The metabolic physiology of early stage Argyrosomus japonicus with insight into the potential effects of pCO2 induced ocean acidification
- Authors: Edworthy, Carla
- Date: 2018
- Subjects: Argyrosomus , Argyrosomus -- Growth , Argyrosomus -- Mortality , Argyrosomus -- Larvae -- Ecology , Ocean acidification , Marine ecology -- South Africa , Carbon dioxide -- Physiological effect
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/51417 , vital:26094
- Description: Ocean acidification is a phenomenon associated with global change and anthropogenic CO2 emissions that is changing the chemistry of seawater. These changes result in elevated pCO2 and reduced pH in seawater and this is impacting marine organisms in various ways. Marine fishes are considered generally tolerant to conditions of ocean acidification; however, these assumptions are based on juvenile and adult fish tolerance and the larval stages have not been frequently assessed. Furthermore, it has been suggested that temperate species, particularly those with an estuarine association, may be tolerant to variable CO2 and pH. This study used an eco-physiological approach to understand how the early life stages of Argyrosomus japonicus, an estuarine dependent marine fisheries species found in warm-temperate regions, may be impacted by ocean acidification. The metabolic response of early stage larvae (hatching to early juvenile stage) was assessed under conditions of elevated pCO2 and reduced pH in a controlled laboratory setting. Small volume static respirometry was used to determine the oxygen consumption rate of larvae raised in three pCO2 treatments including a low (pCO2 = 327.50 ± 80.07 µatm at pH 8.15), moderate (pCO2 477.40 ± 59.46 µatm at pH 8.03) and high treatment (PCO2 910.20 ± 136.45 µatm at pH 7.78). These treatment levels were relevant to the present (low) and projected conditions of ocean acidification for the years 2050 (moderate) and 2100 (high). Prior to experimentation with ocean acidification treatments, baseline metabolic rates and diurnal variation in oxygen consumption rates in early stage A. japonicus was determined. Distinct ontogenetic structuring of metabolic rates was observed in early stage A. japonicus, with no cyclical fluctuations in metabolic rate occurring during the 24 hour photoperiodic cycle. Pre-flexion larvae showed no metabolic response to ocean acidification treatments; however post-flexion stage larvae showed metabolic depression of standard metabolic rate in the moderate (32.5%) and high (9.5%) pCO2 treatments (P = 0.02). Larvae raised in the high pCO2 treatment also showed high levels of mortality with no individuals surviving past the post-flexion stage. Larvae raised in the moderate pCO2 treatment were unaffected. This study concluded that ocean acidification conditions expected for the end of the century will have significant impacts on the metabolism of early stage A. japonicus, which may result in reduced growth, retardation of skeletal development and ultimately survival as a result of increased mortality. Furthermore, the timing of reduced metabolic scope will significantly impact the recruitment ability of A. japonicus larvae into estuarine habitats. This could ultimately impact the sustainability of A. japonicus populations. Most importantly, this study highlighted the need to consider the combined effect of ontogeny and life-history strategy when assessing the vulnerability of species to ocean acidification.
- Full Text:
- Date Issued: 2018
- Authors: Edworthy, Carla
- Date: 2018
- Subjects: Argyrosomus , Argyrosomus -- Growth , Argyrosomus -- Mortality , Argyrosomus -- Larvae -- Ecology , Ocean acidification , Marine ecology -- South Africa , Carbon dioxide -- Physiological effect
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/51417 , vital:26094
- Description: Ocean acidification is a phenomenon associated with global change and anthropogenic CO2 emissions that is changing the chemistry of seawater. These changes result in elevated pCO2 and reduced pH in seawater and this is impacting marine organisms in various ways. Marine fishes are considered generally tolerant to conditions of ocean acidification; however, these assumptions are based on juvenile and adult fish tolerance and the larval stages have not been frequently assessed. Furthermore, it has been suggested that temperate species, particularly those with an estuarine association, may be tolerant to variable CO2 and pH. This study used an eco-physiological approach to understand how the early life stages of Argyrosomus japonicus, an estuarine dependent marine fisheries species found in warm-temperate regions, may be impacted by ocean acidification. The metabolic response of early stage larvae (hatching to early juvenile stage) was assessed under conditions of elevated pCO2 and reduced pH in a controlled laboratory setting. Small volume static respirometry was used to determine the oxygen consumption rate of larvae raised in three pCO2 treatments including a low (pCO2 = 327.50 ± 80.07 µatm at pH 8.15), moderate (pCO2 477.40 ± 59.46 µatm at pH 8.03) and high treatment (PCO2 910.20 ± 136.45 µatm at pH 7.78). These treatment levels were relevant to the present (low) and projected conditions of ocean acidification for the years 2050 (moderate) and 2100 (high). Prior to experimentation with ocean acidification treatments, baseline metabolic rates and diurnal variation in oxygen consumption rates in early stage A. japonicus was determined. Distinct ontogenetic structuring of metabolic rates was observed in early stage A. japonicus, with no cyclical fluctuations in metabolic rate occurring during the 24 hour photoperiodic cycle. Pre-flexion larvae showed no metabolic response to ocean acidification treatments; however post-flexion stage larvae showed metabolic depression of standard metabolic rate in the moderate (32.5%) and high (9.5%) pCO2 treatments (P = 0.02). Larvae raised in the high pCO2 treatment also showed high levels of mortality with no individuals surviving past the post-flexion stage. Larvae raised in the moderate pCO2 treatment were unaffected. This study concluded that ocean acidification conditions expected for the end of the century will have significant impacts on the metabolism of early stage A. japonicus, which may result in reduced growth, retardation of skeletal development and ultimately survival as a result of increased mortality. Furthermore, the timing of reduced metabolic scope will significantly impact the recruitment ability of A. japonicus larvae into estuarine habitats. This could ultimately impact the sustainability of A. japonicus populations. Most importantly, this study highlighted the need to consider the combined effect of ontogeny and life-history strategy when assessing the vulnerability of species to ocean acidification.
- Full Text:
- Date Issued: 2018
The potential significance of refugia in safeguarding Non-Timber Forest products under Harvesting
- Authors: Mjoli, Nwabisa
- Date: 2018
- Subjects: Non-timber forest products -- South Africa -- Eastern Cape , Nature conservation -- South Africa -- Eastern Cape , Ecosystem management -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/60596 , vital:27801
- Description: Many rural households living in and adjacent to forests in South Africa harvest a variety of NonTimber Forest Products (NTFPs) such as wild fruits, fibre, fuelwood, seeds, medicine and bush meat for domestic use, sale and maintaining cultural values. To promote the continued availability of these NTFPs it is important that NTFPs are well maintained and that harvest offtake is sustainable, because if it is not, then the important livelihood function that they fulfil will be jeopardized over time. The role of refugia in conserving pockets of threatened species from overharvesting has rarely been considered. Building on Shackleton et al. (2015), six applications of the concept are considered: size refugia, spatial refugia, cultural/spiritual refugia and physical/habitat refugia. I examined the prevalence and type of refugia for NTFPs at three coastal sites in the Eastern Cape province of South Africa. This was done via several community focus group discussions at each site. Here I report on the role of refugia in offering some safeguard to NTFPs by assessing harvested and non-harvested sites of refugia as well as individual plant species by measuring population density, harvest damage and size class profile. Respondents from the focus group discussions perceived a general decline in the abundance of common, widely used species over the past five decades. Decreasing rainfall and distant farming activities was reported consistently by the majority of the respondents. The most common form of refugia was spatial refugia, followed by cultural/spiritual refugia and physical/habitat refugia. Only six species were identified to be in refugia, namely Cyperus congestus, Cyperus textillis, Millettia grandis, Olea europaea subsp. africana, Phoenix reclinata, and Ptaeroxylon obliquum. Population inventories revealed that regeneration and population densities of some NTFP species in the non-refuge site were adversely affected by harvesting. Populations in refuge sites generally had the largest size classes indicating a regeneration potential but likely to impact regeneration over time if pressures are maintained. Therefore, if refuge sites keep larger individuals, NTFP populations can be maintained. Few studies have critically analyzed the concept of refugia in relation to how NTFPs can be safeguarded and the practical implication of the refuge concept needs to be considered in conservation and strategies and population models.
- Full Text:
- Date Issued: 2018
- Authors: Mjoli, Nwabisa
- Date: 2018
- Subjects: Non-timber forest products -- South Africa -- Eastern Cape , Nature conservation -- South Africa -- Eastern Cape , Ecosystem management -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/60596 , vital:27801
- Description: Many rural households living in and adjacent to forests in South Africa harvest a variety of NonTimber Forest Products (NTFPs) such as wild fruits, fibre, fuelwood, seeds, medicine and bush meat for domestic use, sale and maintaining cultural values. To promote the continued availability of these NTFPs it is important that NTFPs are well maintained and that harvest offtake is sustainable, because if it is not, then the important livelihood function that they fulfil will be jeopardized over time. The role of refugia in conserving pockets of threatened species from overharvesting has rarely been considered. Building on Shackleton et al. (2015), six applications of the concept are considered: size refugia, spatial refugia, cultural/spiritual refugia and physical/habitat refugia. I examined the prevalence and type of refugia for NTFPs at three coastal sites in the Eastern Cape province of South Africa. This was done via several community focus group discussions at each site. Here I report on the role of refugia in offering some safeguard to NTFPs by assessing harvested and non-harvested sites of refugia as well as individual plant species by measuring population density, harvest damage and size class profile. Respondents from the focus group discussions perceived a general decline in the abundance of common, widely used species over the past five decades. Decreasing rainfall and distant farming activities was reported consistently by the majority of the respondents. The most common form of refugia was spatial refugia, followed by cultural/spiritual refugia and physical/habitat refugia. Only six species were identified to be in refugia, namely Cyperus congestus, Cyperus textillis, Millettia grandis, Olea europaea subsp. africana, Phoenix reclinata, and Ptaeroxylon obliquum. Population inventories revealed that regeneration and population densities of some NTFP species in the non-refuge site were adversely affected by harvesting. Populations in refuge sites generally had the largest size classes indicating a regeneration potential but likely to impact regeneration over time if pressures are maintained. Therefore, if refuge sites keep larger individuals, NTFP populations can be maintained. Few studies have critically analyzed the concept of refugia in relation to how NTFPs can be safeguarded and the practical implication of the refuge concept needs to be considered in conservation and strategies and population models.
- Full Text:
- Date Issued: 2018
The role of ecological processes in structuring reef fish communities in the Agulhas Ecoregion, South Africa
- Authors: Dyer, Alexander
- Date: 2018
- Subjects: Reef fishes -- South Africa -- Agulhas, Cape (Cape) , Reef fishes -- Ecology -- South Africa -- Agulhas, Cape (Cape) , Marine biodiversity -- South Africa -- Agulhas, Cape (Cape) , Biotic communities -- South Africa -- Agulhas, Cape (Cape) , Sparidae -- South Africa -- Agulhas, Cape (Cape) , Reef fishes -- Size -- South Africa -- Agulhas, Cape (Cape) , Baited remote underwater stereo-video systems (stereo-BRUVs)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63899 , vital:28504
- Description: Local, niche-based processes, which arise from the interplay between biotic interactions and abiotic constraints are considered to be important regulators of community structure. However, it is increasingly recognised that patterns of diversity can also be strongly influenced by dispersal-driven processes. While empirical research on the diversity of coral reef fishes on shallow tropical reefs has contributed greatly to the development of general concepts in ecology, there have been considerably fewer studies on the processes which shape the diversity of fish communities on shallow (10 - 30 m) and deep (30 - 75 m) rocky reefs. Consequently, it is less clear at which spatial scales niche partitioning and dispersal limitation contribute most strongly to the structure of reef-associated fish communities within rocky reef ecosystems. To address this caveat, research was conducted at four rocky reef complexes within the warm-temperate Agulhas Ecoregion, South Africa. The diversity of reef-associated fishes was sampled by baited remote underwater stereo-video systems (stereo-BRUVs) to incorporate the range of heterogeneous reef habitat in Tsitsikamma National Park Marine Protected Area (TNP MPA) and Algoa Bay (AB). To examine how niche-based and dispersal-driven processes influence patterns of diversity among species within the dominant family of resident fishes, the sparids (Sparidae), components of diversity were quantified at several spatial scales. Turnover in the number of species which locally co-occurred was found to be largely driven by the limited dispersal of species over hundreds of kilometres. When relative species abundances were taken into account, sparid communities were characterised by higher than expected rates of compositional turnover among local habitat patches separated by hundreds to thousands of metres of contiguous reef. Patterns of compositional turnover were associated with the spatial aggregation of conspecifics, particularly at scales which facilitate the post-settlement dispersal of fishes. Niche-based segregation of species along the depth gradient was found to be the primary driver of compositional turnover among both protected and exploited communities. However, spatial structuring within reefs, which was independent of variation in the environment, suggests that compositional differences among communities are also influenced by the limited post-settlement dispersal of resident fishes to adjacent areas during their ontogeny. Together, the results suggest that the diversity of reef-associated sparids is likely to depend both on an adequate diversity of suitable reef habitat and a sufficient degree of spatial connectivity to facilitate ontogenetic habitat shifts. Taxon-based descriptors of diversity do not adequately account for ecological difference among conspecifics within size-structured populations. To test whether differences in body size facilitated coexistence among sparid fishes, the number of species which coexisted at the local scale was related to variation in the size structure of communities. In communities which have been historically protected from fishing, local coexistence between a greater number of species was promoted by reduced levels of intraspecific variation in size of fishes. This suggests that, among species with similar trophic requirements, further niche segregation along a prey-size and body-size gradient is likely to mitigate the direct impacts of competition for shared food resources. Among exploited communities, size structure did not influence the number of species which coexisted at the local scale. This finding suggests that fishing-induced mortality of larger-bodied fishes is likely to remove some of the constraints to colonisation which arise from asymmetries in competitive fitness between small and large-bodied fishes. Together, these results highlight the importance of post-settlement processes and population size structure to the maintenance of reef-associated fish diversity within contiguous rocky reef habitats.
