The call centre labour process : a study of work and workers’ experiences at Joburg Connect, South Africa
- Authors: Magoqwana, Babalwa Mirianda
- Date: 2015
- Subjects: Johannesburg (South Africa) -- Joburg Connect , Call center agents -- South Africa -- Johannesburg -- Case studies , Work environment -- South Africa -- Johannesburg -- Case studies , Customer services -- South Africa -- Johannesburg -- Case studies , Personnel management -- South Africa -- Johannesburg -- Case studies , Employee morale -- South Africa -- Johannesburg -- Case studies , Employees -- Attitudes -- Case studies , Municipal services -- South Africa -- Johannesburg -- Case studies , South Africa -- Economic conditions -- 1991- , South Africa -- Politics and government -- 1994- , South Africa -- Social conditions -- 1994-
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3400 , http://hdl.handle.net/10962/d1018569 , Johannesburg (South Africa) -- Joburg Connect , Call center agents -- South Africa -- Johannesburg -- Case studies , Work environment -- South Africa -- Johannesburg -- Case studies , Customer services -- South Africa -- Johannesburg -- Case studies , Personnel management -- South Africa -- Johannesburg -- Case studies , Employee morale -- South Africa -- Johannesburg -- Case studies , Employees -- Attitudes -- Case studies , Municipal services -- South Africa -- Johannesburg -- Case studies , South Africa -- Economic conditions -- 1991- , South Africa -- Politics and government -- 1994-
- Description: This thesis is an investigation of the call centre labour process focusing on both the way work is organised and experienced by the operators and the management of employee relations inside a local government workplace – The City of Johannesburg (COJ) Call Centre. The City of Johannesburg is used to understand labour process dynamics in local government. Johannesburg was recently named the ‘World’s Second Most Inspiring City’ (by Global City Index, 2014) possessing economic and political influence within and around South Africa. Johannesburg is the economic hub of the country, contributing nine per cent to the South African GDP. But it needs to be emphasised that the focus of this thesis is on the labour process rather than the City of Johannesburg. The nature of work in the COJ local government call centre involves answering calls about water, electricity, refuse removal, accounts and other general municipal services. The government’s objective was to have a more customer centred service delivery plan in compliance with the Batho Pele (People first) framework (RSA, 1997, White Paper on Local Government). This resulted in a shift in the government ‘mantra’ and philosophy from viewing Johannesburg residents as customers rather than citizens. This shift from public administration to New Public Management (NPM) has meant the introduction of private sector business principles into public services. This seeks to improve organisational and individual performance to enhance service delivery within South African municipalities. This is also meant to promote responsive and performance management culture. The study uses Labour Process Theory set out in Labour and Monopoly Capital (LMC-1974) by Harry Braverman to argue for the need to contextualise the labour process debate within the socio-political understanding of work in South Africa. In developing the argument about the narrow analysis of public sector call centres based on economic lines I have reviewed literature on call centres which is influenced by Michel Foucault and Harry Braverman to understand the public sector call centre labour process. Inspired by Adesina (2002) and Mafeje (1981) this study uses a ‘complementarity’ approach to knowledge. This means the local context tends to complement the global trends though not without contradictions within the local context. The case study approach was used for in-depth analysis of the local context through different methods (semi-structured interviews, focus group discussions, observations and survey questionnaires) to collect data from different stakeholders within the City of Johannesburg between 2010 and 2013. The concept of a ‘(dis)connecting city’ is fitting for the COJ as this city connects the rest of Sub-Saharan Africa and South Africa to the world through its commerce and political influence. Based on the challenges experienced in this local government call centre I attempt to show the the politicised nature of the public sector call centre labour process. I use the differential power relations in the frontline between customer and Joburg connect workers to illustrate this point. The lack of emphasis on the politicised nature of public sector call centres within literature is informed by the de-contextualised nature of these call centres. In the end, I argue that public sector call centre labour process is multilayered, contradictory and complicated because of these workers’ role as citizens, customers, community members and service providers from a politicised community environment.
- Full Text:
- Authors: Magoqwana, Babalwa Mirianda
- Date: 2015
- Subjects: Johannesburg (South Africa) -- Joburg Connect , Call center agents -- South Africa -- Johannesburg -- Case studies , Work environment -- South Africa -- Johannesburg -- Case studies , Customer services -- South Africa -- Johannesburg -- Case studies , Personnel management -- South Africa -- Johannesburg -- Case studies , Employee morale -- South Africa -- Johannesburg -- Case studies , Employees -- Attitudes -- Case studies , Municipal services -- South Africa -- Johannesburg -- Case studies , South Africa -- Economic conditions -- 1991- , South Africa -- Politics and government -- 1994- , South Africa -- Social conditions -- 1994-
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3400 , http://hdl.handle.net/10962/d1018569 , Johannesburg (South Africa) -- Joburg Connect , Call center agents -- South Africa -- Johannesburg -- Case studies , Work environment -- South Africa -- Johannesburg -- Case studies , Customer services -- South Africa -- Johannesburg -- Case studies , Personnel management -- South Africa -- Johannesburg -- Case studies , Employee morale -- South Africa -- Johannesburg -- Case studies , Employees -- Attitudes -- Case studies , Municipal services -- South Africa -- Johannesburg -- Case studies , South Africa -- Economic conditions -- 1991- , South Africa -- Politics and government -- 1994-
- Description: This thesis is an investigation of the call centre labour process focusing on both the way work is organised and experienced by the operators and the management of employee relations inside a local government workplace – The City of Johannesburg (COJ) Call Centre. The City of Johannesburg is used to understand labour process dynamics in local government. Johannesburg was recently named the ‘World’s Second Most Inspiring City’ (by Global City Index, 2014) possessing economic and political influence within and around South Africa. Johannesburg is the economic hub of the country, contributing nine per cent to the South African GDP. But it needs to be emphasised that the focus of this thesis is on the labour process rather than the City of Johannesburg. The nature of work in the COJ local government call centre involves answering calls about water, electricity, refuse removal, accounts and other general municipal services. The government’s objective was to have a more customer centred service delivery plan in compliance with the Batho Pele (People first) framework (RSA, 1997, White Paper on Local Government). This resulted in a shift in the government ‘mantra’ and philosophy from viewing Johannesburg residents as customers rather than citizens. This shift from public administration to New Public Management (NPM) has meant the introduction of private sector business principles into public services. This seeks to improve organisational and individual performance to enhance service delivery within South African municipalities. This is also meant to promote responsive and performance management culture. The study uses Labour Process Theory set out in Labour and Monopoly Capital (LMC-1974) by Harry Braverman to argue for the need to contextualise the labour process debate within the socio-political understanding of work in South Africa. In developing the argument about the narrow analysis of public sector call centres based on economic lines I have reviewed literature on call centres which is influenced by Michel Foucault and Harry Braverman to understand the public sector call centre labour process. Inspired by Adesina (2002) and Mafeje (1981) this study uses a ‘complementarity’ approach to knowledge. This means the local context tends to complement the global trends though not without contradictions within the local context. The case study approach was used for in-depth analysis of the local context through different methods (semi-structured interviews, focus group discussions, observations and survey questionnaires) to collect data from different stakeholders within the City of Johannesburg between 2010 and 2013. The concept of a ‘(dis)connecting city’ is fitting for the COJ as this city connects the rest of Sub-Saharan Africa and South Africa to the world through its commerce and political influence. Based on the challenges experienced in this local government call centre I attempt to show the the politicised nature of the public sector call centre labour process. I use the differential power relations in the frontline between customer and Joburg connect workers to illustrate this point. The lack of emphasis on the politicised nature of public sector call centres within literature is informed by the de-contextualised nature of these call centres. In the end, I argue that public sector call centre labour process is multilayered, contradictory and complicated because of these workers’ role as citizens, customers, community members and service providers from a politicised community environment.
- Full Text:
The detection of glyphosate and glyphosate-based herbicides in water, using nanotechnology
- De Almeida, Louise Kashiyavala Sophia
- Authors: De Almeida, Louise Kashiyavala Sophia
- Date: 2015
- Subjects: Water -- Glyphosate content , Aquatic herbicides -- South Africa , Aquatic herbicides -- Physiological effect , Nanotechnology , Invasive plants -- South Africa , Genetic toxicology , Thiazoles , Tetrazolium , Immunotoxicology , Colorimetry , Nanofibers
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4163 , http://hdl.handle.net/10962/d1019755
- Description: Glyphosate (N-phosphonomethylglycine) is an organophosphate compound which was developed by the Monsanto Company in 1971 and is the active ingredient found in several herbicide formulations. The use of glyphosate-based herbicides in South Africa for the control of alien invasive plants and weeds is well established, extensive and currently unregulated, which vastly increases the likelihood of glyphosate contamination in environmental water systems. Although the use of glyphosate-based herbicides is required for economic enhancement in industries such as agriculture, the presence of this compound in natural water systems presents a potential risk to human health. Glyphosate and glyphosate formulations were previously considered safe, however their toxicity has become a major focal point of research over recent years. The lack of monitoring protocols for pesticides in South Africa is primarily due to limited financial capacity and the lack of analytical techniques.
- Full Text:
- Authors: De Almeida, Louise Kashiyavala Sophia
- Date: 2015
- Subjects: Water -- Glyphosate content , Aquatic herbicides -- South Africa , Aquatic herbicides -- Physiological effect , Nanotechnology , Invasive plants -- South Africa , Genetic toxicology , Thiazoles , Tetrazolium , Immunotoxicology , Colorimetry , Nanofibers
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4163 , http://hdl.handle.net/10962/d1019755
- Description: Glyphosate (N-phosphonomethylglycine) is an organophosphate compound which was developed by the Monsanto Company in 1971 and is the active ingredient found in several herbicide formulations. The use of glyphosate-based herbicides in South Africa for the control of alien invasive plants and weeds is well established, extensive and currently unregulated, which vastly increases the likelihood of glyphosate contamination in environmental water systems. Although the use of glyphosate-based herbicides is required for economic enhancement in industries such as agriculture, the presence of this compound in natural water systems presents a potential risk to human health. Glyphosate and glyphosate formulations were previously considered safe, however their toxicity has become a major focal point of research over recent years. The lack of monitoring protocols for pesticides in South Africa is primarily due to limited financial capacity and the lack of analytical techniques.
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The development of amine-based extractants for separation of base metals in a sulfate medium
- Authors: Magwa, Nomampondo Penelope
- Date: 2015
- Subjects: Extraction (Chemistry) , Sulfates , Ligands , Benzimidazoles , Infrared spectroscopy , Nuclear magnetic resonance spectroscopy , Metal ions , Metals
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4559 , http://hdl.handle.net/10962/d1020010
- Description: Tridentate benzimidazole-based ligands, bis((1H-benzimidazol-2-yl)methyl)sulfide (BNSN) and bis((1H-benzimidazol-2-yl)methyl)amine (BNNN), along with dinonylnaphthalene sulfonic acid (DNNSA) as a synergist, were investigated as potential selective extractants for Ni2+ from base metals in a solvent extraction system using 2-octanol/Shellsol 2325 (8:2) as diluent and modifier. However, extraction studies show a lack of pH-metric separation of the later 3d metal ions with bis((1-octylbenzimidazol-2-yl)methyl)sulfide (BONSN) and bis((1- decylbenzimidazol-2-yl)methyl)amine (BDNNN) as extractants, but extractions occurred in the low pH range with an opportunity for back extraction. This investigation suggested that tridentate ligands (at least those of the nature investigated here) are not feasible extractants for separation of base metal ions due to their lack of stereochemical “tailor-making.”
- Full Text:
- Authors: Magwa, Nomampondo Penelope
- Date: 2015
- Subjects: Extraction (Chemistry) , Sulfates , Ligands , Benzimidazoles , Infrared spectroscopy , Nuclear magnetic resonance spectroscopy , Metal ions , Metals
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4559 , http://hdl.handle.net/10962/d1020010
- Description: Tridentate benzimidazole-based ligands, bis((1H-benzimidazol-2-yl)methyl)sulfide (BNSN) and bis((1H-benzimidazol-2-yl)methyl)amine (BNNN), along with dinonylnaphthalene sulfonic acid (DNNSA) as a synergist, were investigated as potential selective extractants for Ni2+ from base metals in a solvent extraction system using 2-octanol/Shellsol 2325 (8:2) as diluent and modifier. However, extraction studies show a lack of pH-metric separation of the later 3d metal ions with bis((1-octylbenzimidazol-2-yl)methyl)sulfide (BONSN) and bis((1- decylbenzimidazol-2-yl)methyl)amine (BDNNN) as extractants, but extractions occurred in the low pH range with an opportunity for back extraction. This investigation suggested that tridentate ligands (at least those of the nature investigated here) are not feasible extractants for separation of base metal ions due to their lack of stereochemical “tailor-making.”
