Composition and physiological roles of gut microbiota in the False Coding Moth (Thaumatotibia leucotreta)
- Authors: Richardson, Perryn Heather
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424457 , vital:72155
- Description: Gut microbiota can have a profound influence on host performance, behaviour and fitness. For False Codling Moth (FCM), Thaumatotibia leucotreta (Lepidoptera: Tortricidae), a major pest of citrus in South Africa, little work has been undertaken to date on gut microbe diversity or its influence on the host. This thesis aimed to i) characterise the gut microbiome of FCM under laboratory conditions and in FCM from the field, ii) and produce moths with reduced gut microbiota through egg dechorionation, which was followed by iii) the measurement of a suite of physiological traits, namely mass, survival and thermal stress in FCM from normal laboratory, dechorionated laboratory and field collected larvae that may be indicative of overall field performance. We aimed to directly test the hypothesis that gut microbial diversity partly determines insect performance and fitness by measuring its effects on growth, development, and tolerance to cold temperatures in FCM. FCM eggs that underwent dechorionation with sodium hypochlorite had an overall effect on larval survival, egg morphology and both larval and adult moth physiological measures. Increasing concentrations of sodium hypochlorite significantly decreased insect survival, (𝜒2(1, n = 10 850) = 21.724, p-value < 0.0001), with a concentration of ≈3.69% as the concentration limit (p-value < 0.001). Successful dechorionation of FCM was achieved with a wash of sodium hypochlorite at around 3.69% concentration and was visually confirmed by reduction of FCM egg surface area, (𝜒2(25, n = 260) p-value < 0.0001) and Scanning Electron Micrographs of the egg morphology. The gut microbiome of FCM from the different focus treatments was successfully characterized. Identification of the dominant bacterial families in these microbiomes revealed Xanthobacteraceae, Beijerinckiaceae and Burkholderiaceae in both the laboratory reared and field collected larvae, which suggests their systematic association with T. leucotreta. The most abundant genera were revealed as Bradyrhizobium, Methylobacterium and Burkholderia-Caballeronia-Paraburkholderia. Comparison of larval mass showed that treatment (dechorionated or not) had a significant effect on larval mass (𝜒2(2, n = 230) = 22.703, p-value < 0.001), field larvae were heavier than both control larvae and larvae with a disrupted gut microbiome. However, adult insects with a disrupted gut microbiome had more mass than individuals from the control and field-collected larvae with intact gut microbiomes (𝜒2(2, n = 230) = 39.074, p-value < 0.001). Despite the difference in mass between larval treatments, there was no significant difference in relative protein (𝜒2(2, n = 24) = 5.680, p-value = 0.06), carbohydrate (𝜒2(2, n = 24) = 3.940, p-value = 0.14) or lipid (𝜒2(2, n = 24) = 6.032, p-value = 0.05) content between individuals from the control and dechorionated treatments and field-collected individuals. Turning to thermal physiology, insects collected from the field took significantly longer to recover from chill coma than both laboratory treatments with intact and disrupted gut microbiomes (𝜒2(2, n = 129 = 39.659, p-value < 0.001). In addition, exposure to cold stress showed that treatment had a significant effect on insect mortality (𝜒2(2, n = 272) = 9.176, p-value = 0.01), with individuals from the control and dechorionated treatment being less likely to die after experiencing cold stress compared to field-collected individuals. Differences in the mass and thermal tolerance of insects with intact and disrupted gut microbiota suggest that gut microbiota may play an important role in the cold performance of T. leucotreta, and these findings constitute the basis for future molecular work on the functions of these bacterial taxa. This research highlights the need for consideration of the effects of T. leucotreta microbiome in current pest control programs. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
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Marine plastic pollution impacts on ecosystem services and livelihoods in South Africa: a review and stakeholder perceptions
- Authors: Yose, Papama
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424743 , vital:72180
- Description: Marine plastic pollution is one of the major environmental problems globally, with adverse impacts on marine ecosystem services, functions and benefits and people. Yet, compared to plastic pollution impacts on terrestrial ecosystems, the impacts of plastic waste on marine ecosystems are little studied and poorly understood in South Africa. In response, research interest on the ecological and economic impacts of marine plastic pollution has grown rapidly but socio-cultural impacts remain poorly conceptualized and little understudied. This trend is arguably driven by the dominance of a quantitative paradigm which is arguably inadequate to respond to dynamic socio-cultural issues and contexts. Further, in South Africa, marine ecosystems are used by diverse groups of people ranging from subsistence users, tourism operators, commercial fishers and recreational users, meaning that addressing marine plastic pollution requires a multi-stakeholder approach. However, few studies explore the perceptions of diverse stakeholders regarding marine plastic pollution, its impacts and potential interventions. Against this background, the objective of the thesis are twofold: (a) to argue for a more nuanced understanding of marine plastic pollution impacts on human well-being in order to strengthen conceptualization of impacts that goes beyond direct and quantitatively measured impacts (ecological, health and economic) but considers the socio-cultural dimensions of impacts (lifestyle disruptions, mental health costs and cultural and heritage impacts), and (b) examine stakeholders’ perceptions of marine plastic pollution, its impacts and potential interventions in South Africa. Drawing on case studies, this study provides a working definition of the socio-cultural impacts of marine plastic pollution and a conceptual framework for categorizing the impacts. It highlights three dimensions of the socio-cultural impacts of marine plastic pollution (lifestyle, mental health, and cultural and heritage impacts). It also illustrates connections between marine ecosystems and marine-based livelihood activities with the social and cultural dimensions of human wellbeing to show the links between marine environments and socio-cultural contexts. Concerning stakeholder perceptions, the study showed a tendency to associate marine plastic pollution with immediate, noticeable and easily quantifiable ecological impacts such as entanglement and ingestion of marine organisms, economic impacts and social impacts (e.g., loss of aesthetic appeal). However, secondary impacts such as loss of marine biodiversity and social impacts such as human health, were largely unknown. Moreover, there was heterogeneity in perceptions of marine plastic pollution impacts among the stakeholder groups. Prospective studies researching marine plastic pollution impacts should consider divergent and diverse perceptions of different stakeholders to capture the real costs of marine plastic pollution. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2023
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