Localisation of Theiler's Murine Encephalomyelitis virus non-structural proteins 2B, 2C, 2BC and 3A in BHK-21 cells, and the effect of amino acid substitutions in 2C on localisation and virus replication
- Authors: Murray, Lindsay
- Date: 2007
- Subjects: Encephalomyelitis -- Genetic aspects , Amino acid sequence , Picornaviruses , Viruses -- Reproduction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4090 , http://hdl.handle.net/10962/d1007722 , Encephalomyelitis -- Genetic aspects , Amino acid sequence , Picornaviruses , Viruses -- Reproduction
- Description: The picornavirus family includes significant human and animal viruses such as poliovirus (PV), human rhinovirus (HRV) and foot-and-mouth-disease virus (FMDV). Current disease treatment and control strategies are limited by an incomplete understanding of the interactions between the non-structural, replicative picornavirus proteins and host cell components. To investigate these interactions, Theiler's murine encephalomyelitis virus (TMEV) 2B, 2C, 2BC and 3A proteins were transiently expressed in BHK-21 cells and detected by indirect immunostaining and laser-scanning or epifluorescence microscopy. The signal of the 2B protein overlapped with that of the ER marker protein, ERp60, as well as that of the peripheral Golgi marker protein, β-COP. The 2C protein overlapped with ERp60 in a faint reticular stain, and localised to large punctate structures that partially overlapped with β-COP at higher levels of expression. The 2BC protein located to large perinuclear structures that overlapped exclusively with β-COP. The TMEV 3A protein signal overlapped with both ERp60 and β-COP stains, in addition in cells expressing the 3A protein the ER appeared swollen and bulbous while the Golgi was dispersed in some cells. 2C and 2BC proteins with C-terminal deletions localised in the same manner as the wild type proteins indicating that the localisation signals that determine subcellular localisation of the proteins are within the N-terminal 60 amino acids of the 2C protein. The significance of the high degree of conservation of the N-terminal domain of the 2C protein throughout the Picornaviridae was investigated through the introduction of amino acid substitution mutations at highly conserved residues in the N-terminal domain of 2C into the viral cDNA. Upon transfection of the viral RNA into BHK-21 cells, it was observed that substitution of amino acid residues 8, 18 and 29 abolished the ability of TMEV to induce cytopathic effect (CPE), while substitution of residues 4, 14 and 23 only attenuated the ability of TMEV to induce CPE. To determine whether amino acid substitution mutations would affect the localisation of the 2C protein, 2C proteins with substitution mutations at amino acids 4, 8, 14, 18, 23 and 29 were transiently expressed in BHK-21 cells and detected by indirect imrnunostaining and examination by laser-scanning confocal and epifluorescence microscopy. The 2C mutant 4, 8 and 29 proteins showed slightly altered localisation patterns compared to the wild type protein with a significant portion of the proteins localising in a perinuclear stain suggesting possible localisation to the nuclear envelop. The 2C mutant 14 and 18 proteins localised to a diffuse pattern in BHK-21 cells while the 2C mutant 23 protein located to small punctate structures that partially overlapped with the ERp60 stain but were completely separate from the β-COP stain. Finally, a hydrophilic, antigenic region of the 2C protein was expressed in frame with an N-terminal GST tag and was successfully purified on a pilot-scale and detected by Western analysis. This 2C178 peptide will be used to generate antibodies against the 2C and 2BC proteins for use in future studies. This study has furthered our knowledge of the localisation of the picornavirus 2B, 2C, 2BC and 3A proteins in host cells and identified a possible link between this localisation and an ability of TMEV to replicate in BHK-21 cells.
- Full Text:
- Authors: Murray, Lindsay
- Date: 2007
- Subjects: Encephalomyelitis -- Genetic aspects , Amino acid sequence , Picornaviruses , Viruses -- Reproduction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4090 , http://hdl.handle.net/10962/d1007722 , Encephalomyelitis -- Genetic aspects , Amino acid sequence , Picornaviruses , Viruses -- Reproduction
- Description: The picornavirus family includes significant human and animal viruses such as poliovirus (PV), human rhinovirus (HRV) and foot-and-mouth-disease virus (FMDV). Current disease treatment and control strategies are limited by an incomplete understanding of the interactions between the non-structural, replicative picornavirus proteins and host cell components. To investigate these interactions, Theiler's murine encephalomyelitis virus (TMEV) 2B, 2C, 2BC and 3A proteins were transiently expressed in BHK-21 cells and detected by indirect immunostaining and laser-scanning or epifluorescence microscopy. The signal of the 2B protein overlapped with that of the ER marker protein, ERp60, as well as that of the peripheral Golgi marker protein, β-COP. The 2C protein overlapped with ERp60 in a faint reticular stain, and localised to large punctate structures that partially overlapped with β-COP at higher levels of expression. The 2BC protein located to large perinuclear structures that overlapped exclusively with β-COP. The TMEV 3A protein signal overlapped with both ERp60 and β-COP stains, in addition in cells expressing the 3A protein the ER appeared swollen and bulbous while the Golgi was dispersed in some cells. 2C and 2BC proteins with C-terminal deletions localised in the same manner as the wild type proteins indicating that the localisation signals that determine subcellular localisation of the proteins are within the N-terminal 60 amino acids of the 2C protein. The significance of the high degree of conservation of the N-terminal domain of the 2C protein throughout the Picornaviridae was investigated through the introduction of amino acid substitution mutations at highly conserved residues in the N-terminal domain of 2C into the viral cDNA. Upon transfection of the viral RNA into BHK-21 cells, it was observed that substitution of amino acid residues 8, 18 and 29 abolished the ability of TMEV to induce cytopathic effect (CPE), while substitution of residues 4, 14 and 23 only attenuated the ability of TMEV to induce CPE. To determine whether amino acid substitution mutations would affect the localisation of the 2C protein, 2C proteins with substitution mutations at amino acids 4, 8, 14, 18, 23 and 29 were transiently expressed in BHK-21 cells and detected by indirect imrnunostaining and examination by laser-scanning confocal and epifluorescence microscopy. The 2C mutant 4, 8 and 29 proteins showed slightly altered localisation patterns compared to the wild type protein with a significant portion of the proteins localising in a perinuclear stain suggesting possible localisation to the nuclear envelop. The 2C mutant 14 and 18 proteins localised to a diffuse pattern in BHK-21 cells while the 2C mutant 23 protein located to small punctate structures that partially overlapped with the ERp60 stain but were completely separate from the β-COP stain. Finally, a hydrophilic, antigenic region of the 2C protein was expressed in frame with an N-terminal GST tag and was successfully purified on a pilot-scale and detected by Western analysis. This 2C178 peptide will be used to generate antibodies against the 2C and 2BC proteins for use in future studies. This study has furthered our knowledge of the localisation of the picornavirus 2B, 2C, 2BC and 3A proteins in host cells and identified a possible link between this localisation and an ability of TMEV to replicate in BHK-21 cells.
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Measuring the RFI environment of the South African SKA site
- Authors: Manners, Paul John
- Date: 2007
- Subjects: Radio telescopes , Radio telescopes -- South Africa , Radio astronomy , Radio astronomy -- South Africa , Square Kilometer Array (Spacecraft) , Radio -- Interference -- Measurement
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5474 , http://hdl.handle.net/10962/d1005259 , Radio telescopes , Radio telescopes -- South Africa , Radio astronomy , Radio astronomy -- South Africa , Square Kilometer Array (Spacecraft) , Radio -- Interference -- Measurement
- Description: The Square Kilometre Array (SKA) Project is an international effort to build the world’s largest radio telescope. It will be 100 times more sensitive than any other radio telescope currently in existence and will consist of thousands of dishes placed at baselines up to 3000 km. In addition to its increased sensitivity it will operate over a very wide frequency range (current specification is 100 MHz - 22 GHz) and will use frequency bands not primarily allocated to radio astronomy. Because of this the telescope needs to be located at a site with low levels of radio frequency interference (RFI). This implies a site that is remote and away from human activity. In bidding to host the SKA, South Africa was required to conduct an RFI survey at its proposed site for a period of 12 months. Apart from this core site, where more than half the SKA dishes may potentially be deployed, the measurement of remote sites in Southern Africa was also required. To conduct measurements at these sites, three mobile measurement systems were designed and built by the South African SKA Project. The design considerations, implementation and RFI measurements recorded during this campaign will be the focus for this dissertation.
- Full Text:
- Authors: Manners, Paul John
- Date: 2007
- Subjects: Radio telescopes , Radio telescopes -- South Africa , Radio astronomy , Radio astronomy -- South Africa , Square Kilometer Array (Spacecraft) , Radio -- Interference -- Measurement
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5474 , http://hdl.handle.net/10962/d1005259 , Radio telescopes , Radio telescopes -- South Africa , Radio astronomy , Radio astronomy -- South Africa , Square Kilometer Array (Spacecraft) , Radio -- Interference -- Measurement
- Description: The Square Kilometre Array (SKA) Project is an international effort to build the world’s largest radio telescope. It will be 100 times more sensitive than any other radio telescope currently in existence and will consist of thousands of dishes placed at baselines up to 3000 km. In addition to its increased sensitivity it will operate over a very wide frequency range (current specification is 100 MHz - 22 GHz) and will use frequency bands not primarily allocated to radio astronomy. Because of this the telescope needs to be located at a site with low levels of radio frequency interference (RFI). This implies a site that is remote and away from human activity. In bidding to host the SKA, South Africa was required to conduct an RFI survey at its proposed site for a period of 12 months. Apart from this core site, where more than half the SKA dishes may potentially be deployed, the measurement of remote sites in Southern Africa was also required. To conduct measurements at these sites, three mobile measurement systems were designed and built by the South African SKA Project. The design considerations, implementation and RFI measurements recorded during this campaign will be the focus for this dissertation.
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Particle precipitation effects on the South African ionosphere
- Authors: Sibanda, Patrick
- Date: 2007
- Subjects: Ionosphere -- South Africa , Precipitation (Chemistry) -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5481 , http://hdl.handle.net/10962/d1005267 , Ionosphere -- South Africa , Precipitation (Chemistry) -- South Africa
- Description: Particle precipitation involves the injection of energetic particles into the ionosphere which could increase the ionisation and conductivity of the upper atmosphere. The goal of this study was to examine the ionospheric response and changes due to particle precipitation in the region over South Africa, using a combination of groundbased and satellite instruments. Particle precipitation events were identified from satellite particle flux measurements of the Defence Meteorological Satellite Program (DMSP). Comprehensive studies were done on the events of 5 April, 2000 and 7 October, 2000. Analysis of the data from the satellite instruments indicates that no particle precipitation was observed over the South African region during these events and that it is unlikely to occur during other such events. To validate the data, methods and tools used in this study, precipitation in the South Atlantic anomaly (SAA) region is used. Satellite ion density measurements revealed that strong density enhancements occurred over the SAA region at satellite altitudes during the precipitation events, but this did not occur in the South African region. The measurements also revealed how the ionisation enhancements in the SAA region correlated with geomagnetic and solar activities. Particle precipitation and convective electric fields are two major magnetospheric energy sources to the upper atmosphere in the auroral and the SAA regions. These increase dramatically during geomagnetic storms and can disturb thermospheric circulation in the atmosphere and alter the rates of production and recombination of the ionised species. Ionosonde observations at Grahamstown, South Africa (33.30S, 26.50E), provided the data to build a picture of the response of the ionosphere over the South African region to particle precipitation during the precipitation events. This analysis showed that, within the confines of the available data, no direct connections between particle precipitation events and disturbances in the ionosphere over this region were revealed.