- Full Text:
- Date Issued: 2018
- Authors: Dyer, Alexander
- Date: 2018
- Subjects: Reef fishes -- South Africa -- Agulhas, Cape (Cape) , Reef fishes -- Ecology -- South Africa -- Agulhas, Cape (Cape) , Marine biodiversity -- South Africa -- Agulhas, Cape (Cape) , Biotic communities -- South Africa -- Agulhas, Cape (Cape) , Sparidae -- South Africa -- Agulhas, Cape (Cape) , Reef fishes -- Size -- South Africa -- Agulhas, Cape (Cape) , Baited remote underwater stereo-video systems (stereo-BRUVs)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63899 , vital:28504
- Description: Local, niche-based processes, which arise from the interplay between biotic interactions and abiotic constraints are considered to be important regulators of community structure. However, it is increasingly recognised that patterns of diversity can also be strongly influenced by dispersal-driven processes. While empirical research on the diversity of coral reef fishes on shallow tropical reefs has contributed greatly to the development of general concepts in ecology, there have been considerably fewer studies on the processes which shape the diversity of fish communities on shallow (10 - 30 m) and deep (30 - 75 m) rocky reefs. Consequently, it is less clear at which spatial scales niche partitioning and dispersal limitation contribute most strongly to the structure of reef-associated fish communities within rocky reef ecosystems. To address this caveat, research was conducted at four rocky reef complexes within the warm-temperate Agulhas Ecoregion, South Africa. The diversity of reef-associated fishes was sampled by baited remote underwater stereo-video systems (stereo-BRUVs) to incorporate the range of heterogeneous reef habitat in Tsitsikamma National Park Marine Protected Area (TNP MPA) and Algoa Bay (AB). To examine how niche-based and dispersal-driven processes influence patterns of diversity among species within the dominant family of resident fishes, the sparids (Sparidae), components of diversity were quantified at several spatial scales. Turnover in the number of species which locally co-occurred was found to be largely driven by the limited dispersal of species over hundreds of kilometres. When relative species abundances were taken into account, sparid communities were characterised by higher than expected rates of compositional turnover among local habitat patches separated by hundreds to thousands of metres of contiguous reef. Patterns of compositional turnover were associated with the spatial aggregation of conspecifics, particularly at scales which facilitate the post-settlement dispersal of fishes. Niche-based segregation of species along the depth gradient was found to be the primary driver of compositional turnover among both protected and exploited communities. However, spatial structuring within reefs, which was independent of variation in the environment, suggests that compositional differences among communities are also influenced by the limited post-settlement dispersal of resident fishes to adjacent areas during their ontogeny. Together, the results suggest that the diversity of reef-associated sparids is likely to depend both on an adequate diversity of suitable reef habitat and a sufficient degree of spatial connectivity to facilitate ontogenetic habitat shifts. Taxon-based descriptors of diversity do not adequately account for ecological difference among conspecifics within size-structured populations. To test whether differences in body size facilitated coexistence among sparid fishes, the number of species which coexisted at the local scale was related to variation in the size structure of communities. In communities which have been historically protected from fishing, local coexistence between a greater number of species was promoted by reduced levels of intraspecific variation in size of fishes. This suggests that, among species with similar trophic requirements, further niche segregation along a prey-size and body-size gradient is likely to mitigate the direct impacts of competition for shared food resources. Among exploited communities, size structure did not influence the number of species which coexisted at the local scale. This finding suggests that fishing-induced mortality of larger-bodied fishes is likely to remove some of the constraints to colonisation which arise from asymmetries in competitive fitness between small and large-bodied fishes. Together, these results highlight the importance of post-settlement processes and population size structure to the maintenance of reef-associated fish diversity within contiguous rocky reef habitats.
- Full Text:
- Date Issued: 2018
The role of field and garden cultivation for food security under a changing climate: the case of Fairbairn and Ntloko villages, Eastern Cape
- Authors: Brooks, Haydn
- Date: 2018
- Subjects: Climatic changes -- Economic aspects -- Eastern Cape (South Africa) , Climatic changes -- Social aspects -- South Africa , Food -- Economic aspects -- South Africa , Food security -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/58013 , vital:27019
- Description: The world is becoming more complex due to the increasing occurrence of social-ecological change. This is particularly evident in the developing world, especially on the African continent, where existing vulnerabilities (i.e. endemic poverty, weak governance, overpopulation and HIV/AIDS) are being exacerbated by the impact of climate change. Climate change poses a very real threat to millions of Africans, especially those who rely on the natural world for their livelihoods. The increasing variability of climate and rainfall patterns are said to have dire consequences on agricultural production which is the main livelihood activity of rural dwellers across the continent. The impacts of such change and variability include, changes in the frequency and intensity of droughts, flooding and heavy storms, leading to worsening soil conditions, desertification as well as disease and pest out-breaks which will likely result in reduced crop and livestock yields. A reduction in agricultural production will likely have a negative effect on the food security of millions of people. This study was conducted in the Eastern Cape of South Africa, in two rural villages, Fairbairn and Ntloko located in the former Ciskei homeland, with the purpose of exploring the nexus between climate change, cultivation and food security. This study combined social-ecological thinking with that of political ecology to create a robust lens, in which to analyse the complex interactions between humans and the environment. A mixed methods approach was used to gather the data which consisted of a household survey, using a structured questionnaire as well as semi-structured interviews with various respondents. Quantitative data were analysed using Excel and Statistica 13, whilst coding was used for qualitative data. The main aim of the study was to explore the extent and characteristics of cultivation in the two study sites, the role of household food production in food security as well as the challenges that local cultivator’s face with regards to climate change. In recent decades, there has been a decline in rural agricultural production with many fields that were previously cultivated, lying vacant and unused. However, as this study found, cultivation is still important as over 50% of sampled households cultivated a small area within the homestead. This was mainly done to supplement household food expenditure in order to save cash incomes. The results also found that although participants had perceived negative changes in climate and rainfall (amount, onset, duration), it was not considered the most important challenge for own production. Rather, lack of fencing and capital were seen as major hindrances to efficient own production. Understanding the complexity of own production and food security under a changing climate can help form better and more resilient policies and strategies for rural development, in addition to strengthening future livelihoods of rural people in an ever changing world.
- Full Text:
- Date Issued: 2018
- Authors: Brooks, Haydn
- Date: 2018
- Subjects: Climatic changes -- Economic aspects -- Eastern Cape (South Africa) , Climatic changes -- Social aspects -- South Africa , Food -- Economic aspects -- South Africa , Food security -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/58013 , vital:27019
- Description: The world is becoming more complex due to the increasing occurrence of social-ecological change. This is particularly evident in the developing world, especially on the African continent, where existing vulnerabilities (i.e. endemic poverty, weak governance, overpopulation and HIV/AIDS) are being exacerbated by the impact of climate change. Climate change poses a very real threat to millions of Africans, especially those who rely on the natural world for their livelihoods. The increasing variability of climate and rainfall patterns are said to have dire consequences on agricultural production which is the main livelihood activity of rural dwellers across the continent. The impacts of such change and variability include, changes in the frequency and intensity of droughts, flooding and heavy storms, leading to worsening soil conditions, desertification as well as disease and pest out-breaks which will likely result in reduced crop and livestock yields. A reduction in agricultural production will likely have a negative effect on the food security of millions of people. This study was conducted in the Eastern Cape of South Africa, in two rural villages, Fairbairn and Ntloko located in the former Ciskei homeland, with the purpose of exploring the nexus between climate change, cultivation and food security. This study combined social-ecological thinking with that of political ecology to create a robust lens, in which to analyse the complex interactions between humans and the environment. A mixed methods approach was used to gather the data which consisted of a household survey, using a structured questionnaire as well as semi-structured interviews with various respondents. Quantitative data were analysed using Excel and Statistica 13, whilst coding was used for qualitative data. The main aim of the study was to explore the extent and characteristics of cultivation in the two study sites, the role of household food production in food security as well as the challenges that local cultivator’s face with regards to climate change. In recent decades, there has been a decline in rural agricultural production with many fields that were previously cultivated, lying vacant and unused. However, as this study found, cultivation is still important as over 50% of sampled households cultivated a small area within the homestead. This was mainly done to supplement household food expenditure in order to save cash incomes. The results also found that although participants had perceived negative changes in climate and rainfall (amount, onset, duration), it was not considered the most important challenge for own production. Rather, lack of fencing and capital were seen as major hindrances to efficient own production. Understanding the complexity of own production and food security under a changing climate can help form better and more resilient policies and strategies for rural development, in addition to strengthening future livelihoods of rural people in an ever changing world.
- Full Text:
- Date Issued: 2018
The spatial ecology of Albula glossodonta in the St. Joseph Atoll, Seychelles
- Authors: Moxham, Emily Jeanne
- Date: 2018
- Subjects: Bonefish -- Seychelles -- Amirante Islands , Albula -- Seychelles -- Amirante Islands , Bonefish fisheries -- Seychelles -- Amirante Islands , Small-scale fisheries -- Seychelles -- Amirante Islands -- Economic conditions , Underwater acoustic telemetry , Bonefish -- Behavior -- Seychelles -- Amirante Islands , Bonefish -- Home range -- Seychelles -- Amirante Islands , Bonefish -- Conservation -- Seychelles -- Amirante Islands , Predation (Biology) -- Seychelles -- Amirante Islands , Bonefishing
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/57863 , vital:26997
- Description: Bonefish (Albula spp.) support valuable recreational and artisanal fisheries worldwide. Declining stocks have been reported at multiple localities, potentially jeopardising numerous multimillion-dollar industries. In particular, tourism generated through bonefish fly fishing contributes considerably to the economies of many isolated tropical islands and atolls. However, despite their economic value, little is known about bonefish in the Indian Ocean. This study aimed to contribute to the understanding of bonefish ecology in the Indian Ocean by (1) reviewing the bonefish literature to identify knowledge gaps; (2) evaluating the postrelease survival of acoustically tagged bonefish and; (3) quantifying the spatial and temporal movements of bonefish at a near-pristine and predator rich atoll in the Seychelles. A review of published literature on bonefish indicated that despite considerable biological and ecological research in the Pacific and Atlantic oceans, virtually no research has been conducted in the Indian Ocean. To help address this research gap, an acoustic telemetry study was initiated at the remote St. Joseph Atoll, within an existing array of 88 automated datalogging acoustic receivers. Thirty Albula glossodonta were surgically implanted with Vemco V13 acoustic transmitters in May 2015 and tracked for a period of one year. Only 10% of the tagged bonefish were detected for more than two weeks. A comparison of the final 100 hours of movement data from fish that were detected for less than two weeks to fish detected for longer periods revealed distinct differences. These included differences in area use patterns and significant differences in the average daily distance moved, speed of movement and residency index. This suggested that mortality in the form of post-release predation was high (90%) with tagged fish detected for less than two weeks being preyed upon by sharks. The three surviving bonefish were tracked for 210 to 367 days. These individuals remained in the atoll and showed high use of the marginal habitats between the shallow sand flats and the deeper lagoon. Water temperature, diel cycle and tide were significant predictors of bonefish presence in the lagoon. The high post-release predation of bonefish has implications for the management of this and other Albula species. Despite these fisheries being catch-and-release, bonefish fishing may be unsustainable due to the high post-release mortality, particularly in areas that are rich in predators. Therefore, protected areas or limitations on fishing effort need to be considered.
