Network simulation for professional audio networks
- Authors: Otten, Fred
- Date: 2015
- Subjects: Sound engineers , Ethernet (Local area network system) , Computer networks , Computer simulation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4713 , http://hdl.handle.net/10962/d1017935
- Description: Audio Engineers are required to design and deploy large multi-channel sound systems which meet a set of requirements and use networking technologies such as Firewire and Ethernet AVB. Bandwidth utilisation and parameter groupings are among the factors which need to be considered in these designs. An implementation of an extensible, generic simulation framework would allow audio engineers to easily compare protocols and networking technologies and get near real time responses with regards to bandwidth utilisation. Our hypothesis is that an application-level capability can be developed which uses a network simulation framework to enable this process and enhances the audio engineer’s experience of designing and configuring a network. This thesis presents a new, extensible simulation framework which can be utilised to simulate professional audio networks. This framework is utilised to develop an application - AudioNetSim - based on the requirements of an audio engineer. The thesis describes the AudioNetSim models and implementations for Ethernet AVB, Firewire and the AES- 64 control protocol. AudioNetSim enables bandwidth usage determination for any network configuration and connection scenario and is used to compare Firewire and Ethernet AVB bandwidth utilisation. It also applies graph theory to the circular join problem and provides a solution to detect circular joins.
- Full Text:
- Authors: Otten, Fred
- Date: 2015
- Subjects: Sound engineers , Ethernet (Local area network system) , Computer networks , Computer simulation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4713 , http://hdl.handle.net/10962/d1017935
- Description: Audio Engineers are required to design and deploy large multi-channel sound systems which meet a set of requirements and use networking technologies such as Firewire and Ethernet AVB. Bandwidth utilisation and parameter groupings are among the factors which need to be considered in these designs. An implementation of an extensible, generic simulation framework would allow audio engineers to easily compare protocols and networking technologies and get near real time responses with regards to bandwidth utilisation. Our hypothesis is that an application-level capability can be developed which uses a network simulation framework to enable this process and enhances the audio engineer’s experience of designing and configuring a network. This thesis presents a new, extensible simulation framework which can be utilised to simulate professional audio networks. This framework is utilised to develop an application - AudioNetSim - based on the requirements of an audio engineer. The thesis describes the AudioNetSim models and implementations for Ethernet AVB, Firewire and the AES- 64 control protocol. AudioNetSim enables bandwidth usage determination for any network configuration and connection scenario and is used to compare Firewire and Ethernet AVB bandwidth utilisation. It also applies graph theory to the circular join problem and provides a solution to detect circular joins.
- Full Text:
Neurocognitive effects of head and body collisions on club level rugby union players
- Authors: Zoccola, Diana
- Date: 2015
- Subjects: Head -- Wounds and injuries -- Psychology , Neuropsychological tests , Head -- Wounds and injuries , Sports injuries -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3257 , http://hdl.handle.net/10962/d1016397
- Description: The objective of the study was to investigate the cumulative neurocognitive effects of repetitive concussive and subconcussive events in club level Rugby Union (hereafter rugby) during the course of one rugby season, in a combined group and individualized case-based approach. Amateur adult club level rugby players (n = 20) were compared with a non-contact control group (n = 22) of equivalent age, years of education and estimated IQ (p = > .05, in all instances), although the two groups were clearly differentiated on the basis of a history of reported concussions (p = < .05). Video analyses documented the tackling maneuvers observed amongst the players during all matches across the rugby season revealing a sobering average of more than a thousand tackles per player, excluding any contact practice sessions. Five rugby players (n = 5) who were observed to have a head jarring event were also isolated for individualized postconcussive follow-up analysis of their neurocognitive profiles. Measures included the ImPACT Verbal and Visual Memory, Visual Motor Speed and Reaction Time composites and the Purdue Pegboard. Independent and dependent statistical analyses were employed to compare the rugby versus control group neurocognitive test profiles at and between the three test intervals. Correlational analyses explored the association between concussion, tackling and neurocognitive test outcomes. Descriptive comparisons of individual neurocognitive test scores with normative data were employed for the case analyses. Taken together, the results implicated vulnerability amongst club rugby players on the motor and speeded tasks, with less robust indications on the memory tasks. While limited in terms of its small sample size, it is considered that the outcome of the study was rendered more robust by virtue of being methodologically multifaceted with heuristic implications for future research studies in the area. The novel inclusion of tackling data as well as fine-tuned case analyses, were of particular relevance in that regard. The results add to a growing body of literature that implicates deleterious neurocognitive effects in participants of a sport such as rugby due to repetitive head jarring incidents that are intrinsic to the game.
- Full Text:
- Authors: Zoccola, Diana
- Date: 2015
- Subjects: Head -- Wounds and injuries -- Psychology , Neuropsychological tests , Head -- Wounds and injuries , Sports injuries -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3257 , http://hdl.handle.net/10962/d1016397
- Description: The objective of the study was to investigate the cumulative neurocognitive effects of repetitive concussive and subconcussive events in club level Rugby Union (hereafter rugby) during the course of one rugby season, in a combined group and individualized case-based approach. Amateur adult club level rugby players (n = 20) were compared with a non-contact control group (n = 22) of equivalent age, years of education and estimated IQ (p = > .05, in all instances), although the two groups were clearly differentiated on the basis of a history of reported concussions (p = < .05). Video analyses documented the tackling maneuvers observed amongst the players during all matches across the rugby season revealing a sobering average of more than a thousand tackles per player, excluding any contact practice sessions. Five rugby players (n = 5) who were observed to have a head jarring event were also isolated for individualized postconcussive follow-up analysis of their neurocognitive profiles. Measures included the ImPACT Verbal and Visual Memory, Visual Motor Speed and Reaction Time composites and the Purdue Pegboard. Independent and dependent statistical analyses were employed to compare the rugby versus control group neurocognitive test profiles at and between the three test intervals. Correlational analyses explored the association between concussion, tackling and neurocognitive test outcomes. Descriptive comparisons of individual neurocognitive test scores with normative data were employed for the case analyses. Taken together, the results implicated vulnerability amongst club rugby players on the motor and speeded tasks, with less robust indications on the memory tasks. While limited in terms of its small sample size, it is considered that the outcome of the study was rendered more robust by virtue of being methodologically multifaceted with heuristic implications for future research studies in the area. The novel inclusion of tackling data as well as fine-tuned case analyses, were of particular relevance in that regard. The results add to a growing body of literature that implicates deleterious neurocognitive effects in participants of a sport such as rugby due to repetitive head jarring incidents that are intrinsic to the game.
- Full Text:
Nonlinear optical studies of phthalocyanines and their conjugates with nanomaterials
- Authors: Sanusi, Sikiru Olukayode
- Date: 2015
- Subjects: Phthalocyanines , Nonlinear optics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4545 , http://hdl.handle.net/10962/d1017925
- Description: A number of metallophthalocyanines (MPcs) and metal-free phthalocyanines (H₂Pcs) have been synthesized and characterized using various characterization tools such as ¹H-NMR, TOF mass spectrometry, FT-IR, UV-visible spectrophotometry and CHNS elemental analysis. Some of the MPcs were covalently linked to nanomaterials such as silica nanoparticles (SiNPs), single-walled carbon nanotubes (SWCNTs), magnetite nanoparticles (MNPs) and quantum dots (QDs), or embedded in polymer thin-films using poly(methyl methacrylate) (PMMA) and poly(acrylic acid) (PAA) as the polymer sources. The phthalocyanine-nanomaterial composites (Pc-NMCs) were characterized with FT-IR, UV-visible spectrophotometry, transmission electron microscopy (TEM), thermogravimetry analysis and X-ray diffractometry. The nonlinear optical (NLO) properties (using the open-aperture Z-scan technique) of the MPcs and the Pc-NMCs were investigated. In general, most of the investigated MPcs showed good optical limiting behaviors, except for a few, like the non-peripherally-substituted 2-pyridyloxy phthalocyanines, which showed inhibited NLO response as a result of the ring-strain effects. The absence of a metal center was found to greatly reduce the inherent high nonlinearities expected of some of the phthalocyanine complexes. The octaphenoxy derivatives (61a – 61e) were found to exhibit reverse saturable absorption (RSA) that depends on the singlet-singlet transitions, hence making them less reliable optical limiters. The optical limiting properties of the MPcs were improved in the presence of nanomaterials such as the QDs, MNPs and SWCNTs, with MPc-QDs showing the best optical limiting behavior of the three. SiNPs have no significant effect on the optical limiting behavior of the MPcs. The optical limiting properties of the MPcs were greatly enhanced in the presence of PMMA or PAA polymers. The PAA polymer showed better optical limiting behavior compared to PMMA
- Full Text:
- Authors: Sanusi, Sikiru Olukayode
- Date: 2015
- Subjects: Phthalocyanines , Nonlinear optics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4545 , http://hdl.handle.net/10962/d1017925
- Description: A number of metallophthalocyanines (MPcs) and metal-free phthalocyanines (H₂Pcs) have been synthesized and characterized using various characterization tools such as ¹H-NMR, TOF mass spectrometry, FT-IR, UV-visible spectrophotometry and CHNS elemental analysis. Some of the MPcs were covalently linked to nanomaterials such as silica nanoparticles (SiNPs), single-walled carbon nanotubes (SWCNTs), magnetite nanoparticles (MNPs) and quantum dots (QDs), or embedded in polymer thin-films using poly(methyl methacrylate) (PMMA) and poly(acrylic acid) (PAA) as the polymer sources. The phthalocyanine-nanomaterial composites (Pc-NMCs) were characterized with FT-IR, UV-visible spectrophotometry, transmission electron microscopy (TEM), thermogravimetry analysis and X-ray diffractometry. The nonlinear optical (NLO) properties (using the open-aperture Z-scan technique) of the MPcs and the Pc-NMCs were investigated. In general, most of the investigated MPcs showed good optical limiting behaviors, except for a few, like the non-peripherally-substituted 2-pyridyloxy phthalocyanines, which showed inhibited NLO response as a result of the ring-strain effects. The absence of a metal center was found to greatly reduce the inherent high nonlinearities expected of some of the phthalocyanine complexes. The octaphenoxy derivatives (61a – 61e) were found to exhibit reverse saturable absorption (RSA) that depends on the singlet-singlet transitions, hence making them less reliable optical limiters. The optical limiting properties of the MPcs were improved in the presence of nanomaterials such as the QDs, MNPs and SWCNTs, with MPc-QDs showing the best optical limiting behavior of the three. SiNPs have no significant effect on the optical limiting behavior of the MPcs. The optical limiting properties of the MPcs were greatly enhanced in the presence of PMMA or PAA polymers. The PAA polymer showed better optical limiting behavior compared to PMMA
- Full Text:
Performing whiteness; representing otherness : Hugh Tracey and African music
- Authors: Coetzee, Paulette June
- Date: 2015
- Subjects: Tracey, Hugh , Music -- Africa , International Library of African Music , Ethnomusicology -- Africa , Ethnomusicologists -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2327 , http://hdl.handle.net/10962/d1016502
- Description: This thesis provides a critical study of texts associated with Hugh Tracey (1903–1977). Tracey is well-known for his work in African music studies, particularly for his major contribution to the recorded archive of musical sound in sub-Saharan Africa and his founding of the International Library of African Music (ILAM) in 1954. My reading of him is informed by a postcolonial perspective, whiteness studies and African scholarship on ways in which constructions of African identity and tradition have been shaped by the colonial archive. In my view, Tracey was part of a mid-twentieth century movement which sought to marshal positive representations of traditional African culture in the interest of maintaining and strengthening colonial rule. While his recording project may have fostered inclusion through creating spaces for indigenous musicians to be heard, it also functioned to promote racist exclusion in the manner of its production, distribution and claims to expertise. Moreover, his initial strategy for ILAM’s sustainability targeted colonial government and industry as primary clients, with the promise that promoting traditional music as a means of entertainment and self-expression for black subjects and workers would ease administration and reduce conflict. I believe that it is important to acknowledge and interrogate the problematic racial attitudes and practices associated with the history of Tracey’s archive – not to undermine its significance in any way but to allow it to be better understood and used more productively in the future.
- Full Text:
- Authors: Coetzee, Paulette June
- Date: 2015
- Subjects: Tracey, Hugh , Music -- Africa , International Library of African Music , Ethnomusicology -- Africa , Ethnomusicologists -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2327 , http://hdl.handle.net/10962/d1016502
- Description: This thesis provides a critical study of texts associated with Hugh Tracey (1903–1977). Tracey is well-known for his work in African music studies, particularly for his major contribution to the recorded archive of musical sound in sub-Saharan Africa and his founding of the International Library of African Music (ILAM) in 1954. My reading of him is informed by a postcolonial perspective, whiteness studies and African scholarship on ways in which constructions of African identity and tradition have been shaped by the colonial archive. In my view, Tracey was part of a mid-twentieth century movement which sought to marshal positive representations of traditional African culture in the interest of maintaining and strengthening colonial rule. While his recording project may have fostered inclusion through creating spaces for indigenous musicians to be heard, it also functioned to promote racist exclusion in the manner of its production, distribution and claims to expertise. Moreover, his initial strategy for ILAM’s sustainability targeted colonial government and industry as primary clients, with the promise that promoting traditional music as a means of entertainment and self-expression for black subjects and workers would ease administration and reduce conflict. I believe that it is important to acknowledge and interrogate the problematic racial attitudes and practices associated with the history of Tracey’s archive – not to undermine its significance in any way but to allow it to be better understood and used more productively in the future.
