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Hop as an anti-cancer drug target

  • Authors: Vaaltyn, Michaelone Chantelle
  • Date: 2020
  • Subjects: Uncatalogued
  • Language: English
  • Type: thesis , text , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/164704 , vital:41156 , doi:10.21504/10962/164704
  • Description: Thesis (PhD)--Rhodes University, Biochemistry and Microbiology, 2020
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  • Date Issued: 2020

How to do things with speeches: a critical discourse analysis of military coup texts in Nigeria

  • Authors: Bello, Umar
  • Date: 2020
  • Subjects: Speeches, addresses, etc., Nigerian -- 20th century -- History and criticism , Critical discourse analysis -- Nigeria , Corpora (Linguistics) , Social sciences -- Philosophy , Intertextuality , Interpellation -- Nigeria , Forensics (Public speaking) , Oratory -- Nigeria , Nigeria -- Politics and government -- 1960- , Nigeria -- Social conditions -- 1960-
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/143336 , vital:38234
  • Description: Coup speeches that usher the military into political power in Nigeria are the central focus of this thesis. There are seven coup speeches that are notable in the changing of the political course in Nigeria and in enabling the military to rule Nigeria for 30 years, establishing another alternative political construct and party (Bangura 1991). The seven coup speeches along with two others, one a colonial proclamation of conquest and the other a counter coup speech (altogether making nine) constitute the data of this thesis. The analysis done here uses Critical Discourse Analysis, based on a combination of Fairclough (1989, 2001), Fairclough and Fairclough (2012), Thompson’s (1984, 1988 and 1990) works with complementary insights by Chilton (2004), to analyze the speeches in order to understand the ideologies, perceptions and arguments of the coup makers enshrined in the texts. I also employ a concordance analytic system in corpus linguistics to sort uses of important terms and lexical items. The analysis is divided into three broad parts, namely: an analysis of representation of social actors and their action, an analysis of the processes of interpellation and then an analysis of the premises of the arguments contained in the speeches. In the concluding part, there is a discussion of the dialectical nature of the coup speeches especially in the areas of mutual influences which aids in the gradual sedimentation of the political ideology of the military. In particular, there is a longitudinal intertextual analysis across all the speeches, from the earliest to the latest, to see how a coup speech genre is created. The contribution of this work to knowledge is in terms of combining discourse analysis and social theory to illuminate some aspects of Nigeria’s socio- political crises in depth and multifariously. This work helps in understanding the nature of Nigerian autocratic democracy, subservient followership by the citizenry and the supremacy of the military elite. The work employs a novel combination of representation, argumentation, interpellation and constitutive intertextuality in understanding military discourse. It looks at speaker intention, the exploitation of interpretation or reception and the formation of subjects in general and each with its importance and social context. The work as a whole reveals that the military try to build legitimacy by way of establishing authority through rhetorical arguments in varying degrees. These arguments are laid bare, and what they discern is that charges are decidedly trumped up by the military against their opponents and constructed to suit the spin of their moments. The coup makers in some instances construct strawmen of opponents and then go ahead to attack their constructed assumptions or they charge without substance using nominalizations, metaphorical constructions and presuppositions. They apply stipulative definitions and emotionally loaded words in evaluating their actions favourably and also in the negative evaluation of the actions of the opponents. At the level of interpellational analysis, the data reveals the use of language in gradually hailing the citizens as military subjects. The role of the audience changes here i.e. from those to be convinced in rhetorical evaluation of opponents to those to be firmly controlled. The persistent hailing and positioning of the citizens as military subjects help in concretizing their subjecthood. The reaction of the people in affirmation of support to the rule of the military is crucial and it completes the interpellation process. As observed by Clark (2007, 141) “many African societies are so inured to military intervention as not to regard it as aberrant”. This inuring of the societies has to do with hegemonic ideological practices in military discourses claiming legitimacy and the right to rule. At the reception level, this shows that most of the citizens have bought into the dominant ideology and are as such interpellated by it or have adopted what Hall (2015, 125) would call the ‘dominant-hegemonic position’. Aspects of argumentation, speech acts, and deontic modals used by the coup makers help in gradually solidifying the subservient nature of the citizens to the military junta. The diachronic and intertextual nature of the analysis also reveals that the colonial proclamation of conquest in Nigeria by Lord Fredrick Lugard possibly influenced the first coup speech in 1966 in terms of structure and genre. There are traces of the colonial proclamations found in the 1966 coup speech. In substance, the military appear to copy their colonial progenitors. Historically, the military were formed as an army of colonial conquest. There is a dialectical interplay between colonial discourse and military coup speeches. The first coup speech, for its part, influences other coup speeches and they in general impact on civilian political language. The work analyzes from the minute to the global and in this bid unties the layers of assumptions, constructions and points of views that underpin an otherwise objective presentation of reality. The study also engages social theory in illuminating aspects of discourse, social practice and political action. The works of post-structuralists like Foucault, Althusser, Bourdieu, Habermas, Laclau and Mouffe, Derrida etc. are employed in shedding light on the processes of social formation in the interpellation of subjects and in the construction of a new political authority by the military regimes.
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  • Date Issued: 2020

Informal learning in local farming practices by rural women in the Lake Chilwa Basin, Malawi: towards coping and adaptation to climate variability and climate change