- Full Text:
- Date Issued: 2018
- Authors: Moxham, Emily Jeanne
- Date: 2018
- Subjects: Bonefish -- Seychelles -- Amirante Islands , Albula -- Seychelles -- Amirante Islands , Bonefish fisheries -- Seychelles -- Amirante Islands , Small-scale fisheries -- Seychelles -- Amirante Islands -- Economic conditions , Underwater acoustic telemetry , Bonefish -- Behavior -- Seychelles -- Amirante Islands , Bonefish -- Home range -- Seychelles -- Amirante Islands , Bonefish -- Conservation -- Seychelles -- Amirante Islands , Predation (Biology) -- Seychelles -- Amirante Islands , Bonefishing
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/57863 , vital:26997
- Description: Bonefish (Albula spp.) support valuable recreational and artisanal fisheries worldwide. Declining stocks have been reported at multiple localities, potentially jeopardising numerous multimillion-dollar industries. In particular, tourism generated through bonefish fly fishing contributes considerably to the economies of many isolated tropical islands and atolls. However, despite their economic value, little is known about bonefish in the Indian Ocean. This study aimed to contribute to the understanding of bonefish ecology in the Indian Ocean by (1) reviewing the bonefish literature to identify knowledge gaps; (2) evaluating the postrelease survival of acoustically tagged bonefish and; (3) quantifying the spatial and temporal movements of bonefish at a near-pristine and predator rich atoll in the Seychelles. A review of published literature on bonefish indicated that despite considerable biological and ecological research in the Pacific and Atlantic oceans, virtually no research has been conducted in the Indian Ocean. To help address this research gap, an acoustic telemetry study was initiated at the remote St. Joseph Atoll, within an existing array of 88 automated datalogging acoustic receivers. Thirty Albula glossodonta were surgically implanted with Vemco V13 acoustic transmitters in May 2015 and tracked for a period of one year. Only 10% of the tagged bonefish were detected for more than two weeks. A comparison of the final 100 hours of movement data from fish that were detected for less than two weeks to fish detected for longer periods revealed distinct differences. These included differences in area use patterns and significant differences in the average daily distance moved, speed of movement and residency index. This suggested that mortality in the form of post-release predation was high (90%) with tagged fish detected for less than two weeks being preyed upon by sharks. The three surviving bonefish were tracked for 210 to 367 days. These individuals remained in the atoll and showed high use of the marginal habitats between the shallow sand flats and the deeper lagoon. Water temperature, diel cycle and tide were significant predictors of bonefish presence in the lagoon. The high post-release predation of bonefish has implications for the management of this and other Albula species. Despite these fisheries being catch-and-release, bonefish fishing may be unsustainable due to the high post-release mortality, particularly in areas that are rich in predators. Therefore, protected areas or limitations on fishing effort need to be considered.
- Full Text:
- Date Issued: 2018
The thermal physiology of Stenopelmus rufinasus and Neohydronomus affinis (Coleoptera: Curculionidae), biological control agents for the invasive alien aquatic weeds Azolla filiculoides and Pistia stratiotes respectively
- Authors: Mvandaba, Sisanda F
- Date: 2018
- Subjects: Beetles -- South Africa , Curculionidae -- South Africa , Azolla filiculoides -- South Africa , Water lettuce -- South Africa , Aquatic weeds -- Biological control -- South Africa , Stenopelmus rufinasus , Neohydronomus affinis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62362 , vital:28158
- Description: Water lettuce, Pistia stratiotes L. (Araceae), and red water fern, Azolla filiculoides Lam. (Azollaceae), are floating aquatic macrophytes that have become problematic invaders in numerous South African waterbodies. Two weevils, Neohydronomus affinis Hustache 1926 (Coleoptera: Curculionidae) and Stenopelmus rufinasus Gyllenhal 1936 (Coleoptera: Curculionidae), are successful biological control agents of these two species, respectively, in South Africa. However, nothing is known about the thermal physiology of these two species Therefore, the aim of this study was to investigate the thermal physiologies of these two species to explain their establishment, distribution and impact in the field. Laboratory based thermal physiology trials showed that both weevils were widely tolerant of cold and warm temperatures. The CTmin of N. affinis was determined to be 5.5 ± 0.312°C and the CTmax was 44 ± 0.697°C, while the CTmin of S. rufinasus was 5.4 ± 0.333°C and the CTmax was 44.5 ± 0.168°C. In addition, the lower lethal temperatures were -9.8 ± 0.053°C and -7.2 ± 0.19°C, and the upper lethal temperatures were 42.8 ± 0.053°C and 41.9 ± 0.19°C respectively. These results suggest that both species should not be limited by cold winter temperatures, as previously thought. This is evident in the field, where S. rufinasus has established widely on A. filiculoides, despite local cold climates in some areas of the plant’s distribution. Even though N. affinis has a similar thermal range, and should therefore theoretically reflect a similar distribution to S. rufinasus throughout South Africa, its distribution is limited by the range of its host, which is restricted to the warmer regions of the country, as is its biocontrol agent. Using the reduced major axis regression method, the development for N. affinis was described using the formulay=12.976x+435.24, while the development of S. rufinasus was described by y=13.6x+222.45. These results showed that S. rufinasus develops much faster, in fact almost twice as quickly, than N. affinis. Using these formulae and temperature data obtained from the South African Weather Service, N. affinis was predicted to complete between 4 and 9 generations per year in South Africa, while S. rufinasus was predicted to complete between 5 and 14 generations per year around the country. This study showed that although the native range of these two species is warm temperate to tropical, they possess sufficient thermal plasticity to not only establish, but also damage their respective host plants in far cooler climates. Thus, in South Africa N. affinis and S. rufinasus are limited by the distribution of their target weeds and not climate.
- Full Text:
- Date Issued: 2018
- Authors: Mvandaba, Sisanda F
- Date: 2018
- Subjects: Beetles -- South Africa , Curculionidae -- South Africa , Azolla filiculoides -- South Africa , Water lettuce -- South Africa , Aquatic weeds -- Biological control -- South Africa , Stenopelmus rufinasus , Neohydronomus affinis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62362 , vital:28158
- Description: Water lettuce, Pistia stratiotes L. (Araceae), and red water fern, Azolla filiculoides Lam. (Azollaceae), are floating aquatic macrophytes that have become problematic invaders in numerous South African waterbodies. Two weevils, Neohydronomus affinis Hustache 1926 (Coleoptera: Curculionidae) and Stenopelmus rufinasus Gyllenhal 1936 (Coleoptera: Curculionidae), are successful biological control agents of these two species, respectively, in South Africa. However, nothing is known about the thermal physiology of these two species Therefore, the aim of this study was to investigate the thermal physiologies of these two species to explain their establishment, distribution and impact in the field. Laboratory based thermal physiology trials showed that both weevils were widely tolerant of cold and warm temperatures. The CTmin of N. affinis was determined to be 5.5 ± 0.312°C and the CTmax was 44 ± 0.697°C, while the CTmin of S. rufinasus was 5.4 ± 0.333°C and the CTmax was 44.5 ± 0.168°C. In addition, the lower lethal temperatures were -9.8 ± 0.053°C and -7.2 ± 0.19°C, and the upper lethal temperatures were 42.8 ± 0.053°C and 41.9 ± 0.19°C respectively. These results suggest that both species should not be limited by cold winter temperatures, as previously thought. This is evident in the field, where S. rufinasus has established widely on A. filiculoides, despite local cold climates in some areas of the plant’s distribution. Even though N. affinis has a similar thermal range, and should therefore theoretically reflect a similar distribution to S. rufinasus throughout South Africa, its distribution is limited by the range of its host, which is restricted to the warmer regions of the country, as is its biocontrol agent. Using the reduced major axis regression method, the development for N. affinis was described using the formulay=12.976x+435.24, while the development of S. rufinasus was described by y=13.6x+222.45. These results showed that S. rufinasus develops much faster, in fact almost twice as quickly, than N. affinis. Using these formulae and temperature data obtained from the South African Weather Service, N. affinis was predicted to complete between 4 and 9 generations per year in South Africa, while S. rufinasus was predicted to complete between 5 and 14 generations per year around the country. This study showed that although the native range of these two species is warm temperate to tropical, they possess sufficient thermal plasticity to not only establish, but also damage their respective host plants in far cooler climates. Thus, in South Africa N. affinis and S. rufinasus are limited by the distribution of their target weeds and not climate.
- Full Text:
- Date Issued: 2018
Towards a collection of cost-effective technologies in support of the NIST cybersecurity framework
- Shackleton, Bruce Michael Stuart
- Authors: Shackleton, Bruce Michael Stuart
- Date: 2018
- Subjects: National Institute of Standards and Technology (U.S.) , Computer security , Computer networks Security measures , Small business Information technology Cost effectiveness , Open source software
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62494 , vital:28199
- Description: The NIST Cybersecurity Framework (CSF) is a specific risk and cybersecurity framework. It provides guidance on controls that can be implemented to help improve an organisation’s cybersecurity risk posture. The CSF Functions consist of Identify, Protect, Detect, Respond, and Recover. Like most Information Technology (IT) frameworks, there are elements of people, processes, and technology. The same elements are required to successfully implement the NIST CSF. This research specifically focuses on the technology element. While there are many commercial technologies available for a small to medium sized business, the costs can be prohibitively expensive. Therefore, this research investigates cost-effective technologies and assesses their alignment to the NIST CSF. The assessment was made against the NIST CSF subcategories. Each subcategory was analysed to identify where a technology would likely be required. The framework provides a list of Informative References. These Informative References were used to create high- level technology categories, as well as identify the technical controls against which the technologies were measured. The technologies tested were either open source or proprietary. All open source technologies tested were free to use, or have a free community edition. Proprietary technologies would be free to use, or considered generally available to most organisations, such as components contained within Microsoft platforms. The results from the experimentation demonstrated that there are multiple cost-effective technologies that can support the NIST CSF. Once all technologies were tested, the NIST CSF was extended. Two new columns were added, namely high-level technology category, and tested technology. The columns were populated with output from the research. This extended framework begins an initial collection of cost-effective technologies in support of the NIST CSF.
- Full Text:
- Date Issued: 2018
- Authors: Shackleton, Bruce Michael Stuart
- Date: 2018
- Subjects: National Institute of Standards and Technology (U.S.) , Computer security , Computer networks Security measures , Small business Information technology Cost effectiveness , Open source software
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62494 , vital:28199
- Description: The NIST Cybersecurity Framework (CSF) is a specific risk and cybersecurity framework. It provides guidance on controls that can be implemented to help improve an organisation’s cybersecurity risk posture. The CSF Functions consist of Identify, Protect, Detect, Respond, and Recover. Like most Information Technology (IT) frameworks, there are elements of people, processes, and technology. The same elements are required to successfully implement the NIST CSF. This research specifically focuses on the technology element. While there are many commercial technologies available for a small to medium sized business, the costs can be prohibitively expensive. Therefore, this research investigates cost-effective technologies and assesses their alignment to the NIST CSF. The assessment was made against the NIST CSF subcategories. Each subcategory was analysed to identify where a technology would likely be required. The framework provides a list of Informative References. These Informative References were used to create high- level technology categories, as well as identify the technical controls against which the technologies were measured. The technologies tested were either open source or proprietary. All open source technologies tested were free to use, or have a free community edition. Proprietary technologies would be free to use, or considered generally available to most organisations, such as components contained within Microsoft platforms. The results from the experimentation demonstrated that there are multiple cost-effective technologies that can support the NIST CSF. Once all technologies were tested, the NIST CSF was extended. Two new columns were added, namely high-level technology category, and tested technology. The columns were populated with output from the research. This extended framework begins an initial collection of cost-effective technologies in support of the NIST CSF.