- Full Text:
Photophysical studies of Zinc phthalocyanine-silica nanoparticles conjugates
- Authors: Fashina, Adedayo
- Date: 2015
- Subjects: Nanoparticles , Phthalocyanines , Zinc , Silica , Photochemistry , Adsorption
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4537 , http://hdl.handle.net/10962/d1017917
- Description: This thesis reports on the synthesis and characterization of both symmetrical and asymmetrical Zinc phthalocyanine complexes. The complexes contained groups such as carboxylic, amino and alkyne for covalent grafting to the surface of silica nanoparticles. The use of symmetrical and asymmetrical complexes was geared towards comparing the non-specific binding of the symmetrical complexes to the specific binding observed in the asymmetrical complexes. The complexes were also doped within the silica matrix and compared to the surface grafted conjugates. The complexes and the conjugates were well characterized with a variety of techniques. The fluorescence lifetimes of the phthalocyanine complexes containing either terminal carboxylic groups or an alkyne group showed a mono-exponential decay while the amino containing phthalocyanine complexes gave a bi-exponential decay. A similar trend was observed for their respective conjugates. Some of the conjugates of the asymmetrical complexes showed a decrease in fluorescence lifetimes and a corresponding decrease in fluorescence quantum yields. The fluorescence quantum yields for all the symmetrical complexes studied showed either an improvement or retained the luminescence of the grafted phthalocyanine complex. Most of the conjugates showed a faster intersystem crossing time in comparison to the complexes alone. The grafted or doped conjugates containing symmetrical phthalocyanine complexes with carboxyl groups showed improvements both in fluorescence and triplet quantum yields. All the conjugates except two showed an increase in triplet lifetimes when compared to their respective phthalocyanine complexes. Optical nonlinearities of nine of the phthalocyanine complexes were studied and all the complexes showed characteristic reverse saturable absorption behavior. Complex 10 showed the most promising optical limiting behavior. The aggregation and dissolution studies of the conjugates were also carried out in a simulated biological medium and the silicon level detected was noticed to have increased with incubation time.
- Full Text:
- Authors: Fashina, Adedayo
- Date: 2015
- Subjects: Nanoparticles , Phthalocyanines , Zinc , Silica , Photochemistry , Adsorption
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4537 , http://hdl.handle.net/10962/d1017917
- Description: This thesis reports on the synthesis and characterization of both symmetrical and asymmetrical Zinc phthalocyanine complexes. The complexes contained groups such as carboxylic, amino and alkyne for covalent grafting to the surface of silica nanoparticles. The use of symmetrical and asymmetrical complexes was geared towards comparing the non-specific binding of the symmetrical complexes to the specific binding observed in the asymmetrical complexes. The complexes were also doped within the silica matrix and compared to the surface grafted conjugates. The complexes and the conjugates were well characterized with a variety of techniques. The fluorescence lifetimes of the phthalocyanine complexes containing either terminal carboxylic groups or an alkyne group showed a mono-exponential decay while the amino containing phthalocyanine complexes gave a bi-exponential decay. A similar trend was observed for their respective conjugates. Some of the conjugates of the asymmetrical complexes showed a decrease in fluorescence lifetimes and a corresponding decrease in fluorescence quantum yields. The fluorescence quantum yields for all the symmetrical complexes studied showed either an improvement or retained the luminescence of the grafted phthalocyanine complex. Most of the conjugates showed a faster intersystem crossing time in comparison to the complexes alone. The grafted or doped conjugates containing symmetrical phthalocyanine complexes with carboxyl groups showed improvements both in fluorescence and triplet quantum yields. All the conjugates except two showed an increase in triplet lifetimes when compared to their respective phthalocyanine complexes. Optical nonlinearities of nine of the phthalocyanine complexes were studied and all the complexes showed characteristic reverse saturable absorption behavior. Complex 10 showed the most promising optical limiting behavior. The aggregation and dissolution studies of the conjugates were also carried out in a simulated biological medium and the silicon level detected was noticed to have increased with incubation time.
- Full Text:
Photophysicochemical properties and in vitro photodynamic therapy activities of zinc phthalocyanine conjugates with biomolecules and single-walled carbon nanotubes
- Authors: Ogbodu, Racheal O
- Date: 2015
- Subjects: Photochemotherapy , Phthalocyanines , Biomolecules
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4544 , http://hdl.handle.net/10962/d1017924
- Description: The synthesis, photophysicochemcial properties, in vitro dark toxicity and photodynamic therapy (PDT) activities of different derivatives of zinc phthalocyanine (ZnPc) conjugates with biomolecules (folic acid, bovine serum albumin (BSA), ascorbic acid, uridine or spermine) and single-walled carbon nanotubes (SWCNTs) are presented in this work. The fluorescence quantum yields (ΦF) (Subscript F) of the ZnPc derivatives or ZnPc-biomolecule conjugates remained relatively the same as compared to the precursor Pcs. Slight increases were observed in the ΦF (Subscript F) values of conjugates containing substituents such as pyrene, folic acid or BSA with intrinsic fluorescence properties. The triplet quantum yield (ΦT ) (Subscript T) values for some ZnPc conjugates increases compared to the precursor ZnPcs due to extended π conjugation (for the conjugate with pyrene) and the presence of phenyl ring that support spin-orbit charge transfer intersystem crossing to triplet state. While some conjugates showed decreases in the ΦT (Subscript T) values compared to precursor ZnPcs due to the presence of substituents that could quench photo-excited state properties. The singlet oxygen quantum yield (ΦΔ ) values follow the trends of the triplet quantum yield values. The conjugates containing BSA also show increases in the ΦΔ values without corresponding increases in ΦT (Subscript T) values due to the ability of BSA to generate free radicals including singlet oxygen. The presence of SWCNTs decreases the photophysicochemcial properties of some ZnPc-SWCNT conjugates compared to the precursor ZnPcs due to photo-induced electron transfer from an excited Pc complex (electron donor) to SWCNTs (electron acceptor). However, increases were observed in some ZnPc-SWCNT conjugates as a result of fast charge recombination process due to highly short-lived radical ion pair produced. These phenomena affected the ΦF (Suscript F) values, ΦT (Suscript T) values, and the ΦΔ values. Increases or decreases in ΦT (Suscript T) values resulted in corresponding increases or decreases in ΦΔ values
- Full Text:
- Authors: Ogbodu, Racheal O
- Date: 2015
- Subjects: Photochemotherapy , Phthalocyanines , Biomolecules
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4544 , http://hdl.handle.net/10962/d1017924
- Description: The synthesis, photophysicochemcial properties, in vitro dark toxicity and photodynamic therapy (PDT) activities of different derivatives of zinc phthalocyanine (ZnPc) conjugates with biomolecules (folic acid, bovine serum albumin (BSA), ascorbic acid, uridine or spermine) and single-walled carbon nanotubes (SWCNTs) are presented in this work. The fluorescence quantum yields (ΦF) (Subscript F) of the ZnPc derivatives or ZnPc-biomolecule conjugates remained relatively the same as compared to the precursor Pcs. Slight increases were observed in the ΦF (Subscript F) values of conjugates containing substituents such as pyrene, folic acid or BSA with intrinsic fluorescence properties. The triplet quantum yield (ΦT ) (Subscript T) values for some ZnPc conjugates increases compared to the precursor ZnPcs due to extended π conjugation (for the conjugate with pyrene) and the presence of phenyl ring that support spin-orbit charge transfer intersystem crossing to triplet state. While some conjugates showed decreases in the ΦT (Subscript T) values compared to precursor ZnPcs due to the presence of substituents that could quench photo-excited state properties. The singlet oxygen quantum yield (ΦΔ ) values follow the trends of the triplet quantum yield values. The conjugates containing BSA also show increases in the ΦΔ values without corresponding increases in ΦT (Subscript T) values due to the ability of BSA to generate free radicals including singlet oxygen. The presence of SWCNTs decreases the photophysicochemcial properties of some ZnPc-SWCNT conjugates compared to the precursor ZnPcs due to photo-induced electron transfer from an excited Pc complex (electron donor) to SWCNTs (electron acceptor). However, increases were observed in some ZnPc-SWCNT conjugates as a result of fast charge recombination process due to highly short-lived radical ion pair produced. These phenomena affected the ΦF (Suscript F) values, ΦT (Suscript T) values, and the ΦΔ values. Increases or decreases in ΦT (Suscript T) values resulted in corresponding increases or decreases in ΦΔ values
- Full Text:
Phthalocyanine-nanoparticle conjugates for photodynamic therapy of cancer and phototransformation of organic pollutants
- Authors: Khoza, Phindile Brenda
- Date: 2015
- Subjects: Phthalocyanines , Nanoparticles , Photochemotherapy , Cancer -- Chemotherapy , Zinc oxide , Photocatalysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4538 , http://hdl.handle.net/10962/d1017918
- Description: The synthesis and extensive spectroscopical characterization of novel phthalocyanines are reported. The new compounds were characterized by elemental analysis, FT-IR, ¹HNMR, mass spectrometry and UV–Vis spectroscopy. The new phthalocyanines showed remarkable photophysicochemical behaviour. The novel phthalocyanines were then conjugated to nanoparticles, silver and ZnO. The coupling of the novel Pcs to nanoparticles was through covalent bonding and ligand exchange. These conjugates were supported onto electrospun polystyrene fibers and chitosan microbeads for use as photocatalysts. The efficiency of the immobilized Pcs and Pc-nanoparticles was assessed by the phototrasfromation of organic pollutants, methyl orange and Rhodamine 6G as model dyes. Upon conjugating phthalocyanines to nanoparticles, there was a great increase in the rate of photodegradation of the model dyes. The photodynamic activity of the novel phthalocyanines upon conjugating to nanoparticles and selected targeting agents is also reported. The targeting agents employed in this study are folic acid and polylysine. Conjugating the phthalocyanines to folic acid or polylysine improved the solubility of the phthalocyanines in aqueous media. The potency of the conjugates was investigated on breast (MCF-7), prostate and melanoma cancer cell lines. The phthalocyanines showed no toxicity in the absence of light. However, upon illumination, a concentration dependent cellular decrease was observed.
- Full Text:
- Authors: Khoza, Phindile Brenda
- Date: 2015
- Subjects: Phthalocyanines , Nanoparticles , Photochemotherapy , Cancer -- Chemotherapy , Zinc oxide , Photocatalysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4538 , http://hdl.handle.net/10962/d1017918
- Description: The synthesis and extensive spectroscopical characterization of novel phthalocyanines are reported. The new compounds were characterized by elemental analysis, FT-IR, ¹HNMR, mass spectrometry and UV–Vis spectroscopy. The new phthalocyanines showed remarkable photophysicochemical behaviour. The novel phthalocyanines were then conjugated to nanoparticles, silver and ZnO. The coupling of the novel Pcs to nanoparticles was through covalent bonding and ligand exchange. These conjugates were supported onto electrospun polystyrene fibers and chitosan microbeads for use as photocatalysts. The efficiency of the immobilized Pcs and Pc-nanoparticles was assessed by the phototrasfromation of organic pollutants, methyl orange and Rhodamine 6G as model dyes. Upon conjugating phthalocyanines to nanoparticles, there was a great increase in the rate of photodegradation of the model dyes. The photodynamic activity of the novel phthalocyanines upon conjugating to nanoparticles and selected targeting agents is also reported. The targeting agents employed in this study are folic acid and polylysine. Conjugating the phthalocyanines to folic acid or polylysine improved the solubility of the phthalocyanines in aqueous media. The potency of the conjugates was investigated on breast (MCF-7), prostate and melanoma cancer cell lines. The phthalocyanines showed no toxicity in the absence of light. However, upon illumination, a concentration dependent cellular decrease was observed.
- Full Text:
Pitied plumage and dying birds : the public mourning of national heroines and post-apartheid foundational mythology construction
- Authors: Kerseboom, Simone
- Date: 2015
- Subjects: Women heroes -- South Africa , Nationalism -- South Africa , Nationalism and collective memory -- South Africa , Post-apartheid era -- South Africa , Women political activists -- South Africa , Dead -- Political aspects -- South Africa , Critical discourse analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2625 , http://hdl.handle.net/10962/d1019884
- Description: The original contribution of this thesis is the examination of the official construction of a post-apartheid foundation myth through the analysis of the dead body politics of five iconic South African women that spans the three presidencies that have defined South Africa’s democratic era. This thesis examines the death and funeral of Albertina Sisulu, the return and burial of Sara Baartman, and the commemoration of Charlotte Maxeke, Lilian Ngoyi, and Helen Joseph. Sisulu, Baartman, Maxeke, Ngoyi, and Joseph have been constructed as heroines and as foundational figures for the post-apartheid nation in official rhetoric. It will contend that the dead body politics of these women not only informs a new foundational mythology, but also features in the processes of regime legitimation when the ANC-dominated government faces strong societal criticism. Although such official expressions of nationalism may appear exhausted, this thesis will show that nationalism remains a powerful and dangerous force in South Africa that attempts to silence opposition and critical analysis of perceived failing government policies or inaction. This thesis will indicate that as women’s bodies and legacies are appropriated for nationalist projects they are subsumed in discourses of domestic femininity in official rhetoric that dangerously detract from women’s democratic rights and their ability to exercise responsible and productive citizenship in the post-apartheid state. It will argue that women’s historic political activism is contained within the meta-narrative of ‘The Struggle’ and that women are re-subsumed into the patriarchal discourses of the past that are inherited in the present. This thesis approaches this topic by considering a top-to-bottom construction of post-apartheid nationalism through applying feminist critical discourse analysis to official rhetoric articulated at the public mourning and commemorative rituals of these five women.
- Full Text:
- Authors: Kerseboom, Simone
- Date: 2015
- Subjects: Women heroes -- South Africa , Nationalism -- South Africa , Nationalism and collective memory -- South Africa , Post-apartheid era -- South Africa , Women political activists -- South Africa , Dead -- Political aspects -- South Africa , Critical discourse analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2625 , http://hdl.handle.net/10962/d1019884
- Description: The original contribution of this thesis is the examination of the official construction of a post-apartheid foundation myth through the analysis of the dead body politics of five iconic South African women that spans the three presidencies that have defined South Africa’s democratic era. This thesis examines the death and funeral of Albertina Sisulu, the return and burial of Sara Baartman, and the commemoration of Charlotte Maxeke, Lilian Ngoyi, and Helen Joseph. Sisulu, Baartman, Maxeke, Ngoyi, and Joseph have been constructed as heroines and as foundational figures for the post-apartheid nation in official rhetoric. It will contend that the dead body politics of these women not only informs a new foundational mythology, but also features in the processes of regime legitimation when the ANC-dominated government faces strong societal criticism. Although such official expressions of nationalism may appear exhausted, this thesis will show that nationalism remains a powerful and dangerous force in South Africa that attempts to silence opposition and critical analysis of perceived failing government policies or inaction. This thesis will indicate that as women’s bodies and legacies are appropriated for nationalist projects they are subsumed in discourses of domestic femininity in official rhetoric that dangerously detract from women’s democratic rights and their ability to exercise responsible and productive citizenship in the post-apartheid state. It will argue that women’s historic political activism is contained within the meta-narrative of ‘The Struggle’ and that women are re-subsumed into the patriarchal discourses of the past that are inherited in the present. This thesis approaches this topic by considering a top-to-bottom construction of post-apartheid nationalism through applying feminist critical discourse analysis to official rhetoric articulated at the public mourning and commemorative rituals of these five women.
