The interactive effects of light, temperature and CO₂/O₂ ratios in photosynthesis of Coix lachryma-jobi L
- Authors: Mjwara, Jabulani Michael
- Date: 1992
- Subjects: Coix , Plants -- Effect of light on , Plants -- Effect of oxygen on , Plants -- Effect of carbon dioxide on , Photosynthesis , Plants -- Effect of temperature on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4216 , http://hdl.handle.net/10962/d1003785
- Description: A portable infra red gas analyzer was used to investigate the interactive effects of light, temperature, and CO,jO, ratios under controlled environmental conditions in an attempt to model gas exchange characteristics of Coix Iachryma-jobi L. Plotting light response curves as a function of temperature (20, 25 30 and 35°C) revealed no sign of light saturation even at a photosynthetic photon flux density (PPFD) close to 2000 !Lmol m-' sol. High net assimilation rates (A) of approximately 24 !Lmol CO, m"s'! were realized at 30-35°C. Assimilation (A) versus internal CO, partial pressure (C,) curves showed a steep rise with increase in C, but saturated at approximately 150 (JLII-!) and all the results, either in the absence or presence of 0" showed a similar response under all temperature regimes. C. Iachryma-jobi exhibited low CO, compensation points cr ) between 0 and 10 JLlI-! under similar experimental temperatures and either at 0 or 21%0,. The slopes of double reciprocal plots of llA versus llCi, were nearly identical and crossed the yintercept at almost identical points under all 0, concentrations. These data indicate first; that there was no apparent 0, inhibition and second; indicated that the apparent inhIbitor constant (K,) for 0, at the site of carboxylation did not change with increase in [OJ from 0 to 21% oxygen. These observations were further confirmed by results obtained from the analysis of apparent carboxylation efficiency (CE, as defined as the slope of response of A to increasing CO,), as no inhibition of A with increase of [OJ occurred. These characteristics were consistent with typical features of C,photosynthesis. The absence of 0, inhibition and low r values indicated that an efficient CO, concentrating mechanism which eliminates photorespiration exists in C. Iachryma-jobi. At the light microscope level, leaf anatomy exhibited typical C, structure viz. bundle sheath with large chloroplasts and this sheath is further surrounded by a radiate Kranz mesophyU cells. Furthermore the anatomical features suggested that C. wchryma-jobi was an NADP-ME species. Stomatal conductance (g,) to assimilation (g,/A) indicated an increase in A with decrease in g" an essential feature of improving water use efficiency (WUE), but one which drastically reduces CO, diffusion rate. The physical lintitation (stomatal lintitation, t) to CO, diffusion under various [0,] and temperatures, but constant PPFD, did not exhibit statistically significant change in t values at either 0 or 21% a, within each temperature regime, however there was a marked decrease in t as the plant approached its optimum photosynthetic temperature.
- Full Text:
- Date Issued: 1992
- Authors: Mjwara, Jabulani Michael
- Date: 1992
- Subjects: Coix , Plants -- Effect of light on , Plants -- Effect of oxygen on , Plants -- Effect of carbon dioxide on , Photosynthesis , Plants -- Effect of temperature on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4216 , http://hdl.handle.net/10962/d1003785
- Description: A portable infra red gas analyzer was used to investigate the interactive effects of light, temperature, and CO,jO, ratios under controlled environmental conditions in an attempt to model gas exchange characteristics of Coix Iachryma-jobi L. Plotting light response curves as a function of temperature (20, 25 30 and 35°C) revealed no sign of light saturation even at a photosynthetic photon flux density (PPFD) close to 2000 !Lmol m-' sol. High net assimilation rates (A) of approximately 24 !Lmol CO, m"s'! were realized at 30-35°C. Assimilation (A) versus internal CO, partial pressure (C,) curves showed a steep rise with increase in C, but saturated at approximately 150 (JLII-!) and all the results, either in the absence or presence of 0" showed a similar response under all temperature regimes. C. Iachryma-jobi exhibited low CO, compensation points cr ) between 0 and 10 JLlI-! under similar experimental temperatures and either at 0 or 21%0,. The slopes of double reciprocal plots of llA versus llCi, were nearly identical and crossed the yintercept at almost identical points under all 0, concentrations. These data indicate first; that there was no apparent 0, inhibition and second; indicated that the apparent inhIbitor constant (K,) for 0, at the site of carboxylation did not change with increase in [OJ from 0 to 21% oxygen. These observations were further confirmed by results obtained from the analysis of apparent carboxylation efficiency (CE, as defined as the slope of response of A to increasing CO,), as no inhibition of A with increase of [OJ occurred. These characteristics were consistent with typical features of C,photosynthesis. The absence of 0, inhibition and low r values indicated that an efficient CO, concentrating mechanism which eliminates photorespiration exists in C. Iachryma-jobi. At the light microscope level, leaf anatomy exhibited typical C, structure viz. bundle sheath with large chloroplasts and this sheath is further surrounded by a radiate Kranz mesophyU cells. Furthermore the anatomical features suggested that C. wchryma-jobi was an NADP-ME species. Stomatal conductance (g,) to assimilation (g,/A) indicated an increase in A with decrease in g" an essential feature of improving water use efficiency (WUE), but one which drastically reduces CO, diffusion rate. The physical lintitation (stomatal lintitation, t) to CO, diffusion under various [0,] and temperatures, but constant PPFD, did not exhibit statistically significant change in t values at either 0 or 21% a, within each temperature regime, however there was a marked decrease in t as the plant approached its optimum photosynthetic temperature.