- Full Text:
- Authors: Sibanda, Patrick
- Date: 2007
- Subjects: Ionosphere -- South Africa , Precipitation (Chemistry) -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5481 , http://hdl.handle.net/10962/d1005267 , Ionosphere -- South Africa , Precipitation (Chemistry) -- South Africa
- Description: Particle precipitation involves the injection of energetic particles into the ionosphere which could increase the ionisation and conductivity of the upper atmosphere. The goal of this study was to examine the ionospheric response and changes due to particle precipitation in the region over South Africa, using a combination of groundbased and satellite instruments. Particle precipitation events were identified from satellite particle flux measurements of the Defence Meteorological Satellite Program (DMSP). Comprehensive studies were done on the events of 5 April, 2000 and 7 October, 2000. Analysis of the data from the satellite instruments indicates that no particle precipitation was observed over the South African region during these events and that it is unlikely to occur during other such events. To validate the data, methods and tools used in this study, precipitation in the South Atlantic anomaly (SAA) region is used. Satellite ion density measurements revealed that strong density enhancements occurred over the SAA region at satellite altitudes during the precipitation events, but this did not occur in the South African region. The measurements also revealed how the ionisation enhancements in the SAA region correlated with geomagnetic and solar activities. Particle precipitation and convective electric fields are two major magnetospheric energy sources to the upper atmosphere in the auroral and the SAA regions. These increase dramatically during geomagnetic storms and can disturb thermospheric circulation in the atmosphere and alter the rates of production and recombination of the ionised species. Ionosonde observations at Grahamstown, South Africa (33.30S, 26.50E), provided the data to build a picture of the response of the ionosphere over the South African region to particle precipitation during the precipitation events. This analysis showed that, within the confines of the available data, no direct connections between particle precipitation events and disturbances in the ionosphere over this region were revealed.
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Population structure, growth and recruitment of two exploited infralittoral molluscs (Haliotis midae and Turbo sarmaticus) along the south east coast, South Africa
- Authors: Proudfoot, Lee-Anne
- Date: 2007
- Subjects: Abalones -- South Africa , Abalones -- Growth -- South Africa , Turbinidae -- South Africa , Mollusks -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5744 , http://hdl.handle.net/10962/d1005430 , Abalones -- South Africa , Abalones -- Growth -- South Africa , Turbinidae -- South Africa , Mollusks -- South Africa
- Description: The two most frequently exploited species along the south east coast of South Africa are the gastropods, Haliotis midae (abalone) and Turbo sarmaticus (alikreukel). H. midae is a high valued commercial species, and suffers intense levels of illegal fishing. T. sarmaticus however, has no commercial value but is the preferred food item for impoverished subsistence communities. Owing to the fact that no legal commercial fishery exists for either species along the south coast, very few studies have been undertaken, especially in the heavily exploited infralittoral. Infralittoral size frequency distributions for both species revealed significant variation in density and size among sites of varying exploitation pressure. Densities ranged between 0 – 2.23 m⁻² (H. midae) and 0.03 – 4.93 m⁻² (T. sarmaticus) and maximum shell lengths ranged from 49.4 – 153.5 mm (H. midae) and 28.3 – 104.4 mm (T. sarmaticus). Relatively high densities and large sizes were found in marine reserves and secluded areas, and low densities and small sizes at sites near to large population centres and within the former Ciskei homeland region. Mean size of the largest 10% of the population, total density and sexually mature density were significantly related to exploitation predictors for both species. In addition, densities of H. midae juveniles were significantly related to exploitation predictors, suggesting that recruitment may be suppressed at the most exploited sites. Exploitation of T. sarmaticus tended to be localized with refuge and subtidal populations persisting. H. midae exploitation was however, far more extensive and intense. Growth of H. midae was investigated using three methods; mark-recapture, cohort analysis and growth banding analysis at Kowie Rocks, Port Alfred. The most useful of these methods for determining growth was a new technique described for growth banding analysis; which was validated using cohort analysis and measurements of shells of known age. This technique was less time consuming and labour intensive than previously described methods. Abalone growth was best described by the Schnute (1981) growth function. Systematic geographic variation in growth was observed for 10 sites along the South African coastline. Significant differences in growth among sites existed for animals between 0-4 years (P < 0.0001) and 4-6 years (P < 0.0001), and in the mean maximum sizes attained (P < 0.001). In general, abalone from the south east/east coast were found to have faster growth rates, smaller mean maximum sizes and attained sexual maturity earlier than those along the south west/ west coast. Haliotis midae recruit and juvenile densities were found to differ significantly among sites of varying exploitation pressure (P < 0.0001) and among months for recruit densities (P < 0.001). Exploited sites had low recruit and juvenile densities compared to unexploited sites and peak recruitment occurred during October/ November 2005. Recruit densities were significantly related to infralittoral adult densities during two of the three sampling months (P<0.05), when recruitment was low. No relationship was observed during the period of high recruitment, with all sites receiving high recruit densities. It was concluded that variation in recruit densities was the result of a combination of both density-dependent relationships (i.e. local spawner density and temporal variability in recruitment intensity) and the possible dispersal capabilities of H. midae. In addition, it was concluded that at present recruitment overfishing was not occurring along the south east coast. Post-recruitment mortality rates were variable but relatively constant, with hypothetical percentage survival and density curves revealing high rates and similar mortality curves among sites. Variation in juvenile densities was consequently a result of initial recruit densities and not variation in post-recruitment mortality. T. sarmaticus populations were found to be regionally sustainable and persisted along the south east coast due to adjacent intertidal and subtidal refuge populations. However, H. midae populations are becoming decimated along the south east coast. From the information obtained in this study new management proposals were suggested and discussed, such as closed areas and region-based management fisheries together with stock enhancement. These suggestions may prove to be feasible alternatives to present management strategies.
- Full Text:
- Authors: Proudfoot, Lee-Anne
- Date: 2007
- Subjects: Abalones -- South Africa , Abalones -- Growth -- South Africa , Turbinidae -- South Africa , Mollusks -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5744 , http://hdl.handle.net/10962/d1005430 , Abalones -- South Africa , Abalones -- Growth -- South Africa , Turbinidae -- South Africa , Mollusks -- South Africa
- Description: The two most frequently exploited species along the south east coast of South Africa are the gastropods, Haliotis midae (abalone) and Turbo sarmaticus (alikreukel). H. midae is a high valued commercial species, and suffers intense levels of illegal fishing. T. sarmaticus however, has no commercial value but is the preferred food item for impoverished subsistence communities. Owing to the fact that no legal commercial fishery exists for either species along the south coast, very few studies have been undertaken, especially in the heavily exploited infralittoral. Infralittoral size frequency distributions for both species revealed significant variation in density and size among sites of varying exploitation pressure. Densities ranged between 0 – 2.23 m⁻² (H. midae) and 0.03 – 4.93 m⁻² (T. sarmaticus) and maximum shell lengths ranged from 49.4 – 153.5 mm (H. midae) and 28.3 – 104.4 mm (T. sarmaticus). Relatively high densities and large sizes were found in marine reserves and secluded areas, and low densities and small sizes at sites near to large population centres and within the former Ciskei homeland region. Mean size of the largest 10% of the population, total density and sexually mature density were significantly related to exploitation predictors for both species. In addition, densities of H. midae juveniles were significantly related to exploitation predictors, suggesting that recruitment may be suppressed at the most exploited sites. Exploitation of T. sarmaticus tended to be localized with refuge and subtidal populations persisting. H. midae exploitation was however, far more extensive and intense. Growth of H. midae was investigated using three methods; mark-recapture, cohort analysis and growth banding analysis at Kowie Rocks, Port Alfred. The most useful of these methods for determining growth was a new technique described for growth banding analysis; which was validated using cohort analysis and measurements of shells of known age. This technique was less time consuming and labour intensive than previously described methods. Abalone growth was best described by the Schnute (1981) growth function. Systematic geographic variation in growth was observed for 10 sites along the South African coastline. Significant differences in growth among sites existed for animals between 0-4 years (P < 0.0001) and 4-6 years (P < 0.0001), and in the mean maximum sizes attained (P < 0.001). In general, abalone from the south east/east coast were found to have faster growth rates, smaller mean maximum sizes and attained sexual maturity earlier than those along the south west/ west coast. Haliotis midae recruit and juvenile densities were found to differ significantly among sites of varying exploitation pressure (P < 0.0001) and among months for recruit densities (P < 0.001). Exploited sites had low recruit and juvenile densities compared to unexploited sites and peak recruitment occurred during October/ November 2005. Recruit densities were significantly related to infralittoral adult densities during two of the three sampling months (P<0.05), when recruitment was low. No relationship was observed during the period of high recruitment, with all sites receiving high recruit densities. It was concluded that variation in recruit densities was the result of a combination of both density-dependent relationships (i.e. local spawner density and temporal variability in recruitment intensity) and the possible dispersal capabilities of H. midae. In addition, it was concluded that at present recruitment overfishing was not occurring along the south east coast. Post-recruitment mortality rates were variable but relatively constant, with hypothetical percentage survival and density curves revealing high rates and similar mortality curves among sites. Variation in juvenile densities was consequently a result of initial recruit densities and not variation in post-recruitment mortality. T. sarmaticus populations were found to be regionally sustainable and persisted along the south east coast due to adjacent intertidal and subtidal refuge populations. However, H. midae populations are becoming decimated along the south east coast. From the information obtained in this study new management proposals were suggested and discussed, such as closed areas and region-based management fisheries together with stock enhancement. These suggestions may prove to be feasible alternatives to present management strategies.
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Pricing exotic options using C++
- Authors: Nhongo, Tawuya D R
- Date: 2007
- Subjects: C++ (Computer program language) , Monte Carlo method , Simulation methods , Options (Finance) -- Mathematical models , Pricing -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5577 , http://hdl.handle.net/10962/d1008373 , C++ (Computer program language) , Monte Carlo method , Simulation methods , Options (Finance) -- Mathematical models , Pricing -- Mathematical models
- Description: This document demonstrates the use of the C++ programming language as a simulation tool in the efficient pricing of exotic European options. Extensions to the basic problem of simulation pricing are undertaken including variance reduction by conditional expectation, control and antithetic variates. Ultimately we were able to produce a modularized, easily extend-able program which effectively makes use of Monte Carlo simulation techniques to price lookback, Asian and barrier exotic options. Theories of variance reduction were validated except in cases where we used control variates in combination with the other variance reduction techniques in which case we observed increased variance. Again, the main aim of this half thesis was to produce a C++ program which would produce stable pricings of exotic options.
- Full Text:
- Authors: Nhongo, Tawuya D R
- Date: 2007
- Subjects: C++ (Computer program language) , Monte Carlo method , Simulation methods , Options (Finance) -- Mathematical models , Pricing -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5577 , http://hdl.handle.net/10962/d1008373 , C++ (Computer program language) , Monte Carlo method , Simulation methods , Options (Finance) -- Mathematical models , Pricing -- Mathematical models
- Description: This document demonstrates the use of the C++ programming language as a simulation tool in the efficient pricing of exotic European options. Extensions to the basic problem of simulation pricing are undertaken including variance reduction by conditional expectation, control and antithetic variates. Ultimately we were able to produce a modularized, easily extend-able program which effectively makes use of Monte Carlo simulation techniques to price lookback, Asian and barrier exotic options. Theories of variance reduction were validated except in cases where we used control variates in combination with the other variance reduction techniques in which case we observed increased variance. Again, the main aim of this half thesis was to produce a C++ program which would produce stable pricings of exotic options.
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Securing media streams in an Asterisk-based environment and evaluating the resulting performance cost
- Authors: Clayton, Bradley
- Date: 2007 , 2007-01-08
- Subjects: Asterisk (Computer file) , Computer networks -- Security measures , Internet telephony -- Security measures
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4647 , http://hdl.handle.net/10962/d1006606 , Asterisk (Computer file) , Computer networks -- Security measures , Internet telephony -- Security measures
- Description: When adding Confidentiality, Integrity and Availability (CIA) to a multi-user VoIP (Voice over IP) system, performance and quality are at risk. The aim of this study is twofold. Firstly, it describes current methods suitable to secure voice streams within a VoIP system and make them available in an Asterisk-based VoIP environment. (Asterisk is a well established, open-source, TDM/VoIP PBX.) Secondly, this study evaluates the performance cost incurred after implementing each security method within the Asterisk-based system, using a special testbed suite, named DRAPA, which was developed expressly for this study. The three security methods implemented and studied were IPSec (Internet Protocol Security), SRTP (Secure Real-time Transport Protocol), and SIAX2 (Secure Inter-Asterisk eXchange 2 protocol). From the experiments, it was found that bandwidth and CPU usage were significantly affected by the addition of CIA. In ranking the three security methods in terms of these two resources, it was found that SRTP incurs the least bandwidth overhead, followed by SIAX2 and then IPSec. Where CPU utilisation is concerned, it was found that SIAX2 incurs the least overhead, followed by IPSec, and then SRTP.