- Full Text:
Primary maths teacher learning and identity within a numeracy in-service community of practice
- Authors: Pausigere, Peter
- Date: 2015
- Subjects: Mathematics -- Study and teaching (Elementary) -- South Africa , Teachers -- In-service training -- South Africa , Student-centered learning -- South Africa , Communities of practice -- South Africa , Educational change -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2019 , http://hdl.handle.net/10962/d1017183
- Description: This study focuses on the processes of primary maths teacher learning and how their identities and practices evolve in relation to participation in a primary maths focused in-service teacher education programme, called the Numeracy Inquiry Community of Leader Educators (NICLE).Additionally it investigates activities, relations and forms of participation within the Community of Practice (CoP) which enable or constrain evolving primary maths identities and practices and how these relate to the broader context. The study draws from the situative-participationists (Lave, 1996; Wenger, 1998; Sfard & Prusak, 2005; Wenger et al, 2002) theoretical framework supplemented by Bernstein’s (2000) pedagogic identity model. Using a qualitative educational interpretive approach I sampled 8 primary teachers drawn from NICLE and gathered data through participant observations, interactive interviews, document analysis and reflective journals. Analysing the key data themes that emerged from teacher learning stories, which I have called stelos, the study explains the nature of the primary maths teachers’ learning, transformation and participation experiences in NICLE using the synonyms reinvigoration and remediation and activation and relating these semantics to the teachers’ mathematical identities and histories. The study also explains the processes through which primary maths teacher identities evolve in relation to participation in an in-service CoP as ‘insiding’ and ‘outcropping’. Interpreting qualitative data from the empirical field indicates that teachers participating in NICLE mostly took-up into their maths classrooms key numeracy-domain concepts, resources and issues presented by primary maths experts which are informed by research and theory that link to practices. Teachers collaboratively and actively engaged in a range of activities that relate to classroom practices. Teacher learning was also enabled when teachers engaged in maths overlapping communities of practice, shared classroom experiences in friendly ways with fellow NICLE teachers and engaged with NICLE presenters who mutually respected and regarded them as professionals. Such affordances were said to enable teachers to engage learners in maths classes and improve their understanding of specific primary maths concepts. On the other hand teachers felt challenged by the travelling distance, limited time and also raised the tension of how to scale-up maths professional development initiatives to include schools from their community. The study makes a theoretical contribution by illustrating how Bernstein’s pedagogic identity model and its elaboration by Tyler (1999) provides analytical tools to interrogate macro educational changes and connect these to the micro processes and teacher identities.
- Full Text:
- Authors: Pausigere, Peter
- Date: 2015
- Subjects: Mathematics -- Study and teaching (Elementary) -- South Africa , Teachers -- In-service training -- South Africa , Student-centered learning -- South Africa , Communities of practice -- South Africa , Educational change -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2019 , http://hdl.handle.net/10962/d1017183
- Description: This study focuses on the processes of primary maths teacher learning and how their identities and practices evolve in relation to participation in a primary maths focused in-service teacher education programme, called the Numeracy Inquiry Community of Leader Educators (NICLE).Additionally it investigates activities, relations and forms of participation within the Community of Practice (CoP) which enable or constrain evolving primary maths identities and practices and how these relate to the broader context. The study draws from the situative-participationists (Lave, 1996; Wenger, 1998; Sfard & Prusak, 2005; Wenger et al, 2002) theoretical framework supplemented by Bernstein’s (2000) pedagogic identity model. Using a qualitative educational interpretive approach I sampled 8 primary teachers drawn from NICLE and gathered data through participant observations, interactive interviews, document analysis and reflective journals. Analysing the key data themes that emerged from teacher learning stories, which I have called stelos, the study explains the nature of the primary maths teachers’ learning, transformation and participation experiences in NICLE using the synonyms reinvigoration and remediation and activation and relating these semantics to the teachers’ mathematical identities and histories. The study also explains the processes through which primary maths teacher identities evolve in relation to participation in an in-service CoP as ‘insiding’ and ‘outcropping’. Interpreting qualitative data from the empirical field indicates that teachers participating in NICLE mostly took-up into their maths classrooms key numeracy-domain concepts, resources and issues presented by primary maths experts which are informed by research and theory that link to practices. Teachers collaboratively and actively engaged in a range of activities that relate to classroom practices. Teacher learning was also enabled when teachers engaged in maths overlapping communities of practice, shared classroom experiences in friendly ways with fellow NICLE teachers and engaged with NICLE presenters who mutually respected and regarded them as professionals. Such affordances were said to enable teachers to engage learners in maths classes and improve their understanding of specific primary maths concepts. On the other hand teachers felt challenged by the travelling distance, limited time and also raised the tension of how to scale-up maths professional development initiatives to include schools from their community. The study makes a theoretical contribution by illustrating how Bernstein’s pedagogic identity model and its elaboration by Tyler (1999) provides analytical tools to interrogate macro educational changes and connect these to the micro processes and teacher identities.
- Full Text:
Pro-active visualization of cyber security on a National Level : a South African case study
- Authors: Swart, Ignatius Petrus
- Date: 2015
- Subjects: Internet -- Security measures -- South Africa , Computer security -- Government policy -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4718 , http://hdl.handle.net/10962/d1017940
- Description: The need for increased national cyber security situational awareness is evident from the growing number of published national cyber security strategies. Governments are progressively seen as responsible for cyber security, but at the same time increasingly constrained by legal, privacy and resource considerations. Infrastructure and services that form part of the national cyber domain are often not under the control of government, necessitating the need for information sharing between governments and commercial partners. While sharing of security information is necessary, it typically requires considerable time to be implemented effectively. In an effort to decrease the time and effort required for cyber security situational awareness, this study considered commercially available data sources relating to a national cyber domain. Open source information is typically used by attackers to gather information with great success. An understanding of the data provided by these sources can also afford decision makers the opportunity to set priorities more effectively. Through the use of an adapted Joint Directors of Laboratories (JDL) fusion model, an experimental system was implemented that visualized the potential that open source intelligence could have on cyber situational awareness. Datasets used in the validation of the model contained information obtained from eight different data sources over a two year period with a focus on the South African .co.za sub domain. Over a million infrastructure devices were examined in this study along with information pertaining to a potential 88 million vulnerabilities on these devices. During the examination of data sources, a severe lack of information regarding the human aspect in cyber security was identified that led to the creation of a novel Personally Identifiable Information detection sensor (PII). The resultant two million records pertaining to PII in the South African domain were incorporated into the data fusion experiment for processing. The results of this processing are discussed in the three case studies. The results offered in this study aim to highlight how data fusion and effective visualization can serve to move national cyber security from a primarily reactive undertaking to a more pro-active model.
- Full Text:
- Authors: Swart, Ignatius Petrus
- Date: 2015
- Subjects: Internet -- Security measures -- South Africa , Computer security -- Government policy -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4718 , http://hdl.handle.net/10962/d1017940
- Description: The need for increased national cyber security situational awareness is evident from the growing number of published national cyber security strategies. Governments are progressively seen as responsible for cyber security, but at the same time increasingly constrained by legal, privacy and resource considerations. Infrastructure and services that form part of the national cyber domain are often not under the control of government, necessitating the need for information sharing between governments and commercial partners. While sharing of security information is necessary, it typically requires considerable time to be implemented effectively. In an effort to decrease the time and effort required for cyber security situational awareness, this study considered commercially available data sources relating to a national cyber domain. Open source information is typically used by attackers to gather information with great success. An understanding of the data provided by these sources can also afford decision makers the opportunity to set priorities more effectively. Through the use of an adapted Joint Directors of Laboratories (JDL) fusion model, an experimental system was implemented that visualized the potential that open source intelligence could have on cyber situational awareness. Datasets used in the validation of the model contained information obtained from eight different data sources over a two year period with a focus on the South African .co.za sub domain. Over a million infrastructure devices were examined in this study along with information pertaining to a potential 88 million vulnerabilities on these devices. During the examination of data sources, a severe lack of information regarding the human aspect in cyber security was identified that led to the creation of a novel Personally Identifiable Information detection sensor (PII). The resultant two million records pertaining to PII in the South African domain were incorporated into the data fusion experiment for processing. The results of this processing are discussed in the three case studies. The results offered in this study aim to highlight how data fusion and effective visualization can serve to move national cyber security from a primarily reactive undertaking to a more pro-active model.
- Full Text:
Production of Cydia pomonella granulovirus (CpGV) in a heteralogous host, Thaumatotibia Leucotreta (Meyrick) (False codling moth)
- Authors: Chambers, Craig Brian
- Date: 2015
- Subjects: Cryptophlebia leucotreta -- South Africa , Codling moth -- South Africa , Apples -- Diseases and pests -- South Africa , Codling moth -- Biological control -- South Africa , Insect pests -- Biological control -- South Africa , Biological pest control agents -- South Africa , Baculoviruses -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5935 , http://hdl.handle.net/10962/d1017906
- Description: Cydia pomonella (Linnaeus) (Family: Tortricidae), the codling moth, is considered one of the most significant pests of apples and pears worldwide, causing up to 80% crop loss in orchards if no control measures are applied. Cydia pomonella is oligophagous feeding on a number of alternate hosts including quince, walnuts, apricots, peaches, plums and nectarines. Historically the control of this pest has been achieved with the use of various chemical control strategies which have maintained pest levels below the economic threshold at a relatively low cost to the grower. However, there are serious concerns surrounding the use of chemical insecticides including the development of resistance in insect populations, the banning of various insecticides, regulations for lowering of the maximum residue level and employee and consumer safety. For this reason, alternate measures of control are slowly being adopted by growers such as mating disruption, cultural methods and the use of baculovirus biopesticides as part of integrated pest management programmes. The reluctance of growers to accept baculovirus or other biological control products in the past has been due to questionable product quality and inconsistencies in their field performance. Moreover, the development and application of biological control products is more costly than the use of chemical alternatives. Baculoviruses are arthropod specific viruses that are highly virulent to a number of lepidopteran species. Due to the virulence and host specificity of baculoviruses, Cydia pomonella granulovirus has been extensively and successfully used as part of integrated pest management systems for the control of C. pomonella in Europe and around the world, including South Africa. Commercial formulations have been typically based on the Mexican strain of CpGV. However due to long-term multiple applications of CpGV and the reliance on CpGV in organic farming practices in Europe, resistance to the CpGV-M strain has developed in a number of field populations of C. pomonella. This study aimed to identify and characterize novel isolates of CpGV in South Africa and compare their virulence with the commercial standard CpGV-M. Secondly, since C. pomonella is difficult to culture on a large scale, an alternate method of CpGV production was investigated in order to determine if CpGV could be produced more efficiently and at a reduced cost without negatively impacting the quality of the product. Several isolates of CpGV were recovered either from field collected larvae or from a laboratory-reared C. pomonella colony. Characterisation of DNA profiles using a variety of restriction enzymes revealed that only a single isolate, CpGV-SA, was genetically different from the Mexican strain of the virus used in the commercially available CpGV based products in South Africa. In dose-response bioassays using CpGV-SA, LC₅₀ and LC₉₀ values for neonate C. pomonella larvae were 3.18 x 10³ OBs/ml and 7.33 x 10⁴ respectively. A comparison of these values with those of CpGV-M indicated no significant difference in the virulence of the two isolates under laboratory conditions. This is a first report of a genetically distinct CpGV isolate in South Africa. The biological activity and novelty of CpGV-SA makes this isolate a potentially important tool for CpGV resistance management in South Africa. In order to justify production of CpGV in an alternative host, studies on the comparative biological performance of C. pomonella and T. leucotreta based on oviposition, time to hatch, larval developmental times and rearing efficiency as well as production costs were performed. Thaumatotibia leucotreta was found to be more fecund and to have significantly shorter egg and larval developmental times. In addition, larval production per unit of artificial diet was significantly higher than for C. pomonella. This resulted in T. leucotreta being more cost effective to produce with implications for reduced insectary space, sanitation practices as well as the labour component of production. Virus yield data generated by inoculation both C. pomonella and T. leucotreta with nine concentrations of CpGV resulted in comparable virus yields, justifying the continuation of the research into production of CpGV in T. leucotreta. It was important to determine the LC and LT values required for mass production of CpGV in late instar T. leucotreta larvae. Dose- and time-response bioassays with CpGV-M were conducted on artificial diet to determine these values. Fourth instar LC₅₀ and LC₉₀ values were 5.96 x 10³ OBs/ml and 1.64 x 10⁵ OBs/ml respectively. LT50 and LT90 values were 81.10 hours and 88.58 hours respectively. Fifth instar LC₅₀ and LC₉₀ values were 6.88 x 10⁴ OBs/ml and 9.78 x 10⁶ OBs/ml respectively. LT₅₀ and LT₉₀ values were 111.56 hours and 137.57 hours respectively. Virus produced in fourth instar T. leucotreta larvae was bioassayed against C. pomonella neonate larvae and compared to CpGV-M to establish if production in the heterologous host negatively affected the virulence of the isolate. No significant difference in virulence was observed between virus produced in T. leucotreta and that produced in C. pomonella. The data generated in the bioassays was used in CpGV mass production trials to evaluate production. All production methods tested produced acceptable virus yields. To examine the quality of the virus product, genomic DNA was extracted from larval cadavers and subjected to REN analysis with HindIII. The resulting DNA profiles indicated that the virus product was contaminated with the homologous virus, CrleGV. Based on the above results, the use of T. leucotreta as an alternate host for the in vivo production of CpGV on a commercial basis is not at this stage viable and requires further investigation before this production methodology can be reliable used to produce CpGV. However, this study has shown that CpGV can be produced in a homologous host, T. leucotreta and significant strides have been made towards developing a set of quality control standards that are essential for further development of successful production methodology. Finally a novel isolate of CpGV has been identified with comparable virulence to the CpGV-M. This is an important finding as it has broad reaching implications for resistance management of CpGV products in South Africa.