- Full Text:
- Date Issued: 1992
The psychological sequelae of involvement in combat: a preliminary investigation
- Authors: Hodgson, Shane Ralph Colin
- Date: 1992
- Subjects: War -- Psychological aspects , War victims -- Mental health , Post-traumatic stress disorder
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:2993 , http://hdl.handle.net/10962/d1002502 , War -- Psychological aspects , War victims -- Mental health , Post-traumatic stress disorder
- Description: The psychological sequelae of being involved in combat are only recently coming to be understood. Most of the available data are from research conducted on help-seeking Vietnam veterans in the United States, and very little work has been done in South Africa. There does not as yet appear to be any instrument designed specifically to detect combat-related psychopathologies amongst soldiers who are still in active service, either in the USA or in South Africa. Combat involvement has been shown to lead to a high incidence of combat stress reaction. This in turn has shown that it can predispose sufferers to the development of a Post-traumatic Stress Disorder. It is thus expected that there would be significantly higher incidences of reported symptoms of stress disorders amongst soldiers exposed to high levels of combat as compared with a similar group of soldiers who had no combat involvement. This study used a self-reporting questionnaire, developed in the USA but adapted for use in South Africa, to allow the soldiers in the study to rate the severity of various symptoms derived from the DSM-III criteria for Post-traumatic Stress Disorder. A Beck Depression Inventory was also administered to eliminate any persons who nay have been exhibiting symptoms of depression, as this would have confounded the results. Both questionnaires were administered to serving members of the Permanent Force of the South African Defence Force, with one group being members of various high-combat units based in what was then South West Africa, and the other group being non-combat or Headquarters elements. As a precondition of the study, absolute confidentiality of the respondents and their units was maintained. The study found the expected higher scores in the high-combat group, and also showed that the Keane questionnaire has a good coefficient alpha in South Africa. The study closes with several recommendations for further research, especially in the light of the new PTSD criteria in the DSM-IIIR.
- Full Text:
- Date Issued: 1992
- Authors: Hodgson, Shane Ralph Colin
- Date: 1992
- Subjects: War -- Psychological aspects , War victims -- Mental health , Post-traumatic stress disorder
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:2993 , http://hdl.handle.net/10962/d1002502 , War -- Psychological aspects , War victims -- Mental health , Post-traumatic stress disorder
- Description: The psychological sequelae of being involved in combat are only recently coming to be understood. Most of the available data are from research conducted on help-seeking Vietnam veterans in the United States, and very little work has been done in South Africa. There does not as yet appear to be any instrument designed specifically to detect combat-related psychopathologies amongst soldiers who are still in active service, either in the USA or in South Africa. Combat involvement has been shown to lead to a high incidence of combat stress reaction. This in turn has shown that it can predispose sufferers to the development of a Post-traumatic Stress Disorder. It is thus expected that there would be significantly higher incidences of reported symptoms of stress disorders amongst soldiers exposed to high levels of combat as compared with a similar group of soldiers who had no combat involvement. This study used a self-reporting questionnaire, developed in the USA but adapted for use in South Africa, to allow the soldiers in the study to rate the severity of various symptoms derived from the DSM-III criteria for Post-traumatic Stress Disorder. A Beck Depression Inventory was also administered to eliminate any persons who nay have been exhibiting symptoms of depression, as this would have confounded the results. Both questionnaires were administered to serving members of the Permanent Force of the South African Defence Force, with one group being members of various high-combat units based in what was then South West Africa, and the other group being non-combat or Headquarters elements. As a precondition of the study, absolute confidentiality of the respondents and their units was maintained. The study found the expected higher scores in the high-combat group, and also showed that the Keane questionnaire has a good coefficient alpha in South Africa. The study closes with several recommendations for further research, especially in the light of the new PTSD criteria in the DSM-IIIR.
