The polysaccharides of Opuntia ficus-indica (L.) Mill. and Opuntia aurantiaca Lindl.
- Authors: McGarvie, Donald
- Date: 1977
- Subjects: Polysaccharides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4438 , http://hdl.handle.net/10962/d1007597
- Description: The partially acetylated acidic mucilage isolated from the cactus Opuntia ficus-indica consists of a highly branched molecule containing D-galactose, L-arabinose, D-xylose, D-galacturonic acid and L-rhamnose in the ratio 2.9:6.6:3.8:1.3 : 1.0. A partial hydrolysis study led to the isolation of fourteen oligosaccharides and a degraded polysaccharide. Periodate oxidation of the degraded polysaccharide and methylation analysis of the degraded and reduced degraded polysaccharides led to the proposal of a structure for the degraded polysaccharide consisting of a chain of alternating α-l,4-D-galactopyranosyluronic acid and β-1,2-Lrhamnopyranosyl units with branches consisting of short chains of β-1,6-D-galactopyranosyl units Iinked to C-3 of the rhamnose residues. An insight into the nature of the peripheral side-chains was obtained by methylation studies of the oligosaccharides and the native polysaccharide. The majority of the side-chains are terminated by D-xylopyranosyl and L-arabinofuranosyl units while there is a small proportion of D-galactopyranosyl end-groups. The remaining units of the peripheral chains consists of mainly 1,3- and 1,5-1 inked L-arabinofuranosyl units. The acidic polysaccharide isolated from the jointed cactus Opuntia aurantiaca contains D-galactose, L-arabinose, D-xylose, D-galacturonic acid and L-rhamnose in the ratio 5.9:5 .5:3.4: 1. 2:1.0 . A partial hydrolysis study revealed a similar series of galactose containing oligosaccharides as was identified from the mucilage of Opuntia ficus-indica. The degraded polysaccharide isolated from the partial hydrolysate was methylated and a possible structure proposed for the repeating unit. Methylation studies of the native polysaccharide indicated a polysaccharide with a more complex structure than that for the mucilage of Opuntia ficus-indica.
- Full Text:
- Date Issued: 1977
- Authors: McGarvie, Donald
- Date: 1977
- Subjects: Polysaccharides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4438 , http://hdl.handle.net/10962/d1007597
- Description: The partially acetylated acidic mucilage isolated from the cactus Opuntia ficus-indica consists of a highly branched molecule containing D-galactose, L-arabinose, D-xylose, D-galacturonic acid and L-rhamnose in the ratio 2.9:6.6:3.8:1.3 : 1.0. A partial hydrolysis study led to the isolation of fourteen oligosaccharides and a degraded polysaccharide. Periodate oxidation of the degraded polysaccharide and methylation analysis of the degraded and reduced degraded polysaccharides led to the proposal of a structure for the degraded polysaccharide consisting of a chain of alternating α-l,4-D-galactopyranosyluronic acid and β-1,2-Lrhamnopyranosyl units with branches consisting of short chains of β-1,6-D-galactopyranosyl units Iinked to C-3 of the rhamnose residues. An insight into the nature of the peripheral side-chains was obtained by methylation studies of the oligosaccharides and the native polysaccharide. The majority of the side-chains are terminated by D-xylopyranosyl and L-arabinofuranosyl units while there is a small proportion of D-galactopyranosyl end-groups. The remaining units of the peripheral chains consists of mainly 1,3- and 1,5-1 inked L-arabinofuranosyl units. The acidic polysaccharide isolated from the jointed cactus Opuntia aurantiaca contains D-galactose, L-arabinose, D-xylose, D-galacturonic acid and L-rhamnose in the ratio 5.9:5 .5:3.4: 1. 2:1.0 . A partial hydrolysis study revealed a similar series of galactose containing oligosaccharides as was identified from the mucilage of Opuntia ficus-indica. The degraded polysaccharide isolated from the partial hydrolysate was methylated and a possible structure proposed for the repeating unit. Methylation studies of the native polysaccharide indicated a polysaccharide with a more complex structure than that for the mucilage of Opuntia ficus-indica.
- Full Text:
- Date Issued: 1977
5-hydroxytryptamine and sexual behaviour in rhesus monkeys
- Authors: Gradwell, Peter Bertram
- Date: 1976
- Subjects: Rhesus monkey -- Behavior Sexual behavior in animals
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3210 , http://hdl.handle.net/10962/d1012086
- Description: Selective inhibition of 5-hydroxytryptamine by parachlorophenylalanine (PCPA) is able to restore sexual receptivity in female rhesus monkeys made unreceptive by bilateral adrenalectomy. PCPA in the doses used reduces the levels of 5-hydroxyindoleacetic acid (5HIAA) in the cerebrospinal fluid to 40 per cent of the normal oestradioltreated condition. Both the increased sexual receptivity and the lowered 5HIAA levels "in the CSF are in turn reversed by 5-hydroxytryptophal (5HTP), the irrmediate precursor of 5HT and the substance whose synthesis is inhibited by PCPA. 5HTP on its own reduces sexual receptivity and increases 5HIAA levels in the CSF of ovariectomised, oestradiol-treated (but otherwise intact) female rhesus monkeys. A substance other than an adrenal androgen has therefore been shown to restore sexual receptivity in adrenalectomised female monkeys . Testosterone propionate and oestradiol benzoate both lower the turnover rates of 5HT in the brains of ovariectomised female monkeys, as measured by the 2 hour probenecid test. Taken together, these findings suggest that adrenal androgens could act on specific sites in the female monkey brain via 5HT-containing neural systems, to control (or at least influence) sexual receptivity. All the results of administering oestradiol to ovariectomised monkeys in these experiments are consistent with the established roles of this hormone in female sexual attractiveness and in the gonadotrophin- controlling systems of the hypothalamo-hypophyseal axis. In contrast to these findings on 5HT and sexual receptivity in female monkeys, no clear role for 5HT- containing neural systems could be demonstrated in the grooming, aggressive or social behaviours of female monkeys. No clear role for 5HT could be demonstrated in the refractory period following ejaculation in male monkeys , or when testosterone replacement is given to castrated male monkeys.
- Full Text:
- Date Issued: 1976
- Authors: Gradwell, Peter Bertram
- Date: 1976
- Subjects: Rhesus monkey -- Behavior Sexual behavior in animals
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3210 , http://hdl.handle.net/10962/d1012086
- Description: Selective inhibition of 5-hydroxytryptamine by parachlorophenylalanine (PCPA) is able to restore sexual receptivity in female rhesus monkeys made unreceptive by bilateral adrenalectomy. PCPA in the doses used reduces the levels of 5-hydroxyindoleacetic acid (5HIAA) in the cerebrospinal fluid to 40 per cent of the normal oestradioltreated condition. Both the increased sexual receptivity and the lowered 5HIAA levels "in the CSF are in turn reversed by 5-hydroxytryptophal (5HTP), the irrmediate precursor of 5HT and the substance whose synthesis is inhibited by PCPA. 5HTP on its own reduces sexual receptivity and increases 5HIAA levels in the CSF of ovariectomised, oestradiol-treated (but otherwise intact) female rhesus monkeys. A substance other than an adrenal androgen has therefore been shown to restore sexual receptivity in adrenalectomised female monkeys . Testosterone propionate and oestradiol benzoate both lower the turnover rates of 5HT in the brains of ovariectomised female monkeys, as measured by the 2 hour probenecid test. Taken together, these findings suggest that adrenal androgens could act on specific sites in the female monkey brain via 5HT-containing neural systems, to control (or at least influence) sexual receptivity. All the results of administering oestradiol to ovariectomised monkeys in these experiments are consistent with the established roles of this hormone in female sexual attractiveness and in the gonadotrophin- controlling systems of the hypothalamo-hypophyseal axis. In contrast to these findings on 5HT and sexual receptivity in female monkeys, no clear role for 5HT- containing neural systems could be demonstrated in the grooming, aggressive or social behaviours of female monkeys. No clear role for 5HT could be demonstrated in the refractory period following ejaculation in male monkeys , or when testosterone replacement is given to castrated male monkeys.