- Full Text:
- Authors: Chambers, Craig Brian
- Date: 2015
- Subjects: Cryptophlebia leucotreta -- South Africa , Codling moth -- South Africa , Apples -- Diseases and pests -- South Africa , Codling moth -- Biological control -- South Africa , Insect pests -- Biological control -- South Africa , Biological pest control agents -- South Africa , Baculoviruses -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5935 , http://hdl.handle.net/10962/d1017906
- Description: Cydia pomonella (Linnaeus) (Family: Tortricidae), the codling moth, is considered one of the most significant pests of apples and pears worldwide, causing up to 80% crop loss in orchards if no control measures are applied. Cydia pomonella is oligophagous feeding on a number of alternate hosts including quince, walnuts, apricots, peaches, plums and nectarines. Historically the control of this pest has been achieved with the use of various chemical control strategies which have maintained pest levels below the economic threshold at a relatively low cost to the grower. However, there are serious concerns surrounding the use of chemical insecticides including the development of resistance in insect populations, the banning of various insecticides, regulations for lowering of the maximum residue level and employee and consumer safety. For this reason, alternate measures of control are slowly being adopted by growers such as mating disruption, cultural methods and the use of baculovirus biopesticides as part of integrated pest management programmes. The reluctance of growers to accept baculovirus or other biological control products in the past has been due to questionable product quality and inconsistencies in their field performance. Moreover, the development and application of biological control products is more costly than the use of chemical alternatives. Baculoviruses are arthropod specific viruses that are highly virulent to a number of lepidopteran species. Due to the virulence and host specificity of baculoviruses, Cydia pomonella granulovirus has been extensively and successfully used as part of integrated pest management systems for the control of C. pomonella in Europe and around the world, including South Africa. Commercial formulations have been typically based on the Mexican strain of CpGV. However due to long-term multiple applications of CpGV and the reliance on CpGV in organic farming practices in Europe, resistance to the CpGV-M strain has developed in a number of field populations of C. pomonella. This study aimed to identify and characterize novel isolates of CpGV in South Africa and compare their virulence with the commercial standard CpGV-M. Secondly, since C. pomonella is difficult to culture on a large scale, an alternate method of CpGV production was investigated in order to determine if CpGV could be produced more efficiently and at a reduced cost without negatively impacting the quality of the product. Several isolates of CpGV were recovered either from field collected larvae or from a laboratory-reared C. pomonella colony. Characterisation of DNA profiles using a variety of restriction enzymes revealed that only a single isolate, CpGV-SA, was genetically different from the Mexican strain of the virus used in the commercially available CpGV based products in South Africa. In dose-response bioassays using CpGV-SA, LC₅₀ and LC₉₀ values for neonate C. pomonella larvae were 3.18 x 10³ OBs/ml and 7.33 x 10⁴ respectively. A comparison of these values with those of CpGV-M indicated no significant difference in the virulence of the two isolates under laboratory conditions. This is a first report of a genetically distinct CpGV isolate in South Africa. The biological activity and novelty of CpGV-SA makes this isolate a potentially important tool for CpGV resistance management in South Africa. In order to justify production of CpGV in an alternative host, studies on the comparative biological performance of C. pomonella and T. leucotreta based on oviposition, time to hatch, larval developmental times and rearing efficiency as well as production costs were performed. Thaumatotibia leucotreta was found to be more fecund and to have significantly shorter egg and larval developmental times. In addition, larval production per unit of artificial diet was significantly higher than for C. pomonella. This resulted in T. leucotreta being more cost effective to produce with implications for reduced insectary space, sanitation practices as well as the labour component of production. Virus yield data generated by inoculation both C. pomonella and T. leucotreta with nine concentrations of CpGV resulted in comparable virus yields, justifying the continuation of the research into production of CpGV in T. leucotreta. It was important to determine the LC and LT values required for mass production of CpGV in late instar T. leucotreta larvae. Dose- and time-response bioassays with CpGV-M were conducted on artificial diet to determine these values. Fourth instar LC₅₀ and LC₉₀ values were 5.96 x 10³ OBs/ml and 1.64 x 10⁵ OBs/ml respectively. LT50 and LT90 values were 81.10 hours and 88.58 hours respectively. Fifth instar LC₅₀ and LC₉₀ values were 6.88 x 10⁴ OBs/ml and 9.78 x 10⁶ OBs/ml respectively. LT₅₀ and LT₉₀ values were 111.56 hours and 137.57 hours respectively. Virus produced in fourth instar T. leucotreta larvae was bioassayed against C. pomonella neonate larvae and compared to CpGV-M to establish if production in the heterologous host negatively affected the virulence of the isolate. No significant difference in virulence was observed between virus produced in T. leucotreta and that produced in C. pomonella. The data generated in the bioassays was used in CpGV mass production trials to evaluate production. All production methods tested produced acceptable virus yields. To examine the quality of the virus product, genomic DNA was extracted from larval cadavers and subjected to REN analysis with HindIII. The resulting DNA profiles indicated that the virus product was contaminated with the homologous virus, CrleGV. Based on the above results, the use of T. leucotreta as an alternate host for the in vivo production of CpGV on a commercial basis is not at this stage viable and requires further investigation before this production methodology can be reliable used to produce CpGV. However, this study has shown that CpGV can be produced in a homologous host, T. leucotreta and significant strides have been made towards developing a set of quality control standards that are essential for further development of successful production methodology. Finally a novel isolate of CpGV has been identified with comparable virulence to the CpGV-M. This is an important finding as it has broad reaching implications for resistance management of CpGV products in South Africa.
- Full Text:
Re-imagining the nation
- Mngomezulu, Nosipho Sthabiso Thandiwe
- Authors: Mngomezulu, Nosipho Sthabiso Thandiwe
- Date: 2015
- Subjects: Mauritius -- Truth and Justice Commission , Nationalism -- Mauritius , National characteristics, Mauritian , Social justice -- Mauritius , Youth -- Mauritius -- Social conditions , Youth -- Mauritius -- Attitudes , Mauritius -- Politics and government -- 1992- , Ethnicity -- Mauritius
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2124 , http://hdl.handle.net/10962/d1019999
- Description: This thesis examines young people’s constructions of nationhood in Mauritius. In 2008, the Mauritian government instituted a Truth and Justice Commission (TJC), set up to investigate the consequences of slavery and indentured labour. Through the Truth and Justice Commission, the Mauritian government indicated its desire to achieve social justice and national unity. Drawing on developments in studies of national identification practices in the 21st Century, this thesis addresses the question of young Mauritian’s locally and globally informed identification practices and asks how their unofficial narratives of nationhood challenge, or divert, or relate to official state narratives of nationhood. The basis of the study emerges from data collected from 132 participants during fieldwork in multiple fieldsites from May to September 2010 as well as research on Mauritian youth on-line from 2011-2014. The advent of the TJC offers an ideal moment to evaluate the dynamics of post-colonial nation-building and nationhood in a selfstyled multi-cultural state. Nationhood, does not exist apriori to the constructions of narratives of the nation, thus the stories told about the nation, imagine the nation into being. By situating the Truth and Justice Commission and other official state narratives alongside young people’s narratives, I argue that contemporary narratives of nationhood in Mauritius represent an intergenerational struggle to define the meaning of the past in the present and consequently outline the future. Reflecting on the ideas and socio-economic and political processes that induce national consciousness, I argue that young people’s narratives of everyday lived experiences are vital for an interpretation of how nationhood is produced in everyday life. The cultural projects of young people – often rendered as liminal or marginal – offer a critical vantage point from where to read constructions of nationhood. Far from being growing pains or childish games, young people’s identity making practices are what Sherry B. Ortner has called “serious games.” This research suggests that official state government narratives of multicultural nationhood in Mauritius narrowly define national identification along communal loyalties, overlooking the dynamism of interculturality and transnationalism in daily practice on the island. Although communalism and rigid colonial interpretations of ethnicity attempt to police and limit the possibilities of alternative modes of being in Mauritius, young people’s identification practices question, challenge, and threaten to disrupt official discourses of ethnic identification in Mauritius Scholarly investigations of young peoples’ lived experiences of nationhood extend theoretical and methodological frames for the study of nationalized subjects and deepen the understanding of the construction of national consciousness. The construction of nationhood always involves narratives of some sort – scholarship on this area has usually focused on official state narratives from social theorists, state governments, and state elites. I argue for the importance of considering subjectivity and lived experience in conceptions of nationhood. In contemporary post-colonial societies, young people are the numerical majority, however, their voices are seldom represented in theories and narratives of nationhood. Whilst young people may appear in state policies (especially education) and official narratives about the future of the nation, their creative imagining and reimagining of narratives of selfhood is often ignored. I examine how young people increasingly are aware of their transnational connections, through participation in transnational youth cultures, and they are consequently increasingly multi-lingual and multicultural. Fixed notions of ethnic identification and discourses of trauma are not at the forefront of young people’s identification of selfhood, rather their ability to take advantage of their multiply situated identification processes allows them new means to evade and transform these narratives. Their identification of selfhood is characterised by a greater degree of dynamism than previous generations had access to, and thus they do not only identify themselves through officially sanctioned national forms of identification. Loyalty to nationhood is thus less predictable, and young people represent a potential threat to the continuation of older forms of nationhood. While official narratives of nationhood may manipulate ethnic and racial cleavages to secure old loyalties, not all young people are persuaded by these notions
- Full Text:
- Authors: Mngomezulu, Nosipho Sthabiso Thandiwe
- Date: 2015
- Subjects: Mauritius -- Truth and Justice Commission , Nationalism -- Mauritius , National characteristics, Mauritian , Social justice -- Mauritius , Youth -- Mauritius -- Social conditions , Youth -- Mauritius -- Attitudes , Mauritius -- Politics and government -- 1992- , Ethnicity -- Mauritius
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2124 , http://hdl.handle.net/10962/d1019999
- Description: This thesis examines young people’s constructions of nationhood in Mauritius. In 2008, the Mauritian government instituted a Truth and Justice Commission (TJC), set up to investigate the consequences of slavery and indentured labour. Through the Truth and Justice Commission, the Mauritian government indicated its desire to achieve social justice and national unity. Drawing on developments in studies of national identification practices in the 21st Century, this thesis addresses the question of young Mauritian’s locally and globally informed identification practices and asks how their unofficial narratives of nationhood challenge, or divert, or relate to official state narratives of nationhood. The basis of the study emerges from data collected from 132 participants during fieldwork in multiple fieldsites from May to September 2010 as well as research on Mauritian youth on-line from 2011-2014. The advent of the TJC offers an ideal moment to evaluate the dynamics of post-colonial nation-building and nationhood in a selfstyled multi-cultural state. Nationhood, does not exist apriori to the constructions of narratives of the nation, thus the stories told about the nation, imagine the nation into being. By situating the Truth and Justice Commission and other official state narratives alongside young people’s narratives, I argue that contemporary narratives of nationhood in Mauritius represent an intergenerational struggle to define the meaning of the past in the present and consequently outline the future. Reflecting on the ideas and socio-economic and political processes that induce national consciousness, I argue that young people’s narratives of everyday lived experiences are vital for an interpretation of how nationhood is produced in everyday life. The cultural projects of young people – often rendered as liminal or marginal – offer a critical vantage point from where to read constructions of nationhood. Far from being growing pains or childish games, young people’s identity making practices are what Sherry B. Ortner has called “serious games.” This research suggests that official state government narratives of multicultural nationhood in Mauritius narrowly define national identification along communal loyalties, overlooking the dynamism of interculturality and transnationalism in daily practice on the island. Although communalism and rigid colonial interpretations of ethnicity attempt to police and limit the possibilities of alternative modes of being in Mauritius, young people’s identification practices question, challenge, and threaten to disrupt official discourses of ethnic identification in Mauritius Scholarly investigations of young peoples’ lived experiences of nationhood extend theoretical and methodological frames for the study of nationalized subjects and deepen the understanding of the construction of national consciousness. The construction of nationhood always involves narratives of some sort – scholarship on this area has usually focused on official state narratives from social theorists, state governments, and state elites. I argue for the importance of considering subjectivity and lived experience in conceptions of nationhood. In contemporary post-colonial societies, young people are the numerical majority, however, their voices are seldom represented in theories and narratives of nationhood. Whilst young people may appear in state policies (especially education) and official narratives about the future of the nation, their creative imagining and reimagining of narratives of selfhood is often ignored. I examine how young people increasingly are aware of their transnational connections, through participation in transnational youth cultures, and they are consequently increasingly multi-lingual and multicultural. Fixed notions of ethnic identification and discourses of trauma are not at the forefront of young people’s identification of selfhood, rather their ability to take advantage of their multiply situated identification processes allows them new means to evade and transform these narratives. Their identification of selfhood is characterised by a greater degree of dynamism than previous generations had access to, and thus they do not only identify themselves through officially sanctioned national forms of identification. Loyalty to nationhood is thus less predictable, and young people represent a potential threat to the continuation of older forms of nationhood. While official narratives of nationhood may manipulate ethnic and racial cleavages to secure old loyalties, not all young people are persuaded by these notions
- Full Text:
Reading identities: a case study of grade 8 learners' interactions in a reading club
- Scheckle, Eileen Margaret Agnes
- Authors: Scheckle, Eileen Margaret Agnes
- Date: 2015
- Subjects: Group reading -- South Africa , Reading (Middle school) -- South Africa , Literacy programs -- South Africa , Identity (Psychology) in adolescence , Identity (Psychology) in adolescence -- South Africa , Discourse analysis -- Social aspects , Critical realism
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1329 , http://hdl.handle.net/10962/d1017766
- Description: This study offers an account of reading clubs as a literacy intervention in a grade 8 English class at a former ‘Coloured’ high school in South Africa. Using Margaret Archer’s social realist methodology, it examines different practices of ‘reading’ used by learners in talking and writing about text. Archer’s analytical dualism and morphogenetic model provided an explanatory framework for this study. Analytical dualism allows for the separation of the parts (structural and cultural elements) from the people (the grade 8 learners) so as to analyse the interplay between structure and culture. The morphogenetic model recognises that antecedent structures predate this, and any study but that through the exercise of agency, morphogenesis, in the form of structural elaboration or morphostasis in the form of continuity, may occur. This study used a New Literacies perspective based on an ideological model of literacy which recognises many different literacies, in addition to dominant school literacies. Learners’ talk about books as well as personal journal writing provided an insight into what cultural mechanisms and powers children bring to the reading of novels. Understandings of discourses as well as of Gee’s (1990; 2008) construct of Discourse provided a framework for examining learners’ identities and shifts as readers. The data in this study, which is presented through a series of vignettes, found that grade 8 learners use many different experiences and draw on different discourses when making sense of texts. Through the separation of the structural and cultural components, this research could explore how reading clubs as structures enabled learners to access different discourses from the domain of culture. Through the process and engagement in the reading clubs, following Gee (2000b), learners were attributed affinity, discoursal and institutional identities as readers. It was found, in the course of the study, that providing a safe space, scaffolding, multiple opportunities to practice and a variety of reading material, helped learners to access and appropriate dominant literacies. In addition, learners need a repertoire of literacy practices to draw from as successful reading needs flexibility and adaptability. Reading and writing inform each other and through gradual induction into literary writing, learners began to appropriate and approximate dominant literacy practices. Following others who have contributed to the field of New Literacy Studies (Heath, 1983; Street, 1984; Gee 1990; Prinsloo & Breier, 1996), this study would suggest that literacies of traditionally underserved communities should not be considered in deficit terms. Instead these need to be understood as resources for negotiating meaning making and as tools or mechanisms to access dominant discourse practices. In addition the resilience and competition from Discourses of popular culture need to be recognised and developed as tools to access school literacies.