- Full Text:
- Date Issued: 1992
The status of the American bollworm, Heliothis armigera (Hubner) (Lepidoptera : Noctuidae), on sunflower in the central Transvaal
- Authors: Von Maltitz, Emil Friedrich
- Date: 1992
- Subjects: Helicoverpa armigera , Noctuidae -- South Africa -- Transvaal , Noctuidae , Sunflowers -- Diseases and pests
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5773 , http://hdl.handle.net/10962/d1005461 , Helicoverpa armigera , Noctuidae -- South Africa -- Transvaal , Noctuidae , Sunflowers -- Diseases and pests
- Description: Sunflower production in South Africa has increased four fold since the 1970's. This study was done to elucidate the pest status of Heliothis armigera (Hiibner) on sunflower. Field studies were undertaken at Warmbaths, Brits and Delmas during the summer seasons of 1988/89 to 1990/91. The infestations at the latter two areas were negligible throughout the study period and their statistics have not been included in this thesis. Plant development and oviposition by natural H. armigera populations were found to be correlated as, regardless of planting date, oviposition started at six to seven weeks after planting; when the flowering stage began. A peak in egg numbers was reached by the tenth week with an average for the three seasons at two eggs per plant. The eggs were laid singly on the bracts and bases of the flower buds. A peak in larval numbers was reached at the thirteenth week after planting with the average for the three seasons of 0,4 larvae per plant. The preferred feeding sites were on, between and under the bracts from where the larvae burrow into the pithy tissue of the receptacle. Only six percent of the larvae were found feeding directly on the achenes. Eggs and larvae collected were reared to determine the degree of parasitism. Overall, 19% of the eggs were parasitised; 18% by Telenomus ullyetti Nixon (Scelionidae) and one percent by Trichogrammatoidea lutea (Trichogrammatidae). Larval parasitism at Warmbaths was 23% in 1988/89, 27% in 1989/90 and 34% in 1990/91. Of the parasitised larvae, 44% succumbed to Palexorista prob. laxa (Tachinidae). The remainder were unidentified Braconidae and Ichneumonidae. Predators, such as chrysopids, were observed during the study but their effect on egg and larvae numbers was not studied in detail. A polyhedral virus occurred late in the seasons and caused mortality of the larvae. The low numbers of H. armigera on sunflower, the slight damage to the crop and the reasonably high rate of parasitism, all seem to indicate that H. armigera is not an economica1ly important pest of sunflower and that additional control methods are not justified.
- Full Text:
- Date Issued: 1992
- Authors: Von Maltitz, Emil Friedrich
- Date: 1992
- Subjects: Helicoverpa armigera , Noctuidae -- South Africa -- Transvaal , Noctuidae , Sunflowers -- Diseases and pests
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5773 , http://hdl.handle.net/10962/d1005461 , Helicoverpa armigera , Noctuidae -- South Africa -- Transvaal , Noctuidae , Sunflowers -- Diseases and pests
- Description: Sunflower production in South Africa has increased four fold since the 1970's. This study was done to elucidate the pest status of Heliothis armigera (Hiibner) on sunflower. Field studies were undertaken at Warmbaths, Brits and Delmas during the summer seasons of 1988/89 to 1990/91. The infestations at the latter two areas were negligible throughout the study period and their statistics have not been included in this thesis. Plant development and oviposition by natural H. armigera populations were found to be correlated as, regardless of planting date, oviposition started at six to seven weeks after planting; when the flowering stage began. A peak in egg numbers was reached by the tenth week with an average for the three seasons at two eggs per plant. The eggs were laid singly on the bracts and bases of the flower buds. A peak in larval numbers was reached at the thirteenth week after planting with the average for the three seasons of 0,4 larvae per plant. The preferred feeding sites were on, between and under the bracts from where the larvae burrow into the pithy tissue of the receptacle. Only six percent of the larvae were found feeding directly on the achenes. Eggs and larvae collected were reared to determine the degree of parasitism. Overall, 19% of the eggs were parasitised; 18% by Telenomus ullyetti Nixon (Scelionidae) and one percent by Trichogrammatoidea lutea (Trichogrammatidae). Larval parasitism at Warmbaths was 23% in 1988/89, 27% in 1989/90 and 34% in 1990/91. Of the parasitised larvae, 44% succumbed to Palexorista prob. laxa (Tachinidae). The remainder were unidentified Braconidae and Ichneumonidae. Predators, such as chrysopids, were observed during the study but their effect on egg and larvae numbers was not studied in detail. A polyhedral virus occurred late in the seasons and caused mortality of the larvae. The low numbers of H. armigera on sunflower, the slight damage to the crop and the reasonably high rate of parasitism, all seem to indicate that H. armigera is not an economica1ly important pest of sunflower and that additional control methods are not justified.