- Full Text:
- Date Issued: 1976
A study of factors affecting precision in atomic absorption spectrometry
- Roos, Johannes Tielman Hofmeyr
- Authors: Roos, Johannes Tielman Hofmeyr
- Date: 1976
- Subjects: Atomic absorption spectroscopy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4490 , http://hdl.handle.net/10962/d1013080
- Description: 1. The effect of deviations from Beer's law on the precision of atomic absorption analysis has been examined from a theoretical point of view, and a function has been derived which makes it possible to evaluate quantitatively the effect of calibration curvature on the precision of analysis. The influence of incomplete sample volatilization on calibration curvature has been briefly investigated. 2. Possible error sources in atomic absorption spectrometry have been classified according to the "error function" (i.e., the dependence, upon transmittance T, of the uncertainty dT in a given transmittance measurement) with which they are associated. The magnitude of the contribution from each component function to the overall error function has been evaluated quantitatively, and it has been shown that the major component in nearly every case examined is that associated with the dynamic nature of the flame. Concentration ranges for optimum precision are suggested. 3. The effect of varying instrumental parameters on precision has been investigated, and generalized conditions for best precision have been ascertained. 4. The effect of an initial solvent extraction step on the precision of atomic absorption has been investigated for the elements copper and lead. It is shown that solvent extraction may be used to improve both the analytical sensitivity and the precision of analysis when very low concentrations of metal are determined. 5. The precision of analytical methods involving atomic absorption spectrometry has been studied, and the standard deviations compared with those obtained for the analysis of similar samples by means of a variety of other methods of analysis, both instrumental and classical.
- Full Text:
- Date Issued: 1976
- Authors: Roos, Johannes Tielman Hofmeyr
- Date: 1976
- Subjects: Atomic absorption spectroscopy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4490 , http://hdl.handle.net/10962/d1013080
- Description: 1. The effect of deviations from Beer's law on the precision of atomic absorption analysis has been examined from a theoretical point of view, and a function has been derived which makes it possible to evaluate quantitatively the effect of calibration curvature on the precision of analysis. The influence of incomplete sample volatilization on calibration curvature has been briefly investigated. 2. Possible error sources in atomic absorption spectrometry have been classified according to the "error function" (i.e., the dependence, upon transmittance T, of the uncertainty dT in a given transmittance measurement) with which they are associated. The magnitude of the contribution from each component function to the overall error function has been evaluated quantitatively, and it has been shown that the major component in nearly every case examined is that associated with the dynamic nature of the flame. Concentration ranges for optimum precision are suggested. 3. The effect of varying instrumental parameters on precision has been investigated, and generalized conditions for best precision have been ascertained. 4. The effect of an initial solvent extraction step on the precision of atomic absorption has been investigated for the elements copper and lead. It is shown that solvent extraction may be used to improve both the analytical sensitivity and the precision of analysis when very low concentrations of metal are determined. 5. The precision of analytical methods involving atomic absorption spectrometry has been studied, and the standard deviations compared with those obtained for the analysis of similar samples by means of a variety of other methods of analysis, both instrumental and classical.
- Full Text:
- Date Issued: 1976
Agnation, alternative structures, and the individual in Chopi society
- Authors: Webster, D J
- Date: 1976
- Subjects: Chopi (African people) , Ethnology -- Mozambique
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2122 , http://hdl.handle.net/10962/d1013288
- Full Text:
- Date Issued: 1976
- Authors: Webster, D J
- Date: 1976
- Subjects: Chopi (African people) , Ethnology -- Mozambique
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2122 , http://hdl.handle.net/10962/d1013288
- Full Text:
- Date Issued: 1976
Drummer Hodge : the poetry of the Anglo-Boer War (1899-1902)
- Authors: Van Wyk Smith, Malvern
- Date: 1976
- Subjects: War poetry -- History and criticism South African War, 1899-1902 -- Literature and the war South African War, 1899-1902 -- Art and the war
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2252 , http://hdl.handle.net/10962/d1003919
- Description: From Preface: This is not a history of the Boer War; nor is it an exclusively literary study of the poetry of that war. If the work that follows has to be defined generically at all, it may be called an exercise in cultural history. It attempts to assess the impact of a particular war on the literary culture, especially the poetry, of both the participants and the observers, whether in South Africa, in Britain and the rest of the English-speaking world, or in Europe. An assumption made throughout this study is that war poetry is not only verse written by men who are or have been under fire. Just as 'War poetry is not to be confused with political, polemical, or patriotic verse, although it can contain elements of all of these, so it is also the work of observers at home as much as that of soldiers at the front. It follows that I have not allowed myself the academic luxury of selecting, on the basis of literary merit only, a handful of outstanding war poems for rigorous analysis and discussion. "Doggerel can express the heart" wrote one of these late-Victorian soldierly versifiers, and I have roamed widely in the attempt to assemble the material which, I believe, records the full range of the impact that the Boer War made not only on Briton and Boer, but on the worId at large. A major thesis of this study is that the Boer War marked the clear emergence of the kind of war poetry which we have come to associate almost exclusively with the First World War. Poems in the style and spirit of "The Charge of the Light Brigade" were written in profusion, but the work which serves as this study's masthead, Hardy's "Drummer Hodge," clearly has --like many of its contemporaries-- more in common with Owen's verse than with Tennyson's. The reasons for the appearance of such poetry are discussed in Chapter 1; the rest of the book provides the evidence of it.
- Full Text:
- Date Issued: 1976
- Authors: Van Wyk Smith, Malvern
- Date: 1976
- Subjects: War poetry -- History and criticism South African War, 1899-1902 -- Literature and the war South African War, 1899-1902 -- Art and the war
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2252 , http://hdl.handle.net/10962/d1003919
- Description: From Preface: This is not a history of the Boer War; nor is it an exclusively literary study of the poetry of that war. If the work that follows has to be defined generically at all, it may be called an exercise in cultural history. It attempts to assess the impact of a particular war on the literary culture, especially the poetry, of both the participants and the observers, whether in South Africa, in Britain and the rest of the English-speaking world, or in Europe. An assumption made throughout this study is that war poetry is not only verse written by men who are or have been under fire. Just as 'War poetry is not to be confused with political, polemical, or patriotic verse, although it can contain elements of all of these, so it is also the work of observers at home as much as that of soldiers at the front. It follows that I have not allowed myself the academic luxury of selecting, on the basis of literary merit only, a handful of outstanding war poems for rigorous analysis and discussion. "Doggerel can express the heart" wrote one of these late-Victorian soldierly versifiers, and I have roamed widely in the attempt to assemble the material which, I believe, records the full range of the impact that the Boer War made not only on Briton and Boer, but on the worId at large. A major thesis of this study is that the Boer War marked the clear emergence of the kind of war poetry which we have come to associate almost exclusively with the First World War. Poems in the style and spirit of "The Charge of the Light Brigade" were written in profusion, but the work which serves as this study's masthead, Hardy's "Drummer Hodge," clearly has --like many of its contemporaries-- more in common with Owen's verse than with Tennyson's. The reasons for the appearance of such poetry are discussed in Chapter 1; the rest of the book provides the evidence of it.
- Full Text:
- Date Issued: 1976
Some problems of dialect lexicography with particular reference to the preparation of a draft of an illustrative, experientially categorised Dictionary of South African English
- Authors: Branford, Jean
- Date: 1976
- Subjects: English language -- Provincialisms -- South Africa -- Dictionaries English language -- South Africa English language -- Lexicography -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3626 , http://hdl.handle.net/10962/d1009689
- Description: This dissertation consists in essence of an experiment and a commentary upon it. The text which constitutes Part II is a lexicographical experiment incorporating some features and treatments not usual in lexicography, and Part I consists of a discussion of the problems encountered, principles applied and procedures followed. Neither the matter nor the manner of the experiment, however, lends itself in the present state of our knowledge to the fully impersonal objectivity that is often claimed for the experiments in the physical sciences. At the same time every effort has been made to establish an unbiased record of the data and to maintain a certain methodological consistency. The main experimental feature of Part II is that it is an attempt to combine an orthodox, alphabetical dictionary with an experiential categorisation of the vocabulary, without repeating the entire data for each type of treatment. This has been done by means of a series of numbered, classified word-lists with a limited subject index as a guide to their use. The entries themselves, instead of being repeated in the order of their classification, are numbered according to the category or categories to which the word defined belongs. It can then, by means of its number(s), be found in its own lexical or experiential set (or sets) in the categorised section. This part of the work might be better described as lexicology rather than lexicography but does, I think, prove itself to be a useful adjunct to the A - Z lexicon proper. Apart from the detailed categorisation system the text contains three features not normally included in dictionaries of small compass: illustrative quotations, etymologies and a number of tentative parallels between South African and other varieties of English by means of cross-references to items of similar or related meaning or form in the English of Australia, Canada, the U.S.A., Hong Kong, Anglo-India and other 'overseas' English speech communities. Introduction, p. 1.