  • Authors: Mphepo, Gibson Yadunda
  • Date: 2020
  • Subjects: Women -- Non-formal education - Malawi , Non-formal education -- Malawi , Women -- Malawi -- Social conditions , Crops and climate -- Malawi , Agricultural extension work -- Government policy -- Malawi , Environmental education -- Malawi
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/167540 , vital:41490
  • Description: Evidence reveals that informal learning is a neglected research area, globally and nationally. Informal learning, like formal and non-formal learning, is context specific. In the case of my study, the context was in local maize cultivation and the associated local farming practices which are also neglected. Research has shown that rural women in Malawi are significant change agents in socio-economic sectors, yet they are heavily affected by inequality. For example, extreme weather events of droughts and floods in the Lake Chilwa Basin, disproportionately affect more women than men because of their traditional gendered roles such as home care. The complexity of the dualistic nature of being change agents and victims of injustices at the same time offers a catalytic opportunity for potentially transformative social learning for transformative adaptation. Against this backdrop, I conducted a study to investigate and expand informal learning processes to contribute to building the resilience of women and other community members in Domasi and Nsanama Extension Planning Areas (EPA) within the Lake Chilwa Basin. Specifically, the study answered the following question: “How do drought and inter-seasonal dry spells influence informal learning processes to enable transformation adaptation among rural women cultivating maize in the Lake Chilwa Basin, Malawi?” To address the question, the first stage was to review local farming practices and the associated informal learning processes in Malawi. I then used third generation Cultural Historical Activity Theory (CHAT) as an overarching theoretical framework to guide the subsequent research processes which were split into three main phases: mirror data collection for expansive learning, formative change laboratory workshops, and data analysis and reflection. CHAT is a theoretical framework that helps us comprehend and analyze the relationship between the human mind (i.e. what people think and feel) and activity (what people do). It is a formative and activist learning theory that posits learning as occurring through collective activities to meet or change a common object (Mukute and Lotz-Sisitka, 2012, p. 345). For Koszalka and Wu, 2001(p. 493), within a CHAT framework, knowledge is socially constructed by individual learners, building on existing historical experiences, within the learners’ context. To construct this knowledge, learners use technology or mediating tools, as Vygotsky (1978) calls them. To collect mirror data, I conducted focus group discussions, observation studies, and document analysis. I also conducted key informant interviews with selected extension workers responsible for the two case study sites. The hub of my research constituted change laboratory workshops to expand learning through four of the seven expansive learning actions, namely questioning, analysis, modeling and testing the model. One of the essential procedures I relied on to expand learning during these change laboratory workshops was identification and analysis of contradictions that were mirrored back to women. The use of contradictions as fertile ground for learning is premised on Engstrom’s arguments that contradictions form a catalyst for learning. Data were analysed using two approaches: layered and power relations. A layered analysis is a step-by-step process of understanding a situation from the lower to a higher level (mature stage). For my research, this meant understanding sequential learning from questioning (session 1 – lower level) to testing the model (session 8 – higher level). The second data analysis approach, power relations, relates to the Women Empowerment in Agriculture Index (WEAI), a measure of the degree of women empowerment, their agency and inclusion in farming (Ruth et al., 2013, p. 3). I used this type of power analysis tool because my research was agriculture based. Both data analysis approaches relied on N-vivo which is a form of computer-based qualitative data management software. The software was ideal for my study which was also mostly qualitative. During phase 1 of data collection, I identified five local farming practices associated with local maize cultivation, a focus of my study. These practices were slash and burn (mphanje); traditional insect pest control measures; soil fertility enhancement techniques through kuojeka (crop residue incorporation) and livestock manure; traditional weather forecasts; and multiple cropping (mixed and sequential cropping). Among these, the most preferred by the women I interacted with were kuojeka, livestock manure and mixed cropping. I discovered that these local farming practices are informally learned mainly through word of mouth, observation, trials, women-dominated social networks and drama. I also discovered that some of these informal learning pathways are catalyzed by drought and dry spells. For example, during the 1949 and 2002 drought periods, women reported that they had learned new types of coping strategies such as the use of sawdust and banana root flour in place of maize flour to prepare nsima, a staple food in Malawi. During phase 2, change laboratory workshops, I identified 19 contradictions associated with local farming practices, most of which were related to the Government of Malawi bias towards modern farming practices such as hybrids. Other contradictions were related to traditional structures and norms and religion and traditional beliefs. Solutions were suggested for each of the contradictions. Some of these solutions were tested for their workability. These included setting up diversity blocks (demonstration plots) for local maize cultivation under irrigation and engagement of the youth through WhatsApp groups for the first time at the study sites. The results of the tests show that there is potential to transform local farming practices at the study sites and build social resilience against drought and dry spells. For example, from a local maize demonstration plot in Nsanama Extension Planning Area (EPA), farmers learned that kafula local maize is fast maturing and therefore cushions them against hunger as they wait for the main harvest in later months. Eighty-eight households shared local maize seed harvested from the demonstration gardens for upscaling. The Head of Nsanama EPA had also set up another demonstration garden in 2018-2019 growing season consisting of kafula at Nsanama EPA Headquarters for further informal learning purposes. This research has contributed new knowledge to the existing knowledge base about local farming practices and informal learning. These contributions are in the form of methods I used as well as results obtained. Among the key highlights of my contribution to the knowledge base is the development of scenarios as double stimulation tools for the emerging local farming activity system which emanates from the new model solutions resulting from change laboratory workshops. To the best of my knowledge, this was the first time rural communities were engaged in scenario development in Malawi. The first scenarios of this type were developed in 2010 for the Malawi State of Environment and Outlook Report and the process involved middle to senior managers of various institutions in Malawi. Through historical analysis, my research identified local crops that existed in the past but which are currently non-existent or rare. My study also identified unique local farming practices that even puzzled professionals, including the use of ripe banana peels of makumbuka and sukari to eradicate nansongole grass and native bamboos respectively. Both plant species are considered a nuisance in that they colonize land for cultivation. A breakthrough for radical transformation of local farming practices via informal learning requires development and review of relevant policies in Malawi. Such a process requires evidence. This research has provided background information for this process. For those policies already developed, this research has provided information that can help guide implementation of the generalized list of activities outlined in implementation plans of the respective policies.
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  • Date Issued: 2020

Invasion status and impact potential of Florida bass Micropterus floridanus in South Africa

  • Authors: Khosa, Dumisani
  • Date: 2020
  • Subjects: Micropterus floridanus , Florida largemouth bass -- South Africa , Black bass -- South Africa , Micropterus floridanus -- South Africa -- Habitat , Florida largemouth bass -- South Africa -- Habitat , Black bass -- South Africa -- Habitat , Introduced fishes -- Environmental aspects -- South Africa
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/165733 , vital:41276
  • Description: In South Africa, the introduction of alien fishes was largely driven by the development of recreational fishing opportunities. Government-backed programmes resulted in the introduction of four Black Bass species: Micropterus salmoides, Micropterus dolomieu, Micropterus punctulatus and Micropterus floridanus. These species are regarded as the primary threat to native biota and there is an urgent need to assess their spread and impacts. This thesis focuses on: assessing the current distribution of all four Black Basses; using genetic techniques to establish the extent of hybridisation between M. salmoides and M. floridanus, and using functional responses to assess the impact potential of M. salmoides and M. floridanus under the context dependence of temperatures and habitat complexity. Maximum entropy modelling of habitat suitability for Black Bass highlighted that M. salmoides had broad climatic suitability across South Africa, while suitability for M. dolomieu and M. punctulatus was restricted to the Cape Fold Ecoregion. An extensive area-based invasion debt was observed for all Micropterus spp. To better understand the dynamics of hybridisation between M. salmoides and M. floridanus, 38 species-diagnostic single nucleotide polymorphism was used to screen Black Bass populations from the Breede River and Kowie River catchments. Micropterus salmoides alleles were more prevalent than M. floridanus in both the Breede River catchments (69.1% and 30.9%, respectively) and Kowie River catchments (63.3% and 36.7%, respectively). However, isolated populations found in dams (reservoirs) remained free from hybridisation. To compare resource utilisation (i.e. per capita effects) between M. salmoides and M. floridanus, two experimental chapters were designed. Chapter 4 focuses on how temperature mitigates per capita effects between the two Black Basses. There was a significant decrease in per capita effects for M. salmoides with increasing temperatures, while the converse was true for M. floridanus. Chapter 5 gives an account of the influence of habitat complexity on per capita effects. The results showed that habitat complexity did mitigate per capita effects, specifically on M. floridanus, which showed a significant drop in per capita effects; however, no significant difference was recorded for M. salmoides. In the two experimental chapters, M. floridanus exhibited a superior per capita effect compared to M. salmoides, implying higher predation impact on native biota.
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  • Date Issued: 2020

Investigations into biological control options for Lycium ferocissimum Miers, African Boxthorn (Solanaceae) for Australia