- Full Text:
- Date Issued: 2018
Towards understanding the effect of size variation on the aggressive and feeding behaviours of juvenile dusky kob Argyrosomus japonicus (Pisces: Sciaenidae)
- Authors: Babane, Siviwe Elvis
- Date: 2018
- Subjects: Argyrosomus -- Feeding and feeds , Aquaculture , Fishes -- Cannibalism , Fishes -- Feeding and feeds
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/59592 , vital:27629
- Description: Many studies have been conducted on the effect of size-grading in other fish species. However, there is a paucity of scientific information on the effects of size variation on cannibalism of juvenile dusky kob. Thus, a study focusing on the effect of size variation on juvenile dusky kob aggressive and feeding (browsing) behaviours was conducted. Three separate groups of hatchery-reared juvenile dusky kob of were obtained from Oceanwise (Pty) Ltd for use in a series of three experimental trials. In all trials, juvenile fish of the same age were size-graded and the COV-value was used to determine the size variation. The focal fish (largest fish) was exposed to groups of fish with different size variation for 30-min. behavioural observations before and after feeding in randomised trials. The first experimental trial (Chapter 2) quantified the effect of increasing size variation and observation time on the aggressive and browsing behaviours of juvenile dusky kob. On average, juvenile dusky kob weighed 3.60 ± 0.68 g fish-1 and measured 5.8 ± 0.41 mm. Each focal fish was observed (a) before feeding in the morning, (b) 2 h after feeding, (c) 6 h after feeding and (d) 12 h after feeding. Fish increased browsing behaviours (averaging 6.60 ± 0.56) and decreased intimidating aggressive behaviours (18.60 ± 1.39) 12 h after feeding. Other aggressive behaviours occurred but did not differ between observation times. Aggressive and browsing behaviours positively correlated with size variation variables, predominantly, 12 h after feeding. An average frequency of 19 chases were observed positively correlated with size variation, followed by average frequencies of 17 body bites and browses, and 11 tail bites per 30 min. Some behaviours including average frequencies of 0.2 chases, 4 tail bites, 2.4 intimidating and 0.3 browsing behaviours negatively correlated with the size variation, generally closer to the last meal. These preliminary observations thus showed that fish have become hungry approximately 6-12 h after feeding and substituted certain behaviours for others as time after feeding passed and as size variation increased. The second experimental trial (Chapter 3) consisted of the observations further testing the relationship between aggressive behaviours and size variation of juveniles of dusky kob averaging 0.43 ± 0.27 g fish-1. The focal fish was exposed to groups of fish of four size variation (COV) treatments for observations before and 12 h after feeding. Aggressive behaviours positively correlated with size variation both before and 12 h after feeding. An average frequency of 437 body bites positively correlated more often with COV, followed by average frequencies of 365 intimidating behaviours and 199 tail bites per 30 min., respectively, before and 12 h after feeding. The least often exhibited aggressive behaviours averaged 26 chases while positively correlating with size variation on the times specified. An average frequency of 311 intimidating behaviours before and after feeding including average of 28 tail bites after feeding negatively correlated with size variation. This may relate to shift of behaviours depending on the needs and capacity of the fish. Apparently, aggressive fish can change its behaviour as a function of COV-values rather than the mean size of the other fish. The third trial (Chapter 4) investigated the effect of the aggressor’s (focal fish) size in relation its aggressive and browsing behaviours to other fish (non-focal fish). Juvenile fish used for this trial, on average, weighed 30 ± 7.63 g fish-1. Aggressive and browsing behaviours were observed in four treatments of a) high COV and mean weight below, b) low COV and mean weight less, c) high COV and mean weight equivalent to and d) low COV and mean weight higher than that of the focal fish. Increased frequencies of aggressive and browsing behaviours per 30 min. occurred in treatment A, sharing similar frequencies in treatment C, compared to the other treatments (B and D) which shared certain frequencies. The intimidating behaviours predominated, followed by browsing, body bites, chases and tail bites, respectively. The results of the overall study suggest that the time passed after feeding and increasing size variation and differences facilitated aggressive and browsing behaviours in juvenile dusky kob. Dusky kob showed increasing aggressive behaviours as early as in the first two weeks after hatching, averaging 0.43 ± 0.27 g fish-1 with the frequency correlating with size differences. Consistent size-grading technique in the same-age fish should be used to manage size variation associated with aggressive behaviours. The period of about 4-6 h after feeding may explain the noticeable increased aggressive and browsing behaviours. Thus, fish should be fed immediately before or after evacuation of their guts to maintain less-aggressive behaviours of juvenile dusky kob. Fish generally increased aggressive and browsing acts before and long time after feeding than closer to after feeding. The study has provided the fundamental scientific groundwork for fish farmers and future researchers can further explore size variation, time after feeding and gut evacuation rate as critical components of aggressive behaviours. The scientific knowledge of aggressive and cannibalistic behaviours has essential application in farming management to achieve improved survival and growth rates in juvenile fish.
- Full Text:
- Date Issued: 2018
- Authors: Babane, Siviwe Elvis
- Date: 2018
- Subjects: Argyrosomus -- Feeding and feeds , Aquaculture , Fishes -- Cannibalism , Fishes -- Feeding and feeds
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/59592 , vital:27629
- Description: Many studies have been conducted on the effect of size-grading in other fish species. However, there is a paucity of scientific information on the effects of size variation on cannibalism of juvenile dusky kob. Thus, a study focusing on the effect of size variation on juvenile dusky kob aggressive and feeding (browsing) behaviours was conducted. Three separate groups of hatchery-reared juvenile dusky kob of were obtained from Oceanwise (Pty) Ltd for use in a series of three experimental trials. In all trials, juvenile fish of the same age were size-graded and the COV-value was used to determine the size variation. The focal fish (largest fish) was exposed to groups of fish with different size variation for 30-min. behavioural observations before and after feeding in randomised trials. The first experimental trial (Chapter 2) quantified the effect of increasing size variation and observation time on the aggressive and browsing behaviours of juvenile dusky kob. On average, juvenile dusky kob weighed 3.60 ± 0.68 g fish-1 and measured 5.8 ± 0.41 mm. Each focal fish was observed (a) before feeding in the morning, (b) 2 h after feeding, (c) 6 h after feeding and (d) 12 h after feeding. Fish increased browsing behaviours (averaging 6.60 ± 0.56) and decreased intimidating aggressive behaviours (18.60 ± 1.39) 12 h after feeding. Other aggressive behaviours occurred but did not differ between observation times. Aggressive and browsing behaviours positively correlated with size variation variables, predominantly, 12 h after feeding. An average frequency of 19 chases were observed positively correlated with size variation, followed by average frequencies of 17 body bites and browses, and 11 tail bites per 30 min. Some behaviours including average frequencies of 0.2 chases, 4 tail bites, 2.4 intimidating and 0.3 browsing behaviours negatively correlated with the size variation, generally closer to the last meal. These preliminary observations thus showed that fish have become hungry approximately 6-12 h after feeding and substituted certain behaviours for others as time after feeding passed and as size variation increased. The second experimental trial (Chapter 3) consisted of the observations further testing the relationship between aggressive behaviours and size variation of juveniles of dusky kob averaging 0.43 ± 0.27 g fish-1. The focal fish was exposed to groups of fish of four size variation (COV) treatments for observations before and 12 h after feeding. Aggressive behaviours positively correlated with size variation both before and 12 h after feeding. An average frequency of 437 body bites positively correlated more often with COV, followed by average frequencies of 365 intimidating behaviours and 199 tail bites per 30 min., respectively, before and 12 h after feeding. The least often exhibited aggressive behaviours averaged 26 chases while positively correlating with size variation on the times specified. An average frequency of 311 intimidating behaviours before and after feeding including average of 28 tail bites after feeding negatively correlated with size variation. This may relate to shift of behaviours depending on the needs and capacity of the fish. Apparently, aggressive fish can change its behaviour as a function of COV-values rather than the mean size of the other fish. The third trial (Chapter 4) investigated the effect of the aggressor’s (focal fish) size in relation its aggressive and browsing behaviours to other fish (non-focal fish). Juvenile fish used for this trial, on average, weighed 30 ± 7.63 g fish-1. Aggressive and browsing behaviours were observed in four treatments of a) high COV and mean weight below, b) low COV and mean weight less, c) high COV and mean weight equivalent to and d) low COV and mean weight higher than that of the focal fish. Increased frequencies of aggressive and browsing behaviours per 30 min. occurred in treatment A, sharing similar frequencies in treatment C, compared to the other treatments (B and D) which shared certain frequencies. The intimidating behaviours predominated, followed by browsing, body bites, chases and tail bites, respectively. The results of the overall study suggest that the time passed after feeding and increasing size variation and differences facilitated aggressive and browsing behaviours in juvenile dusky kob. Dusky kob showed increasing aggressive behaviours as early as in the first two weeks after hatching, averaging 0.43 ± 0.27 g fish-1 with the frequency correlating with size differences. Consistent size-grading technique in the same-age fish should be used to manage size variation associated with aggressive behaviours. The period of about 4-6 h after feeding may explain the noticeable increased aggressive and browsing behaviours. Thus, fish should be fed immediately before or after evacuation of their guts to maintain less-aggressive behaviours of juvenile dusky kob. Fish generally increased aggressive and browsing acts before and long time after feeding than closer to after feeding. The study has provided the fundamental scientific groundwork for fish farmers and future researchers can further explore size variation, time after feeding and gut evacuation rate as critical components of aggressive behaviours. The scientific knowledge of aggressive and cannibalistic behaviours has essential application in farming management to achieve improved survival and growth rates in juvenile fish.
- Full Text:
- Date Issued: 2018
Two-tissue stable isotope analysis to elucidate isotopic incorporation and trophic niche patterns for chubbyhead barb Enteromius anoplus
- Authors: Kambikambi, Manda Juliet
- Date: 2018
- Subjects: Food chains (Ecology) , Barbus -- South Africa -- Great Fish River Estuary , Stable isotopes , Freshwater fishes -- Feeding and feeds , Freshwater fishes -- Food , Fins (Anatomy) , Akaike Information Criterion , Freshwater fishes -- Conservation , Chubbyhead barb Enteromius anoplus
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/61906 , vital:28082
- Description: Knowledge of trophic ecology underpins conservation and management of threatened species. Stable isotope analysis has been widely used as a more objective approach for elucidating the trophic positions of freshwater fishes. Until recently, stable isotope analysis for trophic ecology studies in freshwater fishes largely utilised white muscle tissue. This sampling approach, however, involves either euthanasia or muscle biopsy procedures that may be inappropriate for small-sized and endangered fishes. These concerns raised the need to explore and validate the utility of non-lethal alternatives such as fin clips, mucus and scales. The present study investigated the use of caudal fin tissue as a potential non-lethal alternative to muscle tissue for trophic studies on the chubbyhead barb Enteromius anoplus. The chubbyhead barb was selected as a model taxon for the present study because it is closely related or comparable in body size to a number of highly threatened small-bodied minnows in southern Africa. The chubbyhead barb was also considered an ideal species for this study because it is widespread, abundant and classified as Least Concern on the IUCN list of threatened species. The study used a two-pronged approach based on laboratory and field experiments. A laboratory experiment was conducted to quantify isotopic turnover rates and diet-tissue discrimination factors (DTDFs/A) for both muscle and fin tissues. This involved feeding chubbyhead barb two diets with distinct carbon (δ13C) and nitrogen (δ15N) values, and monitoring the temporal isotopic incorporation patterns into the two tissues. These patterns were assessed by applying least squares non-linear one- and two-compartment isotopic kinetics models. Model comparisons, based on Akaike information criterion (AIC), revealed that one- compartment models described isotopic incorporation patterns better than two-compartment models for both muscle and fin tissues. For δ13C, relatively short and comparable turnover rates were observed for muscle and fin tissues, which suggests that fin tissue could potentially provide similar inference as muscle tissue when assessing short term dietary patterns for chubbyhead barb. In contrast to δ13C, turnover rates for δ15N between muscle and fin tissue were different for both diets. Specifically, stable isotope incorporation turnover rate was faster in muscle tissue for animals that were fed on isotopically enriched diets compared to fin tissue. Conversely, stable isotope incorporation into fin tissue was faster in animals fed on isotopically depleted diets compared to muscle tissue. This suggests that knowledge of animal diet is critical when inferring fin tissue δ15N turnover rates, particularly when extrapolating both short and long term dietary patterns. Diet-tissue discrimination factors were influenced by diet type, with the fish fed on isotopically enriched diet having lower DTDFs than animals fed on isotopically depleted diets. This variation may be explained by the protein quality hypothesis, which suggests that the DTDFs of consumers will decrease as protein quality increases. When A13C and A15N values were averaged across diets in muscle and fin tissue, the values were 0.74‰ and 0.64‰, respectively, for A13C, and 5.53‰ and 5.83 ‰, respectively, for A15N. This appeared to be consistent with studies on other taxa for A13C (0-1 ‰), but for A15N (3-5 ‰) the results of this study were higher than those reported for other taxa. These results suggest that investigating appropriate DTDFs for both muscle and fin tissues is important in trophic ecology studies of these minnows. A field-based study was conducted to investigate temporal dynamics in food web patterns for chubbyhead barb in the wild within the headwaters of the Koonap River, a tributary of the Great Fish River, in the Eastern Cape, South Africa. This was achieved by collecting and comparing stable isotope data for chubbyhead barb and its potential food sources on a seasonal scale. There was a discernible difference in both the composition of carbon and nitrogen isotope values for basal food sources and macroinvertebrate communities, which suggests that this headwater stream was subject to temporal changes in food web dynamics. For chubbyhead barb, comparison of its isotopic niche sizes on a temporal scale based on both muscle and fin tissue showed differences across seasons. Furthermore, isotopic niche sizes inferred from fin tissue were larger than those inferred from muscle tissue during winter and spring, whereas during summer and autumn the isotopic niche sizes inferred from muscle and fin tissue were generally comparable. This suggests the likely influence of different metabolic and physiological processes that these two tissues undergo on a temporal scale. Therefore, difference in tissue type, and their associated metabolic pathways should be considered when using fin tissue as a substitute for muscle tissue on broad temporal scales. The results from this study indicated that caudal fin tissue has the potential to be a substitute for muscle in trophic studies of chubbyhead barb Enteromius anoplus, as well as other related small bodied endangered minnow species from South Africa.
