Implementing non-photorealistic rendering enhancements with real-time performance
- Authors: Winnemöller, Holger
- Date: 2002 , 2013-05-09
- Subjects: Computer animation , Computer graphics , Real-time data processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4580 , http://hdl.handle.net/10962/d1003135 , Computer animation , Computer graphics , Real-time data processing
- Description: We describe quality and performance enhancements, which work in real-time, to all well-known Non-photorealistic (NPR) rendering styles for use in an interactive context. These include Comic rendering, Sketch rendering, Hatching and Painterly rendering, but we also attempt and justify a widening of the established definition of what is considered NPR. In the individual Chapters, we identify typical stylistic elements of the different NPR styles. We list problems that need to be solved in order to implement the various renderers. Standard solutions available in the literature are introduced and in all cases extended and optimised. In particular, we extend the lighting model of the comic renderer to include a specular component and introduce multiple inter-related but independent geometric approximations which greatly improve rendering performance. We implement two completely different solutions to random perturbation sketching, solve temporal coherence issues for coal sketching and find an unexpected use for 3D textures to implement hatch-shading. Textured brushes of painterly rendering are extended by properties such as stroke-direction and texture, motion, paint capacity, opacity and emission, making them more flexible and versatile. Brushes are also provided with a minimal amount of intelligence, so that they can help in maximising screen coverage of brushes. We furthermore devise a completely new NPR style, which we call super-realistic and show how sample images can be tweened in real-time to produce an image-based six degree-of-freedom renderer performing at roughly 450 frames per second. Performance values for our other renderers all lie between 10 and over 400 frames per second on homePC hardware, justifying our real-time claim. A large number of sample screen-shots, illustrations and animations demonstrate the visual fidelity of our rendered images. In essence, we successfully achieve our attempted goals of increasing the creative, expressive and communicative potential of individual NPR styles, increasing performance of most of them, adding original and interesting visual qualities, and exploring new techniques or existing ones in novel ways. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2002
- Authors: Winnemöller, Holger
- Date: 2002 , 2013-05-09
- Subjects: Computer animation , Computer graphics , Real-time data processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4580 , http://hdl.handle.net/10962/d1003135 , Computer animation , Computer graphics , Real-time data processing
- Description: We describe quality and performance enhancements, which work in real-time, to all well-known Non-photorealistic (NPR) rendering styles for use in an interactive context. These include Comic rendering, Sketch rendering, Hatching and Painterly rendering, but we also attempt and justify a widening of the established definition of what is considered NPR. In the individual Chapters, we identify typical stylistic elements of the different NPR styles. We list problems that need to be solved in order to implement the various renderers. Standard solutions available in the literature are introduced and in all cases extended and optimised. In particular, we extend the lighting model of the comic renderer to include a specular component and introduce multiple inter-related but independent geometric approximations which greatly improve rendering performance. We implement two completely different solutions to random perturbation sketching, solve temporal coherence issues for coal sketching and find an unexpected use for 3D textures to implement hatch-shading. Textured brushes of painterly rendering are extended by properties such as stroke-direction and texture, motion, paint capacity, opacity and emission, making them more flexible and versatile. Brushes are also provided with a minimal amount of intelligence, so that they can help in maximising screen coverage of brushes. We furthermore devise a completely new NPR style, which we call super-realistic and show how sample images can be tweened in real-time to produce an image-based six degree-of-freedom renderer performing at roughly 450 frames per second. Performance values for our other renderers all lie between 10 and over 400 frames per second on homePC hardware, justifying our real-time claim. A large number of sample screen-shots, illustrations and animations demonstrate the visual fidelity of our rendered images. In essence, we successfully achieve our attempted goals of increasing the creative, expressive and communicative potential of individual NPR styles, increasing performance of most of them, adding original and interesting visual qualities, and exploring new techniques or existing ones in novel ways. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2002
Investigation of the bioconversion of constituents of olive effluents for the production of valuable chemical compounds
- Authors: Notshe, Thandiwe Loretta
- Date: 2002
- Subjects: Phenols , Sewage -- Purification , Effluent quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4078 , http://hdl.handle.net/10962/d1007446 , Phenols , Sewage -- Purification , Effluent quality
- Description: Olive mill wastewater is produced in large quantities during the production of olive oil and olive production effluents are produced during the processing of olives. This project was planned to find a use for constituents found in olive production wastewater. The task was carried out by first characterizing the olive effluents, then screening microorganisms for growth in the effluents and reduction of the pollutant properties of the effluents. An investigation into the biotransformation of aromatic compounds present in the effluents into useful chemicals, was carried out. The olive production effluents were collected from different stages in the process for treating olive wastewater, viz, a fermentation tank (FB), the surface of a digester (LV) and an evaporation pond (SO). The three effluents were characterized by investigating their phenolic composition. Protocatechuic acid, vanillic acid, syringic acid, hydroxyphenyl acetic acid, coumaric acid and ferulic acid were identified in an olive effluent, FB, using thin layer chromatography (TLC) and High perfomance liquid chromatography (HPLC). Hydroxyphenyl acetic acid constitutes almost 60% of the organics in olive effluent FB. Five bacteria, namely RU-LV1; RU-FBI and RU-FB2; RU-SOI and RU-S02, were isolated from the olive effluents LV, FB and SO respectively. These isolates were found to be halotolerant and were able to grow over a broad temperature and pH range, with the maximum temperature and pH for growth being 28°C and pH 7 respectively. A range of microorganisms were evaluated for their ability to grow and reduce the total phenolic content of the olive effluents. Among these Neurospora crassa showed the highest potential for the biological reduction of total phenolics in olive effluents. Approximately 70% of the total phenolic content was removed by N. crassa. Trametes verscilor, Pseudomonas putida strains, RU-KMI and RU-KM3s, and the bacteria isolated from olive effluents could also degrade the total phenolic content of olive effluents, but to a lesser extent. The ability of the five bacterial isolates to grow and degrade aromatic compounds was assessed by growing them in medium with standard aromatic compounds. RU-L V1 degraded 96%, 100%, 73% and 100% of caffeic acid, protocatechuic acid, p-coumaric acid and vanillic acid respectively. The other isolates degraded caffeic acid and protocatechuic acid, but their ability to degraded p-coumaric acid and vanillic acid was found to be lesser than the ability of RU-LV1 to degrade the same aromatic compounds. Whole cells of RU-LV1 degraded vanillic acid but no metabolic products were observed on HPLC analysis. Resting cells, French pressed extract, cell free extracts and cell debris from RU-LV1 cells induced with vanillic acid degraded vanillic acid, ferulic acid and vanillin at rates higher than those obtained from non-induced cultures. No products were observed during the degradation of vanillic acid. Ferulic acid was converted into vanillic acid by French pressed extract, cell free extract and cell debris of RU-LV1. The maximum yield of vanillic acid as a product (0 .23 mM, 50 %yield) was obtained when cell free extracts of RU-LVI, grown in glucose and induced by vanillic acid, were used for the degradation of 0.4 mM ferulic acid. Vanillin was rapidly converted into vanillic acid by resting cells, cell free extracts and French pressed extract of RU-LVI. Using molecular techniques, the similarity ranking of the RU-LVI 16S rRNA gene and its clone showed a high similarity to Corynebacterium glutamicum and Corynebacterium acedopltilum. The rapid degradation of vanillin to vanillic acid suggests that extracts from RU-LV1 degrade ferulic acid into vanillin which is immediately oxidized to vanillic acid. Vanillic acid is also considered as a high value chemical. This project has a potential of producing useful chemicals from cheap substrates that can be found in olive effluents. , KMBT_363
- Full Text:
- Date Issued: 2002
- Authors: Notshe, Thandiwe Loretta
- Date: 2002
- Subjects: Phenols , Sewage -- Purification , Effluent quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4078 , http://hdl.handle.net/10962/d1007446 , Phenols , Sewage -- Purification , Effluent quality
- Description: Olive mill wastewater is produced in large quantities during the production of olive oil and olive production effluents are produced during the processing of olives. This project was planned to find a use for constituents found in olive production wastewater. The task was carried out by first characterizing the olive effluents, then screening microorganisms for growth in the effluents and reduction of the pollutant properties of the effluents. An investigation into the biotransformation of aromatic compounds present in the effluents into useful chemicals, was carried out. The olive production effluents were collected from different stages in the process for treating olive wastewater, viz, a fermentation tank (FB), the surface of a digester (LV) and an evaporation pond (SO). The three effluents were characterized by investigating their phenolic composition. Protocatechuic acid, vanillic acid, syringic acid, hydroxyphenyl acetic acid, coumaric acid and ferulic acid were identified in an olive effluent, FB, using thin layer chromatography (TLC) and High perfomance liquid chromatography (HPLC). Hydroxyphenyl acetic acid constitutes almost 60% of the organics in olive effluent FB. Five bacteria, namely RU-LV1; RU-FBI and RU-FB2; RU-SOI and RU-S02, were isolated from the olive effluents LV, FB and SO respectively. These isolates were found to be halotolerant and were able to grow over a broad temperature and pH range, with the maximum temperature and pH for growth being 28°C and pH 7 respectively. A range of microorganisms were evaluated for their ability to grow and reduce the total phenolic content of the olive effluents. Among these Neurospora crassa showed the highest potential for the biological reduction of total phenolics in olive effluents. Approximately 70% of the total phenolic content was removed by N. crassa. Trametes verscilor, Pseudomonas putida strains, RU-KMI and RU-KM3s, and the bacteria isolated from olive effluents could also degrade the total phenolic content of olive effluents, but to a lesser extent. The ability of the five bacterial isolates to grow and degrade aromatic compounds was assessed by growing them in medium with standard aromatic compounds. RU-L V1 degraded 96%, 100%, 73% and 100% of caffeic acid, protocatechuic acid, p-coumaric acid and vanillic acid respectively. The other isolates degraded caffeic acid and protocatechuic acid, but their ability to degraded p-coumaric acid and vanillic acid was found to be lesser than the ability of RU-LV1 to degrade the same aromatic compounds. Whole cells of RU-LV1 degraded vanillic acid but no metabolic products were observed on HPLC analysis. Resting cells, French pressed extract, cell free extracts and cell debris from RU-LV1 cells induced with vanillic acid degraded vanillic acid, ferulic acid and vanillin at rates higher than those obtained from non-induced cultures. No products were observed during the degradation of vanillic acid. Ferulic acid was converted into vanillic acid by French pressed extract, cell free extract and cell debris of RU-LV1. The maximum yield of vanillic acid as a product (0 .23 mM, 50 %yield) was obtained when cell free extracts of RU-LVI, grown in glucose and induced by vanillic acid, were used for the degradation of 0.4 mM ferulic acid. Vanillin was rapidly converted into vanillic acid by resting cells, cell free extracts and French pressed extract of RU-LVI. Using molecular techniques, the similarity ranking of the RU-LVI 16S rRNA gene and its clone showed a high similarity to Corynebacterium glutamicum and Corynebacterium acedopltilum. The rapid degradation of vanillin to vanillic acid suggests that extracts from RU-LV1 degrade ferulic acid into vanillin which is immediately oxidized to vanillic acid. Vanillic acid is also considered as a high value chemical. This project has a potential of producing useful chemicals from cheap substrates that can be found in olive effluents. , KMBT_363
- Full Text:
- Date Issued: 2002
Modelling the distribution and abundance of several demersal fish species on the Agulhas Bank, South Africa
- Authors: Sampson, Mark Robert
- Date: 2002
- Subjects: Fishes -- South Africa -- Geographical distribution , Fishes -- South Africa -- Geographical distribution -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5343 , http://hdl.handle.net/10962/d1006207 , Fishes -- South Africa -- Geographical distribution , Fishes -- South Africa -- Geographical distribution -- Mathematical models
- Description: The Agulhas Bank supports a speciose fish community, many of which are commercially important. Despite substantial research being conducted on aspects of their biology spatial aspects of their distribution and abundance in relation to environment parameters has been ignored. This study, therefore, addressed aspects related to the distribution and abundance of representative species on the Agulhas Bank within a Geographic Information System (GIS). Four candidate species were chosen due to their importance either in numbers or unit mass to the South African demersal trawl fishery. The species also shared morphological and taxonomic similarities. The candidate species chosen were the two Cape hake species, shallow-water hake Meluccius capensis, and deep-water hake Merluccius paradoxus, and the two pleuronectiform species being Agulhas sole Austroglossus pectoralis and redspotted tonguesole Cynoglossus zanzibarensis. The use of a GIS was appropriate and allowed for hidden spatial patterns be exposed and illustrated visually, while also facilitating the quantification of the relationships between distribution/abundance and certain environmental predictors using statistical methods The Department of Marine and Coastal Management, Cape Town, supplied biological data in the form of length frequency and biomass information from spring (AprillMay) and autumn (September/October) cruises conducted between 1986 and 1993 on the R. V. Africana. The Council for National Geoscience, Cape Town, supplied sediment data for the entire southern African coastline. Initial exploratory data analysis highlighted potential relationships between environmental variables and abundance for each specie's life-history stanzas. Variations in spatial distribution were found to be significantly different between each life-history stanzas within species. Fish density as a function of the additive effects of the various environmental parameters, including temperature, depth and sediment type, was assessed using a Poisson Generalized Additive Model (GAM), while distribution was analysed with a logistic GAM. A predictive logistic model was then created, taking into consideration the importance of the predictor variables for each species, allowing for predictive estimates to be made for each species by inputting environmental information within the study area. The importance of certain environmental variables influencing distribution and abundance were noted. General patterns indicated that sediment was the most important to both the distribution and abundance of the two pleuronectiform species and juvenile life-history stanzas, while the adult gadoids' distribution and abundance appeared to be depth dependent.