  • Authors: Mauda, Evans Vusani
  • Date: 2020
  • Subjects: Lycium ferocissimum , Solanaceae -- Biological control -- Australia , Weeds -- Control -- Australia , Invasive plants -- Biological control -- Australia , Insects as biological pest control agents -- Australia , Insect-plant relationships
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/167142 , vital:41441
  • Description: Lycium ferocissimum Miers (Solanaceae) (African boxthorn or boxthorn) is a shrub native to South Africa,and has become naturalised and invasive in Australia and New Zealand. The plant is listed on the Noxious Weed List for Australian States and territories. Although other control methods are available, biological control presents a potentially sustainable intervention for reducing populations of this weed in Australia. In South Africa, the plant has been recorded from two allopatric populations, one in the Eastern Cape Province, the other in the Western Cape Provinces, however, there taxonomic and morphological uncertainties are reported in the literature. Therefore, before native range surveys for potential biological control agents could be considered, the taxonomic uncertainty needed to be resolved. The two geographically distinct areas, as well as the Australia population were sampled to assess morphological and genetic variation. All samples collected in Australia were confirmed as L.ferocissimum, with no evidence of hybridisation with any other Lycium species. Nuclear and chloroplast genetic diversity within L.ferocissimum across South Africa was high, and Australia was low, with no evidence of genetic seperation. One ehaplotypes found across Australia was found at only two sites in South Africa, both in the Western Cape, suggesting that the Australian lineage may have originated from this region. Ten samples from South Africa, putatively identified in the field as L.ferocissimum, were genetically characterised as different (unidentified) Lycium species. The majority of plants sampled were confirmed as L.ferocissimum, sharing a common haplotype (haplotype 5) with sampled specimens from Australia. Morphological analyses across different Lycium species in South Africa did not identify any leaf or floral characteristics unique to L.ferocissimum, and thus morphological identification in the native range remains problematic. Surveys for phytophagous in sects on L.ferocissimum were carried out regularly over a two-year period in the two regions. The number of insect species found in the Eastern Cape Province (55) was higher than in the Western Cape Province (41), but insect diversity based on Shannon indices was highest in the Western Cape Province. Indicator species analysis revealed eight insect herbivore species driving the differences in the herbivore communities between the two provinces. Based on insect distribution, abundance, feeding preference and available literature, three species were prioritised as potential biological control agents. These include the leaf-chewing beetles, Cassida distinguenda Spaeth (Chrysomelidae) and Cleta eckloni Mulsant (Coccinellidae), and the leaf-mining weevil, Neoplatygaster serietuberculata Gyllenhal (Curculionidae). Native range studies such as this are perhaps the most technically difficult and logistically time-consuming part of the biological control programme. Yet, the entire outcome of a programme depends on the suite of potential agents feeding on the weed. The information gained during this stage significantly contributed to the prioritization of agents for further host-range testing and possible release. Here we showed how molecular and genetic characterisations of the target weed can be us ed to accurately define the identity and phylogeny of the target species. In addition, the study also highlighted the importance of considering plant morphology and how phenotypic plasticity may influence infield plant identifications while conducting native range surveys. By gaining further information during long-term and wide spread native range surveys we were not just able to provide a list of herbivorous insect fauna and fungi associated with the plant, but were able to prioritise the phytophagous species that held the most potential as biological control agents.
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  • Date Issued: 2020

Journeying home, exile and transnationalism in Noni Jabavu and Sisonke Msimang’s memoirs

  • Authors: Masola, Athambile
  • Date: 2020
  • Subjects: Home in literature , Exile (Punishment) in literature , Women authors, Black -- South Africa -- History and criticism , Jabavu, Noni -- Criticism and interpretation , Msimang, Sisonke -- Criticism and interpretation
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/166529 , vital:41376
  • Description: This thesis examines the memoirs of two South Africa female writers: Noni Jabavu and Sisonke Msimang. In analysing their memoirs, this thesis sets out to contribute to the scholarship which centres the voices of Black women writers. It explores how both writers interrogate the concepts of home, exile and transnationalism. My focus is on the ways in which the writers theorise questions of belonging, and the extent to which their writing provides the opportunity to give voice to the complex nature of belonging. I read the narratives from these two writers alongside each other, as Noni Jabavu is Sisonke Msimang’s literary ancestor. By looking at their works alongside each other, this thesis becomes an intergenerational conversation between the past and the present. Using Black feminist thought and migratory subjectivity, this thesis examines the ways in which these writers connect the personal and the political. Noni and Sisonke navigate the private spaces of home in conversation with the political experience of exile and the nation. In the second chapter, I explore home as ekhaya, isizwe and an imaginary homeland. Ekhaya is the isiXhosa word denoting the family home, where there is a network of family rather than a nuclear family. Home is also isizwe: the isiXhosa word which evokes the nation state, which takes on layered meanings in the context of colonialism and apartheid. Home can also be an imaginary homeland, which is an imagined version of home after a long absence such as exile. In the third chapter, I examine three forms of exile: internal exile, estrangement and political exile. Finally, in chapter four, I analyse transnationalism: a border-crossing which raises questions of power, mobility and kinship. Looking at home, exile and transnationalism together allows for an exploration which challenges taken-for-granted ideas about place and identity. These memoirs illuminate the ways in which Black women constitute and reconstitute their identities according to the places which they access through travel.
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  • Date Issued: 2020

Long Waves of Strikes in South Africa: 1886–2019

Mechanism of action of non-synonymous single nucleotide variations associated with α-carbonic anhydrases II, IV and VIII

