equivalent native species which could be eliminated. In this thesis the following key hypotheses have been tested: (i) that the introduced species having originated from a region of Lake Malawi which is generally poor in nutrients and introduced in an area which is richer in nutrients, would cope better than the native species during periods of nutrient scarcity which occur frequently, often seasonally in oligotrophic lakes, such as Lake Malawi; (ii) that the introduced species are fitter than their ecologically equivalent native species in the acquisition of territorial space in which they breed, feed and seek shelter, and (iii) that introduced and native species coexist by utilizing different microhabitats. Results show that: 1. the introduced species, P. callainos and P. tropheops 'red cheek' may have responded positively to enhanced nutrient availability, as they were found to have better condition factors and fecundity indices at Thumbi West Island than at sites of their origin, in the northern lake Malawi. Cynotilapia afra, P. callainos and P. tropheops 'red cheek' also maximise their life-span fecundity by starting to reproduce at relatively smaller size than the native species with which they overlap in microhabitat requirements. Similarly, their breeding peaks precede the breeding peaks of the native species with which they overlap in microhabitat requirements. Consequently, due to priority residence effects, the offspring of introduced species may have a competitive edge in the use of essential resources, e.g., refuge over the offspring of the native species whose peak-recruitment occurs later in the year. 2. There is an overlap between the introduced and native species in their microhabitat requirements. Consequently, interference competition between them for territorial sites occurs. The choice of optimal territory sites is constrained by the fact that females preferentially mate with males that defend significantly smaller holes, or crevices among the rocks, probably as a means of minimizing egg predation during spawning. 3. The population of territorial males of introduced species seems to grow exponentially, depending on the availability of suitable microhabitats, and an equilibrium between them and males of the native species may be reached. Competition for optimal territory sites seems to intensify, once the carrying capacity in a particular area has been reached, and it is at this stage that some territorial males of the introduced and native species with similar microhabitat requirements, e.g., C. afra and P. zebra, or P. tropheops 'red cheek' and its sibling native species, P. tropheops 'orange chest' displace each other. However, it seems unlikely that any of the native species which were compared with the introduced species would be driven to extinction because: (a) there is a considerable interspecific territory turn-over between the introduced and native species that overlap in microhabitat requirements. (b) Even in situations where some of the native species occur in microhabitats that are not of their preference, they occupy patches of suitable sites and are capable of breeding. (c) It has been suggested that since introduced and native species breed throughout the year and are polygamous and have intraspecifically shared paternity, they are capable of fertilizing many gravid females of their own species. Therefore, the population of native species may not be detrimentally limited by the presence of introduced species. (d) The introduced and native Mbuna species that prefer small rocks coexist in the same microhabitats, partly by feeding at different sites with different intensity and they also feed at different heights in the water column. 4. The following studies have been recommended before any management intervention, such as culling is adopted: (i). interaction between the introduced and native species in the shallow and deep rocky habitats; (ii) space utilization and survivorship of juveniles of the introduced and native species; (iii) laboratory studies to confirm the role of different nutrient regimes on the fecundity of Mbuna; (iv) the possibility of hybridization between the introduced and native species; (v) monitoring of population growth and distribution of the introduced species around Thumbi West Island should continue in order to detect their long-term effects on the native species.