- Full Text:
- Authors: Scheckle, Eileen Margaret Agnes
- Date: 2015
- Subjects: Group reading -- South Africa , Reading (Middle school) -- South Africa , Literacy programs -- South Africa , Identity (Psychology) in adolescence , Identity (Psychology) in adolescence -- South Africa , Discourse analysis -- Social aspects , Critical realism
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1329 , http://hdl.handle.net/10962/d1017766
- Description: This study offers an account of reading clubs as a literacy intervention in a grade 8 English class at a former ‘Coloured’ high school in South Africa. Using Margaret Archer’s social realist methodology, it examines different practices of ‘reading’ used by learners in talking and writing about text. Archer’s analytical dualism and morphogenetic model provided an explanatory framework for this study. Analytical dualism allows for the separation of the parts (structural and cultural elements) from the people (the grade 8 learners) so as to analyse the interplay between structure and culture. The morphogenetic model recognises that antecedent structures predate this, and any study but that through the exercise of agency, morphogenesis, in the form of structural elaboration or morphostasis in the form of continuity, may occur. This study used a New Literacies perspective based on an ideological model of literacy which recognises many different literacies, in addition to dominant school literacies. Learners’ talk about books as well as personal journal writing provided an insight into what cultural mechanisms and powers children bring to the reading of novels. Understandings of discourses as well as of Gee’s (1990; 2008) construct of Discourse provided a framework for examining learners’ identities and shifts as readers. The data in this study, which is presented through a series of vignettes, found that grade 8 learners use many different experiences and draw on different discourses when making sense of texts. Through the separation of the structural and cultural components, this research could explore how reading clubs as structures enabled learners to access different discourses from the domain of culture. Through the process and engagement in the reading clubs, following Gee (2000b), learners were attributed affinity, discoursal and institutional identities as readers. It was found, in the course of the study, that providing a safe space, scaffolding, multiple opportunities to practice and a variety of reading material, helped learners to access and appropriate dominant literacies. In addition, learners need a repertoire of literacy practices to draw from as successful reading needs flexibility and adaptability. Reading and writing inform each other and through gradual induction into literary writing, learners began to appropriate and approximate dominant literacy practices. Following others who have contributed to the field of New Literacy Studies (Heath, 1983; Street, 1984; Gee 1990; Prinsloo & Breier, 1996), this study would suggest that literacies of traditionally underserved communities should not be considered in deficit terms. Instead these need to be understood as resources for negotiating meaning making and as tools or mechanisms to access dominant discourse practices. In addition the resilience and competition from Discourses of popular culture need to be recognised and developed as tools to access school literacies.
- Full Text:
Saving the Sowetan : the public interest and commercial imperatives in journalism practice
- Authors: Cowling, Lesley
- Date: 2015
- Subjects: Sowetan (Johannesburg, South Africa) , Journalism -- Economic aspects -- South Africa -- Soweto , Journalism -- Political aspects -- South Africa -- Soweto , Black newspapers -- South Africa -- Soweto , Public interest -- South Africa -- Soweto , Corporate culture -- South Africa -- Soweto , Journalistic ethics -- South Africa -- Soweto , Journalism -- Objectivity -- South Africa -- Soweto
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3540 , http://hdl.handle.net/10962/d1017781
- Description: This thesis examines the complex ways in which notions of the public interest and commercial imperatives intertwine in journalism practice. It does this through a study of the 2004 takeover and relaunch of the Sowetan newspaper, the highest circulation daily in South Africa throughout the 1990s and an institution of black public life. The ‘public interest’ and ‘the commercial’ are recurring ideas in journalism scholarship and practice, and the relaunch appeared to be a challenge to reconcile the Sowetan’s commercial challenges with its historical responsibility to a ‘nation-building’ public. However, the research shows that the public/commercial aspects of journalism were inextricably entangled with Sowetan’s organisational culture, which was the matrix through which its journalism practice was expressed. Conflict in the organisation over the changes was not simply a contest between commercial realities and the public interest, with journalists defending a responsibility to the public and managers pushing commercial solutions, but a conflict between the culture of Sowetan “insiders”, steeped in the legacy of the newspaper, and “outsiders”, employed by the new owners to effect change. Another conclusion of the research is that commercial “realities” – often conceptualised as counter to the public interest – are highly mutable. Basic conditions, such as a dependence on advertising, exist. However, media managers must choose from a range of strategies to be commercially viable, which requires risk-taking, innovation and, often, guesswork. In such situations, the ‘wall’ between media managers and senior editors is porous, as all executives must manage the relationship between business and editorial imperatives. Executives tend to overlook culture as a factor in changing organisations, but I argue that journalism could benefit from engaging with management theory and organisational psychology, which offer ways to understand the specific dynamics of the organisation. Finally, I argue that the case of the Sowetan throws into question the idea that there may be a broadly universal journalism culture. The attachment of Sowetan journalists to their particular values and practice suggests that forms of journalism evolve in certain contexts to diverge from the ‘professional’ Anglo-American modes. These ‘journalisms’ use similar terms – such as the ‘public interest’ – but operationalise them quite differently. The responsibility to the public is imagined in very different ways, but remains a significant attachment for journalists.
- Full Text:
- Authors: Cowling, Lesley
- Date: 2015
- Subjects: Sowetan (Johannesburg, South Africa) , Journalism -- Economic aspects -- South Africa -- Soweto , Journalism -- Political aspects -- South Africa -- Soweto , Black newspapers -- South Africa -- Soweto , Public interest -- South Africa -- Soweto , Corporate culture -- South Africa -- Soweto , Journalistic ethics -- South Africa -- Soweto , Journalism -- Objectivity -- South Africa -- Soweto
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3540 , http://hdl.handle.net/10962/d1017781
- Description: This thesis examines the complex ways in which notions of the public interest and commercial imperatives intertwine in journalism practice. It does this through a study of the 2004 takeover and relaunch of the Sowetan newspaper, the highest circulation daily in South Africa throughout the 1990s and an institution of black public life. The ‘public interest’ and ‘the commercial’ are recurring ideas in journalism scholarship and practice, and the relaunch appeared to be a challenge to reconcile the Sowetan’s commercial challenges with its historical responsibility to a ‘nation-building’ public. However, the research shows that the public/commercial aspects of journalism were inextricably entangled with Sowetan’s organisational culture, which was the matrix through which its journalism practice was expressed. Conflict in the organisation over the changes was not simply a contest between commercial realities and the public interest, with journalists defending a responsibility to the public and managers pushing commercial solutions, but a conflict between the culture of Sowetan “insiders”, steeped in the legacy of the newspaper, and “outsiders”, employed by the new owners to effect change. Another conclusion of the research is that commercial “realities” – often conceptualised as counter to the public interest – are highly mutable. Basic conditions, such as a dependence on advertising, exist. However, media managers must choose from a range of strategies to be commercially viable, which requires risk-taking, innovation and, often, guesswork. In such situations, the ‘wall’ between media managers and senior editors is porous, as all executives must manage the relationship between business and editorial imperatives. Executives tend to overlook culture as a factor in changing organisations, but I argue that journalism could benefit from engaging with management theory and organisational psychology, which offer ways to understand the specific dynamics of the organisation. Finally, I argue that the case of the Sowetan throws into question the idea that there may be a broadly universal journalism culture. The attachment of Sowetan journalists to their particular values and practice suggests that forms of journalism evolve in certain contexts to diverge from the ‘professional’ Anglo-American modes. These ‘journalisms’ use similar terms – such as the ‘public interest’ – but operationalise them quite differently. The responsibility to the public is imagined in very different ways, but remains a significant attachment for journalists.
- Full Text:
Sediment connectivity in the upper Thina Catchment, Eastern Cape, South Africa
- Van der Waal, Benjamin Cornelis Wentsel
- Authors: Van der Waal, Benjamin Cornelis Wentsel
- Date: 2015
- Subjects: Soil erosion -- South Africa -- Eastern Cape , Sedimentation and deposition -- South Africa -- Eastern Cape , Watersheds -- South Africa -- Eastern Cape , Arroyos -- South Africa -- Eastern Cape , Sediment transport -- South Africa -- Eastern Cape , Soil conservation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4892 , http://hdl.handle.net/10962/d1019754
- Description: [Portion of abstract]: Sediment dynamics are influenced by transformed landscape connectivity in catchments worldwide. The upper Thina catchment, an important high rainfall resource in the northern Eastern Cape, South Africa, is an example of where ongoing subsistence farming on communal land has led to overgrazing and trampling that has initiated large erosive features (e.g. gullies) and river incision. The formation of gullies led to increased hillslope-channel connectivity and the resultant river incision decreased the channel-valley fill connectivity. These two changes in connectivity led to increased sediment export from the catchment that has various down-stream ecological and socio-economic impacts. This study investigates how the change in hillslope-channel and channel-valley fill connectivity has altered the sediment dynamics in the Vuvu catchment, a headwater tributary of the Thina River. A combination of methods were used to assess the changes in hillslope-channel and channel-valley fill connectivity. High resolution aerial images were used to map source features, such as fields, gullies, sheet erosion, landslides, roads and livestock tracks. Topographic and geological characteristics of the source features were extracted using a Geographic Information System. Furthermore, hillslope-channel pathways, such as the natural drainage network, continuous gullies, discontinuous gullies, roads and livestock tracks were mapped and analysed in terms of topographic and geological characteristics. Historic aerial images were assessed to calculate the date the larger gullies began forming. Recent aerial photos and cross sectional surveys of the valley fill were combined to map the various sediment sinks. Particle size and organic content were analysed for flood bench cores and terrace samples. The chronology of the flood benches was determined using unsupported Pb-210 and Cs-137 dating, and determined for the terraces using Optically Stimulated Luminescence dating. Quantitative and qualitative sediment tracing approaches, using mineral magnetic properties, were used to trace the origin of suspended sediment (collected during flood events), sediment stored in the flood benches and sediment stored in the terraces. Hydrological monitoring was used to assess the potential to store sediment on flood benches along the valley fill through flood bench inundation frequency. Hydrological and hydraulic modelling extended the measured inundation frequencies to a 73 year period and other cross sections along the valley fill. Furthermore, a future scenario of an increased vegetation cover and reduced hillslope-channel connectivity was assessed in terms of channel-valley fill inundation frequency.
- Full Text:
- Authors: Van der Waal, Benjamin Cornelis Wentsel
- Date: 2015
- Subjects: Soil erosion -- South Africa -- Eastern Cape , Sedimentation and deposition -- South Africa -- Eastern Cape , Watersheds -- South Africa -- Eastern Cape , Arroyos -- South Africa -- Eastern Cape , Sediment transport -- South Africa -- Eastern Cape , Soil conservation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4892 , http://hdl.handle.net/10962/d1019754
- Description: [Portion of abstract]: Sediment dynamics are influenced by transformed landscape connectivity in catchments worldwide. The upper Thina catchment, an important high rainfall resource in the northern Eastern Cape, South Africa, is an example of where ongoing subsistence farming on communal land has led to overgrazing and trampling that has initiated large erosive features (e.g. gullies) and river incision. The formation of gullies led to increased hillslope-channel connectivity and the resultant river incision decreased the channel-valley fill connectivity. These two changes in connectivity led to increased sediment export from the catchment that has various down-stream ecological and socio-economic impacts. This study investigates how the change in hillslope-channel and channel-valley fill connectivity has altered the sediment dynamics in the Vuvu catchment, a headwater tributary of the Thina River. A combination of methods were used to assess the changes in hillslope-channel and channel-valley fill connectivity. High resolution aerial images were used to map source features, such as fields, gullies, sheet erosion, landslides, roads and livestock tracks. Topographic and geological characteristics of the source features were extracted using a Geographic Information System. Furthermore, hillslope-channel pathways, such as the natural drainage network, continuous gullies, discontinuous gullies, roads and livestock tracks were mapped and analysed in terms of topographic and geological characteristics. Historic aerial images were assessed to calculate the date the larger gullies began forming. Recent aerial photos and cross sectional surveys of the valley fill were combined to map the various sediment sinks. Particle size and organic content were analysed for flood bench cores and terrace samples. The chronology of the flood benches was determined using unsupported Pb-210 and Cs-137 dating, and determined for the terraces using Optically Stimulated Luminescence dating. Quantitative and qualitative sediment tracing approaches, using mineral magnetic properties, were used to trace the origin of suspended sediment (collected during flood events), sediment stored in the flood benches and sediment stored in the terraces. Hydrological monitoring was used to assess the potential to store sediment on flood benches along the valley fill through flood bench inundation frequency. Hydrological and hydraulic modelling extended the measured inundation frequencies to a 73 year period and other cross sections along the valley fill. Furthermore, a future scenario of an increased vegetation cover and reduced hillslope-channel connectivity was assessed in terms of channel-valley fill inundation frequency.