  • Authors: Sanyanga, T. Allan
  • Date: 2020
  • Subjects: Carbonic anhydrase , Carbonic anhydrase -- Therapeutic use , Nucleotides
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/167346 , vital:41470
  • Description: The carbonic anhydrase (CA) group of enzymes are Zinc (Zn2+) metalloproteins responsible for the reversible hydration of CO2 to bicarbonate (BCT or HCO− 3 ) and protons (H+) for the facilitation of acid-base balance and homeostasis within the body. Across all organisms, a minimum of six CA families exist, including, α (alpha), β (beta), γ (gamma), δ (delta), η (eta) and ζ (zeta). Some organisms can have more than one family, with exception to humans that contain the α family solely. The α-CA family comprises of 16 isoforms (CA-I to CA-XV) including the CA-VIII, CA-X and CA-XI acatalytic isoforms. Of the catalytic isoforms, CA-II and CA-IV possess one of the fastest rates of reaction, and any disturbances to the function of these enzymes results in CA deficiencies and undesirable phenotypes. CA-II deficiencies result in osteopetrosis with renal tubular acidosis and cerebral calcification, whereas CA-IV deficiencies result in retinitis pigmentosa 17 (RP17). Phenotypic effects generally manifest as a result of poor protein folding and function due to the presence of non-synonymous single nucleotide variations (nsSNVs). Even within the acatalytic isoforms such as CA-VIII that llosterically regulates the affinity of inositol triphosphate (IP3) for the IP3 receptor type 1 (ITPR1) and regulates calcium (Ca2+) signalling, the presence of SNVs also causes phenotypes cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CAMRQ3). Currently the majority of research into the CAs is focused on the inhibition of these proteins to achieve therapeutic effects in patients via the control of HCO− production or reabsorption as observed in glaucoma and diuretic medications. Little research has therefore been devoted into the identification of stabilising or activating compound that could rescue protein function in the case of deficiencies. The main aim of this research was to identify and characterise the effects of nsSNVs on the structure and function of CA-II, CA-IV and CA-VIII to set a foundation for rare disease studies into the CA group of proteins. Combined bioinformatics approaches divided into four main objectives were implemented. These included variant identification, sequence analysis and protein characterisation, force field (FF) parameter generation, molecular dynamics (MD) simulation and dynamic residue network analysis (DRN). Six variants for each of the CA-II, CA-IV and CA-VIII proteins with pathogenic annotations were identified from the HUMA and Ensembl databases. These included the pathogenic variants K18E, K18Q, H107Y, P236H, P236R and N252D for CA-II. CA-IV included the pathogenic R69H, R219C and R219S, and benign N86K, N177K and V234I variants. CA-VIII included pathogenic S100A, S100P, G162R and R237Q, and benign S100L and E109D variants. CA-II has been more extensively studied than CA-IV and CA-VIII, therefore residues essential to its function and stability are known. To discover important residues and regions within the CA-IV and CA-VIII proteins sequence and motif analysis was performed across the α-CA family, using CA-II as a reference. Sequence analysis identified multiple conserved residues between the two acatalytic CA-II and CA-IV, and the acatalytic CA-VIII isoforms that were proposed to be essential for protein stability. With exception to the benign N86K CA-IV variant, none of the other pathogenic or benign CA-II, CA-IV and CA-VIII SNVs were located at functionally or structurally important residues. Motif analysis identified 11 conserved and important motifs within the α-CA family. Several of the identified variants were located on these motifs including K18E, K18Q, H107Y and N252D (CA-II); N86K, R219C, R219S and V234I (CA-IV); and E109D, G162R and R237Q (CA-VIII). As there were no x-ray crystal structures of the variant proteins, homology modelling was performed to calculate the protein structures for characterisation. In CA-VIII, the substitution of Ser for Pro at position 100 (variant S100P) resulted in destruction of the β-sheet that the SNV was located on. Little is known about the mechanism of interaction between CA-VIII and ITPR1, and residues involved. SiteMap and CPORT were used to identify binding site amino for CA-VIII and results identified 38 potential residues. Traditional FFs are incapable of performing MD simulations of metalloproteins. The AMBER ff14SB FF was extended and Zn2+ FF parameters calculated to add support for metalloprotein MD simulations. In the protein, Zn2+ was noted to have a charge less than +1. Variant effects on protein structure were then investigated using MD simulations. Root mean square deviation (RMSD) and radius of gyration (Rg) results indicated subtle SNV effects to the variant global structure in CA-II and CA-IV. However, with regards to CA-VIII RMSD analysis highlighted that variant presence was associated with increases to the structural rigidity of the protein. Principal component analysis (PCA) in conjunction with free energy analysis was performed to observe variant effects on protein conformational sampling in 3D space. The binding of BCT to CA-II induced greater protein conformational sampling and was associated with higher free energy. In CA-IV and CA-VIII PCA analysis revealed key differences in the mechanism of action of pathogenic and benign SNVs. In CA-IV, wild-type (WT) and benign variant protein structures clustered into single low energy well hinting at the presence of more stable structures. Pathogenic variants were associated with higher free energy and proteins sampled more conformations without settling into a low energy well. PCA analysis of CA-VIII indicated the opposite to CA-IV. Pathogenic variants were clustered into low energy wells, while the WT and benign variants showed greater conformational sampling. Dynamic cross correlation (DCC) analysis was performed using the MD-TASK suite to determine variant effects on residue movement. CA-II WT protein revealed that BCT and CO2 were associated with anti-correlated and correlated residue movement, highlighting at opposite mechanisms. In CA-IV and CA-VIII variant presence resulted in a change to residue correlation compared to the WT proteins. DRN analysis was performed to investigate SNV effects of residue accessibility and communication. Results demonstrated that SNVs are associated with allosteric effects on the CA protein structures, and effects are located on the stability assisting residues of the aromatic clusters and the active site of the proteins. CA-II studies discovered that Glu117 is the most important residue for communication, and variant presence results in a decrease to the usage of the residue. This effect was greatest in the CA-II H107Y SNV, and suggests that variants could have an effect on Zn2+ dissociation from the active site. Decreases to the usage of Zn2+ coordinating residues were also noted. Where this occurred, compensatory increases to the usage of other primary and secondary coordination residues were observed, that could possibly assist with the maintenance of Zn2+ within the active site. The CA-IV variants R69H and R219C highlighted potentially similar pathogenic mechanisms, whereas N86K and N177K hinted at potentially similar benign mechanisms. Within CA-VIII, variant presence was associated with changes to the accessibility of the N-terminal binding site residues. The benign CA-VIII variants highlighted possible compensatory mechanisms, whereby as one group of N-terminal residues loses accessibility, there was an increase to the accessibility of other binding site residues to possibly balance the effect. Catalytically, the proton shuttle residue His64 in CA-II was found to occupy a novel conformation named the “faux in” that brought the imidazole group even closer to the Zn2+ compared to the “in” conformation. Overall, compared to traditional MD simulations the incorporation of DRN allowed more detailed investigations into the variant mechanisms of action. This highlights the importance of network analysis in the study of the effects of missense mutations on the structure and function of proteins. Investigations of diseases at the molecular level is essential in the identification of disease pathogenesis and assists with the development of specifically tailored and better treatment options especially in the cases of genetically associated rare diseases.
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  • Date Issued: 2020

Microbial fuel cells for remediation of metal rich wastewater coupled with bioelectricity generation

  • Authors: Mshoperi, Edith
  • Date: 2020
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/124112 , vital:35540
  • Description: Expected release date-April 2022
  • Full Text: false
  • Date Issued: 2020

Multiplexed Mass Spectrometry: Single, On-Bead, Detection Analysis Using MALDI-TOF MS

  • Authors: Twala, Busisiwe Victoria
  • Date: 2020
  • Subjects: Uncatalogued
  • Language: English
  • Type: thesis , text , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/164693 , vital:41155 , doi:10.21504/10962/164693
  • Description: Thesis (PhD)--Rhodes University, Biochemistry and Microbiology, 2020
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  • Date Issued: 2020

Negotiating family planning messages among Malawian men: A case study of vasectomy messages aimed at men in the Dedza and Karonga districts of Malawi