- Full Text:
The effect of elevated CO₂ on Phaseolus vulgaris L. cv Contender
- Authors: Mjwara, Jabulani Michael
- Date: 1997
- Subjects: Plants -- Effect of carbon dioxide on Kidney bean
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4215 , http://hdl.handle.net/10962/d1003784
- Description: The response of Phaseolus vulgaris L. cv. Contender grown in controlled environmental conditions, at either ambient or elevated (360 and 700 μmol mol ̄¹, respectively) CO₂ concentrations ([CO₂]), was monitored from 10 days after germination (DAG) until the onset of senescence. Elevated CO₂ had a pronounced effect on total plant height (TPH), leaf area (LA), dry weight (DW) accumulation and specific leaf area (SLA). All of these were significantly increased by elevated [CO₂] with the exception of SLA, which was significantly reduced. Except for higher initial relative growth rates (RGR) in CO₂-enriched plants, RGR did not differ significantly between the two CO₂ treatments throughout the remainder of growth period. While growth parameters clearly differed between CO₂ treatments, the effects of CO₂ on many physiological processes including net assimilation rate (NAR), Rubisco activity, and some foliar nutrient concentrations were largely transient. For example, CO₂ enrichment significantly increased NAR, but from 20 DAG onward, NAR declined to levels measured on plants grown under ambient CO₂. Similarly, the decline in both foliar N concentration and Rubisco activity in CO₂-enriched plants after 20 DAG was significantly greater than the decline observed for ambient CO₂ plants. Soluble leaf protein and total chlorophylls (a+b) were also significantly reduced in plants grown under elevated CO₂. Chlorophyll (a/b) ratios increased with time underelevated CO₂, indicating that the rate of decline of chlorophyll b was higher than that of chorophyll α. No significant changes in total carotenoid (x+c) levels were observed in either CO₂ treatment. Under enhanced CO₂, the foliar concentrations of K and Mn were increased significantly, while P, Ca, Fe and Zn were reduced significantly. However, changes in Mg and Cu concentrations were not significant. High CO₂-grown plants also exhibited pronounced leaf discoloration or chlorosis, coupled with a significant reduction in leaf longevity. The levels of non-structural carbohydrates (sucrose, glucose, fructose and starch) and nitrogenous compounds (nitrogen, total soluble proteins and free amino acids) were determined for leaves and developing seeds of P. vulgaris. Leaf tissue of elevated CO₂-grown plants accumulated significantly higher levels of both soluble sugars and starch. Leaf ultrastructure revealed considerable erilargement of starch grain sizes with surface areas more than five times larger compared to those of control plants. No apparent differences in structure and membrane integrity of chloroplasts in both CO₂ treatments were noted. Although ambient CO₂-grown plants had comparatively low levels of non-structural carbohydrates (NSC), they accumulated significantly higher levels of nitrogenous compounds. The levels of NSC were consistently higher in seeds of plants grown under elevated CO₂. In comparison to plants grown at elevated [CO₂], pods and seeds of ambient CO₂-grown plants had significantly larger pools of free amino compounds and N. Stomatal conductance (gs) declined significantly, as expected for plants grown under elevated CO₂. This was accompanied by a decline in transpiration rates (E). Reduced gs and E led to high AlE ratio, which meant improved water use efficiency (WUE) values for CO₂-enriched bean plants. Leaf carbon isotope discrimination (∆) against the heavier isotope of carbon (¹³C), has been used to select for high WUE in C₃ plants. In plants grown at elevated CO₂ concentration, ,1 was significantly reduced. Although ∆ was negatively correlated with WUE in both CO₂ treatments, the correlation was steeper and highly negative for CO₂-enriched plants. These results indicate underlying differences in gas-exchange physiology, including stomatal responses between ambient and elevated CO₂-grown plants. Photosynthetic acclimation was investigated using the response of assimilation to internal carbon dioxide concentration (A/C₁ curves). At early stages of growth, the initial slope of the A/C₁ response curve did not differ with CO₂ treatment. In contrast, CO₂-saturated photosynthetic rate (Amax) was significantly higher in plants grown under elevated versus ambient CO₂ at 15 DAG. However, at subsequent stages of growth both the initial slope and Amax declined in bean plants grown in elevated CO₂. Apparent carboxylation efficiency (ACE, estimated from the initial slope of A/C₁ response) values followed a similar trend and were significantly reduced in CO₂-enriched plants. These results indicate that acclimation or negative adjustment of photosynthesis may have been caused by a combination of both stomatal and biochemical limitations. Bean plants grown under conditions of elevated atmospheric CO₂ flowered 3 to 4 days earlier, and produced significantly more flowers and pods than plants grown at ambient conditions. Plants grown at elevated CO₂ aborted 22 and 20% more flowers and pods, respectively, than plants grown at ambient CO₂. Elevated CO₂ also significantly increased the number of tillers or lateral branches produced by plants, which contributed to a significant increase in pod number and seed yield in these plants. Although plants grown at elevated CO₂ produced on average 8 seeds per pod, while plants grown under ambient CO2 conditions produced 5 seeds per pod, the greater number of seeds was offset by lower seed weights in plants grown under _ elevated CO₂. Thus, despite high seed yield in beans grown under elevated CO₂, the harvest index (HI) did not change significantly between CO₂ treatments.