- Full Text:
- Date Issued: 1976
- Authors: Branford, Jean
- Date: 1976
- Subjects: English language -- Provincialisms -- South Africa -- Dictionaries English language -- South Africa English language -- Lexicography -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3626 , http://hdl.handle.net/10962/d1009689
- Description: This dissertation consists in essence of an experiment and a commentary upon it. The text which constitutes Part II is a lexicographical experiment incorporating some features and treatments not usual in lexicography, and Part I consists of a discussion of the problems encountered, principles applied and procedures followed. Neither the matter nor the manner of the experiment, however, lends itself in the present state of our knowledge to the fully impersonal objectivity that is often claimed for the experiments in the physical sciences. At the same time every effort has been made to establish an unbiased record of the data and to maintain a certain methodological consistency. The main experimental feature of Part II is that it is an attempt to combine an orthodox, alphabetical dictionary with an experiential categorisation of the vocabulary, without repeating the entire data for each type of treatment. This has been done by means of a series of numbered, classified word-lists with a limited subject index as a guide to their use. The entries themselves, instead of being repeated in the order of their classification, are numbered according to the category or categories to which the word defined belongs. It can then, by means of its number(s), be found in its own lexical or experiential set (or sets) in the categorised section. This part of the work might be better described as lexicology rather than lexicography but does, I think, prove itself to be a useful adjunct to the A - Z lexicon proper. Apart from the detailed categorisation system the text contains three features not normally included in dictionaries of small compass: illustrative quotations, etymologies and a number of tentative parallels between South African and other varieties of English by means of cross-references to items of similar or related meaning or form in the English of Australia, Canada, the U.S.A., Hong Kong, Anglo-India and other 'overseas' English speech communities. Introduction, p. 1.
- Full Text:
- Date Issued: 1976
Stratigraphy and sedimentology of the Cape and Karoo Sequences in the Eastern Cape Province
- Authors: Johnson, M R
- Date: 1976
- Subjects: Sediments (Geology) -- South Africa -- Eastern Cape Geology, Stratigraphic Sedimentology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5004 , http://hdl.handle.net/10962/d1005617
- Description: The Cape Supergroup (Sequence) comprises three groups, embracing a total of twenty-three formations, with a maximum combined thickness of approximately 8 km. The Table Mountain Group consists of medium-grained (occasionally fine- or coarse-grained), "clean", ultra-quartzose sandstone plus subordinate fine-grained, "dirty", subfeldspathic to feldspathic sandstone, mudrock, and rhythmitite. Average total thickness is about 3000 m. The Bokkeveld Group is composed of mudrock, rhythmitite and subordinate subfeldspathic to feldspathic sandstone (generally fine-grained and "dirty"), with a maximum total thickness of over 3000 m. The Witteberg Group comprises fine- to medium-grained ultra-quartzose sandstone, icaceous streaky rhythmitite, mudrock, and one thin diamictite unit; total thickness is about 1700 m. The strata belonging to the Cape Supergroup appear to have been largely deposited under marine conditions in environments ranging from outer shelf to beach. Deltaic deposits are,however, common in the upper part of the Bokkeveld Group and the Witteberg Group, while the main sandstone units in the upper third of the Table Mountain Group may have accumulated on a coastal alluvial plain. Deposition took place in a basin elongated in an east-west direction, with the palaeoslope inclined towards the south. Palaeocurrents were generally directed down the palaeoslope, but westerly 1 transport directions parallel to the palaeostrike and presumed shoreline are present in both the Table Mountain and Witteberg Groups. I The sedimentary rocks o~ the Karoo Sequence are subdivided into two groups (containing a total of eleven formations) and four ungrouped formations. Using the maximum thicknesses of the individual formations, a combined total thickness of about 12 km can be calculated. The sequence commences with the Dwyka Tillite, a 700-m-thick diamictite unit. The overlying Ecca Group consists of "varved" rhythmitite, dark, massive, fine- to very fine-grained ultra-lithofeldspathic sandstone and subordinate mudrock with a total thickness of 2000 - 3000 m. The Beaufort Group is composed of thick mudstone layers alternating with thinner fine-grained ultra-lithofeldspathic, lithofeldspathic and lithic sandstones, with the exception of the Katberg Formation which consists largely of sandstone. Fining-upward cycles are ubiquitous, while red mudstone is com~on, especially in the upper half of the group. A maximum thickness of about 6000 m was obtained in the East London area. The Molteno Formation Consists of up to 600 m of alternating fine- to coarse-grained sublithic sandstones (frequently pebbly) and grey mudstones, generally forming finingupward cycles. The Elliot Formation (up to 500 m thick) consists of red and grey mUdstones and subordinate fine-grained lithofeldspathic sandstones arranged in fining-upward cycles. The bulk of the Clarens Sandstone consists of very fine-grained massive (occasionally cross-bedded) sandstone, with a maximum thickness of 300 m. The Drakensberg Group, consisting of up to 1200 m of basalt with some pyroclastic intercalations near the base, caps the Karoo sedimentary succession. The deposition of the Dwyka Tillite by glacier action coincided with a major change from the generally shallow marine conditions which characterised the sedimentation of the Cape Supergroup (with the source area located on the craton to the north of the basin) to a deep linear trough receiving clastic sediments from a source area situated south and south-east of the basin. The Ecca Group,the lower half of which is characterised by the presence of "proximal" turbidite sandstones, records the gradual infilling of this basin, with deltaic conditions developing in the upper part of the group in the western half of the study area (i.e. in the Waterford Formation). The overlying strata were virtually all deposited under fluviatile conditions, the chief exceptions being a stratigraphic interval within the lower half of the Beaufort Group which appears to have formed in a large body of water, a~d the aeolian Clarens Sandstone. The fluviatile sediments were all deposite1 by rivers flowin~ towards the north and nort~-west, while the Clarens Sandstone was laid down by winds blowing from the west. The Ecca and Beaufort Group sandstones are characterised by a high rock fragment content with "felsit ic" gra ins being a prc;>minent constituent. This, together with the relative abundance of quartzfeldspar porphyry pebbles in the Katberg Sandstone unit (Beaufort Group) near East London, indicates that volcanic material probably formed a prominent part of the post-Dwyka Karoo provenance.
- Full Text:
- Date Issued: 1976
- Authors: Johnson, M R
- Date: 1976
- Subjects: Sediments (Geology) -- South Africa -- Eastern Cape Geology, Stratigraphic Sedimentology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5004 , http://hdl.handle.net/10962/d1005617
- Description: The Cape Supergroup (Sequence) comprises three groups, embracing a total of twenty-three formations, with a maximum combined thickness of approximately 8 km. The Table Mountain Group consists of medium-grained (occasionally fine- or coarse-grained), "clean", ultra-quartzose sandstone plus subordinate fine-grained, "dirty", subfeldspathic to feldspathic sandstone, mudrock, and rhythmitite. Average total thickness is about 3000 m. The Bokkeveld Group is composed of mudrock, rhythmitite and subordinate subfeldspathic to feldspathic sandstone (generally fine-grained and "dirty"), with a maximum total thickness of over 3000 m. The Witteberg Group comprises fine- to medium-grained ultra-quartzose sandstone, icaceous streaky rhythmitite, mudrock, and one thin diamictite unit; total thickness is about 1700 m. The strata belonging to the Cape Supergroup appear to have been largely deposited under marine conditions in environments ranging from outer shelf to beach. Deltaic deposits are,however, common in the upper part of the Bokkeveld Group and the Witteberg Group, while the main sandstone units in the upper third of the Table Mountain Group may have accumulated on a coastal alluvial plain. Deposition took place in a basin elongated in an east-west direction, with the palaeoslope inclined towards the south. Palaeocurrents were generally directed down the palaeoslope, but westerly 1 transport directions parallel to the palaeostrike and presumed shoreline are present in both the Table Mountain and Witteberg Groups. I The sedimentary rocks o~ the Karoo Sequence are subdivided into two groups (containing a total of eleven formations) and four ungrouped formations. Using the maximum thicknesses of the individual formations, a combined total thickness of about 12 km can be calculated. The sequence commences with the Dwyka Tillite, a 700-m-thick diamictite unit. The overlying Ecca Group consists of "varved" rhythmitite, dark, massive, fine- to very fine-grained ultra-lithofeldspathic sandstone and subordinate mudrock with a total thickness of 2000 - 3000 m. The Beaufort Group is composed of thick mudstone layers alternating with thinner fine-grained ultra-lithofeldspathic, lithofeldspathic and lithic sandstones, with the exception of the Katberg Formation which consists largely of sandstone. Fining-upward cycles are ubiquitous, while red mudstone is com~on, especially in the upper half of the group. A maximum thickness of about 6000 m was obtained in the East London area. The Molteno Formation Consists of up to 600 m of alternating fine- to coarse-grained sublithic sandstones (frequently pebbly) and grey mudstones, generally forming finingupward cycles. The Elliot Formation (up to 500 m thick) consists of red and grey mUdstones and subordinate fine-grained lithofeldspathic sandstones arranged in fining-upward cycles. The bulk of the Clarens Sandstone consists of very fine-grained massive (occasionally cross-bedded) sandstone, with a maximum thickness of 300 m. The Drakensberg Group, consisting of up to 1200 m of basalt with some pyroclastic intercalations near the base, caps the Karoo sedimentary succession. The deposition of the Dwyka Tillite by glacier action coincided with a major change from the generally shallow marine conditions which characterised the sedimentation of the Cape Supergroup (with the source area located on the craton to the north of the basin) to a deep linear trough receiving clastic sediments from a source area situated south and south-east of the basin. The Ecca Group,the lower half of which is characterised by the presence of "proximal" turbidite sandstones, records the gradual infilling of this basin, with deltaic conditions developing in the upper part of the group in the western half of the study area (i.e. in the Waterford Formation). The overlying strata were virtually all deposited under fluviatile conditions, the chief exceptions being a stratigraphic interval within the lower half of the Beaufort Group which appears to have formed in a large body of water, a~d the aeolian Clarens Sandstone. The fluviatile sediments were all deposite1 by rivers flowin~ towards the north and nort~-west, while the Clarens Sandstone was laid down by winds blowing from the west. The Ecca and Beaufort Group sandstones are characterised by a high rock fragment content with "felsit ic" gra ins being a prc;>minent constituent. This, together with the relative abundance of quartzfeldspar porphyry pebbles in the Katberg Sandstone unit (Beaufort Group) near East London, indicates that volcanic material probably formed a prominent part of the post-Dwyka Karoo provenance.