  • Authors: Ntaba, Jolly Maxwell
  • Date: 2020
  • Subjects: Social marketing -- Malawi , Vasectomy -- Social aspects -- Malawi , Malawians -- Attitudes , Men -- Malawi -- Attitudes , Men -- Conduct of life -- Malawi , Men -- Sexual behavior -- Malawi , Masculinity -- Malawi , Reproductive health in mass media -- Malawi , Reproductive health services -- Malawi
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/167200 , vital:41446
  • Description: Since the 1990s, vasectomy, the principal non-barrier method of contraception offered to men, has been vigorously promoted in Malawi, as a safe, effective and inexpensive form of birth control. Despite this marketing, the method has been chosen by only an estimated 0.01% of men in Malawi. This compares to about 11% of contraceptive using Malawian women, who have opted for tubal ligation, a more expensive and hazardous surgical procedure. Previous studies into this low uptake of vasectomy among men in Malawi, and other studies that have explored why no African country currently has a vasectomy rate of more than 1% of men, implicate cultural beliefs and traditional social practices among the key barriers to the diffusion of this particular innovation i.e. this method of contraception. Campaigns share new information in the hope that they will shift their audiences’ knowledge, and lead to changes in attitudes and practices, including the uptake of vasectomy. Social Marketing campaigns, even when they set out to explicitly accommodate these beliefs and challenge particular practices in order to promote various methods of contraception, are often inadequately conceived and sometimes poorly executed. Drawing on well-established theoretical perspectives developed by Cultural Studies scholars, including Reception Analysis and Thematic Analysis, this study investigates how men in two purposively selected districts of Karonga and Dedza in Malawi, interpret Social Marketing messages encouraging them to undergo vasectomies. The study examines key media texts and the nature of the involvement of a group of peer educators, dubbed ‘male champions’ in a 2017-2018 campaign by one of the leading Malawian providers of family planning services, the NGO Banja La Mtsogolo (BLM), to promote vasectomy. This is a purely qualitative case study that seeks to understand why these often-well-resourced campaigns have so little impact on the behaviour change they hope to inspire. Through individual interviews with the campaign’s designers, implementers, peer educators, focus group discussions with the campaign’s audiences, a close reading of texts used in the campaign, and observation, this study explores the circuits of communication and culture, through mechanisms of resonance, disconnection and even cognitive dissonance between the ‘encoders’ of the family planning messages and the decoders i.e. the intended audiences of the campaign. The study argues that as a result of several ‘modernist’ assumptions and outlooks, the campaign was unable to fully grasp the complex and contextually nuanced socio-cultural practices that factor into consideration of the campaign’s messages and the non-adoption of the proposed vasectomy method. The study further reveals, as many other studies have also observed, that the interpretation of the text promoting vasectomy is a complex process that is significantly shaped by the worldviews and lived experiences of the audiences. These views, as this study explores, are often complex and contradictory, interfacing aspirations of modernity with deeply held ‘traditional’ beliefs and practices. Although the campaigns are effective at transferring knowledge – it finds most targeted men have a relatively good understanding of the method and its efficacy – their prevailing socio-cultural attitudes and dispositions provide a strong countervailing discourse to the preferred reading of the campaign messages. This discourse exhorts having children, or the capacity to have children, even after a man has had several before, in current or previous marriages, or even in old age, as desirable and ‘rational’. The study therefore proposes, arising out of this detailed ethnographic research, a revised approach that argues that several social and cultural ‘vectors’ or ‘spheres of influence' need to be considered in new ways, in order to develop meaningful interventions in the promotion of vasectomy. This includes specific strategies to understand and challenge: 1. The enduring power of social stigma and scorn, and the notion of social shame. 2. The deep interplay between fertility and having children to notions of marriage, even in second or third marriages, and the interplay with perceptions of economic ‘value’ of children in the domestic political economy of marriage. 3. The embedded nature of provable fertility to notions of manhood. 4. The complex and nuanced involvement, at many levels, of broader social/family structures/personages in ‘personal’ decisions. 5. Forms of ‘hyperbolic discounting’, i.e. the calculating of precarious futures in various scenarios and its impact on current shorter-term calculations and gratifications. 6. Unusually high rates of infidelity in marriage, and seemingly low levels of trust in many partnerships. 7. The key role of interpersonal communication, i.e. the face-to-face elements of what are usually media-centric Social Marketing campaigns. The study recommends a more layered and nuanced approach to the promotion of vasectomy, propelled by a deeper understanding of these kinds of contexts and the interpretive power of the intended audience, as well as more nuanced segmentation of audiences, and more judicious use of peer-educators to support and deepen the mass media components of these communication campaigns.
  • Full Text:
  • Date Issued: 2020

NFComms: A synchronous communication framework for the CPU-NFP heterogeneous system

  • Authors: Pennefather, Sean
  • Date: 2020
  • Subjects: Network processors , Computer programming , Parallel processing (Electronic computers) , Netronome
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/144181 , vital:38318
  • Description: This work explores the viability of using a Network Flow Processor (NFP), developed by Netronome, as a coprocessor for the construction of a CPU-NFP heterogeneous platform in the domain of general processing. When considering heterogeneous platforms involving architectures like the NFP, the communication framework provided is typically represented as virtual network interfaces and is thus not suitable for generic communication. To enable a CPU-NFP heterogeneous platform for use in the domain of general computing, a suitable generic communication framework is required. A feasibility study for a suitable communication medium between the two candidate architectures showed that a generic framework that conforms to the mechanisms dictated by Communicating Sequential Processes is achievable. The resulting NFComms framework, which facilitates inter- and intra-architecture communication through the use of synchronous message passing, supports up to 16 unidirectional channels and includes queuing mechanisms for transparently supporting concurrent streams exceeding the channel count. The framework has a minimum latency of between 15.5 μs and 18 μs per synchronous transaction and can sustain a peak throughput of up to 30 Gbit/s. The framework also supports a runtime for interacting with the Go programming language, allowing user-space processes to subscribe channels to the framework for interacting with processes executing on the NFP. The viability of utilising a heterogeneous CPU-NFP system for use in the domain of general and network computing was explored by introducing a set of problems or applications spanning general computing, and network processing. These were implemented on the heterogeneous architecture and benchmarked against equivalent CPU-only and CPU/GPU solutions. The results recorded were used to form an opinion on the viability of using an NFP for general processing. It is the author’s opinion that, beyond very specific use cases, it appears that the NFP-400 is not currently a viable solution as a coprocessor in the field of general computing. This does not mean that the proposed framework or the concept of a heterogeneous CPU-NFP system should be discarded as such a system does have acceptable use in the fields of network and stream processing. Additionally, when comparing the recorded limitations to those seen during the early stages of general purpose GPU development, it is clear that general processing on the NFP is currently in a similar state.
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  • Date Issued: 2020

Non-marital fertility in South Africa: trends, determinants and implications

  • Authors: Kara, Reesha
  • Date: 2020
  • Subjects: Fertility, Human -- South Africa , Child rearing -- South Africa , Parenting -- South Africa , Motherhood -- South Africa
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/165620 , vital:41264
  • Description: Background: Non-martial fertility is traditionally associated with teenage pregnancy however international and South African literature has detailed increases in the number of adult women who are having children outside of a marriage. South African literature on non-marital fertility is sparse as it lacks a national overview of the trends and determinants of non-marital fertility among women aged 30 and older. The aim of this study was to present a national overview of non-marital fertility among women aged 30 and older. South African’s attitudes to and opinions of non-marital fertility were also investigated, and the lived realities of older mothers were explored. Methods: A mixed-methods research design was employed where the General Household Survey, National Income Dynamics Study and the South African Social Attitudes Survey were the main data sources. Using these data sets, descriptive and inferential statistics were computed using Stata. Using purposive and snowball sampling, four never-married older mothers (NMOMs) from KwaZulu-Natal (Durban) were identified as research participants. The in-depth life histories of these women were collected through face-to- face semi-structured interviews. Results: The results show an 18.43% increase in never-married mothers aged 15- 49 between 2002 and 2017 and interestingly, this increase is not necessarily driven by older mothers (30-49). NMOMs belonged to households with a lower average per capita total monthly household income (R1873.91) compared to all mothers aged 30-49 (R3428.76). NMOMs were also more likely to live in female-headed households (89.52%), to be household heads (64.22%) and to live in traditional areas (35.72%). Between 2002 and 2017, there was a 76.76% increase in mothers (aged 30-49) who were never married and a 7.74% decrease in those who were married, indicating a change in the marital profile of mothers. Despite this national increase in non-marital fertility, South African’s believe that premarital sexual activity is wrong, and that childbearing should take place within a marriage. Similar sentiments were echoed in the in-depth life histories as being the sole breadwinner and primary caregiver, the research participants experienced challenges as single mothers. Conclusion and recommendations: The study has found that there has been an increase in non-marital fertility in South Africa between 2002 and 2017 and that there is an economic element to non-marital fertility in the country. Additional research into non-marital fertility at a national level is recommended with a focus on all women aged 15-49.
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  • Date Issued: 2020