- Full Text:
- Authors: Mjwara, Jabulani Michael
- Date: 1997
- Subjects: Plants -- Effect of carbon dioxide on Kidney bean
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4215 , http://hdl.handle.net/10962/d1003784
- Description: The response of Phaseolus vulgaris L. cv. Contender grown in controlled environmental conditions, at either ambient or elevated (360 and 700 μmol mol ̄¹, respectively) CO₂ concentrations ([CO₂]), was monitored from 10 days after germination (DAG) until the onset of senescence. Elevated CO₂ had a pronounced effect on total plant height (TPH), leaf area (LA), dry weight (DW) accumulation and specific leaf area (SLA). All of these were significantly increased by elevated [CO₂] with the exception of SLA, which was significantly reduced. Except for higher initial relative growth rates (RGR) in CO₂-enriched plants, RGR did not differ significantly between the two CO₂ treatments throughout the remainder of growth period. While growth parameters clearly differed between CO₂ treatments, the effects of CO₂ on many physiological processes including net assimilation rate (NAR), Rubisco activity, and some foliar nutrient concentrations were largely transient. For example, CO₂ enrichment significantly increased NAR, but from 20 DAG onward, NAR declined to levels measured on plants grown under ambient CO₂. Similarly, the decline in both foliar N concentration and Rubisco activity in CO₂-enriched plants after 20 DAG was significantly greater than the decline observed for ambient CO₂ plants. Soluble leaf protein and total chlorophylls (a+b) were also significantly reduced in plants grown under elevated CO₂. Chlorophyll (a/b) ratios increased with time underelevated CO₂, indicating that the rate of decline of chlorophyll b was higher than that of chorophyll α. No significant changes in total carotenoid (x+c) levels were observed in either CO₂ treatment. Under enhanced CO₂, the foliar concentrations of K and Mn were increased significantly, while P, Ca, Fe and Zn were reduced significantly. However, changes in Mg and Cu concentrations were not significant. High CO₂-grown plants also exhibited pronounced leaf discoloration or chlorosis, coupled with a significant reduction in leaf longevity. The levels of non-structural carbohydrates (sucrose, glucose, fructose and starch) and nitrogenous compounds (nitrogen, total soluble proteins and free amino acids) were determined for leaves and developing seeds of P. vulgaris. Leaf tissue of elevated CO₂-grown plants accumulated significantly higher levels of both soluble sugars and starch. Leaf ultrastructure revealed considerable erilargement of starch grain sizes with surface areas more than five times larger compared to those of control plants. No apparent differences in structure and membrane integrity of chloroplasts in both CO₂ treatments were noted. Although ambient CO₂-grown plants had comparatively low levels of non-structural carbohydrates (NSC), they accumulated significantly higher levels of nitrogenous compounds. The levels of NSC were consistently higher in seeds of plants grown under elevated CO₂. In comparison to plants grown at elevated [CO₂], pods and seeds of ambient CO₂-grown plants had significantly larger pools of free amino compounds and N. Stomatal conductance (gs) declined significantly, as expected for plants grown under elevated CO₂. This was accompanied by a decline in transpiration rates (E). Reduced gs and E led to high AlE ratio, which meant improved water use efficiency (WUE) values for CO₂-enriched bean plants. Leaf carbon isotope discrimination (∆) against the heavier isotope of carbon (¹³C), has been used to select for high WUE in C₃ plants. In plants grown at elevated CO₂ concentration, ,1 was significantly reduced. Although ∆ was negatively correlated with WUE in both CO₂ treatments, the correlation was steeper and highly negative for CO₂-enriched plants. These results indicate underlying differences in gas-exchange physiology, including stomatal responses between ambient and elevated CO₂-grown plants. Photosynthetic acclimation was investigated using the response of assimilation to internal carbon dioxide concentration (A/C₁ curves). At early stages of growth, the initial slope of the A/C₁ response curve did not differ with CO₂ treatment. In contrast, CO₂-saturated photosynthetic rate (Amax) was significantly higher in plants grown under elevated versus ambient CO₂ at 15 DAG. However, at subsequent stages of growth both the initial slope and Amax declined in bean plants grown in elevated CO₂. Apparent carboxylation efficiency (ACE, estimated from the initial slope of A/C₁ response) values followed a similar trend and were significantly reduced in CO₂-enriched plants. These results indicate that acclimation or negative adjustment of photosynthesis may have been caused by a combination of both stomatal and biochemical limitations. Bean plants grown under conditions of elevated atmospheric CO₂ flowered 3 to 4 days earlier, and produced significantly more flowers and pods than plants grown at ambient conditions. Plants grown at elevated CO₂ aborted 22 and 20% more flowers and pods, respectively, than plants grown at ambient CO₂. Elevated CO₂ also significantly increased the number of tillers or lateral branches produced by plants, which contributed to a significant increase in pod number and seed yield in these plants. Although plants grown at elevated CO₂ produced on average 8 seeds per pod, while plants grown under ambient CO2 conditions produced 5 seeds per pod, the greater number of seeds was offset by lower seed weights in plants grown under _ elevated CO₂. Thus, despite high seed yield in beans grown under elevated CO₂, the harvest index (HI) did not change significantly between CO₂ treatments.