- Full Text:
- Date Issued: 2018
- Authors: Kambikambi, Manda Juliet
- Date: 2018
- Subjects: Food chains (Ecology) , Barbus -- South Africa -- Great Fish River Estuary , Stable isotopes , Freshwater fishes -- Feeding and feeds , Freshwater fishes -- Food , Fins (Anatomy) , Akaike Information Criterion , Freshwater fishes -- Conservation , Chubbyhead barb Enteromius anoplus
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/61906 , vital:28082
- Description: Knowledge of trophic ecology underpins conservation and management of threatened species. Stable isotope analysis has been widely used as a more objective approach for elucidating the trophic positions of freshwater fishes. Until recently, stable isotope analysis for trophic ecology studies in freshwater fishes largely utilised white muscle tissue. This sampling approach, however, involves either euthanasia or muscle biopsy procedures that may be inappropriate for small-sized and endangered fishes. These concerns raised the need to explore and validate the utility of non-lethal alternatives such as fin clips, mucus and scales. The present study investigated the use of caudal fin tissue as a potential non-lethal alternative to muscle tissue for trophic studies on the chubbyhead barb Enteromius anoplus. The chubbyhead barb was selected as a model taxon for the present study because it is closely related or comparable in body size to a number of highly threatened small-bodied minnows in southern Africa. The chubbyhead barb was also considered an ideal species for this study because it is widespread, abundant and classified as Least Concern on the IUCN list of threatened species. The study used a two-pronged approach based on laboratory and field experiments. A laboratory experiment was conducted to quantify isotopic turnover rates and diet-tissue discrimination factors (DTDFs/A) for both muscle and fin tissues. This involved feeding chubbyhead barb two diets with distinct carbon (δ13C) and nitrogen (δ15N) values, and monitoring the temporal isotopic incorporation patterns into the two tissues. These patterns were assessed by applying least squares non-linear one- and two-compartment isotopic kinetics models. Model comparisons, based on Akaike information criterion (AIC), revealed that one- compartment models described isotopic incorporation patterns better than two-compartment models for both muscle and fin tissues. For δ13C, relatively short and comparable turnover rates were observed for muscle and fin tissues, which suggests that fin tissue could potentially provide similar inference as muscle tissue when assessing short term dietary patterns for chubbyhead barb. In contrast to δ13C, turnover rates for δ15N between muscle and fin tissue were different for both diets. Specifically, stable isotope incorporation turnover rate was faster in muscle tissue for animals that were fed on isotopically enriched diets compared to fin tissue. Conversely, stable isotope incorporation into fin tissue was faster in animals fed on isotopically depleted diets compared to muscle tissue. This suggests that knowledge of animal diet is critical when inferring fin tissue δ15N turnover rates, particularly when extrapolating both short and long term dietary patterns. Diet-tissue discrimination factors were influenced by diet type, with the fish fed on isotopically enriched diet having lower DTDFs than animals fed on isotopically depleted diets. This variation may be explained by the protein quality hypothesis, which suggests that the DTDFs of consumers will decrease as protein quality increases. When A13C and A15N values were averaged across diets in muscle and fin tissue, the values were 0.74‰ and 0.64‰, respectively, for A13C, and 5.53‰ and 5.83 ‰, respectively, for A15N. This appeared to be consistent with studies on other taxa for A13C (0-1 ‰), but for A15N (3-5 ‰) the results of this study were higher than those reported for other taxa. These results suggest that investigating appropriate DTDFs for both muscle and fin tissues is important in trophic ecology studies of these minnows. A field-based study was conducted to investigate temporal dynamics in food web patterns for chubbyhead barb in the wild within the headwaters of the Koonap River, a tributary of the Great Fish River, in the Eastern Cape, South Africa. This was achieved by collecting and comparing stable isotope data for chubbyhead barb and its potential food sources on a seasonal scale. There was a discernible difference in both the composition of carbon and nitrogen isotope values for basal food sources and macroinvertebrate communities, which suggests that this headwater stream was subject to temporal changes in food web dynamics. For chubbyhead barb, comparison of its isotopic niche sizes on a temporal scale based on both muscle and fin tissue showed differences across seasons. Furthermore, isotopic niche sizes inferred from fin tissue were larger than those inferred from muscle tissue during winter and spring, whereas during summer and autumn the isotopic niche sizes inferred from muscle and fin tissue were generally comparable. This suggests the likely influence of different metabolic and physiological processes that these two tissues undergo on a temporal scale. Therefore, difference in tissue type, and their associated metabolic pathways should be considered when using fin tissue as a substitute for muscle tissue on broad temporal scales. The results from this study indicated that caudal fin tissue has the potential to be a substitute for muscle in trophic studies of chubbyhead barb Enteromius anoplus, as well as other related small bodied endangered minnow species from South Africa.
- Full Text:
- Date Issued: 2018
Understanding and quantifying channel transmission loss processes in the Limpopo River Basin
- Authors: Mvandaba, Vuyelwa
- Date: 2018
- Subjects: Water-supply -- Management , Water-supply -- Limpopo River Watershed , Alluvium -- Limpopo River Watershed , Streamflow -- Limpopo River Watershed
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63797 , vital:28490
- Description: Water availability is one of the major societal issues facing the world. The ability to understand and quantify the impact of key hydrological processes on the availability of water resources is therefore integral to ensuring equitable and sustainable resource management. A review of previous hydrological studies conducted in the Limpopo River Basin has revealed a gap in the understanding of surface water-groundwater interactions, particularly channel transmission loss processes. These earlier studies, focused largely on the Limpopo River’s main stem, have attributed the existence of these streamflow losses to the presence of significant alluvial aquifers and indicated that the losses account for about 30 percent (or 1000 Mm3 a-1) of the basin’s water balance. The work conducted in this dissertation reports on the delineation of alluvial aquifers across three sub-basins of the Limpopo River Basin namely, the Mokolo (South Africa), Motloutse (Botswana) and Mzingwane (Zimbabwe) sub-basins and the estimation of potential channel transmission losses based on the alluvial aquifer properties. Additionally, an assessment of the different approaches that can be applied to simulate these channel transmission losses in the Pitman Model is presented. To delineate alluvial aquifers, general land cover classes including alluvial aquifers were produced from Landsat-8 imagery through image classification. The areal extent of the delineated alluvial aquifers was calculated using ArcMap 10.3. To quantify channel transmission losses and determine the effects on regional water resources, three approaches using the Pitman model were applied. The three approaches include an explicit transmission loss function, the use of a wetland function to represent channel-floodplain storage exchanges and the use of a ‘dummy’ reservoir to represent floodplain storage and evapotranspiration losses. Results indicate that all three approaches were able to simulate channel transmission losses, although with differing magnitudes. Observed monthly flow data were used to as a means of validating loss simulations however for each sub-basin, medium and low flows were over-simulated which accounts for water uses that were inefficiently represented due to lack of data. Knowledge of the structure of the transmission loss function dictates that it is better at representing the dynamics of channel transmission losses, as it takes into account the contribution of losses to groundwater recharge whereas the other two functions simply store water and release it back to the channel. Overall, the hydrological modelling results demonstrate the potential of each approach in reproducing the dynamics of channel transmission losses between channel and alluvial aquifer within an existing sub-basin scale hydrological model. It is believed that better quantification of losses and more efficient qualitative determination of the function which best represents transmission losses, can be attained with more reliable observed data. In conclusion, a study of this nature can be beneficial to water resource estimation programmes as it highlights the uncertainties related with quantifying channel transmission loss processes in a semi-arid environment.
- Full Text:
- Date Issued: 2018
- Authors: Mvandaba, Vuyelwa
- Date: 2018
- Subjects: Water-supply -- Management , Water-supply -- Limpopo River Watershed , Alluvium -- Limpopo River Watershed , Streamflow -- Limpopo River Watershed
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63797 , vital:28490
- Description: Water availability is one of the major societal issues facing the world. The ability to understand and quantify the impact of key hydrological processes on the availability of water resources is therefore integral to ensuring equitable and sustainable resource management. A review of previous hydrological studies conducted in the Limpopo River Basin has revealed a gap in the understanding of surface water-groundwater interactions, particularly channel transmission loss processes. These earlier studies, focused largely on the Limpopo River’s main stem, have attributed the existence of these streamflow losses to the presence of significant alluvial aquifers and indicated that the losses account for about 30 percent (or 1000 Mm3 a-1) of the basin’s water balance. The work conducted in this dissertation reports on the delineation of alluvial aquifers across three sub-basins of the Limpopo River Basin namely, the Mokolo (South Africa), Motloutse (Botswana) and Mzingwane (Zimbabwe) sub-basins and the estimation of potential channel transmission losses based on the alluvial aquifer properties. Additionally, an assessment of the different approaches that can be applied to simulate these channel transmission losses in the Pitman Model is presented. To delineate alluvial aquifers, general land cover classes including alluvial aquifers were produced from Landsat-8 imagery through image classification. The areal extent of the delineated alluvial aquifers was calculated using ArcMap 10.3. To quantify channel transmission losses and determine the effects on regional water resources, three approaches using the Pitman model were applied. The three approaches include an explicit transmission loss function, the use of a wetland function to represent channel-floodplain storage exchanges and the use of a ‘dummy’ reservoir to represent floodplain storage and evapotranspiration losses. Results indicate that all three approaches were able to simulate channel transmission losses, although with differing magnitudes. Observed monthly flow data were used to as a means of validating loss simulations however for each sub-basin, medium and low flows were over-simulated which accounts for water uses that were inefficiently represented due to lack of data. Knowledge of the structure of the transmission loss function dictates that it is better at representing the dynamics of channel transmission losses, as it takes into account the contribution of losses to groundwater recharge whereas the other two functions simply store water and release it back to the channel. Overall, the hydrological modelling results demonstrate the potential of each approach in reproducing the dynamics of channel transmission losses between channel and alluvial aquifer within an existing sub-basin scale hydrological model. It is believed that better quantification of losses and more efficient qualitative determination of the function which best represents transmission losses, can be attained with more reliable observed data. In conclusion, a study of this nature can be beneficial to water resource estimation programmes as it highlights the uncertainties related with quantifying channel transmission loss processes in a semi-arid environment.
- Full Text:
- Date Issued: 2018
Understanding the extension capacity needs of the CapeNature Stewardship Programme in the Western Cape Province of South Africa
- Coetzee, Johannes Christiaan
- Authors: Coetzee, Johannes Christiaan
- Date: 2018
- Subjects: Conservation projects (Natural resources) , Psychometrics , Adaptive natural resource management , Biodiversity conservation , CapeNature Stewardship Programme
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63220 , vital:28383
- Description: There is an increasing call for conservation programmes to provide sound evidence of effectiveness, and employing empirical evaluations can assist in the transition to evidence-based conservation practices. The objectives of this research were to develop a logic model for the CapeNature Stewardship Programme which would articulate the programme's theory of operation with respect to its Stewardship Programme landholders. The second major objective was to develop psychometric instruments for assessing the motivations and satisfactions of the programme's stewardship landholders. Both objectives included the aim to provide robust and repeatable instruments for exploring landholder's psychology, and developing a programme's theory of operation to understand the programme and improve with understanding the needs of the landowners. In this regard the processes and methodologies employed represent a major component of this research. A mixed methods approach was utilized, including stakeholder and volunteer surveys, conducted via mailing hardcopies and the internet, together with three focus groups held with the programme's management, extension staff and the stewardship landholders. Analysis of the data thus collected included both qualitative and quantitative approaches, specifically coding and content analysis, together with statistical tests of internal consistency, factor analysis and doubling correspondence analysis. Robust indices for example validity and internal consistency were developed for assessing landholder’s satisfaction with extension and level of satisfaction with the stewardship programme (Babbie 2007). These indices revealed that landholders in the Stewardship Programme are not satisfied with the programme, and exhibit behaviours suggesting they act as advocates for the programme. Demographic data and additional information provided further insights into the programme. The development of a method for articulating the programme's theory of operation is represented, together with four logic models which graphically illustrate this theory. This process and theory allowed for recommendations to be provided for the programme's improvement. A platform for adaptive management and further evaluations of this, and similar programmes, represents a major outcome of this research, understanding the extension capacity needs for the conservation of biodiversity in the CapeNature Stewardship Programme to function as a model for improving the implementation of the programme across the Western Cape, South Africa. This research feeds into an evaluation of CapeNature’s Biodiveristy Stewardship programme and demonstrates the importance of incorporating psychology into conservation interventions.
- Full Text:
- Date Issued: 2018
- Authors: Coetzee, Johannes Christiaan
- Date: 2018
- Subjects: Conservation projects (Natural resources) , Psychometrics , Adaptive natural resource management , Biodiversity conservation , CapeNature Stewardship Programme
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63220 , vital:28383
- Description: There is an increasing call for conservation programmes to provide sound evidence of effectiveness, and employing empirical evaluations can assist in the transition to evidence-based conservation practices. The objectives of this research were to develop a logic model for the CapeNature Stewardship Programme which would articulate the programme's theory of operation with respect to its Stewardship Programme landholders. The second major objective was to develop psychometric instruments for assessing the motivations and satisfactions of the programme's stewardship landholders. Both objectives included the aim to provide robust and repeatable instruments for exploring landholder's psychology, and developing a programme's theory of operation to understand the programme and improve with understanding the needs of the landowners. In this regard the processes and methodologies employed represent a major component of this research. A mixed methods approach was utilized, including stakeholder and volunteer surveys, conducted via mailing hardcopies and the internet, together with three focus groups held with the programme's management, extension staff and the stewardship landholders. Analysis of the data thus collected included both qualitative and quantitative approaches, specifically coding and content analysis, together with statistical tests of internal consistency, factor analysis and doubling correspondence analysis. Robust indices for example validity and internal consistency were developed for assessing landholder’s satisfaction with extension and level of satisfaction with the stewardship programme (Babbie 2007). These indices revealed that landholders in the Stewardship Programme are not satisfied with the programme, and exhibit behaviours suggesting they act as advocates for the programme. Demographic data and additional information provided further insights into the programme. The development of a method for articulating the programme's theory of operation is represented, together with four logic models which graphically illustrate this theory. This process and theory allowed for recommendations to be provided for the programme's improvement. A platform for adaptive management and further evaluations of this, and similar programmes, represents a major outcome of this research, understanding the extension capacity needs for the conservation of biodiversity in the CapeNature Stewardship Programme to function as a model for improving the implementation of the programme across the Western Cape, South Africa. This research feeds into an evaluation of CapeNature’s Biodiveristy Stewardship programme and demonstrates the importance of incorporating psychology into conservation interventions.
- Full Text:
- Date Issued: 2018
Using action cameras to assess habitat use by Pseudobarbus afer and Sandelia capensis in the Swartkops River, Eastern Cape, South Africa
- Authors: Hannweg, Bianca
- Date: 2018
- Subjects: Freshwater biodiversity -- South Africa -- Eastern Cape , Freshwater biodiversity conservation -- South Africa -- Eastern Cape , Freshwater ecology -- South Africa -- Eastern Cape , Freshwater fishes -- South Africa -- Eastern Cape , Freshwater fishes -- Behavior -- South Africa -- Eastern Cape , Freshwater fishes -- Habitat -- South Africa -- Eastern Cape , Underwater videography , Anabantidae -- South Africa -- Eastern Cape , Cyprinidae -- South Africa -- Eastern Cape , Pseudobarbus afer , Sandelia capensis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62039 , vital:28098
- Description: Currently, freshwater systems are facing various threats, freshwater biota are declining and there is an increased need to monitor freshwater fauna and flora using non-invasive methods. This thesis aimed to evaluate the potential of using action cameras as a tool for the monitoring of freshwater fish populations and the collection of habitat utilisation data. This evaluation was conducted in headwater tributaries of the Swartkops River in South Africa, using two threatened species that have not been extensively studied, Pseudobarbus afer (Peters, 1864) and Sandelia capensis (Cuvier, 1831). The aims of this study were to: (1) assess the use of underwater video analysis (UWVA) using action cameras (videos and still photographs) in comparison to estimates derived from snorkel surveys, to estimate the abundance of P. afer in headwater streams; (2) use estimates derived from UWVA (videos and photographs) to assess the habitat use of two imperilled species, P. afer and S. capensis; and (3) assess habitat use by these two species in the presence of an artificial habitat. This work demonstrated that: (1) estimates derived from videos and photographs were strongly correlated to estimates derived from snorkel surveys, provided multiple cameras were used; (2) estimates derived from videos and photographs were not significantly different to estimates derived from snorkel surveys; (3) a filming period of 15 minutes was sufficient at detecting 0.9 of the cumulative mMaxN (mean MaxN) within one of the five habitats; and (4) still photographs, which are less time consuming to process than videos, could be used in preference to videos. Based on these findings, techniques using action cameras to assess habitat utilisation and behaviour in situ of the two-focal species, were developed using six habitat types (inflow, outflow, woody debris, fern root, middle and artificial) in four pools. It was demonstrated that: (1) there was a significant difference in proportional occupancies across habitats for both P. afer and S. capensis; (2) Pseudobarbus afer were mainly observed schooling in the middle of the pool and feeding on detritus material in fern root, woody debris and off the artificial structure; and (3) Sandelia capensis rapidly colonised the artificial structure and were observed utilising it for refuge. In conclusion, this work demonstrated that still photographs from action cameras can be used in place of videos to estimate the abundance of freshwater fishes and assess their habitat use and behaviour in clear headwater streams. This work also demonstrated how action cameras could be used to evaluate the effect of the introduction of artificial habitat as a restoration measure for headwater fish communities.
- Full Text:
- Date Issued: 2018
- Authors: Hannweg, Bianca
- Date: 2018
- Subjects: Freshwater biodiversity -- South Africa -- Eastern Cape , Freshwater biodiversity conservation -- South Africa -- Eastern Cape , Freshwater ecology -- South Africa -- Eastern Cape , Freshwater fishes -- South Africa -- Eastern Cape , Freshwater fishes -- Behavior -- South Africa -- Eastern Cape , Freshwater fishes -- Habitat -- South Africa -- Eastern Cape , Underwater videography , Anabantidae -- South Africa -- Eastern Cape , Cyprinidae -- South Africa -- Eastern Cape , Pseudobarbus afer , Sandelia capensis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62039 , vital:28098
- Description: Currently, freshwater systems are facing various threats, freshwater biota are declining and there is an increased need to monitor freshwater fauna and flora using non-invasive methods. This thesis aimed to evaluate the potential of using action cameras as a tool for the monitoring of freshwater fish populations and the collection of habitat utilisation data. This evaluation was conducted in headwater tributaries of the Swartkops River in South Africa, using two threatened species that have not been extensively studied, Pseudobarbus afer (Peters, 1864) and Sandelia capensis (Cuvier, 1831). The aims of this study were to: (1) assess the use of underwater video analysis (UWVA) using action cameras (videos and still photographs) in comparison to estimates derived from snorkel surveys, to estimate the abundance of P. afer in headwater streams; (2) use estimates derived from UWVA (videos and photographs) to assess the habitat use of two imperilled species, P. afer and S. capensis; and (3) assess habitat use by these two species in the presence of an artificial habitat. This work demonstrated that: (1) estimates derived from videos and photographs were strongly correlated to estimates derived from snorkel surveys, provided multiple cameras were used; (2) estimates derived from videos and photographs were not significantly different to estimates derived from snorkel surveys; (3) a filming period of 15 minutes was sufficient at detecting 0.9 of the cumulative mMaxN (mean MaxN) within one of the five habitats; and (4) still photographs, which are less time consuming to process than videos, could be used in preference to videos. Based on these findings, techniques using action cameras to assess habitat utilisation and behaviour in situ of the two-focal species, were developed using six habitat types (inflow, outflow, woody debris, fern root, middle and artificial) in four pools. It was demonstrated that: (1) there was a significant difference in proportional occupancies across habitats for both P. afer and S. capensis; (2) Pseudobarbus afer were mainly observed schooling in the middle of the pool and feeding on detritus material in fern root, woody debris and off the artificial structure; and (3) Sandelia capensis rapidly colonised the artificial structure and were observed utilising it for refuge. In conclusion, this work demonstrated that still photographs from action cameras can be used in place of videos to estimate the abundance of freshwater fishes and assess their habitat use and behaviour in clear headwater streams. This work also demonstrated how action cameras could be used to evaluate the effect of the introduction of artificial habitat as a restoration measure for headwater fish communities.
- Full Text:
- Date Issued: 2018
Water quality, biomass and extracellular polymeric substances in an integrated algae pond system
- Authors: Jimoh, Taobat Adekilekun
- Date: 2018
- Subjects: Water -- Purification , Sewage -- Purification -- Anaerobic treatment , Sewage lagoons , Sewage disposal plants , ASPAM model (Acid mine drainage) , Integrated algae pond systems (IAPS)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/57307 , vital:26871
- Description: Integrated algae pond systems (IAPS) combine the use of anaerobic and aerobic bioprocesses to effect wastewater treatment. Although, IAPS as a technology process offers many advantages including efficient and simultaneous N and P removal, no requirement for additional chemicals, O2 generation, CO2 mitigation, and a biomass with potential for valorization, a lack of technological advancement and the need for large land area, has limited the reach of this technology at industrial scale. In mitigation, peroxonation was introduced as a tertiary treatment unit and its effect on COD and TSS of IAPS treated water investigated. An effort was made to characterize the soluble but persistent COD in IAPS treated water and, productivity of the HRAOP mixed liquor was investigated to gain insight into the potential use of this biomass. Results show that peroxone treatment effectively reduced COD, TSS, and nutrient load of IAPS water without any significant impact on land area requirement. Indeed, summary data describing the effect of peroxone on quality of IAPS-treated water confirmed that it complies with the general limit values for either irrigation or discharge into a water resource that is not a listed water resource for volumes up to 2 ML of treated wastewater on any given day. Extraction followed by FT-IR spectroscopy was used to confirm albeit tentatively, the identity of the soluble but persistent COD in IAPS treated water as MaB-floc EPS. Results show that MaB-flocs from HRAOPs are assemblages of microorganisms produced as discrete aggregates as a result of microbial EPS production. A relationship between photosynthesis and EPS production was established by quantification of the EPS following exposure of MaB-flocs to either continuous light or darkness. Several novel strains of bacteria were isolated from HRAOP mixed liquor and 16S ribosomal genomic sequence analysis resulted in the molecular characterization of Planococcus maitriensis strain ECCN 45b. This is the first report of Planococcus maitriensis from a wastewater treatment process. Productivity and change in MaB-flocs concentration, measured as mixed liquor suspended solids (MLSS) between morning and evening were monitored and revealed that MLSS is composed of microalgae and bacteria but not fungi. Concentration varied from 77 mg L-1 in September (winter) to 285 mg L-1 in November (spring); pond productivity increased from 5.8 g m-2 d-1 (winter) to 21.5 g m-2 d-1 (spring); and, irrespective of MLSS concentration in late afternoon, approximately 39% was lost overnight, which presumably occurred due to passive removal by the algae settling pond. The outcomes of this research are discussed in terms of the quality of treated water, and the further development of IAPS as a platform technology for establishing a biorefinery within the wastewater treatment sector.
- Full Text:
- Date Issued: 2018
- Authors: Jimoh, Taobat Adekilekun
- Date: 2018
- Subjects: Water -- Purification , Sewage -- Purification -- Anaerobic treatment , Sewage lagoons , Sewage disposal plants , ASPAM model (Acid mine drainage) , Integrated algae pond systems (IAPS)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/57307 , vital:26871
- Description: Integrated algae pond systems (IAPS) combine the use of anaerobic and aerobic bioprocesses to effect wastewater treatment. Although, IAPS as a technology process offers many advantages including efficient and simultaneous N and P removal, no requirement for additional chemicals, O2 generation, CO2 mitigation, and a biomass with potential for valorization, a lack of technological advancement and the need for large land area, has limited the reach of this technology at industrial scale. In mitigation, peroxonation was introduced as a tertiary treatment unit and its effect on COD and TSS of IAPS treated water investigated. An effort was made to characterize the soluble but persistent COD in IAPS treated water and, productivity of the HRAOP mixed liquor was investigated to gain insight into the potential use of this biomass. Results show that peroxone treatment effectively reduced COD, TSS, and nutrient load of IAPS water without any significant impact on land area requirement. Indeed, summary data describing the effect of peroxone on quality of IAPS-treated water confirmed that it complies with the general limit values for either irrigation or discharge into a water resource that is not a listed water resource for volumes up to 2 ML of treated wastewater on any given day. Extraction followed by FT-IR spectroscopy was used to confirm albeit tentatively, the identity of the soluble but persistent COD in IAPS treated water as MaB-floc EPS. Results show that MaB-flocs from HRAOPs are assemblages of microorganisms produced as discrete aggregates as a result of microbial EPS production. A relationship between photosynthesis and EPS production was established by quantification of the EPS following exposure of MaB-flocs to either continuous light or darkness. Several novel strains of bacteria were isolated from HRAOP mixed liquor and 16S ribosomal genomic sequence analysis resulted in the molecular characterization of Planococcus maitriensis strain ECCN 45b. This is the first report of Planococcus maitriensis from a wastewater treatment process. Productivity and change in MaB-flocs concentration, measured as mixed liquor suspended solids (MLSS) between morning and evening were monitored and revealed that MLSS is composed of microalgae and bacteria but not fungi. Concentration varied from 77 mg L-1 in September (winter) to 285 mg L-1 in November (spring); pond productivity increased from 5.8 g m-2 d-1 (winter) to 21.5 g m-2 d-1 (spring); and, irrespective of MLSS concentration in late afternoon, approximately 39% was lost overnight, which presumably occurred due to passive removal by the algae settling pond. The outcomes of this research are discussed in terms of the quality of treated water, and the further development of IAPS as a platform technology for establishing a biorefinery within the wastewater treatment sector.
- Full Text:
- Date Issued: 2018
An analysis of flood activity over the past century based on the sedimentary deposits in the Mfolozi floodplain
- Authors: Mbao, Chabala
- Date: 2017
- Subjects: Floodplain morphology -- South Africa -- Mfolozi River , Floodplains -- South Africa -- Kwazulu-Natal , Sedimentation and deposition -- South Africa -- Kwazulu-Natal , Floods -- South Africa -- Kwazulu-Natal -- Measurement , Hydrology -- Research -- South Africa -- Kwazulu-Natal , Stream measurements -- South Africa -- Kwazulu-Natal
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/53711 , vital:26312
- Description: Floods are natural phenomena that are of widespread interest to the scientific community, particularly in the context of understanding the impact of climate change as well as changing land use patterns and the security of infrastructure such as towns and roads. In northern KwaZulu-Natal, large flood events have been a reoccurring feature over the past century. The geographical position and extent of the region means that is affected by both inland and coastal weather phenomena, each with the potential to cause large flood events. While information pertaining to large floods in the region in the form of flow data is readily available, flow data is often incomplete and limited in terms of how far back in time the records extend. The Mfolozi River floodplain itself also houses a record of flood activity in the region in the form of sedimentary deposits, which have the potential to reveal flood activity over a much longer timescale, even when monitoring of flood activity in the region was not present. Establishing a link between the hydrological and sedimentary features of floods may be useful in establishing a record of flood activity extending beyond the limits of historical records. The aim of this project was to construct a record of major flood events on the Mfolozi River floodplain over the last century and determine their source. The first objective involved establishing the history of flood events in the Mfolozi River catchment utilising hydrological data recorded throughout the catchment. Thirty nine different large floods (defined as being over 800 m3.s-1) were recorded on the floodplain over the past century. The data also highlighted differences in the extent of known coastal and inland systems (tropical cyclones and cut-off lows respectively), allowing for inferences to be made about the area of the catchment most likely affected by each event; some flood events were identified as having mostly affected the upper reaches of the catchment, while others mostly affected the lower reaches, closer to the coast. The second objective was to identify the various physical, mineralogical and geochemical features of the sediment deposited on the Mfolozi floodplain. Multiple individual flood deposits were identified in the sedimentary record, with sediment tracing analysis providing insight into the source of the deposits within the catchment; no traces of igneous or metamorphic material could be found within the sediment. The source rocks were identified as mostly quartzite, with minor shale and sandstone deposits as well. This put the source of the sediment in the upper to middle reaches of the catchment. The sedimentary analysis was combined with the hydrological records to establish a chronology of flood events extending back to the 1960's. Unfortunately, this record could not be extended further due to the limited amount of sediment collected as well the limits of readily available analytical techniques; radionuclide dating methods were unsuccessful due to the low amounts of fallout radionuclides present in the sediment. The study has however successfully established a history of flood events in the region, as well as providing a link between the hydrological and sedimentary features of flood events that could potentially be useful for current and future research.
- Full Text:
- Date Issued: 2017
- Authors: Mbao, Chabala
- Date: 2017
- Subjects: Floodplain morphology -- South Africa -- Mfolozi River , Floodplains -- South Africa -- Kwazulu-Natal , Sedimentation and deposition -- South Africa -- Kwazulu-Natal , Floods -- South Africa -- Kwazulu-Natal -- Measurement , Hydrology -- Research -- South Africa -- Kwazulu-Natal , Stream measurements -- South Africa -- Kwazulu-Natal
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/53711 , vital:26312
- Description: Floods are natural phenomena that are of widespread interest to the scientific community, particularly in the context of understanding the impact of climate change as well as changing land use patterns and the security of infrastructure such as towns and roads. In northern KwaZulu-Natal, large flood events have been a reoccurring feature over the past century. The geographical position and extent of the region means that is affected by both inland and coastal weather phenomena, each with the potential to cause large flood events. While information pertaining to large floods in the region in the form of flow data is readily available, flow data is often incomplete and limited in terms of how far back in time the records extend. The Mfolozi River floodplain itself also houses a record of flood activity in the region in the form of sedimentary deposits, which have the potential to reveal flood activity over a much longer timescale, even when monitoring of flood activity in the region was not present. Establishing a link between the hydrological and sedimentary features of floods may be useful in establishing a record of flood activity extending beyond the limits of historical records. The aim of this project was to construct a record of major flood events on the Mfolozi River floodplain over the last century and determine their source. The first objective involved establishing the history of flood events in the Mfolozi River catchment utilising hydrological data recorded throughout the catchment. Thirty nine different large floods (defined as being over 800 m3.s-1) were recorded on the floodplain over the past century. The data also highlighted differences in the extent of known coastal and inland systems (tropical cyclones and cut-off lows respectively), allowing for inferences to be made about the area of the catchment most likely affected by each event; some flood events were identified as having mostly affected the upper reaches of the catchment, while others mostly affected the lower reaches, closer to the coast. The second objective was to identify the various physical, mineralogical and geochemical features of the sediment deposited on the Mfolozi floodplain. Multiple individual flood deposits were identified in the sedimentary record, with sediment tracing analysis providing insight into the source of the deposits within the catchment; no traces of igneous or metamorphic material could be found within the sediment. The source rocks were identified as mostly quartzite, with minor shale and sandstone deposits as well. This put the source of the sediment in the upper to middle reaches of the catchment. The sedimentary analysis was combined with the hydrological records to establish a chronology of flood events extending back to the 1960's. Unfortunately, this record could not be extended further due to the limited amount of sediment collected as well the limits of readily available analytical techniques; radionuclide dating methods were unsuccessful due to the low amounts of fallout radionuclides present in the sediment. The study has however successfully established a history of flood events in the region, as well as providing a link between the hydrological and sedimentary features of flood events that could potentially be useful for current and future research.
- Full Text:
- Date Issued: 2017
Chemical composition of leaf essential oils of Lantana camara varieties in South Africa and their effect on the behavioural preference of Falconia intermedia
- Authors: Ngxande-Koza, Samella W
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4877 , vital:20739
- Description: Lantana camara L. (Verbenaceae) is one the most problematic invaders in South Africa invading forest edges, sand dunes, and shorelines by forming impenetrable thickets. Lantana camara invasions degrade natural biodiversity, reduce the value of land and consequently it has been a target for biological control, over the last 50 years in South Africa. Studies that have reported on chemical profile of Lantana camara have been conducted around the world but not in South Africa. Hence, the first aim of the current study was to identify the chemical baseline of L. camara varieties in the Eastern Cape, South Africa. Recent studies have shown that feeding by one of the agents released against L. camara, Falconia intermedia (Distant) (Hemiptera: Miridae), induces anti-herbivory response through increased leaf toughness and trichome density. A preliminary study conducted also reported the production of volatile chemicals by one variety, Whitney Farm, due to feeding by the mirids. Therefore, the second aim was to determine the induced changes in chemical compounds of L. camara varieties after feeding by F. intermedia. A third aim was to determine the effect these chemical compounds have on the behaviour of F. intermedia. To identify the chemical baseline of L. camara varieties, the essential oils of four L. camara varieties (East London, Port Alfred, Whitney Farm and Heather Glen) were analysed using gas chromatography mass spectrometry (GC-MS) and that resulted to the identification of 163 constitutive and 75 induced chemicals across the varieties tested. Lantana camara varieties showed different chemical classes but were highly dominated by terpenes. A great variation in the number of constitutive chemical compounds was found in all the varieties. There were 56 constitutive chemical compounds in the Whitney Farm variety, 41 in the East London variety, 36 in the Heather Glen variety and 30 in the Port Alfred variety. The Whitney Farm variety had the highest number (22) of unique constitutive chemicals identified when compared with other varieties. This indicates the chemical distinctiveness of the Whitney Farm variety from the other varieties. In the varieties tested, there were common chemical compounds identified in constitutive and induced (discussed below) states of the plants such as caryophyllene, hexane, naphthalene, copaene and a-caryophyllene. Besides naphthalene, the majority of chemical compounds in South African L. camara varieties were similar to compounds that have been identified across the world, suggesting that they are closely related. The expression of naphthalene in these varieties may be due to changes in the chemicals expressed over evolutionary time as predicted by the Novel Weapons Hypothesis. Amongst the varieties, a great variation in chemical compounds and their concentrations was shown in the induced states of the plants. The concentration of constitutive caryophyllene ranged from (3.13 - 15.7) %, to (4.02 - 11.10) % after feeding. The concentration of constitutive hexane ranged from (6.13 - 71.19) %, to (33.3 - 75.8) % after feeding. The concentration of constitutive naphthalene ranged from (0.21 - 4.79) %, to (0.92 - 2.11) % after feeding. The concentration of constitutive copaene ranged from (0.57 - 1.57) %, to (1.20 - 2.72) %. Lastly, the concentration of constitutive a-caryophyllene ranged from (1.18 - 9.03) %, to (0.78 - 5.48) % after feeding. The changes in chemical concentrations in lantana varieties indicated that feeding by the mirid on L. camara varieties causes an induction by either reducing or increasing the chemical concentrations. To determine the effect of the identified compounds on the behaviour of F. intermedia adults, olfactometer bioassays were conducted using a Y-tube technique. A significantly higher proportion of F. intermedia were attracted to undamaged leaves over damaged leaves and purified air. Undamaged leaves attracted 52 % of F. intermedia from the East London variety, 62.5 % from the Port Alfred variety, 56 % from the Whitney Farm variety, 58 % from the Lyndhurst variety and 54.5 % from the Heather Glen variety in dual choice trials versus damaged leaves. Furthermore, a significantly higher proportion of F. intermedia were attracted to damaged leaves over purified air. Damaged leaves attracted 67 % of F. intermedia from the East London variety, 67 % from the Port Alfred variety, 65.9 % from the Whitney Farm variety, 65.3 % from the Heather Glen variety and 64.5 % from the Lyndhurst variety. Olfactometer bioassays were also conducted using purified standard compounds of four chemical compounds identified from essential oils, hexane was used as a positive control as it is reported to be an insect attractant in literature. Hexane was highly attractive to the mirids compared to three standard compounds caryophyllene, caryophyllene oxide and naphthalene at the rate of 80 %, 73 % and 80 %, respectively. The standard compounds tested against F. intermedia are major compounds contained by L. camara varieties and they have proven to have a repellent effect. This may indicate that after feeding by F. intermedia, the major compounds expressed by the plant varieties repel F. intermedia contributing to the invasiveness of this weed. The increased expression of hexane and caryophyllene after feeding may also indicate increased attraction to some insects, opening up the potential for third trophic level interactions in varieties where this is the case. This is the first study on the chemical composition of essential oils of L. camara in South Africa. Therefore, we recommend that where appropriate chemical profile studies of the invasive alien plants should be considered during host specificity testing, and the vital role of chemical compounds on agent-weed interactions must be taken into consideration with other factors before and after the biological control agents are released.