Not Yet Uhuru! Attuning to, re-imagining and regenerating transgressive decolonial pedagogical praxis across times Khapa(ring) the rising cultures of change drivers in contemporary South Africa

  • Authors: Kulundu-Bolus, Injairu
  • Date: 2020
  • Subjects: Not Yet Uhuru (Arts Project) , Anti-imperialist movements -- South Africa , Education -- Social aspects -- South Africa , Social change -- South Africa , Education -- Philosophy -- South Africa , Arts and society -- South Africa
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/166081 , vital:41327 , 10.21504/10962/166081
  • Description: The “Not yet Uhuru!” project positions itself as emancipatory African research in motion. It is a regenerative project that responds to the concern that whilst dominant discourses can articulate what African states, societies and economies are not, we still know very little about what they actually are. This is a particularly important gap in how research on Africa is conceptualised, especially as it pertains to apprehending the futures that the majority of young people on the continent are instinctively leading themselves to (Mbembe, 2001, p.9). The project seeks to forgo youth development strategies that act as a form of containment by prescribing normative aspects of citizenship on young leaders in ways that stifle the transgressive impulses they have reason to value (Kelley in Tuck and Yang, 2014, p.89). The study traces rising cultures in transgressive decolonial pedagogical praxis across times, as a way of “khapa(ring)” or accompanying the contemporary questions that Change Drivers in South Africa hold at the edge of their praxis. The study co-conspired with 21 Change Drivers in South Africa who were interested in regenerating and re-imagining what transgressive decolonial praxis could be in these times based on their experiences and learnings. Residential art-based workshops that explored each co-conspirator’s offerings on the subject were distilled through the medium of film. These in turn were analysed using an “ethics of attunement” that produced songs as a reflexive pedagogical tool (Lispari, 2014, p.176). Sharing the resonate echoes of their praxis through song created another iterative reflection on their praxis two years after their initial offerings. As a way of weaving together the findings with a historical perspective, the resonant praxis of Change Drivers was put into conversation with three unconventional reviews that trace impulses around transgressive decolonial pedagogical praxis through fictional texts, political theory, poetry and intergenerational analysis, in order to surface resonant themes in praxis that echo across different times in history. This methodology sought to engage the question of the archive in pluriversal ways that appealed to different sensibilities, including the imaginative and hermeneutical, the traditionally analytical as well as the gifts of the lyrical and the erotic as different conceptual threads needed to resource the study. The reviews additionally spanned periods in the history of the continent that hold questions around precolonial and nascent colonial encounters, efforts to transgress within the liberatory movements and the intergenerational transmissions embedded in women and queer people’s struggles. The themes that coalesced across times were leveraged into capsules of rising cultures that form an experimental nexus for the practice of transgressive decolonial pedagogical praxis that is already underway. These rising cultures were conceptualised as meditations on what it means to live into a vision of home built on the explorations of a paradigm of peace, humanness, pluriversality and decolonial love for those like and unlike us that strive for freedom on this continent (Dlala, 2017, p.52; Ndlovu- Gatsheni, 2013, p.142; Gqola, 2017, pp.197, 199). The rising ultures were reconciled through the creation of a litany that chronicles different refrains in transgressive decolonial pedagogical praxis in contemporary times. The litany is a tool that charts particular experiences that are surfacing as symptomatic. It seeks to generously surface the contradictions that we are collectively starting to see past, whilst acknowledging the tensions that we need to straddle, integrate and navigate towards greater synthesis. The litany is an honest way of acknowledging the glimpses gained of who we are in this present moment, while we continually challenge ourselves to open up to questions about what it means to grapple towards decolonial futures. This stance has influenced my role as an educator to unconditionally embrace movements that already underway, and reflect these back to those that I am conspiring with in ways that promote an ethic of care, solidarity and critical engagement. The study celebrates what is possible when we do not theorise ourselves away from the questions embedded in our current praxis. This is an ethic that chooses to stay close to the phenomena arriving at present, whilst acknowledging the historical experiences that echo it as a collective pulse for meaningful experimentation and praxis. The study believes by being faithful to ways of amplifying, integrating and reflecting what has been emerging for us over time, we build our capacity to better respond with an ethic centred on transgressive decolonial pedagogical praxis. This is the kind of accompaniment and care that Change Drivers across the continent deserve as they make the way towards a future worthy of their longing (Rushdie, 1999).
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  • Date Issued: 2020

Nutrient impacts on grasses and legumes growing in communal pasture soil in relation to mycorrhizal activity

  • Authors: Mkile, Zolani
  • Date: 2020
  • Subjects: Uncatalogued
  • Language: English
  • Type: thesis , text , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/164512 , vital:41125 , doi:10.21504/10962/164512
  • Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020
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  • Date Issued: 2020

Passphrase and keystroke dynamics authentication: security and usability

  • Authors: Bhana, Bhaveer
  • Date: 2020
  • Subjects: Computer security -- Management , Computers -- Access control -- Codewords , Computers -- Access control -- Keystroke timing authentication , Entropy (Information theory)
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/146663 , vital:38546
  • Description: It was found that employees spend a total 2.25 days within a 60 day period on password related activities. Another study found that over 85 days an average user will create 25 accounts with an average of 6.5 unique passwords. These numbers are expected to increase over time as more systems become available. In addition, the use of 6.5 unique passwords highlight that passwords are being reused which creates security concerns as multiple systems will be accessible by an unauthorised party if one of these passwords is leaked. Current user authentication solutions either increase security or usability. When security increases, usability decreases, or vice versa. To add to this, stringent security protocols encourage unsecure behaviours by the user such as writing the password down on a piece of paper to remember it. It was found that passphrases require less cognitive effort than passwords and because passphrases are stronger than passwords, they don’t need to be changed as frequently as passwords. This study aimed to assess a two-tier user authentication solution that increases security and usability. The proposed solution uses passphrases in conjunction with keystroke dynamics to address this research problem. The design science research approach was used to guide this study. The study’s theoretical foundation includes three theories. The Shannon entropy formula was used to calculate the strength of passwords, passphrases and keystroke dynamics. The chunking theory assisted in assessing password and passphrase memorisation issues and the keystroke-level model was used to assess password and passphrase typing issues. Two primary data collection methods were used to evaluate the findings and to ensure that gaps in the research were filled. A login assessment experiment collected data on user authentication and user-system interaction for passwords and passphrases. Plus, an expert review was conducted to verify findings and assess the research artefact in the form of a model. The model can be used to assist with the implementation of a two-tier user authentication solution which involves passphrases and keystroke dynamics. There are a number of components that need to be considered to realise the benefits of this solution and ensure successful implementation.
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  • Date Issued: 2020

Performance, functionalism and form in Ịzọn oral poety

  • Authors: Armstrong, Imomotimi
  • Date: 2020
  • Subjects: Ijo (African people , Ijo language , Folk poetry, Ijo , Folk poetry -- Nigeria
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/140641 , vital:37906
  • Description: Since the publication of Ruth Finnegan’s influential Oral Literature in Africa, way back in 1970, scholars have been paying earnest attention to oral traditions on the African continent. That seminal book pointed out to Africanist scholars the need to urgently collect and document the oral literatures of their various ethnic groups before they die out. However, it is the verbal arts of the major ethnic groups on the continent that very often benefit from this collection and documentation, as it were. Therefore, this study sought to examine the oral poetry of the Ịzọn, a minority ethnic nationality, located in the oil-rich Niger Delta region of Nigeria. The study employed unstructured interviews and participant observations to collect the data for the research. The transcribed and translated data was examined through three eclectic theories to the study of folklore: Russian formalism, performance and functionalism. The study found out that Ịzọn oral poetry is a combination of songs and one person’s praise chants. Moreover, it revealed that praise chanting is a recent practice amongst the Ịzọn that was introduced into Ịzọnland by Chief Adolphus Munamuna from the Yoruba of southwestern Nigeria. Furthermore, the study established that oral poetry plays important roles amongst the people. Besides, it ascertained that the performance of some sub-categories of the poetry is highly dramatic and theatrical. It also discovered that stylistic techniques such as formula, parallelism, proverb, ideophone, praise title, metaphor, repetition, alliteration, assonance, vowel lengthening, amongst others, give the poetry the quality of “literariness.” In addition, the study found out that the poetry, like oral poetry in other ethnic groups, demonstrates the three qualities of change, adaptability and survival. The study has contributed to existing scholarship on African oral traditions in the sense of collecting, documenting and generating awareness on Ịzọn oral poetry, most importantly pointing out the existence of praise chanting amongst a people who had no such culture and the conditions that gave rise to that practice.
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  • Date Issued: 2020

Post release evaluation of the distribution and efficacy of Eccritotarsus catarinensis and Eccritotarsus eichhorniae on Pontederia crassipes in South Africa

  • Authors: Maseko, Zolile
  • Date: 2020
  • Subjects: Water hyacinth -- Biological control -- South Africa , Weeds -- Biological control -- South Africa , Miridae -- South Africa , Insects as biological pest control agents -- South Africa
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/143046 , vital:38196
  • Description: Biological control involves the release of new species into the environment and therefore, needs to be carefully monitored through post-release assessments which have been largely neglected in the science. Post-release evaluations of biological control programmes reveal whether the control agent has established and if it impacts weed demography, while cost-benefit analyses require a different set of data that show the magnitude on return on investment. The biological control effort on Pontederia crassipes in South Africa uses, amongst others, two species of mirid, Eccritotarsus catarinensis and E. eichhorniae. Initially, they were released as a single species, but were recently divided using molecular techniques. Eccritotarsus catarinensis was released in 1999, and E. eichhorniae in 2007. After many releases over two decades, there was need to assess where each species was established in the country. Molecular techniques proved to be valuable in identifying the two species as they are morphologically indistinguishable in the field. Therefore, molecular techniques should be routinely used for screening biocontrol agents, whether new or as re-introductions. Annual surveys of the mirid release sites around South Africa were undertaken between 2016 and 2019. At each site both insect and plant parameters were measured. Only E. catarinensis is established in the field in South Africa despite the multiple releases of E. eichhorniae at over 70 sites across the country, and E. catarinensis has established at only 22 of the 45 release sites accessed during this study. This thesis tested climate, interaction with other agents already on P. crassipes, and direct competition between the two mirid species as reasons for the lack of establishment of E. eichhorniae. The results of the country-wide surveys showed that climate and water trophic status were the major determinants in the establishment of E. catarinensis. Most of the establishment was recorded in the warmer regions of the country, however, a few populations of the mirid also established in cooler areas, thus demonstrating a degree of thermal plasticity, and possible microclimates as the mirids persisted at sites shaded by riparian vegetation. Stochastic events such as active herbicide campaigns, winter frosts, droughts and floods were responsible for the absence of the mirid at some sites. At some of the eutrophic sites, despite the abundance of E. catarinensis, plants still proliferated as the water trophic status facilitated plant growth, thus, plants were able to compensate for the damage inflicted by the mirid. A more intensive, monthly, post-release evaluation was conducted on the Kubusi River, Eastern Cape Province between 2016 and 2019. This is regarded as one of the cooler water hyacinth sites. Populations of biological control agents at this site fluctuated seasonally. At this site, cold winters caused frosting of the leaves of P. crassipes with the exception of plants growing under overhanging vegetation that provided a refuge for the mirid. But, cool temperatures in the winter months (May to August) severely reduced the populations of E. catarinensis that required a long recovery phase in spring. The consequence of this was that the plants grew unchecked from the onset of the growing season forming dense mats. Of the four agents at the Kubusi River site, Eccritotarsus catarinensis recovered slowest after winter, with lag phases ranging from two months to several months of the three-year period. The release of a suite of agents has implications on the agents themselves, where interactions between the agents can be important. Interactions between pairs and even multiple agents can have implications for biocontrol, where agents are either complimentary or interfere with each other. In this case, because E. catarinensis recovered the slowest of the four agents at the site, plants were of a poor quality by mid-summer resulting in low mirid populations. Competition in weed biological control could be expected to be strongest between pairs of agents that share the same niche, and this could be the reason why E. eichhorniae failed to establish at sites where E. catarinensis had already been established for several years. When the two mirids were combined in manipulated trials in a polytunnel, populations were lower compared to when the two mirids occurred separately. Under warm conditions, it is likely that E. eichhorniae would be the superior agent compared to E. catarinensis. The evaluations discussed in this thesis highlighted gaps in agent release methodology in multispecies settings, as well as the need for strategic augmentation pre- and post-winter. It is important to release agents that will complement each other rather than compete, therefore, when releasing agents in a multispecies setting, niche differentiation needs to be considered. Here it is concluded that the best practice for dealing with the mirids is that they should be released individually, and at sites that have no other biological control agents in order to ultimately assess their efficacy. Landscape level, long-term monitoring of biological control programmes shows the impact of the control programme at a broader scale and, are far more informative than short-term studies and at fewer sites. Long-term post-release evaluations should be mandatory in biological control programmes. Furthermore, these assessments will help develop new strategies or improve on existing ones, thus achieve greater success in control.
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  • Date Issued: 2020