- Full Text:
- Authors: Clayton, Bradley
- Date: 2007 , 2007-01-08
- Subjects: Asterisk (Computer file) , Computer networks -- Security measures , Internet telephony -- Security measures
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4647 , http://hdl.handle.net/10962/d1006606 , Asterisk (Computer file) , Computer networks -- Security measures , Internet telephony -- Security measures
- Description: When adding Confidentiality, Integrity and Availability (CIA) to a multi-user VoIP (Voice over IP) system, performance and quality are at risk. The aim of this study is twofold. Firstly, it describes current methods suitable to secure voice streams within a VoIP system and make them available in an Asterisk-based VoIP environment. (Asterisk is a well established, open-source, TDM/VoIP PBX.) Secondly, this study evaluates the performance cost incurred after implementing each security method within the Asterisk-based system, using a special testbed suite, named DRAPA, which was developed expressly for this study. The three security methods implemented and studied were IPSec (Internet Protocol Security), SRTP (Secure Real-time Transport Protocol), and SIAX2 (Secure Inter-Asterisk eXchange 2 protocol). From the experiments, it was found that bandwidth and CPU usage were significantly affected by the addition of CIA. In ranking the three security methods in terms of these two resources, it was found that SRTP incurs the least bandwidth overhead, followed by SIAX2 and then IPSec. Where CPU utilisation is concerned, it was found that SIAX2 incurs the least overhead, followed by IPSec, and then SRTP.
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Securing softswitches from malicious attacks
- Authors: Opie, Jake Weyman
- Date: 2007
- Subjects: Internet telephony -- Security measures , Computer networks -- Security measures , Digital telephone systems , Communication -- Technological innovations , Computer network protocols , TCP/IP (Computer network protocol) , Switching theory
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4683 , http://hdl.handle.net/10962/d1007714 , Internet telephony -- Security measures , Computer networks -- Security measures , Digital telephone systems , Communication -- Technological innovations , Computer network protocols , TCP/IP (Computer network protocol) , Switching theory
- Description: Traditionally, real-time communication, such as voice calls, has run on separate, closed networks. Of all the limitations that these networks had, the ability of malicious attacks to cripple communication was not a crucial one. This situation has changed radically now that real-time communication and data have merged to share the same network. The objective of this project is to investigate the securing of softswitches with functionality similar to Private Branch Exchanges (PBX) from malicious attacks. The focus of the project will be a practical investigation of how to secure ILANGA, an ASTERISK-based system under development at Rhodes University. The practical investigation that focuses on ILANGA is based on performing six varied experiments on the different components of ILANGA. Before the six experiments are performed, basic preliminary security measures and the restrictions placed on the access to the database are discussed. The outcomes of these experiments are discussed and the precise reasons why these attacks were either successful or unsuccessful are given. Suggestions of a theoretical nature on how to defend against the successful attacks are also presented.
- Full Text:
- Authors: Opie, Jake Weyman
- Date: 2007
- Subjects: Internet telephony -- Security measures , Computer networks -- Security measures , Digital telephone systems , Communication -- Technological innovations , Computer network protocols , TCP/IP (Computer network protocol) , Switching theory
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4683 , http://hdl.handle.net/10962/d1007714 , Internet telephony -- Security measures , Computer networks -- Security measures , Digital telephone systems , Communication -- Technological innovations , Computer network protocols , TCP/IP (Computer network protocol) , Switching theory
- Description: Traditionally, real-time communication, such as voice calls, has run on separate, closed networks. Of all the limitations that these networks had, the ability of malicious attacks to cripple communication was not a crucial one. This situation has changed radically now that real-time communication and data have merged to share the same network. The objective of this project is to investigate the securing of softswitches with functionality similar to Private Branch Exchanges (PBX) from malicious attacks. The focus of the project will be a practical investigation of how to secure ILANGA, an ASTERISK-based system under development at Rhodes University. The practical investigation that focuses on ILANGA is based on performing six varied experiments on the different components of ILANGA. Before the six experiments are performed, basic preliminary security measures and the restrictions placed on the access to the database are discussed. The outcomes of these experiments are discussed and the precise reasons why these attacks were either successful or unsuccessful are given. Suggestions of a theoretical nature on how to defend against the successful attacks are also presented.
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Statistical analyses of artificial waterpoints: their effect on the herbaceous and woody structure composition within the Kruger National Park
- Authors: Goodall, Victoria Lucy
- Date: 2007
- Subjects: South African National Parks , Ecology -- Statistical methods , Regression analysis , Log-linear models , Game reserves -- South Africa , Kruger National Park (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5570 , http://hdl.handle.net/10962/d1002810 , South African National Parks , Ecology -- Statistical methods , Regression analysis , Log-linear models , Game reserves -- South Africa , Kruger National Park (South Africa)
- Description: The objective of this project is to link the statistical theory used in the ecological sciences with an actual project that was developed for the South African National Parks Scientific Services. It investigates the changes that have occurred in the herbaceous and woody structure due to the closure of artificial waterpoints; including the impacts that elephants and other herbivores have on the vegetation of the Kruger National Park. This project was designed in conjunction with South African National Parks (SANP) Scientific Services and it is a registered project with this department. The results of this project will be submitted to Scientific Services in accordance with the terms and conditions of a SANP research project. A major concern within the KNP is the declining numbers of rare antelope and numerous projects have been developed to investigate possible ways of halting this decline and thus protecting the heterogeneity of the Kruger National Park. Three different datasets were investigated, covering three aspects of vegetation structure and composition within the KNP. The first investigated the changes that have occurred since the N'washitsumbe enclosure in the Far Northern KNP was fenced off from the rest of the park. The results show that over the 40 years since the enclosure was built, changes have occurred which have resulted in a significant difference in the abundance of Increaser 2 and Decreaser grass species between the inside and the outside of the enclosure. Increaser 2 and Decreaser categories are the result of a grass species classification depending on whether the species thrives or is depressed by heavy grazing. The difference in grass species composition and structure between the inside and the outside of the enclosure indicates that the grazing animals within the KNP have influenced the grass composition in a way that favours the dominant animals. This has resulted in a declining roan antelope population - one of the species that is considered as a 'rare antelope'. Many artificial waterpoints (boreholes and dams) have also been closed throughout the KNP in the hope of resulting in a change in vegetation structure and composition in favour of the roan. Veld condition assessment data for 87 boreholes throughout the Park was analyzed to determine whether the veld in the vicinity is beginning to change towards a more Decreaser dominated sward which would favour the roan. The results were analyzed for the different regions of the Park; and they indicate that changes are becoming evident; however, the results are not particularly conclusive, yet. The majority of the boreholes were closed between 1994 and 1998 which means that not a lot of data were available to be analyzed. A similar study conducted in another 10 years time might reveal more meaningful results. However the results are moving in the direction hoped for by the management of the KNP. The results show that the grass composition has a higher proportion of Decreaser grasses since the closure of the waterpoints, and the grass biomass around these areas has also improved. The results were analyzed on an individual basis; and then on a regional basis as the minimal data meant that the individual analyses did not provide any significant results. A third study was then done on the impact that the rapidly increasing elephant population on the vegetation within the Riparian zone along three rivers in the Far Northern region of the KNP. The riparian zone is an important part of the landscape, in terms of providing food for many animals as well as shade. The elephant population has increased substantially since the termination of the culling program and this means that the feeding requirements of the population has increased which could result in severe damage upon the vegetation, as elephants can be very destructive feeders. The results show surprising differences between the three years of data that were analyzed; however the results indicate that the elephants are targeting specific height ranges of trees when feeding; however they do not seem to consistently target specific tree species. This is positive for the diversity of the Riparian zone as this region is very important both ecologically and aesthetically for the tourists who visit the Park.
- Full Text:
- Authors: Goodall, Victoria Lucy
- Date: 2007
- Subjects: South African National Parks , Ecology -- Statistical methods , Regression analysis , Log-linear models , Game reserves -- South Africa , Kruger National Park (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5570 , http://hdl.handle.net/10962/d1002810 , South African National Parks , Ecology -- Statistical methods , Regression analysis , Log-linear models , Game reserves -- South Africa , Kruger National Park (South Africa)
- Description: The objective of this project is to link the statistical theory used in the ecological sciences with an actual project that was developed for the South African National Parks Scientific Services. It investigates the changes that have occurred in the herbaceous and woody structure due to the closure of artificial waterpoints; including the impacts that elephants and other herbivores have on the vegetation of the Kruger National Park. This project was designed in conjunction with South African National Parks (SANP) Scientific Services and it is a registered project with this department. The results of this project will be submitted to Scientific Services in accordance with the terms and conditions of a SANP research project. A major concern within the KNP is the declining numbers of rare antelope and numerous projects have been developed to investigate possible ways of halting this decline and thus protecting the heterogeneity of the Kruger National Park. Three different datasets were investigated, covering three aspects of vegetation structure and composition within the KNP. The first investigated the changes that have occurred since the N'washitsumbe enclosure in the Far Northern KNP was fenced off from the rest of the park. The results show that over the 40 years since the enclosure was built, changes have occurred which have resulted in a significant difference in the abundance of Increaser 2 and Decreaser grass species between the inside and the outside of the enclosure. Increaser 2 and Decreaser categories are the result of a grass species classification depending on whether the species thrives or is depressed by heavy grazing. The difference in grass species composition and structure between the inside and the outside of the enclosure indicates that the grazing animals within the KNP have influenced the grass composition in a way that favours the dominant animals. This has resulted in a declining roan antelope population - one of the species that is considered as a 'rare antelope'. Many artificial waterpoints (boreholes and dams) have also been closed throughout the KNP in the hope of resulting in a change in vegetation structure and composition in favour of the roan. Veld condition assessment data for 87 boreholes throughout the Park was analyzed to determine whether the veld in the vicinity is beginning to change towards a more Decreaser dominated sward which would favour the roan. The results were analyzed for the different regions of the Park; and they indicate that changes are becoming evident; however, the results are not particularly conclusive, yet. The majority of the boreholes were closed between 1994 and 1998 which means that not a lot of data were available to be analyzed. A similar study conducted in another 10 years time might reveal more meaningful results. However the results are moving in the direction hoped for by the management of the KNP. The results show that the grass composition has a higher proportion of Decreaser grasses since the closure of the waterpoints, and the grass biomass around these areas has also improved. The results were analyzed on an individual basis; and then on a regional basis as the minimal data meant that the individual analyses did not provide any significant results. A third study was then done on the impact that the rapidly increasing elephant population on the vegetation within the Riparian zone along three rivers in the Far Northern region of the KNP. The riparian zone is an important part of the landscape, in terms of providing food for many animals as well as shade. The elephant population has increased substantially since the termination of the culling program and this means that the feeding requirements of the population has increased which could result in severe damage upon the vegetation, as elephants can be very destructive feeders. The results show surprising differences between the three years of data that were analyzed; however the results indicate that the elephants are targeting specific height ranges of trees when feeding; however they do not seem to consistently target specific tree species. This is positive for the diversity of the Riparian zone as this region is very important both ecologically and aesthetically for the tourists who visit the Park.
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Studies towards the development of novel multidentate ligands
- Authors: Magqi, Nceba
- Date: 2007
- Subjects: Density functionals , Ligands , Ligands -- Design , Ligands -- Analysis , Camphor
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4358 , http://hdl.handle.net/10962/d1005023 , Density functionals , Ligands , Ligands -- Design , Ligands -- Analysis , Camphor
- Description: In this study, attention has been given to the design and synthesis of novel multidentate ligands for use in the construction of ruthenium-based metathesis catalysts, and their chelating potential has been explored by computer modelling at the Density Functional Theory (DFT) level. Both Kemp’s triacid (1,3,5-trimethyl-1,3,5-cyclohexanetricarboxylic acid) and D-(+)-camphor have been investigated as molecular scaffolds for the development of such ligands. However selective elaboration of the functional groups in Kemp’s triacid proved difficult to achieve, and the research has focused on the development of camphor derivatives. The synthesis of the camphor-based ligands has involved C-8 functionalisation and ring-opening of the bicyclic system to afford tridentate products. The formation of 9-iodocamphorquinone bis(ethylene ketal) together with the desired product, the 8-iodo isomer, has been confirmed by single crystal X-ray analysis of both compounds. Formation of the 9-iodo analogue has provided new insights into the intramolecular rearrangement of camphor skeleton, and the mechanistic implications have been assessed by coset analysis. Attempts to effect nucleophilic displacement of the 8-halogeno groups by nucleophilic donor moieties proved unexpectedly difficult and, coupled with the susceptibility of the carbonyl groups to nucleophilic attack, has led to the formation of novel tricyclic products, viz., 1,6-dimethyl-3-(2-pyridylamino)-4-oxatricyclo[4.3.0.0[superscript 3,7]]-2-nonanone and 6,7-dimethyl-3-(2-pyridylamino)-4-oxatricyclo -[4.3.0.0[superscript 3,7]]-2-nonanone. However the diphenylphosphine group was successfully introduced at C-8 and oxidative ring-opening of the camphor skeleton has afforded the tridentate ligands, 2-(diphenylphosphinoylmethyl)-1,2-dimethyl-1,3-cyclopentanedicarboxylic acid and 2-(diphenylphosphinoylmethyl)-1,3-bis(hydroxymethyl)1,2-dimethylcyclopentane. One- and two-dimensional NMR and, where appropriate, high-resolution MS methods have been used to characterise the products. Three [superscript 13]C NMR chemical shift prediction programmes, viz., ChemWindow and the MODGRAPH neural network and HOSE (Hierachially Ordered Spherical description of Environment), have been applied to representative compounds to assess their efficacy. While the predicted shifts correlated reasonably well with the experimental data, they proved to be insufficiently accurate to differentiate the isomeric systems examined.
- Full Text:
- Authors: Magqi, Nceba
- Date: 2007
- Subjects: Density functionals , Ligands , Ligands -- Design , Ligands -- Analysis , Camphor
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4358 , http://hdl.handle.net/10962/d1005023 , Density functionals , Ligands , Ligands -- Design , Ligands -- Analysis , Camphor
- Description: In this study, attention has been given to the design and synthesis of novel multidentate ligands for use in the construction of ruthenium-based metathesis catalysts, and their chelating potential has been explored by computer modelling at the Density Functional Theory (DFT) level. Both Kemp’s triacid (1,3,5-trimethyl-1,3,5-cyclohexanetricarboxylic acid) and D-(+)-camphor have been investigated as molecular scaffolds for the development of such ligands. However selective elaboration of the functional groups in Kemp’s triacid proved difficult to achieve, and the research has focused on the development of camphor derivatives. The synthesis of the camphor-based ligands has involved C-8 functionalisation and ring-opening of the bicyclic system to afford tridentate products. The formation of 9-iodocamphorquinone bis(ethylene ketal) together with the desired product, the 8-iodo isomer, has been confirmed by single crystal X-ray analysis of both compounds. Formation of the 9-iodo analogue has provided new insights into the intramolecular rearrangement of camphor skeleton, and the mechanistic implications have been assessed by coset analysis. Attempts to effect nucleophilic displacement of the 8-halogeno groups by nucleophilic donor moieties proved unexpectedly difficult and, coupled with the susceptibility of the carbonyl groups to nucleophilic attack, has led to the formation of novel tricyclic products, viz., 1,6-dimethyl-3-(2-pyridylamino)-4-oxatricyclo[4.3.0.0[superscript 3,7]]-2-nonanone and 6,7-dimethyl-3-(2-pyridylamino)-4-oxatricyclo -[4.3.0.0[superscript 3,7]]-2-nonanone. However the diphenylphosphine group was successfully introduced at C-8 and oxidative ring-opening of the camphor skeleton has afforded the tridentate ligands, 2-(diphenylphosphinoylmethyl)-1,2-dimethyl-1,3-cyclopentanedicarboxylic acid and 2-(diphenylphosphinoylmethyl)-1,3-bis(hydroxymethyl)1,2-dimethylcyclopentane. One- and two-dimensional NMR and, where appropriate, high-resolution MS methods have been used to characterise the products. Three [superscript 13]C NMR chemical shift prediction programmes, viz., ChemWindow and the MODGRAPH neural network and HOSE (Hierachially Ordered Spherical description of Environment), have been applied to representative compounds to assess their efficacy. While the predicted shifts correlated reasonably well with the experimental data, they proved to be insufficiently accurate to differentiate the isomeric systems examined.
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Study of metallophthalocyanines attached onto pre-modified gold surfaces
- Authors: Mashazi, Philani Nkosinathi
- Date: 2007
- Subjects: Phthalocyanines , Electrochemistry , Electrodes, Enzyme , Glucose -- Measurement
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4361 , http://hdl.handle.net/10962/d1005026 , Phthalocyanines , Electrochemistry , Electrodes, Enzyme , Glucose -- Measurement
- Description: Tetra-carboxy acid chloride phthalocyanine complexes of cobalt, iron and manganese were synthesized and characterized using spectroscopic and electrochemical techniques. These complexes were fabricated as thin films on gold electrode following a covalent immobilization and self-assembling methods. Surface electrochemical and spectroscopic characterization showed that these complexes are surface-confined species. The characterization using spectroscopic and electrochemical methods confirmed the formation of thiol and MPc SAMs on gold electrode. The electrocatalytic behaviour of the SAM modified gold electrodes was studied for the detection of L-cysteine and hydrogen peroxide. The limits of detection (LoD) for Lcysteine were of the orders of 10[superscript -7] mol.L[superscript -1] for all the MPc complexes studied and the LoD for hydrogen peroxide at cobalt phthalocyanine modified gold electrode was of the orders of 10[superscript -7]mol.L[superscript -1] for both electrocatalytic oxidation and reduction. The modification process for gold electrodes was reproducible and showed good stability, if stored in pH 4 phosphate buffer solutions and can be used over a long period of time. The cobalt phthalocyanine modified gold electrode was also investigated for the fabrication of glucose oxidase (GOx)-based biosensor and as an electron mediator between the enzyme and gold electrode. The behaviour of the enzyme modified gold electrode towards the detection of glucose was studied and the results gave a limit of detection of the orders of 10[superscript -6] mol.L[superscript -1] with low binding constant (4.8 mM) of enzyme (GOx) to substrate (glucose) referred to as Michaelis-Menten constant. The practical applications, i.e. the real sample analysis and interference studies, for the enzyme modified gold electrodes were investigated. These studies showed that the enzyme electrode is valuable and can be used for glucose detection.
- Full Text:
- Authors: Mashazi, Philani Nkosinathi
- Date: 2007
- Subjects: Phthalocyanines , Electrochemistry , Electrodes, Enzyme , Glucose -- Measurement
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4361 , http://hdl.handle.net/10962/d1005026 , Phthalocyanines , Electrochemistry , Electrodes, Enzyme , Glucose -- Measurement
- Description: Tetra-carboxy acid chloride phthalocyanine complexes of cobalt, iron and manganese were synthesized and characterized using spectroscopic and electrochemical techniques. These complexes were fabricated as thin films on gold electrode following a covalent immobilization and self-assembling methods. Surface electrochemical and spectroscopic characterization showed that these complexes are surface-confined species. The characterization using spectroscopic and electrochemical methods confirmed the formation of thiol and MPc SAMs on gold electrode. The electrocatalytic behaviour of the SAM modified gold electrodes was studied for the detection of L-cysteine and hydrogen peroxide. The limits of detection (LoD) for Lcysteine were of the orders of 10[superscript -7] mol.L[superscript -1] for all the MPc complexes studied and the LoD for hydrogen peroxide at cobalt phthalocyanine modified gold electrode was of the orders of 10[superscript -7]mol.L[superscript -1] for both electrocatalytic oxidation and reduction. The modification process for gold electrodes was reproducible and showed good stability, if stored in pH 4 phosphate buffer solutions and can be used over a long period of time. The cobalt phthalocyanine modified gold electrode was also investigated for the fabrication of glucose oxidase (GOx)-based biosensor and as an electron mediator between the enzyme and gold electrode. The behaviour of the enzyme modified gold electrode towards the detection of glucose was studied and the results gave a limit of detection of the orders of 10[superscript -6] mol.L[superscript -1] with low binding constant (4.8 mM) of enzyme (GOx) to substrate (glucose) referred to as Michaelis-Menten constant. The practical applications, i.e. the real sample analysis and interference studies, for the enzyme modified gold electrodes were investigated. These studies showed that the enzyme electrode is valuable and can be used for glucose detection.
- Full Text:
Synthesis of novel coumarin derivatives as potential inhibitors of HIV-1 protease
- Authors: Rose, Nathan Rolf
- Date: 2007 , 2013-07-01
- Subjects: Coumarins , Protease Inhibitors , Heterocyclic compounds -- Derivatives , HIV infections -- Treatment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4430 , http://hdl.handle.net/10962/d1007220 , Coumarins , Protease Inhibitors , Heterocyclic compounds -- Derivatives , HIV infections -- Treatment
- Description: This research has focused on the development of novel coumann derivatives containing peptide-like side chains as potential HIV-1 protease inhibitors. The reaction of various salicylaldehyde derivatives with tert-butyl acrylate In the presence of 1,4- diazabicyclo[2.2.2]octane (DABCO) has afforded a series of Baylis-Hillman adducts in moderate yield. Cyclisation of the adducts in the presence of HCI afforded the corresponding 3-(chloromethyl)coumarin derivatives, which have been reacted with various amine hydrochlorides in the presence of Proton Sponge® to afford a series of novel 3- (aminomethyl)coumarin derivatives, which were fully characterised by NMR and HRMS methods. Various approaches to the introduction of hydroxyl or amino groups at the C-4 position of coumarin and the 3-(chloromethyl)coumarin derivatives have been explored; these have included dihydroxylation of the coumarin double bond, and the synthesis of 4- benzylaminocoumarin derivatives as potential intermediates. The Vilsmeier-Haack and Mannich reactions have also been investigated as possible methods of introducing the desired peptide-like functionality. Computer modelling of selected structures has indicated that some of the novel 3- (aminomethyl)coumarin derivatives may exhibit activity as inhibitors of HIV-1 protease. The planned enzyme inhibition assays were unfortunately precluded by the aqueous insolubility of the selected compounds. Three ¹³C NMR chemical shift algorithms, viz., Modgraph Neural Network, Modgraph HOSE and Chern Window, have been applied to selected compounds prepared in this study. The Modgraph Neural Network algorithm was found, in all cases, to provide the most accurate correlations with the experimentally-determined chemical shifts. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Rose, Nathan Rolf
- Date: 2007 , 2013-07-01
- Subjects: Coumarins , Protease Inhibitors , Heterocyclic compounds -- Derivatives , HIV infections -- Treatment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4430 , http://hdl.handle.net/10962/d1007220 , Coumarins , Protease Inhibitors , Heterocyclic compounds -- Derivatives , HIV infections -- Treatment
- Description: This research has focused on the development of novel coumann derivatives containing peptide-like side chains as potential HIV-1 protease inhibitors. The reaction of various salicylaldehyde derivatives with tert-butyl acrylate In the presence of 1,4- diazabicyclo[2.2.2]octane (DABCO) has afforded a series of Baylis-Hillman adducts in moderate yield. Cyclisation of the adducts in the presence of HCI afforded the corresponding 3-(chloromethyl)coumarin derivatives, which have been reacted with various amine hydrochlorides in the presence of Proton Sponge® to afford a series of novel 3- (aminomethyl)coumarin derivatives, which were fully characterised by NMR and HRMS methods. Various approaches to the introduction of hydroxyl or amino groups at the C-4 position of coumarin and the 3-(chloromethyl)coumarin derivatives have been explored; these have included dihydroxylation of the coumarin double bond, and the synthesis of 4- benzylaminocoumarin derivatives as potential intermediates. The Vilsmeier-Haack and Mannich reactions have also been investigated as possible methods of introducing the desired peptide-like functionality. Computer modelling of selected structures has indicated that some of the novel 3- (aminomethyl)coumarin derivatives may exhibit activity as inhibitors of HIV-1 protease. The planned enzyme inhibition assays were unfortunately precluded by the aqueous insolubility of the selected compounds. Three ¹³C NMR chemical shift algorithms, viz., Modgraph Neural Network, Modgraph HOSE and Chern Window, have been applied to selected compounds prepared in this study. The Modgraph Neural Network algorithm was found, in all cases, to provide the most accurate correlations with the experimentally-determined chemical shifts. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
Synthesis of triprenylated toluquinone and toluhydroquinone metabolites from a marine-derived Penicillium fungus
- Authors: Scheepers, Brent Ashley
- Date: 2007
- Subjects: Penicillium , Antineoplastic agents , Marine fungi , Quinone
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4373 , http://hdl.handle.net/10962/d1005038 , Penicillium , Antineoplastic agents , Marine fungi , Quinone
- Description: This project forms part of a collaborative effort between the marine natural products chemists at Rhodes University and the medical biochemists at the University of Cape Town’s School of Medicine. Our UCT collaborators tested the cytotoxicity of a group of toluhydroquinones and toluquinones (9-15) against the oesophageal cancer cell line WHCO1 and revealed that the triprenylated toluhydroquinone 11 and it’s oxidised analogue 12 were the most active. This thesis presents an investigation into the role of the polyprenyl side-chain in the cytotoxicity of compound 11 and it’s oxidised analogue 12 by synthesizing and testing the cytotoxicity of simplified analogues of this compound. The synthesis of the two ortho-prenylated toluhydroquinone analogues 5-methyl-2-[(2'E,6'E)-3',7' -dimethyl-2',6'-octadienyl]-1,4-benzenediol (19) and 5-methyl-2-[(2'E,6'E)-3',7',11'-trimethyl-2',6',10'-dodecatrienyl]-1,4-benzenediol (21) and their two ortho-prenylated toluquinone analogues, 5-methyl-2-[(2'E,6'E)-3',7'-dimethyl-2',6'-octadienyl]-2,5-cyclohexadiene-1,4-dione (20) and 5-methyl-2-[(2'E,6'E)-3',7',11'-trimethyl-2',6',10'-dodecatrienyl]-2,5-cyclohexadiene-1,4-dione (22) is described. Our initial attempts to couple geranyl bromide, farnesyl bromide and farnesal to the aromatic precursors m-cresol and 1,4-dimethoxy-2-methylbenzene using directed ortho-prenylation and phenoxide carbon-alkylation were unsuccessful. The four target analogues were eventually synthesized via the initial metal halogen exchange reaction between 1-bromo-2,5-dimethoxy-4-methylbenzene and geranyl bromide/farnesyl bromide using n-BuLi and TMEDA in ditheyl ether at 0 °C to yield 92 and 104 respectively in moderate yield. The demethylation of both compounds preceded smoothly using AgO giving the target analogues 20 and 22 in good yield (approx. 90 %). The reduction of quinones 20 and 22 with sodium dithionite gave 19 and 21 in quantitative yield. The synthesis reported here is the first regioselective synthesis of these compounds. The anti-oesophageal cancer activity of 19-22 and two commercially available non-prenylated analogues 17 and 18 were tested against WHCO1. The conclusion drawn from the anti-oesophageal cancer study was that the polyprenyl side-chain plays a negligable role in the cytotoxicity of compounds such as 11 and 9 against the oesophageal cancer cell line WHCO1.
- Full Text:
- Authors: Scheepers, Brent Ashley
- Date: 2007
- Subjects: Penicillium , Antineoplastic agents , Marine fungi , Quinone
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4373 , http://hdl.handle.net/10962/d1005038 , Penicillium , Antineoplastic agents , Marine fungi , Quinone
- Description: This project forms part of a collaborative effort between the marine natural products chemists at Rhodes University and the medical biochemists at the University of Cape Town’s School of Medicine. Our UCT collaborators tested the cytotoxicity of a group of toluhydroquinones and toluquinones (9-15) against the oesophageal cancer cell line WHCO1 and revealed that the triprenylated toluhydroquinone 11 and it’s oxidised analogue 12 were the most active. This thesis presents an investigation into the role of the polyprenyl side-chain in the cytotoxicity of compound 11 and it’s oxidised analogue 12 by synthesizing and testing the cytotoxicity of simplified analogues of this compound. The synthesis of the two ortho-prenylated toluhydroquinone analogues 5-methyl-2-[(2'E,6'E)-3',7' -dimethyl-2',6'-octadienyl]-1,4-benzenediol (19) and 5-methyl-2-[(2'E,6'E)-3',7',11'-trimethyl-2',6',10'-dodecatrienyl]-1,4-benzenediol (21) and their two ortho-prenylated toluquinone analogues, 5-methyl-2-[(2'E,6'E)-3',7'-dimethyl-2',6'-octadienyl]-2,5-cyclohexadiene-1,4-dione (20) and 5-methyl-2-[(2'E,6'E)-3',7',11'-trimethyl-2',6',10'-dodecatrienyl]-2,5-cyclohexadiene-1,4-dione (22) is described. Our initial attempts to couple geranyl bromide, farnesyl bromide and farnesal to the aromatic precursors m-cresol and 1,4-dimethoxy-2-methylbenzene using directed ortho-prenylation and phenoxide carbon-alkylation were unsuccessful. The four target analogues were eventually synthesized via the initial metal halogen exchange reaction between 1-bromo-2,5-dimethoxy-4-methylbenzene and geranyl bromide/farnesyl bromide using n-BuLi and TMEDA in ditheyl ether at 0 °C to yield 92 and 104 respectively in moderate yield. The demethylation of both compounds preceded smoothly using AgO giving the target analogues 20 and 22 in good yield (approx. 90 %). The reduction of quinones 20 and 22 with sodium dithionite gave 19 and 21 in quantitative yield. The synthesis reported here is the first regioselective synthesis of these compounds. The anti-oesophageal cancer activity of 19-22 and two commercially available non-prenylated analogues 17 and 18 were tested against WHCO1. The conclusion drawn from the anti-oesophageal cancer study was that the polyprenyl side-chain plays a negligable role in the cytotoxicity of compounds such as 11 and 9 against the oesophageal cancer cell line WHCO1.
- Full Text:
The design and assessment of an integrated municipal waste beneficiation facility : towards improved sewage sludge management in developing countries
- Authors: Keirungi, Juliana
- Date: 2007
- Subjects: Sewage -- Purification -- South Africa -- Eastern Cape , Sewage sludge -- South Africa -- Eastern Cape -- Management , Water quality management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4755 , http://hdl.handle.net/10962/d1007138 , Sewage -- Purification -- South Africa -- Eastern Cape , Sewage sludge -- South Africa -- Eastern Cape -- Management , Water quality management -- South Africa -- Eastern Cape
- Description: This research aimed to firstly confirm the hypothesis that the current management of sewage sludge generated during the treatment of wastewater in the Eastern Cape Province of South Africa is poor and, as such, this material poses a threat to local environmental and human health. Secondly, through a rational design approach, the current research also aimed to design and assess the suitability of a novel integrated process incorporating appropriate technologies for improved sewage sludge management. The lack of an appropriate tool for the assessment of the integrated process necessitated the development of an appropriate technology assessment (TA) methodology based on environmental, technical, social and economic criteria. A combination of informal participatory methods such as semi-structured interviews and formal research methods including questionnaires, risk assessment exercises and laboratory analyses were used. Based on the above it was estimated that 116 tons dry sludge were generated in the province per day and that the concentration of heavy metals present in the sludge was generally within the limits for reuse on agricultural land. Furthermore the sludge from all sample sewage treatment works (STWs) was found to be free of any detectable pathogens. Despite the above, a preliminary risk assessment and chemical analysis revealed that existing sludge management practices at sample STWs posed a threat to the environment, particularly to water resources and exacerbated the problems associated with the discharge of poorly treated municipal wastewater. The root causes of the poor sludge management were considered to be a lack of non-regulatory incentives and financial resources. Highly integrated ecologically engineered systems were thought to offer a solution to the dual problem of poor sludge management and municipal effluent treatment, while providing necessary economic incentives. To facilitate the design of a system appropriate to local conditions, it was necessary to first develop a rational design methodology, which incorporated a detailed TA step. The result of the design process was an Integrated Waste Beneficiation Facility (IWBF) that incorporated a number of process units comprised of appropriate technologies including composting, vermicomposting, algal ponding technology and aquaculture. A detailed TA indicated that the benefits of the proposed IWBF would, at the majority of sample sites, outweigh the potential negative impacts and it was thus recommended that investigations should continue on pilot-scale facilities. Furthermore, although the proposed TA based on four sustainability criteria categories was thought to provide a more accurate assessment of the true sustainability of a technology, the acquisition of information was problematic highlighting the need to re-think current TA methodologies and to address associated constraints allowing the tool to be used and fully comprehensive.
- Full Text:
- Authors: Keirungi, Juliana
- Date: 2007
- Subjects: Sewage -- Purification -- South Africa -- Eastern Cape , Sewage sludge -- South Africa -- Eastern Cape -- Management , Water quality management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4755 , http://hdl.handle.net/10962/d1007138 , Sewage -- Purification -- South Africa -- Eastern Cape , Sewage sludge -- South Africa -- Eastern Cape -- Management , Water quality management -- South Africa -- Eastern Cape
- Description: This research aimed to firstly confirm the hypothesis that the current management of sewage sludge generated during the treatment of wastewater in the Eastern Cape Province of South Africa is poor and, as such, this material poses a threat to local environmental and human health. Secondly, through a rational design approach, the current research also aimed to design and assess the suitability of a novel integrated process incorporating appropriate technologies for improved sewage sludge management. The lack of an appropriate tool for the assessment of the integrated process necessitated the development of an appropriate technology assessment (TA) methodology based on environmental, technical, social and economic criteria. A combination of informal participatory methods such as semi-structured interviews and formal research methods including questionnaires, risk assessment exercises and laboratory analyses were used. Based on the above it was estimated that 116 tons dry sludge were generated in the province per day and that the concentration of heavy metals present in the sludge was generally within the limits for reuse on agricultural land. Furthermore the sludge from all sample sewage treatment works (STWs) was found to be free of any detectable pathogens. Despite the above, a preliminary risk assessment and chemical analysis revealed that existing sludge management practices at sample STWs posed a threat to the environment, particularly to water resources and exacerbated the problems associated with the discharge of poorly treated municipal wastewater. The root causes of the poor sludge management were considered to be a lack of non-regulatory incentives and financial resources. Highly integrated ecologically engineered systems were thought to offer a solution to the dual problem of poor sludge management and municipal effluent treatment, while providing necessary economic incentives. To facilitate the design of a system appropriate to local conditions, it was necessary to first develop a rational design methodology, which incorporated a detailed TA step. The result of the design process was an Integrated Waste Beneficiation Facility (IWBF) that incorporated a number of process units comprised of appropriate technologies including composting, vermicomposting, algal ponding technology and aquaculture. A detailed TA indicated that the benefits of the proposed IWBF would, at the majority of sample sites, outweigh the potential negative impacts and it was thus recommended that investigations should continue on pilot-scale facilities. Furthermore, although the proposed TA based on four sustainability criteria categories was thought to provide a more accurate assessment of the true sustainability of a technology, the acquisition of information was problematic highlighting the need to re-think current TA methodologies and to address associated constraints allowing the tool to be used and fully comprehensive.
- Full Text:
The effect of elephants (Loxodonta africana, Blumenbach, 1797) on Xeric Succulent Thicket
- Authors: Knott, Edward Joseph
- Date: 2007
- Subjects: Addo Elephant National Park (South Africa) , African elephant , Elephants -- South Africa -- Eastern Cape , Elephants -- Nutrition -- South Africa -- Eastern Cape , Elephants -- Ecology -- South Africa -- Eastern Cape , Elephants -- Behavior -- South Africa -- Eastern Cape , Succulent plants -- South Africa -- Eastern Cape , Woody plants -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5674 , http://hdl.handle.net/10962/d1005359 , Addo Elephant National Park (South Africa) , African elephant , Elephants -- South Africa -- Eastern Cape , Elephants -- Nutrition -- South Africa -- Eastern Cape , Elephants -- Ecology -- South Africa -- Eastern Cape , Elephants -- Behavior -- South Africa -- Eastern Cape , Succulent plants -- South Africa -- Eastern Cape , Woody plants -- South Africa -- Eastern Cape
- Description: This study looks at the impact of elephant feeding on the Xeric Succulent Thicket component of Eastern Cape Subtropical Thicket (ECST) in Addo Elephant National Park (AENP). Observations of elephant feeding were carried out and vegetation transects were surveyed for impact of elephant feeding. The results indicated that the Nyati elephants spent the majority of their time grazing (nearly 90%), particularly the cow-young herds, and especially when the herd gathered in larger numbers. Browsing events were concentrated on Acacia karroo (81%) and there was no significant difference between the sexes in their preference for this species. Despite being subjected to most of the browsing, the majority of A. karroo trees were undamaged and the effect of elephants was generally light. It appears unlikely that, three years after re-introduction to Nyati, the elephants have had an effect on community structure of the vegetation. Surveys were conducted on stands of the alien invasive weed prickly pear Opuntia ficus-indica, and it was recorded that elephants in Nyati have had a dramatic effect on prickly pear, utilising all adult plants assessed and destroying 70% of them. This level of destruction in such a short period of time suggests that prickly pear is a highly favoured species. The results from the present study suggest that elephants can play a role in the control of prickly pear. Results are discussed in terms of elephants as both megaherbivores and keystone species, and as agents of intermediate disturbance.
- Full Text:
- Authors: Knott, Edward Joseph
- Date: 2007
- Subjects: Addo Elephant National Park (South Africa) , African elephant , Elephants -- South Africa -- Eastern Cape , Elephants -- Nutrition -- South Africa -- Eastern Cape , Elephants -- Ecology -- South Africa -- Eastern Cape , Elephants -- Behavior -- South Africa -- Eastern Cape , Succulent plants -- South Africa -- Eastern Cape , Woody plants -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5674 , http://hdl.handle.net/10962/d1005359 , Addo Elephant National Park (South Africa) , African elephant , Elephants -- South Africa -- Eastern Cape , Elephants -- Nutrition -- South Africa -- Eastern Cape , Elephants -- Ecology -- South Africa -- Eastern Cape , Elephants -- Behavior -- South Africa -- Eastern Cape , Succulent plants -- South Africa -- Eastern Cape , Woody plants -- South Africa -- Eastern Cape
- Description: This study looks at the impact of elephant feeding on the Xeric Succulent Thicket component of Eastern Cape Subtropical Thicket (ECST) in Addo Elephant National Park (AENP). Observations of elephant feeding were carried out and vegetation transects were surveyed for impact of elephant feeding. The results indicated that the Nyati elephants spent the majority of their time grazing (nearly 90%), particularly the cow-young herds, and especially when the herd gathered in larger numbers. Browsing events were concentrated on Acacia karroo (81%) and there was no significant difference between the sexes in their preference for this species. Despite being subjected to most of the browsing, the majority of A. karroo trees were undamaged and the effect of elephants was generally light. It appears unlikely that, three years after re-introduction to Nyati, the elephants have had an effect on community structure of the vegetation. Surveys were conducted on stands of the alien invasive weed prickly pear Opuntia ficus-indica, and it was recorded that elephants in Nyati have had a dramatic effect on prickly pear, utilising all adult plants assessed and destroying 70% of them. This level of destruction in such a short period of time suggests that prickly pear is a highly favoured species. The results from the present study suggest that elephants can play a role in the control of prickly pear. Results are discussed in terms of elephants as both megaherbivores and keystone species, and as agents of intermediate disturbance.
- Full Text:
The effect of geography, cultivation and harvest technique on the umckalin concentration and growth of pelargonium sidoides (Geraniaceae)
- Authors: White, Andrew Graeme
- Date: 2007
- Subjects: Pelargoniums , Geraniaceae , Botany -- South Africa -- Eastern Cape , Traditional medicine -- South Africa -- Eastern Cape , Medicinal plants -- South Africa -- Eastern Cape , Coumarins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4234 , http://hdl.handle.net/10962/d1003803 , Pelargoniums , Geraniaceae , Botany -- South Africa -- Eastern Cape , Traditional medicine -- South Africa -- Eastern Cape , Medicinal plants -- South Africa -- Eastern Cape , Coumarins
- Description: Pelargonium sidoides DC. (Geraniaceae) root extracts are used in the Eastern Cape Province of South Africa as a traditional medicine for the treatment of respiratory tract and gastro-intestinal infections. Ethanolic extracts are used globally as herbal treatments for bronchitis, asthma and as an immune system booster. Despite documented exploitation of wild populations by illegal harvesters, this species has not been awarded a protected status. The high level of harvest in the years preceding this study prompted this investigation of the prospects for sustainable root harvest through wild harvest and greenhouse cultivation. A novel method was developed for the purification of umckalin, a bioactive constituent in root extracts, such that the root umckalin concentrations of wild and cultivated plants could be quantified by HPLC. As part of the cultivation experiments, the concentration of umckalin in roots was measured for plants across part of the species’ distribution range in the Eastern Cape Province. This survey revealed that root umckalin concentrations were inversely related to the average annual rainfall of the collection site (r² = 0.94, p = 0.007) and directly related to soil pH (r² = 0.97, p = 0.002). Thus, the possibility of inducing high umckalin concentrations in greenhouse-cultivated plants was investigated by subjecting plants to rapid and prolonged water stress treatments. Two leaf applied hormone treatments (cytokinin and gibberellin) and a root competition treatment with a fast growing annual (Conyza albida) were also investigated based on the potential function of umckalin in P. sidoides plants. These five treatments did not significantly affect root umckalin concentrations compared to well-watered controls. The results of further experiments suggested that umckalin production may have been influenced by the geographical origin and genetics of plants rather than environmental variation. Following wild harvest experiments, the regrowth of replanted shoots from which a standard proportion of the root was harvested showed that water availability affected shoot survival but not root regrowth rate. Regrowth rates were low, questioning the viability of wild harvest. In contrast, greenhouse cultivated plants showed ca. six times greater growth rates, supporting the cultivation of roots to supply future market demand.
- Full Text:
- Authors: White, Andrew Graeme
- Date: 2007
- Subjects: Pelargoniums , Geraniaceae , Botany -- South Africa -- Eastern Cape , Traditional medicine -- South Africa -- Eastern Cape , Medicinal plants -- South Africa -- Eastern Cape , Coumarins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4234 , http://hdl.handle.net/10962/d1003803 , Pelargoniums , Geraniaceae , Botany -- South Africa -- Eastern Cape , Traditional medicine -- South Africa -- Eastern Cape , Medicinal plants -- South Africa -- Eastern Cape , Coumarins
- Description: Pelargonium sidoides DC. (Geraniaceae) root extracts are used in the Eastern Cape Province of South Africa as a traditional medicine for the treatment of respiratory tract and gastro-intestinal infections. Ethanolic extracts are used globally as herbal treatments for bronchitis, asthma and as an immune system booster. Despite documented exploitation of wild populations by illegal harvesters, this species has not been awarded a protected status. The high level of harvest in the years preceding this study prompted this investigation of the prospects for sustainable root harvest through wild harvest and greenhouse cultivation. A novel method was developed for the purification of umckalin, a bioactive constituent in root extracts, such that the root umckalin concentrations of wild and cultivated plants could be quantified by HPLC. As part of the cultivation experiments, the concentration of umckalin in roots was measured for plants across part of the species’ distribution range in the Eastern Cape Province. This survey revealed that root umckalin concentrations were inversely related to the average annual rainfall of the collection site (r² = 0.94, p = 0.007) and directly related to soil pH (r² = 0.97, p = 0.002). Thus, the possibility of inducing high umckalin concentrations in greenhouse-cultivated plants was investigated by subjecting plants to rapid and prolonged water stress treatments. Two leaf applied hormone treatments (cytokinin and gibberellin) and a root competition treatment with a fast growing annual (Conyza albida) were also investigated based on the potential function of umckalin in P. sidoides plants. These five treatments did not significantly affect root umckalin concentrations compared to well-watered controls. The results of further experiments suggested that umckalin production may have been influenced by the geographical origin and genetics of plants rather than environmental variation. Following wild harvest experiments, the regrowth of replanted shoots from which a standard proportion of the root was harvested showed that water availability affected shoot survival but not root regrowth rate. Regrowth rates were low, questioning the viability of wild harvest. In contrast, greenhouse cultivated plants showed ca. six times greater growth rates, supporting the cultivation of roots to supply future market demand.
- Full Text:
The effects of selected reference toxicants on embryonic development of the freshwater shrimp caridina nilotica (Decapoda: Atyidae)
- Authors: Ketse, Noziphiwo
- Date: 2007
- Subjects: Toxicity testing -- South Africa , Shrimp fisheries -- South Africa , Decapoda , Caridina , Water quality management -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6025 , http://hdl.handle.net/10962/d1005367 , Toxicity testing -- South Africa , Shrimp fisheries -- South Africa , Decapoda , Caridina , Water quality management -- South Africa
- Description: Aquatic toxicity tests are increasingly being used in water resource management worldwide, and currently in South Africa, policy and legislation are being drafted to reflect this international trend. While standard toxicity test methods and test organisms are being considered to develop and set water quality guidelines and effluent discharge limits, it is not clear whether guidelines and discharge limits set using these standard test organisms will be sufficient to protect South Africa’s scarce water resources. As part of ongoing research to investigate the use of indigenous riverine organisms as toxicity test organisms a number of potential species have been identified, including the freshwater shrimp Caridina nilotica. For much of the history of aquatic toxicological data the bulk of the data has been generated by acute toxicity testing, based on short exposures and using mortality as the response end point. There are relatively few chronic, longterm tests with sub-lethal endpoints. However, it was recognized that information about longer exposure durations and non lethal response endpoints was needed, instead of mortality. Chronic tests can provide a more environmentally realistic measure of chemical toxicity than acute toxicity tests. Caridina nilotica has been identified as a potential standard toxicity test organism, as it is widely distributed, easy to find and it occurs in flowing waters. It is an indigenous species which can be easily cultured and maintained in the laboratory and is also ecologically important. Both adults and juveniles have been used successfully in acute toxicity tests at the Institute for Water Research (Rhodes University) and the ability to rear the organisms under laboratory conditions has allowed the development of chronic toxicity tests using C. nilotica. Chronic early life stage tests include continuous exposure of the early life stages, which are presumed to be the most sensitive for aquatic organisms. This study reports on the embryonic development of C. nilotica at the culture temperature of 24⁰C. Morphological developmental stages were monitored and measured and 7 developmental stages were identified. Based on the measurements of the features that were identified, toxicity tests using the reference chemicals sodium chloride (NaCl), sodium sulphate (Na₂SO₄) and cadmium chloride (CdCl₂) were undertaken to test the suitability of C. nilotica embryonic development for chronic toxicity tests for use in water resource management. The length, width, length:width ratios and area of the features decreased in size when exposed to the chemicals. The Lowest Observed Effect Concentration (LOEC) values were 2000mg/L for Na₂SO₄, 3000mg/L for NaCl and 0.31mg/L for CdCl₂. The No Observed Effect Concentration (NOEC) values were 1000mg/L for Na₂SO₄, 2000mg/L for NaCl and <0.31mg/L for CdCl₂. Further research on the teratogenic effects of single chemicals and industrial effluent on developing C. nilotica embryos needs to be undertaken in order to evaluate the described test protocol for use in water resource management.
- Full Text:
- Authors: Ketse, Noziphiwo
- Date: 2007
- Subjects: Toxicity testing -- South Africa , Shrimp fisheries -- South Africa , Decapoda , Caridina , Water quality management -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6025 , http://hdl.handle.net/10962/d1005367 , Toxicity testing -- South Africa , Shrimp fisheries -- South Africa , Decapoda , Caridina , Water quality management -- South Africa
- Description: Aquatic toxicity tests are increasingly being used in water resource management worldwide, and currently in South Africa, policy and legislation are being drafted to reflect this international trend. While standard toxicity test methods and test organisms are being considered to develop and set water quality guidelines and effluent discharge limits, it is not clear whether guidelines and discharge limits set using these standard test organisms will be sufficient to protect South Africa’s scarce water resources. As part of ongoing research to investigate the use of indigenous riverine organisms as toxicity test organisms a number of potential species have been identified, including the freshwater shrimp Caridina nilotica. For much of the history of aquatic toxicological data the bulk of the data has been generated by acute toxicity testing, based on short exposures and using mortality as the response end point. There are relatively few chronic, longterm tests with sub-lethal endpoints. However, it was recognized that information about longer exposure durations and non lethal response endpoints was needed, instead of mortality. Chronic tests can provide a more environmentally realistic measure of chemical toxicity than acute toxicity tests. Caridina nilotica has been identified as a potential standard toxicity test organism, as it is widely distributed, easy to find and it occurs in flowing waters. It is an indigenous species which can be easily cultured and maintained in the laboratory and is also ecologically important. Both adults and juveniles have been used successfully in acute toxicity tests at the Institute for Water Research (Rhodes University) and the ability to rear the organisms under laboratory conditions has allowed the development of chronic toxicity tests using C. nilotica. Chronic early life stage tests include continuous exposure of the early life stages, which are presumed to be the most sensitive for aquatic organisms. This study reports on the embryonic development of C. nilotica at the culture temperature of 24⁰C. Morphological developmental stages were monitored and measured and 7 developmental stages were identified. Based on the measurements of the features that were identified, toxicity tests using the reference chemicals sodium chloride (NaCl), sodium sulphate (Na₂SO₄) and cadmium chloride (CdCl₂) were undertaken to test the suitability of C. nilotica embryonic development for chronic toxicity tests for use in water resource management. The length, width, length:width ratios and area of the features decreased in size when exposed to the chemicals. The Lowest Observed Effect Concentration (LOEC) values were 2000mg/L for Na₂SO₄, 3000mg/L for NaCl and 0.31mg/L for CdCl₂. The No Observed Effect Concentration (NOEC) values were 1000mg/L for Na₂SO₄, 2000mg/L for NaCl and <0.31mg/L for CdCl₂. Further research on the teratogenic effects of single chemicals and industrial effluent on developing C. nilotica embryos needs to be undertaken in order to evaluate the described test protocol for use in water resource management.
- Full Text:
The impact of load and frequency on the biomechanical, physiological and perceptual responses to dynamic pushing
- Authors: Cripwell, Adam Michael
- Date: 2007
- Subjects: Work -- Physiological aspects , Psychophysiology , Human engineering , Biomechanics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5144 , http://hdl.handle.net/10962/d1008183 , Work -- Physiological aspects , Psychophysiology , Human engineering , Biomechanics
- Description: The objective of the present research was to establish the biomechanical, physiological and perceptual responses of male operators to dynamic pushing tasks. The pushing tasks were performed using an industrial pallet jack with varying load/frequency combinations, in a controlled laboratory environment. Thirty healthy male subjects comprised the sample. Experimental procedures were conducted utilising the Chatillon ™ Dynamometer to measure force output in the initial, sustained and ending phases. The K4b2 Ergospirometer was used to assess physiological responses (heart rate and oxygen consumption [V02])' Nine recorded forces and nine experimental conditions formed the basis of this study, with subjects required to push three loads (200kg, 350kg, 500kg) at three frequencies (1120 sec, 1/40 sec, 1/60 sec) at a speed of 3.6km.h-1 over 14 metres on a co-efficient of friction controlled walkway for six minutes. Gait analysis, along with perceptions of exertion (,Central ' and 'Local' RPE) were collected during the third and sixth minutes of each condition . Body discomfort and contribution were identified upon completion of each condition. The results demonstrated that load and frequency interacted to influence responses within each domain. Increasing loads required increased force output during each stage of the push, which had a concomitant effect on physiological and perceptual responses. Significant differences arose between the initial, sustained and ending forces for each load, showing the direct relationship between load and force exertion. The combination of heaviest load/quickest frequency required the greatest physiological output, exceeding recommended guidelines for heart rate, V02 and energy expenditure responses. Intermediate combinations required moderate and acceptable energy cost. Linear relationships were established between heart rate and oxygen consumption , as well as between load and V02 , thus providing industrial practitioners an opportunity to evaluate task demands in situ. The combination of high forces and elevated physiological responses increased the subjective rating of the condition. The results emphasise the need to holistically consider all contributing factors in a dynamic pushing task. Dynamic pushing tasks place biomechanical, physiological and perceptual demands on the human operator, which must be minimised in order to ensure that this form of manual materials handling becomes sustainable in the long term.
- Full Text:
- Authors: Cripwell, Adam Michael
- Date: 2007
- Subjects: Work -- Physiological aspects , Psychophysiology , Human engineering , Biomechanics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5144 , http://hdl.handle.net/10962/d1008183 , Work -- Physiological aspects , Psychophysiology , Human engineering , Biomechanics
- Description: The objective of the present research was to establish the biomechanical, physiological and perceptual responses of male operators to dynamic pushing tasks. The pushing tasks were performed using an industrial pallet jack with varying load/frequency combinations, in a controlled laboratory environment. Thirty healthy male subjects comprised the sample. Experimental procedures were conducted utilising the Chatillon ™ Dynamometer to measure force output in the initial, sustained and ending phases. The K4b2 Ergospirometer was used to assess physiological responses (heart rate and oxygen consumption [V02])' Nine recorded forces and nine experimental conditions formed the basis of this study, with subjects required to push three loads (200kg, 350kg, 500kg) at three frequencies (1120 sec, 1/40 sec, 1/60 sec) at a speed of 3.6km.h-1 over 14 metres on a co-efficient of friction controlled walkway for six minutes. Gait analysis, along with perceptions of exertion (,Central ' and 'Local' RPE) were collected during the third and sixth minutes of each condition . Body discomfort and contribution were identified upon completion of each condition. The results demonstrated that load and frequency interacted to influence responses within each domain. Increasing loads required increased force output during each stage of the push, which had a concomitant effect on physiological and perceptual responses. Significant differences arose between the initial, sustained and ending forces for each load, showing the direct relationship between load and force exertion. The combination of heaviest load/quickest frequency required the greatest physiological output, exceeding recommended guidelines for heart rate, V02 and energy expenditure responses. Intermediate combinations required moderate and acceptable energy cost. Linear relationships were established between heart rate and oxygen consumption , as well as between load and V02 , thus providing industrial practitioners an opportunity to evaluate task demands in situ. The combination of high forces and elevated physiological responses increased the subjective rating of the condition. The results emphasise the need to holistically consider all contributing factors in a dynamic pushing task. Dynamic pushing tasks place biomechanical, physiological and perceptual demands on the human operator, which must be minimised in order to ensure that this form of manual materials handling becomes sustainable in the long term.
- Full Text:
The mutual embodiment of landscape and livelihoods: an environmental history of Nqabara
- Authors: De Klerk, Henning
- Date: 2007
- Subjects: Land reform -- South Africa -- Eastern Cape , Human ecology -- South Africa -- Eastern Cape , Human beings -- Effect of environment on -- South Africa -- Eastern Cape , Land use -- Environmental aspects -- South Africa -- Eastern Cape , Landscapes -- Social aspects -- South Africa -- Eastern Cape , Landscape ecology -- South Africa -- Eastern Cape , Land use, Rural -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4750 , http://hdl.handle.net/10962/d1007054 , Land reform -- South Africa -- Eastern Cape , Human ecology -- South Africa -- Eastern Cape , Human beings -- Effect of environment on -- South Africa -- Eastern Cape , Land use -- Environmental aspects -- South Africa -- Eastern Cape , Landscapes -- Social aspects -- South Africa -- Eastern Cape , Landscape ecology -- South Africa -- Eastern Cape , Land use, Rural -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape
- Description: This thesis presents a history of the landscape of Nqabara, an administrative area in a rural and coastal area of the Eastern Cape Province, South Africa. In the process of investigating landscape history, the inquiry engages with a range of data sources from diverging discursive contexts, including data from ethnographic fieldwork, from the consultation of archival documents and historical reports as well as from comparative historic and ethnographic research, necessitating a critical consideration of the epistemological contexts of data production and the dialogue between researcher and data. Furthermore, in its aim to move beyond historical description towards explanation, the study interrogates the dualist ontological conceptualisations of nature and culture, society and ecology, object and meaning upon which are built three dominant conceptual frameworks concerned with human-environment relationships: social-ecological systems theory, transdisciplinary landscape studies and political ecology. Drawing primarily upon the works of James Gibson, Anthony Giddens and Tim Ingold, an ontological foundation is developed to guide the enquiry and move towards an alternative understanding of the relationship of people’s livelihoods with respect to the landscape in which it is lived, which I call here the praxisembodiment perspective. This ontology takes the situated patterns of action of a situated agent-in-its-environment as its point of departure and proceeds to develop a framework explaining how relations among the patterns of action of different agents-in-their-environment, emerge in structures that simultaneously enable and constrain future action. The foundation is thereby provided for a monist understanding of how landscape and social structure emerge simultaneously from the complex intersection of patterns of actions and interactions of agents in their environment. This framework calls for an understanding of time, space and scale, not as independent variables influencing process and action, but as emergent properties arising from the patterns of actions of situated agents. Finally the alternative ontology is applied to the history of landscape and livelihoods of people of Nqabara. It is concluded that an appropriate understanding and explanation of the critical transformations in the landscape as well as in social institutions, should be sought through analysis of the complex ways in which patterns of action of multiple spatial and temporal rhythms and between multiple agents in an environment, intersect and resonate.
- Full Text:
- Authors: De Klerk, Henning
- Date: 2007
- Subjects: Land reform -- South Africa -- Eastern Cape , Human ecology -- South Africa -- Eastern Cape , Human beings -- Effect of environment on -- South Africa -- Eastern Cape , Land use -- Environmental aspects -- South Africa -- Eastern Cape , Landscapes -- Social aspects -- South Africa -- Eastern Cape , Landscape ecology -- South Africa -- Eastern Cape , Land use, Rural -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4750 , http://hdl.handle.net/10962/d1007054 , Land reform -- South Africa -- Eastern Cape , Human ecology -- South Africa -- Eastern Cape , Human beings -- Effect of environment on -- South Africa -- Eastern Cape , Land use -- Environmental aspects -- South Africa -- Eastern Cape , Landscapes -- Social aspects -- South Africa -- Eastern Cape , Landscape ecology -- South Africa -- Eastern Cape , Land use, Rural -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape
- Description: This thesis presents a history of the landscape of Nqabara, an administrative area in a rural and coastal area of the Eastern Cape Province, South Africa. In the process of investigating landscape history, the inquiry engages with a range of data sources from diverging discursive contexts, including data from ethnographic fieldwork, from the consultation of archival documents and historical reports as well as from comparative historic and ethnographic research, necessitating a critical consideration of the epistemological contexts of data production and the dialogue between researcher and data. Furthermore, in its aim to move beyond historical description towards explanation, the study interrogates the dualist ontological conceptualisations of nature and culture, society and ecology, object and meaning upon which are built three dominant conceptual frameworks concerned with human-environment relationships: social-ecological systems theory, transdisciplinary landscape studies and political ecology. Drawing primarily upon the works of James Gibson, Anthony Giddens and Tim Ingold, an ontological foundation is developed to guide the enquiry and move towards an alternative understanding of the relationship of people’s livelihoods with respect to the landscape in which it is lived, which I call here the praxisembodiment perspective. This ontology takes the situated patterns of action of a situated agent-in-its-environment as its point of departure and proceeds to develop a framework explaining how relations among the patterns of action of different agents-in-their-environment, emerge in structures that simultaneously enable and constrain future action. The foundation is thereby provided for a monist understanding of how landscape and social structure emerge simultaneously from the complex intersection of patterns of actions and interactions of agents in their environment. This framework calls for an understanding of time, space and scale, not as independent variables influencing process and action, but as emergent properties arising from the patterns of actions of situated agents. Finally the alternative ontology is applied to the history of landscape and livelihoods of people of Nqabara. It is concluded that an appropriate understanding and explanation of the critical transformations in the landscape as well as in social institutions, should be sought through analysis of the complex ways in which patterns of action of multiple spatial and temporal rhythms and between multiple agents in an environment, intersect and resonate.
- Full Text:
The role of aardvarks (Orycteropus afer) as ecosystem engineers in arid and semi-arid landscapes of South Africa
- Whittington-Jones, Gareth Morgan
- Authors: Whittington-Jones, Gareth Morgan
- Date: 2007
- Subjects: Aardvark , Aardvark -- South Africa , Aardvark -- South Africa -- Habitations , Animal burrowing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5757 , http://hdl.handle.net/10962/d1005445 , Aardvark , Aardvark -- South Africa , Aardvark -- South Africa -- Habitations , Animal burrowing
- Description: Arid and semi arid environments are characterised by extreme fluctuations in temperature and low rainfall which present significant challenges to the animals inhabiting these areas. Mammals, such as aardvarks (Orycteropus afer, Pallas 1766), excavate burrows in order to avoid predators and climatic extremes and are termed “ecosystem engineers” as they physically modify their environment and in doing so create new habitats and alter the availability of resources to other species. In this study I assessed the microhabitat conditions (maximum and minimum temperature, relative humidity and seed abundance) of aardvark burrows in relation to paired control sites. In addition, I evaluated the use of aardvark burrows by other vertebrate and invertebrate species and investigated the impact of aardvark burrow mounds on landscape scale floristic diversity. Maximum temperatures were significantly lower (p < 0.05) and minimum temperatures and midday humidity were significantly higher (p < 0.05) inside the burrows at the three study sites, Kwandwe Private Game Reserve (Kwandwe), Mountain Zebra National Park (MZNP) and Tswalu Kalahari Reserve (Tswalu). There were no significant differences between the concentration of seeds, the average numbers of unique individual small mammals, trap success or small mammal species richness recorded inside the burrows compared to outside (p > 0.05). At all three sites, small mammal species diversity was higher in the burrows but this result was also not significant (p > 0.05 for all). Trap success and the number of individuals captured was higher at Tswalu than the other two sites (p < 0.05 for both). The different methods used in this study revealed a total of 25 mammal, seven bird, one amphibian and six reptile species utilising aardvark burrows. There were significant differences in insect community assemblages between the burrows and open control areas at Kwandwe and Tswalu (p < 0.05 for both) but not at MZNP (p > 0.05). The parasitic guild was more prominent inside the burrows than outside but their abundance was not as high as anticipated, possibly due to the placement of traps closer to the burrow entrances than the sleeping chambers. The complex structure of the burrows prevented the placement of traps in close proximity to the sleeping chambers. As expected, the amount of bare earth was significantly higher on active and recently abandoned burrow mounds compared to the old burrow mounds and reference plots at all three sites (p < 0.05 for all), with the exception of the active burrows at Tswalu. Overall, the different plot types were characterised by significantly different plant communities during all the seasons at MZNP, during three of the seasons at Kwandwe and only during winter at Tswalu. The total species richness recorded on the reference plots was higher than on the burrow mounds at all three sites. However, species diversity on the reference plots was not significantly higher than the burrows at any of the sites (p > 0.05 for all sites). Although the results were not significant, the overall species diversity at a site level was greater than the reference patches at Kwandwe and Tswalu (p > 0.05 for both). Aardvarks fulfil the criteria of a significant ecosystem engineer and their presence in arid and semi-arid environments is likely to be critical to the survival of other individual organisms and species, particularly when alternative burrowing animals are either absent or restricted in their activities. Thus, aardvark populations should be considered a conservation priority in arid and semi-arid ecosystems.
- Full Text:
- Authors: Whittington-Jones, Gareth Morgan
- Date: 2007
- Subjects: Aardvark , Aardvark -- South Africa , Aardvark -- South Africa -- Habitations , Animal burrowing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5757 , http://hdl.handle.net/10962/d1005445 , Aardvark , Aardvark -- South Africa , Aardvark -- South Africa -- Habitations , Animal burrowing
- Description: Arid and semi arid environments are characterised by extreme fluctuations in temperature and low rainfall which present significant challenges to the animals inhabiting these areas. Mammals, such as aardvarks (Orycteropus afer, Pallas 1766), excavate burrows in order to avoid predators and climatic extremes and are termed “ecosystem engineers” as they physically modify their environment and in doing so create new habitats and alter the availability of resources to other species. In this study I assessed the microhabitat conditions (maximum and minimum temperature, relative humidity and seed abundance) of aardvark burrows in relation to paired control sites. In addition, I evaluated the use of aardvark burrows by other vertebrate and invertebrate species and investigated the impact of aardvark burrow mounds on landscape scale floristic diversity. Maximum temperatures were significantly lower (p < 0.05) and minimum temperatures and midday humidity were significantly higher (p < 0.05) inside the burrows at the three study sites, Kwandwe Private Game Reserve (Kwandwe), Mountain Zebra National Park (MZNP) and Tswalu Kalahari Reserve (Tswalu). There were no significant differences between the concentration of seeds, the average numbers of unique individual small mammals, trap success or small mammal species richness recorded inside the burrows compared to outside (p > 0.05). At all three sites, small mammal species diversity was higher in the burrows but this result was also not significant (p > 0.05 for all). Trap success and the number of individuals captured was higher at Tswalu than the other two sites (p < 0.05 for both). The different methods used in this study revealed a total of 25 mammal, seven bird, one amphibian and six reptile species utilising aardvark burrows. There were significant differences in insect community assemblages between the burrows and open control areas at Kwandwe and Tswalu (p < 0.05 for both) but not at MZNP (p > 0.05). The parasitic guild was more prominent inside the burrows than outside but their abundance was not as high as anticipated, possibly due to the placement of traps closer to the burrow entrances than the sleeping chambers. The complex structure of the burrows prevented the placement of traps in close proximity to the sleeping chambers. As expected, the amount of bare earth was significantly higher on active and recently abandoned burrow mounds compared to the old burrow mounds and reference plots at all three sites (p < 0.05 for all), with the exception of the active burrows at Tswalu. Overall, the different plot types were characterised by significantly different plant communities during all the seasons at MZNP, during three of the seasons at Kwandwe and only during winter at Tswalu. The total species richness recorded on the reference plots was higher than on the burrow mounds at all three sites. However, species diversity on the reference plots was not significantly higher than the burrows at any of the sites (p > 0.05 for all sites). Although the results were not significant, the overall species diversity at a site level was greater than the reference patches at Kwandwe and Tswalu (p > 0.05 for both). Aardvarks fulfil the criteria of a significant ecosystem engineer and their presence in arid and semi-arid environments is likely to be critical to the survival of other individual organisms and species, particularly when alternative burrowing animals are either absent or restricted in their activities. Thus, aardvark populations should be considered a conservation priority in arid and semi-arid ecosystems.
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The role of arbuscular mycorrhizal fungi in the biotransformation of coal and application in dump rehabilitation
- Mukasa-Mugerwa, Thomas Tendo
- Authors: Mukasa-Mugerwa, Thomas Tendo
- Date: 2007
- Subjects: Vesicular-arbuscular mycorrhizas , Mycorrhizal fungi , Fungi -- Biotechnology , Bermuda grass , Coal mines and mining -- Environmental aspects , Acid mine drainage
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3999 , http://hdl.handle.net/10962/d1004059 , Vesicular-arbuscular mycorrhizas , Mycorrhizal fungi , Fungi -- Biotechnology , Bermuda grass , Coal mines and mining -- Environmental aspects , Acid mine drainage
- Description: Fundamental processes underpinning the biotransformation of coal by fungal biocatalysts have been intensively investigated, however, limited large-scale industrial applications using such systems have been reported. The un-anticipated sporadic growth of Cynodon dactylon on the surface of un-rehabilitated discard coal dumps has been noted and this was found to be coupled with the breakdown of coal into a humic soil-like material in the top 1.5 metres of the dumps. Extensive fungal growth was observed to be associated with the Cynodon dactylon root system and examination of plant roots indicated the presence of mycorrhizal fungi. Analysis of the Cynodon dactylon plant roots around which coal biotransformation was occurring confirmed the presence of arbuscular mycorrhizal colonisation with the species Glomus clarum, Paraglomus occultum, Gigaspora gigantea and Glomus mosseae identified to be associated with the plants. Further molecular characterisation of non-mycorrhizal rhizospheric fungi showed the presence of fungal species with coal-degrading capabilities that most likely played a role in the coal biotransformation observed. The discard coal dump environment was simulated in pot and column studies and coal biotransformation was reproduced, with this process enhanced by the addition of mycorrhizal and non-mycorrhizal rhizospheric fungal inocula to the environment. Mycorrhizal and non-mycorrhizal species in the inoculum were re-isolated from the simulated environment fulfilling a number of Koch’s postulates and indicating a causal role in the biotransformation of coal. An inversion of conventional mycorrhizal colonisation was demonstrated in this system with reduction in extraradicular presence and an increase in intracellular colonisation compared to soil controls. A descriptive model was formulated suggesting a two-part fungal system involving organic carbon and nutrient exchange between the plant, mycorrhizal fungi and non-mycorrhizal coal-degrading rhizospheric fungi ultimately resulting in the biotransformation of coal. The biotransformation observed was comparable to reports of “rock-eating fungi”. Results suggest that the biological degradation of coal in situ with the production of a soil-like substrate could provide a feasible method of discard coal dump rehabilitation as well as provide a humic-rich substrate that can be utilised in further industrial applications.
- Full Text:
- Authors: Mukasa-Mugerwa, Thomas Tendo
- Date: 2007
- Subjects: Vesicular-arbuscular mycorrhizas , Mycorrhizal fungi , Fungi -- Biotechnology , Bermuda grass , Coal mines and mining -- Environmental aspects , Acid mine drainage
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3999 , http://hdl.handle.net/10962/d1004059 , Vesicular-arbuscular mycorrhizas , Mycorrhizal fungi , Fungi -- Biotechnology , Bermuda grass , Coal mines and mining -- Environmental aspects , Acid mine drainage
- Description: Fundamental processes underpinning the biotransformation of coal by fungal biocatalysts have been intensively investigated, however, limited large-scale industrial applications using such systems have been reported. The un-anticipated sporadic growth of Cynodon dactylon on the surface of un-rehabilitated discard coal dumps has been noted and this was found to be coupled with the breakdown of coal into a humic soil-like material in the top 1.5 metres of the dumps. Extensive fungal growth was observed to be associated with the Cynodon dactylon root system and examination of plant roots indicated the presence of mycorrhizal fungi. Analysis of the Cynodon dactylon plant roots around which coal biotransformation was occurring confirmed the presence of arbuscular mycorrhizal colonisation with the species Glomus clarum, Paraglomus occultum, Gigaspora gigantea and Glomus mosseae identified to be associated with the plants. Further molecular characterisation of non-mycorrhizal rhizospheric fungi showed the presence of fungal species with coal-degrading capabilities that most likely played a role in the coal biotransformation observed. The discard coal dump environment was simulated in pot and column studies and coal biotransformation was reproduced, with this process enhanced by the addition of mycorrhizal and non-mycorrhizal rhizospheric fungal inocula to the environment. Mycorrhizal and non-mycorrhizal species in the inoculum were re-isolated from the simulated environment fulfilling a number of Koch’s postulates and indicating a causal role in the biotransformation of coal. An inversion of conventional mycorrhizal colonisation was demonstrated in this system with reduction in extraradicular presence and an increase in intracellular colonisation compared to soil controls. A descriptive model was formulated suggesting a two-part fungal system involving organic carbon and nutrient exchange between the plant, mycorrhizal fungi and non-mycorrhizal coal-degrading rhizospheric fungi ultimately resulting in the biotransformation of coal. The biotransformation observed was comparable to reports of “rock-eating fungi”. Results suggest that the biological degradation of coal in situ with the production of a soil-like substrate could provide a feasible method of discard coal dump rehabilitation as well as provide a humic-rich substrate that can be utilised in further industrial applications.
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