- Full Text:
Social-ecological resilience for well-being : a critical realist case study of Boksburg Lake, South Africa
- Authors: Fox, Helen Elizabeth
- Date: 2015
- Subjects: Boksburg Lake and Wetland project , Reservoirs -- South Africa -- Boksburg , Water -- Pollution -- South Africa -- Boksburg , Human ecology -- South Africa -- Boksburg , Social learning -- South Africa -- Boksburg , Critical realism
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6048 , http://hdl.handle.net/10962/d1017872
- Description: This thesis is based on a case study of the degraded Boksburg Lake social-ecological system and an environmental education initiative that aimed to support its transformation. This initiative aimed to involve local people in reclaiming the lake’s social and ecological value, through a process of collectively reimagining possibilities, shaping identities, gaining knowledge and developing local human agency. The focus was on social learning processes in schools and churches to explore opportunities for co-engaged reflexivity that might produce transformation. Schools and Christian churches, two institutions that reflect modern, western socialecological worldviews also have the potential to bring about change. Critical Realism was chosen as my philosophical framework as it provided the tools to explore deeper mechanisms beyond empirical reality, both influencing the degrading trajectory as well as providing possibilities for transformation. It also legitimised case study research as a means to understand more generalised processes characterising modern social-ecological systems. The choice of Critical Realism informed the scope of my primary research question: What generative mechanisms constrain and enable the development of social-ecological resilience for well-being, in the modern social-ecological system of Boksburg Lake? The following three goals were formulated to address this primary question. Goal 1: Based on a multitheoretical perspective of social-ecological literature, develop conceptual tools that have explanatory power to probe generative mechanisms operating in the Boksburg Lake social-ecological system. Goal 2: Identify generative mechanisms driving the current degradation of the Boksburg Lake social-ecological system. Goal 3: Identify learning mechanisms that support transformation for greater social-ecological resilience of the Boksburg Lake social-ecological system. By addressing the primary question and research goals I aimed to gain insights into modern global socialecological systems, the mechanisms that drive high social-ecological risk and the requirements for and possibilities of global systemic change. Drawing on a broad reading of social-ecological literature from different vantage points, tools with explanatory power were developed to probe for generative mechanisms in the Boksburg Lake social-ecological system (goal 1). The human capacity for symbolic representation is identified as an emergent property of coevolving human-ecological systems. These symbolic representations become expressed in culture and worldviews, and influence patterns of identifying, types of knowledge and forms of agency. The nature of these will determine the degree that cultural systems are embedded within ecological reality and the extent of cultural-ecological coupling. A cultural system closely coupled with ecological realities is likely to value ecological systems and manage them for their health, while less coupled cultural-ecological systems are likely to lead to the opposite. Because of their integrated nature, the extent of ecological health and value will affect the decline or sustainability of cultural-ecological systems. There are numerous examples of the learning that can take place when cultural-ecological systems are facing decline. This learning can enhance or reduce biophyllic instincts that become encoded in patterns of identifying, types of knowledge and forms of agency. This in turn affects the strength of cultural-ecological coupling and the extent that human societies co-evolve with ecological systems. , Normalising ideologies is a concept coined in the thesis to refer to symbolic representations of reality that have become integral to a social fabric and determine meaning, while maintaining the domination of the powerful. These ideologies determine patterns of identifying, knowledge and agency and are recognised as having a fundamental influence on the resilience of social-ecological systems. Four normalising ideologies are identified that promote apparent human progress at the expense of ecological integrity and social equality and thus alienation with each other and the ecological world. These are human-ecological dualism, anthropocentrism, nature is mechanised and nature is to be controlled. There are also a number of ideologies promoting connectedness with the ecological world that, if they became normalised, would support greater social-ecological resilience for well-being. Generative mechanisms driving the current degradation of the Boksburg Lake socialecological system were identified (goal 2). Drawing on critical methodology, the main method adopted was document analysis of the Boksburg Advertiser archives, Boksburg’s local newspaper. Four generative mechanisms are recognised as most influential. Two of these have been named hegemonic symbolic systems. The primary symbolic system consists of the four normalising ideologies, mentioned above, that promote human progress at the expense of ecological health. The secondary, more explicit symbolic system, built on this, consists of the following normative ideologies: economic growth is imperative, unrestrained development is promoted, competition is the necessary means and consumerism is the good life. These two symbolic systems have had causal influence on the systematic erosion of ecological processes and biological diversity that has occurred in Boksburg, with the consequent undermining of social-ecological resilience for well-being. The third mechanism that constrains resilience is the power dynamics that have shaped Boksburg’s economic history and social-ecological system. This has resulted in a society built on inequality and injustice with all its associated social and environmental ills, expressed as externalities. The fourth mechanism resides in Boksburg’s political and municipal dynamics. These structures are not designed to tackle complex social-ecological problems and they hold considerable agential power, yet seem dysfunctional at present. Learning mechanisms that support transformation for greater social-ecological resilience of the Boksburg Lake social-ecological system were identified (goal 3). By adopting the role of a reflexive practitioner, supported by action research, case study and interpretivist methodologies, data on the empirical manifestations of the environmental educational initiative were collected. Methods included semistructured interviews, focus groups, document analysis and participant observation. Findings indicate that schools and churches are important institutions that can positively influence patterns of identifying, knowledge about and agency for Boksburg Lake and can thus play a role in transforming hegemonic normalising ideologies. Important learning mechanisms identified included: Learning reflexively together within communities of practice that provide opportunities for active rather than passive learning; involving the youth as they are a group of people with notable enthusiasm, vision, energy and motivation; learning through information acquisition, investigation, action and deliberation; learning about abstract concepts and theoretical knowledge but embedding this in local realities; and learning that provides reference markers for how things can be different.
- Full Text:
- Authors: Fox, Helen Elizabeth
- Date: 2015
- Subjects: Boksburg Lake and Wetland project , Reservoirs -- South Africa -- Boksburg , Water -- Pollution -- South Africa -- Boksburg , Human ecology -- South Africa -- Boksburg , Social learning -- South Africa -- Boksburg , Critical realism
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6048 , http://hdl.handle.net/10962/d1017872
- Description: This thesis is based on a case study of the degraded Boksburg Lake social-ecological system and an environmental education initiative that aimed to support its transformation. This initiative aimed to involve local people in reclaiming the lake’s social and ecological value, through a process of collectively reimagining possibilities, shaping identities, gaining knowledge and developing local human agency. The focus was on social learning processes in schools and churches to explore opportunities for co-engaged reflexivity that might produce transformation. Schools and Christian churches, two institutions that reflect modern, western socialecological worldviews also have the potential to bring about change. Critical Realism was chosen as my philosophical framework as it provided the tools to explore deeper mechanisms beyond empirical reality, both influencing the degrading trajectory as well as providing possibilities for transformation. It also legitimised case study research as a means to understand more generalised processes characterising modern social-ecological systems. The choice of Critical Realism informed the scope of my primary research question: What generative mechanisms constrain and enable the development of social-ecological resilience for well-being, in the modern social-ecological system of Boksburg Lake? The following three goals were formulated to address this primary question. Goal 1: Based on a multitheoretical perspective of social-ecological literature, develop conceptual tools that have explanatory power to probe generative mechanisms operating in the Boksburg Lake social-ecological system. Goal 2: Identify generative mechanisms driving the current degradation of the Boksburg Lake social-ecological system. Goal 3: Identify learning mechanisms that support transformation for greater social-ecological resilience of the Boksburg Lake social-ecological system. By addressing the primary question and research goals I aimed to gain insights into modern global socialecological systems, the mechanisms that drive high social-ecological risk and the requirements for and possibilities of global systemic change. Drawing on a broad reading of social-ecological literature from different vantage points, tools with explanatory power were developed to probe for generative mechanisms in the Boksburg Lake social-ecological system (goal 1). The human capacity for symbolic representation is identified as an emergent property of coevolving human-ecological systems. These symbolic representations become expressed in culture and worldviews, and influence patterns of identifying, types of knowledge and forms of agency. The nature of these will determine the degree that cultural systems are embedded within ecological reality and the extent of cultural-ecological coupling. A cultural system closely coupled with ecological realities is likely to value ecological systems and manage them for their health, while less coupled cultural-ecological systems are likely to lead to the opposite. Because of their integrated nature, the extent of ecological health and value will affect the decline or sustainability of cultural-ecological systems. There are numerous examples of the learning that can take place when cultural-ecological systems are facing decline. This learning can enhance or reduce biophyllic instincts that become encoded in patterns of identifying, types of knowledge and forms of agency. This in turn affects the strength of cultural-ecological coupling and the extent that human societies co-evolve with ecological systems. , Normalising ideologies is a concept coined in the thesis to refer to symbolic representations of reality that have become integral to a social fabric and determine meaning, while maintaining the domination of the powerful. These ideologies determine patterns of identifying, knowledge and agency and are recognised as having a fundamental influence on the resilience of social-ecological systems. Four normalising ideologies are identified that promote apparent human progress at the expense of ecological integrity and social equality and thus alienation with each other and the ecological world. These are human-ecological dualism, anthropocentrism, nature is mechanised and nature is to be controlled. There are also a number of ideologies promoting connectedness with the ecological world that, if they became normalised, would support greater social-ecological resilience for well-being. Generative mechanisms driving the current degradation of the Boksburg Lake socialecological system were identified (goal 2). Drawing on critical methodology, the main method adopted was document analysis of the Boksburg Advertiser archives, Boksburg’s local newspaper. Four generative mechanisms are recognised as most influential. Two of these have been named hegemonic symbolic systems. The primary symbolic system consists of the four normalising ideologies, mentioned above, that promote human progress at the expense of ecological health. The secondary, more explicit symbolic system, built on this, consists of the following normative ideologies: economic growth is imperative, unrestrained development is promoted, competition is the necessary means and consumerism is the good life. These two symbolic systems have had causal influence on the systematic erosion of ecological processes and biological diversity that has occurred in Boksburg, with the consequent undermining of social-ecological resilience for well-being. The third mechanism that constrains resilience is the power dynamics that have shaped Boksburg’s economic history and social-ecological system. This has resulted in a society built on inequality and injustice with all its associated social and environmental ills, expressed as externalities. The fourth mechanism resides in Boksburg’s political and municipal dynamics. These structures are not designed to tackle complex social-ecological problems and they hold considerable agential power, yet seem dysfunctional at present. Learning mechanisms that support transformation for greater social-ecological resilience of the Boksburg Lake social-ecological system were identified (goal 3). By adopting the role of a reflexive practitioner, supported by action research, case study and interpretivist methodologies, data on the empirical manifestations of the environmental educational initiative were collected. Methods included semistructured interviews, focus groups, document analysis and participant observation. Findings indicate that schools and churches are important institutions that can positively influence patterns of identifying, knowledge about and agency for Boksburg Lake and can thus play a role in transforming hegemonic normalising ideologies. Important learning mechanisms identified included: Learning reflexively together within communities of practice that provide opportunities for active rather than passive learning; involving the youth as they are a group of people with notable enthusiasm, vision, energy and motivation; learning through information acquisition, investigation, action and deliberation; learning about abstract concepts and theoretical knowledge but embedding this in local realities; and learning that provides reference markers for how things can be different.
- Full Text:
Spatial and temporal variations in trophic connectivity within an estuarine environment : benthic-pelagic and terrestrial-aquatic linkages via invertebrates and fishes
- Authors: Bergamino Roman, Leandro
- Date: 2015
- Subjects: Estuarine ecology -- South Africa , Marine invertebrates -- South Africa -- Ecology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5921 , http://hdl.handle.net/10962/d1017799
- Description: Estuarine ecosystems are among the most biologically productive areas and they provide important ecosystem services such as erosion control, habitat and refugia for several species. These environments are characterized by the presence of a variety of organic matter sources due to their transitional position between rivers and the sea. The biotic compositions can undergo spatial and seasonal changes along the estuary due to the spatial and temporal fluctuations of environmental factors such as salinity, temperature and seston loads. Therefore, the different combinations of biotic and abiotic factors make each estuary a unique ecosystem. Because of this spatial and temporal complexity, the understanding of estuarine food web structure and which factors affect the trophic relationships within the ecosystem through space and time represent challenging tasks. Furthermore, estuaries are under an increasing number of anthropogenic perturbations because of the growing concentration of human populations in coastal areas. Knowledge of ecosystem structure and functioning is essential for effective conservation and management planning of coastal areas.In this dissertation, I combine the utilization of biological tracers to examine spatial and temporal variability in the food web structure within a small temperate and microtidal estuary located in South Africa. To this end, fatty acid profiles and stable isotope signatures were measured in several primary organic matter sources and consumers (including zooplankton, fishes and benthic invertebrates) during four consecutive seasons and in three different estuarine regions: upper, middle, and lower reaches. The three reaches had distinct habitat features of vegetation type and morphology, and in particular the lower reaches were colonized by the marsh grass Spartina maritima. Isotopic mixing models were used to estimate the relative contribution of each food source to the diets of invertebrates and fishes within the estuarine food web. The isotopic and fatty acid data showed similar results. In general, the lower reaches of the estuary were characterized by a higher deposition and assimilation by brachyuran crabs of carbon derived from marsh grass detritus, whiletowards the upper reaches a mixture of microphytobenthos and particulate organic matter (phytoplankton and detritus) was deposited and sustained the pelagic and benthic fauna. The highest deposition and assimilation of marsh grass detritus in the lower reaches of the estuary occurred during periods of low freshwater discharge (autumn and winter). In the upper reaches, microphytobenthos and suspended particulate organic matter were dominant basal food resources for the food web during all seasons. These results indicated that benthic consumers incorporated mainly local carbon sources from their local habitat.To clarify isotopic and fatty acid patterns I examined the trophic behaviour of the sesarmid crab Sesarma catenata through laboratory feeding experiments. Results from these experiments validated that decomposed leaves of riparian trees and the salt marsh plant S. maritima were the preferred food of the sesarmid crabs, potentially due to high bacterial loads. The remaining leaf material not assimilated by crabs, together with faecal material, are likely important subsidies for adjacent environments, hence representing an important energy pathway involving the microbial food chain. Furthermore, this dissertation showed the importance of mobile top predators as vectors energetically connecting distinct food chains within the estuary (i.e. littoral, benthic and pelagic). I concluded that a combination of physical (i.e. patterns of freshwater discharge and estuary morphology) and biological factors (i.e. organism feeding behaviour, mobility, primary productivity, the local vegetation type) influence the pattern of dominant primary organic matter sources, and therefore the food web structure along the estuarine environment. In particular, marsh grass detritus contributed substantially to the diets of estuarine fauna during periods of low freshwater discharge. Given the importance of the salt marsh habitat in providing trophic resources, it is important to preserve this environment to sustain the natural biota and ecosystem functioning.