- Full Text:
- Date Issued: 1976
The Late Cenozoic history and palaeoenvironments of the coastal margin of the south-western Cape Province, South Africa
- Tankard, Anthony James Tempest
- Authors: Tankard, Anthony James Tempest
- Date: 1976
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:21068 , http://hdl.handle.net/10962/6257
- Description: This thesis examines the Late Cenozoic history and palaeoenvironments of the coastal margin between Elands Bay on the west coast and Die Kelders on the south coast. This study is introduced with a detailed discussion of eustatic sea level oscillation. The history of the existing ice sheets, sea floor spreading, isotopic composition changes of the oceans, and isostatic responses of the crust to varying loads are reviewed with regard to their bearing on sea level changes. A detailed account of the Neogene stratigraphy of the south-western Cape Province is presented. The Middle to early Late Miocene Saldanha Formation is characterised by shallow marine phosphatic sandstone and phosphorite. It is thought to have been deposited in a warm transgressive sea. The Pliocene Varswater Formation was deposited during a secondary transgression induced by.seaward tilting of the coastal margin during a time of worldwide regression. The Varswater Formation is characterised by pelletal phosphorites. It includes marine, estuarine, and fluvial facies. The estuarine sands and peats contain a rich fossil mammal fauna. Depositional environments of the Pelletal Phosphorite Member are examined by means of conventional grain size analysis to show that deposition took place on a shallow sublittoral platform dominated on the outer edge by a breaker-bar. Accretion of the breaker-bar to form a barrier-island allowed the development of an estuarine complex on the leeward side. Post-depositional diagenetic changes were examined by means of scanning electron microscopy. A detailed account of the petrology and geochemistry of the phosphorite and pelletal phosphorite is presented. The apatite mineral is a carbonate fluorapatite. It is concluded that the phosphorite is related to upwelling of phosphorus-rich waters.
- Full Text:
- Date Issued: 1976
- Authors: Tankard, Anthony James Tempest
- Date: 1976
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:21068 , http://hdl.handle.net/10962/6257
- Description: This thesis examines the Late Cenozoic history and palaeoenvironments of the coastal margin between Elands Bay on the west coast and Die Kelders on the south coast. This study is introduced with a detailed discussion of eustatic sea level oscillation. The history of the existing ice sheets, sea floor spreading, isotopic composition changes of the oceans, and isostatic responses of the crust to varying loads are reviewed with regard to their bearing on sea level changes. A detailed account of the Neogene stratigraphy of the south-western Cape Province is presented. The Middle to early Late Miocene Saldanha Formation is characterised by shallow marine phosphatic sandstone and phosphorite. It is thought to have been deposited in a warm transgressive sea. The Pliocene Varswater Formation was deposited during a secondary transgression induced by.seaward tilting of the coastal margin during a time of worldwide regression. The Varswater Formation is characterised by pelletal phosphorites. It includes marine, estuarine, and fluvial facies. The estuarine sands and peats contain a rich fossil mammal fauna. Depositional environments of the Pelletal Phosphorite Member are examined by means of conventional grain size analysis to show that deposition took place on a shallow sublittoral platform dominated on the outer edge by a breaker-bar. Accretion of the breaker-bar to form a barrier-island allowed the development of an estuarine complex on the leeward side. Post-depositional diagenetic changes were examined by means of scanning electron microscopy. A detailed account of the petrology and geochemistry of the phosphorite and pelletal phosphorite is presented. The apatite mineral is a carbonate fluorapatite. It is concluded that the phosphorite is related to upwelling of phosphorus-rich waters.
- Full Text:
- Date Issued: 1976
A contribution to the understanding of the ethology of the cichlids of Southern Africa
- Authors: Ribbink, Anthony J
- Date: 1975
- Subjects: Cichlids -- Africa, Southern , Fishes -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5889 , http://hdl.handle.net/10962/d1013369
- Description: Allopatric populations of Pseudocrenilabrus philander were found to differ in male breeding coloration. These colours are described and attention is drawn to the possibility that male coloration might prevent population hybridisation. Behaviour of the allopatric populations was studied in the laboratory. The execution of behaviour patterns of the various populations was found to be indistinguishable, and very similar to that of Haplochromis. Behaviour of P. philander was recorded in three morphologically different natural water bodies. Diurnal activities were found to follow a set rhythm. Though P. philander was shown to have adapted to a variety of habitats they retained their behavioural Components in an unchanged form. Field and laboratory observations are used to propose a behavioural mechanism for the natural regulation of population density. Although aggression between conspecific territorial Sarot herodon mossambicus habituated so that neighbours could live close to one another, it was found that P.philander are intolerant of their neighbours and are consequently forced apart. Though the execution of behaviour patterns was apparently identical for all populations of P. philander, field observations indicated that the frequency of performance differed. An experimental procedure was established to quantitatively compare the behaviour of various populations. As a result of comparative and choice chamber investigations, it is suggested that one of the four populations of P. philander would be unlikely to hybridise with the others if they were to become sympatric. These popUlations would be kept apart because of colour and behavioural differences. Differences of egg-size, fry-size, developmental and interbrood periods were also found, providing further evidence of incipient speciation. Evolutionary divergence of the allopatric populations of P. philander is discussed against a background of geological and geographical evidence. Consideration is given to the role of male coloration, ethological barriers and the importance of ethological differences to taxonomy. Behavioural evidence suggests that P. philander has closer affinities with the haplochromids than was initially realised. It is concluded that the clinal populations of P. philander should not be taxonomically separated, and it is argued that it would be premature to give the Kuruman population an elevated taxonomic status.
- Full Text:
- Date Issued: 1975
- Authors: Ribbink, Anthony J
- Date: 1975
- Subjects: Cichlids -- Africa, Southern , Fishes -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5889 , http://hdl.handle.net/10962/d1013369
- Description: Allopatric populations of Pseudocrenilabrus philander were found to differ in male breeding coloration. These colours are described and attention is drawn to the possibility that male coloration might prevent population hybridisation. Behaviour of the allopatric populations was studied in the laboratory. The execution of behaviour patterns of the various populations was found to be indistinguishable, and very similar to that of Haplochromis. Behaviour of P. philander was recorded in three morphologically different natural water bodies. Diurnal activities were found to follow a set rhythm. Though P. philander was shown to have adapted to a variety of habitats they retained their behavioural Components in an unchanged form. Field and laboratory observations are used to propose a behavioural mechanism for the natural regulation of population density. Although aggression between conspecific territorial Sarot herodon mossambicus habituated so that neighbours could live close to one another, it was found that P.philander are intolerant of their neighbours and are consequently forced apart. Though the execution of behaviour patterns was apparently identical for all populations of P. philander, field observations indicated that the frequency of performance differed. An experimental procedure was established to quantitatively compare the behaviour of various populations. As a result of comparative and choice chamber investigations, it is suggested that one of the four populations of P. philander would be unlikely to hybridise with the others if they were to become sympatric. These popUlations would be kept apart because of colour and behavioural differences. Differences of egg-size, fry-size, developmental and interbrood periods were also found, providing further evidence of incipient speciation. Evolutionary divergence of the allopatric populations of P. philander is discussed against a background of geological and geographical evidence. Consideration is given to the role of male coloration, ethological barriers and the importance of ethological differences to taxonomy. Behavioural evidence suggests that P. philander has closer affinities with the haplochromids than was initially realised. It is concluded that the clinal populations of P. philander should not be taxonomically separated, and it is argued that it would be premature to give the Kuruman population an elevated taxonomic status.
- Full Text:
- Date Issued: 1975
A dielectric and spectroscopic study of molecular association in solutions of alcohols
- Authors: Campbell, Colin
- Date: 1975
- Subjects: Alcohols , Dielectrics , Spectrum analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4454 , http://hdl.handle.net/10962/d1010424
- Description: This study is concerned with the association characteristics of solutions of alcohols in some non-polar solvents. The permittivities at 2 MHz and 25°C for solutions of the straight chain octanols in cyclohexane, carbon tetrachloride and benzene have been examined over the entire solute concentration range, with particular attention -1 being paid to the range below 0.1 molℓ⁻¹ By applying the Kirkwood- Fröhlich equation to these data, the apparent dipole moments of the alcohols as a function of concentration have been evaluated. These concentration dependencies have been correlated with infrared absorption results on the same systems to provide information on the sizes and configurations of the proposed hydrogen bonded multimers. It is concluded that, at very low solute concentrations, the alcohol molecules exist as monomers; but with increasing concentration, two types of hydrogen bonded multimers are formed, the first (at low concentrations) being of high dipole moment and the second (at higher concentrations) being of low dipole moment. At high concentrations, the molecules associate to form a three-dimensional network. Attempts have been made to determine equilibrium parameters for molecular models which are consistent with the qualitative understanding of the association behaviour. These parameters were obtained by applying least-squares, curve-fitting techniques to the low concentration permittivity data. A similar investigation has been conducted on solutions of 2,3,4-trimethyl-3-pentanol in the same solvents. The steric hindrance around the hydroxyl group of this alcohol modifies the association behaviour so that a three-dimensional network does not form at high solute concentrations. Proton magnetic resonance chemical shifts for the hydroxyl proton of this alcohol in carbon tetrachloride solutions have been measured. Attempts have also been made to determine equilibrium parameters which describe formation of the hydrogen bonded multimers. To extend this study to include solutes other than octanols, similar experiments have been conducted on solutions of t-butanol in hexadecane, a system which has recently been investigated by other workers using different experimental techniques. The association behaviour of this system is qualitatively similar to that of the straight-chain octanols. The combination of permittivity and infrared measurements, although proving extremely powerful in interpreting the association characteristics of dilute alcohol solutions, is less adequate at high solute concentrations. Attention was therefore directed towards dielectric relaxation and viscosity studies to investigate concentrated solutions. The relaxation times at 20°C of the low frequency dispersion have been measured for solutions of 1-propanol, 1-butanol, 1-hexanol, 1-octanol and 1-decanol in cyclohexane using time domain reflectometry techniques. Similar measurements have also been made on solutions of 1-butanol and 1-octanol in carbon tetrachloride and in benzene. The concentration dependence of the viscosities of certain of these systems has also been examined in an independent study. The ratio of the dielectric relaxation time to the viscosity, the "reduced relaxation time", is qualitatively similar for each system studied. This similarity leads to an explanation of the molecular process responsible for the low frequency dispersion in terms of the proximity of the hydroxyl groups in concentrated alcohol solutions and the fraction of the groups which are not involved in hydrogen bonding.
- Full Text:
- Date Issued: 1975
- Authors: Campbell, Colin
- Date: 1975
- Subjects: Alcohols , Dielectrics , Spectrum analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4454 , http://hdl.handle.net/10962/d1010424
- Description: This study is concerned with the association characteristics of solutions of alcohols in some non-polar solvents. The permittivities at 2 MHz and 25°C for solutions of the straight chain octanols in cyclohexane, carbon tetrachloride and benzene have been examined over the entire solute concentration range, with particular attention -1 being paid to the range below 0.1 molℓ⁻¹ By applying the Kirkwood- Fröhlich equation to these data, the apparent dipole moments of the alcohols as a function of concentration have been evaluated. These concentration dependencies have been correlated with infrared absorption results on the same systems to provide information on the sizes and configurations of the proposed hydrogen bonded multimers. It is concluded that, at very low solute concentrations, the alcohol molecules exist as monomers; but with increasing concentration, two types of hydrogen bonded multimers are formed, the first (at low concentrations) being of high dipole moment and the second (at higher concentrations) being of low dipole moment. At high concentrations, the molecules associate to form a three-dimensional network. Attempts have been made to determine equilibrium parameters for molecular models which are consistent with the qualitative understanding of the association behaviour. These parameters were obtained by applying least-squares, curve-fitting techniques to the low concentration permittivity data. A similar investigation has been conducted on solutions of 2,3,4-trimethyl-3-pentanol in the same solvents. The steric hindrance around the hydroxyl group of this alcohol modifies the association behaviour so that a three-dimensional network does not form at high solute concentrations. Proton magnetic resonance chemical shifts for the hydroxyl proton of this alcohol in carbon tetrachloride solutions have been measured. Attempts have also been made to determine equilibrium parameters which describe formation of the hydrogen bonded multimers. To extend this study to include solutes other than octanols, similar experiments have been conducted on solutions of t-butanol in hexadecane, a system which has recently been investigated by other workers using different experimental techniques. The association behaviour of this system is qualitatively similar to that of the straight-chain octanols. The combination of permittivity and infrared measurements, although proving extremely powerful in interpreting the association characteristics of dilute alcohol solutions, is less adequate at high solute concentrations. Attention was therefore directed towards dielectric relaxation and viscosity studies to investigate concentrated solutions. The relaxation times at 20°C of the low frequency dispersion have been measured for solutions of 1-propanol, 1-butanol, 1-hexanol, 1-octanol and 1-decanol in cyclohexane using time domain reflectometry techniques. Similar measurements have also been made on solutions of 1-butanol and 1-octanol in carbon tetrachloride and in benzene. The concentration dependence of the viscosities of certain of these systems has also been examined in an independent study. The ratio of the dielectric relaxation time to the viscosity, the "reduced relaxation time", is qualitatively similar for each system studied. This similarity leads to an explanation of the molecular process responsible for the low frequency dispersion in terms of the proximity of the hydroxyl groups in concentrated alcohol solutions and the fraction of the groups which are not involved in hydrogen bonding.
- Full Text:
- Date Issued: 1975
Accident liability and primary process thinking : a study in ego psychology
- Authors: Nell, Oelrich
- Date: 1975
- Subjects: Automobile drivers -- Psychology , Ego (Psychology) , Traffic safety -- South Africa -- Psychological aspects , Liability for traffic accidents -- South Africa , Traffic accidents -- South Africa -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3220 , http://hdl.handle.net/10962/d1012950
- Description: Serious efforts must be made to combat the high accident rate on the roads in the Republic of South Africa. The same spirit which exists in the field of medicine when there are evidences that an epidemic is beginning to take its toll, must come into being amongst those who are concerned with traffic and its problems. The public itself needs to become more aware of road safety. Many associations and institutions have been created to assist with the inculcation of the road safety ideal. The National Road Safety Council, which creates opportunities for investigation into aspects of road safety must continue to expand its sphere of influence. The research project contained on the following pages represents an attempt to contribute to the human factor in traffic safety, particularly by making a closer study of the driver of the motor vehicle. The writer is concerned with the carnage that is caused by road accidents. His previous study of personality and particularly of the projective techniques of assessment has prompted a consideration of the problem.
- Full Text:
- Date Issued: 1975
- Authors: Nell, Oelrich
- Date: 1975
- Subjects: Automobile drivers -- Psychology , Ego (Psychology) , Traffic safety -- South Africa -- Psychological aspects , Liability for traffic accidents -- South Africa , Traffic accidents -- South Africa -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3220 , http://hdl.handle.net/10962/d1012950
- Description: Serious efforts must be made to combat the high accident rate on the roads in the Republic of South Africa. The same spirit which exists in the field of medicine when there are evidences that an epidemic is beginning to take its toll, must come into being amongst those who are concerned with traffic and its problems. The public itself needs to become more aware of road safety. Many associations and institutions have been created to assist with the inculcation of the road safety ideal. The National Road Safety Council, which creates opportunities for investigation into aspects of road safety must continue to expand its sphere of influence. The research project contained on the following pages represents an attempt to contribute to the human factor in traffic safety, particularly by making a closer study of the driver of the motor vehicle. The writer is concerned with the carnage that is caused by road accidents. His previous study of personality and particularly of the projective techniques of assessment has prompted a consideration of the problem.
- Full Text:
- Date Issued: 1975
From Frontier to Midlands : a history of the Graaff-Reinet district, 1786-1910
- Authors: Smith, Kenneth Wyndham
- Date: 1975
- Subjects: Graaff-Reinet (South Africa) -- History , South Africa -- Economic conditions -- History , South Africa -- Politics and government -- History , Frontier and pioneer life -- Graaff-Reinet
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2615 , http://hdl.handle.net/10962/d1013242
- Description: The study of local history in South Africa is still in its infancy and has not been accorded the same recognition as elsewhere. There is no convenient manual to guide the would-be local historian of the Cape. There are few models that provide an insight into the main problems encountered by the local historian of a Cape community. In such local histories as exist, attention has been focussed predominantly on the foundation and physical growth of towns, the naming of streets, the establishment of schools and hospitals. Many of these accounts were written for publicity purposes or to commemorate the founding of towns. Although there is no history of the Dutch Reformed Church in Graaff-Reinet, the history of local congregations of the Dutch Reformed Church has generally been well covered in the form of Gedenkboeke and other studies. These frequently have a particular relevance as many towns such as Burgersdorp and Colesberg were founded as a result of the initiative of the church. Preface.
- Full Text:
- Date Issued: 1975
- Authors: Smith, Kenneth Wyndham
- Date: 1975
- Subjects: Graaff-Reinet (South Africa) -- History , South Africa -- Economic conditions -- History , South Africa -- Politics and government -- History , Frontier and pioneer life -- Graaff-Reinet
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2615 , http://hdl.handle.net/10962/d1013242
- Description: The study of local history in South Africa is still in its infancy and has not been accorded the same recognition as elsewhere. There is no convenient manual to guide the would-be local historian of the Cape. There are few models that provide an insight into the main problems encountered by the local historian of a Cape community. In such local histories as exist, attention has been focussed predominantly on the foundation and physical growth of towns, the naming of streets, the establishment of schools and hospitals. Many of these accounts were written for publicity purposes or to commemorate the founding of towns. Although there is no history of the Dutch Reformed Church in Graaff-Reinet, the history of local congregations of the Dutch Reformed Church has generally been well covered in the form of Gedenkboeke and other studies. These frequently have a particular relevance as many towns such as Burgersdorp and Colesberg were founded as a result of the initiative of the church. Preface.
- Full Text:
- Date Issued: 1975
Interactions between steroidal anti-inflammatory agents and collagen
- Authors: Kanfer, Isadore
- Date: 1975
- Subjects: Anti-inflammatory agents Collagen
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3849 , http://hdl.handle.net/10962/d1012620
- Description: Much research has been done on the formation of fibrils from solutions of soluble collagen in vitro in order to gain some knowledge of the mechanisms which may occur in vivo. The in vitro formation of fibres from solutions of collagen has been shown to be extremely sensitive to the nature of the solution environment and the presence of added chemical compounds, and thus constitutes an interesting system for the study of collagen-small molecule inter actions. The present study is concerned with the effects of various corticosteroid drugs, used medicinally as anti-inflammatory agents, on collagen in solution. As these corticosteroids are administered to reduce inflammation in conditions such as rheumatoid arthritis and a host of other pathological conditions in which collagen is implicated, this work has been undertaken in order to establish and charac teri ze any binding mechanisms which may be involved. Furthermore, the corticosteroid drugs available commercially in pure form as the free base or as the water-soluble ester salts offer an interesting range of structural and stereochemical variants for the study of their reaction with a complex and biologically important protein molecule such as collagen. A great deal of research on drug- protein interactions (Goldstein, 1949; Meyer and Guttman, 1968a) and more specifically, steroid-protein interactions have been reported over the years (Daughaday, 1959; Sandberg et al., 1966; Villee and Engel, 1961; Westphal , 1971). Comprehensive reports, however, on steroid-collagen interactions in vitro are conspicuously absent from modern scientific literature although relatively superficial accounts have been published (Menczel and Maibach, 1972; Eik-Nes et al., 1954). Although work involving the above has appeared relating specifically to the effects of steroids on collagen biosynthesis both in vivo and in vitro there have been minimal accounts of steroid-collagen interactions tailored to characterize the binding at the molecular level. The effect of corticosteroids on the metabolism of connective tissue has also received special attention (Asboe-Hansen, 1959; Kivirikko, 1953; Nakagawa and Tsurufuji, 1972). Recently, Uitto et al. (1972) reported the effects of several anti-inflammatory corticosteroids on collagen biosynthesis in vitro, whilst Aalto and Kulonen (1972) reported the effects of several antirheumatic drugs on the synthesis of collagen and other proteins in vitro. The interactions between collagen and certain drugs has also been briefly reviewed (Chvapil, 1967). Much data also exists on the binding of a wide range of small molecules and ions with serum albumin (Steinhardt and Reynolds, 1969; Scatchard, 1949; Klotz, 1950). Serum albumin, being specialized for a very general transport function and apparently designed for the purpose of combining with a large range of small molecules, has a proportion of possible reactive sites 'buried' within the molecule itself because of its folded conformation. In addition, serum albumin shows a high degree of cooperative binding in contrast to collagen. The latter molecule, with its larger molecular size and weight is specialized for a biologically structural function and has a higher proportion of possible reactive sites which appear relatively more accessible to ligands. A study of the interactions between corticosteroids and collagen thus provides the opportunity to investigate a protein which is very different from the much studied serum albumin. Because of the limited information available regarding the interaction of steroid drugs and collagen at the molecular level, studies of this nature are relevant to the understanding of the mode of action of steroid compounds which are such an important group of therapeutic substances used in modern medicine.
- Full Text:
- Date Issued: 1975
- Authors: Kanfer, Isadore
- Date: 1975
- Subjects: Anti-inflammatory agents Collagen
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3849 , http://hdl.handle.net/10962/d1012620
- Description: Much research has been done on the formation of fibrils from solutions of soluble collagen in vitro in order to gain some knowledge of the mechanisms which may occur in vivo. The in vitro formation of fibres from solutions of collagen has been shown to be extremely sensitive to the nature of the solution environment and the presence of added chemical compounds, and thus constitutes an interesting system for the study of collagen-small molecule inter actions. The present study is concerned with the effects of various corticosteroid drugs, used medicinally as anti-inflammatory agents, on collagen in solution. As these corticosteroids are administered to reduce inflammation in conditions such as rheumatoid arthritis and a host of other pathological conditions in which collagen is implicated, this work has been undertaken in order to establish and charac teri ze any binding mechanisms which may be involved. Furthermore, the corticosteroid drugs available commercially in pure form as the free base or as the water-soluble ester salts offer an interesting range of structural and stereochemical variants for the study of their reaction with a complex and biologically important protein molecule such as collagen. A great deal of research on drug- protein interactions (Goldstein, 1949; Meyer and Guttman, 1968a) and more specifically, steroid-protein interactions have been reported over the years (Daughaday, 1959; Sandberg et al., 1966; Villee and Engel, 1961; Westphal , 1971). Comprehensive reports, however, on steroid-collagen interactions in vitro are conspicuously absent from modern scientific literature although relatively superficial accounts have been published (Menczel and Maibach, 1972; Eik-Nes et al., 1954). Although work involving the above has appeared relating specifically to the effects of steroids on collagen biosynthesis both in vivo and in vitro there have been minimal accounts of steroid-collagen interactions tailored to characterize the binding at the molecular level. The effect of corticosteroids on the metabolism of connective tissue has also received special attention (Asboe-Hansen, 1959; Kivirikko, 1953; Nakagawa and Tsurufuji, 1972). Recently, Uitto et al. (1972) reported the effects of several anti-inflammatory corticosteroids on collagen biosynthesis in vitro, whilst Aalto and Kulonen (1972) reported the effects of several antirheumatic drugs on the synthesis of collagen and other proteins in vitro. The interactions between collagen and certain drugs has also been briefly reviewed (Chvapil, 1967). Much data also exists on the binding of a wide range of small molecules and ions with serum albumin (Steinhardt and Reynolds, 1969; Scatchard, 1949; Klotz, 1950). Serum albumin, being specialized for a very general transport function and apparently designed for the purpose of combining with a large range of small molecules, has a proportion of possible reactive sites 'buried' within the molecule itself because of its folded conformation. In addition, serum albumin shows a high degree of cooperative binding in contrast to collagen. The latter molecule, with its larger molecular size and weight is specialized for a biologically structural function and has a higher proportion of possible reactive sites which appear relatively more accessible to ligands. A study of the interactions between corticosteroids and collagen thus provides the opportunity to investigate a protein which is very different from the much studied serum albumin. Because of the limited information available regarding the interaction of steroid drugs and collagen at the molecular level, studies of this nature are relevant to the understanding of the mode of action of steroid compounds which are such an important group of therapeutic substances used in modern medicine.