- Full Text:
- Date Issued: 2002
- Authors: Sampson, Mark Robert
- Date: 2002
- Subjects: Fishes -- South Africa -- Geographical distribution , Fishes -- South Africa -- Geographical distribution -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5343 , http://hdl.handle.net/10962/d1006207 , Fishes -- South Africa -- Geographical distribution , Fishes -- South Africa -- Geographical distribution -- Mathematical models
- Description: The Agulhas Bank supports a speciose fish community, many of which are commercially important. Despite substantial research being conducted on aspects of their biology spatial aspects of their distribution and abundance in relation to environment parameters has been ignored. This study, therefore, addressed aspects related to the distribution and abundance of representative species on the Agulhas Bank within a Geographic Information System (GIS). Four candidate species were chosen due to their importance either in numbers or unit mass to the South African demersal trawl fishery. The species also shared morphological and taxonomic similarities. The candidate species chosen were the two Cape hake species, shallow-water hake Meluccius capensis, and deep-water hake Merluccius paradoxus, and the two pleuronectiform species being Agulhas sole Austroglossus pectoralis and redspotted tonguesole Cynoglossus zanzibarensis. The use of a GIS was appropriate and allowed for hidden spatial patterns be exposed and illustrated visually, while also facilitating the quantification of the relationships between distribution/abundance and certain environmental predictors using statistical methods The Department of Marine and Coastal Management, Cape Town, supplied biological data in the form of length frequency and biomass information from spring (AprillMay) and autumn (September/October) cruises conducted between 1986 and 1993 on the R. V. Africana. The Council for National Geoscience, Cape Town, supplied sediment data for the entire southern African coastline. Initial exploratory data analysis highlighted potential relationships between environmental variables and abundance for each specie's life-history stanzas. Variations in spatial distribution were found to be significantly different between each life-history stanzas within species. Fish density as a function of the additive effects of the various environmental parameters, including temperature, depth and sediment type, was assessed using a Poisson Generalized Additive Model (GAM), while distribution was analysed with a logistic GAM. A predictive logistic model was then created, taking into consideration the importance of the predictor variables for each species, allowing for predictive estimates to be made for each species by inputting environmental information within the study area. The importance of certain environmental variables influencing distribution and abundance were noted. General patterns indicated that sediment was the most important to both the distribution and abundance of the two pleuronectiform species and juvenile life-history stanzas, while the adult gadoids' distribution and abundance appeared to be depth dependent.
- Full Text:
- Date Issued: 2002
Physiological and perceptual responses of SANDF personnel to varying combinations of marching speed and backpack load
- Authors: Christie, Candice Jo-Anne
- Date: 2002
- Subjects: Marching -- Physiological aspects , Walking -- Physiological aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5128 , http://hdl.handle.net/10962/d1005207 , Marching -- Physiological aspects , Walking -- Physiological aspects
- Description: The objective of the present study was to establish effective combinations of marching speed and backpack load in order to meet specific military requirements. Thirty infantrymen from the South African National Defence Force (SANDF) comprised the sample and experimental procedures were conducted in a laboratory setting using a Cybex Trotter treadmill. Sixteen conditions were set up which included combinations of four speeds (3.5, 4.5, 5.5, and 6.5 km.h⁻¹) and four backpack loads (20, 35, 50, and 65kg). Each subject was required to complete 8 of the sixteen conditions, each consisting of a six-minute treadmill march. Physiological data (heart rate, ventilation and metabolic responses), kinematic gait responses (step-rate and stride length) and perceptions of exertion (“Central” and “Local” RPE) were collected during the third and sixth minutes of the treadmill march and areas of body discomfort were identified post-march. Responses revealed five distinct categories of exertional strain. Three marches constituted “nominal” (below 40% VO₂max) and three “excessive” strain (above 75% VO₂ max). These represent combinations of extreme military demands and are highly unlikely to be utilised by the military. Three “tolerable” levels of required effort were recommended and these 10 combinations were further divided into three sub-categories. The “moderate” stress marches were identified as “ideal” for prolonged marches and had statistically similar responses of working heart rates (range of 118 bt.min⁻¹ to 127 bt.min⁻¹), energy expenditure (26 kJ.min⁻¹ and 27 kJ.min⁻¹) and ratings of perceived exertion (“Central” ratings of 10 and 11). Thus, marching at 5.5 km.h⁻¹with 20kg, 4.5 km.h⁻¹ with 35kg or 3.5 km.h⁻¹ with 50kg all require a similar energy cost. Four “heavy” category marches were identified for possible use when the duration of the march is reduced. During these marches responses were statistically similar with heart rates ranging from 127 bt.min⁻¹ to 137 bt.min⁻¹, energy expenditure from 32 kJ.min⁻¹ to 37 kJ.min⁻¹ and “Central” ratings of perceived exertion were 12 and 13. When short, high intensity marches are necessary, then combinations from the “very heavy” category may be utilised but with caution. During these marches, soldiers were taxed between 65% and 75% of VO2 max. The results of this study clearly demonstrate that the interplay between speed and load needs to be adjusted when determining “ideal” combinations for specific military demands. Essentially, if speed is of the essence then load must be reduced, and if heavy loads need to be transported then speed must be reduced.
- Full Text:
- Date Issued: 2002
- Authors: Christie, Candice Jo-Anne
- Date: 2002
- Subjects: Marching -- Physiological aspects , Walking -- Physiological aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5128 , http://hdl.handle.net/10962/d1005207 , Marching -- Physiological aspects , Walking -- Physiological aspects
- Description: The objective of the present study was to establish effective combinations of marching speed and backpack load in order to meet specific military requirements. Thirty infantrymen from the South African National Defence Force (SANDF) comprised the sample and experimental procedures were conducted in a laboratory setting using a Cybex Trotter treadmill. Sixteen conditions were set up which included combinations of four speeds (3.5, 4.5, 5.5, and 6.5 km.h⁻¹) and four backpack loads (20, 35, 50, and 65kg). Each subject was required to complete 8 of the sixteen conditions, each consisting of a six-minute treadmill march. Physiological data (heart rate, ventilation and metabolic responses), kinematic gait responses (step-rate and stride length) and perceptions of exertion (“Central” and “Local” RPE) were collected during the third and sixth minutes of the treadmill march and areas of body discomfort were identified post-march. Responses revealed five distinct categories of exertional strain. Three marches constituted “nominal” (below 40% VO₂max) and three “excessive” strain (above 75% VO₂ max). These represent combinations of extreme military demands and are highly unlikely to be utilised by the military. Three “tolerable” levels of required effort were recommended and these 10 combinations were further divided into three sub-categories. The “moderate” stress marches were identified as “ideal” for prolonged marches and had statistically similar responses of working heart rates (range of 118 bt.min⁻¹ to 127 bt.min⁻¹), energy expenditure (26 kJ.min⁻¹ and 27 kJ.min⁻¹) and ratings of perceived exertion (“Central” ratings of 10 and 11). Thus, marching at 5.5 km.h⁻¹with 20kg, 4.5 km.h⁻¹ with 35kg or 3.5 km.h⁻¹ with 50kg all require a similar energy cost. Four “heavy” category marches were identified for possible use when the duration of the march is reduced. During these marches responses were statistically similar with heart rates ranging from 127 bt.min⁻¹ to 137 bt.min⁻¹, energy expenditure from 32 kJ.min⁻¹ to 37 kJ.min⁻¹ and “Central” ratings of perceived exertion were 12 and 13. When short, high intensity marches are necessary, then combinations from the “very heavy” category may be utilised but with caution. During these marches, soldiers were taxed between 65% and 75% of VO2 max. The results of this study clearly demonstrate that the interplay between speed and load needs to be adjusted when determining “ideal” combinations for specific military demands. Essentially, if speed is of the essence then load must be reduced, and if heavy loads need to be transported then speed must be reduced.
- Full Text:
- Date Issued: 2002
Physiological and psychophysical responses of male soldiers to changes in marching gradient, speed and load
- Authors: Todd, Andrew Ivan
- Date: 2002
- Subjects: Soldiers -- South Africa -- Physiology , South Africa Army -- Physical training , Marching -- Physiological aspects , Psychophysiology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5129 , http://hdl.handle.net/10962/d1005208 , Soldiers -- South Africa -- Physiology , South Africa Army -- Physical training , Marching -- Physiological aspects , Psychophysiology
- Description: The present study sought to investigate the effects of changes in gradient, under apparently optimal combinations of speed and load, on selected physiological, psychophysical and biophysical responses of military personnel. Subjects (n = 32) were required to march under level (0%), downhill (-10%) and uphill (+10%) conditions. Under each gradient, subjects marched with the following speed-load combinations: 4 km.h-1 carrying 50 kg, 5 km.h-1 carrying 35 kg and 6 km.h-1 carrying 20 kg, a total of nine experimental conditions. Subjects were required to march for six minutes under each condition. Physiological responses (HR, VO2, R, Br, VE, VT, EE) indicated that subjects were not overly taxed by the three speed-load combinations during level marching, which elicited submaximal demands. Furthermore, the results revealed that downhill marching with heavy loads results in similar responses to level marching, while lighter loads may result in significant reductions in physical demands compared to level marching. The physiological responses to uphill marching revealed that subjects were severely physically taxed under these conditions, regardless of speed-load combination. It is unlikely that soldiers would be able to maintain these intensities for an extended period without undue fatigue. It is evident from the psychophysical responses (Rating of Perceived Exertion and Body Discomfort) that subjects perceived the heavy load conditions, regardless of gradient, to be the most stressful on the cardiovascular and muscular systems. The positive gradient conditions also elicited elevated RPE and Body Discomfort responses, while lighter load downhill conditions were perceived to result in the least strain.
- Full Text:
- Date Issued: 2002
- Authors: Todd, Andrew Ivan
- Date: 2002
- Subjects: Soldiers -- South Africa -- Physiology , South Africa Army -- Physical training , Marching -- Physiological aspects , Psychophysiology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5129 , http://hdl.handle.net/10962/d1005208 , Soldiers -- South Africa -- Physiology , South Africa Army -- Physical training , Marching -- Physiological aspects , Psychophysiology
- Description: The present study sought to investigate the effects of changes in gradient, under apparently optimal combinations of speed and load, on selected physiological, psychophysical and biophysical responses of military personnel. Subjects (n = 32) were required to march under level (0%), downhill (-10%) and uphill (+10%) conditions. Under each gradient, subjects marched with the following speed-load combinations: 4 km.h-1 carrying 50 kg, 5 km.h-1 carrying 35 kg and 6 km.h-1 carrying 20 kg, a total of nine experimental conditions. Subjects were required to march for six minutes under each condition. Physiological responses (HR, VO2, R, Br, VE, VT, EE) indicated that subjects were not overly taxed by the three speed-load combinations during level marching, which elicited submaximal demands. Furthermore, the results revealed that downhill marching with heavy loads results in similar responses to level marching, while lighter loads may result in significant reductions in physical demands compared to level marching. The physiological responses to uphill marching revealed that subjects were severely physically taxed under these conditions, regardless of speed-load combination. It is unlikely that soldiers would be able to maintain these intensities for an extended period without undue fatigue. It is evident from the psychophysical responses (Rating of Perceived Exertion and Body Discomfort) that subjects perceived the heavy load conditions, regardless of gradient, to be the most stressful on the cardiovascular and muscular systems. The positive gradient conditions also elicited elevated RPE and Body Discomfort responses, while lighter load downhill conditions were perceived to result in the least strain.
- Full Text:
- Date Issued: 2002
Speed-related isokinetic and psychophysical responses of female military personnel
- Authors: Kennedy, Dale Charles
- Date: 2002
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:21028 , http://hdl.handle.net/10962/6063
- Description: The present study aims to contribute in an area that has long been neglected, the indigenous female population, about whose physical strength and work-capacity very little is known. Speed-related isokinetic and psychophysical responses of female military personnel were assessed (N=32). Furthermore benchmark data was established for Work-Simulation isokinetic responses where there appears to be limited publication. Analysis of the data showed significant differences in torque, work and power outputs as velocity increased. Only wrench-turning left and right rotation for peak torque and total work did not show significant reductions. Consistency of effort level recorded an average of 72% across the selected isokinetic tests. No discernible morphological and strength expression differences were observed between military office workers and infantry personnel, and no significant correlations were recorded between heart rate and RPE values.
- Full Text:
- Date Issued: 2002
- Authors: Kennedy, Dale Charles
- Date: 2002
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:21028 , http://hdl.handle.net/10962/6063
- Description: The present study aims to contribute in an area that has long been neglected, the indigenous female population, about whose physical strength and work-capacity very little is known. Speed-related isokinetic and psychophysical responses of female military personnel were assessed (N=32). Furthermore benchmark data was established for Work-Simulation isokinetic responses where there appears to be limited publication. Analysis of the data showed significant differences in torque, work and power outputs as velocity increased. Only wrench-turning left and right rotation for peak torque and total work did not show significant reductions. Consistency of effort level recorded an average of 72% across the selected isokinetic tests. No discernible morphological and strength expression differences were observed between military office workers and infantry personnel, and no significant correlations were recorded between heart rate and RPE values.
- Full Text:
- Date Issued: 2002
Synthetic approaches to marine labdane diterpenes
- Authors: Wisch, Gregory Albert
- Date: 2002 , 2013-05-10
- Subjects: Diterpenes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4289 , http://hdl.handle.net/10962/d1003131 , Diterpenes
- Description: The work presented in this thesis describes the synthesis of labd-13-en-8ß, 15-diol (46) a stable reduced derivative of an unstable marine natural product aldehyde [8ß-hydroxylabd-13E-en-15-al (6)] isolated by Cimino, et al. from the skin of a Notaspidean mollusc Pleurobranchaea meckelii. The rationale for the synthesis was to provide sufficient 46 for eventual mild oxidation to 6 and investigation of the biological activity of this latter compound. (-)-Sclareol (32), a common diterpene synthetic precursor, was the starting point for the ten step synthesis of 46 described in this thesis. A search of the literature revealed that only one non stereospecific synthesis of 46 had been previously reported. To provide the necessary background to the synthetic component of this thesis, both the occurrence of labdane and ent-labdane in the marine environment and the use of sclareol in the synthesis of marine natural products, was reviewed. The initial step in the synthesis of 46 was the potassium permanganate oxidation of sclareol to give a bisnorlabdane ketone. Reduction of this ketone with lithium aluminium hydride produced a mixture of diols which, through t-butyldimethysilyl triflate protection, afforded a quantitative route to protecting the C-13 ketone in an effort to prevent unwanted intramolecular cyclization reactions. Dehydration of the tertiary alcohol moiety at C-8 with phosphorous oxychloride yielded the Δ⁸·¹⁵ exocyclic alkene required for the next oxidation step. Ozonolysis succeeded in producing the desired C-8 ketone after much deliberation and research into alternative oxidation strategies. Methyl lithium methylation quantitatively afforded the desired a-methyl substitution at C-8, identified at the onset as the key step in the synthesis. Tetra-butylarnmonium fluoride deprotection yielded the 8ß,13-dihydoxylated product, which was in turn subjected to a Swern oxidition to give the desired 8ß-hydroxy-bisnorlabda-13-one. A modified Horner-Wadsworth-Emmons reaction allowed for elaboration at C-13 to yield a to Δ¹³ olefin with a terminal C-15 ethyl ester. Diisobutylaluminium hydride reduction of the ester produced the desired labd-13-en-8ß, 15-diol (46) in a low overall yield of 3.4%. Although opportunities for optimization of at least three steps in the synthesis exist, time constraints prevented both optimization of these steps and an investigation of the oxidation of 46 to 6. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2002
- Authors: Wisch, Gregory Albert
- Date: 2002 , 2013-05-10
- Subjects: Diterpenes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4289 , http://hdl.handle.net/10962/d1003131 , Diterpenes
- Description: The work presented in this thesis describes the synthesis of labd-13-en-8ß, 15-diol (46) a stable reduced derivative of an unstable marine natural product aldehyde [8ß-hydroxylabd-13E-en-15-al (6)] isolated by Cimino, et al. from the skin of a Notaspidean mollusc Pleurobranchaea meckelii. The rationale for the synthesis was to provide sufficient 46 for eventual mild oxidation to 6 and investigation of the biological activity of this latter compound. (-)-Sclareol (32), a common diterpene synthetic precursor, was the starting point for the ten step synthesis of 46 described in this thesis. A search of the literature revealed that only one non stereospecific synthesis of 46 had been previously reported. To provide the necessary background to the synthetic component of this thesis, both the occurrence of labdane and ent-labdane in the marine environment and the use of sclareol in the synthesis of marine natural products, was reviewed. The initial step in the synthesis of 46 was the potassium permanganate oxidation of sclareol to give a bisnorlabdane ketone. Reduction of this ketone with lithium aluminium hydride produced a mixture of diols which, through t-butyldimethysilyl triflate protection, afforded a quantitative route to protecting the C-13 ketone in an effort to prevent unwanted intramolecular cyclization reactions. Dehydration of the tertiary alcohol moiety at C-8 with phosphorous oxychloride yielded the Δ⁸·¹⁵ exocyclic alkene required for the next oxidation step. Ozonolysis succeeded in producing the desired C-8 ketone after much deliberation and research into alternative oxidation strategies. Methyl lithium methylation quantitatively afforded the desired a-methyl substitution at C-8, identified at the onset as the key step in the synthesis. Tetra-butylarnmonium fluoride deprotection yielded the 8ß,13-dihydoxylated product, which was in turn subjected to a Swern oxidition to give the desired 8ß-hydroxy-bisnorlabda-13-one. A modified Horner-Wadsworth-Emmons reaction allowed for elaboration at C-13 to yield a to Δ¹³ olefin with a terminal C-15 ethyl ester. Diisobutylaluminium hydride reduction of the ester produced the desired labd-13-en-8ß, 15-diol (46) in a low overall yield of 3.4%. Although opportunities for optimization of at least three steps in the synthesis exist, time constraints prevented both optimization of these steps and an investigation of the oxidation of 46 to 6. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2002
The biology and molecular ecology of floating sulphur biofilms
- Authors: Bowker, Michelle Louise
- Date: 2002
- Subjects: Biofilms , Microbial ecology , Sulfur
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4056 , http://hdl.handle.net/10962/d1004117 , Biofilms , Microbial ecology , Sulfur
- Description: Floating sulphur biofilms have been observed to occur on sulphate-containing natural systems and waste stabilization ponds. It has been postulated that these biofilms form on the surface of the water because sulphate reducing bacteria present in the bottom layers of the water body reduce sulphate to sulphide which then diffuses upwards and is oxidized under the correct redox conditions to sulphur by sulphide oxidizing bacteria. Very little information exists on these complex floating systems and in order to study them further, model systems were designed. The Baffle Reactor was successfully used to cultivate floating sulphur biofilms. Conditions within the reactor could be closely scrutinized in the laboratory and it was found that sulphate levels decreased, sulphide levels increased and that sulphur was produced over a period of 2 weeks. The success of this system led to it being scaled-up and currently a method to harvest sulphur from the biofilm is under development. It is thought that biofilms are highly complex, heterogeneous structures with different bacteria distributed in different layers. Preliminary work suggested that bacteria were differentially distributed along nutrient and oxygen gradients within the biofilm. Biofilms are very thin structures and therefore difficult to study and Gradient systems were developed in an attempt to spatially separate the biofilm species into functional layers. Gradient Tubes were designed; these provided a gradient of high-sulphide, low oxygen conditions to high-oxygen, low-sulphide conditions. Bacteria were observed to grow in different layers of these systems. The Gradient Tubes could be sectioned and the chemical characteristics of each section as well as the species present could be determined. Silicon Tubular Bioreactors were also developed and these were very efficient at producing large amounts of sulphur under strictly controlled redox conditions. Microscopy and molecular methods including the amplification of a section of Ribosomal Ribonucleic acid by Polymerase Chain Reaction were used in an attempt to characterize the populations present in these biofilm systems. Denaturing Gradient Gel Electrophoresis was used to create band profiles of the populations; individual bands were excised from the gels and sequenced. Identified species included Ectothiorhodospira sp., Dethiosulfovibrio russensis, Pseudomonas geniculata, Thiobacillus baregensis and Halothiobacillus kellyi.
- Full Text:
- Date Issued: 2002
- Authors: Bowker, Michelle Louise
- Date: 2002
- Subjects: Biofilms , Microbial ecology , Sulfur
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4056 , http://hdl.handle.net/10962/d1004117 , Biofilms , Microbial ecology , Sulfur
- Description: Floating sulphur biofilms have been observed to occur on sulphate-containing natural systems and waste stabilization ponds. It has been postulated that these biofilms form on the surface of the water because sulphate reducing bacteria present in the bottom layers of the water body reduce sulphate to sulphide which then diffuses upwards and is oxidized under the correct redox conditions to sulphur by sulphide oxidizing bacteria. Very little information exists on these complex floating systems and in order to study them further, model systems were designed. The Baffle Reactor was successfully used to cultivate floating sulphur biofilms. Conditions within the reactor could be closely scrutinized in the laboratory and it was found that sulphate levels decreased, sulphide levels increased and that sulphur was produced over a period of 2 weeks. The success of this system led to it being scaled-up and currently a method to harvest sulphur from the biofilm is under development. It is thought that biofilms are highly complex, heterogeneous structures with different bacteria distributed in different layers. Preliminary work suggested that bacteria were differentially distributed along nutrient and oxygen gradients within the biofilm. Biofilms are very thin structures and therefore difficult to study and Gradient systems were developed in an attempt to spatially separate the biofilm species into functional layers. Gradient Tubes were designed; these provided a gradient of high-sulphide, low oxygen conditions to high-oxygen, low-sulphide conditions. Bacteria were observed to grow in different layers of these systems. The Gradient Tubes could be sectioned and the chemical characteristics of each section as well as the species present could be determined. Silicon Tubular Bioreactors were also developed and these were very efficient at producing large amounts of sulphur under strictly controlled redox conditions. Microscopy and molecular methods including the amplification of a section of Ribosomal Ribonucleic acid by Polymerase Chain Reaction were used in an attempt to characterize the populations present in these biofilm systems. Denaturing Gradient Gel Electrophoresis was used to create band profiles of the populations; individual bands were excised from the gels and sequenced. Identified species included Ectothiorhodospira sp., Dethiosulfovibrio russensis, Pseudomonas geniculata, Thiobacillus baregensis and Halothiobacillus kellyi.
- Full Text:
- Date Issued: 2002
The characterisation of a South African isolate of Cryptophlebia leucotreta Granulovirus (CIGV)
- Authors: Singh, Shalene
- Date: 2002
- Subjects: Cryptophlebia leucotreta , Cryptophlebia leucotreta -- Control , Pests -- Biological control -- Africa , DNA viruses
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4067 , http://hdl.handle.net/10962/d1004929 , Cryptophlebia leucotreta , Cryptophlebia leucotreta -- Control , Pests -- Biological control -- Africa , DNA viruses
- Description: The false codling moth (FCM), Cryptophlehia Leucatreta, causes widespread damage to economically important fruit crops throughout sub-Saharan Africa. Fruit are rendered unfit for consumption once they have been stung by FCM larvae. Larval infestation of fruit can lead to significant pre-harvest losses or post-harvest waste, posing a major problem to the citrus industry. Current control of the pest includes the use of chemical pesticides. The larval form of FCM is known to be infected by a granulovirus called Cryptophlebia leucotreta granulovirus (CIGV). Granuloviruses are highly specific against their hosts and are harmless to vertebrates, plants and the environment. The development of CIGV into a biological control agent would offer an attractive and safer alternative for the control of this pest. A full characterisation of CIGV is required prior to the virus being disseminated into the environment. In this project, the characteristics of CIGV will be examined. Viral DNA was extracted from infected larvae and the DNA analysed by restriction fragment length polymorphism (RFLP). Fragmentation profiles of the South African and Cape Verde (CV3) isolates of the virus were compared, revealing distinct differences between them. The size of the CIGV-SA genome was calculated to be 112 kbp, identical to the size of the CV3 isolate. Physical maps for five restriction enzymes were constructed for the CIGV-SA genome. The alignment of these maps with maps the CV3 isolate (for the same enzymes) further highlighted the differences between the isolates. The genetic engineering of granuloviruses could significantly improve the speed of kill of these viruses. Therefore essential genes like egt and granulin were isolated (by PCR) and their position located in the genome. Both genes were sequenced and their phylogeny with other granulin and egt genes investigated. Finally, tbe incidence of CIGV in natural populations of FCM larvae was investigated, by screening field-collected larvae for the presence of the virus. CIGV was successfully detected from dot blots of larval DNA using both radiolabelled and non-radiolabelled probes and by PCR. Trends regarding the incidence of CIGV in natural populations of larvae were also determined.
- Full Text:
- Date Issued: 2002
- Authors: Singh, Shalene
- Date: 2002
- Subjects: Cryptophlebia leucotreta , Cryptophlebia leucotreta -- Control , Pests -- Biological control -- Africa , DNA viruses
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4067 , http://hdl.handle.net/10962/d1004929 , Cryptophlebia leucotreta , Cryptophlebia leucotreta -- Control , Pests -- Biological control -- Africa , DNA viruses
- Description: The false codling moth (FCM), Cryptophlehia Leucatreta, causes widespread damage to economically important fruit crops throughout sub-Saharan Africa. Fruit are rendered unfit for consumption once they have been stung by FCM larvae. Larval infestation of fruit can lead to significant pre-harvest losses or post-harvest waste, posing a major problem to the citrus industry. Current control of the pest includes the use of chemical pesticides. The larval form of FCM is known to be infected by a granulovirus called Cryptophlebia leucotreta granulovirus (CIGV). Granuloviruses are highly specific against their hosts and are harmless to vertebrates, plants and the environment. The development of CIGV into a biological control agent would offer an attractive and safer alternative for the control of this pest. A full characterisation of CIGV is required prior to the virus being disseminated into the environment. In this project, the characteristics of CIGV will be examined. Viral DNA was extracted from infected larvae and the DNA analysed by restriction fragment length polymorphism (RFLP). Fragmentation profiles of the South African and Cape Verde (CV3) isolates of the virus were compared, revealing distinct differences between them. The size of the CIGV-SA genome was calculated to be 112 kbp, identical to the size of the CV3 isolate. Physical maps for five restriction enzymes were constructed for the CIGV-SA genome. The alignment of these maps with maps the CV3 isolate (for the same enzymes) further highlighted the differences between the isolates. The genetic engineering of granuloviruses could significantly improve the speed of kill of these viruses. Therefore essential genes like egt and granulin were isolated (by PCR) and their position located in the genome. Both genes were sequenced and their phylogeny with other granulin and egt genes investigated. Finally, tbe incidence of CIGV in natural populations of FCM larvae was investigated, by screening field-collected larvae for the presence of the virus. CIGV was successfully detected from dot blots of larval DNA using both radiolabelled and non-radiolabelled probes and by PCR. Trends regarding the incidence of CIGV in natural populations of larvae were also determined.
- Full Text:
- Date Issued: 2002
The effect of energy and protein nutritional levels on production of breeding ostriches
- Authors: Brand, Zanell
- Date: 2002
- Subjects: Ostriches -- Nutrition , Ostriches -- Breeding--South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5805 , http://hdl.handle.net/10962/d1006312 , Ostriches -- Nutrition , Ostriches -- Breeding--South Africa
- Description: Adequate and appropriate nutrition is essential for the production of high quality commercially farmed animal species. Although South Africa has had a well-established ostrich industry for over a century, little information on ostrich nutrition, in particular the specific nutritional requirements at different stages of production, is available. The industry has consequently relied heavily on data derived from poultry and pigs, but this has often proved to be unsatisfactory for ostriches and has resulted in serious nutritional-related problems. Recent studies on the metabolisable energy of specific components of diet formulations and balanced diets have indicated that ostriches have enhanced digestibility compared with poultry and pigs. At the same time, in the present economic climate, it is necessary to find cost-effective diets for breeding birds without compromising egg and chick production. This study primarily assessed the effect of different dietary protein and energy levels on production parameters and body condition of breeding female and male ostriches. The energy content of the diet appears to be the main constraint to egg production and breeders on low energy diets laid fewer eggs and lost more body condition compared with breeders fed higher energy diets. A diet with 8.5 MJ/kg DM and 10% protein with well balanced amino acids appears to be sufficient for female breeders without compromising production and a diet with 7.5 MJ/kg DM and 10% protein appears suitable for breeding male ostriches. Different dietary energy and protein levels similarly had little or no effect on egg quality and composition. This study also assessed the carry-over effect of the nutritional regime in one year on the production in the following breeding season. Females fed diets as low as 7.5 MJ/kg ME in the previous breeding season produced significantly fewer eggs in the next breeding season, which resulted in lower chick production. Energy had no effect on the percentage infertile eggs or on the initial egg weight. Different levels of dietary protein had no effect on egg production, egg weight, fertility, hatchability and initial chick weight.
- Full Text:
- Date Issued: 2002
- Authors: Brand, Zanell
- Date: 2002
- Subjects: Ostriches -- Nutrition , Ostriches -- Breeding--South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5805 , http://hdl.handle.net/10962/d1006312 , Ostriches -- Nutrition , Ostriches -- Breeding--South Africa
- Description: Adequate and appropriate nutrition is essential for the production of high quality commercially farmed animal species. Although South Africa has had a well-established ostrich industry for over a century, little information on ostrich nutrition, in particular the specific nutritional requirements at different stages of production, is available. The industry has consequently relied heavily on data derived from poultry and pigs, but this has often proved to be unsatisfactory for ostriches and has resulted in serious nutritional-related problems. Recent studies on the metabolisable energy of specific components of diet formulations and balanced diets have indicated that ostriches have enhanced digestibility compared with poultry and pigs. At the same time, in the present economic climate, it is necessary to find cost-effective diets for breeding birds without compromising egg and chick production. This study primarily assessed the effect of different dietary protein and energy levels on production parameters and body condition of breeding female and male ostriches. The energy content of the diet appears to be the main constraint to egg production and breeders on low energy diets laid fewer eggs and lost more body condition compared with breeders fed higher energy diets. A diet with 8.5 MJ/kg DM and 10% protein with well balanced amino acids appears to be sufficient for female breeders without compromising production and a diet with 7.5 MJ/kg DM and 10% protein appears suitable for breeding male ostriches. Different dietary energy and protein levels similarly had little or no effect on egg quality and composition. This study also assessed the carry-over effect of the nutritional regime in one year on the production in the following breeding season. Females fed diets as low as 7.5 MJ/kg ME in the previous breeding season produced significantly fewer eggs in the next breeding season, which resulted in lower chick production. Energy had no effect on the percentage infertile eggs or on the initial egg weight. Different levels of dietary protein had no effect on egg production, egg weight, fertility, hatchability and initial chick weight.
- Full Text:
- Date Issued: 2002
The effect of nutrient levels and ratios on the growth of Microcystis aeruginosa and microcystin production
- Authors: Sember, Craig Stewart
- Date: 2002
- Subjects: Microcystis aeruginosa -- Toxicology , Nitrates , Microcystins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11076 , http://hdl.handle.net/10948/287 , Microcystis aeruginosa -- Toxicology , Nitrates , Microcystins
- Description: This study reports the findings on the effect of nitrates and phosphates on the biomass and toxin production of various strains of the unicellular non-nitrogen fixing cyanobacterium, Microcystis aeruginosa. The occurrence of blooms of Microcystis aeruginosa and microcystin in freshwater impoundments across the globe has been on the increase lately due to increased levels of eutrophication, resulting in human and animal deaths and illness, as well as drinking and recreational water foulment. A range of environmental factors have been shown to effect growth and microcystin production. Existing literature however is somewhat contradictory as to the effects of these physical and chemical factors on toxin production. Therefore Microcystis aeruginosa strains were cultured under batch and continuous conditions to determine the effect of nitrate and phosphate concentrations and ratios on biomass and toxin production. Cultures were analysed with regards to internal nutrient stores, biomass production, nutrient depletion, photosynthetic efficiency and microcystin production. Results showed that microcystin production correlated to growth rate, photosynthetic efficiency and internal nitrogen stores and that an optimal N:P ratio was associated with microcystin levels, growth rate and photosynthetic efficiency. Results therefore led to the conclusion that the nitrogen, carbon, and phosphate balance within the cell is closely associated with microcystin production. Whether or not microcystin is produced to maintain this balance or produced as a function of this balance remains to be determined.
- Full Text:
- Date Issued: 2002
- Authors: Sember, Craig Stewart
- Date: 2002
- Subjects: Microcystis aeruginosa -- Toxicology , Nitrates , Microcystins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11076 , http://hdl.handle.net/10948/287 , Microcystis aeruginosa -- Toxicology , Nitrates , Microcystins
- Description: This study reports the findings on the effect of nitrates and phosphates on the biomass and toxin production of various strains of the unicellular non-nitrogen fixing cyanobacterium, Microcystis aeruginosa. The occurrence of blooms of Microcystis aeruginosa and microcystin in freshwater impoundments across the globe has been on the increase lately due to increased levels of eutrophication, resulting in human and animal deaths and illness, as well as drinking and recreational water foulment. A range of environmental factors have been shown to effect growth and microcystin production. Existing literature however is somewhat contradictory as to the effects of these physical and chemical factors on toxin production. Therefore Microcystis aeruginosa strains were cultured under batch and continuous conditions to determine the effect of nitrate and phosphate concentrations and ratios on biomass and toxin production. Cultures were analysed with regards to internal nutrient stores, biomass production, nutrient depletion, photosynthetic efficiency and microcystin production. Results showed that microcystin production correlated to growth rate, photosynthetic efficiency and internal nitrogen stores and that an optimal N:P ratio was associated with microcystin levels, growth rate and photosynthetic efficiency. Results therefore led to the conclusion that the nitrogen, carbon, and phosphate balance within the cell is closely associated with microcystin production. Whether or not microcystin is produced to maintain this balance or produced as a function of this balance remains to be determined.
- Full Text:
- Date Issued: 2002
The effect of selenium in the detoxification of the microcystin hepatotoxins
- Authors: Downs, Kerry
- Date: 2002
- Subjects: Cynaobacterial toxins , Microcystins , Selenium
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11070 , http://hdl.handle.net/10948/284 , Cynaobacterial toxins , Microcystins , Selenium
- Description: Blooms of cyanobacteria have been known to cause illness in humans and death in wild and domestic animals. One of the toxins produced by cyanobacteria is microcystin, which is a potent hepatotoxin. Microcystin is taken up by bile acid transporters in the intestine and transported into the liver. After exposure to acute doses of microcystin, severe haemorrhage has been observed along with apoptotic and necrotic hepatocytes. The cytoskeletal structure of the hepatocytes is disrupted and oxidative stress is induced. Selenium, a known anti-oxidant, has been shown to induce increased activity of glutathione peroxidase. Glutathione peroxidase removes peroxides from cells protecting them from oxidative stress. This study set out to determine if selenium could play a role in preventing the damage to mice livers due to microcystin toxin. The protective role of selenium was explored in three main studies: in the first study, the ability of selenium to increase the survival time of mice exposed to a lethal dose of toxin was determined. In the second study the mice were exposed to sublethal chronic doses of toxin over 30 days. The ability of selenium to minimise liver damage under these conditions was determined. The final study investigated the mechanism of the protective effect of selenium. The results of the first study suggested that selenium could extend survival time. In the second study the selenium supplemented mice showed a reduction in the extent of the increase in liver weight and a decrease in the amount of lipid peroxidation induced compared to the mice that received only toxin. The histology of the selenium supplemented mice also showed a decrease in the severity and amount of morphological changes in the liver. The third study indicated that the protection shown by selenium might be mediated by an increase in the glutathione peroxidase (GPX) activity in selenium supplemented mice. This increase in GPX activity would increase the removal of the lipid hydroperoxides and prevent the damage they would cause in the cell. A further result indicated an increase in glutathione S-transferase in only the toxin control mice when compared to the selenium supplemented and control mice. ii In conclusion selenium offers protection against microcystin but further studies need to be done to provide statistically valid results to clarify the level of protection.
- Full Text:
- Date Issued: 2002
- Authors: Downs, Kerry
- Date: 2002
- Subjects: Cynaobacterial toxins , Microcystins , Selenium
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11070 , http://hdl.handle.net/10948/284 , Cynaobacterial toxins , Microcystins , Selenium
- Description: Blooms of cyanobacteria have been known to cause illness in humans and death in wild and domestic animals. One of the toxins produced by cyanobacteria is microcystin, which is a potent hepatotoxin. Microcystin is taken up by bile acid transporters in the intestine and transported into the liver. After exposure to acute doses of microcystin, severe haemorrhage has been observed along with apoptotic and necrotic hepatocytes. The cytoskeletal structure of the hepatocytes is disrupted and oxidative stress is induced. Selenium, a known anti-oxidant, has been shown to induce increased activity of glutathione peroxidase. Glutathione peroxidase removes peroxides from cells protecting them from oxidative stress. This study set out to determine if selenium could play a role in preventing the damage to mice livers due to microcystin toxin. The protective role of selenium was explored in three main studies: in the first study, the ability of selenium to increase the survival time of mice exposed to a lethal dose of toxin was determined. In the second study the mice were exposed to sublethal chronic doses of toxin over 30 days. The ability of selenium to minimise liver damage under these conditions was determined. The final study investigated the mechanism of the protective effect of selenium. The results of the first study suggested that selenium could extend survival time. In the second study the selenium supplemented mice showed a reduction in the extent of the increase in liver weight and a decrease in the amount of lipid peroxidation induced compared to the mice that received only toxin. The histology of the selenium supplemented mice also showed a decrease in the severity and amount of morphological changes in the liver. The third study indicated that the protection shown by selenium might be mediated by an increase in the glutathione peroxidase (GPX) activity in selenium supplemented mice. This increase in GPX activity would increase the removal of the lipid hydroperoxides and prevent the damage they would cause in the cell. A further result indicated an increase in glutathione S-transferase in only the toxin control mice when compared to the selenium supplemented and control mice. ii In conclusion selenium offers protection against microcystin but further studies need to be done to provide statistically valid results to clarify the level of protection.
- Full Text:
- Date Issued: 2002
The effects of control design and working posture on strength and work output: an isokinetic investigation
- Dirkse Van Schalkwyk, Charles Joseph
- Authors: Dirkse Van Schalkwyk, Charles Joseph
- Date: 2002
- Subjects: Posture , Human engineering
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5118 , http://hdl.handle.net/10962/d1005196 , Posture , Human engineering
- Description: he objective of the present study was to assess the isokinetic, cardiovascular and psychophysical responses of young adult males (N=30) during valve turning exercises. It aimed to evaluate the variables in relation to changes in control design and working posture. Isokinetic testing and ergonomics have not been widely linked and it was an aim of this study to show the advantages to the field of ergonomics. Furthermore, the “work-simulation” package used in the present study has not been widely exploited and it was believed that this study could thus contribute significantly to the literature. Testing was carried out using a CYBEX ® 6000 isokinetic dynamometer, a polar heart watch, an Omron M1 semi-automatic blood pressure monitor and various perceptual rating scales. Testing involved the subjects having to perform 4 maximal turning efforts in 18 different conditions. These conditions were made up by using 6 different control designs in 3 varying positions. Subjects were required to attend two sessions, each approximately one hour long, in which nine randomised conditions were tested in each session. During these sessions, isokinetic responses: peak torque (Nm), total work (J) and average power (W); cardiovascular responses: heart rate (bt.min[superscript -1]) and blood pressure (mmHg); and psychophysical responses: RPE and discomfort, were observed. The results of the tests showed that in general significant differences were encountered for isokinetic, cardiovascular and psychophysical responses in relation to changes in the control design. However, significant differences were far less evident, and in most cases non existent, in relation to changes in the spatial orientation of the control types. The essence being that operator position with respect to the control is not as crucial as the control design.
- Full Text:
- Date Issued: 2002
- Authors: Dirkse Van Schalkwyk, Charles Joseph
- Date: 2002
- Subjects: Posture , Human engineering
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5118 , http://hdl.handle.net/10962/d1005196 , Posture , Human engineering
- Description: he objective of the present study was to assess the isokinetic, cardiovascular and psychophysical responses of young adult males (N=30) during valve turning exercises. It aimed to evaluate the variables in relation to changes in control design and working posture. Isokinetic testing and ergonomics have not been widely linked and it was an aim of this study to show the advantages to the field of ergonomics. Furthermore, the “work-simulation” package used in the present study has not been widely exploited and it was believed that this study could thus contribute significantly to the literature. Testing was carried out using a CYBEX ® 6000 isokinetic dynamometer, a polar heart watch, an Omron M1 semi-automatic blood pressure monitor and various perceptual rating scales. Testing involved the subjects having to perform 4 maximal turning efforts in 18 different conditions. These conditions were made up by using 6 different control designs in 3 varying positions. Subjects were required to attend two sessions, each approximately one hour long, in which nine randomised conditions were tested in each session. During these sessions, isokinetic responses: peak torque (Nm), total work (J) and average power (W); cardiovascular responses: heart rate (bt.min[superscript -1]) and blood pressure (mmHg); and psychophysical responses: RPE and discomfort, were observed. The results of the tests showed that in general significant differences were encountered for isokinetic, cardiovascular and psychophysical responses in relation to changes in the control design. However, significant differences were far less evident, and in most cases non existent, in relation to changes in the spatial orientation of the control types. The essence being that operator position with respect to the control is not as crucial as the control design.
- Full Text:
- Date Issued: 2002
The immobilization of Microcystis aeruginosa PCC7806 on a membrane nutrient-gradostat bioreacator for the production of the secondary metobolites
- Authors: Strong, Peter James
- Date: 2002
- Subjects: Microcystis aeruginosa , Myrocystins , Bioreactors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11083 , http://hdl.handle.net/10948/283 , Microcystis aeruginosa , Myrocystins , Bioreactors
- Description: A module and an inoculation technique were developed that would allow for the efficient immobilization of Microcystis aeruginosa PCC7806 on a synthetic membrane. A variety of module types, membranes (ceramic, tubular polyethersulfone and externally skinless polyethersulfone capillary membrane), and methods of immobilization (adsorption, pressure filtration and a developed technique that involved drying a cell slurry on a membrane) were assessed. The morphological properties that affected the immobilization of Microcystis aeruginosa PCC7806, as well as the effects of immobilization upon cell morphology were assessed. Cells in the stationary growth phase, which had a well-developed extra-cellular polysaccharide layer and no gas vesicles, were optimal for immobilization. Microcystin production under immobilized conditions was assessed under different nitrate concentrations, light intensities, biofilm thickness and immobilization times. Additional work included assaying for Microcystin production of two airlift-grown cultures under a high light intensity and complete nutrient deprivation and the inoculation of a ceramic membrane. An immunological technique was used to elucidate where toxin production was greatest within a biofilm immobilized upon an externally skinless polyethersulfone capillary membrane. The externally skinless polyethersulfone capillary membrane was evaluated to assess homogeneity and the physical differences between membrane batches that led to the erratic, incomplete biofilm formation, as a biofilm of a constant thickness could not be immobilized. Microcystis aeruginosa PCC7806 was exposed to a variety of solvents in order to permeabilize the cyanobacteria, as that would have enabled a truly continuous extraction process for the metabolite. FDA hydrolysis had to be optimized in order to use it as an indicator of cell viability. In addition a single-step extraction of Microcystin was attempted using live bacteria. A capillary membrane module, containing the externally skinless polyethersulfone capillary membrane, inoculated using pressure filtration, was the most efficient combination to establish a biofilm. Cells that were no longer actively dividing and that lacked buoyancy displayed superior immobilization to cells that were actively dividing and buoyant. The immobilized cells did produce Microcystin but in much lower concentrations to cells grown in an airlift culture. Biofilms grown with a higher nitrate concentration, a lower biofilm thickness and a lower light intensity had a higher specific microcystin content, while biofilms with a higher nitrate concentration a lower light intensity and a longer growth period displayed the a greater toxin production per mm2 of membrane. Microcystin occurred at its highest concentration in cells just above the pore opening. The diffusion of nutrients occurred relatively quickly to the outside layers of the biofilm, with a true gradient being established laterally from these nutrient veins that were above the pores. Permeabilization of the cells proved unsuccessful, as cells that remained viable did not release the intracellular compound into the surrounding medium.
- Full Text:
- Date Issued: 2002
- Authors: Strong, Peter James
- Date: 2002
- Subjects: Microcystis aeruginosa , Myrocystins , Bioreactors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11083 , http://hdl.handle.net/10948/283 , Microcystis aeruginosa , Myrocystins , Bioreactors
- Description: A module and an inoculation technique were developed that would allow for the efficient immobilization of Microcystis aeruginosa PCC7806 on a synthetic membrane. A variety of module types, membranes (ceramic, tubular polyethersulfone and externally skinless polyethersulfone capillary membrane), and methods of immobilization (adsorption, pressure filtration and a developed technique that involved drying a cell slurry on a membrane) were assessed. The morphological properties that affected the immobilization of Microcystis aeruginosa PCC7806, as well as the effects of immobilization upon cell morphology were assessed. Cells in the stationary growth phase, which had a well-developed extra-cellular polysaccharide layer and no gas vesicles, were optimal for immobilization. Microcystin production under immobilized conditions was assessed under different nitrate concentrations, light intensities, biofilm thickness and immobilization times. Additional work included assaying for Microcystin production of two airlift-grown cultures under a high light intensity and complete nutrient deprivation and the inoculation of a ceramic membrane. An immunological technique was used to elucidate where toxin production was greatest within a biofilm immobilized upon an externally skinless polyethersulfone capillary membrane. The externally skinless polyethersulfone capillary membrane was evaluated to assess homogeneity and the physical differences between membrane batches that led to the erratic, incomplete biofilm formation, as a biofilm of a constant thickness could not be immobilized. Microcystis aeruginosa PCC7806 was exposed to a variety of solvents in order to permeabilize the cyanobacteria, as that would have enabled a truly continuous extraction process for the metabolite. FDA hydrolysis had to be optimized in order to use it as an indicator of cell viability. In addition a single-step extraction of Microcystin was attempted using live bacteria. A capillary membrane module, containing the externally skinless polyethersulfone capillary membrane, inoculated using pressure filtration, was the most efficient combination to establish a biofilm. Cells that were no longer actively dividing and that lacked buoyancy displayed superior immobilization to cells that were actively dividing and buoyant. The immobilized cells did produce Microcystin but in much lower concentrations to cells grown in an airlift culture. Biofilms grown with a higher nitrate concentration, a lower biofilm thickness and a lower light intensity had a higher specific microcystin content, while biofilms with a higher nitrate concentration a lower light intensity and a longer growth period displayed the a greater toxin production per mm2 of membrane. Microcystin occurred at its highest concentration in cells just above the pore opening. The diffusion of nutrients occurred relatively quickly to the outside layers of the biofilm, with a true gradient being established laterally from these nutrient veins that were above the pores. Permeabilization of the cells proved unsuccessful, as cells that remained viable did not release the intracellular compound into the surrounding medium.
- Full Text:
- Date Issued: 2002
The independent high rate algal pond as a unit operation in tertiary wastewater treatment
- Authors: Clark, Stewart James
- Date: 2002
- Subjects: Algae -- Biotechnology , Sewage -- Purification -- Biological treatment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4092 , http://hdl.handle.net/10962/d1007805
- Description: The development of the High Rate Algal Pond (HRAP) as an independent tertiary treatment unit operation for phosphate and nitrate removal is reported. A novel Integrated Algal Ponding System (lAPS) design is proposed for nutrient removal from the effluents of both a conventional domestic sewage treatment plant and from an Advanced Integrated Wastewater Ponding System (AIWPS). The viability of an independently operated HRAP has been identified and termed the Independent High Rate Algal Pond (l-HRAP). A 500 m² pilot 1- HRAP was operated in such a way as to facilitate the precipitation of calcium phosphate, known to be controlled by pH (greater than 9.4) and resulting in final phosphate levels of less than 1 mg.L⁻¹ as P0₄-P. The incorporation of the I-HRAP into a denitrification process was also investigated. Continuously fed column reactors, utilising algal biomass as a carbon source, showed that the heterotrophic bacterial community dominant in the anaerobic algal sludge were denitrifying the nitrate in the feed. It was demonstrated that as the cultures were stressed (using increased nitrate concentrations, anaerobiosis and light starvation) total polysaccharide (TPS) concentrations increased, with a notable increase 111 the exopolysaccharide (EPS) fraction. These experiments corroborated the hypothesis that harvested microalgal biomass can be manipulated to produce, and release, exopolymeric substances under stress conditions, and which may serve as carbon source for denitrification. In both batch flask studies and in laboratory-scale reactor systems, harvested microalgal biomass from an HRAP was shown to produce exopolymeric substances under stress conditions. Initial high loading-rates of greater than 20 mg.L⁻¹ NO₃-N resulted in double the amount of exopolysaccharide production than in flasks with initial low loading-rates (less than 5 mg.L⁻¹ NO₃-N). Making use of an upflow anaerobic sludge blanket-type degrading-bed reactor, and an anaerobic, flooded trickle filter (ANTRIC) receiving HRAP effluent, the relationship between denitrification and the changes in polysaccharide content was investigated. This phenomenon has considerable beneficial implications in biological wastewater treatment systems where high nitrate concentration in the final effluent is a potential mitigating factor. Identification of the heterotrophic bacteria active in the denitrification process was attempted. This study presents a first report on the development and operation of the I-HRAP and has been followed by a technical-scale pilot plant evaluation of the process in the tertiary treatment of domestic wastewaters.
- Full Text:
- Date Issued: 2002
- Authors: Clark, Stewart James
- Date: 2002
- Subjects: Algae -- Biotechnology , Sewage -- Purification -- Biological treatment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4092 , http://hdl.handle.net/10962/d1007805
- Description: The development of the High Rate Algal Pond (HRAP) as an independent tertiary treatment unit operation for phosphate and nitrate removal is reported. A novel Integrated Algal Ponding System (lAPS) design is proposed for nutrient removal from the effluents of both a conventional domestic sewage treatment plant and from an Advanced Integrated Wastewater Ponding System (AIWPS). The viability of an independently operated HRAP has been identified and termed the Independent High Rate Algal Pond (l-HRAP). A 500 m² pilot 1- HRAP was operated in such a way as to facilitate the precipitation of calcium phosphate, known to be controlled by pH (greater than 9.4) and resulting in final phosphate levels of less than 1 mg.L⁻¹ as P0₄-P. The incorporation of the I-HRAP into a denitrification process was also investigated. Continuously fed column reactors, utilising algal biomass as a carbon source, showed that the heterotrophic bacterial community dominant in the anaerobic algal sludge were denitrifying the nitrate in the feed. It was demonstrated that as the cultures were stressed (using increased nitrate concentrations, anaerobiosis and light starvation) total polysaccharide (TPS) concentrations increased, with a notable increase 111 the exopolysaccharide (EPS) fraction. These experiments corroborated the hypothesis that harvested microalgal biomass can be manipulated to produce, and release, exopolymeric substances under stress conditions, and which may serve as carbon source for denitrification. In both batch flask studies and in laboratory-scale reactor systems, harvested microalgal biomass from an HRAP was shown to produce exopolymeric substances under stress conditions. Initial high loading-rates of greater than 20 mg.L⁻¹ NO₃-N resulted in double the amount of exopolysaccharide production than in flasks with initial low loading-rates (less than 5 mg.L⁻¹ NO₃-N). Making use of an upflow anaerobic sludge blanket-type degrading-bed reactor, and an anaerobic, flooded trickle filter (ANTRIC) receiving HRAP effluent, the relationship between denitrification and the changes in polysaccharide content was investigated. This phenomenon has considerable beneficial implications in biological wastewater treatment systems where high nitrate concentration in the final effluent is a potential mitigating factor. Identification of the heterotrophic bacteria active in the denitrification process was attempted. This study presents a first report on the development and operation of the I-HRAP and has been followed by a technical-scale pilot plant evaluation of the process in the tertiary treatment of domestic wastewaters.
- Full Text:
- Date Issued: 2002
The influence of structure, density and direct use benefits on the harvesting of trees in the rural village of Pikoi, Eastern Cape, South Africa
- Authors: Motinyane, Teboho R
- Date: 2002
- Subjects: Deforestation -- Control -- South Africa -- Eastern Cape , Deforestation -- South Africa -- Eastern Cape , Fuelwood consumption -- South Africa -- Eastern Cape , Trees -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4771 , http://hdl.handle.net/10962/d1007705 , Deforestation -- Control -- South Africa -- Eastern Cape , Deforestation -- South Africa -- Eastern Cape , Fuelwood consumption -- South Africa -- Eastern Cape , Trees -- South Africa -- Eastern Cape
- Description: The people of Pikoli in the Peddie district of the Eastern Cape are mostly unemployed and earn most of their income from old-age allowances. The place is remote and 'modern' amenities such as paraffin and petroleum gas are expensive. Electricity and transport are unavailable. There is heavy reliance on woody plants for fuel and for materials for construction and fencing and to some extent medicinal and ritual uses. Indigenous trees in this rural area are threatened by over-utilisation and the absence of clear government management policies. The over-utilisation of these resources is leading to deforestation. It was proposed at the beginning of this study that people who harvest trees cause similar results to the piosphere effect caused by the herbivores on the rangeland. Ecological-economic analysis was performed to find a common ground between the people's income and needs so that effective sustainable management strategies can be developed. A combination of semi-structured interviews, direct observations and ecological surveys were used to gather information about: Tree species used, and for what purpose in Pikoli; Individual tree species preference by inhabitants; Change in tree density in response to changes in utilisation intensity at varying distances away from the village; The local direct use benefits inhabitants derive from utilising indigenous tree resources. The study showed that the people of Pikoli use a variety of 23 indigenous trees and that they have marked preferences regarding the tree species they use for fuelwood and construction purposes. Although Ptaeroxylon obliquum is a highly preferred species for fuelwood and construction, its popularity can also be linked to its high relative abundance in this area. Other species such Maytenus undata and Pappea capensis are also highly preferred although they are harvested at much greater distances from thecentre of the village. Change in individual tree density in response to changes in utilisation intensity at varying distances away from the village showed clearly that a piosphere effect also applies to human harvesting. The average number of individual trees closer (1 200 m) to the village centre was 2 trees per 100 m², and their numbers increase to approximately 35 trees per 100 m² at a distance of about 3 000 m from the village centre. The study reports on the direct use benefits derived by the people of Pikoli from harvesting tree resources. The average total net value of trees harvested for fuelwood was R4089.09 (US$ 359.01) per household per annum. The value of tree resources harvested represents a large percentage of average households' annual income. Therefore, the economic benefits households derive from utilising tree resources are high. Indigenous tree resource use in this rural village seems to be unsustainable and the inhabitants seem to be aware of it. However, they have few alternatives. Their economic conditions do not allow them to afford alternative resources available in the formal market.