- Full Text:
Metabolic responses in melanoma cells to combined nutrient supplementation
- Authors: Midgley, Nicola-Ann
- Date: 1997
- Subjects: Melanoma Tumors -- Growth
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4036 , http://hdl.handle.net/10962/d1004096
- Description: This thesis examined the effect and biochemical mechanism by which combined vitamin E and C supplementation may influence tumour cell growth. The study initially addressed the effect of combined vitamin E succinate and Asc supplementation over a nutritional concentration range (5- 20μg/ml) and (25-50μg/ml) respectively, on the in vitro growth of non-malignant LLCMK and malignant BL6 cells. Supplementation of BL6 and LLCMK cells with combined vitamin E succinate and ascorbic acid, resulted in no significant increasing or decreasing trend in LLCMK cell growth, while in BL6 cells a significant decrease in cell growth was observed at all combined vitamin concentrations. It has been suggested that these vitamins may act synergistically to inhibit tumour cell growth through their antioxidant properties in quenching free radicals and lipid peroxidation and furthermore through their modulation of the activities of various enzymes and metabolites in the eicosanoid pathway. This study consequently investigated the effects of combined vitamin E succinate and ascorbic acid supplementation on these parameters. Throughout this study, emphasis was placed on the BL6 melanoma cells, as combined vitamin E succinate and ascorbic acid supplementation did not significantly affect growth or levels of secondary metabolites in the non-malignant LLCMK cells. Combined vitamin E succinate and ascorbic acid supplementation of BL6 cells resulted in a marked but non significant increase in free radical and a significant increase in lipid peroxidation levels. This prooxidant effect was accompanied by a significant decrease in BL6 cell growth, suggesting that the growth inhibitory effects of combined vitainin E succinate and ascorbic acid on BL6 cells in vitro was not mediated through their synergistic antioxidant properties. Vitamin E succinate is a nonphysiological antioxidant in its esterified form, hence cleavage of the succinate group must occur in order for ascorbic acid to interact with the free alcohol, vitamin E. The inability of combined vitamin E succinate and ascorbic acid to reduce free radicals and lipid peroxidation levels within BL6 cells may not be due to their ineffectiveness as antioxidants but rather the presence of other contributing factors which influence the oxidation state within the BL6 cells. Vitamin E is believed to modulate membrane-bound enzymes through membrane stabilization. Furthermore, the stabilizing effect of vitamin E may be enhanced by the ascorbic acid-sparing effect of vitamin E. Hence, this study investigated the effect of combined vitamin E succinate and ascorbic acid in modulating the activity of various enzymes and secondary messengers in the eicosanoid pathway. Supplementation with combined vitamin E succinate (5-20μg/ml) and ascorbic acid (25-50μg/ml) resulted in significant increases in phospholipase A₂, 5-lipoxygenase, cyclooxygenase and adenyl ate cyclase activity, with a significant decrease in BL6 cell growth. The possible synergistic action of these vitamins in terms of modulating membrane-bound enzymes was further substantiated by uptake and cellular distribution studies. Vitamin E succinate and vitamin E in the membrane fraction increased significantly compared to control cultures, while ascorbic acid levels were significantly higher in the stroma fraction when compared to membrane fractions. Consequently, another factor accounting for increased activities of phospholipase A2, 5-lipoxygenase and adenylate cyclase activities as a result of vitamin supplementation in BL6 cells may be an increased availability of Ca²+. Supplementation of BL6 cells with combined vitamin E succinate and ascorbic acid resulted in significant increases in intracellular Ca²+ levels at all combined vitamin groups. Furthermore, this increase in intracellular Ca²+ was positively correlated with cl1anges of the above-mentioned enzyme activities. Within the eicosanoid pathway, the rate of prostaglandin synthesis is regulated by phospholipase A₂ activity and arachidonic acid release, and the net prostaglandin production is dependent on cyclooxygenase activity, hence the effects of combined vitamin E succinate and ascorbic acid on arachidonic acid composition and prostaglandin production within BL6 cells was determined. The percentage arachidonic acid composition of the BL6 cells was elevated and inversely related to cell growth following combined vitamin E succinate and ascorbic acid supplementation. Prostaglandin E₂ and prostaglandin I₂ levels increased significantly, while those of prostaglandin D2 and prostaglandin F₂α increased markedly following supplementation of combined vitamin E succinate and ascorbic acid. These increases in prostaglandin levels were inversely related to BL6 cell growth, suggesting that the prostaglandins were involved