- Full Text:
- Date Issued: 2017
- Authors: Ngxande-Koza, Samella W
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4877 , vital:20739
- Description: Lantana camara L. (Verbenaceae) is one the most problematic invaders in South Africa invading forest edges, sand dunes, and shorelines by forming impenetrable thickets. Lantana camara invasions degrade natural biodiversity, reduce the value of land and consequently it has been a target for biological control, over the last 50 years in South Africa. Studies that have reported on chemical profile of Lantana camara have been conducted around the world but not in South Africa. Hence, the first aim of the current study was to identify the chemical baseline of L. camara varieties in the Eastern Cape, South Africa. Recent studies have shown that feeding by one of the agents released against L. camara, Falconia intermedia (Distant) (Hemiptera: Miridae), induces anti-herbivory response through increased leaf toughness and trichome density. A preliminary study conducted also reported the production of volatile chemicals by one variety, Whitney Farm, due to feeding by the mirids. Therefore, the second aim was to determine the induced changes in chemical compounds of L. camara varieties after feeding by F. intermedia. A third aim was to determine the effect these chemical compounds have on the behaviour of F. intermedia. To identify the chemical baseline of L. camara varieties, the essential oils of four L. camara varieties (East London, Port Alfred, Whitney Farm and Heather Glen) were analysed using gas chromatography mass spectrometry (GC-MS) and that resulted to the identification of 163 constitutive and 75 induced chemicals across the varieties tested. Lantana camara varieties showed different chemical classes but were highly dominated by terpenes. A great variation in the number of constitutive chemical compounds was found in all the varieties. There were 56 constitutive chemical compounds in the Whitney Farm variety, 41 in the East London variety, 36 in the Heather Glen variety and 30 in the Port Alfred variety. The Whitney Farm variety had the highest number (22) of unique constitutive chemicals identified when compared with other varieties. This indicates the chemical distinctiveness of the Whitney Farm variety from the other varieties. In the varieties tested, there were common chemical compounds identified in constitutive and induced (discussed below) states of the plants such as caryophyllene, hexane, naphthalene, copaene and a-caryophyllene. Besides naphthalene, the majority of chemical compounds in South African L. camara varieties were similar to compounds that have been identified across the world, suggesting that they are closely related. The expression of naphthalene in these varieties may be due to changes in the chemicals expressed over evolutionary time as predicted by the Novel Weapons Hypothesis. Amongst the varieties, a great variation in chemical compounds and their concentrations was shown in the induced states of the plants. The concentration of constitutive caryophyllene ranged from (3.13 - 15.7) %, to (4.02 - 11.10) % after feeding. The concentration of constitutive hexane ranged from (6.13 - 71.19) %, to (33.3 - 75.8) % after feeding. The concentration of constitutive naphthalene ranged from (0.21 - 4.79) %, to (0.92 - 2.11) % after feeding. The concentration of constitutive copaene ranged from (0.57 - 1.57) %, to (1.20 - 2.72) %. Lastly, the concentration of constitutive a-caryophyllene ranged from (1.18 - 9.03) %, to (0.78 - 5.48) % after feeding. The changes in chemical concentrations in lantana varieties indicated that feeding by the mirid on L. camara varieties causes an induction by either reducing or increasing the chemical concentrations. To determine the effect of the identified compounds on the behaviour of F. intermedia adults, olfactometer bioassays were conducted using a Y-tube technique. A significantly higher proportion of F. intermedia were attracted to undamaged leaves over damaged leaves and purified air. Undamaged leaves attracted 52 % of F. intermedia from the East London variety, 62.5 % from the Port Alfred variety, 56 % from the Whitney Farm variety, 58 % from the Lyndhurst variety and 54.5 % from the Heather Glen variety in dual choice trials versus damaged leaves. Furthermore, a significantly higher proportion of F. intermedia were attracted to damaged leaves over purified air. Damaged leaves attracted 67 % of F. intermedia from the East London variety, 67 % from the Port Alfred variety, 65.9 % from the Whitney Farm variety, 65.3 % from the Heather Glen variety and 64.5 % from the Lyndhurst variety. Olfactometer bioassays were also conducted using purified standard compounds of four chemical compounds identified from essential oils, hexane was used as a positive control as it is reported to be an insect attractant in literature. Hexane was highly attractive to the mirids compared to three standard compounds caryophyllene, caryophyllene oxide and naphthalene at the rate of 80 %, 73 % and 80 %, respectively. The standard compounds tested against F. intermedia are major compounds contained by L. camara varieties and they have proven to have a repellent effect. This may indicate that after feeding by F. intermedia, the major compounds expressed by the plant varieties repel F. intermedia contributing to the invasiveness of this weed. The increased expression of hexane and caryophyllene after feeding may also indicate increased attraction to some insects, opening up the potential for third trophic level interactions in varieties where this is the case. This is the first study on the chemical composition of essential oils of L. camara in South Africa. Therefore, we recommend that where appropriate chemical profile studies of the invasive alien plants should be considered during host specificity testing, and the vital role of chemical compounds on agent-weed interactions must be taken into consideration with other factors before and after the biological control agents are released.
- Full Text:
- Date Issued: 2017
Comparative localization studies of P.falciparum ADP-ribosylation factor proteins in P.falciparum parasites and hela cells using GFP tagged constructs
- Authors: Swart, Tarryn
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3537 , vital:20519
- Description: Expected release date-December 2018
- Full Text:
- Date Issued: 2017
- Authors: Swart, Tarryn
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3537 , vital:20519
- Description: Expected release date-December 2018
- Full Text:
- Date Issued: 2017
Density-dependent effects on body size, Philopatry, and dispersal in the Damaraland mole-rat (Fukomys damarensis)
- Authors: Finn, Kyle T
- Date: 2017
- Subjects: Fukomys damarensis , Fukomys damarensis -- Reproduction , Fukomys damarensis -- Growth , Rodents -- Reproduction -- South Africa -- Northern Cape , Rodents -- Growth -- South Africa -- Northern Cape , Rodent populations -- South Africa -- Northern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/50495 , vital:25993
- Description: Population density may exert changes in a variety of behavioural and physiological characters in animals. However, the effects of density-dependence and dispersal are poorly studied in subterranean rodents due to the difficulties involved in observing such effects in wild populations. Using the cooperative breeding Damaraland mole-rat (Fukomys damarensis) as a model species, the effects of population density on body size, growth rates, group size, recruitment, philopatry and dispersal were investigated at two sites (one with low density and one with high density) in the Northern Cape of South Africa. Group size, litter size and the probability of recapture were independent of population density. However, individual body size, recruitment, dispersal rate and dispersal distance were density-dependent. Individuals were significantly larger, juveniles exhibited a significantly higher growth rate, and juvenile recruitment was significantly greater when population density was low. At higher densities, significantly more individuals were lost between capture events which may be indicative of increased rates of dispersal. Mean dispersal distances in mole-rats were reduced at higher densities and increased at lower densities. While both sexes dispersed equally, males were significantly more likely to join an established colony and females created new burrow systems. In addition, four times as many single females were found when population density was low. The apparent differences in the study populations may be attributed to variable annual rainfall or food availability. The greater annual rainfall at the high-density site may provide better habitat conditions and therefore result in an increase in the population density and an increased dispersal rate due to the relaxed ecological constraints such as reduced energetic costs to burrowing. However, the larger body size found at the low-density site may indicate that the available food at that location is of better quality. This study revealed that mole-rats were able to disperse over 1km and therefore a much larger study area would be required in capture-mark-recapture studies to ensure the recapture of the majority of dispersers. Lastly, a surprising find of this study was that females may survive a solitary existence for over two years while awaiting the arrival of a mate.
- Full Text:
- Date Issued: 2017
- Authors: Finn, Kyle T
- Date: 2017
- Subjects: Fukomys damarensis , Fukomys damarensis -- Reproduction , Fukomys damarensis -- Growth , Rodents -- Reproduction -- South Africa -- Northern Cape , Rodents -- Growth -- South Africa -- Northern Cape , Rodent populations -- South Africa -- Northern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/50495 , vital:25993
- Description: Population density may exert changes in a variety of behavioural and physiological characters in animals. However, the effects of density-dependence and dispersal are poorly studied in subterranean rodents due to the difficulties involved in observing such effects in wild populations. Using the cooperative breeding Damaraland mole-rat (Fukomys damarensis) as a model species, the effects of population density on body size, growth rates, group size, recruitment, philopatry and dispersal were investigated at two sites (one with low density and one with high density) in the Northern Cape of South Africa. Group size, litter size and the probability of recapture were independent of population density. However, individual body size, recruitment, dispersal rate and dispersal distance were density-dependent. Individuals were significantly larger, juveniles exhibited a significantly higher growth rate, and juvenile recruitment was significantly greater when population density was low. At higher densities, significantly more individuals were lost between capture events which may be indicative of increased rates of dispersal. Mean dispersal distances in mole-rats were reduced at higher densities and increased at lower densities. While both sexes dispersed equally, males were significantly more likely to join an established colony and females created new burrow systems. In addition, four times as many single females were found when population density was low. The apparent differences in the study populations may be attributed to variable annual rainfall or food availability. The greater annual rainfall at the high-density site may provide better habitat conditions and therefore result in an increase in the population density and an increased dispersal rate due to the relaxed ecological constraints such as reduced energetic costs to burrowing. However, the larger body size found at the low-density site may indicate that the available food at that location is of better quality. This study revealed that mole-rats were able to disperse over 1km and therefore a much larger study area would be required in capture-mark-recapture studies to ensure the recapture of the majority of dispersers. Lastly, a surprising find of this study was that females may survive a solitary existence for over two years while awaiting the arrival of a mate.
- Full Text:
- Date Issued: 2017
Development and validation of an In Vitro Release Test (IVRT) to investigate the release of miconazole nitrate from topical cream formulations
- Authors: Purazi, Potiwa
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/65223 , vital:28711
- Description: Expected release date-May 2019
- Full Text:
- Date Issued: 2017
- Authors: Purazi, Potiwa
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/65223 , vital:28711
- Description: Expected release date-May 2019
- Full Text:
- Date Issued: 2017
Formulation, development and evaluation of lipid nanocarriers for minocycline hydrochloride
- Authors: Ranchhod, Janeeta
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/65234 , vital:28712
- Description: Expected release date-May 2019
- Full Text:
- Date Issued: 2017
- Authors: Ranchhod, Janeeta
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/65234 , vital:28712
- Description: Expected release date-May 2019
- Full Text:
- Date Issued: 2017
Identification of SNPs within the CYP2A6 enzyme of TNBC cell lines and the resulting change in activity
- Dingle, Laura Margaret Kirkpatrick
- Authors: Dingle, Laura Margaret Kirkpatrick
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/64349 , vital:28536
- Description: Expected release date-May 2019
- Full Text:
- Date Issued: 2017
- Authors: Dingle, Laura Margaret Kirkpatrick
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/64349 , vital:28536
- Description: Expected release date-May 2019
- Full Text:
- Date Issued: 2017