Post-apartheid nostalgia and the future of the black visual archive

  • Authors: Nsele, Zamansele Nsikakazi Busisiwe
  • Date: 2020
  • Subjects: Nostalgia in art , Memory in art , Africa -- In art , Africans in art , Fanon, Frantz, 1925-1961. Peau noire, masques blancs. English , South Africa -- In art , Black people in art
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/167177 , vital:41444
  • Description: The implications of nostalgia often strike a discordant note in post-apartheid discourse and this has opened up critical possibilities for research scholarship. For instance, Jacob Dlamini’s memoir Native Nostalgia entered the discursive fray in 2009, and it was subsequently followed by Derek Hook’s psychoanalytical approach in (Post) apartheid Conditions: Psychoanalysis and Social Formation in 2014. Notably, there is not yet a sustained and intensive research focus that has been conducted on post-apartheid forms of nostalgia within the discipline of art history and visual culture. I present this dissertation as a response to this gap. This thesis identifies mainly two competing nostalgias in post-apartheid South Africa. Through the analysis of selected artwork and media imagery, this dissertation critiques the connections of these nostalgias to the representation of the black figure in post-apartheid visual culture and the implications thereof. I argue that nostalgias for an apartheid-colonial-imperialist past operate through erasure and in the sanitisation of memory and as a result they render suffering indiscernible or in a sadomasochistic way consumes suffering as enjoyable. This thesis simultaneously critiques art work and visual representation that responds to South Africa’s nostalgia for the future: a restorative nostalgia that has emerged in the form of “rainbow nationalism”. This is a form of nostalgia that is underpinned by a dogged commitment to triumphalism and as a result erases the ongoing scenes of abjection. I use nostalgia and Afropessimism as analytical frameworks to argue that both real and visual representational forces work in tandem to restrain the future and this, I suggest is fulfilled by the transference of the black body from one state of unfreedom to next, resonating with a cyclical pattern. Frantz Fanon’s (1967) Black Skin White Mask forms the conceptual bedrock of my study, particularly his visual layout of “negrophobogenesis” and colonial temporality, which he describes as a “hellish cycle” or as an “infernal cycle” wherein the past overwhelms the present and ideas of the future.
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  • Date Issued: 2020

Quantifying ecosystem restoration recovery and restoration practice following the biological control of invasive alien macrophytes in Southern Africa

  • Authors: Motitsoe, Samuel Nkopane
  • Date: 2020
  • Subjects: Salvinia molesta , Ceratophyllum demersum , Nymphaea mexicana , Invasive plants -- Biological control -- South Africa , Aquatic weeds -- Biological control -- South Africa , Restoration monitoring (Ecology) -- South Africa , Biolotical invasions -- Environmental aspects
  • Language: English
  • Type: text , Thesis , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/167519 , vital:41488
  • Description: Invasive alien aquatic plants (IAAP) species are known to have deleterious effects on the freshwater ecosystems they invade. This includes both socio-economic and ecologically important ecosystem goods and services. Thus, IAAP species are declared a serious threat, second only to habitat modification for causing a loss of aquatic biodiversity. Three control methods have been widely applied to control IAAP species invasion globally; mechanical, chemical and biological control. Both mechanical and chemical control methods are considered short-term and expensive, whereas biological control methods are regarded an effective and long-term solution for IAAP species control at the landscape level. But, little is known of the ecological recovery following the biological control of IAAP species, with mechanical control known to have had mixed success and chemical control to have non-targeted effects on aquatic ecosystems, causing harm to wildlife and human well-being. Biological control practitioners measure the success of biological control based on: (1) the biological control agents’ establishment and the negative impacts they impose on the targeted weed; and (2) the weeds biomass reduction and an increase in native macrophytes species. Arguably, measures of biological control success have been subjective and variable across the globe. Although some field studies have demonstrated biological control success to have positive socio-economic returns, there is little literature on ecological benefits. Furthermore, there is limited understanding on ecosystem recovery and possible restoration efforts following the biological control IAAP species, as compared to alien weeds in terrestrial and riparian ecosystems. Thus, this thesis aimed to quantify the ecological recovery i.e. aquatic biodiversity, ecosystem processes and trophic interactions following the management of Salvinia molesta in freshwater ecosystems. The research employed a suite of Before-After Control-Impact mesocosm and field studies to investigate the response of aquatic microalgae, macroinvertebrates and their interactions (food web structure and function) during S. molesta infestation and after mechanical and biological control. The mesocosm experiment (Before invasion, During invasion & After control) showed that both aquatic microalgae and macroinvertebrate diversity indices were reliable biological indicators of S. molesta ecological impacts and recovery following control. The restored treatment (100% S. molesta cover + biological control agents), demonstrated complete aquatic microalgae and macroinvertebrate recovery following biological control, similar to the control treatment (open water), where the degraded/impacted treatment (100% S. molesta cover with no biological control agents) showed a drastic decline in aquatic biodiversity and a complete shift in aquatic biota assemblage structure. Thus, the biological control effort by Cyrtobagous salviniae, the biological control agent for S. molesta, assisted in the recovery of aquatic biota following successful biological control. The field study (four field sites, two sites controlled mechanically and two biologically) investigated water quality, aquatic biodiversity and community trophic interactions (aquatic food web) “before and after” S. molesta control. The study showed a drastic decline in aquatic biodiversity (with three sites showing no record of aquatic macroinvertebrates, thus no biotic interactions during infestation) and poor water quality due to the shade-effect (light barrier due to floating S. molesta mats on the water surface) during the “before” S. molesta control phase. However, following both mechanical and biological control (“after” S. molesta control phase), there was a significant shift in abiotic and biotic ecosystem characteristics as compared to the “before” S. molesta control phase. Thus, rapid ecosystem recovery was apparent as a result of aquatic microalgae and macroinvertebrates recolonisation. Sites showed a normal functioning ecosystem where improved water quality, increased biodiversity, productivity and trophic interactions, was indicative of the return of biologically and functionally important species which were lost during the “before” S. molesta phase. Although the clear water state showed positive outcomes at Westlake River, these were short lived when the system was dominated by a cosmopolitan submerged Ceratophyllum demersum, and later replaced by a floating-leaved emergent IAAP Nymphaea mexicana. Each state was responsible for a significant shift in both biotic and abiotic characteristics, affirming macrophyte abilities to influence aquatic environments structure and functions. Furthermore, this event showed a clear example of a secondary invasion. Thus, a holistic IAAP species management strategy is necessary to restore previously invaded ecosystems and prevent subsequent secondary invasion and ecosystem degradation. In conclusion, the S. molesta shade-effect like any other free-floating IAAP species, was identified as the main degrading factor and responsible for water quality reduction, loss of aquatic diversity and shift in aquatic biota assemblage structure. Following S. molesta removal (or shade-effect elimination), there was a positive response to aquatic ecosystem species abundance, richness, diversity and community structure. Therefore, in combination, aquatic biota recolonisation rate and increases in biological and functional diversity were instrumental in the recovery of ecosystem structure and functions, following the control of S. molesta. Echoing existing literature, this thesis recommends: (1) IAAP species management programmes (mechanical and/or biological control) should not only aim to control the weed but also focus on ecosystems recovery and possible restoration goals; (2) biological control should be used where appropriate to combat free-floating IAAP species in freshwater ecosystems, followed by active introduction of native macrophyte propagules since they are limited by anthropogenic activities; and (3) more freshwater case studies are needed to add to our understanding of IAAP species management and restoration effort incorporating long-term monitoring.
  • Full Text:
  • Date Issued: 2020
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