- Full Text:
The diversity of root fungi associated with Erica species occurring in the Albany Centre of Endemism
- Authors: Bizabani, Christine
- Date: 2015
- Subjects: Ericaceae , Ericas , Roots (Botany) -- Diseases and pests , Mycorrhizal fungi , Polymerase chain reaction , Fungi -- Classification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4160 , http://hdl.handle.net/10962/d1018575
- Description: South Africa has the highest species diversity of ericaceous plants belonging to the Erica genus. There are over 850 identified species in the Cape Floral Region. The Albany Centre of Endemism (ACOE) is located within this region and is a hotspot of diversity consisting of various plant genera. The success of Erica plants is ubiquitously attributed to mycorrhizal relationships they engage in with a diverse group of fungi. This symbiosis is known as the ericoid mycorrhizal (ERM) association. The overall aim of this study was to establish the diversity of root fungi associated with Erica plants using morphological, molecular and 454 pyrosequencing techniques. Six Erica species were identified using leaf and flower morphology according to taxonomic keys. The identified plants were Erica cerinthoides, Erica demissa, Erica chamissonis, Erica glumiflora, Erica caffra and Erica nemorosa. Roots from sampled plants were stained and examined microscopically to determine their mycorrhizal status. Ericoid mycorrhizal associations together with dark septate endophyte (DSE) structures and hyphae that did not form any specific structure were observed in all the roots. In addition arbuscular mycorrhizal (AM) structures in the form of vesicles were detected in E. glumiflora and E. cerinthoides. In order to identify the culturable fungi associated with the respective hosts, sterilised roots were placed on various culture media for cultivation. Thereafter isolated fungi were morphologically classified into 67 morphotypes. These were mostly sterile and darkly pigmented. Non-sporulating mycelia of variable colouration such as white, cream-yellowish, beige, green and brown were also observed. Further identification was carried out using molecular techniques. DNA was extracted separately from pure cultures and amplified using ITS1 and ITS4 primers in a polymerase chain reaction (PCR). Thereafter sequencing and Basic Local Alignment Search Tool (BLAST) were used to identify the isolates to generic level. The fungi were taxonomically classified into 54 operational taxonomic units and 94 percent were Ascomycetes and Helotiales was the dominant order. Unclassified Helotiales with affinities to fungi currently identified as Epacrid root fungus was common in all hosts. Other isolates that were identified included Oidiodendron, Meliniomyces, Phialocephala, Cadophora, Lachnum, Leohumicola Cryptosporiopsis, Chaetomium, Acremonium and Epicoccum species. Basidiomycetes were represented by two OTUs belonging to the genus Mycena. Four OTUs comprised fungi that had no significant alignments in the reference databases. Direct root DNA extraction together with 454 pyrosequencing was used to detect the diversity of culturable and unculturable fungi associated with the identified hosts. The ITS2 region was targeted for sequencing. Although Ascomycetes remained the dominant phyla, Basidiomycetes were also detected in all host plants. Glomeromycota was present in E. caffra and E. cerinthoides. Helotiales was dominant in all Erica plants with the exception of E. cerinthoides and E. chamissonis which were dominated by the order Chaetothyriales. The OTUs identified to genus level included Epacris pulchella root fungus, Oidiodendron cf. maius, Acremonium implicatum, Leohumicola, Lachnum, Capronia and Mycena species. Culture-based techniques and pyrosequencing detected similar fungal composition comprising Ascomycetes, while, pyrosequencing was able to detect Glomeromycetes and Basidiomycetes.
- Full Text:
The diversity of root fungi associated with Erica species occurring in the Albany Centre of Endemism
- Authors: Bizabani, Christine
- Date: 2015
- Subjects: Ericaceae , Ericas , Roots (Botany) -- Diseases and pests , Mycorrhizal fungi , Polymerase chain reaction , Fungi -- Classification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4160 , http://hdl.handle.net/10962/d1018575
- Description: South Africa has the highest species diversity of ericaceous plants belonging to the Erica genus. There are over 850 identified species in the Cape Floral Region. The Albany Centre of Endemism (ACOE) is located within this region and is a hotspot of diversity consisting of various plant genera. The success of Erica plants is ubiquitously attributed to mycorrhizal relationships they engage in with a diverse group of fungi. This symbiosis is known as the ericoid mycorrhizal (ERM) association. The overall aim of this study was to establish the diversity of root fungi associated with Erica plants using morphological, molecular and 454 pyrosequencing techniques. Six Erica species were identified using leaf and flower morphology according to taxonomic keys. The identified plants were Erica cerinthoides, Erica demissa, Erica chamissonis, Erica glumiflora, Erica caffra and Erica nemorosa. Roots from sampled plants were stained and examined microscopically to determine their mycorrhizal status. Ericoid mycorrhizal associations together with dark septate endophyte (DSE) structures and hyphae that did not form any specific structure were observed in all the roots. In addition arbuscular mycorrhizal (AM) structures in the form of vesicles were detected in E. glumiflora and E. cerinthoides. In order to identify the culturable fungi associated with the respective hosts, sterilised roots were placed on various culture media for cultivation. Thereafter isolated fungi were morphologically classified into 67 morphotypes. These were mostly sterile and darkly pigmented. Non-sporulating mycelia of variable colouration such as white, cream-yellowish, beige, green and brown were also observed. Further identification was carried out using molecular techniques. DNA was extracted separately from pure cultures and amplified using ITS1 and ITS4 primers in a polymerase chain reaction (PCR). Thereafter sequencing and Basic Local Alignment Search Tool (BLAST) were used to identify the isolates to generic level. The fungi were taxonomically classified into 54 operational taxonomic units and 94 percent were Ascomycetes and Helotiales was the dominant order. Unclassified Helotiales with affinities to fungi currently identified as Epacrid root fungus was common in all hosts. Other isolates that were identified included Oidiodendron, Meliniomyces, Phialocephala, Cadophora, Lachnum, Leohumicola Cryptosporiopsis, Chaetomium, Acremonium and Epicoccum species. Basidiomycetes were represented by two OTUs belonging to the genus Mycena. Four OTUs comprised fungi that had no significant alignments in the reference databases. Direct root DNA extraction together with 454 pyrosequencing was used to detect the diversity of culturable and unculturable fungi associated with the identified hosts. The ITS2 region was targeted for sequencing. Although Ascomycetes remained the dominant phyla, Basidiomycetes were also detected in all host plants. Glomeromycota was present in E. caffra and E. cerinthoides. Helotiales was dominant in all Erica plants with the exception of E. cerinthoides and E. chamissonis which were dominated by the order Chaetothyriales. The OTUs identified to genus level included Epacris pulchella root fungus, Oidiodendron cf. maius, Acremonium implicatum, Leohumicola, Lachnum, Capronia and Mycena species. Culture-based techniques and pyrosequencing detected similar fungal composition comprising Ascomycetes, while, pyrosequencing was able to detect Glomeromycetes and Basidiomycetes.
- Full Text:
The isolation, characterisation and chemotaxonomic significance of secondary metabolites from selected South African Laurencia spp. Rhodophyta
- Authors: Fakee, Jameel
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64696 , vital:28593
- Description: Bioprospection of marine organisms as a potential source for lead drugs is becoming increasingly popular. The secondary metabolome of these organisms consists of structurally diverse molecules possessing unprecedented carbon skeletons, the biosynthesis of which occurs via complex metabolomic pathways driven by specialist enzymes. This structural novelty is highly influential on the favourable bioactivity these compounds display. A prominent example of such a compound is trabectedin marketed as Yondelis®. Registered for the treatment of soft tissue sarcomas, this marine drug was developed from extracts of the tunicate Ecteinascidia turbinata. South Africa is renowned for possessing a highly diverse marine biota including several endemic species of marine red algae belonging to the Laurencia sensu stricto genus, which falls within the Laurencia complex. Despite having a good reputation for fascinating secondary metabolites, the taxonomy of Laurencia natural products is proving challenging for reasons including the presence of cryptic species, as well as individual species displaying morphological variability. The aim of this study was thus to isolate secondary metabolites from various South African Laurencia spp. and subsequently assess their chemotaxonomic significance by analysis of a parallel plastid rbcL phylogeny study of Laurencia spp. This study reports the first phycochemical investigation into Laurencia natalensis Kylin, Laurencia cf. corymbosa J.Agardh, Laurencia complanata (Suhr) Kützing, Laurencia sodwaniensis Francis, Bolton, Mattio and Anderson submitted, Laurencia multiclavata Francis, Bolton, Mattio and Anderson submitted, and a South African specimen of Laurenciella marilzae Gil-Rodríguez, Sentíes, Díaz-Larrea, Cassano and M.T. Fujii (basionym: Laurencia marilzae) originally described from Spain. Additionally, the chemical profiles of previously explored species Laurencia flexuosa Kützing and Laurencia glomerata Kützing were re-investigated. The organic extracts of the above species afforded 31 compounds belonging to a wide array of structural classes including halo-chamigranes, linear C15 acetogenins, indole alkaloids, cuparanes and cyclic bromo-ethers. A new tri-cyclic keto-cuparane (4.4) was isolated from L.cf. corymbosa alongside the new cuparanes 4.1 and 4.7. Algoane (5.9), a unique marker compound isolated from L. natalensis, was previously only reported from a sea-hare. Such marker compounds which are exclusive to an individual algal species increase the ease of their subsequent identification. The feasibility of chemotaxonomy as an additional tool to classify Laurencia spp. Was established as broad predictions of a specimen’s phylogeny, based on representatives of its secondary metabolome, proved viable. The study specimens were shown to possess similar chemical profiles to their sister species e.g. L. complanata, L. sodwaniensis and L. multiclavata produced similar metabolites to their sister species as inferred by an rbcL phylogeny tree. Finally, a 1H NMR profiling study on the crude organic extracts of various Laurencia spp. generated distinctive, reproducible spectra, exposing the value of NMR spectroscopy as a rudimentary species discernment tool.
- Full Text:
- Authors: Fakee, Jameel
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64696 , vital:28593
- Description: Bioprospection of marine organisms as a potential source for lead drugs is becoming increasingly popular. The secondary metabolome of these organisms consists of structurally diverse molecules possessing unprecedented carbon skeletons, the biosynthesis of which occurs via complex metabolomic pathways driven by specialist enzymes. This structural novelty is highly influential on the favourable bioactivity these compounds display. A prominent example of such a compound is trabectedin marketed as Yondelis®. Registered for the treatment of soft tissue sarcomas, this marine drug was developed from extracts of the tunicate Ecteinascidia turbinata. South Africa is renowned for possessing a highly diverse marine biota including several endemic species of marine red algae belonging to the Laurencia sensu stricto genus, which falls within the Laurencia complex. Despite having a good reputation for fascinating secondary metabolites, the taxonomy of Laurencia natural products is proving challenging for reasons including the presence of cryptic species, as well as individual species displaying morphological variability. The aim of this study was thus to isolate secondary metabolites from various South African Laurencia spp. and subsequently assess their chemotaxonomic significance by analysis of a parallel plastid rbcL phylogeny study of Laurencia spp. This study reports the first phycochemical investigation into Laurencia natalensis Kylin, Laurencia cf. corymbosa J.Agardh, Laurencia complanata (Suhr) Kützing, Laurencia sodwaniensis Francis, Bolton, Mattio and Anderson submitted, Laurencia multiclavata Francis, Bolton, Mattio and Anderson submitted, and a South African specimen of Laurenciella marilzae Gil-Rodríguez, Sentíes, Díaz-Larrea, Cassano and M.T. Fujii (basionym: Laurencia marilzae) originally described from Spain. Additionally, the chemical profiles of previously explored species Laurencia flexuosa Kützing and Laurencia glomerata Kützing were re-investigated. The organic extracts of the above species afforded 31 compounds belonging to a wide array of structural classes including halo-chamigranes, linear C15 acetogenins, indole alkaloids, cuparanes and cyclic bromo-ethers. A new tri-cyclic keto-cuparane (4.4) was isolated from L.cf. corymbosa alongside the new cuparanes 4.1 and 4.7. Algoane (5.9), a unique marker compound isolated from L. natalensis, was previously only reported from a sea-hare. Such marker compounds which are exclusive to an individual algal species increase the ease of their subsequent identification. The feasibility of chemotaxonomy as an additional tool to classify Laurencia spp. Was established as broad predictions of a specimen’s phylogeny, based on representatives of its secondary metabolome, proved viable. The study specimens were shown to possess similar chemical profiles to their sister species e.g. L. complanata, L. sodwaniensis and L. multiclavata produced similar metabolites to their sister species as inferred by an rbcL phylogeny tree. Finally, a 1H NMR profiling study on the crude organic extracts of various Laurencia spp. generated distinctive, reproducible spectra, exposing the value of NMR spectroscopy as a rudimentary species discernment tool.
- Full Text:
The relationship between leadership and organisational effectiveness among indigenous banks in Kenya
- Authors: Muriithi, Samuel Muiruri
- Date: 2015
- Subjects: Leadership -- Kenya , Organizational effectiveness -- Kenya , Transformational leadership -- Kenya , Strategic planning -- Kenya , Community banks -- Kenya , Structural equation modeling , Emotional intelligence
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1208 , http://hdl.handle.net/10962/d1020015
- Description: Defining leadership effectiveness (LE) remains a controversial subject with scholars and researchers disagreeing on a common definition. Most studies term leadership effectiveness as the leaders’ ability to successfully exercise personal influence and abilities in order to accomplish set standards. Such leadership effectiveness was measured using three elements, namely influence, follow commitment and versatility. To be effective, leaders must possess certain competencies and perform specific tasks (independent variables). In this study, three leadership competencies, namely strategic thinking, emotional effectiveness and transformational leadership were examined. Similarly, seven leadership tasks (set organisational direction, develop human capital, build core competencies, create organisational alignment, sustain appropriate organisational culture, manage change and establish balanced organisational control) were identified as essential for the attainment of leadership effectiveness (intervening variable) which, in turn, leads to organisational effectiveness (dependent variable). Given the importance of leadership effectiveness to organisational effectiveness, the purpose of this study was to identify, investigate and empirically test the possible relationship between leadership effectiveness and organisational effectiveness within the Kenyan indigenous banks. To achieve the aim of the study, a survey was undertaken using a structured self-administered questionnaire. The respondents were identified using both probability and non-probability techniques with the survey yielding 257 usable questionnaires that were statistically analysed. The proposed hypothetical relationship between leadership effectiveness and organisational effectiveness was assessed using Structural Equation Modelling (SEM), a multivariate statistical technique. The SEM included Cronbach’s alpha and confirmatory factor analyses to assess the dicriminant reliability and validity of the measuring instrument, and Goodness-of-fit indices. The study findings demonstrated the existence of a strong relationship between leadership competencies, tasks performed and leadership effectiveness which in turn impacts on organisational effectiveness, in this case the overall effectiveness of the Kenyan indigenous banks.