in negative regulation of BL6 cell growth. When comparing the levels of prostaglandins, prostaglandin E2 levels were significantly higher when compared to prostaglandin D₂, prostaglandin F₂α and prostaglandin I₂ suggesting that vitamin E₂ succinate and ascorbic acid effects were mediated primarily through an increase in prostaglandin E2. Hence, prostaglandin E2 levels in combined vitamin E succinate and ascorbic acid appeared to be dependent on the amount of precursor present and the activity of its synthetic enzymes. This was confirmed when BL6 cells were supplemented with arachidonic acid. Arachidonic acid had an inhibitory effect on BL6 cell growth and also stimulated prostaglandin E₂ production. Prostaglandin E₂ levels are in turn believed to modulate adenylate cyclase activity in BL6 cells, hence it is reasonable to conclude that adenylate cyclase activity is dependent on prostaglandin E₂ levels. Combined vitamin E succinate and Asc supplementation to BL6 cells resulted in significant increases in adenyl ate cyclase and cyclic adenosine monophosphate, which again correlated with a significant decrease in cell growth. As cyclic adenosine monophosphate has a regulatory role in the cell cycle this study suggested that the effect of combined vitamin E succinate and ascorbic acid supplementation was mediated through the final effect provided by the second messenger, cyclic adenosine monophosphate. This was confirmed when BL6 cells were supplemented with dexamethasone, a phospholipase A₂ inhibitor. This treatment rsulted in combined vitamin E succinate and ascorbic acid having no inhibitory effect on BL6 cell growth. Cyclooxygenase activity, prostaglandin E₂ levels, adenylate cyclase activity and cyclic adenosine monophosphate levels were significantly lower in dexamethasone-treated cells compared to non-treated dexamethasone cultures. The reason for the increased free radical and lipid peroxidation levels in BL6 cells was further investigated. Cyclooxygenase enzymes are believed to generate free radical species during catalytic activity. Analysis of free radical and lipid peroxidation levels following supplementation with dexamethasone revealed markedly lower free radical and significantly lower lipid peroxidation levels in comparison with control cultures and non dexamethasone-treated cultures. These results suggest that the observed increases in free radical and lipid peroxidation levels in BL6 cells supplemented with combined vitamin E succinate and ascorbic acid were indirectly due to the increase in cyclooxygenase activity in these cells.
- Full Text:
- Authors: Midgley, Nicola-Ann
- Date: 1997
- Subjects: Melanoma Tumors -- Growth
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4036 , http://hdl.handle.net/10962/d1004096
- Description: This thesis examined the effect and biochemical mechanism by which combined vitamin E and C supplementation may influence tumour cell growth. The study initially addressed the effect of combined vitamin E succinate and Asc supplementation over a nutritional concentration range (5- 20μg/ml) and (25-50μg/ml) respectively, on the in vitro growth of non-malignant LLCMK and malignant BL6 cells. Supplementation of BL6 and LLCMK cells with combined vitamin E succinate and ascorbic acid, resulted in no significant increasing or decreasing trend in LLCMK cell growth, while in BL6 cells a significant decrease in cell growth was observed at all combined vitamin concentrations. It has been suggested that these vitamins may act synergistically to inhibit tumour cell growth through their antioxidant properties in quenching free radicals and lipid peroxidation and furthermore through their modulation of the activities of various enzymes and metabolites in the eicosanoid pathway. This study consequently investigated the effects of combined vitamin E succinate and ascorbic acid supplementation on these parameters. Throughout this study, emphasis was placed on the BL6 melanoma cells, as combined vitamin E succinate and ascorbic acid supplementation did not significantly affect growth or levels of secondary metabolites in the non-malignant LLCMK cells. Combined vitamin E succinate and ascorbic acid supplementation of BL6 cells resulted in a marked but non significant increase in free radical and a significant increase in lipid peroxidation levels. This prooxidant effect was accompanied by a significant decrease in BL6 cell growth, suggesting that the growth inhibitory effects of combined vitainin E succinate and ascorbic acid on BL6 cells in vitro was not mediated through their synergistic antioxidant properties. Vitamin E succinate is a nonphysiological antioxidant in its esterified form, hence cleavage of the succinate group must occur in order for ascorbic acid to interact with the free alcohol, vitamin E. The inability of combined vitamin E succinate and ascorbic acid to reduce free radicals and lipid peroxidation levels within BL6 cells may not be due to their ineffectiveness as antioxidants but rather the presence of other contributing factors which influence the oxidation state