- Full Text:
- Date Issued: 1975
Paradoks as poësie: 'n ondersoek na enkele aspekte van die poësie van Breyten Breytenbach
- Authors: Van der Merwe, Anna Maria
- Date: 1975
- Subjects: Breytenbach, Breyten -- Criticism and interpretation
- Language: Afrikaans
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3636 , http://hdl.handle.net/10962/d1013302
- Full Text:
- Date Issued: 1975
- Authors: Van der Merwe, Anna Maria
- Date: 1975
- Subjects: Breytenbach, Breyten -- Criticism and interpretation
- Language: Afrikaans
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3636 , http://hdl.handle.net/10962/d1013302
- Full Text:
- Date Issued: 1975
Studies on achromobacter iophagus and other collagenolytic hide bacteria
- Authors: Welton, Richard Leslie
- Date: 1975
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:21099 , http://hdl.handle.net/10962/6367
- Description: From Introduction: Collagenases are enzymes capable of specifically attacking the native collagen helix under non-denaturing conditions at physiological conditions of pH, temperature and salt concentration. They are active only on collagen or its breakdown products and are without effect on any other fibrous or globular protein. In the laboratory, collagenases are used in investigations of the biosynthesis of collagen and for structural and immunochemical studies of collagens and collagen-like proteins; also they are proving their worth as agents for facilitating tissue transplantation and for cell-dispersion in tissue cultures . Established clinical applications of collagenases include the treatment of burns and dermal lesions; in addition they are being evaluated as agents for the removal of undesirable tissues such as herniated intervertebral discs and the sloughs resulting from cryogenic or cauterizing procedures. Moreover, as human collagenases are implicated in various pathological disorders involving connective tissue degradation, the roles played by these collagenases are being investigated in the hope of finding ways to arrest, control or treat the diseases.
- Full Text:
- Date Issued: 1975
- Authors: Welton, Richard Leslie
- Date: 1975
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:21099 , http://hdl.handle.net/10962/6367
- Description: From Introduction: Collagenases are enzymes capable of specifically attacking the native collagen helix under non-denaturing conditions at physiological conditions of pH, temperature and salt concentration. They are active only on collagen or its breakdown products and are without effect on any other fibrous or globular protein. In the laboratory, collagenases are used in investigations of the biosynthesis of collagen and for structural and immunochemical studies of collagens and collagen-like proteins; also they are proving their worth as agents for facilitating tissue transplantation and for cell-dispersion in tissue cultures . Established clinical applications of collagenases include the treatment of burns and dermal lesions; in addition they are being evaluated as agents for the removal of undesirable tissues such as herniated intervertebral discs and the sloughs resulting from cryogenic or cauterizing procedures. Moreover, as human collagenases are implicated in various pathological disorders involving connective tissue degradation, the roles played by these collagenases are being investigated in the hope of finding ways to arrest, control or treat the diseases.
- Full Text:
- Date Issued: 1975
Studies on the biology and ecology of Amblyomma Herbraem Koch, 1844 and other tick species (Ixodidae) of the Eastern Cape
- Authors: Norval, R A I
- Date: 1975
- Subjects: Ticks -- South Africa -- Eastern Cape , Ixodidae -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5871 , http://hdl.handle.net/10962/d1012968
- Description: Tick borne diseases of livestock are among the most important factors which have retarded economic development in many parts of Africa. Modification of the environment due to the expansion of agriculture into previously undeveloped areas, together with the introduction of livestock, has disturbed the natural balance which existed between ticks and indigenous hosts. Many tick species have adapted to domestic stock, and in some instances have spread over large areas which were previously uninfested. A number of tick species which are parasitic on domestic stock, eg. Amblyomma hebraeum Koch, 1844, Boophilus decoloratus (Koch, 1844), Rhipicephalus evertsi Neumann, 1897 and Rhipicephalus appendiculatus Netunann, 1901, are vectors of pathogenic micro-organisms. Unlike indigenous hosts however, domestic stock have no innate immunity to tickborne diseases. Intro., p. 1.
- Full Text:
- Date Issued: 1975
- Authors: Norval, R A I
- Date: 1975
- Subjects: Ticks -- South Africa -- Eastern Cape , Ixodidae -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5871 , http://hdl.handle.net/10962/d1012968
- Description: Tick borne diseases of livestock are among the most important factors which have retarded economic development in many parts of Africa. Modification of the environment due to the expansion of agriculture into previously undeveloped areas, together with the introduction of livestock, has disturbed the natural balance which existed between ticks and indigenous hosts. Many tick species have adapted to domestic stock, and in some instances have spread over large areas which were previously uninfested. A number of tick species which are parasitic on domestic stock, eg. Amblyomma hebraeum Koch, 1844, Boophilus decoloratus (Koch, 1844), Rhipicephalus evertsi Neumann, 1897 and Rhipicephalus appendiculatus Netunann, 1901, are vectors of pathogenic micro-organisms. Unlike indigenous hosts however, domestic stock have no innate immunity to tickborne diseases. Intro., p. 1.
- Full Text:
- Date Issued: 1975
The African press in South Africa
- Authors: St Leger, Frederick York
- Date: 1975
- Subjects: Press -- South Africa -- History Ethnic press -- South Africa -- History
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3328 , http://hdl.handle.net/10962/d1003116
- Description: In a plural society, riven by deep conflict, as is South Africa, where however the Press retains a considerable measure of freedom to contribute to the political process it is the political role which is of the greatest significance rather than, for example, the reflection by the Press of social values or the internal organisation of the Press as a social system. Although it is, of course, hardly possible completely to ignore these other aspects in any institutional study.
- Full Text:
- Date Issued: 1975
- Authors: St Leger, Frederick York
- Date: 1975
- Subjects: Press -- South Africa -- History Ethnic press -- South Africa -- History
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3328 , http://hdl.handle.net/10962/d1003116
- Description: In a plural society, riven by deep conflict, as is South Africa, where however the Press retains a considerable measure of freedom to contribute to the political process it is the political role which is of the greatest significance rather than, for example, the reflection by the Press of social values or the internal organisation of the Press as a social system. Although it is, of course, hardly possible completely to ignore these other aspects in any institutional study.
- Full Text:
- Date Issued: 1975
The population dynamics of an indigenous Psyllid Acizzia Russellae (Homoptera: Psyllidae )with special reference to the influence of the host plant Acacia Karroo
- Authors: Webb, John Warren
- Date: 1975
- Subjects: Homoptera , Jumping plant-lice , Insect populations , Insects -- Ecology , Acacia -- Karroo
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5894 , http://hdl.handle.net/10962/d1013415
- Description: The biology and ecology of an indigenous, non-pest insect on an indigenous host plant were investigated. The study includes a taxonomic description of this new species, an account of its general biology, monitoring of the seasonal fluctuations in numbers of the psyllid and its hymenopterous parasitoids, and a study of various aspects of the host plant, including nitrogen levels, water stress, leaf hardness, and the effect of cutting, in relation to spatial and temporal differences in insect population numbers. Natural enemies, inter- and intra-specific competition had very little determining influence on the population numbers of the psyllid. Temperature and humidity had little direct effect, but may have influenced the population dynamics via its effects on the host plant. Seasonal patterns in psyllid numbers followed fluctuations in nitrogen levels; statistically valid correlations were obtained between leaf nitrogen and psyllid numbers on individual trees at various times. These findings were supported by the results of preliminary laboratory experiments employing different fertilizer treatments. No effect of water stress or leaf hardness was clearly discerned. Cutting of trees altered the characteristics of the subsequent regenerative growth so as to allow massive psyllid infestations to develop, thus showing the tremendous importance of the host plant in determining population levels in this insect. Preliminary investigations of the nature and mechanism of this effect were conducted, and its significance is discussed. The relevance of these findings to modern concepts of regulation in insect populations and to principles of pest management is discussed.
- Full Text:
- Date Issued: 1975
- Authors: Webb, John Warren
- Date: 1975
- Subjects: Homoptera , Jumping plant-lice , Insect populations , Insects -- Ecology , Acacia -- Karroo
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5894 , http://hdl.handle.net/10962/d1013415
- Description: The biology and ecology of an indigenous, non-pest insect on an indigenous host plant were investigated. The study includes a taxonomic description of this new species, an account of its general biology, monitoring of the seasonal fluctuations in numbers of the psyllid and its hymenopterous parasitoids, and a study of various aspects of the host plant, including nitrogen levels, water stress, leaf hardness, and the effect of cutting, in relation to spatial and temporal differences in insect population numbers. Natural enemies, inter- and intra-specific competition had very little determining influence on the population numbers of the psyllid. Temperature and humidity had little direct effect, but may have influenced the population dynamics via its effects on the host plant. Seasonal patterns in psyllid numbers followed fluctuations in nitrogen levels; statistically valid correlations were obtained between leaf nitrogen and psyllid numbers on individual trees at various times. These findings were supported by the results of preliminary laboratory experiments employing different fertilizer treatments. No effect of water stress or leaf hardness was clearly discerned. Cutting of trees altered the characteristics of the subsequent regenerative growth so as to allow massive psyllid infestations to develop, thus showing the tremendous importance of the host plant in determining population levels in this insect. Preliminary investigations of the nature and mechanism of this effect were conducted, and its significance is discussed. The relevance of these findings to modern concepts of regulation in insect populations and to principles of pest management is discussed.
- Full Text:
- Date Issued: 1975
"Complexes of carbohydrates with magnesium-ions" : "the isolation of an oligosaccharide containing L-galactose from the polysaccharide of Aeodes orbitosa" : "horizontal cellulose colum chromatography of sugars"
- Authors: Van der Linde, Michael John
- Date: 1974
- Subjects: Magenesium , Ions , Carbohydrates , Oligosaccharides , Polysaccharides , Chromatographic analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4504 , http://hdl.handle.net/10962/d1013282
- Description: Sugar complexes with anions and cations are reviewed. Evidence is presented for the formation of a complex of stoichiometry 1:1 in aqueous solution between methyl-α-Dglucopyranoside and magnesium perchlorate. The complex may be detected by the method of continuous variations. Measurements of changes in the proton chemical shifts of the glucoside in dauterium oxide, indicate that the complex is probably formed between the vicinal hydroxyl groups at C-2 and C-3 of the pyranoside ring and the hydrated cation. At elevation temperatures there is evidence for the presence of a complex of stoichiometry 2:1. Experiments conducted on cellulose indicate the possible formation of cellulose - magnesium-ion complexes . These complexes provide an explanation for the "protective action" of mEgnesium compounds on the cellulose portion of pulp during alkali-oxygen bleaching.
- Full Text:
- Date Issued: 1974
- Authors: Van der Linde, Michael John
- Date: 1974
- Subjects: Magenesium , Ions , Carbohydrates , Oligosaccharides , Polysaccharides , Chromatographic analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4504 , http://hdl.handle.net/10962/d1013282
- Description: Sugar complexes with anions and cations are reviewed. Evidence is presented for the formation of a complex of stoichiometry 1:1 in aqueous solution between methyl-α-Dglucopyranoside and magnesium perchlorate. The complex may be detected by the method of continuous variations. Measurements of changes in the proton chemical shifts of the glucoside in dauterium oxide, indicate that the complex is probably formed between the vicinal hydroxyl groups at C-2 and C-3 of the pyranoside ring and the hydrated cation. At elevation temperatures there is evidence for the presence of a complex of stoichiometry 2:1. Experiments conducted on cellulose indicate the possible formation of cellulose - magnesium-ion complexes . These complexes provide an explanation for the "protective action" of mEgnesium compounds on the cellulose portion of pulp during alkali-oxygen bleaching.
- Full Text:
- Date Issued: 1974
A contribution to the biology of Pseudodiaptomus hessei (Mrázek) (Copepoda : Calanoida) in Lake Sibaya, South Africa
- Authors: Hart, Robert Clynton
- Date: 1974
- Subjects: Copepoda -- South Africa Freshwater biology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5854 , http://hdl.handle.net/10962/d1012210
- Description: Aspects of the biology of the calanoid copepod Pseudodiaptomus hessei are described, with particular reference to its vertical migratory behaviour. The present investigations were carried out largely by means of Nansen-type plankton nets, but several new pieces of apparatus were developed and. are described herein. The daytime vertical distribution of P. hessei varies according to developmental stage and depth of water. In shallow areas of the lake the entire population is benthic or in very close association with the lake bed. In the deepest part of the lake (40m) the naupliar and early (i.e. C I - C III) copepodite stages are essentially pelagic, but the adult and late (i.e. C IV - C V) copepodite stages are predominantly benthic and may be quiescent or infaunal. During the hours of darkness, the calanoids are distributed through the water column. The nauplii are consistently abundant in the surface waters but the distribution of the other stages is not regular. The distribution can be related to lunar intensity in many cases, with the post-naupliar stages frequently occurring deeper in the water column on bright moonlight nights and in the surface waters on overcast moolnless nights. The dusk ascent and dawn descent of the calenoids is clearly related to changes in light penetration in most individuals. The movements of a fraction of the adults occur in the apparent absence of adequate light cues. This behaviour is shown more extensively by the adult females. An endogenous activity rhythm has been shown in the species under laboratory conditions and it is suggested that this may play a part in the migratory movements. A basic examination of the feeding methods, feeding appendages and food sources of adult and late copepodite stages has been made. Changes in feeding intensity through twenty-four have been examined in the field and under laboratory conditions. Using as an index of feeding intensity, the proportion of animals with food in their guts, it has been shown that a pronounced diel difference occurs in adult calanoids in the lake. Feeding is almost entirely restricted to the nocturnal presence of the calanoids in the water coloumn. This difference exists in the pre-adult copepodite stages, but is not nearly as striking. The absence of feeding during daylight is not readily accounted for in terms of food availability and it is attributed to the quiescent or possible infaunal existence of the adults. A periodicity in egg hatching has been shown Naupliar release from the parental egg sac is predominantly a nocturnal phenomenon in the hot and cool seasons. It is suggested that this may be important in attaining a favourable vertical distritution for the nauplii, and may be important in the distribution of the species. A preliminary acccunt of the seasonal cycle and population dynamics of P. hessei is given, based on data collected over two years at a single station. Seasonal changes in calanoid abundance are intermediate between those recorded in truly tropical areas and in temperate latitudes. Potential food sources show relatively little change. The vertical migration of P. hessei is considered in relation to its apparent ecological significance and comparisons are drawn with observations made on the same species or other pseudodiaptomids in estuaries and lagoons elsewhere.
- Full Text:
- Date Issued: 1974
- Authors: Hart, Robert Clynton
- Date: 1974
- Subjects: Copepoda -- South Africa Freshwater biology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5854 , http://hdl.handle.net/10962/d1012210
- Description: Aspects of the biology of the calanoid copepod Pseudodiaptomus hessei are described, with particular reference to its vertical migratory behaviour. The present investigations were carried out largely by means of Nansen-type plankton nets, but several new pieces of apparatus were developed and. are described herein. The daytime vertical distribution of P. hessei varies according to developmental stage and depth of water. In shallow areas of the lake the entire population is benthic or in very close association with the lake bed. In the deepest part of the lake (40m) the naupliar and early (i.e. C I - C III) copepodite stages are essentially pelagic, but the adult and late (i.e. C IV - C V) copepodite stages are predominantly benthic and may be quiescent or infaunal. During the hours of darkness, the calanoids are distributed through the water column. The nauplii are consistently abundant in the surface waters but the distribution of the other stages is not regular. The distribution can be related to lunar intensity in many cases, with the post-naupliar stages frequently occurring deeper in the water column on bright moonlight nights and in the surface waters on overcast moolnless nights. The dusk ascent and dawn descent of the calenoids is clearly related to changes in light penetration in most individuals. The movements of a fraction of the adults occur in the apparent absence of adequate light cues. This behaviour is shown more extensively by the adult females. An endogenous activity rhythm has been shown in the species under laboratory conditions and it is suggested that this may play a part in the migratory movements. A basic examination of the feeding methods, feeding appendages and food sources of adult and late copepodite stages has been made. Changes in feeding intensity through twenty-four have been examined in the field and under laboratory conditions. Using as an index of feeding intensity, the proportion of animals with food in their guts, it has been shown that a pronounced diel difference occurs in adult calanoids in the lake. Feeding is almost entirely restricted to the nocturnal presence of the calanoids in the water coloumn. This difference exists in the pre-adult copepodite stages, but is not nearly as striking. The absence of feeding during daylight is not readily accounted for in terms of food availability and it is attributed to the quiescent or possible infaunal existence of the adults. A periodicity in egg hatching has been shown Naupliar release from the parental egg sac is predominantly a nocturnal phenomenon in the hot and cool seasons. It is suggested that this may be important in attaining a favourable vertical distritution for the nauplii, and may be important in the distribution of the species. A preliminary acccunt of the seasonal cycle and population dynamics of P. hessei is given, based on data collected over two years at a single station. Seasonal changes in calanoid abundance are intermediate between those recorded in truly tropical areas and in temperate latitudes. Potential food sources show relatively little change. The vertical migration of P. hessei is considered in relation to its apparent ecological significance and comparisons are drawn with observations made on the same species or other pseudodiaptomids in estuaries and lagoons elsewhere.
- Full Text:
- Date Issued: 1974