- Full Text:
The relationship between leadership and organisational effectiveness among indigenous banks in Kenya
- Authors: Muriithi, Samuel Muiruri
- Date: 2015
- Subjects: Leadership -- Kenya , Organizational effectiveness -- Kenya , Transformational leadership -- Kenya , Strategic planning -- Kenya , Community banks -- Kenya , Structural equation modeling , Emotional intelligence
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1208 , http://hdl.handle.net/10962/d1020015
- Description: Defining leadership effectiveness (LE) remains a controversial subject with scholars and researchers disagreeing on a common definition. Most studies term leadership effectiveness as the leaders’ ability to successfully exercise personal influence and abilities in order to accomplish set standards. Such leadership effectiveness was measured using three elements, namely influence, follow commitment and versatility. To be effective, leaders must possess certain competencies and perform specific tasks (independent variables). In this study, three leadership competencies, namely strategic thinking, emotional effectiveness and transformational leadership were examined. Similarly, seven leadership tasks (set organisational direction, develop human capital, build core competencies, create organisational alignment, sustain appropriate organisational culture, manage change and establish balanced organisational control) were identified as essential for the attainment of leadership effectiveness (intervening variable) which, in turn, leads to organisational effectiveness (dependent variable). Given the importance of leadership effectiveness to organisational effectiveness, the purpose of this study was to identify, investigate and empirically test the possible relationship between leadership effectiveness and organisational effectiveness within the Kenyan indigenous banks. To achieve the aim of the study, a survey was undertaken using a structured self-administered questionnaire. The respondents were identified using both probability and non-probability techniques with the survey yielding 257 usable questionnaires that were statistically analysed. The proposed hypothetical relationship between leadership effectiveness and organisational effectiveness was assessed using Structural Equation Modelling (SEM), a multivariate statistical technique. The SEM included Cronbach’s alpha and confirmatory factor analyses to assess the dicriminant reliability and validity of the measuring instrument, and Goodness-of-fit indices. The study findings demonstrated the existence of a strong relationship between leadership competencies, tasks performed and leadership effectiveness which in turn impacts on organisational effectiveness, in this case the overall effectiveness of the Kenyan indigenous banks.
- Full Text:
The supervisor’s tale: postgraduate supervisors’ experiences in a changing Higher Education environment
- Authors: Searle, Ruth Lesley
- Date: 2015
- Subjects: Graduate students -- Supervision of -- South Africa , Education, Higher -- South Africa , Educational change -- South Africa , Archer, Margaret Scotford -- Political and social views , Critical realism , Knowledge, Sociology of , Dissertations, Academic , Faculty advisors -- South Africa , Education -- Study and teaching (Graduate) -- South Africa , Universities and colleges -- Graduate work
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1331 , http://hdl.handle.net/10962/d1019952
- Description: The environment in which higher education institutions operate is changing, and these changes are impacting on all aspects of higher education, including postgraduate levels. Changes wrought by globalisation, heralded by rapid advances in technology have inaugurated a new era in which there are long term consequences for higher education. The shift towards more quantitative and measurable "outputs" signifies a fundamental change in the educational ethos in institutions. Effectiveness is now judged primarily on numbers of graduates and publications rather than on other aspects. The drive is to produce a highly educated population, especially through increasing postgraduates who can drive national innovation and improve national economies. This affects academics in a range of ways, not least in the ways in which they engage in teaching, what they are willing to do and how they do it. Such changes influence the kinds of research done, the structures and funding which support research, and thus naturally shapes the kinds of postgraduate programmes and teaching that occurs. This study, situated in the field of Higher Education Studies, adopting a critical realist stance and drawing on the social theory of Margaret Archer and the concepts of expert and novice, explores the experiences of postgraduate supervisors from one South African institution across a range of disciplines. Individual experiences at the level of the Empirical and embodied in practice at the level of the Actual allow for the identification of possible mechanisms at the level of the Real which structure the sector. The research design then allows for an exploration across mezzo, macro and micro levels. Individuals outline their own particular situations, identifying a number of elements which enabled or constrained them and how, in exercising their agency, they develop their strategies for supervision drawing on a range of different resources that they identify and that may be available to them. Student characteristics, discipline status and placement, funding, and the emergent policy environment are all identified as influencing their practice. In some instances supervisors recognise the broader influences on the system that involve them in their undertaking, noting the international trends. Through their narratives and the discourses they engage a number of contradictions that have developed in the system with growing neo-liberal trends and vocationalism highlighting tensions between academic freedom and autonomy, and demands for productivity, efficiency and compliance, and between an educational focus and a training bias in particular along with others. Especially notable is how this contributes to the current ideologies surrounding knowledge and knowledge production. Their individual interests and concerns, and emergent academic identities as they take shape over time, also modifies the process and how individual supervisors influence their own environments in agentic moves becomes apparent. Whilst often individuals highlight the lack of support especially in the early phases of supervision, the emergent policy-constrained environment is also seen as curtailing possibilities and especially in limiting the possibilities for the exercise of agency. Whilst the study has some limitations in the range and number of respondents nevertheless the data provided rich evidence of how individual supervisors are affected, and how they respond in varied conditions. What is highlighted through these experiences are ways pressures are increasing for both supervisors and students and changing how they engage. Concerns in particular are raised about the growing functional and instrumental nature of the process with an emphasis on the effects on the kinds of researchers being developed and the knowledge that is therefore being produced. As costs increase for academics through the environments developed and with the varied roles they take on so they become more selective and reluctant to expand the role. This research has provided insights into ideas, beliefs and values relating to the postgraduate sector and to the process of postgraduate supervision and how it occurs. This includes the structures and cultural conditions that enable or constrain practitioners as they develop in the role in this particular institution. It has explored some of the ways that mechanisms at international, national and institutional levels shape the role and practices of supervisors. The effects of mechanisms are in no way a given or simply understood. In this way the research may contribute to more emancipatory knowledge which could be used in planning and deciding on emergent policies and practices which might create a more supportive and creative postgraduate environment.
- Full Text:
- Authors: Searle, Ruth Lesley
- Date: 2015
- Subjects: Graduate students -- Supervision of -- South Africa , Education, Higher -- South Africa , Educational change -- South Africa , Archer, Margaret Scotford -- Political and social views , Critical realism , Knowledge, Sociology of , Dissertations, Academic , Faculty advisors -- South Africa , Education -- Study and teaching (Graduate) -- South Africa , Universities and colleges -- Graduate work
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1331 , http://hdl.handle.net/10962/d1019952
- Description: The environment in which higher education institutions operate is changing, and these changes are impacting on all aspects of higher education, including postgraduate levels. Changes wrought by globalisation, heralded by rapid advances in technology have inaugurated a new era in which there are long term consequences for higher education. The shift towards more quantitative and measurable "outputs" signifies a fundamental change in the educational ethos in institutions. Effectiveness is now judged primarily on numbers of graduates and publications rather than on other aspects. The drive is to produce a highly educated population, especially through increasing postgraduates who can drive national innovation and improve national economies. This affects academics in a range of ways, not least in the ways in which they engage in teaching, what they are willing to do and how they do it. Such changes influence the kinds of research done, the structures and funding which support research, and thus naturally shapes the kinds of postgraduate programmes and teaching that occurs. This study, situated in the field of Higher Education Studies, adopting a critical realist stance and drawing on the social theory of Margaret Archer and the concepts of expert and novice, explores the experiences of postgraduate supervisors from one South African institution across a range of disciplines. Individual experiences at the level of the Empirical and embodied in practice at the level of the Actual allow for the identification of possible mechanisms at the level of the Real which structure the sector. The research design then allows for an exploration across mezzo, macro and micro levels. Individuals outline their own particular situations, identifying a number of elements which enabled or constrained them and how, in exercising their agency, they develop their strategies for supervision drawing on a range of different resources that they identify and that may be available to them. Student characteristics, discipline status and placement, funding, and the emergent policy environment are all identified as influencing their practice. In some instances supervisors recognise the broader influences on the system that involve them in their undertaking, noting the international trends. Through their narratives and the discourses they engage a number of contradictions that have developed in the system with growing neo-liberal trends and vocationalism highlighting tensions between academic freedom and autonomy, and demands for productivity, efficiency and compliance, and between an educational focus and a training bias in particular along with others. Especially notable is how this contributes to the current ideologies surrounding knowledge and knowledge production. Their individual interests and concerns, and emergent academic identities as they take shape over time, also modifies the process and how individual supervisors influence their own environments in agentic moves becomes apparent. Whilst often individuals highlight the lack of support especially in the early phases of supervision, the emergent policy-constrained environment is also seen as curtailing possibilities and especially in limiting the possibilities for the exercise of agency. Whilst the study has some limitations in the range and number of respondents nevertheless the data provided rich evidence of how individual supervisors are affected, and how they respond in varied conditions. What is highlighted through these experiences are ways pressures are increasing for both supervisors and students and changing how they engage. Concerns in particular are raised about the growing functional and instrumental nature of the process with an emphasis on the effects on the kinds of researchers being developed and the knowledge that is therefore being produced. As costs increase for academics through the environments developed and with the varied roles they take on so they become more selective and reluctant to expand the role. This research has provided insights into ideas, beliefs and values relating to the postgraduate sector and to the process of postgraduate supervision and how it occurs. This includes the structures and cultural conditions that enable or constrain practitioners as they develop in the role in this particular institution. It has explored some of the ways that mechanisms at international, national and institutional levels shape the role and practices of supervisors. The effects of mechanisms are in no way a given or simply understood. In this way the research may contribute to more emancipatory knowledge which could be used in planning and deciding on emergent policies and practices which might create a more supportive and creative postgraduate environment.
- Full Text:
The use of hydrological information to improve flood management-integrated hydrological modelling of the Zambezi River basin
- Vilanculos, Agostinho Chuquelane Fadulo
- Authors: Vilanculos, Agostinho Chuquelane Fadulo
- Date: 2015
- Subjects: Hydrologic models -- Zambezi River Watershed , Watershed management -- Zambezi River Watershed , Water resources development -- Zambezi River Watershed , Flood control -- Zambezi River Watershed , Flood forecasting -- Zambezi River Watershed , Rain gauges -- Zambezi River Watershed
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6054 , http://hdl.handle.net/10962/d1018915
- Description: The recent high profile flooding events – that have occurred in many parts of the world – have drawn attention to the need for new and improved methods for water resources assessment, water management and the modelling of large-scale flooding events. In the case of the Zambezi Basin, a review of the 2000 and 2001 floods identified the need for tools to enable hydrologists to assess and predict daily stream flow and identify the areas that are likely to be affected by flooding. As a way to address the problem, a methodology was set up to derive catchment soil moisture statistics from Earth Observation (EO) data and to study the improvements brought about by an assimilation of this information into hydrological models for improving reservoir management in a data scarce environment. Rainfall data were obtained from the FEWSNet Web site and computed by the National Oceanic and Atmospheric Administration Climatic Prediction Center (NOAA/CPC). These datasets were processed and used to monitor rainfall variability and subsequently fed into a hydrological model to predict the daily flows for the Zambezi River Basin. The hydrological model used was the Geospatial Stream Flow Model (GeoSFM), developed by the United States Geological Survey (USGS). GeoSFM is a spatially semi-distributed physically-based hydrological model, parameterised using spatially distributed topographic data, soil characteristics and land cover data sets available globally from both Remote Sensing and in situ sources. The Satellite rainfall data were validated against data from twenty (20) rainfall gauges located on the Lower Zambezi. However, at several rain gauge stations (especially those with complex topography, which tended to experience high rainfall spatial variability), there was no direct correlation between the satellite estimates and the ground data as recorded in daily time steps. The model was calibrated for seven gauging stations. The calibrated model performed quite well at seven selected locations (R2=0.66 to 0.90, CE=0.51 to 0.88, RSR=0.35 to 0.69, PBIAS=−4.5 to 7.5). The observed data were obtained from the National Water Agencies of the riparian countries. After GeoSFM calibration, the model generated an integration of the flows into a reservoir and hydropower model to optimise the operation of Kariba and Cahora Bassa dams. The Kariba and Cahora Bassa dams were selected because this study considers these two dams as the major infrastructures for controlling and alleviating floods in the Zambezi River Basin. Other dams (such as the Kafue and Itezhi-Thezi) were recognised in terms of their importance but including them was beyond the scope of this study because of financial and time constraints. The licence of the reservoir model was limited to one year for the same reason. The reservoir model used was the MIKE BASIN, a professional engineering software package and quasi-steady-state mass balance modelling tool for integrated river basin and management, developed by the Denmark Hydraulic Institute (DHI) in 2003. The model was parameterised by the geometry of the reservoir basin (level, area, volume relationships) and by the discharge-level (Q-h) relationship of the dam spillways. The integrated modelling system simulated the daily flow variation for all Zambezi River sub-basins between 1998 and 2008 and validated between 2009 and 2011. The resulting streamflows have been expressed in terms of hydrograph comparisons between simulated and observed flow values at the four gauging stations located downstream of Cahora Bassa dam. The integrated model performed well, between observed and forecast streamflows, at four selected gauging stations (R2=0.53 to 0.90, CE=0.50 to 0.80, RSR=0.49 to 0.69, PBIAS=−2.10 to 4.8). From the results of integrated modelling, it was observed that both Kariba and Cahora Bassa are currently being operated based on the maximum rule curve and both remain focused on maximising hydropower production and ensuring dam safety rather than other potential influences by the Zambezi River (such as flood control downstream – where the communities are located – and environmental issues). In addition, the flood mapping analysis demonstrated that the Cahora Bassa dam plays an important part in flood mitigation downstream of the dams. In the absence of optimisation of flow releases from both the Kariba and Cahora Bassa dams, in additional to the contribution of any other tributaries located downstream of the dams, the impact of flooding can be severe. As such, this study has developed new approaches for flood monitoring downstream of the Zambezi Basin, through the application of an integrated modelling system. The modelling system consists of: predicting daily streamflow (using the calibrated GeoSFM), then feeding the predicted streamflow into MIKE BASIN (for checking the operating rules) and to optimise the releases. Therefore, before releases are made, the flood maps can be used as a decision-making tool to both assess the impact of each level of release downstream and to identify the communities likely to be affected by the flood – this ensures that the necessary warnings can be issued before flooding occurs. Finally an integrated flood management tool was proposed – to host the results produced by the integrated system – which would then be accessible for assessment by the different users. These results were expressed in terms of water level (m). Four discharge-level (Q-h) relationships were developed for converting the simulated flow into water level at four selected sites downstream of Cahora Bassa dam – namely: Cahora Bassa dam site, Tete (E-320), Caia (E-291) and Marromeu (E-285). However, the uncertainties in these predictions suggested that improved monitoring systems may be achieved if data access at appropriate scale and quality was improved.
- Full Text:
- Authors: Vilanculos, Agostinho Chuquelane Fadulo
- Date: 2015
- Subjects: Hydrologic models -- Zambezi River Watershed , Watershed management -- Zambezi River Watershed , Water resources development -- Zambezi River Watershed , Flood control -- Zambezi River Watershed , Flood forecasting -- Zambezi River Watershed , Rain gauges -- Zambezi River Watershed
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6054 , http://hdl.handle.net/10962/d1018915
- Description: The recent high profile flooding events – that have occurred in many parts of the world – have drawn attention to the need for new and improved methods for water resources assessment, water management and the modelling of large-scale flooding events. In the case of the Zambezi Basin, a review of the 2000 and 2001 floods identified the need for tools to enable hydrologists to assess and predict daily stream flow and identify the areas that are likely to be affected by flooding. As a way to address the problem, a methodology was set up to derive catchment soil moisture statistics from Earth Observation (EO) data and to study the improvements brought about by an assimilation of this information into hydrological models for improving reservoir management in a data scarce environment. Rainfall data were obtained from the FEWSNet Web site and computed by the National Oceanic and Atmospheric Administration Climatic Prediction Center (NOAA/CPC). These datasets were processed and used to monitor rainfall variability and subsequently fed into a hydrological model to predict the daily flows for the Zambezi River Basin. The hydrological model used was the Geospatial Stream Flow Model (GeoSFM), developed by the United States Geological Survey (USGS). GeoSFM is a spatially semi-distributed physically-based hydrological model, parameterised using spatially distributed topographic data, soil characteristics and land cover data sets available globally from both Remote Sensing and in situ sources. The Satellite rainfall data were validated against data from twenty (20) rainfall gauges located on the Lower Zambezi. However, at several rain gauge stations (especially those with complex topography, which tended to experience high rainfall spatial variability), there was no direct correlation between the satellite estimates and the ground data as recorded in daily time steps. The model was calibrated for seven gauging stations. The calibrated model performed quite well at seven selected locations (R2=0.66 to 0.90, CE=0.51 to 0.88, RSR=0.35 to 0.69, PBIAS=−4.5 to 7.5). The observed data were obtained from the National Water Agencies of the riparian countries. After GeoSFM calibration, the model generated an integration of the flows into a reservoir and hydropower model to optimise the operation of Kariba and Cahora Bassa dams. The Kariba and Cahora Bassa dams were selected because this study considers these two dams as the major infrastructures for controlling and alleviating floods in the Zambezi River Basin. Other dams (such as the Kafue and Itezhi-Thezi) were recognised in terms of their importance but including them was beyond the scope of this study because of financial and time constraints. The licence of the reservoir model was limited to one year for the same reason. The reservoir model used was the MIKE BASIN, a professional engineering software package and quasi-steady-state mass balance modelling tool for integrated river basin and management, developed by the Denmark Hydraulic Institute (DHI) in 2003. The model was parameterised by the geometry of the reservoir basin (level, area, volume relationships) and by the discharge-level (Q-h) relationship of the dam spillways. The integrated modelling system simulated the daily flow variation for all Zambezi River sub-basins between 1998 and 2008 and validated between 2009 and 2011. The resulting streamflows have been expressed in terms of hydrograph comparisons between simulated and observed flow values at the four gauging stations located downstream of Cahora Bassa dam. The integrated model performed well, between observed and forecast streamflows, at four selected gauging stations (R2=0.53 to 0.90, CE=0.50 to 0.80, RSR=0.49 to 0.69, PBIAS=−2.10 to 4.8). From the results of integrated modelling, it was observed that both Kariba and Cahora Bassa are currently being operated based on the maximum rule curve and both remain focused on maximising hydropower production and ensuring dam safety rather than other potential influences by the Zambezi River (such as flood control downstream – where the communities are located – and environmental issues). In addition, the flood mapping analysis demonstrated that the Cahora Bassa dam plays an important part in flood mitigation downstream of the dams. In the absence of optimisation of flow releases from both the Kariba and Cahora Bassa dams, in additional to the contribution of any other tributaries located downstream of the dams, the impact of flooding can be severe. As such, this study has developed new approaches for flood monitoring downstream of the Zambezi Basin, through the application of an integrated modelling system. The modelling system consists of: predicting daily streamflow (using the calibrated GeoSFM), then feeding the predicted streamflow into MIKE BASIN (for checking the operating rules) and to optimise the releases. Therefore, before releases are made, the flood maps can be used as a decision-making tool to both assess the impact of each level of release downstream and to identify the communities likely to be affected by the flood – this ensures that the necessary warnings can be issued before flooding occurs. Finally an integrated flood management tool was proposed – to host the results produced by the integrated system – which would then be accessible for assessment by the different users. These results were expressed in terms of water level (m). Four discharge-level (Q-h) relationships were developed for converting the simulated flow into water level at four selected sites downstream of Cahora Bassa dam – namely: Cahora Bassa dam site, Tete (E-320), Caia (E-291) and Marromeu (E-285). However, the uncertainties in these predictions suggested that improved monitoring systems may be achieved if data access at appropriate scale and quality was improved.
- Full Text:
The value of locally isolated freshwater micro-algae in toxicity testing for water resource management in South Africa
- Authors: Gola, Nontutuzelo Pearl
- Date: 2015
- Subjects: Microalgae -- South Africa , Toxicity testing , Water resources development -- South Africa , Aquatic habitats -- South Africa , Water -- Pollution -- Toxicology , Water quality management -- South Africa , Sewage disposal in rivers, lakes, etc. -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6049 , http://hdl.handle.net/10962/d1017873
- Description: The ecological position of micro-algae at the base of the aquatic food web makes them critical components of aquatic ecosystems. Their short generation time also makes them useful biological indicators because they respond quickly to changes in environmental condition, enabling timely identification and assessment of water quality changes. The inclusion of micro-algae as indicators in water resource regulation and management in South Africa has started recently, their more extensive use in biomonitoring and ecotoxicology programmes for water resource management would contribute to the South African policy if water resource protection. The standard algal growth inhibition assay with the species Pseudokirchneriella subcapitata is currently used for monitoring toxicity of in-stream and industrial wastewater discharges to freshwater micro-algae. The relevance of the data generated by standard toxicity bioassays has been questioned, since micro-algae in particular are extremely variable in their sensitivity to a range of contaminants and these standard species used may not occur in the local aquatic environment. As a result, international regulatory agencies, have recommended algal growth inhibition tests be changed from a single standard species to tests with a number of species. One recommendation, in addition to the use of standard toxicity tests, is the use of species isolated from the local environment which may be more relevant for assessing site specific impacts. This study investigated the value and application of locally isolated South African freshwater micro-algae in toxicity tests for water resource management and was carried out in three phases. The first phase involved isolating micro-algae from South African aquatic resources. Micro-algae suitable for toxicity testing were identified and selected using as set of criteria. Three (Scenedesmus bicaudatus, Chlorella sorokiniana and Chlorella vulgaris) out of eight successfully isolated species satisfied the prescribed selection criteria and these were selected as potential toxicity test species. The second phase focused on refining and adapting the existing algal toxicity test protocol (the algal growth inhibition assay) for use on the locally isolated algal species. The refinement of the test protocol was achieved by exposing the locally isolated species to reference toxicants in order to assess and compare their growth and sensitivity to the toxicants under the prescribed toxicity test conditions with that of the standard toxicity test species (Pseudokirchneriella subcapitata) and a commercial laboratory species (Chlorella protothecoides). During this phase, one of the three local species (Scenedesmus bicaudatus) was eliminated as a potential toxicity test species due to inconsistent growth. The third phase of the study involved assessing the sensitivity of the two remaining species (C. vulgaris and C. sorokiniana) to a range of toxicants (reference toxicants, salts, effluents and a herbicide) and comparing it to that of the standard toxicity test species P. subcapitata and C. protothecoides. The toxicants were selected based on their relative importance in the South African context, as well as the practicality of using these local micro-algae to routinely determine the impact of these toxicants on local aquatic resources. The growth of the four micro-algae was stimulated by the selected effluents. The standard toxicity test species P. subcapitata was ranked the most sensitive and of the four species to two reference toxicants and two inorganic salts. Chlorella sorokiniana was ranked the most sensitive of the three Chlorella species to two reference toxicants and two inorganic salts. The herbicide stimulated the growth of C. vulgaris while inhibiting the growth of the other species. Pseudokirchneriela subcapitata and C. sorokiniana showed high intra-specific variability in growth, which made it difficult to determine the effective concentrations of the herbicide and therefore compare the sensitivity of the species. This varied response of micro-algal species to toxicants may result in the biodiversity shifts in aquatic ecosystems, and also supports the recommendation of using a battery of different species to support more informed decisions in water resource management.
- Full Text:
- Authors: Gola, Nontutuzelo Pearl
- Date: 2015
- Subjects: Microalgae -- South Africa , Toxicity testing , Water resources development -- South Africa , Aquatic habitats -- South Africa , Water -- Pollution -- Toxicology , Water quality management -- South Africa , Sewage disposal in rivers, lakes, etc. -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6049 , http://hdl.handle.net/10962/d1017873
- Description: The ecological position of micro-algae at the base of the aquatic food web makes them critical components of aquatic ecosystems. Their short generation time also makes them useful biological indicators because they respond quickly to changes in environmental condition, enabling timely identification and assessment of water quality changes. The inclusion of micro-algae as indicators in water resource regulation and management in South Africa has started recently, their more extensive use in biomonitoring and ecotoxicology programmes for water resource management would contribute to the South African policy if water resource protection. The standard algal growth inhibition assay with the species Pseudokirchneriella subcapitata is currently used for monitoring toxicity of in-stream and industrial wastewater discharges to freshwater micro-algae. The relevance of the data generated by standard toxicity bioassays has been questioned, since micro-algae in particular are extremely variable in their sensitivity to a range of contaminants and these standard species used may not occur in the local aquatic environment. As a result, international regulatory agencies, have recommended algal growth inhibition tests be changed from a single standard species to tests with a number of species. One recommendation, in addition to the use of standard toxicity tests, is the use of species isolated from the local environment which may be more relevant for assessing site specific impacts. This study investigated the value and application of locally isolated South African freshwater micro-algae in toxicity tests for water resource management and was carried out in three phases. The first phase involved isolating micro-algae from South African aquatic resources. Micro-algae suitable for toxicity testing were identified and selected using as set of criteria. Three (Scenedesmus bicaudatus, Chlorella sorokiniana and Chlorella vulgaris) out of eight successfully isolated species satisfied the prescribed selection criteria and these were selected as potential toxicity test species. The second phase focused on refining and adapting the existing algal toxicity test protocol (the algal growth inhibition assay) for use on the locally isolated algal species. The refinement of the test protocol was achieved by exposing the locally isolated species to reference toxicants in order to assess and compare their growth and sensitivity to the toxicants under the prescribed toxicity test conditions with that of the standard toxicity test species (Pseudokirchneriella subcapitata) and a commercial laboratory species (Chlorella protothecoides). During this phase, one of the three local species (Scenedesmus bicaudatus) was eliminated as a potential toxicity test species due to inconsistent growth. The third phase of the study involved assessing the sensitivity of the two remaining species (C. vulgaris and C. sorokiniana) to a range of toxicants (reference toxicants, salts, effluents and a herbicide) and comparing it to that of the standard toxicity test species P. subcapitata and C. protothecoides. The toxicants were selected based on their relative importance in the South African context, as well as the practicality of using these local micro-algae to routinely determine the impact of these toxicants on local aquatic resources. The growth of the four micro-algae was stimulated by the selected effluents. The standard toxicity test species P. subcapitata was ranked the most sensitive and of the four species to two reference toxicants and two inorganic salts. Chlorella sorokiniana was ranked the most sensitive of the three Chlorella species to two reference toxicants and two inorganic salts. The herbicide stimulated the growth of C. vulgaris while inhibiting the growth of the other species. Pseudokirchneriela subcapitata and C. sorokiniana showed high intra-specific variability in growth, which made it difficult to determine the effective concentrations of the herbicide and therefore compare the sensitivity of the species. This varied response of micro-algal species to toxicants may result in the biodiversity shifts in aquatic ecosystems, and also supports the recommendation of using a battery of different species to support more informed decisions in water resource management.
- Full Text:
Thermal physiology and behavioural ecology of the white shark, carcharodon carcharias
- Authors: Gennari, Enrico
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64002 , vital:28522
- Description: Expected release date-May 2019
- Full Text:
- Authors: Gennari, Enrico
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64002 , vital:28522
- Description: Expected release date-May 2019
- Full Text:
Towards a Mobile Bioethanol Unit for point of source conversion of sugar sources to bioethanol: design and feasibility study for South Africa
- Authors: Cech, Alexandra Louise
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/59141 , vital:27439
- Description: Restricted access-thesis embargoed for 5 years
- Full Text:
- Authors: Cech, Alexandra Louise
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/59141 , vital:27439
- Description: Restricted access-thesis embargoed for 5 years
- Full Text:
Transnational habitus : Mariem Hassan as the transcultural representation of the relationship between Saharaui music and Nubenegra records
- Authors: Gimenez Amoros, Luis
- Date: 2015
- Subjects: Hassan, Mariem, 1958- , Nubenegra Records , Sahrawi (African people) -- Music -- Cross-cultural studies , Sahrawi (African people) -- Music -- Foreign influences , Transnationalism , Cross-cultural studies -- Spain
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2697 , http://hdl.handle.net/10962/d1017819
- Description: This thesis expands on primary field research conducted for my MMus degree. Undertaken in the Saharaui refugee camps of southern Algeria (2004-2005) that research - based on ethnographic data and the analysis of Saharaui music, known as Haul ¹- focussed on the musical system, the social context of musical performance and the music culture in Saharaui refugee camps. This doctoral research examines Saharaui Haul music as practised in Spain and is particularly focussed on its entry, since 1998, into the global market by way of the World Music label, Nubenegra records. The encounter between Saharaui musicians and Nubenegra records has created a new type of Saharaui Haul which is different to that played in the refugee camps. This phenomenon has emerged as a result of western music producers compelling Saharaui musicians to introduce musical changes so that both parties may be considered as musical agents occupying different positions on a continuum of tradition and change. Nubenegra undertook the commodification of Saharaui music and disseminated it from the camps to the rest of the world. A musical and social analysis of the relationship between Nubenegra and Saharaui musicians living in Spain will form the basis of the research in this thesis. In particular, Mariem Hassan is an example of a musician who had her music disseminated through the relationship with Nubenegra and she is promoted as the music ambassador of the Western Sahara. I collaborated with her as a composer and performer on her last album, El Aaiun egdat (Aaiun in fire), in 2012² and gained first hand insight into the relationship between Mariem and Nubenegra. This thesis reflects on this relationship and my role in facilitating this encounter.
- Full Text:
- Authors: Gimenez Amoros, Luis
- Date: 2015
- Subjects: Hassan, Mariem, 1958- , Nubenegra Records , Sahrawi (African people) -- Music -- Cross-cultural studies , Sahrawi (African people) -- Music -- Foreign influences , Transnationalism , Cross-cultural studies -- Spain
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2697 , http://hdl.handle.net/10962/d1017819
- Description: This thesis expands on primary field research conducted for my MMus degree. Undertaken in the Saharaui refugee camps of southern Algeria (2004-2005) that research - based on ethnographic data and the analysis of Saharaui music, known as Haul ¹- focussed on the musical system, the social context of musical performance and the music culture in Saharaui refugee camps. This doctoral research examines Saharaui Haul music as practised in Spain and is particularly focussed on its entry, since 1998, into the global market by way of the World Music label, Nubenegra records. The encounter between Saharaui musicians and Nubenegra records has created a new type of Saharaui Haul which is different to that played in the refugee camps. This phenomenon has emerged as a result of western music producers compelling Saharaui musicians to introduce musical changes so that both parties may be considered as musical agents occupying different positions on a continuum of tradition and change. Nubenegra undertook the commodification of Saharaui music and disseminated it from the camps to the rest of the world. A musical and social analysis of the relationship between Nubenegra and Saharaui musicians living in Spain will form the basis of the research in this thesis. In particular, Mariem Hassan is an example of a musician who had her music disseminated through the relationship with Nubenegra and she is promoted as the music ambassador of the Western Sahara. I collaborated with her as a composer and performer on her last album, El Aaiun egdat (Aaiun in fire), in 2012² and gained first hand insight into the relationship between Mariem and Nubenegra. This thesis reflects on this relationship and my role in facilitating this encounter.
- Full Text:
Uncertainties in modelling hydrological responses in gauged and ungauged sub‐basins
- Authors: Tumbo, Madaka Harold
- Date: 2015
- Subjects: Hydrologic models , Watersheds -- Tanzania , Water-supply -- Tanzania -- Great Ruaha River Watershed , Water resources development -- Tanzania -- Great Ruaha River Watershed , Rain and rainfall -- Mathematical models , Rain gauges -- Tanzania -- Great Ruaha River Watershed , Great Ruaha River Watershed (Tanzania)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6053 , http://hdl.handle.net/10962/d1018568
- Description: The world is undergoing rapid changes and the future is uncertain. The changes are related to modification of the landscape due to human activities, such as large and small scale irrigation, afforestation and changes to the climate system. Understanding and predicting hydrologic change is one of the challenges facing hydrologists today. Part of this understanding can be developed from observed data, however, there often too few observations and those that are available are frequently affected by uncertainties. Hydrological models have become essential tools for understanding historical variations of catchment hydrology and for predicting future possible trends. However, most developing countries are faced with poor spatial distributions of rainfall and evaporation stations that provide the data used to force models, as well as stream flow gauging stations to provide the data for establishing models and for evaluating their success. Hydrological models are faced with a number of challenges which include poor input data (data quality and poorly quantified human activities on observed stream flow data), uncertainties associated with model complexity and structure, the methods used to quantify model parameters, together with the difficulties of understanding hydrological processes at the catchment or subbasin. Within hydrological modelling, there is currently a trend of dealing with equifinality through the evaluation of parameter identifiability and the quantification of uncertainty bands associated with the predictions of the model. Hydrological models should not only focus on reproducing the past behaviour of a basin, but also on evaluating the representativeness of the surface and subsurface model components and their ability to simulate reality for the correct reasons. Part of this modelling process therefore involves quantifying and including all the possible sources of uncertainty. Uncertainty analysis has become the standard approach to most hydrological modelling studies, but has yet to be effectively used in practical water resources assessment. This study applied a hydrological modelling approach for understanding the hydrology of a large Tanzanian drainage basin, the Great Ruaha River that has many areas that are ungauged and where the available data (climate, stream flow and existing water use) are subject to varying degrees of uncertainty. The Great Ruaha River (GRR) is an upstream tributary of the Rufiji River Basin within Tanzania and covers an area of 86 000 km2. The basin is drained by four main tributaries; the Upper Great Ruaha, the Kisigo, the Little Ruaha and the Lukosi. The majority of the runoff is generated from the Chunya escarpment, the Kipengere ranges and the Poroto Mountains. The runoff generated feeds the alluvial and seasonally flooded Usangu plains (including the Ihefu perennial swamp). The majority of the irrigation water use in the basin is located where headwater sub‐basins drain towards the Usangu plains. The overall objective was to establish uncertain but behavioural hydrological models that could be useful for future water resources assessments that are likely to include issues of land use change, changes in patterns of abstraction and water use, as well the possibility of change in future climates.
- Full Text:
- Authors: Tumbo, Madaka Harold
- Date: 2015
- Subjects: Hydrologic models , Watersheds -- Tanzania , Water-supply -- Tanzania -- Great Ruaha River Watershed , Water resources development -- Tanzania -- Great Ruaha River Watershed , Rain and rainfall -- Mathematical models , Rain gauges -- Tanzania -- Great Ruaha River Watershed , Great Ruaha River Watershed (Tanzania)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6053 , http://hdl.handle.net/10962/d1018568
- Description: The world is undergoing rapid changes and the future is uncertain. The changes are related to modification of the landscape due to human activities, such as large and small scale irrigation, afforestation and changes to the climate system. Understanding and predicting hydrologic change is one of the challenges facing hydrologists today. Part of this understanding can be developed from observed data, however, there often too few observations and those that are available are frequently affected by uncertainties. Hydrological models have become essential tools for understanding historical variations of catchment hydrology and for predicting future possible trends. However, most developing countries are faced with poor spatial distributions of rainfall and evaporation stations that provide the data used to force models, as well as stream flow gauging stations to provide the data for establishing models and for evaluating their success. Hydrological models are faced with a number of challenges which include poor input data (data quality and poorly quantified human activities on observed stream flow data), uncertainties associated with model complexity and structure, the methods used to quantify model parameters, together with the difficulties of understanding hydrological processes at the catchment or subbasin. Within hydrological modelling, there is currently a trend of dealing with equifinality through the evaluation of parameter identifiability and the quantification of uncertainty bands associated with the predictions of the model. Hydrological models should not only focus on reproducing the past behaviour of a basin, but also on evaluating the representativeness of the surface and subsurface model components and their ability to simulate reality for the correct reasons. Part of this modelling process therefore involves quantifying and including all the possible sources of uncertainty. Uncertainty analysis has become the standard approach to most hydrological modelling studies, but has yet to be effectively used in practical water resources assessment. This study applied a hydrological modelling approach for understanding the hydrology of a large Tanzanian drainage basin, the Great Ruaha River that has many areas that are ungauged and where the available data (climate, stream flow and existing water use) are subject to varying degrees of uncertainty. The Great Ruaha River (GRR) is an upstream tributary of the Rufiji River Basin within Tanzania and covers an area of 86 000 km2. The basin is drained by four main tributaries; the Upper Great Ruaha, the Kisigo, the Little Ruaha and the Lukosi. The majority of the runoff is generated from the Chunya escarpment, the Kipengere ranges and the Poroto Mountains. The runoff generated feeds the alluvial and seasonally flooded Usangu plains (including the Ihefu perennial swamp). The majority of the irrigation water use in the basin is located where headwater sub‐basins drain towards the Usangu plains. The overall objective was to establish uncertain but behavioural hydrological models that could be useful for future water resources assessments that are likely to include issues of land use change, changes in patterns of abstraction and water use, as well the possibility of change in future climates.
- Full Text:
Understanding climate variability and livelihoods adaptation in rural Zimbabwe : case of Charewa, Mutoko
- Authors: Bhatasara, Sandra
- Date: 2015
- Subjects: Climatic changes -- Zimbabwe -- Mutoko , Sustainable agriculture -- Zimbabwe -- Mutoko , Environmental impact analysis -- Zimbabwe -- Mutoko , Farmers -- Zimbabwe -- Mutoko -- Economic conditions , Food supply -- Zimbabwe -- Mutoko , Farms -- Zimbabwe -- Mutoko , Land use, Rural -- Zimbabwe -- Mutoko , Crops and climate -- Zimbabwe -- Mutoko
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3403 , http://hdl.handle.net/10962/d1018928 , Climatic changes -- Zimbabwe -- Mutoko , Sustainable agriculture -- Zimbabwe -- Mutoko , Environmental impact analysis -- Zimbabwe -- Mutoko , Farmers -- Zimbabwe -- Mutoko -- Economic conditions , Food supply -- Zimbabwe -- Mutoko , Farms -- Zimbabwe -- Mutoko , Land use, Rural -- Zimbabwe -- Mutoko , Crops and climate -- Zimbabwe -- Mutoko
- Description: Rural farmers in Zimbabwe have been grappling with various changes and challenges occurring in the country since the early 1990s. Amongst these, climate variability has emerged as one significant aspect. It has introduced new challenges for these farmers who are already facing various difficulties in maintaining their insecure livelihoods. Yet, current adaptation theories and inquiries have failed to sufficiently account for and analyse the capacity of these farmers to adequately respond to changing climatic conditions. In this respect, a number of studies have been heavily embedded in deterministic concepts that regard rural farmers as passive victims who play only a minor part in decisions and actions that affect their own livelihoods and well-being. Similarly, although some studies have acknowledged farmers’ capacity to adapt and build elements of resilience, they have not adequately shown how farmers interpret changes in climate and the structures, processes and conditions underpinning adaptation. Following that, my study uses a case study of a rural community in a semi-arid region of Mutoko district in eastern Zimbabwe and Margaret Archer’s sociological theory to understand and analyse how farmers problematise climate variability and respond to it. The study utilises a qualitative approach to divulge the subtleties on how rural people interpret processes of change and adapt to such changes. The thesis found that farmers are encountering increasingly unpredictable and unreliable rainfall patterns as well as shifting temperature conditions which are inducing labyrinthian livelihoods conundrums. However, these climatic shifts are not being experienced in a discrete manner hence farmers are also discontented with the obtaining socio-economic circumstances in the country. Simultaneously, whilst farmers in large part conceived changes in rainfall and temperature to be caused by natural shifts in climate, they also ascribed them to cultural and religious facets. Importantly, the thesis reveals considerable resourcefulness by farmers in the face of nascent changes in climate variability. Farmers have therefore constructed versatile coping and adaptive strategies. What is crucial to mention here is that climatic and non-climatic challenges are negotiated concurrently. Therein, farmers are adapting to climate variability and at the same time navigating difficult socio-economic landscapes. All the same, the process of adaptation is ostensibly not straightforward but complex. As it evolves, farmers find themselves facing numerous constraining structures and processes. Nonetheless, farmers in this study are able to circumvent the constraints presented to them and at the same time activate the corresponding enabling structures, processes and conditions.
- Full Text:
- Authors: Bhatasara, Sandra
- Date: 2015
- Subjects: Climatic changes -- Zimbabwe -- Mutoko , Sustainable agriculture -- Zimbabwe -- Mutoko , Environmental impact analysis -- Zimbabwe -- Mutoko , Farmers -- Zimbabwe -- Mutoko -- Economic conditions , Food supply -- Zimbabwe -- Mutoko , Farms -- Zimbabwe -- Mutoko , Land use, Rural -- Zimbabwe -- Mutoko , Crops and climate -- Zimbabwe -- Mutoko
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3403 , http://hdl.handle.net/10962/d1018928 , Climatic changes -- Zimbabwe -- Mutoko , Sustainable agriculture -- Zimbabwe -- Mutoko , Environmental impact analysis -- Zimbabwe -- Mutoko , Farmers -- Zimbabwe -- Mutoko -- Economic conditions , Food supply -- Zimbabwe -- Mutoko , Farms -- Zimbabwe -- Mutoko , Land use, Rural -- Zimbabwe -- Mutoko , Crops and climate -- Zimbabwe -- Mutoko
- Description: Rural farmers in Zimbabwe have been grappling with various changes and challenges occurring in the country since the early 1990s. Amongst these, climate variability has emerged as one significant aspect. It has introduced new challenges for these farmers who are already facing various difficulties in maintaining their insecure livelihoods. Yet, current adaptation theories and inquiries have failed to sufficiently account for and analyse the capacity of these farmers to adequately respond to changing climatic conditions. In this respect, a number of studies have been heavily embedded in deterministic concepts that regard rural farmers as passive victims who play only a minor part in decisions and actions that affect their own livelihoods and well-being. Similarly, although some studies have acknowledged farmers’ capacity to adapt and build elements of resilience, they have not adequately shown how farmers interpret changes in climate and the structures, processes and conditions underpinning adaptation. Following that, my study uses a case study of a rural community in a semi-arid region of Mutoko district in eastern Zimbabwe and Margaret Archer’s sociological theory to understand and analyse how farmers problematise climate variability and respond to it. The study utilises a qualitative approach to divulge the subtleties on how rural people interpret processes of change and adapt to such changes. The thesis found that farmers are encountering increasingly unpredictable and unreliable rainfall patterns as well as shifting temperature conditions which are inducing labyrinthian livelihoods conundrums. However, these climatic shifts are not being experienced in a discrete manner hence farmers are also discontented with the obtaining socio-economic circumstances in the country. Simultaneously, whilst farmers in large part conceived changes in rainfall and temperature to be caused by natural shifts in climate, they also ascribed them to cultural and religious facets. Importantly, the thesis reveals considerable resourcefulness by farmers in the face of nascent changes in climate variability. Farmers have therefore constructed versatile coping and adaptive strategies. What is crucial to mention here is that climatic and non-climatic challenges are negotiated concurrently. Therein, farmers are adapting to climate variability and at the same time navigating difficult socio-economic landscapes. All the same, the process of adaptation is ostensibly not straightforward but complex. As it evolves, farmers find themselves facing numerous constraining structures and processes. Nonetheless, farmers in this study are able to circumvent the constraints presented to them and at the same time activate the corresponding enabling structures, processes and conditions.
- Full Text:
Water resources availability in the Caledon River basin : past, present and future
- Authors: Mohobane, Thabiso
- Date: 2015
- Subjects: Water-supply -- South Africa -- Caledon River Valley , Climatic changes -- South Africa -- Caledon River Valley , Hydrologic models -- South Africa -- Caledon River Valley , Precipitation forecasting -- South Africa -- Caledon River Valley , Water-supply -- Forecasting , Runoff -- Mathematical models , Evapotranspiration -- South Africa -- Caledon River Valley
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6055 , http://hdl.handle.net/10962/d1019802
- Description: The Caledon River Basin is located on one of the most water-scarce region on the African continent. The water resources of the Caledon River Basin play a pivotal role in socio-economic activities in both Lesotho and South Africa but the basin experiences recurrent severe droughts and frequent water shortages. The Caledon River is mostly used for commercial and subsistence agriculture, industrial and domestic supply. The resources are also important beyond the basin’s boundaries as the water is transferred to the nearby Modder River. The Caledon River is also a significant tributary to the Orange-Senqu Basin, which is shared by five southern African countries. However, the water resources in the basin are under continuous threat as a result of rapidly growing population, economic growth as well as changing climate, amongst others. It is therefore important that the hydrological regime and water resources of the basin are thoroughly evaluated and assessed so that they can be sustainably managed and utilised for maximum economic benefits. Climate change has been identified by the international community as one of the most prominent threats to peace, food security and livelihood and southern Africa as among the most vulnerable regions of the world. Water resources are perceived as a natural resource which will be affected the most by the changing climate conditions. Global warming is expected to bring more severe, prolonged droughts and exacerbate water shortages in this region. The current study is mainly focused on investigating the impacts of climate change on the water resources of the Caledon River Basin. The main objectives of the current study included assessing the past and current hydrological characteristics of the Caledon River Basin under current state of the physical environment, observed climate conditions and estimated water use; detecting any changes in the future rainfall and evaporative demands relative to present conditions and evaluating the impacts of climate on the basin’s hydrological regime and water resources availability for the future climate scenario, 2046-2065. To achieve these objectives the study used observed hydrological, meteorological data sets and the basin’s physical characteristics to establish parameters of the Pitman and WEAP hydrological models. Hydrological modelling is an integral part of hydrological investigations and evaluations. The various sources of uncertainties in the outputs of the climate and hydrological models were identified and quantified, as an integral part of the whole exercise. The 2-step approach of the uncertainty version of the model was used to estimate a range of parameters yielding behavioural natural flow ensembles. This approach uses the regional and local hydrological signals to constrain the model parameter ranges. The estimated parameters were also employed to guide the calibration process of the Water Evaluation And Planning (WEAP) model. The two models incorporated the estimated water uses within the basin to establish the present day flow simulations and they were found to sufficiently simulate the present day flows, as compared to the observed flows. There is an indication therefore, that WEAP can be successfully applied in other regions for hydrological investigations. Possible changes in future climate regime of the basin were evaluated by analysing downscaled temperature and rainfall outputs from a set of 9 climate models. The predictions are based on the A2 greenhouse gases emission scenario which assumes a continuous increase in emission rates. While the climate models agree that temperature, and hence, evapotranspiration will increase in the future, they demonstrate significant disagreement on whether rainfall will decrease or increase and by how much. The disagreement of the GCMs on projected future rainfall constitutes a major uncertainty in the prediction of water resources availability of the basin. This is to the extent that according to 7 out of 9 climate models used, the stream flow in four sub-basins (D21E, D22B, D23D and D23F) in the Caledon River Basin is projected to decrease below the present day flows, while two models (IPSL and MIUB) consistently project enhanced water resource availability in the basin in the future. The differences in the GCM projections highlight the margin of uncertainty involved predicting the future status of water resources in the basin. Such uncertainty should not be ignored and these results can be useful in aiding decision-makers to develop policies that are robust and that encompass all possibilities. In an attempt to reduce the known uncertainties, the study recommends upgrading of the hydrological monitoring network within the Caledon River Basin to facilitate improved hydrological evaluation and management. It also suggests the use of updated climate change data from the newest generation climate models, as well as integrating the findings of the current research into water resources decision making process.
- Full Text:
- Authors: Mohobane, Thabiso
- Date: 2015
- Subjects: Water-supply -- South Africa -- Caledon River Valley , Climatic changes -- South Africa -- Caledon River Valley , Hydrologic models -- South Africa -- Caledon River Valley , Precipitation forecasting -- South Africa -- Caledon River Valley , Water-supply -- Forecasting , Runoff -- Mathematical models , Evapotranspiration -- South Africa -- Caledon River Valley
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6055 , http://hdl.handle.net/10962/d1019802
- Description: The Caledon River Basin is located on one of the most water-scarce region on the African continent. The water resources of the Caledon River Basin play a pivotal role in socio-economic activities in both Lesotho and South Africa but the basin experiences recurrent severe droughts and frequent water shortages. The Caledon River is mostly used for commercial and subsistence agriculture, industrial and domestic supply. The resources are also important beyond the basin’s boundaries as the water is transferred to the nearby Modder River. The Caledon River is also a significant tributary to the Orange-Senqu Basin, which is shared by five southern African countries. However, the water resources in the basin are under continuous threat as a result of rapidly growing population, economic growth as well as changing climate, amongst others. It is therefore important that the hydrological regime and water resources of the basin are thoroughly evaluated and assessed so that they can be sustainably managed and utilised for maximum economic benefits. Climate change has been identified by the international community as one of the most prominent threats to peace, food security and livelihood and southern Africa as among the most vulnerable regions of the world. Water resources are perceived as a natural resource which will be affected the most by the changing climate conditions. Global warming is expected to bring more severe, prolonged droughts and exacerbate water shortages in this region. The current study is mainly focused on investigating the impacts of climate change on the water resources of the Caledon River Basin. The main objectives of the current study included assessing the past and current hydrological characteristics of the Caledon River Basin under current state of the physical environment, observed climate conditions and estimated water use; detecting any changes in the future rainfall and evaporative demands relative to present conditions and evaluating the impacts of climate on the basin’s hydrological regime and water resources availability for the future climate scenario, 2046-2065. To achieve these objectives the study used observed hydrological, meteorological data sets and the basin’s physical characteristics to establish parameters of the Pitman and WEAP hydrological models. Hydrological modelling is an integral part of hydrological investigations and evaluations. The various sources of uncertainties in the outputs of the climate and hydrological models were identified and quantified, as an integral part of the whole exercise. The 2-step approach of the uncertainty version of the model was used to estimate a range of parameters yielding behavioural natural flow ensembles. This approach uses the regional and local hydrological signals to constrain the model parameter ranges. The estimated parameters were also employed to guide the calibration process of the Water Evaluation And Planning (WEAP) model. The two models incorporated the estimated water uses within the basin to establish the present day flow simulations and they were found to sufficiently simulate the present day flows, as compared to the observed flows. There is an indication therefore, that WEAP can be successfully applied in other regions for hydrological investigations. Possible changes in future climate regime of the basin were evaluated by analysing downscaled temperature and rainfall outputs from a set of 9 climate models. The predictions are based on the A2 greenhouse gases emission scenario which assumes a continuous increase in emission rates. While the climate models agree that temperature, and hence, evapotranspiration will increase in the future, they demonstrate significant disagreement on whether rainfall will decrease or increase and by how much. The disagreement of the GCMs on projected future rainfall constitutes a major uncertainty in the prediction of water resources availability of the basin. This is to the extent that according to 7 out of 9 climate models used, the stream flow in four sub-basins (D21E, D22B, D23D and D23F) in the Caledon River Basin is projected to decrease below the present day flows, while two models (IPSL and MIUB) consistently project enhanced water resource availability in the basin in the future. The differences in the GCM projections highlight the margin of uncertainty involved predicting the future status of water resources in the basin. Such uncertainty should not be ignored and these results can be useful in aiding decision-makers to develop policies that are robust and that encompass all possibilities. In an attempt to reduce the known uncertainties, the study recommends upgrading of the hydrological monitoring network within the Caledon River Basin to facilitate improved hydrological evaluation and management. It also suggests the use of updated climate change data from the newest generation climate models, as well as integrating the findings of the current research into water resources decision making process.
- Full Text:
"I felt that I deserved it" : an Investigation into HIV-related PTSD, traumatic life events, and the personal experiences of living with HIV : a mixed-method study
- Authors: Boulind, Melissa Jane
- Date: 2014
- Subjects: Post-traumatic stress disorder -- Psychological aspects -- Research -- South Africa -- Kwazulu HIV infections -- Psychological aspects -- Research -- South Africa -- Kwazulu HIV-positive persons -- South Africa -- Kwazulu -- Interviews Psychic trauma -- Research Stress (Psychology) -- Research
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3211 , http://hdl.handle.net/10962/d1012172
- Description: There appears to be a growing body of literature focusing on PTSD and HIV-related PTSD (the diagnosis of HIV being the significant traumatic event) amongst HIV-positive samples, but only a few African studies that attempt to estimate the prevalence of PTSD amongst HIV-positive people, and even fewer that attempt to estimate the prevalence of HIV-related PTSD. The systemic review presented in this study is currently fully inclusive and is the most up-to-date available. Estimates of the prevalence of PTSD and HIV-related PTSD in South Africa range from 0.7 to 54.1% and, 4.2 to 40% respectively. The current cross-sectional study made use of a mixed-method approach to investigate traumatic life events, PTSD and HIV-related PTSD within a primary health-care centre in KwaZulu-Natal. The quantitative sample consisted of 159 adults (18-50 years) who were compliant on ARV medication. Using the CIDI-PTSD module, the adapted CIDI-PTSD module for HIV, and IES-R, findings indicated that 62% had reported some kind of traumatic event in their lifetime, with 29.6% of participants meeting the criteria for lifetime PTSD, and 40.9% meeting the criteria for lifetime HIV-related PTSD. Altogether, 57.9% of individuals met the criteria for some form of PTSD (either regular PTSD or HIV-related PTSD), and 12.6% met the criteria for both PTSD and HIV-related PTSD. Of the different categories of traumatic events, interpersonal violence has the highest rate of PTSD, followed by a diagnosis of and living with HIV, and then disaster. Furthermore, the IES-R was compared for its usefulness as a screening measure for PTSD against both the CIDI, but results suggest that it is an inferior screening measure to the PDS. The qualitative study consisted of six participants who were examined using IPA methodology informed by the Ehlers and Clark (2000) Model of trauma. Their experiences revealed experiences of stigma, a number of negative appraisals, negative emotions and coping behaviours. Some of the latter might serve as compensatory mechanisms to avoid negative judgements. Hypervigilance seems to be a feature of ARV-compliance that might confer added vulnerability to PTSD and other anxiety disorders.
- Full Text:
- Authors: Boulind, Melissa Jane
- Date: 2014
- Subjects: Post-traumatic stress disorder -- Psychological aspects -- Research -- South Africa -- Kwazulu HIV infections -- Psychological aspects -- Research -- South Africa -- Kwazulu HIV-positive persons -- South Africa -- Kwazulu -- Interviews Psychic trauma -- Research Stress (Psychology) -- Research
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3211 , http://hdl.handle.net/10962/d1012172
- Description: There appears to be a growing body of literature focusing on PTSD and HIV-related PTSD (the diagnosis of HIV being the significant traumatic event) amongst HIV-positive samples, but only a few African studies that attempt to estimate the prevalence of PTSD amongst HIV-positive people, and even fewer that attempt to estimate the prevalence of HIV-related PTSD. The systemic review presented in this study is currently fully inclusive and is the most up-to-date available. Estimates of the prevalence of PTSD and HIV-related PTSD in South Africa range from 0.7 to 54.1% and, 4.2 to 40% respectively. The current cross-sectional study made use of a mixed-method approach to investigate traumatic life events, PTSD and HIV-related PTSD within a primary health-care centre in KwaZulu-Natal. The quantitative sample consisted of 159 adults (18-50 years) who were compliant on ARV medication. Using the CIDI-PTSD module, the adapted CIDI-PTSD module for HIV, and IES-R, findings indicated that 62% had reported some kind of traumatic event in their lifetime, with 29.6% of participants meeting the criteria for lifetime PTSD, and 40.9% meeting the criteria for lifetime HIV-related PTSD. Altogether, 57.9% of individuals met the criteria for some form of PTSD (either regular PTSD or HIV-related PTSD), and 12.6% met the criteria for both PTSD and HIV-related PTSD. Of the different categories of traumatic events, interpersonal violence has the highest rate of PTSD, followed by a diagnosis of and living with HIV, and then disaster. Furthermore, the IES-R was compared for its usefulness as a screening measure for PTSD against both the CIDI, but results suggest that it is an inferior screening measure to the PDS. The qualitative study consisted of six participants who were examined using IPA methodology informed by the Ehlers and Clark (2000) Model of trauma. Their experiences revealed experiences of stigma, a number of negative appraisals, negative emotions and coping behaviours. Some of the latter might serve as compensatory mechanisms to avoid negative judgements. Hypervigilance seems to be a feature of ARV-compliance that might confer added vulnerability to PTSD and other anxiety disorders.
- Full Text:
(Re-)inventing our selves/ourselves : identity and community in contemporary South African short fiction cycles.
- Authors: Marais, Susan Jacqueline
- Date: 2014
- Subjects: South African fiction (English) -- History and criticism , Matlou, Joël -- Criticism and interpretation , Magona, Sindiwe -- Criticism and interpretation , Vladislavić, Ivan, 1957- -- Criticism and interpretation , Wicomb, Zoë -- Criticism and interpretation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2326 , http://hdl.handle.net/10962/d1016357
- Description: In this study I focus on a number of collections of short fiction by the South African writers Joël Matlou, Sindiwe Magona, Zoë Wicomb and Ivan Vladislavić, all of which evince certain of the characteristics of short story cycles or sequences. In other words, they display what Forrest L. Ingram describes as “a double tendency of asserting the individuality of [their] components on the one hand and of highlighting, on the other, the bonds of unity which make the many into a single whole”. The cycle form, thus defined, is characterised by a paradoxical yet productive and frequently unresolved tension between “the individuality of each of the stories and the necessities of the larger unit”, between “the one and the many”, and between cohesion and fragmentation. It is this “dynamic structure of connection and disconnection” which singularly equips the genre to represent the interrelationship of singular and collective identities, or the “coherent multiplicity of community”. Ingram, for example, asserts that “Numerous and varied connective strands draw the co-protagonists of any story cycle into a single community. … However this community may be achieved, it usually can be said to constitute the central character of a cycle”. Not unsurprisingly, then, in its dominant manifestations over much of the twentieth century the short story cycle demonstrated a marked inclination towards regionalism and the depiction of localised enclaves, and this tendency towards “place-based short story cycles” in which topographical unity is a conspicuous feature was as pronounced in South Africa as elsewhere. However, the specific collections which are my concern here increasingly employ innovative and self-reflexive narrative strategies that unsettle generic expectations and interrogate the notions of regionalism and community conventionally associated with the short story cycle. My investigation seeks to explain this shift in emphasis, and its particular significance within the South African context.
- Full Text:
- Authors: Marais, Susan Jacqueline
- Date: 2014
- Subjects: South African fiction (English) -- History and criticism , Matlou, Joël -- Criticism and interpretation , Magona, Sindiwe -- Criticism and interpretation , Vladislavić, Ivan, 1957- -- Criticism and interpretation , Wicomb, Zoë -- Criticism and interpretation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2326 , http://hdl.handle.net/10962/d1016357
- Description: In this study I focus on a number of collections of short fiction by the South African writers Joël Matlou, Sindiwe Magona, Zoë Wicomb and Ivan Vladislavić, all of which evince certain of the characteristics of short story cycles or sequences. In other words, they display what Forrest L. Ingram describes as “a double tendency of asserting the individuality of [their] components on the one hand and of highlighting, on the other, the bonds of unity which make the many into a single whole”. The cycle form, thus defined, is characterised by a paradoxical yet productive and frequently unresolved tension between “the individuality of each of the stories and the necessities of the larger unit”, between “the one and the many”, and between cohesion and fragmentation. It is this “dynamic structure of connection and disconnection” which singularly equips the genre to represent the interrelationship of singular and collective identities, or the “coherent multiplicity of community”. Ingram, for example, asserts that “Numerous and varied connective strands draw the co-protagonists of any story cycle into a single community. … However this community may be achieved, it usually can be said to constitute the central character of a cycle”. Not unsurprisingly, then, in its dominant manifestations over much of the twentieth century the short story cycle demonstrated a marked inclination towards regionalism and the depiction of localised enclaves, and this tendency towards “place-based short story cycles” in which topographical unity is a conspicuous feature was as pronounced in South Africa as elsewhere. However, the specific collections which are my concern here increasingly employ innovative and self-reflexive narrative strategies that unsettle generic expectations and interrogate the notions of regionalism and community conventionally associated with the short story cycle. My investigation seeks to explain this shift in emphasis, and its particular significance within the South African context.
- Full Text:
A case study of a pre-service mathematics education course to grow and develop proficient teaching in mathematics in the intermediate phase
- Authors: Lee, Amanda Jane
- Date: 2014
- Subjects: Mathematics -- Study and teaching -- South Africa , Mathematics teachers -- Training of -- South Africa , Mathematics -- Study and teaching (Elementary) -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2004 , http://hdl.handle.net/10962/d1015664
- Description: This research study investigated the ways in which a mathematics module, informed by an enactivist philosophy, enabled pre-service teachers to unpack the reality of their teaching practice in terms of proficient teaching. Given the generally poor mathematics results in South Africa it is not enough for teachers to be merely proficient in Mathematics. They also need to be in a position to explain important mathematical concepts to children in a manner that will encourage and develop an understanding of the basic mathematical concepts. It was my intention with this study to determine whether a mathematics education module, that embraced the underlying themes of enactivism as part of its teaching pedagogy, could have the potential to develop and increase the skills of pre-service teachers’ teaching for proficiency in Mathematics. The mathematics module was underpinned by five themes of enactivism namely: autonomy, embodiment, emergence, sense-making and experience and was designed to supplement the pre-service teachers’ basic skills in Mathematics in the Intermediate Phase. This mathematics module was offered to fourth year pre-service teachers completing a B.Ed. in the Foundation Phase at a private institute specialising in the training of teachers. The theoretical framework was informed by enactivism and how the themes of enactivism could be used as a vehicle to develop teaching proficiency. The study was qualitative in nature and situated within an interpretivist paradigm. The specific perspectives of interpretivism that were used were hermeneutics, phenomenology and reflexivity. The research design was a case study that contained elements of action research and encompassed three phases of data collection. The first phase focused on the pre-service teachers’ approach to teaching Mathematics and what this brought forth in terms of the reality of their teaching practice and the problems they encountered. The second phase undertook to determine what growth and development of teaching proficiency in Mathematics had emerged over the research period. The final phase was undertaken after the pre-service teachers had graduated and were employed as full time teachers in the Intermediate Phase. The analytical framework and lens through which the data was analysed was that of Kilpatrick, Swafford and Findell’s (2001) strands of mathematical proficiency. The argument that I present is that the themes of enactivism did contribute to the growth of the pre-service teachers’ teaching for mathematical proficiency. The themes of embodiment and experience were major contributions in revealing that this was a reality for the pre-service teachers from a practical perspective and was what they would be able to take away with them. However the theme of emergence stood out as the principle that generated the most awareness and growth and which, in turn, affected the participants’ autonomy.
- Full Text:
- Authors: Lee, Amanda Jane
- Date: 2014
- Subjects: Mathematics -- Study and teaching -- South Africa , Mathematics teachers -- Training of -- South Africa , Mathematics -- Study and teaching (Elementary) -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2004 , http://hdl.handle.net/10962/d1015664
- Description: This research study investigated the ways in which a mathematics module, informed by an enactivist philosophy, enabled pre-service teachers to unpack the reality of their teaching practice in terms of proficient teaching. Given the generally poor mathematics results in South Africa it is not enough for teachers to be merely proficient in Mathematics. They also need to be in a position to explain important mathematical concepts to children in a manner that will encourage and develop an understanding of the basic mathematical concepts. It was my intention with this study to determine whether a mathematics education module, that embraced the underlying themes of enactivism as part of its teaching pedagogy, could have the potential to develop and increase the skills of pre-service teachers’ teaching for proficiency in Mathematics. The mathematics module was underpinned by five themes of enactivism namely: autonomy, embodiment, emergence, sense-making and experience and was designed to supplement the pre-service teachers’ basic skills in Mathematics in the Intermediate Phase. This mathematics module was offered to fourth year pre-service teachers completing a B.Ed. in the Foundation Phase at a private institute specialising in the training of teachers. The theoretical framework was informed by enactivism and how the themes of enactivism could be used as a vehicle to develop teaching proficiency. The study was qualitative in nature and situated within an interpretivist paradigm. The specific perspectives of interpretivism that were used were hermeneutics, phenomenology and reflexivity. The research design was a case study that contained elements of action research and encompassed three phases of data collection. The first phase focused on the pre-service teachers’ approach to teaching Mathematics and what this brought forth in terms of the reality of their teaching practice and the problems they encountered. The second phase undertook to determine what growth and development of teaching proficiency in Mathematics had emerged over the research period. The final phase was undertaken after the pre-service teachers had graduated and were employed as full time teachers in the Intermediate Phase. The analytical framework and lens through which the data was analysed was that of Kilpatrick, Swafford and Findell’s (2001) strands of mathematical proficiency. The argument that I present is that the themes of enactivism did contribute to the growth of the pre-service teachers’ teaching for mathematical proficiency. The themes of embodiment and experience were major contributions in revealing that this was a reality for the pre-service teachers from a practical perspective and was what they would be able to take away with them. However the theme of emergence stood out as the principle that generated the most awareness and growth and which, in turn, affected the participants’ autonomy.
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A formalised ontology for network attack classification
- Authors: Van Heerden, Renier Pelser
- Date: 2014
- Subjects: Computer networks -- Security measures Computer security Computer crimes -- Investigation Computer crimes -- Prevention
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4691 , http://hdl.handle.net/10962/d1011603
- Description: One of the most popular attack vectors against computers are their network connections. Attacks on computers through their networks are commonplace and have various levels of complexity. This research formally describes network-based computer attacks in the form of a story, formally and within an ontology. The ontology categorises network attacks where attack scenarios are the focal class. This class consists of: Denial-of- Service, Industrial Espionage, Web Defacement, Unauthorised Data Access, Financial Theft, Industrial Sabotage, Cyber-Warfare, Resource Theft, System Compromise, and Runaway Malware. This ontology was developed by building a taxonomy and a temporal network attack model. Network attack instances (also know as individuals) are classified according to their respective attack scenarios, with the use of an automated reasoner within the ontology. The automated reasoner deductions are verified formally; and via the automated reasoner, a relaxed set of scenarios is determined, which is relevant in a near real-time environment. A prototype system (called Aeneas) was developed to classify network-based attacks. Aeneas integrates the sensors into a detection system that can classify network attacks in a near real-time environment. To verify the ontology and the prototype Aeneas, a virtual test bed was developed in which network-based attacks were generated to verify the detection system. Aeneas was able to detect incoming attacks and classify them according to their scenario. The novel part of this research is the attack scenarios that are described in the form of a story, as well as formally and in an ontology. The ontology is used in a novel way to determine to which class attack instances belong and how the network attack ontology is affected in a near real-time environment.
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- Authors: Van Heerden, Renier Pelser
- Date: 2014
- Subjects: Computer networks -- Security measures Computer security Computer crimes -- Investigation Computer crimes -- Prevention
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4691 , http://hdl.handle.net/10962/d1011603
- Description: One of the most popular attack vectors against computers are their network connections. Attacks on computers through their networks are commonplace and have various levels of complexity. This research formally describes network-based computer attacks in the form of a story, formally and within an ontology. The ontology categorises network attacks where attack scenarios are the focal class. This class consists of: Denial-of- Service, Industrial Espionage, Web Defacement, Unauthorised Data Access, Financial Theft, Industrial Sabotage, Cyber-Warfare, Resource Theft, System Compromise, and Runaway Malware. This ontology was developed by building a taxonomy and a temporal network attack model. Network attack instances (also know as individuals) are classified according to their respective attack scenarios, with the use of an automated reasoner within the ontology. The automated reasoner deductions are verified formally; and via the automated reasoner, a relaxed set of scenarios is determined, which is relevant in a near real-time environment. A prototype system (called Aeneas) was developed to classify network-based attacks. Aeneas integrates the sensors into a detection system that can classify network attacks in a near real-time environment. To verify the ontology and the prototype Aeneas, a virtual test bed was developed in which network-based attacks were generated to verify the detection system. Aeneas was able to detect incoming attacks and classify them according to their scenario. The novel part of this research is the attack scenarios that are described in the form of a story, as well as formally and in an ontology. The ontology is used in a novel way to determine to which class attack instances belong and how the network attack ontology is affected in a near real-time environment.
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A lignocellulolytic enzyme system for fruit waste degradation : commercial enzyme mixture synergy and bioreactor design
- Authors: Gama, Repson
- Date: 2014
- Subjects: Enzymes -- Biotechnology , Enzymes -- Industrial applications , Lignocellulose -- Biodegradation , Biomass energy , Biomass conversion , Biochemical engineering , Agricultural wastes as fuel
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4114 , http://hdl.handle.net/10962/d1013073
- Description: Studies into sources of alternative liquid transport fuel energy have identified agro-industrial wastes, which are lignocellulosic in nature, as a potential feedstock for biofuel production against the background of depleting nonrenewable fossil fuels. In South Africa, large quantities of apple and other fruit wastes, called pomace, are generated from fruit and juice industries. Apple pomace is a rich source of cellulose, pectin and hemicellulose, making it a potential target for utilisation as a lignocellulosic feedstock for biofuel and biorefinery chemical production. Lignocellulosic biomass is recalcitrant in nature and therefore its degradation requires the synergistic action of a number of enzymes such as cellulases, hemicellulases, pectinases and ligninases. Commercial enzyme cocktails, containing some of these enzymes, are available and can be used for apple pomace degradation. In this study, the degradation of apple pomace using commercial enzyme cocktails was investigated. The main focus was the optimisation of the release of sugar monomers that could potentially be used for biofuel and biorefinery chemical production. There is no or little information reported in literature on the enzymatic degradation of fruit waste using commercial enzyme mixtures. This study first focused on the characterisation of the substrate (apple pomace) and the commercial enzyme cocktails. Apple pomace was found to contain mainly glucose, galacturonic acid, arabinose, galactose, lignin and low amounts of xylose and fructose. Three commercial enzyme cocktails were initially selected: Biocip Membrane, Viscozyme L (from Aspergillus aculeatus) and Celluclast 1.5L (a Trichoderma reesei ATCC 26921 cellulase preparation). The selection of the enzymes was based on activities declared by the manufacturers, cost and local availability. The enzymes were screened based on their synergistic cooperation in the degradation of apple pomace and the main enzymes present in each cocktail. Viscozyme L and Celluclast 1.5L, in a 50:50 ratio, resulted in the best degree of synergy (1.6) compared to any other combination. The enzyme ratios were determined on Viscozyme L and Celluclast 1.5L based on the protein ratio. Enzyme activity was determined as glucose equivalents using the dinitrosalicylic acid (DNS) method. Sugar monomers were determined using Megazyme assay kits. There is limited information available on the enzymes present in the commercial enzyme cocktails. Therefore, the main enzymes present in Viscozyme L and Celluclast 1.5L were identified using different substrates, each targeted for a specific enzyme and activity. Characterisation of the enzyme mixtures revealed a large number of enzymes required for apple pomace degradation and these included cellulases, pectinases, xylanases, arabinases and mannanases in different proportions. Viscozyme L contained mainly pectinases and hemicellulases, while Celluclast 1.5L displayed largely cellulase and xylanase activity, hence the high degree of synergy reported. The temperature optimum was 50ºC for both enzyme mixtures and pH optima were observed at pH 5.0 and pH 3.0 for Viscozyme L and Celluclast 1.5L, respectively. At 37ºC and pH 5.0, the enzymes retained more that 90% activity after 15 days of incubation, allowing the enzymes to be used together with less energy input. The enzymes were further characterised by determining the effect of various compounds, such as alcohols, sugars, phenolic compounds and metal ions at various concentrations on the activity of the enzymes during apple pomace hydrolysis. Apart from lignin, which had almost no effect on enzyme activity, all the compounds caused inhibition of the enzymes to varying degrees. The most inhibitory compounds were some organic acids and metal ions, as well as cellobiose and xylobiose. Using the best ratio for Viscozyme L and Celluclast 1.5L (50:50) for the hydrolysis of apple pomace, it was observed that synergy was highest at the initial stages of hydrolysis and decreased over time, though the sugar concentration increased. The type of synergy for optimal apple pomace hydrolysis was found to be simultaneous. There was no synergy observed between Viscozyme L and Celluclast 1.5L with ligninases - laccase, lignin peroxidase and manganese peroxidase. Hydrolysing apple pomace with ligninases prior to addition of Viscozyme L and Celluclast 1.5L did not improve degradation of the substrate. Immobilisation of the enzyme mixtures on different supports was performed with the aim of increasing stability and enabling reuse of the enzymes. Immobilisation methods were selected based on the chemical properties of the supports, availability, cost and applicability on heterogeneous and insoluble substrate like apple pomace. These methods included crosslinked enzyme aggregates (CLEAs), immobilisation on various supports such as nylon mesh, nylon beads, sodium alginate beads, chitin and silica gel beads. The immobilisation strategies were unsuccessful, mainly due to the low percentage of immobilisation of the enzyme on the matrix and loss of activity of the immobilised enzyme. Free enzymes were therefore used for the remainder of the study. Hydrolysis conditions for apple pomace degradation were optimised using different temperatures and buffer systems in 1 L volumes mixed with compressed air. Hydrolysis at room temperature, using an unbuffered system, gave a better performance as compared to a buffered system. Reactors operated in batch mode performed better (4.2 g/L (75% yield) glucose and 16.8 g/L (75%) reducing sugar) than fed-batch reactors (3.2 g/L (66%) glucose and 14.6 g/L (72.7% yield) reducing sugar) over 100 h using Viscozyme L and Celluclast 1.5L. Supplementation of β- glucosidase activity in Viscozyme L and Celluclast 1.5L with Novozyme 188 resulted in a doubling of the amount of glucose released. The main products released from apple pomace hydrolysis were galacturonic acid, glucose and arabinose and low amounts of galactose and xylose. These products are potential raw materials for biofuel and biorefinery chemical production. An artificial neural network (ANN) model was successfully developed and used for predicting the optimum conditions for apple pomace hydrolysis using Celluclast 1.5L, Viscozyme L and Novozyme 188. Four main conditions that affect apple pomace hydrolysis were selected, namely temperature, initial pH, enzyme loading and substrate loading, which were taken as inputs. The glucose and reducing sugars released as a result of each treatment and their combinations were taken as outputs for 1–100 h. An ANN with 20, 20 and 6 neurons in the first, second and third hidden layers, respectively, was constructed. The performance and predictive ability of the ANN was good, with a R² of 0.99 and a small mean square error (MSE). New data was successfully predicted and simulated. Optimal hydrolysis conditions predicted by ANN for apple pomace hydrolysis were at 30% substrate (wet w/v) and an enzyme loading of 0.5 mg/g and 0.2 mg/mL of substrate for glucose and reducing sugar, respectively, giving sugar concentrations of 6.5 mg/mL and 28.9 mg/mL for glucose and reducing sugar, respectively. ANN showed that enzyme and substrate loadings were the most important factors for the hydrolysis of apple pomace.
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- Authors: Gama, Repson
- Date: 2014
- Subjects: Enzymes -- Biotechnology , Enzymes -- Industrial applications , Lignocellulose -- Biodegradation , Biomass energy , Biomass conversion , Biochemical engineering , Agricultural wastes as fuel
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4114 , http://hdl.handle.net/10962/d1013073
- Description: Studies into sources of alternative liquid transport fuel energy have identified agro-industrial wastes, which are lignocellulosic in nature, as a potential feedstock for biofuel production against the background of depleting nonrenewable fossil fuels. In South Africa, large quantities of apple and other fruit wastes, called pomace, are generated from fruit and juice industries. Apple pomace is a rich source of cellulose, pectin and hemicellulose, making it a potential target for utilisation as a lignocellulosic feedstock for biofuel and biorefinery chemical production. Lignocellulosic biomass is recalcitrant in nature and therefore its degradation requires the synergistic action of a number of enzymes such as cellulases, hemicellulases, pectinases and ligninases. Commercial enzyme cocktails, containing some of these enzymes, are available and can be used for apple pomace degradation. In this study, the degradation of apple pomace using commercial enzyme cocktails was investigated. The main focus was the optimisation of the release of sugar monomers that could potentially be used for biofuel and biorefinery chemical production. There is no or little information reported in literature on the enzymatic degradation of fruit waste using commercial enzyme mixtures. This study first focused on the characterisation of the substrate (apple pomace) and the commercial enzyme cocktails. Apple pomace was found to contain mainly glucose, galacturonic acid, arabinose, galactose, lignin and low amounts of xylose and fructose. Three commercial enzyme cocktails were initially selected: Biocip Membrane, Viscozyme L (from Aspergillus aculeatus) and Celluclast 1.5L (a Trichoderma reesei ATCC 26921 cellulase preparation). The selection of the enzymes was based on activities declared by the manufacturers, cost and local availability. The enzymes were screened based on their synergistic cooperation in the degradation of apple pomace and the main enzymes present in each cocktail. Viscozyme L and Celluclast 1.5L, in a 50:50 ratio, resulted in the best degree of synergy (1.6) compared to any other combination. The enzyme ratios were determined on Viscozyme L and Celluclast 1.5L based on the protein ratio. Enzyme activity was determined as glucose equivalents using the dinitrosalicylic acid (DNS) method. Sugar monomers were determined using Megazyme assay kits. There is limited information available on the enzymes present in the commercial enzyme cocktails. Therefore, the main enzymes present in Viscozyme L and Celluclast 1.5L were identified using different substrates, each targeted for a specific enzyme and activity. Characterisation of the enzyme mixtures revealed a large number of enzymes required for apple pomace degradation and these included cellulases, pectinases, xylanases, arabinases and mannanases in different proportions. Viscozyme L contained mainly pectinases and hemicellulases, while Celluclast 1.5L displayed largely cellulase and xylanase activity, hence the high degree of synergy reported. The temperature optimum was 50ºC for both enzyme mixtures and pH optima were observed at pH 5.0 and pH 3.0 for Viscozyme L and Celluclast 1.5L, respectively. At 37ºC and pH 5.0, the enzymes retained more that 90% activity after 15 days of incubation, allowing the enzymes to be used together with less energy input. The enzymes were further characterised by determining the effect of various compounds, such as alcohols, sugars, phenolic compounds and metal ions at various concentrations on the activity of the enzymes during apple pomace hydrolysis. Apart from lignin, which had almost no effect on enzyme activity, all the compounds caused inhibition of the enzymes to varying degrees. The most inhibitory compounds were some organic acids and metal ions, as well as cellobiose and xylobiose. Using the best ratio for Viscozyme L and Celluclast 1.5L (50:50) for the hydrolysis of apple pomace, it was observed that synergy was highest at the initial stages of hydrolysis and decreased over time, though the sugar concentration increased. The type of synergy for optimal apple pomace hydrolysis was found to be simultaneous. There was no synergy observed between Viscozyme L and Celluclast 1.5L with ligninases - laccase, lignin peroxidase and manganese peroxidase. Hydrolysing apple pomace with ligninases prior to addition of Viscozyme L and Celluclast 1.5L did not improve degradation of the substrate. Immobilisation of the enzyme mixtures on different supports was performed with the aim of increasing stability and enabling reuse of the enzymes. Immobilisation methods were selected based on the chemical properties of the supports, availability, cost and applicability on heterogeneous and insoluble substrate like apple pomace. These methods included crosslinked enzyme aggregates (CLEAs), immobilisation on various supports such as nylon mesh, nylon beads, sodium alginate beads, chitin and silica gel beads. The immobilisation strategies were unsuccessful, mainly due to the low percentage of immobilisation of the enzyme on the matrix and loss of activity of the immobilised enzyme. Free enzymes were therefore used for the remainder of the study. Hydrolysis conditions for apple pomace degradation were optimised using different temperatures and buffer systems in 1 L volumes mixed with compressed air. Hydrolysis at room temperature, using an unbuffered system, gave a better performance as compared to a buffered system. Reactors operated in batch mode performed better (4.2 g/L (75% yield) glucose and 16.8 g/L (75%) reducing sugar) than fed-batch reactors (3.2 g/L (66%) glucose and 14.6 g/L (72.7% yield) reducing sugar) over 100 h using Viscozyme L and Celluclast 1.5L. Supplementation of β- glucosidase activity in Viscozyme L and Celluclast 1.5L with Novozyme 188 resulted in a doubling of the amount of glucose released. The main products released from apple pomace hydrolysis were galacturonic acid, glucose and arabinose and low amounts of galactose and xylose. These products are potential raw materials for biofuel and biorefinery chemical production. An artificial neural network (ANN) model was successfully developed and used for predicting the optimum conditions for apple pomace hydrolysis using Celluclast 1.5L, Viscozyme L and Novozyme 188. Four main conditions that affect apple pomace hydrolysis were selected, namely temperature, initial pH, enzyme loading and substrate loading, which were taken as inputs. The glucose and reducing sugars released as a result of each treatment and their combinations were taken as outputs for 1–100 h. An ANN with 20, 20 and 6 neurons in the first, second and third hidden layers, respectively, was constructed. The performance and predictive ability of the ANN was good, with a R² of 0.99 and a small mean square error (MSE). New data was successfully predicted and simulated. Optimal hydrolysis conditions predicted by ANN for apple pomace hydrolysis were at 30% substrate (wet w/v) and an enzyme loading of 0.5 mg/g and 0.2 mg/mL of substrate for glucose and reducing sugar, respectively, giving sugar concentrations of 6.5 mg/mL and 28.9 mg/mL for glucose and reducing sugar, respectively. ANN showed that enzyme and substrate loadings were the most important factors for the hydrolysis of apple pomace.
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