within the BL6 cells. Vitamin E is believed to modulate membrane-bound enzymes through membrane stabilization. Furthermore, the stabilizing effect of vitamin E may be enhanced by the ascorbic acid-sparing effect of vitamin E. Hence, this study investigated the effect of combined vitamin E succinate and ascorbic acid in modulating the activity of various enzymes and secondary messengers in the eicosanoid pathway. Supplementation with combined vitamin E succinate (5-20μg/ml) and ascorbic acid (25-50μg/ml) resulted in significant increases in phospholipase A₂, 5-lipoxygenase, cyclooxygenase and adenyl ate cyclase activity, with a significant decrease in BL6 cell growth. The possible synergistic action of these vitamins in terms of modulating membrane-bound enzymes was further substantiated by uptake and cellular distribution studies. Vitamin E succinate and vitamin E in the membrane fraction increased significantly compared to control cultures, while ascorbic acid levels were significantly higher in the stroma fraction when compared to membrane fractions. Consequently, another factor accounting for increased activities of phospholipase A2, 5-lipoxygenase and adenylate cyclase activities as a result of vitamin supplementation in BL6 cells may be an increased availability of Ca²+. Supplementation of BL6 cells with combined vitamin E succinate and ascorbic acid resulted in significant increases in intracellular Ca²+ levels at all combined vitamin groups. Furthermore, this increase in intracellular Ca²+ was positively correlated with cl1anges of the above-mentioned enzyme activities. Within the eicosanoid pathway, the rate of prostaglandin synthesis is regulated by phospholipase A₂ activity and arachidonic acid release, and the net prostaglandin production is dependent on cyclooxygenase activity, hence the effects of combined vitamin E succinate and ascorbic acid on arachidonic acid composition and prostaglandin production within BL6 cells was determined. The percentage arachidonic acid composition of the BL6 cells was elevated and inversely related to cell growth following combined vitamin E succinate and ascorbic acid supplementation. Prostaglandin E₂ and prostaglandin I₂ levels increased significantly, while those of prostaglandin D2 and prostaglandin F₂α increased markedly following supplementation of combined vitamin E succinate and ascorbic acid. These increases in prostaglandin levels were inversely related to BL6 cell growth, suggesting that the prostaglandins were involved in negative regulation of BL6 cell growth. When comparing the levels of prostaglandins, prostaglandin E2 levels were significantly higher when compared to prostaglandin D₂, prostaglandin F₂α and prostaglandin I₂ suggesting that vitamin E₂ succinate and ascorbic acid effects were mediated primarily through an increase in prostaglandin E2. Hence, prostaglandin E2 levels in combined vitamin E succinate and ascorbic acid appeared to be dependent on the amount of precursor present and the activity of its synthetic enzymes. This was confirmed when BL6 cells were supplemented with arachidonic acid. Arachidonic acid had an inhibitory effect on BL6 cell growth and also stimulated prostaglandin E₂ production. Prostaglandin E₂ levels are in turn believed to modulate adenylate cyclase activity in BL6 cells, hence it is reasonable to conclude that adenylate cyclase activity is dependent on prostaglandin E₂ levels. Combined vitamin E succinate and Asc supplementation to BL6 cells resulted in significant increases in adenyl ate cyclase and cyclic adenosine monophosphate, which again correlated with a significant decrease in cell growth. As cyclic adenosine monophosphate has a regulatory role in the cell cycle this study suggested that the effect of combined vitamin E succinate and ascorbic acid supplementation was mediated through the final effect provided by the second messenger, cyclic adenosine monophosphate. This was confirmed when BL6 cells were supplemented with dexamethasone, a phospholipase A₂ inhibitor. This treatment rsulted in combined vitamin E succinate and ascorbic acid having no inhibitory effect on BL6 cell growth. Cyclooxygenase activity, prostaglandin E₂ levels, adenylate cyclase activity and cyclic adenosine monophosphate levels were significantly lower in dexamethasone-treated cells compared to non-treated dexamethasone cultures. The reason for the increased free radical and lipid peroxidation levels in BL6 cells was further investigated. Cyclooxygenase enzymes are believed to generate free radical species during catalytic activity. Analysis of free radical and lipid peroxidation levels following supplementation with dexamethasone revealed markedly lower free radical and significantly lower lipid peroxidation levels in comparison with control cultures and non dexamethasone-treated cultures. These results suggest that the observed increases in free radical and lipid peroxidation levels in BL6 cells supplemented with combined vitamin E succinate and ascorbic acid were indirectly due to the increase in cyclooxygenase activity in these cells.