- Full Text:
- Authors: Bergamino Roman, Leandro
- Date: 2015
- Subjects: Estuarine ecology -- South Africa , Marine invertebrates -- South Africa -- Ecology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5921 , http://hdl.handle.net/10962/d1017799
- Description: Estuarine ecosystems are among the most biologically productive areas and they provide important ecosystem services such as erosion control, habitat and refugia for several species. These environments are characterized by the presence of a variety of organic matter sources due to their transitional position between rivers and the sea. The biotic compositions can undergo spatial and seasonal changes along the estuary due to the spatial and temporal fluctuations of environmental factors such as salinity, temperature and seston loads. Therefore, the different combinations of biotic and abiotic factors make each estuary a unique ecosystem. Because of this spatial and temporal complexity, the understanding of estuarine food web structure and which factors affect the trophic relationships within the ecosystem through space and time represent challenging tasks. Furthermore, estuaries are under an increasing number of anthropogenic perturbations because of the growing concentration of human populations in coastal areas. Knowledge of ecosystem structure and functioning is essential for effective conservation and management planning of coastal areas.In this dissertation, I combine the utilization of biological tracers to examine spatial and temporal variability in the food web structure within a small temperate and microtidal estuary located in South Africa. To this end, fatty acid profiles and stable isotope signatures were measured in several primary organic matter sources and consumers (including zooplankton, fishes and benthic invertebrates) during four consecutive seasons and in three different estuarine regions: upper, middle, and lower reaches. The three reaches had distinct habitat features of vegetation type and morphology, and in particular the lower reaches were colonized by the marsh grass Spartina maritima. Isotopic mixing models were used to estimate the relative contribution of each food source to the diets of invertebrates and fishes within the estuarine food web. The isotopic and fatty acid data showed similar results. In general, the lower reaches of the estuary were characterized by a higher deposition and assimilation by brachyuran crabs of carbon derived from marsh grass detritus, whiletowards the upper reaches a mixture of microphytobenthos and particulate organic matter (phytoplankton and detritus) was deposited and sustained the pelagic and benthic fauna. The highest deposition and assimilation of marsh grass detritus in the lower reaches of the estuary occurred during periods of low freshwater discharge (autumn and winter). In the upper reaches, microphytobenthos and suspended particulate organic matter were dominant basal food resources for the food web during all seasons. These results indicated that benthic consumers incorporated mainly local carbon sources from their local habitat.To clarify isotopic and fatty acid patterns I examined the trophic behaviour of the sesarmid crab Sesarma catenata through laboratory feeding experiments. Results from these experiments validated that decomposed leaves of riparian trees and the salt marsh plant S. maritima were the preferred food of the sesarmid crabs, potentially due to high bacterial loads. The remaining leaf material not assimilated by crabs, together with faecal material, are likely important subsidies for adjacent environments, hence representing an important energy pathway involving the microbial food chain. Furthermore, this dissertation showed the importance of mobile top predators as vectors energetically connecting distinct food chains within the estuary (i.e. littoral, benthic and pelagic). I concluded that a combination of physical (i.e. patterns of freshwater discharge and estuary morphology) and biological factors (i.e. organism feeding behaviour, mobility, primary productivity, the local vegetation type) influence the pattern of dominant primary organic matter sources, and therefore the food web structure along the estuarine environment. In particular, marsh grass detritus contributed substantially to the diets of estuarine fauna during periods of low freshwater discharge. Given the importance of the salt marsh habitat in providing trophic resources, it is important to preserve this environment to sustain the natural biota and ecosystem functioning.
- Full Text:
Spatio-temporal variation in the phytobenthos and phytoplankton community structure and composition of particulate matter along a river-estuary continuum assessed using microscopic and stable isotope analyses
- Authors: Dalu, Tatenda
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54416 , vital:26563
- Description: Phytoplankton and phytobenthos communities play an important role in lotic systems as primary producers providing essential biomolecules to higher trophic oganisms and are important indicators for environmental or ecological change. In this thesis, field studies (observational and experimental) along a river–estuary continuum were conducted to assess the spatio-temporal variation and development of phytobenthos and phytoplankton communities using a combination of stable isotope and community analyses in a temperate southern African system across four study periods: September (early spring) and November/December (late spring) 2012, and February (summer) and May/June (winter) 2013. Additionally, the sources and composition of the particulate organic matter were also analysed using stable isotope (δ15N and δ13C) analysis. The effects of substrate type and flood occurrence were assessed through experimental studies at an up- and downstream site of the river after a major flood event that occurred between October and November 2012. Common household tiles were used as artificial substrates to study the development/succession of phytobenthos communities after the flood disturbance. Distinct diatom communities were observed between upstream and downstream sites and at each site, community structure changed with time indicating succession. In addition to recording diatom characteristics on three natural substrates, namely; macrophytes, rocks and sediment, artificial substrates observations were also made on three different types of artificial substrates, namely; brick, brown clay and grey clay tiles. The natural (species richness 78) and artificial substrates (sp. richness 93) had different communities with the latter having greater species richness. Common phytobenthos taxa were not restricted to a single substrate but preference was generally high for the artificial substrates, especially brown tiles (mean sp. richness 47). Results of the redundancy analysis (RDA) analysis indicated that ammonium, conductivity, total dissolved solids, salinity, pH, oxygen reduction potential, phosphate and water depth were the major determinants of the phytobenthos composition at the two sites. The spatio–temporal variation of phytoplankton and phytobenthos communities and allochthonous organic matter along the river–estuary continuum was assessed at 8 sites using a combination of community and stable isotope analyses. A total of 178 species belonging to 78 genera were recorded with diatoms being predominant, accounting for 81.9 % of the total abundance. The total chl-a concentration along the river-estuary continuum increased from spring to a high in summer before decreasing to a low in winter. Periphyton communities were observed to be significantly different across sites (p < 0.05) in terms of species richness, abundances and isotopically The high periphytic δ15N values (range 7.9–15.2 ‰) recorded at the downstream sites compared to the pristine upstream sites (δ15N values range 4–7 ‰) suggest nutrient enrichment most likely derived from anthropogenic sources. Overall, our results reveal general patterns of periphyton communities and stable isotopes and provide improved information in the use of periphyton δ15N as an excellent indicator of anthropogenic nitrogen pollution. Ecologists are interested in the factors that control, and the variability in, the contributions of different sources to mixed organic materials traveling through lotic systems. We hypothesized that the source matter fuelling mixed organic pools in a river-estuary continuum varies over space and time, with the upper reaches of a system characterized by allochthonous-dominated material and autochthonous contributions becoming more important in the lower reaches. Samples of the mixed organic pools and allochthonous and autochthonous source materials were collected during the four study periods. The C:N ratios of suspended particulate matter (SPM) collected during summer and winter indicated that the lower reaches of the system had similar organic matter contributions from the freshwater and terrestrial sources. Stable isotope analysis in R revealed that the contributions of autochthonous organic matter were high in SPM along the entire continuum, and aquatic macrophytes were significant contributors to SPM specifically in the upper reaches. The terrestrial leaves made major contributions to the SPM in the middle regions of the system (i.e. downstream sites of the river, particularly in early and late spring). Bulk detritus had large allochthonous matter components in the lower reaches (estuary), and the contributions of aquatic macrophytes and benthic algae to bulk detritus were high (> 50 %) in the upper to middle reaches (river), but low (< 20 %) in the lower reaches (estuary). The current investigation represents the first attempt to assess the validity of the River Continuum Concept (RCC) in a southern African temperate river. The phytoplankton and phytobenthos communities, and chl-a concentration followed a trend similar to that proposed for the river continuum concept (RCC). The middle reaches based on the phytobenthos or phytoplankton communities and chl-a concentrations which were employed as proxies for primary production, were the most productive, while the upper reaches were the least primary productive. The evaluation of organic matter contributions to the SPM and detritus along the river–estuary continuum provided a baseline assessment of the nature and sources of potential food for consumers inhabiting different locations during different times of the year. Incorporating such spatio-temporal variations in SPM and detritus into food web studies will improve our understanding of the flow of carbon through aquatic systems.
- Full Text:
- Authors: Dalu, Tatenda
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54416 , vital:26563
- Description: Phytoplankton and phytobenthos communities play an important role in lotic systems as primary producers providing essential biomolecules to higher trophic oganisms and are important indicators for environmental or ecological change. In this thesis, field studies (observational and experimental) along a river–estuary continuum were conducted to assess the spatio-temporal variation and development of phytobenthos and phytoplankton communities using a combination of stable isotope and community analyses in a temperate southern African system across four study periods: September (early spring) and November/December (late spring) 2012, and February (summer) and May/June (winter) 2013. Additionally, the sources and composition of the particulate organic matter were also analysed using stable isotope (δ15N and δ13C) analysis. The effects of substrate type and flood occurrence were assessed through experimental studies at an up- and downstream site of the river after a major flood event that occurred between October and November 2012. Common household tiles were used as artificial substrates to study the development/succession of phytobenthos communities after the flood disturbance. Distinct diatom communities were observed between upstream and downstream sites and at each site, community structure changed with time indicating succession. In addition to recording diatom characteristics on three natural substrates, namely; macrophytes, rocks and sediment, artificial substrates observations were also made on three different types of artificial substrates, namely; brick, brown clay and grey clay tiles. The natural (species richness 78) and artificial substrates (sp. richness 93) had different communities with the latter having greater species richness. Common phytobenthos taxa were not restricted to a single substrate but preference was generally high for the artificial substrates, especially brown tiles (mean sp. richness 47). Results of the redundancy analysis (RDA) analysis indicated that ammonium, conductivity, total dissolved solids, salinity, pH, oxygen reduction potential, phosphate and water depth were the major determinants of the phytobenthos composition at the two sites. The spatio–temporal variation of phytoplankton and phytobenthos communities and allochthonous organic matter along the river–estuary continuum was assessed at 8 sites using a combination of community and stable isotope analyses. A total of 178 species belonging to 78 genera were recorded with diatoms being predominant, accounting for 81.9 % of the total abundance. The total chl-a concentration along the river-estuary continuum increased from spring to a high in summer before decreasing to a low in winter. Periphyton communities were observed to be significantly different across sites (p < 0.05) in terms of species richness, abundances and isotopically The high periphytic δ15N values (range 7.9–15.2 ‰) recorded at the downstream sites compared to the pristine upstream sites (δ15N values range 4–7 ‰) suggest nutrient enrichment most likely derived from anthropogenic sources. Overall, our results reveal general patterns of periphyton communities and stable isotopes and provide improved information in the use of periphyton δ15N as an excellent indicator of anthropogenic nitrogen pollution. Ecologists are interested in the factors that control, and the variability in, the contributions of different sources to mixed organic materials traveling through lotic systems. We hypothesized that the source matter fuelling mixed organic pools in a river-estuary continuum varies over space and time, with the upper reaches of a system characterized by allochthonous-dominated material and autochthonous contributions becoming more important in the lower reaches. Samples of the mixed organic pools and allochthonous and autochthonous source materials were collected during the four study periods. The C:N ratios of suspended particulate matter (SPM) collected during summer and winter indicated that the lower reaches of the system had similar organic matter contributions from the freshwater and terrestrial sources. Stable isotope analysis in R revealed that the contributions of autochthonous organic matter were high in SPM along the entire continuum, and aquatic macrophytes were significant contributors to SPM specifically in the upper reaches. The terrestrial leaves made major contributions to the SPM in the middle regions of the system (i.e. downstream sites of the river, particularly in early and late spring). Bulk detritus had large allochthonous matter components in the lower reaches (estuary), and the contributions of aquatic macrophytes and benthic algae to bulk detritus were high (> 50 %) in the upper to middle reaches (river), but low (< 20 %) in the lower reaches (estuary). The current investigation represents the first attempt to assess the validity of the River Continuum Concept (RCC) in a southern African temperate river. The phytoplankton and phytobenthos communities, and chl-a concentration followed a trend similar to that proposed for the river continuum concept (RCC). The middle reaches based on the phytobenthos or phytoplankton communities and chl-a concentrations which were employed as proxies for primary production, were the most productive, while the upper reaches were the least primary productive. The evaluation of organic matter contributions to the SPM and detritus along the river–estuary continuum provided a baseline assessment of the nature and sources of potential food for consumers inhabiting different locations during different times of the year. Incorporating such spatio-temporal variations in SPM and detritus into food web studies will improve our understanding of the flow of carbon through aquatic systems.