- Full Text:
- Date Issued: 1992
The surface geology of the Lavino Chrome Mine of the farm Grootboom 336KT, eastern Transvaal
- Authors: Tinney, Christopher Bruce
- Date: 1992
- Subjects: Chromium ores -- Geology -- South Africa -- Mpumalanga
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5067 , http://hdl.handle.net/10962/d1013404
- Description: A mapping project of the surface geology of the Lavino chrome mine and its surroundings was initiated in order to establish the surface geological relationships in the area. In so doing the chromitite layer presently being mined has been identified and potential exploration targets in the area have been outlined. The Lavino Chrome mine field area is situated within the eastern lobe of the Bushveld Igneous Complex. The area is bounded by in the north by the Steelpoort Lineament, in the west by the Dwars River fault and in the east by the contact with the Transvaal Sequence floor rocks. Layered igneous rocks (pyroxenites, norites and anorthosites) of the Rustenburg Layered Suite dominate the geological landscape at the Lavino mine. The fact that outcropping igneous rocks of the Critical Zone abut directly against the quartzite floor rocks on the mine property makes this area unique in the Bushveld Complex. The hills in the field area are capped by mafic/ultramafic iron-rich sheet - like bodies. Extensive strike-slip faulting is seen in outcrop in the area to the north/northwest of present mining operations. On the basis of field relationships, the main chromitite layer presently being mined at Lavino is identified as the Middle Group chromitite layer MG 1. Three other prominent chromitite layers stratigraphically associated with MG 1 are identified as the Middle Group chromitites MG 2, MG 3 and MG 4. Several other less prominent outcropping chromitite layers are tentatively identified as those belonging to the Lower and Upper group of chromitites. The disconformable nature of the contact between the layered igneous rocks and the Transvaal Sequence floor rocks has resulted in the development of a wedge of undifferentiated pyroxenites in the north of the field area. The economically important LG 6 chromitite layer may be developed in subcrop within this wedge.
- Full Text:
- Date Issued: 1992
- Authors: Tinney, Christopher Bruce
- Date: 1992
- Subjects: Chromium ores -- Geology -- South Africa -- Mpumalanga
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5067 , http://hdl.handle.net/10962/d1013404
- Description: A mapping project of the surface geology of the Lavino chrome mine and its surroundings was initiated in order to establish the surface geological relationships in the area. In so doing the chromitite layer presently being mined has been identified and potential exploration targets in the area have been outlined. The Lavino Chrome mine field area is situated within the eastern lobe of the Bushveld Igneous Complex. The area is bounded by in the north by the Steelpoort Lineament, in the west by the Dwars River fault and in the east by the contact with the Transvaal Sequence floor rocks. Layered igneous rocks (pyroxenites, norites and anorthosites) of the Rustenburg Layered Suite dominate the geological landscape at the Lavino mine. The fact that outcropping igneous rocks of the Critical Zone abut directly against the quartzite floor rocks on the mine property makes this area unique in the Bushveld Complex. The hills in the field area are capped by mafic/ultramafic iron-rich sheet - like bodies. Extensive strike-slip faulting is seen in outcrop in the area to the north/northwest of present mining operations. On the basis of field relationships, the main chromitite layer presently being mined at Lavino is identified as the Middle Group chromitite layer MG 1. Three other prominent chromitite layers stratigraphically associated with MG 1 are identified as the Middle Group chromitites MG 2, MG 3 and MG 4. Several other less prominent outcropping chromitite layers are tentatively identified as those belonging to the Lower and Upper group of chromitites. The disconformable nature of the contact between the layered igneous rocks and the Transvaal Sequence floor rocks has resulted in the development of a wedge of undifferentiated pyroxenites in the north of the field area. The economically important LG 6 chromitite layer may be developed in subcrop within this wedge.