- Full Text:
The oral-style South African short story in English A.W. Drayson to H.C. Bosman
- Authors: MacKenzie, Craig
- Date: 1997
- Subjects: Short stories, South African (English) -- History and criticism
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2228 , http://hdl.handle.net/10962/d1002271
- Description: This study is concerned with a particular kind of short story in South African English literature - a kind of story variously called the fireside tale, tall tale, yarn, skaz narrative, frame narrative, or (the term used in this study), the 'oral-sty Ie story.' This kind of story is characterised by the use of an internal narrator (a fictional narrator or storyteller figure), the cadences of his or her speaking voice, and a 'reporting' frame narrator. Stories by A. W. Drayson, Frederick Boyle, J. Forsyth Ingram, W. C. Scully, Percy FitzPatrick, Ernest Glanville, Perceval Gibbon, Francis Carey Slater, Pauline Smith, Aegidius Jean Blignaut and Herman Charles Bosman form the principal body of primary sources examined in this study. The Bakhtinian notion of "simple" and "parodistic" skaz narratives is deployed to analyse the increasing complexity to be discerned in the works by these writers, which roughly span the 100 years from the middle of the nineteenth century to the middle of the present century. A "simple" use of the skaz narrative is evident in the early or 'ur-South African' oral-style story, represented here by Drayson, Boyle and Ingram. With Scully and FitzPatrick the form is still used 'artlessly,' although the beginnings of a greater self-consciousness can be discerned. The' Abe Pike' tales by Glanville introduce a more complex use of the fictional narrator, a process taken a step further by Gibbon in his 'Vrouw Grobelaar' tales. With the latter, in particular, the complex or "parodistic" skaz narrative makes its advent in South African literature. The oral-style stories of Slater and Smith are largely a regression to the ear lier form, although there are aspects of their stories which anticipate Bosman. With Blignaut and Bosman, however, the South African oral-style story comes into its own. In their Hottentot Ruiter and Oom Schalk Lourens characters is invested all the complexity and 'double-voicedness' that was latent, and largely dormant, in the earlier oral-style narratives. Through Blignaut, and Bosman in particular, the South African oral-style story achieves its most economical, sophisticated and successful form of expression. The study concludes by looking briefly at the use of an oral style in short stories by black South African writers and argues that their stories are not, formally speaking, to be categorised alongside those by the other~ writers examined. The oral-style story, the study concludes, achieved its apogee in Bosman's Oom Schalk Lourens sequence and went into sharp decline after Bosman's death in 1951.
- Full Text:
- Authors: MacKenzie, Craig
- Date: 1997
- Subjects: Short stories, South African (English) -- History and criticism
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2228 , http://hdl.handle.net/10962/d1002271
- Description: This study is concerned with a particular kind of short story in South African English literature - a kind of story variously called the fireside tale, tall tale, yarn, skaz narrative, frame narrative, or (the term used in this study), the 'oral-sty Ie story.' This kind of story is characterised by the use of an internal narrator (a fictional narrator or storyteller figure), the cadences of his or her speaking voice, and a 'reporting' frame narrator. Stories by A. W. Drayson, Frederick Boyle, J. Forsyth Ingram, W. C. Scully, Percy FitzPatrick, Ernest Glanville, Perceval Gibbon, Francis Carey Slater, Pauline Smith, Aegidius Jean Blignaut and Herman Charles Bosman form the principal body of primary sources examined in this study. The Bakhtinian notion of "simple" and "parodistic" skaz narratives is deployed to analyse the increasing complexity to be discerned in the works by these writers, which roughly span the 100 years from the middle of the nineteenth century to the middle of the present century. A "simple" use of the skaz narrative is evident in the early or 'ur-South African' oral-style story, represented here by Drayson, Boyle and Ingram. With Scully and FitzPatrick the form is still used 'artlessly,' although the beginnings of a greater self-consciousness can be discerned. The' Abe Pike' tales by Glanville introduce a more complex use of the fictional narrator, a process taken a step further by Gibbon in his 'Vrouw Grobelaar' tales. With the latter, in particular, the complex or "parodistic" skaz narrative makes its advent in South African literature. The oral-style stories of Slater and Smith are largely a regression to the ear lier form, although there are aspects of their stories which anticipate Bosman. With Blignaut and Bosman, however, the South African oral-style story comes into its own. In their Hottentot Ruiter and Oom Schalk Lourens characters is invested all the complexity and 'double-voicedness' that was latent, and largely dormant, in the earlier oral-style narratives. Through Blignaut, and Bosman in particular, the South African oral-style story achieves its most economical, sophisticated and successful form of expression. The study concludes by looking briefly at the use of an oral style in short stories by black South African writers and argues that their stories are not, formally speaking, to be categorised alongside those by the other~ writers examined. The oral-style story, the study concludes, achieved its apogee in Bosman's Oom Schalk Lourens sequence and went into sharp decline after Bosman's death in 1951.