- Full Text:
- Date Issued: 2002
- Authors: Motinyane, Teboho R
- Date: 2002
- Subjects: Deforestation -- Control -- South Africa -- Eastern Cape , Deforestation -- South Africa -- Eastern Cape , Fuelwood consumption -- South Africa -- Eastern Cape , Trees -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4771 , http://hdl.handle.net/10962/d1007705 , Deforestation -- Control -- South Africa -- Eastern Cape , Deforestation -- South Africa -- Eastern Cape , Fuelwood consumption -- South Africa -- Eastern Cape , Trees -- South Africa -- Eastern Cape
- Description: The people of Pikoli in the Peddie district of the Eastern Cape are mostly unemployed and earn most of their income from old-age allowances. The place is remote and 'modern' amenities such as paraffin and petroleum gas are expensive. Electricity and transport are unavailable. There is heavy reliance on woody plants for fuel and for materials for construction and fencing and to some extent medicinal and ritual uses. Indigenous trees in this rural area are threatened by over-utilisation and the absence of clear government management policies. The over-utilisation of these resources is leading to deforestation. It was proposed at the beginning of this study that people who harvest trees cause similar results to the piosphere effect caused by the herbivores on the rangeland. Ecological-economic analysis was performed to find a common ground between the people's income and needs so that effective sustainable management strategies can be developed. A combination of semi-structured interviews, direct observations and ecological surveys were used to gather information about: Tree species used, and for what purpose in Pikoli; Individual tree species preference by inhabitants; Change in tree density in response to changes in utilisation intensity at varying distances away from the village; The local direct use benefits inhabitants derive from utilising indigenous tree resources. The study showed that the people of Pikoli use a variety of 23 indigenous trees and that they have marked preferences regarding the tree species they use for fuelwood and construction purposes. Although Ptaeroxylon obliquum is a highly preferred species for fuelwood and construction, its popularity can also be linked to its high relative abundance in this area. Other species such Maytenus undata and Pappea capensis are also highly preferred although they are harvested at much greater distances from thecentre of the village. Change in individual tree density in response to changes in utilisation intensity at varying distances away from the village showed clearly that a piosphere effect also applies to human harvesting. The average number of individual trees closer (1 200 m) to the village centre was 2 trees per 100 m², and their numbers increase to approximately 35 trees per 100 m² at a distance of about 3 000 m from the village centre. The study reports on the direct use benefits derived by the people of Pikoli from harvesting tree resources. The average total net value of trees harvested for fuelwood was R4089.09 (US$ 359.01) per household per annum. The value of tree resources harvested represents a large percentage of average households' annual income. Therefore, the economic benefits households derive from utilising tree resources are high. Indigenous tree resource use in this rural village seems to be unsustainable and the inhabitants seem to be aware of it. However, they have few alternatives. Their economic conditions do not allow them to afford alternative resources available in the formal market.
- Full Text:
- Date Issued: 2002
The molecular microbial ecology of sulfate reduction in the Rhodes BioSURE process
- Authors: Chauke, Chesa Gift
- Date: 2002
- Subjects: Water -- Purification -- Biological treatment , Acid mine drainage , Water -- Microbiology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4082 , http://hdl.handle.net/10962/d1007475 , Water -- Purification -- Biological treatment , Acid mine drainage , Water -- Microbiology
- Description: The research reported here investigated the use of a Baffle Reactor in order to study aspects of the biological sulfur cycle, where a floating sulfur biofilm formation occurs and where complex organic compounds provide electron donor sources. The development of a laboratory-scale Baffle Reactor model system satisfied the requirements for sulfate reducing bacterial biomass growth and sulfur biofilm formation. Since relatively little is known about the microbial ecology of floating sulfur biofilm systems, this study was undertaken to describe the sulfate reducing sludge population of the system together with its performance. A combination of culture- and molecular-based techniques were applied in this study in order to investigate the microbial ecology of the sulfate-reducing bacteria component of the system. These techniques enabled the identification and the analysis of the distribution of different sulfate reducing bacterial strains found within the sludge bioreactors. Strains isolated from the sludge were characterised based on culture appearance, gram staining and scanning electron microscopy morphology. Molecular methods based on the PCR-amplified 16S rRNA including denaturing gradient gel electrophoresis were employed in order to characterise sulfate-reducing bacteria within the reactors. Three novel Gram negative sulfate-reducing bacteria strains were isolated from the sludge population. Strains isolated were tentatively named Desulfomonas rhodensis, Desulfomonas makanaiensis, and Clostridium sulforhodensis. Results obtained from the Baffle Reactor showed that three dominant species were isolated from the DNA extracted from the whole bacterial population by peR. Three of these were similar to those mentioned above. The presence of these three novel unidentified species suggest that there are a range of other novel organisms involved in sulfate reduction processes.
- Full Text:
- Date Issued: 2002
- Authors: Chauke, Chesa Gift
- Date: 2002
- Subjects: Water -- Purification -- Biological treatment , Acid mine drainage , Water -- Microbiology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4082 , http://hdl.handle.net/10962/d1007475 , Water -- Purification -- Biological treatment , Acid mine drainage , Water -- Microbiology
- Description: The research reported here investigated the use of a Baffle Reactor in order to study aspects of the biological sulfur cycle, where a floating sulfur biofilm formation occurs and where complex organic compounds provide electron donor sources. The development of a laboratory-scale Baffle Reactor model system satisfied the requirements for sulfate reducing bacterial biomass growth and sulfur biofilm formation. Since relatively little is known about the microbial ecology of floating sulfur biofilm systems, this study was undertaken to describe the sulfate reducing sludge population of the system together with its performance. A combination of culture- and molecular-based techniques were applied in this study in order to investigate the microbial ecology of the sulfate-reducing bacteria component of the system. These techniques enabled the identification and the analysis of the distribution of different sulfate reducing bacterial strains found within the sludge bioreactors. Strains isolated from the sludge were characterised based on culture appearance, gram staining and scanning electron microscopy morphology. Molecular methods based on the PCR-amplified 16S rRNA including denaturing gradient gel electrophoresis were employed in order to characterise sulfate-reducing bacteria within the reactors. Three novel Gram negative sulfate-reducing bacteria strains were isolated from the sludge population. Strains isolated were tentatively named Desulfomonas rhodensis, Desulfomonas makanaiensis, and Clostridium sulforhodensis. Results obtained from the Baffle Reactor showed that three dominant species were isolated from the DNA extracted from the whole bacterial population by peR. Three of these were similar to those mentioned above. The presence of these three novel unidentified species suggest that there are a range of other novel organisms involved in sulfate reduction processes.
- Full Text:
- Date Issued: 2002
The role of cellulases and glucohydrolases in the solubilisation of primary sewage sludge
- Authors: Ngesi, Nosisa
- Date: 2002 , 2013-05-09
- Subjects: Sewage sludge , Sewage sludge digestion , Cellulase , Glucosidase inhibitors , Hydrolases , Sulfates
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4080 , http://hdl.handle.net/10962/d1007454 , Sewage sludge , Sewage sludge digestion , Cellulase , Glucosidase inhibitors , Hydrolases , Sulfates
- Description: Biological sulph ate reduction has been identi fied as a potentially valuable process for removing sulphate and heavy metals from indllstrial effluents. The role of sulphate reducing bacteria (SRB) in this process has attracted the attention of biotechnologists and recently of enzymologists due to its fundamental properties and possible role in AMD bioremediation. These obligatory anaerobic sulphate-reducing bacteria are commonly known to dissimilate sulphate for energy. Under anaerobic conditions SRB oxidize simple organic compounds such as lactic acid with the sulphate and thereby generate hydrogen sulphide (a stTong reducing agent) and bicarbonate ions. The hydrogen sulphide in turn reacts with contaminant metals contained in AMD and precipitates them out of solution as metal sulphides. Bicarbonate ions neutralize AMD by reaction with protons to form carbon dioxide and water. Organic matter in the municipal sewage sludge has been identified as a potential source of electron donors for su lphate reduction. However, this organic matter is in the polymeric form that cannot be util ised by SRB. The latter depend on the activities of other hydrolytic bacteria for the degradation of complex polymers. Hence the availability of these monomeric substrates is a major factor, which may constrain further process development and is considered a rate-limiting step. Thi s study is therefore undertaken to investigate the bacterial glucohydrolase enzymes involved in the digestion of the polysaccharides present in the sewage sludge with specific interest in cellulases and/or p-glucosidase enzymes. The goals of the research are to: isolate, identify, purify and quantify these enzymes; study their distribution with respect to time, pH, and temperature; maximize and quantify the hydrol ys is products; study whether sulphide and sulphate have an enhancing or an inhibitory effect on the activity of enzymes; optimize the enzyme activity against substrate and/or product inhibition and soluble heavy metal salts. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2002
- Authors: Ngesi, Nosisa
- Date: 2002 , 2013-05-09
- Subjects: Sewage sludge , Sewage sludge digestion , Cellulase , Glucosidase inhibitors , Hydrolases , Sulfates
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4080 , http://hdl.handle.net/10962/d1007454 , Sewage sludge , Sewage sludge digestion , Cellulase , Glucosidase inhibitors , Hydrolases , Sulfates
- Description: Biological sulph ate reduction has been identi fied as a potentially valuable process for removing sulphate and heavy metals from indllstrial effluents. The role of sulphate reducing bacteria (SRB) in this process has attracted the attention of biotechnologists and recently of enzymologists due to its fundamental properties and possible role in AMD bioremediation. These obligatory anaerobic sulphate-reducing bacteria are commonly known to dissimilate sulphate for energy. Under anaerobic conditions SRB oxidize simple organic compounds such as lactic acid with the sulphate and thereby generate hydrogen sulphide (a stTong reducing agent) and bicarbonate ions. The hydrogen sulphide in turn reacts with contaminant metals contained in AMD and precipitates them out of solution as metal sulphides. Bicarbonate ions neutralize AMD by reaction with protons to form carbon dioxide and water. Organic matter in the municipal sewage sludge has been identified as a potential source of electron donors for su lphate reduction. However, this organic matter is in the polymeric form that cannot be util ised by SRB. The latter depend on the activities of other hydrolytic bacteria for the degradation of complex polymers. Hence the availability of these monomeric substrates is a major factor, which may constrain further process development and is considered a rate-limiting step. Thi s study is therefore undertaken to investigate the bacterial glucohydrolase enzymes involved in the digestion of the polysaccharides present in the sewage sludge with specific interest in cellulases and/or p-glucosidase enzymes. The goals of the research are to: isolate, identify, purify and quantify these enzymes; study their distribution with respect to time, pH, and temperature; maximize and quantify the hydrol ys is products; study whether sulphide and sulphate have an enhancing or an inhibitory effect on the activity of enzymes; optimize the enzyme activity against substrate and/or product inhibition and soluble heavy metal salts. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2002
Thermal and photostability studies of furosemide and its cyclodextrin mixtures
- Authors: Melane, Babalwa Blossom
- Date: 2002 , 2013-05-16
- Subjects: Furosemide , Furosemide -- Stability
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4440 , http://hdl.handle.net/10962/d1007625 , Furosemide , Furosemide -- Stability
- Description: Furosemide (Lasix®), abbreviated as FR and also known as frusemide, is a drug used for renal problems and treatment of cardiac edema. Various polymorphic forms of furosemide, dependent upon the method of preparation and thermal treatment, have been reported. The main thermal decomposition product of furosemide has been identified as saluamine. The dissolution properties of furosemide have also been reported to be improved by complexation with beta-cyclodextrin. Photostabilities of the different crystal forms have been studied. Differential scanning calorimetry (DSC) and thermogravimetry (TG) have been used to examine the thermal behaviour of furosemide itself and of its physical and kneaded mixtures with betacyclodextrin (BCD) and gamma-cyclodextrin (GCD). There is strong evidence from DSC that complex formation between FR and GCD occurs. This is supported by IR and XRD data. Decreases in the intensity and broadening of the characteristic carbonyl (1660 cm'l) and amine (1588 cm⁻¹) bands in the kneaded mixture, compared to the physical mixture, were observed with IR. X-ray diffraction results for the 1:3 molar ratio FR/GCD kneaded mixture showed a halo diffraction pattern characteristic. of an amorphous solid and did not resemble patterns from the drug, or the gamma, cyclodextrin, or the physical mixture. Photostability studies have been conducted on solid furosemide and its mixtures with GCD or BCD. An HPLC method was developed to determine the amount of drug remaining after exposure and the presence of any degradants. Results indicated that about 10% degradation of the drug occurred during exposure for 16 hours at 550 W/m², with the appearance of polar degradants. Although IR and DSC results for the 1:3 molar ratio FR/GCD kneaded mixture showed a probable strong interaction between FR and GCD, the photostability of FR was decreased. The 1 :3 molar ratio FR/BCD kneaded mixture showed less photo-degradation than the 1:3 molar ratio FR/GCD mixture under similar conditions, suggesting that inclusion of the drug molecule (FR) is different in the two cyclodextrins.
- Full Text:
- Date Issued: 2002
- Authors: Melane, Babalwa Blossom
- Date: 2002 , 2013-05-16
- Subjects: Furosemide , Furosemide -- Stability
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4440 , http://hdl.handle.net/10962/d1007625 , Furosemide , Furosemide -- Stability
- Description: Furosemide (Lasix®), abbreviated as FR and also known as frusemide, is a drug used for renal problems and treatment of cardiac edema. Various polymorphic forms of furosemide, dependent upon the method of preparation and thermal treatment, have been reported. The main thermal decomposition product of furosemide has been identified as saluamine. The dissolution properties of furosemide have also been reported to be improved by complexation with beta-cyclodextrin. Photostabilities of the different crystal forms have been studied. Differential scanning calorimetry (DSC) and thermogravimetry (TG) have been used to examine the thermal behaviour of furosemide itself and of its physical and kneaded mixtures with betacyclodextrin (BCD) and gamma-cyclodextrin (GCD). There is strong evidence from DSC that complex formation between FR and GCD occurs. This is supported by IR and XRD data. Decreases in the intensity and broadening of the characteristic carbonyl (1660 cm'l) and amine (1588 cm⁻¹) bands in the kneaded mixture, compared to the physical mixture, were observed with IR. X-ray diffraction results for the 1:3 molar ratio FR/GCD kneaded mixture showed a halo diffraction pattern characteristic. of an amorphous solid and did not resemble patterns from the drug, or the gamma, cyclodextrin, or the physical mixture. Photostability studies have been conducted on solid furosemide and its mixtures with GCD or BCD. An HPLC method was developed to determine the amount of drug remaining after exposure and the presence of any degradants. Results indicated that about 10% degradation of the drug occurred during exposure for 16 hours at 550 W/m², with the appearance of polar degradants. Although IR and DSC results for the 1:3 molar ratio FR/GCD kneaded mixture showed a probable strong interaction between FR and GCD, the photostability of FR was decreased. The 1 :3 molar ratio FR/BCD kneaded mixture showed less photo-degradation than the 1:3 molar ratio FR/GCD mixture under similar conditions, suggesting that inclusion of the drug molecule (FR) is different in the two cyclodextrins.
- Full Text:
- Date Issued: 2002
Thermophilic lignin degrading enzymes from actinomycetes for biotechnological applications
- Authors: Mhlanga, Chido Yvonne Lois
- Date: 2002 , 2013-05-16
- Subjects: Actinomycetales -- Biotechnology , Lignin
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4085 , http://hdl.handle.net/10962/d1007628 , Actinomycetales -- Biotechnology , Lignin
- Description: Phenolic residues which accumulate in the environment as a result of agro-industrial practices has resulted in the need to find and use Eco-Friendly techniques, rather than the traditional methods of burning or burying this kind of waste. Bioremediation and bioconversion are attractive alternatives using whole cell or enzyme-based systems. The aims of this project were to isolate and uses thermophilic Actinomycetes, which produce thermo-tolerant oxidoreductase enzymes, which can be used to bioconvert a model industrial phenolic waste commonly genersated in the wine-making industry of South Africa. Current research in bioconversion and bioremediation focuses on mesophilic microbes in that their enzymes can catalyse reactions at higher temperatures without affecting its activity and lower contamination levels. Three novel Actinomycete isolates were isolated (RU-A0l , RU-A03 and RU-A06) from a compost site and characterized using a combination of conventional identification techniques and 16S rDNA methodology to identity the three isolates. All three isolates belong to the Streptomyces clade. In addition, five known Actinomycetes were selected from an internation culture collection and also screened for oxidoreductase activity in comparision to the three novel isolates. Although the five isolates were selected based on their ability to produce oxidoreductase enzymes, unexpectedly, no activity was detected. Screening assays for peroxidase, polyphenol oxidase and laccase on RU-AO 1, RU-A03 and RU-A06, showed that all three isolated produced peroxidases and peroxidases but no laccase. Substrate specificity studies revealed that the most suitable substrates to determine peroxidase and polyphenol oxidase activity on these isolates were catechol for polyphenol oxidase, 2,4-dichlorophenol for peroxidases and veratryl alcohol for lignin peroxidases. Previous studies have indicated that peroxidases and polyphenol oxidases are produced in Actinomycetes during the primary stage of growth. This was the case with RU-AOI , RU-A03 and RU-A06. Growth rates were higher that other Actinomycetes, with maxImum biomass being reached at 36 hours for the isolates RU-AOI and RU-A06 and 48 hours for isolate RUA03. pH studies showed that the three isolates were adaptable and could grow over a broad pH range. Catabolism studies of phenolic model compounds showed that the three isolates were capable of catabolizing the model phenolic compounds within a period of 24 hours. Further studies were carried out to determine the effect of these microbes and their enzymes in whole cell and enzyme-based systems on a model phenolic waste, graoe waste consisting of compressed grape skins, pips and stalks. Whole cell studies showed that the isolates were capable of bioconverting the waste at a maximum concentration of 30% grape waste (vol:vol). Peroxidase and polyphenol oxidase activity increased indicating induction of these enzymes in the presence of phenolic compounds, with a maximum increase of up to 15.9 fold increase in extracellular lignin peroxidase activity in RU-AO1. HPLC and phenolic determination assays indicated that bioconversion of the phenolic grape waste had occurred in the presence of the three isolates. Attempts were made to isolate and identify a peroxidase or phenol oxidase gene from one the isolates. As bacteria, Actinomycetes are amendable to gene manipulation making them suitable candidates for methods such as site directed evolution in comparison to fungi. Two clones were selected for sequencing based on positive activity results when assayed for peroxidase activity. However the resultant sequences did not identify a functional gene sequence. Southern Blotting was then carried out to determine the nature of the peroxidase gene. Previous studies have been focused on the catalase-peroxidase gene (CalC gene) found Actinomycetes and other bacteria. A probe was developed from the CalC gene. No hybridization occurred with any of the enzyme restricted DNA from the three isolates. The implications of these results are that the peroxidase genets in the three isolates are in fact lignin peroxidase in nature. This project has the potential in the bioconversion of phenolic wastes and is the first description of the use of thermophilic Actinomycetes in the bioconversion of an industrial phenolic waste.
- Full Text:
- Date Issued: 2002
- Authors: Mhlanga, Chido Yvonne Lois
- Date: 2002 , 2013-05-16
- Subjects: Actinomycetales -- Biotechnology , Lignin
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4085 , http://hdl.handle.net/10962/d1007628 , Actinomycetales -- Biotechnology , Lignin
- Description: Phenolic residues which accumulate in the environment as a result of agro-industrial practices has resulted in the need to find and use Eco-Friendly techniques, rather than the traditional methods of burning or burying this kind of waste. Bioremediation and bioconversion are attractive alternatives using whole cell or enzyme-based systems. The aims of this project were to isolate and uses thermophilic Actinomycetes, which produce thermo-tolerant oxidoreductase enzymes, which can be used to bioconvert a model industrial phenolic waste commonly genersated in the wine-making industry of South Africa. Current research in bioconversion and bioremediation focuses on mesophilic microbes in that their enzymes can catalyse reactions at higher temperatures without affecting its activity and lower contamination levels. Three novel Actinomycete isolates were isolated (RU-A0l , RU-A03 and RU-A06) from a compost site and characterized using a combination of conventional identification techniques and 16S rDNA methodology to identity the three isolates. All three isolates belong to the Streptomyces clade. In addition, five known Actinomycetes were selected from an internation culture collection and also screened for oxidoreductase activity in comparision to the three novel isolates. Although the five isolates were selected based on their ability to produce oxidoreductase enzymes, unexpectedly, no activity was detected. Screening assays for peroxidase, polyphenol oxidase and laccase on RU-AO 1, RU-A03 and RU-A06, showed that all three isolated produced peroxidases and peroxidases but no laccase. Substrate specificity studies revealed that the most suitable substrates to determine peroxidase and polyphenol oxidase activity on these isolates were catechol for polyphenol oxidase, 2,4-dichlorophenol for peroxidases and veratryl alcohol for lignin peroxidases. Previous studies have indicated that peroxidases and polyphenol oxidases are produced in Actinomycetes during the primary stage of growth. This was the case with RU-AOI , RU-A03 and RU-A06. Growth rates were higher that other Actinomycetes, with maxImum biomass being reached at 36 hours for the isolates RU-AOI and RU-A06 and 48 hours for isolate RUA03. pH studies showed that the three isolates were adaptable and could grow over a broad pH range. Catabolism studies of phenolic model compounds showed that the three isolates were capable of catabolizing the model phenolic compounds within a period of 24 hours. Further studies were carried out to determine the effect of these microbes and their enzymes in whole cell and enzyme-based systems on a model phenolic waste, graoe waste consisting of compressed grape skins, pips and stalks. Whole cell studies showed that the isolates were capable of bioconverting the waste at a maximum concentration of 30% grape waste (vol:vol). Peroxidase and polyphenol oxidase activity increased indicating induction of these enzymes in the presence of phenolic compounds, with a maximum increase of up to 15.9 fold increase in extracellular lignin peroxidase activity in RU-AO1. HPLC and phenolic determination assays indicated that bioconversion of the phenolic grape waste had occurred in the presence of the three isolates. Attempts were made to isolate and identify a peroxidase or phenol oxidase gene from one the isolates. As bacteria, Actinomycetes are amendable to gene manipulation making them suitable candidates for methods such as site directed evolution in comparison to fungi. Two clones were selected for sequencing based on positive activity results when assayed for peroxidase activity. However the resultant sequences did not identify a functional gene sequence. Southern Blotting was then carried out to determine the nature of the peroxidase gene. Previous studies have been focused on the catalase-peroxidase gene (CalC gene) found Actinomycetes and other bacteria. A probe was developed from the CalC gene. No hybridization occurred with any of the enzyme restricted DNA from the three isolates. The implications of these results are that the peroxidase genets in the three isolates are in fact lignin peroxidase in nature. This project has the potential in the bioconversion of phenolic wastes and is the first description of the use of thermophilic Actinomycetes in the bioconversion of an industrial phenolic waste.
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- Date Issued: 2002