- Full Text:
- Date Issued: 1992
The utilization of tilting-filter photometry in airglow and auroral research
- Authors: Dore, Ian Stuart
- Date: 1992
- Subjects: Photometry -- Research , Airglow -- Research , Auroras -- Research , Auroral photography -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5494 , http://hdl.handle.net/10962/d1005280 , Photometry -- Research , Airglow -- Research , Auroras -- Research , Auroral photography -- Research
- Description: This thesis describes the application of tilting-filter photometry to the study of the airglow and aurora. Previous South African photometric research is reviewed. Optical instrumentation and techniques used in airglow and auroral research are reviewed. The transmission characteristics of narrowband interference filters are discussed. The analogue meridian-scanning tilting-filter photometry system used at Sanae, Antarctica is described. Shortcomings of this system have been identified, and modifications have been made to improve its spatial and temporal resolution. Details are given of the computer-controlled digital photometry system which replaced the analogue system. Equations are derived for the conversion of raw photometric data (analogue chart deflections or digital photon counts) to absolute emission intensities. The accuracy of the intensities obtained depends on the absolute calibration of the photometer, the transmission characteristics of the filter used to isolate the spectral feature of interest, and the effects of atmospheric extinction and scattering. The influence of these factors on observed emission intensities is discussed. Various models used to determine atmospheric correction factors are reviewed. It is shown that atmospheric correction factors can have a significant effect on both emission intensities and intensity ratios. The procedure used to determine the transmission characteristics of interference filters is described, as is the procedure used to cross-calibrate secondary light sources. The transmission characteristics of the filters and the brightnesses of the light sources were both found to have changed appreciably with age. The observation of a magnetospheric substorm at Sanae (L ≃ 4) is used to illustrate the use of a meridian-scanning tilting-filter photometer system in auroral research. The ratio I(557.7)/ I(391.4) observed at Sanae was found to be lower than expected, as were the OJ airglow emission intensities. A prototype digital photometer system was used aboard a ship, to observe the airglow in the region of the South Atlantic Anomaly. Significant N₂⁺ lNG emissions at 391.4 nm were measured, confirming the presence of discernable particle precipitation in the region. The 0I557.7 and 630.0 nm intensities measured from the ship were found to be lower than expected. This, combined with low airglow and auroral intensities measured at Sanae, is a cause for concern. It is recommended that further checks be made regarding the brightness of the calibration sources.
- Full Text:
- Date Issued: 1992
- Authors: Dore, Ian Stuart
- Date: 1992
- Subjects: Photometry -- Research , Airglow -- Research , Auroras -- Research , Auroral photography -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5494 , http://hdl.handle.net/10962/d1005280 , Photometry -- Research , Airglow -- Research , Auroras -- Research , Auroral photography -- Research
- Description: This thesis describes the application of tilting-filter photometry to the study of the airglow and aurora. Previous South African photometric research is reviewed. Optical instrumentation and techniques used in airglow and auroral research are reviewed. The transmission characteristics of narrowband interference filters are discussed. The analogue meridian-scanning tilting-filter photometry system used at Sanae, Antarctica is described. Shortcomings of this system have been identified, and modifications have been made to improve its spatial and temporal resolution. Details are given of the computer-controlled digital photometry system which replaced the analogue system. Equations are derived for the conversion of raw photometric data (analogue chart deflections or digital photon counts) to absolute emission intensities. The accuracy of the intensities obtained depends on the absolute calibration of the photometer, the transmission characteristics of the filter used to isolate the spectral feature of interest, and the effects of atmospheric extinction and scattering. The influence of these factors on observed emission intensities is discussed. Various models used to determine atmospheric correction factors are reviewed. It is shown that atmospheric correction factors can have a significant effect on both emission intensities and intensity ratios. The procedure used to determine the transmission characteristics of interference filters is described, as is the procedure used to cross-calibrate secondary light sources. The transmission characteristics of the filters and the brightnesses of the light sources were both found to have changed appreciably with age. The observation of a magnetospheric substorm at Sanae (L ≃ 4) is used to illustrate the use of a meridian-scanning tilting-filter photometer system in auroral research. The ratio I(557.7)/ I(391.4) observed at Sanae was found to be lower than expected, as were the OJ airglow emission intensities. A prototype digital photometer system was used aboard a ship, to observe the airglow in the region of the South Atlantic Anomaly. Significant N₂⁺ lNG emissions at 391.4 nm were measured, confirming the presence of discernable particle precipitation in the region. The 0I557.7 and 630.0 nm intensities measured from the ship were found to be lower than expected. This, combined with low airglow and auroral intensities measured at Sanae, is a cause for concern. It is recommended that further checks be made regarding the brightness of the calibration sources.
- Full Text:
- Date Issued: 1992