- Full Text:
Healing stories of the unconscious: past-life imagery in transpersonal psychotherapy
- Authors: Knight, Zelda Gillian
- Date: 1997
- Subjects: Transpersonal psychotherapy Unconscious Reincarnation therapy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3004 , http://hdl.handle.net/10962/d1002513
- Description: Theoretically this thesis was grounded in the discourse of transpersonal psychology and the related discourse of transpersonal feminism. The focus was on a particular category of transpersonal phenomena - past-life experiences. These experiences were viewed from a poetic and therapeutic perspective as being healing stories of the unconscious that served to articulate psychological and spiritual realities of the human psyche within both the personal and the collective unconscious. They were thus not questioned in regard to their literal occurrence. The central aims of this thesis were to (a) document and faithfully describe a participant's past-life experiences that occurred during selected psychotherapy sessions, (b) engage in a hermeneutic dialogue between the participant's past-life experiences and contemporary transpersonal literature, and, in so doing, to evaluate and extend existing theory, (c) uncover the archetypal significance of past-life experience and its relationship to the re-emerging Feminine within patriarchal culture and, finally, (d) show how the past-life stories and images contribute to the process of inner healing and transformation, a process termed 'spiritual emergence'. The research was a phenomenological-hermeneutic case study, comprising the selection of eight consecutive psychotherapy sessions in which nine past-life experiences were identified. These sessions were reduced to narrative synopses, and a hermeneutically grounded thematic analysis of a total of six past-life themes were explicated. Principle conclusions reached were that past-life stories and images contribute to the process of spiritual emergence and empowerment as well as to the re-emergence of the Feminine consciousness. Moreover, as healing stories of the unconscious, these past-life experiences can be understood as expressions of the collective struggle with unresolved archetypal forces within the collective psyche, as well as echoes of personal conflicts and dilemmas from the individual unconscious.
- Full Text:
- Authors: Knight, Zelda Gillian
- Date: 1997
- Subjects: Transpersonal psychotherapy Unconscious Reincarnation therapy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3004 , http://hdl.handle.net/10962/d1002513
- Description: Theoretically this thesis was grounded in the discourse of transpersonal psychology and the related discourse of transpersonal feminism. The focus was on a particular category of transpersonal phenomena - past-life experiences. These experiences were viewed from a poetic and therapeutic perspective as being healing stories of the unconscious that served to articulate psychological and spiritual realities of the human psyche within both the personal and the collective unconscious. They were thus not questioned in regard to their literal occurrence. The central aims of this thesis were to (a) document and faithfully describe a participant's past-life experiences that occurred during selected psychotherapy sessions, (b) engage in a hermeneutic dialogue between the participant's past-life experiences and contemporary transpersonal literature, and, in so doing, to evaluate and extend existing theory, (c) uncover the archetypal significance of past-life experience and its relationship to the re-emerging Feminine within patriarchal culture and, finally, (d) show how the past-life stories and images contribute to the process of inner healing and transformation, a process termed 'spiritual emergence'. The research was a phenomenological-hermeneutic case study, comprising the selection of eight consecutive psychotherapy sessions in which nine past-life experiences were identified. These sessions were reduced to narrative synopses, and a hermeneutically grounded thematic analysis of a total of six past-life themes were explicated. Principle conclusions reached were that past-life stories and images contribute to the process of spiritual emergence and empowerment as well as to the re-emergence of the Feminine consciousness. Moreover, as healing stories of the unconscious, these past-life experiences can be understood as expressions of the collective struggle with unresolved archetypal forces within the collective psyche, as well as echoes of personal conflicts and dilemmas from the individual unconscious.
- Full Text: