Assessing the potential impact of climate change in the south-eastern Great Escarpment, Southern Africa
- Authors: Bentley, Luke Kinross
- Date: 2017
- Subjects: Mountain plants -- Climatic factors -- Drakensberg Mountains , Mountain biodiversity -- Drakensberg Mountains , Climatic changes -- Drakensberg Mountains , Rain and rainfall -- Drakensberg Mountains , Plant diversity -- Climatic factors -- Drakensberg Mountains
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4657 , vital:20709
- Description: Rapid, anthropogenic climate change is a contemporary phenomenon which is threatening natural ecosystems. Mountains are globally important, housing high levels of plant and animal biodiversity, as well as being important suppliers of water with 50% of mountainous regions being essential for the provision of water to downstream inhabitants. Owing to their importance, an assessment of the historical, current and potential impacts of climate change on the south-eastern Great Escarpment encompassing South Africa and Lesotho is necessary and is the primary aim of this thesis. In order to assess the historical and current impacts of climate change, historical rainfall records were collected from 78 sites throughout the Eastern Cape. These records were used to determine rainfall trends and trends in the frequency of daily extreme events. Climate change projections predict increasing trends in summer and annual rainfall, and a marginal increase to a slightly decreasing trend in winter rainfall, depending on if statistically downscaled or dynamically downscaled projections are examined. Extreme events are also predicted to increase in frequency. Results showed trends which generally agree with the predictions, except for extreme events for which there was insufficient data to support the projections. This has implications for farmers in the area and validates the downscaled Regional Climate Models (RCMs) to be used for the predictive analysis determining the potential future impacts of climate change on plant species' distributions in the south-eastern Great Escarpment. In order to assess montane plant species to future climate predictions, occurrence records of 46 species were correlated to rainfall and temperature predictor variables and predictions for species richness and beta diversity were made for current and future climates. This predictive analysis used and compared two correlative species distribution modelling methods - one method used one correlating algorithm in a nuanced manner and the other method used five correlating algorithms in an ensemble. A trend of range restriction to higher elevations for the 46 species analysed was as expected from a warming climate. The nuanced, single algorithm modelling method produced less conservative models. The predicted trend of an elevational increase in montane vegetation in this region points to Lesotho and the Drakensberg highlands as being an important refugium for montane plant taxa of the southeastern Great Escarpment. The Maloti Drakensberg Transfrontier Conservation Area hence needs to be expanded and its mandate properly enforced to protect this region adequately.
- Full Text:
- Date Issued: 2017
- Authors: Bentley, Luke Kinross
- Date: 2017
- Subjects: Mountain plants -- Climatic factors -- Drakensberg Mountains , Mountain biodiversity -- Drakensberg Mountains , Climatic changes -- Drakensberg Mountains , Rain and rainfall -- Drakensberg Mountains , Plant diversity -- Climatic factors -- Drakensberg Mountains
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4657 , vital:20709
- Description: Rapid, anthropogenic climate change is a contemporary phenomenon which is threatening natural ecosystems. Mountains are globally important, housing high levels of plant and animal biodiversity, as well as being important suppliers of water with 50% of mountainous regions being essential for the provision of water to downstream inhabitants. Owing to their importance, an assessment of the historical, current and potential impacts of climate change on the south-eastern Great Escarpment encompassing South Africa and Lesotho is necessary and is the primary aim of this thesis. In order to assess the historical and current impacts of climate change, historical rainfall records were collected from 78 sites throughout the Eastern Cape. These records were used to determine rainfall trends and trends in the frequency of daily extreme events. Climate change projections predict increasing trends in summer and annual rainfall, and a marginal increase to a slightly decreasing trend in winter rainfall, depending on if statistically downscaled or dynamically downscaled projections are examined. Extreme events are also predicted to increase in frequency. Results showed trends which generally agree with the predictions, except for extreme events for which there was insufficient data to support the projections. This has implications for farmers in the area and validates the downscaled Regional Climate Models (RCMs) to be used for the predictive analysis determining the potential future impacts of climate change on plant species' distributions in the south-eastern Great Escarpment. In order to assess montane plant species to future climate predictions, occurrence records of 46 species were correlated to rainfall and temperature predictor variables and predictions for species richness and beta diversity were made for current and future climates. This predictive analysis used and compared two correlative species distribution modelling methods - one method used one correlating algorithm in a nuanced manner and the other method used five correlating algorithms in an ensemble. A trend of range restriction to higher elevations for the 46 species analysed was as expected from a warming climate. The nuanced, single algorithm modelling method produced less conservative models. The predicted trend of an elevational increase in montane vegetation in this region points to Lesotho and the Drakensberg highlands as being an important refugium for montane plant taxa of the southeastern Great Escarpment. The Maloti Drakensberg Transfrontier Conservation Area hence needs to be expanded and its mandate properly enforced to protect this region adequately.
- Full Text:
- Date Issued: 2017
An investigation of the aquatic macroinvertebrate fauna of the southern Great Escarpment (South Africa): insights from ecological and genetic studies
- Authors: Taylor, Chantal Lee
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4275 , http://hdl.handle.net/10962/d1020839
- Description: Biological diversity in freshwater biomes is vital to maintain healthy, functioning ecosystems with resilience to disturbance and the impacts of climate change. Freshwater ecosystems provide essential resources to life on Earth. However, as increasing pressure is being placed on the environment by human population growth, the quality of freshwater resources and the health of these ecosystems are at risk. Mountain streams provide an important source of water and are usually less affected by anthropogenic stressors, compared to lowland freshwaters. These montane streams are therefore of important conservation value and due to their untransformed nature serve as ideal ecosystems for biodiversity studies and as reference sites for studies on environmental change. This study explores aquatic macroinvertebrate biodiversity of the southern Great Escarpment in South Africa. Species assemblages and the environmental variables of each site were sampled from first order streams across five different mountain blocks along the Great Escarpment. Additionally, mitochondrial DNA of three mayfly species (Afroptilum sudafricanum, Demoreptus natalensis and Demoreptus capensis), commonly occurring in the study area, was analysed to compare the genetic diversity between habitat specialist and habitat generalist species. A total of 2 595 macroinvertebrate specimens from 47 families and 86 species were collected with several interesting and potentially new species being discovered. Partitioned diversity analyses indicate that macroinvertebrate diversity varies across mountain blocks. Multivariate analyses indicate that differences in assemblages could be attributed to differences in environmental variables between sites, particularly water velocity, total dissolved solids and salinity. As these environmental variables reflect of the topography of the sites, differences in species assemblages was attributed to difference in topography and therefore biotopes present. Habitat-restricted mayfly species (D. natalensis and D. capensis), occur in distinct populations confined to mountains blocks. Isolation-by-distance analyses further emphasis that these species are genetically restricted by their habitat preference for mountain streams. In contrast, A. sudafricanum, a habitat generalist, showed no indication of genetic structure due to location or distance. Possible cryptic taxa and new species were identified within A. sudafricanum and Dermoreptus respectively. This study provides an important contribution to the baseline data of freshwater macroinvertebrate diversity for the southern Great Escarpment region and provides insights into the considerable genetic diversity of selected aquatic taxa across this region.
- Full Text:
- Date Issued: 2016
- Authors: Taylor, Chantal Lee
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4275 , http://hdl.handle.net/10962/d1020839
- Description: Biological diversity in freshwater biomes is vital to maintain healthy, functioning ecosystems with resilience to disturbance and the impacts of climate change. Freshwater ecosystems provide essential resources to life on Earth. However, as increasing pressure is being placed on the environment by human population growth, the quality of freshwater resources and the health of these ecosystems are at risk. Mountain streams provide an important source of water and are usually less affected by anthropogenic stressors, compared to lowland freshwaters. These montane streams are therefore of important conservation value and due to their untransformed nature serve as ideal ecosystems for biodiversity studies and as reference sites for studies on environmental change. This study explores aquatic macroinvertebrate biodiversity of the southern Great Escarpment in South Africa. Species assemblages and the environmental variables of each site were sampled from first order streams across five different mountain blocks along the Great Escarpment. Additionally, mitochondrial DNA of three mayfly species (Afroptilum sudafricanum, Demoreptus natalensis and Demoreptus capensis), commonly occurring in the study area, was analysed to compare the genetic diversity between habitat specialist and habitat generalist species. A total of 2 595 macroinvertebrate specimens from 47 families and 86 species were collected with several interesting and potentially new species being discovered. Partitioned diversity analyses indicate that macroinvertebrate diversity varies across mountain blocks. Multivariate analyses indicate that differences in assemblages could be attributed to differences in environmental variables between sites, particularly water velocity, total dissolved solids and salinity. As these environmental variables reflect of the topography of the sites, differences in species assemblages was attributed to difference in topography and therefore biotopes present. Habitat-restricted mayfly species (D. natalensis and D. capensis), occur in distinct populations confined to mountains blocks. Isolation-by-distance analyses further emphasis that these species are genetically restricted by their habitat preference for mountain streams. In contrast, A. sudafricanum, a habitat generalist, showed no indication of genetic structure due to location or distance. Possible cryptic taxa and new species were identified within A. sudafricanum and Dermoreptus respectively. This study provides an important contribution to the baseline data of freshwater macroinvertebrate diversity for the southern Great Escarpment region and provides insights into the considerable genetic diversity of selected aquatic taxa across this region.
- Full Text:
- Date Issued: 2016
The phytogeography of Southern Mistbelt Forests of the Eastern Cape, South Africa
- Authors: Matiwane, Aviwe
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3224 , vital:20393
- Description: In the Eastern Cape, a moisture gradient (from west to east) exists along the Escarpment where patches of Southern Mistbelt Forests (SMF) occur. There is a notable profusion of plants in these forests, but the factors that drive plant species composition and abundance in these patches is poorly understood and such knowledge is critical if we are to address or mitigate the problems imposed by global change. Many of our ideas on plant community assembly are based on the premise that species differ in their environmental requirements for successful regeneration, especially when young. The aims of this study were to document the floristic diversity of poorly known patches of Southern Mistbelt Forests in the Eastern Cape and identify the environmental factors that influence plant community composition in these forest patches. Twenty six forest patches across the Eastern Cape were sampled. In each forest 400m2 circular plots were constructed. The diameter at breast height and stem counts for the tree species was taken and the % cover of the herbaceous species was recorded. General collections were also made to supplement the plot data in order to obtain a comprehensive flora for each forest patch. All sampling was done during the summer season of 2013 and 2014. Using plot data, Hierarchal Cluster Analysis and Multidimensional Scaling Analysis were done to compare similarities between patches. Plots from the same forest patch clustered which indicated that the species composition in each forest patch was unique. However, this pattern went down when only tree species were considered. Plant diversity also varied between patches; with those located in the east being generally more diverse than those in the west are. Clustering patterns in fragmented communities and differences in species diversity indicate evidence of species “nestedness”. Analysis of nestedness indicated that these forest patches were significantly nested. To identify which environmental variables might be correlated with both species diversity and nestedness, twelve environmental variables was selected for analysis using Canonical Correspondence Analysis and Principal Component Analysis. Results show that Mean Annual Precipitation, Mean Annual Potential Evapotranspiration, and percentage of Organic Matter of the soil were most strongly correlated with the patterns of diversity observed in these forest patches. Regression analysis indicated that Mean Annual Precipitation accounted for most of the variation in species diversity, especially for the herbaceous species. The herbaceous species thus show a greater sensitivity to “climate filtering” as a consequence of rainfall gradients. With the implementation of suitable monitoring programs, changes in abundance or even local extinction of these species can act as an early warning indicator of the possible long-term effects of climate change on forest communities.
- Full Text:
- Date Issued: 2016
- Authors: Matiwane, Aviwe
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3224 , vital:20393
- Description: In the Eastern Cape, a moisture gradient (from west to east) exists along the Escarpment where patches of Southern Mistbelt Forests (SMF) occur. There is a notable profusion of plants in these forests, but the factors that drive plant species composition and abundance in these patches is poorly understood and such knowledge is critical if we are to address or mitigate the problems imposed by global change. Many of our ideas on plant community assembly are based on the premise that species differ in their environmental requirements for successful regeneration, especially when young. The aims of this study were to document the floristic diversity of poorly known patches of Southern Mistbelt Forests in the Eastern Cape and identify the environmental factors that influence plant community composition in these forest patches. Twenty six forest patches across the Eastern Cape were sampled. In each forest 400m2 circular plots were constructed. The diameter at breast height and stem counts for the tree species was taken and the % cover of the herbaceous species was recorded. General collections were also made to supplement the plot data in order to obtain a comprehensive flora for each forest patch. All sampling was done during the summer season of 2013 and 2014. Using plot data, Hierarchal Cluster Analysis and Multidimensional Scaling Analysis were done to compare similarities between patches. Plots from the same forest patch clustered which indicated that the species composition in each forest patch was unique. However, this pattern went down when only tree species were considered. Plant diversity also varied between patches; with those located in the east being generally more diverse than those in the west are. Clustering patterns in fragmented communities and differences in species diversity indicate evidence of species “nestedness”. Analysis of nestedness indicated that these forest patches were significantly nested. To identify which environmental variables might be correlated with both species diversity and nestedness, twelve environmental variables was selected for analysis using Canonical Correspondence Analysis and Principal Component Analysis. Results show that Mean Annual Precipitation, Mean Annual Potential Evapotranspiration, and percentage of Organic Matter of the soil were most strongly correlated with the patterns of diversity observed in these forest patches. Regression analysis indicated that Mean Annual Precipitation accounted for most of the variation in species diversity, especially for the herbaceous species. The herbaceous species thus show a greater sensitivity to “climate filtering” as a consequence of rainfall gradients. With the implementation of suitable monitoring programs, changes in abundance or even local extinction of these species can act as an early warning indicator of the possible long-term effects of climate change on forest communities.
- Full Text:
- Date Issued: 2016
Montane Wetlands of the South African Great Escarpment : plant communities and environmental drivers
- Authors: Janks, Matthew Richard
- Date: 2015
- Subjects: Mountain plants -- South Africa -- Eastern Cape , Wetland plants -- South Africa -- Eastern Cape , Wetland plants -- Effect of altitude on -- South Africa -- Eastern Cape , Wetland ecology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4274 , http://hdl.handle.net/10962/d1018917
- Description: Wetlands provide a number of valuable functions to both the surrounding environment and society. The anaerobic conditions created by flooding in wetlands provide a habitat that supports unique assemblages of plant life. High altitude wetlands are amongst the most species-rich in South Africa. They house a number of rare species and play a vital role in the supply of water to lower lying areas. These are some of the reasons that mountain wetlands are of high conservation value. A phytosociological study was undertaken on the high altitude wetlands of the Great Escarpment with the aim of classifying the plant communities and identifying the environmental drivers of plant community patterns within these ecosystems. Data collection was focused in the Eastern Cape and was supplemented with data from existing studies to gain a more complete understanding of the wetlands of the Great Escarpment of South Africa. Using the Braun-Blanquet approach, Hierarchical Cluster Analysis and Indicator Species Analysis; five broad wetland groups were identified, comprised of 33 individual plant communities and 81 indicator species. Multivariate analysis, including Canonical Correspondence Analysis revealed that the effects of altitude, such as temperature and rainfall, are the most significant large-scale drivers of vegetation patterns. Smaller scale drivers include wetness and soil nutrients including nitrogen, phosphorus, electrical conductivity, sodium, and organic content. The identification of indicator species served to reveal potentially important wetland species across different areas of the Great Escarpment. The effects of altitude on plant community patterns highlights the susceptibility of the high altitude specific communities to upward temperature zone shifts resulting from global warming. Other threats include livestock trampling, water extraction, and land use change for agricultural purposes. The relative absence of alien species in these wetlands gives an indication of their pristine condition and therefore their importance as a reference from which they may be monitored. A large proportion of the wetlands studied here occur outside protected areas, and given the rate of wetland loss in South Africa, it is important that continued effective land management is practiced to ensure that these ecosystems are conserved in the future .
- Full Text:
- Date Issued: 2015
Montane Wetlands of the South African Great Escarpment : plant communities and environmental drivers
- Authors: Janks, Matthew Richard
- Date: 2015
- Subjects: Mountain plants -- South Africa -- Eastern Cape , Wetland plants -- South Africa -- Eastern Cape , Wetland plants -- Effect of altitude on -- South Africa -- Eastern Cape , Wetland ecology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4274 , http://hdl.handle.net/10962/d1018917
- Description: Wetlands provide a number of valuable functions to both the surrounding environment and society. The anaerobic conditions created by flooding in wetlands provide a habitat that supports unique assemblages of plant life. High altitude wetlands are amongst the most species-rich in South Africa. They house a number of rare species and play a vital role in the supply of water to lower lying areas. These are some of the reasons that mountain wetlands are of high conservation value. A phytosociological study was undertaken on the high altitude wetlands of the Great Escarpment with the aim of classifying the plant communities and identifying the environmental drivers of plant community patterns within these ecosystems. Data collection was focused in the Eastern Cape and was supplemented with data from existing studies to gain a more complete understanding of the wetlands of the Great Escarpment of South Africa. Using the Braun-Blanquet approach, Hierarchical Cluster Analysis and Indicator Species Analysis; five broad wetland groups were identified, comprised of 33 individual plant communities and 81 indicator species. Multivariate analysis, including Canonical Correspondence Analysis revealed that the effects of altitude, such as temperature and rainfall, are the most significant large-scale drivers of vegetation patterns. Smaller scale drivers include wetness and soil nutrients including nitrogen, phosphorus, electrical conductivity, sodium, and organic content. The identification of indicator species served to reveal potentially important wetland species across different areas of the Great Escarpment. The effects of altitude on plant community patterns highlights the susceptibility of the high altitude specific communities to upward temperature zone shifts resulting from global warming. Other threats include livestock trampling, water extraction, and land use change for agricultural purposes. The relative absence of alien species in these wetlands gives an indication of their pristine condition and therefore their importance as a reference from which they may be monitored. A large proportion of the wetlands studied here occur outside protected areas, and given the rate of wetland loss in South Africa, it is important that continued effective land management is practiced to ensure that these ecosystems are conserved in the future .
- Full Text:
- Date Issued: 2015
The role of seasonality, environmental correlates and edge effects on the diversity and abundance of small mammals in Afromontane forest patches, Eastern Cape, South Africa
- Authors: Junkuhn, Kyle Peter
- Date: 2015
- Subjects: Upland ecology -- South Africa -- Eastern Cape , Mammal populations -- South Africa -- Eastern Cape , Forest animals -- Adaptation -- South Africa -- Eastern Cape , Forest animals -- Climatic factors -- South Africa -- Eastern Cape , Forest animals -- Effect of habitat modification on -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4270 , http://hdl.handle.net/10962/d1018188
- Description: The Eastern Cape contains the majority of the remaining forested areas in South Africa (95.8 percent Coastal forests and 47.6 percent Afromontane forests), however these occur in fragments. Due to the rapid rise in the human population and its needs, conversion of forests to agricultural land and the extraction of timber increases, natural vegetation are lost and this therefore leads to forests becoming fragmented into small forest patches. One of the main consequences of forest fragmentation is loss of contiguous habitat, which is the dominant threat to species globally as it negatively affects both species richness and genetic diversity. This research investigates the effects that forest fragmentation has on small mammal diversity and abundance. The study sampled small mammals in Southern Mistbelt Afromontane forest patches in the Eastern Cape. The first aim of this study was to identify which environmental variable, or combinations of variables, affect the diversity and abundance of small mammals in fragmented forest patches in the Eastern Cape during the austral summer. The second aim was to compare seasonal changes in small mammal diversity and abundance at a forest edge compared to the forest core at Beggars Bush, an Afromontane forest in the Eastern Cape, South Africa. To identify the environmental impacts that different forests have on small mammals, nine different forests were chosen in the Eastern Cape and sampled during the summer in 2013. These nine forests were: Fort Fordyce Forest, Thaba Ndoda Forest, Hogsback Forest, Dassie Kraans Forest, Langeni Forest, Burchell’s Reserve Forest, Maiden Dam Forest and Kagaberg Forest. At each site, three transects of 30 Sherman Traps each were used. Trapping periods within each forest patch lasted between three to five consecutive nights and traps were inspected once a day in the morning. Nine environmental variables were initially identified and after being tested for normality and colinearity, five variables were chosen. These variables were Altitude, Mean Annual Temperature, Gradient, Patch Size and Mean annual potential evaporation. The number of individuals captures and species richness were then incorporated as dependent variables for best subset multiple regression model selection using the Akaike Information Criterion (AIC). For the second aim, the same trapping methods were used when comparing seasonal changes to diversity and abundance within the forest core. However at the forest edge, due to the small size of the forest, only 20 Sherman traps were used in each transect with five traps (25 percent) placed in the grassland and 15 (75 percent) placed in the forest along each transect. This study was one of few to survey small mammal diversity and abundance in fragmented forests in the Eastern Cape. Gradient, patch size and mean annual potential evaporation were the variables that best predicted the individual number of small mammal captures while only gradient best explained species richness. It does however, need to be noted that capture rates and species richness were very low and this would therefore effect the analysis of environmental variables. Future studies should have a larger sample size of forest patches and include more microhabitat environmental variables to determine their effects on small mammal diversity and abundance. However, it should be noted that through climatic extinction filtering, forest mammals are resilient generalists that can tolerate fragmentation effects. Furthermore, it was found that forest edges appear to play a significant role in small mammal diversity and abundance in the Beggars Bush Afromontane forest. Some species were habitat specialists such as Rhabdomys pumilio preferring the grassland habitat and Graphiurus murinus and Aethomys namaquensis the forest habitat, while Myosorex varius was the only species that was not habitat dependant. It was found that there was a greater diversity and abundance at the forest edge compared to within the forest core throughout most of the seasons. One possible flaw was that the sampling methods were different at the edge and within the forest and therefore future studies should ensure that the method stays uniform throughout the study.
- Full Text:
- Date Issued: 2015
- Authors: Junkuhn, Kyle Peter
- Date: 2015
- Subjects: Upland ecology -- South Africa -- Eastern Cape , Mammal populations -- South Africa -- Eastern Cape , Forest animals -- Adaptation -- South Africa -- Eastern Cape , Forest animals -- Climatic factors -- South Africa -- Eastern Cape , Forest animals -- Effect of habitat modification on -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4270 , http://hdl.handle.net/10962/d1018188
- Description: The Eastern Cape contains the majority of the remaining forested areas in South Africa (95.8 percent Coastal forests and 47.6 percent Afromontane forests), however these occur in fragments. Due to the rapid rise in the human population and its needs, conversion of forests to agricultural land and the extraction of timber increases, natural vegetation are lost and this therefore leads to forests becoming fragmented into small forest patches. One of the main consequences of forest fragmentation is loss of contiguous habitat, which is the dominant threat to species globally as it negatively affects both species richness and genetic diversity. This research investigates the effects that forest fragmentation has on small mammal diversity and abundance. The study sampled small mammals in Southern Mistbelt Afromontane forest patches in the Eastern Cape. The first aim of this study was to identify which environmental variable, or combinations of variables, affect the diversity and abundance of small mammals in fragmented forest patches in the Eastern Cape during the austral summer. The second aim was to compare seasonal changes in small mammal diversity and abundance at a forest edge compared to the forest core at Beggars Bush, an Afromontane forest in the Eastern Cape, South Africa. To identify the environmental impacts that different forests have on small mammals, nine different forests were chosen in the Eastern Cape and sampled during the summer in 2013. These nine forests were: Fort Fordyce Forest, Thaba Ndoda Forest, Hogsback Forest, Dassie Kraans Forest, Langeni Forest, Burchell’s Reserve Forest, Maiden Dam Forest and Kagaberg Forest. At each site, three transects of 30 Sherman Traps each were used. Trapping periods within each forest patch lasted between three to five consecutive nights and traps were inspected once a day in the morning. Nine environmental variables were initially identified and after being tested for normality and colinearity, five variables were chosen. These variables were Altitude, Mean Annual Temperature, Gradient, Patch Size and Mean annual potential evaporation. The number of individuals captures and species richness were then incorporated as dependent variables for best subset multiple regression model selection using the Akaike Information Criterion (AIC). For the second aim, the same trapping methods were used when comparing seasonal changes to diversity and abundance within the forest core. However at the forest edge, due to the small size of the forest, only 20 Sherman traps were used in each transect with five traps (25 percent) placed in the grassland and 15 (75 percent) placed in the forest along each transect. This study was one of few to survey small mammal diversity and abundance in fragmented forests in the Eastern Cape. Gradient, patch size and mean annual potential evaporation were the variables that best predicted the individual number of small mammal captures while only gradient best explained species richness. It does however, need to be noted that capture rates and species richness were very low and this would therefore effect the analysis of environmental variables. Future studies should have a larger sample size of forest patches and include more microhabitat environmental variables to determine their effects on small mammal diversity and abundance. However, it should be noted that through climatic extinction filtering, forest mammals are resilient generalists that can tolerate fragmentation effects. Furthermore, it was found that forest edges appear to play a significant role in small mammal diversity and abundance in the Beggars Bush Afromontane forest. Some species were habitat specialists such as Rhabdomys pumilio preferring the grassland habitat and Graphiurus murinus and Aethomys namaquensis the forest habitat, while Myosorex varius was the only species that was not habitat dependant. It was found that there was a greater diversity and abundance at the forest edge compared to within the forest core throughout most of the seasons. One possible flaw was that the sampling methods were different at the edge and within the forest and therefore future studies should ensure that the method stays uniform throughout the study.
- Full Text:
- Date Issued: 2015
Comparative phylogeography of five swallowtail butterfly species (Lepidoptera: Papilionidae) in South Africa : ecological and taxonomic implications.
- Authors: Neef, Götz-Georg
- Date: 2014
- Subjects: Swallowtail butterflies , Papilionidae -- South Africa , Phylogeography -- South Africa , Swallowtail butterflies -- Effect of habitat modification on , Biodiversity -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4266 , http://hdl.handle.net/10962/d1013574
- Description: With current biota under constant threat of extinction, it is important to ascertain where and how biological diversity is generated and partitioned. Phylogeographic studies can assist in the identification of places and processes that indicate the origin and maintenance of biodiversity. Forest fragmentation has a big effect on local extinction and loss of genetic diversity of forest-restricted taxa, along with divergence and speciation of forest biota. This study aims to understand the effects of these processes on a number of forest-dwelling butterflies using a comparative phylogeographic approach. Mitochondrial DNA of five different Papilio species with different degrees of forest specificity was analysed using phylogenetic methods. In addition, the subspecific taxonomy of P. ophidicephalus was investigated using morphometrics of discal spots on the wings and nuclear DNA analysis along with mitochondrial DNA analysis. The results show that the forest-restricted species (P. ophidicephalus and P. echerioides) have more genetic structure and less genetic diversity than the more generalist species (P. dardanus, P. demodocus and P. nireus). This could be due to inbreeding depression and bottlenecks caused by forest fragmentation. As forest patches become smaller, the population size is affected and that causes a loss in genetic diversity, and increasing habitat fragmentation disrupts gene flow. The intraspecific taxonomy of P. ophidicephalus is far from revealed. However, this study shows there is evidence for the different subspecies when comparing morphological results and genetic results. From the evidence provided here it is suggested that P. ophidicephalus should be divided into two separate species rather than five subspecies.
- Full Text:
- Date Issued: 2014
- Authors: Neef, Götz-Georg
- Date: 2014
- Subjects: Swallowtail butterflies , Papilionidae -- South Africa , Phylogeography -- South Africa , Swallowtail butterflies -- Effect of habitat modification on , Biodiversity -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4266 , http://hdl.handle.net/10962/d1013574
- Description: With current biota under constant threat of extinction, it is important to ascertain where and how biological diversity is generated and partitioned. Phylogeographic studies can assist in the identification of places and processes that indicate the origin and maintenance of biodiversity. Forest fragmentation has a big effect on local extinction and loss of genetic diversity of forest-restricted taxa, along with divergence and speciation of forest biota. This study aims to understand the effects of these processes on a number of forest-dwelling butterflies using a comparative phylogeographic approach. Mitochondrial DNA of five different Papilio species with different degrees of forest specificity was analysed using phylogenetic methods. In addition, the subspecific taxonomy of P. ophidicephalus was investigated using morphometrics of discal spots on the wings and nuclear DNA analysis along with mitochondrial DNA analysis. The results show that the forest-restricted species (P. ophidicephalus and P. echerioides) have more genetic structure and less genetic diversity than the more generalist species (P. dardanus, P. demodocus and P. nireus). This could be due to inbreeding depression and bottlenecks caused by forest fragmentation. As forest patches become smaller, the population size is affected and that causes a loss in genetic diversity, and increasing habitat fragmentation disrupts gene flow. The intraspecific taxonomy of P. ophidicephalus is far from revealed. However, this study shows there is evidence for the different subspecies when comparing morphological results and genetic results. From the evidence provided here it is suggested that P. ophidicephalus should be divided into two separate species rather than five subspecies.
- Full Text:
- Date Issued: 2014
Pollinator mediated selection in Pelargonium reniforme Curtis (Geraniaceae) : patterns and process
- Authors: De Wet, Leigh-Ann Robynne
- Date: 2013-06-25
- Subjects: Pelargoniums -- Classification , Pelargoniums -- Pollen , Pelargoniums -- Selection
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4255 , http://hdl.handle.net/10962/d1008185 , Pelargoniums -- Classification , Pelargoniums -- Pollen , Pelargoniums -- Selection
- Description: Pelargonium reniforme is currently divided into two subspecies, P. reniforme subsp. reniforme and P. reniforme subsp. velutinum. The species falls into section Reniformia along with the closely-related P. sidoides. Observations of the section showed some discrepancies in the current taxonomy; mainly floral variation that was not recorded in the descriptions of the subspecies of P. reniforme, particularly the differences in hypanthium lengths. Patterns of variability were analysed using both morphometrics and inter simple sequence repeat (ISSR) data for P. reniforme (both subspecies), and P. sidoides. Results showed no support for the current subspecific division of P. reniforme but also no support for the current status of P. sidoides as a separate species. However, both morphometric and ISSR data show some evidence for the existence of two distinctly separate groups within P. reniforl1le subsp. velutinum as two distinct types have been recognized: those with bright pink flowers with long tubes (up to 70mm), and those with pale pink flowers with shorter tubes (as little as 8mm). These two forms have been found in a single population (Grahamstown), where they occur sympatrically, as well as in monomorphic populations. Analyses of the processes thought to be responsible for the observed patterns were conducted on the Grahamstown population. Observations of pollinators suggest that long-tongued insects (Butterflies, Nemestrinid flies) pollinate the long-tubed flowers and short-tongued insects (Bombyliid flies, long-tongued bees) pollinate short-tubed flowers. However, analysis of selection gradients in the population, indicate no directional selection is occurring for hypanthium lengths. The results of this thesis show that selection is occurring within Pelargonium reniforme, but at this time, selection is not strongly directional and floral differences are maintained, even when floral forms occur sympatrically. It is suggested that a review of the taxonomy be undertaken as well as additional pollination and selection studies to confirm suspected taxonomic groupings and relationships between the floral forms respectively. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: De Wet, Leigh-Ann Robynne
- Date: 2013-06-25
- Subjects: Pelargoniums -- Classification , Pelargoniums -- Pollen , Pelargoniums -- Selection
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4255 , http://hdl.handle.net/10962/d1008185 , Pelargoniums -- Classification , Pelargoniums -- Pollen , Pelargoniums -- Selection
- Description: Pelargonium reniforme is currently divided into two subspecies, P. reniforme subsp. reniforme and P. reniforme subsp. velutinum. The species falls into section Reniformia along with the closely-related P. sidoides. Observations of the section showed some discrepancies in the current taxonomy; mainly floral variation that was not recorded in the descriptions of the subspecies of P. reniforme, particularly the differences in hypanthium lengths. Patterns of variability were analysed using both morphometrics and inter simple sequence repeat (ISSR) data for P. reniforme (both subspecies), and P. sidoides. Results showed no support for the current subspecific division of P. reniforme but also no support for the current status of P. sidoides as a separate species. However, both morphometric and ISSR data show some evidence for the existence of two distinctly separate groups within P. reniforl1le subsp. velutinum as two distinct types have been recognized: those with bright pink flowers with long tubes (up to 70mm), and those with pale pink flowers with shorter tubes (as little as 8mm). These two forms have been found in a single population (Grahamstown), where they occur sympatrically, as well as in monomorphic populations. Analyses of the processes thought to be responsible for the observed patterns were conducted on the Grahamstown population. Observations of pollinators suggest that long-tongued insects (Butterflies, Nemestrinid flies) pollinate the long-tubed flowers and short-tongued insects (Bombyliid flies, long-tongued bees) pollinate short-tubed flowers. However, analysis of selection gradients in the population, indicate no directional selection is occurring for hypanthium lengths. The results of this thesis show that selection is occurring within Pelargonium reniforme, but at this time, selection is not strongly directional and floral differences are maintained, even when floral forms occur sympatrically. It is suggested that a review of the taxonomy be undertaken as well as additional pollination and selection studies to confirm suspected taxonomic groupings and relationships between the floral forms respectively. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
A systematic study of Berkheya and allies (Compositae)
- Authors: Phaliso, Ntombifikile
- Date: 2013
- Subjects: Compositae -- Africa, Southern , Daisies -- Africa, Southern , Compositae -- Phylogeny -- Africa, Southern , Compositae -- Geographical distribution -- Africa, Southern , Compositae -- Classification -- Africa, Southern , Cladistic analysis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4179 , http://hdl.handle.net/10962/d1003054
- Description: Berkheya Ehrh. is a genus of daisies in the tribe Arctotideae, subtribe Gorteriinae with over 80 species, most of which occur in southern Africa. This genus has centres of diversity associated with the montane regions of South Africa, including the Drakensberg Alpine Centre and Mpumalanga escarpment regions. Previous molecular and morphological studies indicate that Berkheya is paraphyletic. I present phylogenies based on nrDNA (ITS; Internal Transcribed Spacer) and cpDNA (psbA-trnH) sequence data analysed with Bayesian Inference and Parsimony. A phylogeny of combined cp- and nrDNA is also presented. These phylogenies are used to assess generic limits and to investigate the biogeographic patterns of Berkheya and its allies. The ITS phylogeny shows five well supported clades of Berkheya, two of which (Clades I and 2) are monophyletic summer rainfall region clades. Clades 3 to 5 are all paraphyletic winter rainfall clades with Cullumia occurring in the third clade, Cuspida occurring in the fourth and Didelta in Clade 5. Both psbA-trnH and combined phylogenies show concordance with the clade distribution shown in the ITS phylogeny. The ITS phylogeny was used to analyse correspondence with Roessler’s (1959) Series. It was found that the phylogeny showed considerable agreement with Roessler’s series, but B. bipinnatifida and B. spinosa of Series Speciosae may require some revision as well as taxa of monotypic series Cruciatae and Angustae. It is suggested that the latter series be merged with Cullumia species to form a single series. Some consideration should be taken to include Didelta species into Series Fruticosae as Didelta occurs in subclade 5b of Clade 5 with other Series Fruticosae taxa. Achene morphology was examined from species from each of the five clades to investigate the relationships of Berkheya and its allies, as well as to determine if there were any consistent achene features for each clade. The structure of surface cells on the fruit, the presence, absence and morphology of twin hairs as well as the structure of the pappus scales were found to be most useful in reflecting phylogenetic relationships within the clades. When compared with the clades of the ITS phylogeny, achene morphology showed consistent characters between taxa occurring in the same clades. As the most comprehensive study involving Berkheya, this phylogenenetic investigation was able to confirm that Berkheya is a paraphyletic genus with Didelta, Cullumia and Cuspida needing to be subsumed into Berkheya. An alternative classification is that taxa of Clade 5 could possibly be erected as an expanded Didelta, separate and sister to Berkheya.
- Full Text:
- Date Issued: 2013
- Authors: Phaliso, Ntombifikile
- Date: 2013
- Subjects: Compositae -- Africa, Southern , Daisies -- Africa, Southern , Compositae -- Phylogeny -- Africa, Southern , Compositae -- Geographical distribution -- Africa, Southern , Compositae -- Classification -- Africa, Southern , Cladistic analysis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4179 , http://hdl.handle.net/10962/d1003054
- Description: Berkheya Ehrh. is a genus of daisies in the tribe Arctotideae, subtribe Gorteriinae with over 80 species, most of which occur in southern Africa. This genus has centres of diversity associated with the montane regions of South Africa, including the Drakensberg Alpine Centre and Mpumalanga escarpment regions. Previous molecular and morphological studies indicate that Berkheya is paraphyletic. I present phylogenies based on nrDNA (ITS; Internal Transcribed Spacer) and cpDNA (psbA-trnH) sequence data analysed with Bayesian Inference and Parsimony. A phylogeny of combined cp- and nrDNA is also presented. These phylogenies are used to assess generic limits and to investigate the biogeographic patterns of Berkheya and its allies. The ITS phylogeny shows five well supported clades of Berkheya, two of which (Clades I and 2) are monophyletic summer rainfall region clades. Clades 3 to 5 are all paraphyletic winter rainfall clades with Cullumia occurring in the third clade, Cuspida occurring in the fourth and Didelta in Clade 5. Both psbA-trnH and combined phylogenies show concordance with the clade distribution shown in the ITS phylogeny. The ITS phylogeny was used to analyse correspondence with Roessler’s (1959) Series. It was found that the phylogeny showed considerable agreement with Roessler’s series, but B. bipinnatifida and B. spinosa of Series Speciosae may require some revision as well as taxa of monotypic series Cruciatae and Angustae. It is suggested that the latter series be merged with Cullumia species to form a single series. Some consideration should be taken to include Didelta species into Series Fruticosae as Didelta occurs in subclade 5b of Clade 5 with other Series Fruticosae taxa. Achene morphology was examined from species from each of the five clades to investigate the relationships of Berkheya and its allies, as well as to determine if there were any consistent achene features for each clade. The structure of surface cells on the fruit, the presence, absence and morphology of twin hairs as well as the structure of the pappus scales were found to be most useful in reflecting phylogenetic relationships within the clades. When compared with the clades of the ITS phylogeny, achene morphology showed consistent characters between taxa occurring in the same clades. As the most comprehensive study involving Berkheya, this phylogenenetic investigation was able to confirm that Berkheya is a paraphyletic genus with Didelta, Cullumia and Cuspida needing to be subsumed into Berkheya. An alternative classification is that taxa of Clade 5 could possibly be erected as an expanded Didelta, separate and sister to Berkheya.
- Full Text:
- Date Issued: 2013
Small mammal communities at high altitude within the Sneeuberg Mountain complex, Eastern Cape Province, South Africa
- Authors: Kok, Armand du Preez
- Date: 2012
- Subjects: Mountain animals -- Research -- South Africa -- Eastern Cape , Mammal populations -- Research -- South Africa -- Eastern Cape , Mammals -- Research -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5766 , http://hdl.handle.net/10962/d1005454 , Mountain animals -- Research -- South Africa -- Eastern Cape , Mammal populations -- Research -- South Africa -- Eastern Cape , Mammals -- Research -- South Africa -- Eastern Cape
- Description: Due to their widespread and specious nature, small mammals are ideal for biogeographical studies. Small mammals also effectively connect various trophic levels by being both consumers and prey items for other animals. The Great Escarpment is the dominant mountain landscape in South Africa. Yet, very little small mammal research has been conducted on the Great Escarpment outside of the Main Drakensberg Mountains. This is surprising given the importance of mountains in shaping regional ecology. In this study, I assessed the diversity and community composition of small mammals at three high altitude (>1700m) sites within the Sneeuberg Mountain Complex (SMC) from June 2009 to May 2010. I also tested the effectiveness of five different bait types for measuring small mammal diversity (i.e. number of individuals caught, species richness, Shannon diversity index and Simpson index of diversity). Out of a total of 423 captures, 292 individuals of 12 small mammal species (one shrew, one elephant shrew and 10 rodents) were recorded over 5280 trap nights. The species richness and diversity of small mammals captured at the three sites were similar and this homogeneity was probably related to the regional processes (e.g. climate and latitude) that govern species richness and diversity. The most effective bait type in terms of capture success, species richness and diversity measurements was peanut butter and oats. In addition, the use of richness estimators revealed that peanut butter and oats was the most effective bait for sampling the species richness of small mammals. The effectiveness of peanut butter and oats was related to this bait having a more attractive scent, when compared to the other bait types. Future studies should focus on researching the range of local and regional processes that drive small mammal diversity at high altitudes in South Africa. I also recommend the use of more than one bait type when planning to survey small mammal communities.
- Full Text:
- Date Issued: 2012
- Authors: Kok, Armand du Preez
- Date: 2012
- Subjects: Mountain animals -- Research -- South Africa -- Eastern Cape , Mammal populations -- Research -- South Africa -- Eastern Cape , Mammals -- Research -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5766 , http://hdl.handle.net/10962/d1005454 , Mountain animals -- Research -- South Africa -- Eastern Cape , Mammal populations -- Research -- South Africa -- Eastern Cape , Mammals -- Research -- South Africa -- Eastern Cape
- Description: Due to their widespread and specious nature, small mammals are ideal for biogeographical studies. Small mammals also effectively connect various trophic levels by being both consumers and prey items for other animals. The Great Escarpment is the dominant mountain landscape in South Africa. Yet, very little small mammal research has been conducted on the Great Escarpment outside of the Main Drakensberg Mountains. This is surprising given the importance of mountains in shaping regional ecology. In this study, I assessed the diversity and community composition of small mammals at three high altitude (>1700m) sites within the Sneeuberg Mountain Complex (SMC) from June 2009 to May 2010. I also tested the effectiveness of five different bait types for measuring small mammal diversity (i.e. number of individuals caught, species richness, Shannon diversity index and Simpson index of diversity). Out of a total of 423 captures, 292 individuals of 12 small mammal species (one shrew, one elephant shrew and 10 rodents) were recorded over 5280 trap nights. The species richness and diversity of small mammals captured at the three sites were similar and this homogeneity was probably related to the regional processes (e.g. climate and latitude) that govern species richness and diversity. The most effective bait type in terms of capture success, species richness and diversity measurements was peanut butter and oats. In addition, the use of richness estimators revealed that peanut butter and oats was the most effective bait for sampling the species richness of small mammals. The effectiveness of peanut butter and oats was related to this bait having a more attractive scent, when compared to the other bait types. Future studies should focus on researching the range of local and regional processes that drive small mammal diversity at high altitudes in South Africa. I also recommend the use of more than one bait type when planning to survey small mammal communities.
- Full Text:
- Date Issued: 2012
A taxonomic revision of the genera of the subtribe Dracophilinae (Aizoaceae: Ruschioideae)
- Authors: Mannheimer, Coleen Anne
- Date: 2006
- Subjects: Aizoaceae , Plants -- Classification , Cladistic analysis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4247 , http://hdl.handle.net/10962/d1007461 , Aizoaceae , Plants -- Classification , Cladistic analysis
- Description: Namibia, Juttadinteria and Dracophilus, the three genera belonging to the subtribe Dracophilinae Schwantes (Aizoaceae: Ruschioideae) were revised. Macro-morphology, leaf anatomy and micromorphology of leaf epidermides, pollen, tapetal orbicules and seed were studied in order to test taxon limits, to determine relationships between taxa, and to improve knowledge of their characteristics as well as the taxonomy of the subtribe. The investigation was based on herbarium specimens, field observations and cultivated, living plants. Phenetic cluster analyses were used to confirm species while intergeneric and interspecific relationships of the taxa so indicated were later tested by means of a phylogenetic analysis. The existence of the three genera was provisionally upheld by this study although phylogenetic analysis showed Namibia nested as a strongly supported monophyletic group within a poorly supported luttadinteria. Further work is needed to clarify whether Namibia should be sunk into Juttadinteria. The latest treatments of Juttadinteria and Dracophilus by Hartmann (2001) were supprted. However, in contrast to her latest treatment of Namibia, N. pomonae was sunk into N. cinerea and N. ponderosa was reinstated and typified. Juttadinteria was found to be a poorly resolved genus with many intergrading and overlapping characters, possible due to recent speciation. Possible subspecific groups within J. deserticola and J. simpsonii were indicated by the phenetic study but further work is needed before any formal infraspecific taxonomic rank can be assigned to them. Detailed descriptions of the nine elucidated species and the three genera as well as new keys for their identification are provided in order to facilitate further work in this group. Distribution maps are provided for the genera and species, and the subtribe. Variation of character states within the subtribe, distribution area, ecology and biology are discussed.
- Full Text:
- Date Issued: 2006
- Authors: Mannheimer, Coleen Anne
- Date: 2006
- Subjects: Aizoaceae , Plants -- Classification , Cladistic analysis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4247 , http://hdl.handle.net/10962/d1007461 , Aizoaceae , Plants -- Classification , Cladistic analysis
- Description: Namibia, Juttadinteria and Dracophilus, the three genera belonging to the subtribe Dracophilinae Schwantes (Aizoaceae: Ruschioideae) were revised. Macro-morphology, leaf anatomy and micromorphology of leaf epidermides, pollen, tapetal orbicules and seed were studied in order to test taxon limits, to determine relationships between taxa, and to improve knowledge of their characteristics as well as the taxonomy of the subtribe. The investigation was based on herbarium specimens, field observations and cultivated, living plants. Phenetic cluster analyses were used to confirm species while intergeneric and interspecific relationships of the taxa so indicated were later tested by means of a phylogenetic analysis. The existence of the three genera was provisionally upheld by this study although phylogenetic analysis showed Namibia nested as a strongly supported monophyletic group within a poorly supported luttadinteria. Further work is needed to clarify whether Namibia should be sunk into Juttadinteria. The latest treatments of Juttadinteria and Dracophilus by Hartmann (2001) were supprted. However, in contrast to her latest treatment of Namibia, N. pomonae was sunk into N. cinerea and N. ponderosa was reinstated and typified. Juttadinteria was found to be a poorly resolved genus with many intergrading and overlapping characters, possible due to recent speciation. Possible subspecific groups within J. deserticola and J. simpsonii were indicated by the phenetic study but further work is needed before any formal infraspecific taxonomic rank can be assigned to them. Detailed descriptions of the nine elucidated species and the three genera as well as new keys for their identification are provided in order to facilitate further work in this group. Distribution maps are provided for the genera and species, and the subtribe. Variation of character states within the subtribe, distribution area, ecology and biology are discussed.
- Full Text:
- Date Issued: 2006
Phylogeography and comparative ecophysiology of Chrysanthemoides Turn. Ex Medik. (Tribe Calenduleae)
- Authors: Howis, Seranne
- Date: 2005
- Subjects: Chrysanthemoides , Phylogeny , Ecophysiology , DNA , Plant genetics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4256 , http://hdl.handle.net/10962/d1008189
- Description: Chrysanthem Oides is a common Southern African shrub that grows in a variety of habitats. From coastal shrubland and fynbos to mountainous areas as far north as Kenya. The genus has two species and 8 subspecies. The diagnoses and delimitation of which have been based almost exclusively on morphological characteristics. This project aims to investigate, with the use of phylogenetic species concepts. The validity of these subspecies. Unlike biological species concepts that rely on reproductive isolation as a defining character of a species. Phylogenetic species concepts (PSC) are concerned with delimiting evolutionary significant units (ESUs). ESUs are evolutionarily isolated lineages, and under the PSC a species is an aggregation of organisms consistently diagnosable by a fixed character or combination of characters. This project therefore searched for genetic and physiological characters by which to delimit ESUs within the Cill), samhemoides genus. DNA sequencing was used to investigate the genetic characters, while gas exchange studies were used to investigate the ecophysiological characters. DNA sequence analysis indicated that the ESUs can be diagnosed by genetic means and that one species may be of hybrid origin. Field studies of three disparate genetically identifiable ESUs from three disparate climates found that there are noticeable differences in ecophysiological responses of these ESUs in the field. Plants from each ESU were transferred to a greenhouse and grown under identical conditions for several months and compared to determine if these traits are inherent, or elastic in relation to environmental conditions. Under simulated high rainfall conditions. There does not appear to be a significant difference in the photosynthetic traits.
- Full Text:
- Date Issued: 2005
Phylogeography and comparative ecophysiology of Chrysanthemoides Turn. Ex Medik. (Tribe Calenduleae)
- Authors: Howis, Seranne
- Date: 2005
- Subjects: Chrysanthemoides , Phylogeny , Ecophysiology , DNA , Plant genetics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4256 , http://hdl.handle.net/10962/d1008189
- Description: Chrysanthem Oides is a common Southern African shrub that grows in a variety of habitats. From coastal shrubland and fynbos to mountainous areas as far north as Kenya. The genus has two species and 8 subspecies. The diagnoses and delimitation of which have been based almost exclusively on morphological characteristics. This project aims to investigate, with the use of phylogenetic species concepts. The validity of these subspecies. Unlike biological species concepts that rely on reproductive isolation as a defining character of a species. Phylogenetic species concepts (PSC) are concerned with delimiting evolutionary significant units (ESUs). ESUs are evolutionarily isolated lineages, and under the PSC a species is an aggregation of organisms consistently diagnosable by a fixed character or combination of characters. This project therefore searched for genetic and physiological characters by which to delimit ESUs within the Cill), samhemoides genus. DNA sequencing was used to investigate the genetic characters, while gas exchange studies were used to investigate the ecophysiological characters. DNA sequence analysis indicated that the ESUs can be diagnosed by genetic means and that one species may be of hybrid origin. Field studies of three disparate genetically identifiable ESUs from three disparate climates found that there are noticeable differences in ecophysiological responses of these ESUs in the field. Plants from each ESU were transferred to a greenhouse and grown under identical conditions for several months and compared to determine if these traits are inherent, or elastic in relation to environmental conditions. Under simulated high rainfall conditions. There does not appear to be a significant difference in the photosynthetic traits.
- Full Text:
- Date Issued: 2005
Applied studies of some Southern African blowflies (Diptera: Calliphoridae) of forensic importance
- Authors: Lunt, Nicola
- Date: 2003
- Subjects: Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5803 , http://hdl.handle.net/10962/d1006202 , Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Description: Three major aspects of blowfly (Diptera: Calliphoridae) research were the focus of this study. Firstly, the phylogenetic relationships of 40 oestroid species from a variety of geographical localities were investigated using Cytochrome Oxidase b subunit I (COl) gene sequences. Maximum parsimony (MP) and Jukes-Cantor neighbor-joining (NJ) analyses both extracted a paraphyletic Calliphoridae, with the Calliphorinae-Luciliinae clade being sister to the Sarcophagidae. Short branch lengths within Chrysomya indicate a recent rapid radiation of this genus. Phormia and Protophormia either formed a sister clade to Chrysomya, or were embedded in this genus. Tree topologies were comparable between MP and NJ trees, but the positions of some genera were ambiguous. Secondly, developmental parameters and behaviour were investigated for four southern African species of forensically important blowflies viz. Chrysomya chloropyga, C. putoria, C. megacephala and Lucilia sericata, and ad hoc observations were made for Calliphora croceipalpis, Chrysomya marginalis and the predatory C. albiceps. Choice of oviposition substrate differed between species, mirroring substrate preferences in the field. Sexual dimorphism and dwarfism within a cohort complicated ageing maggots using size, but the use of developmental events (e.g. ecdysis) allowed ages to be determined unambiguously. Separate species status was supported for the previously synonymised C. chloropyga and C. putoria, by differences in maggot behaviour, larval growth rates and temperature optima. The proportion of total development time assigned to each larval instar and pupariation was variable among temperatures, but similar between congeneric species. Thirdly, since a negative linear relationship was found to occur between the developmental constant (K) and developmental zero (D₀) for both Calliphoridae and Sarcophagidae, the potential for predicting physiological parameters of unstudied taxa was investigated. Species and genera of Palaearctic origin generally had high K's and low D₀'s, and the reverse was true for the tropical taxa. It was found that both K and D₀ can be estimated for "unknown" taxa using the Felsenstein's Independent Contrasts (FIC) method of PDTree (Garland et al. 200 I), provided that branch lengths are relatively short and the phylogenetic position of the estimated taxon is unambiguous.
- Full Text:
- Date Issued: 2003
- Authors: Lunt, Nicola
- Date: 2003
- Subjects: Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5803 , http://hdl.handle.net/10962/d1006202 , Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Description: Three major aspects of blowfly (Diptera: Calliphoridae) research were the focus of this study. Firstly, the phylogenetic relationships of 40 oestroid species from a variety of geographical localities were investigated using Cytochrome Oxidase b subunit I (COl) gene sequences. Maximum parsimony (MP) and Jukes-Cantor neighbor-joining (NJ) analyses both extracted a paraphyletic Calliphoridae, with the Calliphorinae-Luciliinae clade being sister to the Sarcophagidae. Short branch lengths within Chrysomya indicate a recent rapid radiation of this genus. Phormia and Protophormia either formed a sister clade to Chrysomya, or were embedded in this genus. Tree topologies were comparable between MP and NJ trees, but the positions of some genera were ambiguous. Secondly, developmental parameters and behaviour were investigated for four southern African species of forensically important blowflies viz. Chrysomya chloropyga, C. putoria, C. megacephala and Lucilia sericata, and ad hoc observations were made for Calliphora croceipalpis, Chrysomya marginalis and the predatory C. albiceps. Choice of oviposition substrate differed between species, mirroring substrate preferences in the field. Sexual dimorphism and dwarfism within a cohort complicated ageing maggots using size, but the use of developmental events (e.g. ecdysis) allowed ages to be determined unambiguously. Separate species status was supported for the previously synonymised C. chloropyga and C. putoria, by differences in maggot behaviour, larval growth rates and temperature optima. The proportion of total development time assigned to each larval instar and pupariation was variable among temperatures, but similar between congeneric species. Thirdly, since a negative linear relationship was found to occur between the developmental constant (K) and developmental zero (D₀) for both Calliphoridae and Sarcophagidae, the potential for predicting physiological parameters of unstudied taxa was investigated. Species and genera of Palaearctic origin generally had high K's and low D₀'s, and the reverse was true for the tropical taxa. It was found that both K and D₀ can be estimated for "unknown" taxa using the Felsenstein's Independent Contrasts (FIC) method of PDTree (Garland et al. 200 I), provided that branch lengths are relatively short and the phylogenetic position of the estimated taxon is unambiguous.
- Full Text:
- Date Issued: 2003
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