- Full Text:
Synthesis and biological evaluation of truncated sarganaphthoquinoic acid derivatives as Hsp90 inhibitors
- Authors: Chiwakata, Maynard T
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64708 , vital:28594
- Description: Hsp90 inhibition has been at the centre of attention in current research due to the possibility of “cracking down” on the entire process leading to the development of malignant cancers. Small underlying principles common in all types of cancers have been determined that govern the transformation of normal human cells into cancerous cells, with all relying on the ATPase activity of Hsp90 protein. Hsp90 protein is therefore an attractive drug target that if successfully inhibited can result in the remission of cancer tumours by one form of treatment. To date, no Hsp90 inhibitor has been sanctioned for cancer treatment as most are still in clinical development. Our research was therefore inspired by reports that indicated the potential of quinones / naphthoquinones to act as Hsp90 inhibitors. Preliminary results of a few selected marine natural product quinone systems i.e. sargaquinoic acid (SQA) (2.47) and lapachol (3.6) showed moderate cytotoxicity and weak interactions with the Hsp90 molecular chaperone, and evidence suggested C-terminal binding of these molecules. No correlation has been determined yet between cytotoxicity and Hsp90 inhibition, hence we aimed to develop natural product inspired molecules that exhibit both cytotoxic and Hsp90 inhibition properties. Due to limited amounts of the natural product that can be acquired from natural sources, synthetic analogues were opted for. Isolation of a few selected quinones was conducted to have material that could be used in biological assays. For structural modifications, a series of truncated naphthoquinone systems were prepared adopting the sarganaphthoquinoic acid (3.5) scaffold. The naphthoquinones were prepared via Diels-Alder reactions of relevant benzoquinones with myrcene, followed by aromatization reactions using MnO2. Various alkyl and aryl amines were then coupled to the C-2/3 position of the naphthoquinone using Michael’s addition reactions. Tricyclic naphthoquinones were also synthesized from reactions with hypotaurine and citral. Design of the analogues incorporated functionalities from known Hsp90 inhibitors e.g. geldanamycin (2.28) and its analogues. Preliminary results obtained showed that coupling of naphthoquinones with aryl-amines resulted in the most cytotoxic compounds (4.14-4.19) with IC50 values as low as 0.3 μM against Hs578T breast cancer carcinoma (triple negative). Most of the alkyl amines (4.20-4.25) had IC50 values greater than 50 μM except for 4.20 and 4.21 that showed IC50 values of 7.6 μM and 2.6 μM respectively. Tricyclic naphthoquinones (4.28-4.29) showed moderate cytotoxic activity of approximately 10 μM. Hsp90 inhibition was assessed by client protein degradation assays, of which SQA (2.47), showed the best Hsp90 inhibition properties, followed by compound 4.20. The most cytotoxic arylamino-naphthoquinone (4.16) and tricyclic naphthoquinones (4.28-4.29) showed only moderate inhibition. None of the compounds led to Hsp70 induction, suggesting possible binding to the C-terminus of Hsp90. Interactions at the binding site were assessed by molecular docking studies and saturation transfer difference (STD) NMR. Docking studies were conducted on the N-terminus of Hsp90 and better binding was observed for arylamino naphthoquinones (4.14-4.19) than for other series of compounds. Unfortunately, the co-crystal structure for the C-terminus of Hsp90 is unavailable, hence docking study comparisons on both domains could not be conducted. However, STD NMR offered a platform to assess binding interactions between the naphthoquinones and the N- or C-terminal domains of Hsp90. However no interactions were observed at both the N- and C- termini of Hsp90 due to either weak binding of ligands to the protein or poor water solubility of the ligands. From these preliminary results, naphthoquinones bind to Hsp90 protein but conclusive remarks to which terminal domain they bind to could not be made. The best candidate from amongst the series of naphthoquinones prepared that showed moderate cytotoxicity and promising Hsp90 inhibition was compound 4.20. We therefore succeeded in developing a new series of naphthoquinones that possess moderate cytotoxicity and show Hsp90 inhibition.
- Full Text:
- Authors: Chiwakata, Maynard T
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64708 , vital:28594
- Description: Hsp90 inhibition has been at the centre of attention in current research due to the possibility of “cracking down” on the entire process leading to the development of malignant cancers. Small underlying principles common in all types of cancers have been determined that govern the transformation of normal human cells into cancerous cells, with all relying on the ATPase activity of Hsp90 protein. Hsp90 protein is therefore an attractive drug target that if successfully inhibited can result in the remission of cancer tumours by one form of treatment. To date, no Hsp90 inhibitor has been sanctioned for cancer treatment as most are still in clinical development. Our research was therefore inspired by reports that indicated the potential of quinones / naphthoquinones to act as Hsp90 inhibitors. Preliminary results of a few selected marine natural product quinone systems i.e. sargaquinoic acid (SQA) (2.47) and lapachol (3.6) showed moderate cytotoxicity and weak interactions with the Hsp90 molecular chaperone, and evidence suggested C-terminal binding of these molecules. No correlation has been determined yet between cytotoxicity and Hsp90 inhibition, hence we aimed to develop natural product inspired molecules that exhibit both cytotoxic and Hsp90 inhibition properties. Due to limited amounts of the natural product that can be acquired from natural sources, synthetic analogues were opted for. Isolation of a few selected quinones was conducted to have material that could be used in biological assays. For structural modifications, a series of truncated naphthoquinone systems were prepared adopting the sarganaphthoquinoic acid (3.5) scaffold. The naphthoquinones were prepared via Diels-Alder reactions of relevant benzoquinones with myrcene, followed by aromatization reactions using MnO2. Various alkyl and aryl amines were then coupled to the C-2/3 position of the naphthoquinone using Michael’s addition reactions. Tricyclic naphthoquinones were also synthesized from reactions with hypotaurine and citral. Design of the analogues incorporated functionalities from known Hsp90 inhibitors e.g. geldanamycin (2.28) and its analogues. Preliminary results obtained showed that coupling of naphthoquinones with aryl-amines resulted in the most cytotoxic compounds (4.14-4.19) with IC50 values as low as 0.3 μM against Hs578T breast cancer carcinoma (triple negative). Most of the alkyl amines (4.20-4.25) had IC50 values greater than 50 μM except for 4.20 and 4.21 that showed IC50 values of 7.6 μM and 2.6 μM respectively. Tricyclic naphthoquinones (4.28-4.29) showed moderate cytotoxic activity of approximately 10 μM. Hsp90 inhibition was assessed by client protein degradation assays, of which SQA (2.47), showed the best Hsp90 inhibition properties, followed by compound 4.20. The most cytotoxic arylamino-naphthoquinone (4.16) and tricyclic naphthoquinones (4.28-4.29) showed only moderate inhibition. None of the compounds led to Hsp70 induction, suggesting possible binding to the C-terminus of Hsp90. Interactions at the binding site were assessed by molecular docking studies and saturation transfer difference (STD) NMR. Docking studies were conducted on the N-terminus of Hsp90 and better binding was observed for arylamino naphthoquinones (4.14-4.19) than for other series of compounds. Unfortunately, the co-crystal structure for the C-terminus of Hsp90 is unavailable, hence docking study comparisons on both domains could not be conducted. However, STD NMR offered a platform to assess binding interactions between the naphthoquinones and the N- or C-terminal domains of Hsp90. However no interactions were observed at both the N- and C- termini of Hsp90 due to either weak binding of ligands to the protein or poor water solubility of the ligands. From these preliminary results, naphthoquinones bind to Hsp90 protein but conclusive remarks to which terminal domain they bind to could not be made. The best candidate from amongst the series of naphthoquinones prepared that showed moderate cytotoxicity and promising Hsp90 inhibition was compound 4.20. We therefore succeeded in developing a new series of naphthoquinones that possess moderate cytotoxicity and show Hsp90 inhibition.
- Full Text:
The associative behaviour of silky sharks, Carcharhinus falciformis, with floating objects in the open ocean
- Authors: Filmalter, John David
- Date: 2015
- Subjects: Silky shark , Silky shark -- Behavior , Silky shark -- Marking , Silky shark -- Food , Tuna fisheries -- Bycatches , Fish aggregation devices , Underwater acoustic telemetry , Fish tagging
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5381 , http://hdl.handle.net/10962/d1018177
- Description: The silky shark Carcharhinus falciformis forms the primary elasmobranch bycatch in tuna purse seine fisheries using fish aggregating devices (FADs) in all of the world’s tropical oceans. Its life-history traits of slow growth, late maturation and low fecundity make it vulnerable to over exploitation, as is apparent from historical bycatch trends. Very little is known about the associative behaviour of this species with floating objects, information which is essential in formulating effective mitigation and management measures. This study aims to address this knowledge gap through the use of various electronic tagging techniques in conjunction with dietary analysis. Dietary data were collected from 323 silky sharks incidentally caught at FADs. Approximately 40 percent of the diet consisted of prey associated with FADs while the remaining 60 percent were non-associated species of crustaceans, cephalopods and fishes. These results suggest that the associative behaviour is not primarily driven by trophic enhancement, but is likely a combination of predator avoidance, social interactions and feeding. Fine-scale behavioural data from silky sharks associated with drifting FADs were collected through the use of acoustic telemetry techniques. Acoustic tags were implanted into 38 silky sharks (69- 116 cm TL) at eight FADs. FADs were equipped with satellite linked acoustic receivers and abandoned to drift freely. Presence/absence and swimming depth data were telemetered via the Iridium satellite system. A total of 300 d of behavioural data were collected from 20 tagged individuals. Individuals remain associated with the same FAD for extended periods (min = 2.84 d, max = 30.60 d, mean = 15.69 d). Strong diel patterns were observed in both association and swimming depth. Typically individuals moved away from FADs after sunset and return later during the night, then remain closely associated until the following evening. Vertical behaviour also changed around sunset with sharks using fairly constant depths, within the upper 25 m, during the day and switching to rapid vertical movements during the night, with dives in excess of 250 m recoded. Broader scale movement behaviour was investigated using pop-up archival satellite tags (PSATs). Tags were deployed on 46 silky sharks (86-224.5 cm TL) for a total of 1495 d. Light data were used to calculate geolocation estimates and reconstruct the sharks’ trajectories. Movement patterns differed between animals and according to deployment duration. Several extensive horizontal movements were observed, with an average track length of 3240 km during an average tag deployment of 44.02 d. Horizontal movement patterns were found to correlate very closely with drift patterns of FADs. Consequently, it appears that the movement behaviour of juvenile silky sharks is strongly influenced by the movement of drifting FADs in this region. Ghost fishing of silky sharks through entanglement in FADs was also investigated using data derived from PSATs as well as underwater visual censuses. Thirteen per cent of the tagged sharks became entangled in FADs and entangled sharks were observed in 35 percent of the 51 FADs surveyed. Using this information in conjunction with estimated time that sharks remained entangled in the FAD (from depth data from PSATs), and scaling up according to estimates of FAD numbers, it was found that between 480 000 and 960 000 silky sharks are killed annually in this manner in the Indian Ocean. Subsequent management measures in this region prevent the deployment of FADs with netting that could lead to entanglement. Overall, floating objects appear to play a significant role in the juvenile life stages of silky sharks in this region. While their association with floating objects is clearly advantageous in an evolutionary sense, under current tuna fishery trends, this behaviour is certainly detrimental for the population.
- Full Text:
- Authors: Filmalter, John David
- Date: 2015
- Subjects: Silky shark , Silky shark -- Behavior , Silky shark -- Marking , Silky shark -- Food , Tuna fisheries -- Bycatches , Fish aggregation devices , Underwater acoustic telemetry , Fish tagging
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5381 , http://hdl.handle.net/10962/d1018177
- Description: The silky shark Carcharhinus falciformis forms the primary elasmobranch bycatch in tuna purse seine fisheries using fish aggregating devices (FADs) in all of the world’s tropical oceans. Its life-history traits of slow growth, late maturation and low fecundity make it vulnerable to over exploitation, as is apparent from historical bycatch trends. Very little is known about the associative behaviour of this species with floating objects, information which is essential in formulating effective mitigation and management measures. This study aims to address this knowledge gap through the use of various electronic tagging techniques in conjunction with dietary analysis. Dietary data were collected from 323 silky sharks incidentally caught at FADs. Approximately 40 percent of the diet consisted of prey associated with FADs while the remaining 60 percent were non-associated species of crustaceans, cephalopods and fishes. These results suggest that the associative behaviour is not primarily driven by trophic enhancement, but is likely a combination of predator avoidance, social interactions and feeding. Fine-scale behavioural data from silky sharks associated with drifting FADs were collected through the use of acoustic telemetry techniques. Acoustic tags were implanted into 38 silky sharks (69- 116 cm TL) at eight FADs. FADs were equipped with satellite linked acoustic receivers and abandoned to drift freely. Presence/absence and swimming depth data were telemetered via the Iridium satellite system. A total of 300 d of behavioural data were collected from 20 tagged individuals. Individuals remain associated with the same FAD for extended periods (min = 2.84 d, max = 30.60 d, mean = 15.69 d). Strong diel patterns were observed in both association and swimming depth. Typically individuals moved away from FADs after sunset and return later during the night, then remain closely associated until the following evening. Vertical behaviour also changed around sunset with sharks using fairly constant depths, within the upper 25 m, during the day and switching to rapid vertical movements during the night, with dives in excess of 250 m recoded. Broader scale movement behaviour was investigated using pop-up archival satellite tags (PSATs). Tags were deployed on 46 silky sharks (86-224.5 cm TL) for a total of 1495 d. Light data were used to calculate geolocation estimates and reconstruct the sharks’ trajectories. Movement patterns differed between animals and according to deployment duration. Several extensive horizontal movements were observed, with an average track length of 3240 km during an average tag deployment of 44.02 d. Horizontal movement patterns were found to correlate very closely with drift patterns of FADs. Consequently, it appears that the movement behaviour of juvenile silky sharks is strongly influenced by the movement of drifting FADs in this region. Ghost fishing of silky sharks through entanglement in FADs was also investigated using data derived from PSATs as well as underwater visual censuses. Thirteen per cent of the tagged sharks became entangled in FADs and entangled sharks were observed in 35 percent of the 51 FADs surveyed. Using this information in conjunction with estimated time that sharks remained entangled in the FAD (from depth data from PSATs), and scaling up according to estimates of FAD numbers, it was found that between 480 000 and 960 000 silky sharks are killed annually in this manner in the Indian Ocean. Subsequent management measures in this region prevent the deployment of FADs with netting that could lead to entanglement. Overall, floating objects appear to play a significant role in the juvenile life stages of silky sharks in this region. While their association with floating objects is clearly advantageous in an evolutionary sense, under current tuna fishery trends, this behaviour is certainly detrimental for the population.
- Full Text: