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Chemical analysis and biological activities of crude extracts and essential oil of selected medicinal plants from the Eastern Cape, South Africa, and Volta Region of Ghana

  • Authors: Agbo, Irene Adzo
  • Date: 2023-12
  • Subjects: Medicinal plants , Lantana camara , Peptic ulcer -- Treatment , Traditional medicine - South Africa -- Eastern Cape , Traditional medicine -- Ghana
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62431 , vital:72728
  • Description: Lantana camara and Khaya grandifoliola extracts are among many plants found traditionally effective for the treatment of wounds and ulcers. This study assessed the phytochemical content, isolation and identification of single compounds from methanol and ethyl acetate extracts of Lantana camara and Khaya grandifoliola. Further, the bioactivity testing including antioxidant, antimicrobial and cytoxicity of the extracts was done to confirm the wound healing potential discovered by the traditional healers. Materials and methods: Extraction was done successively using maceration method with 100 % ethyl acetate and 100 % methanol with a biomass-to-solvent ratio of 1:3 (w/v) to obtain L. camara ethyl acetate extracts of berry (ELB), flower (ELF) and leaf (ELL) and methanol extracts of MLB, MLF, MLL and K. grandifoliola ethyl acetate extracts of leaf (EKL), root (EKR) and stem bark (EKSB) and methanol extracts of MKL, MKR, MKSB respectively. L. camara leaf essential oil (EO) was extracted using the hydro-distillation method with a Clevenger apparatus. Total phytochemical content was assessed for each extract using spectrophotometric methods and a calibration curve of standards: bromocresol green method with atropine; Folin–Ciocalteu colorimetric method with gallic acid, aluminium chloride colorimetric method with quercetin and concentrated sulphuric acid chloroform with linalool for total alkaloid, phenolic, flavonoid and terpenoid contents respectively. Single compound isolation and purification was conducted using chromatographic techniques. Elucidation of single compounds was done using spectrometric method, high resolution- mass spectrometry, and one and two-dimensional (1D and 2D)-NMR. Stereochemistry of each compound was confirmed using electronic circular dichroism spectra. A Crystalline compound was identified by single crystal X-ray diffraction using CuKα-radiation. In vitro bioactivities were assessed with methods such as 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide, free radical scavenging activity of 1,1-diphenyl-2-picrylhydrazyl, inhibitory effect on nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages, and 96-well plate micro dilution for cytotoxicity, ant-inflammatory and antimicrobial activity testing. Results: Methanol extracts of both plants retained high phytochemical concentrations of all the phytoconstituents investigated compared with the ethyl acetate extracts which retained lower concentrations. The results of the L. camara methanol extracts include; total alkaloid content (TAC) (2.05±0.18, 1.87±1.54 and 2.60±1.10 mg AEQ/100 mg); total phenolic content (TPC) (14.05±4.04, 34.59±3.01 and 18.58±1.87 mg GAEQ/100 mg); the total flavonoid content(TFC) of flower (12.45±1.87, 20.41±2.69 and mg QEQ/100 mg); total terpenoids (TTC) (20.74±2.34, 20.74±2.34 and 15.97±1.19 mg LIN EQ/100mg) of MLB, MLF and MLL respectively. Whereas that of the K. grandifoliola methanol extracts include; TAC (7.32±0.14,8.49±0.34, 10.67±0.22 mg AEQ/100 mg); TPC (37.49±1.40, 44.41±0.69, 53.57±1.50 mgGAEQ/100 mg); TFC (6.54±0.55, 9.58±0.89 and 10.26±0.92 mg QEQ/100 mg); TTC(10.16±1.41, 35.78±2.14 and 23.45±1.76 mg LIN EQ/100mg) of MKL, MKR and MKSB respectively. The major components of essential oil, out of the 71 constituents identified include Davanone D (32.91 %), Caryophyllene (5.07 %), Nerolidol 2 (3.56 %) and GermacreneD (3.13 %). Compounds 3.47 was isolated from the methanol extract of L. camara flowers. This compound is reported for the first time from the L. camara flower extract. Two compounds, compounds 4.23, and 4.26, were isolated from the methanol extract of K. grandifoliola roots, compound 4.22 was isolated from the ethyl acetate root extract while compounds 4.24 and 4.25 were isolated from the ethyl acetate stem bark extract as isomers in a mixture. Compounds 4.22 and 4.23 are reported from K. grandifoliola root for the first time. The isolated compounds (compounds 3.47 and 4.23) were nontoxic to the Vero cell line and this may contribute to possible stimulation of cell proliferation, promoting wound healing. Cytotoxicity describes extract virulence to Vero cell line. MLF and ELB were found nontoxic even at the highest concentration of 200 μg/mL. The MKSB and MKR, as well as the EKSB were nontoxic. Antioxidant activity results, described by the percentage inhibition in the DPPH assay, showed that MLF and MKSB had the highest antioxidant activities compared with the ascorbic acid standard, with IC50 of 38.68±5.09 and 37.03±11.95 μg/mL for L. camara and K. grandifoliola respectively. ELB exhibited a significant anti-inflammatory activity inhibiting NO• radical generation in the LPS-stimulated RAW 264.7 macrophages at concentration ranging from 50 and 100 μg/mL. EKSB and MKR showed significant anti-inflammatory activity at 100 and 200 μg/ml respectively. ELL and ELF demonstrated potent growth inhibition against S. pyogenes with an MIC value ≤ 0.125 mg/mL, while the MICs of the ELB and MLL were 0.5 mg/mL and 2 mg/mL respectively. MKSB and MKR and EKSB extract exhibited an effective growth inhibition against S. aureus with MIC of 1 mg/mL. The growth of S. pyogenes was supressed by both ethyl acetate and methanol extracts of all plant parts tested with MIC ranging from 0.25–2 mg/mL. Conclusion: The potent bioactivity shown in the results of the cytotoxicity, antioxidant activity, anti-inflammatory and antimicrobial activity testing, and the nontoxic singlecompounds of L. camara and K. grandifoliola extracts led to the conclusion that the two plants had wound healing potential. The study therefore confirmed their traditional uses for treatment of wounds. , Thesis (PhD) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2023
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  • Date Issued: 2023-12

Dietary assessment of Cape Gannets (Morus capensis) and African Penguins (Spheniscus demersus) in Algoa Bay using molecular scatology

  • Authors: Bowmaker - Falconer, Kezia , Von Der Heyden, Sophie
  • Date: 2023-12
  • Subjects: Gannets , Gannets -- Food , Sea birds -- South Africa -- Bird Island, Algoa Bay.
  • Language: English
  • Type: Master's theses
  • Identifier: http://hdl.handle.net/10948/62420 , vital:72675
  • Description: Seabirds are important marine sentinels, providing valuable information about ecosystem health and condition. Dietary studies of seabirds can inform on ecosystem functioning through identifying the condition and changes occurring in their surrounding environment. Dietary studies are also crucial as they can identify threats that seabirds face. Until recently, the diets of southern African endemic and Endangered Cape Gannets Morus capensis and African Penguins Spheniscus demersus were solely investigated through stomach content analyses, which allows for the identification and quantification of prey remains. To overcome some of the limitations associated with stomach content analysis, stable isotope and fatty acid analyses were proposed. However, these methods still require bird handling and thus impose some stress on the birds. DNA metabarcoding of faecal matter shows promise as a completely noninvasive method for seabird dietary studies. This thesis first examined whether DNA metabarcoding could be used to determine the diet of Cape Gannet and African Penguin breeding in Algoa Bay, South Africa. Then, this method was used to investigate the influence of intrinsic (both seabird species) and extrinsic (African Penguins) factors on the diet of the two seabird species. For the first data chapter, stomach content samples and faecal matter were collected concurrently from breeding Cape Gannets and African Penguins on Bird Island. DNA was successfully extracted from most faecal matter samples and the primers (18S_SSU, 16S_Fish) used for group, taxa and fish prey identification allowed amplification of prey DNA for both study species. A similar piscivorous diet was determined by both dietary methods for Cape Gannets with Sardine Sardinops sagax and Atlantic Saury Scomberesox saurus dominating the diet. Sardine also dominated the African Penguin diet in both dietary methods. In addition, DNA metabarcoding in African Penguins revealed a more diverse diet compared to the one determined by stomach content analysis. This was likely due to the limitations on the allowed number of stomach content samples to be collected for that Endangered species. Non-invasive DNA metabarcoding of faecal matter was then used to investigate whether the diet varied with breeding stages (incubation, chick-rearing) and age classes (chick-rearing adults, chicks) in both seabird species, but also breeding colonies (Algoa Bay: Bird Island, St Croix Island) in African Penguins. DNA metabarcoding revealed that breeding stages influenced the diet of Cape Gannets and African Penguins on Bird Island where prey species diversity was greater for incubating adults. On the contrary, the diet of incubating and chick-rearing penguins on St Croix Island was similar. When comparing the diet of African Penguins between Bird Island and St Croix Island, there was an increased overlap in diet composition between incubating and chick-rearing adults on St Croix Island when compared to Bird Island. Importantly, DNA metabarcoding indicated an increase in fishery interactions in Algoa Bay, particularly for Cape Gannets and the hake Merluccius spp. fishery, where the contribution of hake to chick-rearing adults and chick diet was considerably greater than incubating adult diet. In this study, I showed that DNA metabarcoding of faecal matter provides an opportunity for non-invasive dietary studies in two southern African endemic and Endangered seabird species. Incorporating non-invasive methods as a complement to other dietary methods for monitoring seabird trophic ecology is crucial when considering the worsening status of several seabird species. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
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  • Date Issued: 2023-12

Effects of selected natural flavonoids on an optimized insulin resistance induction model on myotubes and hepatocytes in vitro

  • Authors: Van de Venter, Ruben
  • Date: 2023-12
  • Subjects: Flavonoid , Insulin resistance , Myotubes and hepatocytes
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/63049 , vital:73085
  • Description: Introduction: As the third largest life-threatening non-communicable disease, further research to improve T2DM treatment remains pivotal. The approved insulin sensitizing agents, metformin and thiazolidinediones, although effective, have an array of adverse effects. This leads to the growing need for safer insulin sensitizing agents. Aims: To induce insulin resistance, an insulin resistance induction (IRI) model consisting of palmitic acid (PA), fructose, and dexamethasone (DEX), was optimized. The model was utilized to compare the insulin sensitizing efficacy of chrysin, apigenin, luteolin, and quercetin. The most effective flavonoid was extrapolated based on the hydroxylation hypothesis and relevant SAR. The flavonoid was combined with metformin to evaluate potential insulin sensitizing synergism. Methods: C3A hepatocytes and L6 myotubes were exposed to the IRI models: IR1, IR2, and OIR, for 24 and 48h. Morphological features indicative of insulin resistance were monitored through high-content analysis (HCA). Thereafter, cells were exposed to OIR and concurrently treated with the selected flavonoids. Cytotoxicity, oxidative stress (OS), mitochondrial content, mitochondrial membrane potential (MMP), and lipid accumulation, were once again evaluated through HCA. Subsequently, quercetin was combined with metformin (QM); insulin resistant conditions established through OIR exposure, and QM administered as prophylaxis. The efficacy of QM was determined through changes in phosphorylated-Akt, glucose uptake, and gluconeogenesis. Results: IR1 and IR2 demonstrated excessive potency, whereas OIR consistently generated insulin resistant C3A/L6 cells by increasing OS (↑14%/20%), lipid accumulation (0.7-fold/1.5-fold), and decreasing MMP (↓4%/6%). Of the selected flavonoids, quercetin was most effective at ameliorating each of these parameters. Furthermore, QM demonstrated potential insulin sensitizing synergism, as it improved Akt phosphorylation (Thr308: ↑31%/↑17%; Ser473: ↑29%), glucose uptake (↑10%/ 5%), and suppressed hepatic gluconeogenesis (15↓%) more effectively compared to monotherapy. Conclusions: OIR is an effective model for the induction of complete early-stage insulin resistance in myotubes and hepatocytes. Quercetin demonstrates exceptional antidiabetic activity due its unique expression of hydroxyl groups. Lastly, QM, comparative to monotherapy, improves insulin sensitivity with enhanced efficacy. , Thesis (Ma) -- Faculty of Health Science, 2023
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  • Date Issued: 2023-12

Evaluating antidiabetic properties of selected African medicinal plants in a cell-based model

  • Authors: Sirkhotte, Saeedah , Reddy, Shanika
  • Date: 2023-12
  • Subjects: Medicinal plants -- South Africa , Insulin resistance , Gluconeogenesis
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62600 , vital:72827
  • Description: The World Health Organisation lists diabetes as one of the major non-communicable diseases affecting the world, and its prevalence is expected to increase rapidly. Type II diabetes mellitus (T2DM) is characterised by insulin resistance and impaired blood glucose control. T2DM is of growing concern within South Africa, with 10.8% of the population currently diagnosed. The popularity, as well as availability, of traditional plant-based medicine in South Africa, might provide a solution. Antidiabetic potential is commonly tested by in vitro assays, however the methods to test this potential via gluconeogenesis are limited. This project aimed to optimise an in vitro hepatic gluconeogenesis model. In addition, this project aimed to determine the antidiabetic properties of Prunus africana, Hypoxis stellipilis, and Eriocephalus africanus by in vitro analysis. These properties were examined in human hepatoma cells (C3A cell line) and rat pancreatic cells (INS1 cell line) and methods included: cytotoxicity analysis, Amplex® red glucose oxidase assay, antibody staining, gene expression analysis by RT-qPCR, oxidative stress analysis, and calcium signaling for insulin release. Insulin resistance was successfully induced by exposing C3A hepatocarcinoma cells to a combination treatment of 50 μM dexamethasone, 1.25 mM fructose and 0.125 mM palmitic acid for a period of three days. Thereafter, gluconeogenesis was assessed using the Amplex® red glucose oxidase assay. The established model was effective in inducing insulin resistance and upregulating gluconeogenesis. Of the tested plant extracts, H. stellipilis showed the most potential as an antidiabetic treatment. It had low toxicity, significantly decreased hepatic glucose production and reduced the amount of phosphoenolpyruvate carboxykinase (PCK) and well as PCK gene expression, and reduced lipid content and reactive oxygen species (ROS) in the C3A cell line. H. stellipilis increased calcium signalling in INS1 rat insulinoma cells, however there was a decrease in expression of genes for insulin and glucose transporter 2 after 6 hr exposure. H. stellipilis appears be beneficial as an antidiabetic treatment. Although antidiabetic studies have been done on other Hypoxis species, this is the first study on the effects of H. stellipilis on gluconeogenesis and diabetes. , Thesis (MSc) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2023
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  • Date Issued: 2023-12

Experiences of mothers who are on tuberculosis treatment While caring for their children in Nelson Mandela Bay health district

  • Authors: Mwadiwa, Chiedza
  • Date: 2023-12
  • Subjects: Caring experiences -- Nelson Mandela Bay , Tuberculosis treatment
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62935 , vital:73071
  • Description: Women and mothers have an important role to play as caregivers of their children. However, mothers are often faced with challenges with fulfilling this important role. Many women value the caring role as a mother over other areas of their lives, and for ill women, such as those with tuberculosis (TB) and those on TB treatment, it can be difficult to relinquish maternal responsibility. Indeed, many mothers in South Africa and other low- and middle-income countries develop TB during pregnancy and postpartum because their immune systems are compromised. For a mother who is chronically ill, carrying out a mother’s caregiving role presents a range of challenges. However, few studies have addressed this problem or explored the experiences of such mothers. The purpose of the study was to understand the experiences of mothers who are on TB treatment while caring for their children in order to establish their support needs and thereby develop recommendations to assist CHWs, nurses and nurse managers at primary healthcare (PHC) clinics to provide optimum care to such women. The Socio-Ecological model was used as the theoretical lens to explore the phenomenon under investigation. A qualitative, exploratory-descriptive research design was identified as most suited to respond to the research question. Purposive sampling was used to select both the participants and the clinics in the Nelson Mandela Bay Health District (NMBHD) for the purpose of this study. The population was mothers on TB treatment caring for their children (aged five years or younger) who were attending either one of the two of the largest PHC clinics in Sub-district C in the NMBH. Altogether ten mothers on TB treatment caring for their children took part in individual semi-structured interviews conducted telephonically. Ethical clearance was obtained from Nelson Mandela University and permission was obtained from the Department of Health (DoH), district office, operational managers at the respective clinics, and the participants involved in the study. Trustworthiness was enhanced by ensuring credibility, transferability, dependability, and confirmability. Ethical principles were upheld throughout the course of this study namely respect for persons, beneficence and justice. All interviews were recorded and transcribed, and the data was analysed using Tesch’s method of data analysis to identify the themes. Three themes came out of the data, namely: Theme 1: Mothers expressed having to navigate a range of physical and psychosocial challenges while caring for their v children; Theme 2: Mothers struggled to perform motherly roles while on TB treatment; Theme 3: Mothers described their relationships with their family, community members and clinic health practitioners. The experiences of the participants were described using direct quotations from the interviews followed by a literature control used to verify the participants’ experiences. Thereafter, the findings were used to develop recommendations to optimise the care and support of mothers on TB treatment who are caring for their children. Four recommendations were developed: Recommendation 1: Prepare and support mothers with TB to care for themselves and their children; Recommendation 2: Assist CHWs and nurses to care and support mothers with TB and their children; Recommendation 3: Prepare and support family members to support mothers with TB and their children and Recommendation 4: Educate community members regarding the needs of mothers with TB. There is limited existing research on mothers who are on TB treatment while caring for their children. This research study helped to fill this gap, particularly in an urban Eastern Cape context. The findings illustrated mothers’ physical and psychosocial challenges, as well their role-related challenges and finally the challenges related to the various spousal, family, community and clinic relationships that influence their experience of caring for their child while on TB treatment. , Thesis (MA) -- Faculty of Health Science, 2023
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  • Date Issued: 2023-12

Experiences of psychological practitioners when working with Isixhosa speaking minors in the Eastern Cape.

  • Authors: Matushe, Masakhane
  • Date: 2023-12
  • Subjects: language barriers -- Eastern Cape , Psychological practitioner
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62884 , vital:73062
  • Description: Language barriers continue to pose challenges in education and the health sector where psychological assessments and tests are administered in a language that is different from that of the client. The implication this has on the interpretations of test-taker scores may be bias against those whose first language is not English or Afrikaans, which are two of the primary languages majority of the tests currently used in the South Africa are normed against. This study aimed to explore the implications of the current language repertoires on test-taker performance. This was achieved through exploring the experiences of psychological practitioners who use English language psychological tests on isiXhosa speaking child clients in the Eastern Cape. The methodology followed an exploratory-descriptive, qualitative design, adopting Lincoln and Guba's model of trustworthiness. The researcher employed a qualitative method with an exploratory-descriptive research design, and participants were recruited by utilizing a purposive sampling strategy. Thematic analysis was used as a means of data analysis,and the following themes emerged and were discussed: The use of translators & interpreters, and the challenges thereof; practitioner’s own language proficiencies and limitations; cultutral influence and langaage exposure of clients; Adaptation as well as revision of decolonization of testing measures; and training psychological practitioners. , Thesis (Ma) -- Faculty of Health Science, 2023
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  • Date Issued: 2023-12

Exploring culturally adapted psychological interventions for mental illness in South Africa: A scoping review

  • Authors: Mbatha, Andiswa
  • Date: 2023-12
  • Subjects: Cultural Adaptation , Evidence-Based Treatments
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62898 , vital:73065
  • Description: Culturally appropriate interventions are crucial for its universal applicability. Although it is well documented that mental health practitioners need to consider cultural factors when working with clients, there is a paucity of research concerning culturally adapted psychological treatments within South Africa. Mental Health practitioners use borrowed Westernised methods of psychological interventions, whose effectiveness has been proven to work on people with different values and belief systems from those of South Africa. This study sought to map the literature on culturally adapted psychological interventions in the South African context. The main research question of the study is: “According to the literature, what are the culturally adapted psychological interventions available for use in the South African context?” Employing the scoping review methodology, published literature was sourced from various electronic databases. The data is presented in a table format using the thematic framework. The study found that out of 250 records that were retrieved, we identified nine articles describing four distinct interventions in treating three mental illnesses in South Africa. Cognitive behaviour therapy (n = 3), behaviour activation (n=2) mindfulness-based stress reduction (n=2) interpersonal therapy (n=1) and Motivational interviewing (n=1) surfaced as culturally adapted psychological interventions to treat people with depression, trauma and substance use disorder. Improvement in symptoms was a common result of all therapies. The potential benefit of the study is to provide recommendations that will inform further research and clinical practice. , Thesis (Ma) -- Faculty of Health Science, 2023
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  • Date Issued: 2023-12

Exploring the psychological and emotional understanding of Amagqirha within the Eastern Cape province, South Africa

  • Authors: Ncapayi, Abongile
  • Date: 2023-12
  • Subjects: traditional healers , Indigenous Knowledge , Cultural religion
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62947 , vital:73074
  • Description: Indigenous knowledge systems influence mental health understanding and treatments. Amongst the Xhosa’s (amaXhosa), a person may receive a ‘calling’ from the ancestors, usually associated with symptoms that would be characterized as a mental illness. However, the symptoms experienced during the calling, usually dissipate once training has been received. One of the categories within traditional healers are amagqirha, also known as diviners. These are individuals who receive the ‘calling’. Thereafter, they undergo extensive training and become blessed with clairvoyant “powers” to divine and act as mediators between the living and the dead. This study aimed to explore the psychological and emotional understanding of amagqirha of the Xhosa culture, within the Eastern Cape, South Africa. In doing so, the study centralizes the experiences of individuals within cultural spaces, allowing for commentary on the psychological and emotional understanding of the amagqirha. Furthermore, this study focused on the work that amagqirha do in communities and how this ultimately affects them. This study sought to understand the experiences and perceptions of the individuals who are affected by this ‘calling’. The researcher employed a qualitative method with an exploratory-descriptive research design. Ten participants were recruited by utilizing a purposive snowball sampling strategy. Data was collected using a semi-structured interview due to the sensitivity of the demographic of the study. Ethics were adhered to. Additionally, trustworthiness was guided by Lincoln and Guba’s (1985) four constructs: credibility, dependability, transferability, and confirmability. Thematic analysis was used as a means of data analysis, as presented by Braun and Clarke (2006). The themes identified were: The collateral damage/profuse repercussions cast upon by the ancestors due to inaction (either by them or those that case before them), the severity of the emotional and psychological effects is equally prevalent prior and during their journey of vi ubugqirha, methods that may assist in alleviating the emotional and psychological symptoms, understanding of mental health, awareness of their own mental health status, and help-seeking (psychological services). The main findings suggested that there is a general understanding of the term ‘mental health’ amongst amagqirha. Additionally, the findings revealed that amagqirha experienced emotional and psychological symptoms/effects, prior to and during the journey of ubugqirha - these were due to the lack of understanding and fear. Furthermore, the findings suggesting that amagqirha embarked on various help-seeking measures, i.e., seeking psychological services or leaning into seeking guidance from their ancestors. However, they were met with barriers as well, i.e., finding a mental health practitioner with awareness on African spirituality, and dealing with the stigma that comes with help-seeking. , Thesis (Ma) -- Faculty of Health Science, 2023
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  • Date Issued: 2023-12

Field mapping and geochemistry of lower Jurassic sediments and volcanics of the upper Karoo supergroup near Ha Mosi, Lesotho mountains

  • Authors: Valashiya, Khaya
  • Date: 2023-12
  • Subjects: Sediments (Geology) -- South Africa -- karoo basin , Geochemistry , Geological mapping
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62687 , vital:72925
  • Description: Geological mapping in the Lesotho-Drakensberg Mountains spans over 100 years with du Toit and Stockley being the major contributors to the published maps and to the understanding of emplacement of the Karoo Large Igneous Province (KLIP) in the countries of South Africa and Lesotho. Despite the detailed previous work, there remains limited data on paleoenvironmental changes at the contact between the sedimentary and volcanic sequences of the upper Karoo Supergroup. This study aims to map the lithostratigraphic boundaries, volcano-sedimentary facies and faults near Ha Mosi in southern Lesotho. A total 48 rock samples were collected for petrography, XRF and ICP-MS analyses. The new results were used to characterize the local chemostratigraphy and to propose new regional correlations across Lesotho and South Africa. In this thesis, eight lithostratigraphic units were defined with a total thickness of 550 m. The first two units (Units 1 and 2) comprise massive and bedded sandstones, interpreted as possible floodplain and ephemeral lacustrine deposits; these are ascribed to the upper Stormberg Group. These units are unconformably overlain by locally preserved thin basaltic (Unit 3) flows that are characterised by high Zr/Nb and Zr/Y ratios; and low Ti/Zr and P/Zr ratios similar to the Golden Gate Unit of the Drakensberg Group. The lava flows are overlain by bedded sandstone ascribed to Unit 4 and volcaniclastic breccia ascribed to Units 5 and 6. Unit 6 consists of a monolithic breccia that is composed of angular sandstone clasts similar to those of the underlying Stormberg Group. The monolithic breccia transitions upwards into a massive megaclastic clast-supported volcanic breccia, which consists of angular to sub-rounded basaltic and doleritic boulders. Petrified wood fragments are found within the megaclastic breccia, indicating the presence of vegetation during deposition and enhanced preservation. Unit 6 laterally grades into Unit 5, which is characterised by poorly bedded breccia composed of angular sandstone, mudstone clasts with minor basaltic and doleritic clasts and, breccia intraclasts. The breccia units are often associated with reworked sediments interpreted to be deposited by fluvial systems. The breccia is conformably overlain by basaltic lava flows (Unit 8) characterised by high Ti/Zr and P/Zr ratios and moderate to low Zr/Y and Zr/Nb ratios compared to the Unit 3 lavas and is similar to that of the Sani Pass Unit of the Drakensberg Group. The basaltic sequences in the Ha Mosi studied area preserve both pahoehoe textures and pillow lavas, indicating subaerial and subaqueous volcanism. The studied lithologies are intruded by gabbro and dolerites of the Karoo Dolerite Suite at 183 Ma. This stratigraphy records the transition from sedimentation in the Karoo Basin through to a dominantly volcanic succession, which has importance in terms of the Toarcian extinction at ca. 182 Ma. Mapped faults and associated fractures are orientated predominantly NW-SE with the hanging walls moved maximum 80 m to the south. The different dykes and fault structures possibly relate to the Weddel Triple Junction that developed during the Early Jurassic break up of Gondwana, between 200 and 180 Ma. The mapped lavas show that the magma source was a chemically heterogenous mantle that was subjected to different degrees of partial melting, with the introduction of small-scale chemical heterogeneities. The geodynamic setting is comparable to the Afar Triangle of north-east Africa where active tectonics created a large-scale NW-SE orientated fault system in response to crustal thinning and rifting. In both the Afar and Lesotho, vertical movements created lowlands that allow for the preservation of volcanic breccia and basalts with pillow lavas. These findings show that existing geological models in the Drakensberg-Lesotho Mountains can be improved upon detailed field mapping and geochemistry. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
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  • Date Issued: 2023-12

Functional traits and potential physical drivers of offshore benthic epifauna on the west and south coast of South Africa

  • Authors: Van Stavel, Jordan , Morris, Tamaryn
  • Date: 2023-12
  • Subjects: Marine ecology , Marine biology -- South Africa , Oceanography -- South Africa
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62698 , vital:72931
  • Description: Offshore benthic ecosystems (> 200 m depth) are challenging to access and remain poorly studied globally. Epifaunal invertebrates comprise a critical trophic level in benthic ecosystems and can serve as indicators of the overall health and functioning. There is limited knowledge of South Africa’s offshore marine environment and benthic ecosystem functioning due to limitations in funding, resources, and the lack of suitable science-based monitoring tools which are vital for sustainable management into the future. The aim of this research was to improve the understanding of epifaunal functioning as well as their potential physical drivers in 13 South African offshore biogeographic ecotypes. The objectives of this study were threefold: 1) to investigate the functional composition of benthic epifauna from 13 biogeographic ecotypes, 2) to quantify the Functional Diversity (FD) associated with epifaunal traits between the different assemblages (i.e. biogeographic ecotypes), and 3) to evaluate the relationship between physical variables and epifaunal functional traits at the biogeographic ecotype level. Biological trait-based approaches were applied to a subset of 80 benthic epifaunal species collected from 909 offshore stations along the west and south coasts of South Africa. Nine biological traits (associated with life history, morphology and behavioural characteristics exhibited by each species) were classified into 39 modalities and weighted with biomass. Community Weighted Means (CWMs) were used to evaluate the benthic epifaunal functional trait composition of the 13 biogeographic ecotypes. Functional composition across the 13 ecotype assemblages on the west and south coast appeared to be dominated by species with similar trait compositions, such as large, long-lived, surface crawling/burrowing/filter-feeding epifauna with medium to no mobility, however, their percentage of contribution to trait expression (CWMs) was higher on the west coast. Functional diversity indices (alpha and beta) showed higher overall FD for west coast ecotypes, with most ecotypes on the continental shelf (except slopes and canyons) being significantly different (p<0.05) from ecotypes on the south coast. The global RLQ (three-table co-inertia) test did not reveal a significant relationship between biomass and physical variables or between biomass and traits (p>0.05). The fourth-corner method was used to test the significance of individual traitenvironment relationships. The fourth-corner results were similar overall to the RLQ analysis, revealing that three physical variables (temperature, depth, and fluorescence) were significantly correlated to two trait modalities belonging to the feeding mode filter-feeders (FM1) and scavengers (FM5). Scavengers were positively correlated with depth and negatively with temperature, while filter-feeders were positively correlated with fluorescence levels. This indicated that the presence of scavenging epifauna increased with a decrease in temperature and an increase in depth. While a positive significant correlation between filter-feeders and fluorescence suggested their potential tolerance or preference for environmental conditions with high levels of fluorescence. This research was the first such study to explore the functional composition and diversity of benthic epifauna and their relationship with potential physical drivers in offshore west and south coast benthic biogeographic ecotypes of South Africa. The application of new tools to quantify the functional diversity of epifauna as indicators of ecosystem health, and their potential physical drivers. This provided a platform on which to advance our understanding of benthic communities and the roles they play in ecosystem functioning under changing environments. Functional trait-based approaches such as those applied in this study can provide us with vital information on the relationship between biodiversity, ecosystem functioning, and physical drivers. Environmental stressors and changing climate patterns threaten to impact marine ecosystems and their functioning. Benthic epifauna are especially sensitive to changes in their environment and these fluctuations could potentially lead to the loss of certain benthic functionality, altering the thresholds these ecosystems have to response to disturbances. This undermines the stability of these ecosystems which can have ripple effects on the health of these ecosystems and their ability to provide the ecosystem services humans dependent on. Having suitable tools to track current and predict future changes will therefore be vital to inform management and conservation strategies for sustainable ocean utilisation. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
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  • Date Issued: 2023-12

Honouring the Dead: The Psychological Consequences of the COVID-19 Lockdown Restrictions on Burial Practices and Rituals, amongst AmaXhosa from the Eastern Cape, South Africa

  • Authors: Sibam-Twalo, Zimkhitha
  • Date: 2023-12
  • Subjects: Death and burial , COVID-19 Lockdown
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/63005 , vital:73081
  • Description: Honouring the dead is upheld in religions and cultures across the world and embedded in rituals and rites of passage that must be adhered to. In many instances, religio-cultural beliefs synthesise or overlap, creating a mutual influence on the person. These religio-cultural rituals are symbolic and important for the living, but they are also equally important for the dead in order to ensure that their souls are returned to the source of their belief system. The COVID-19 lockdown restrictions in South Africa altered death ceremonies and rituals by limiting social gatherings to 50 people to ensure safety protocols like mask wearing and physical distancing. Additionally, family members were prohibited from interacting with the deceased’s body, including performing rituals like washing and dressing, to prevent virus spread. The resultant psychological consequences of these changes to burial practices have not been fully explored in the South African context, and given the importance rituals play, it becomes important to understand them. Studying the psychological consequences of the changes provided insights into individuals’ grief and loss coping in the absence of traditional burial rituals and practices. This is aimed at assisting with the development of interventions for mental health and well-being in future crises. The study explored the psychological consequences of COVID-19 lockdown restrictions on AmaXhosa burial practices and rituals in the Eastern Cape Province of South Africa. AmaXhosa have a rich cultural heritage that places great significance on death and burial rituals. These rituals serve as a way to honour and remember the deceased, provide closure for the bereaved, and maintain social cohesion within the community. The absence of these rituals during the COVID-19 lockdown had consequences for the AmaXhosa’s psychological and religio-cultural well-being. The exploration of their experiences was thus important because it shed light on the importance of these rituals and will help devise innovative ways to address their psychological needs during times of loss. The study utilised a qualitative method, conducting interviews with AmaXhosa aged 18 and older. Participants , Thesis (PhD) -- Faculty of Health Science, 2023
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  • Date Issued: 2023-12

Insights into the relative abundance, demographics and behaviour of sea turtles along the east coast of South Africa

  • Authors: dos Santos, Natalie Aida
  • Date: 2023-12
  • Subjects: Sea turtles -- Conservation -- South Africa , Sea turtles -- South Africa , Photogrammetry
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62495 , vital:72778
  • Description: Sea turtles are ecologically important species that have endured a history of overexploitation. Combined with their long, complex life cycles and ongoing anthropogenic pressures, they remain conservation-dependent. Conservation efforts worldwide focus on sea turtle nesting beach protection, however, comprehensive management strategies require an understanding of their lives at sea which are often spent in distant waters. This understanding is hindered by the relative challenges of in-water studies. South Africa has also focused research and conservation efforts on nesting adult female loggerheads (Caretta caretta) and leatherbacks (Dermochelys coriacea), largely overlooking the species or age classes remaining at sea. Green turtles (Chelonia mydas), hawksbills (Eretmochelys imbricata) and non-breeding loggerheads are frequently stranded and rehabilitated by aquaria, and observed by recreational divers on reefs, suggesting year-round residency along the country’s entire seaboard. Recent technological advancements in photo-identification methods for sea turtles, underwater photography and internet connectivity allowing even recreational divers to become citizen scientists now place us on the verge of an exciting opportunity to unravel the cryptic in-water ecology of these species. The overall aim of this study was to conduct the first assessment of relative abundance, demographics, distribution, and behaviour of sea turtles on reefs within the iSimangaliso and Aliwal Shoal Marine Protected Areas (MPAs) on the east coast of South Africa. A robust photo-identification database was established from research dives, voluntourism and citizen science contributions over a two-year period. From 1057 sightings in the two MPAs, a total of 227 individual sea turtles were identified constituting three species (green turtles: n = 120; hawksbills: n = 46; loggerheads: n = 61). Their relative abundance was proportional to the overall sizes of the South West Indian Ocean (SWIO) populations (green turtles > hawksbills > loggerheads > leatherbacks). However, sightings per unit effort (SPUE) showed that loggerheads were more prevalent than hawksbills in iSimangaliso, likely due to its proximity to this loggerhead rookery. Reefs supported mixed size aggregations with juvenile to adult-sized green turtles (straight carapace lengths, SCLs of 44.9–99.2 cm), whilst hawksbills were mostly juveniles or subadults (SCL range: 37.4–73.4 cm) and loggerheads were mostly adult-sized (SCL range: 66.9–81 cm). Adult sex ratios were slightly female-biased but not significantly different from 1:1. The longest minimum residence periods recorded for individual subadult green and hawksbill turtles (676 and 675 days respectively) and adult-sized loggerheads (621 days) were suggestive of residency. Resident sea turtle activities were typical; they wereobserved foraging, resting, cleaning and interacting year-round, whilst only loggerheads and leatherbacks were observed in breeding behaviour (e.g., patrolling and mating) just after the seasonal arrival of transient individuals. This study thus reveals that the east coast of South Africa hosts regionally important resident and transient sea turtle aggregations with some of the highest SPUE results recorded for the region. This first attempt at monitoring foraging grounds using a variety of techniques and stakeholder groups can serve as the baseline assessment for future work and the foundation for a long-term in-water monitoring programme, allowing for expansion along the entire coastline. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
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  • Date Issued: 2023-12

Integrating state-space modelling and systematic conservation planning to recommend penguin-fishery management zones in Algoa Bay, South Africa

  • Authors: Stockdale, Victoria , Goodall, Victoria , Clifford-Holmes, Jia
  • Date: 2023-12
  • Subjects: Biodiversity conservation -- South Africa -- Port Elizabeth , Natural resource conservation , Marine ecology
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62611 , vital:72828
  • Description: Direct anthropogenic impacts on vulnerable marine ecosystems are growing and require effective conservation measures. Integrating the cost impacts of conservation interventions and optimising the spatiotemporal scale of management interventions may improve cohesion between opposing marine users. This study aimed to integrate spatial and temporal models to advise dynamic penguin-fishery management plans in Algoa Bay, off Gqeberha, South Africa, that would maximise penguin foraging success while minimising the impact on the fishing industry. Determining the foraging locations important to breeding penguins is fundamental when understanding the relationship between fisheries and prey availability. Using Global Positioning System (GPS) data gathered from feeding trips of breeding African penguins between 2012 and 2017, and a hidden Markov model, three behavioural states (foraging, commuting, transitioning) were inferred and used as conservation targets in spatial prioritisation using the prioritizr package in R. The cost to the fishery was estimated using the catch of pelagic fish by the fishing industry between 2012 and 2015 as the proxy. The energetic cost to penguins was the distance they travelled from their breeding colony. Nine prioritisation scenarios, varying in levels of conservation for penguins, spatial cohesion and costs to fisheries, were developed using all year’s combined data to explore an ‘optimal’ closure configuration. The optimal closure was determined as an area which met the targets for penguin conservation, with a low cost to the fishery, close to the breeding colony and spatially clumped. The best solution from each scenario was isolated and the solution for the conservation target of 20% foraging habitat, 10% commuting habitat and 10% transitioning habitat and a boundary penalty of 0.01 (a dimensionless value that is used to reduce spatial fragmentation – increasing the value of the parameter signals that it is important to minimise the total exposed boundary of the prioritisation by favouring solutions where units are clumped together) was chosen as the optimal closure. This configuration was used as a static closure and applied to individual fishing seasons to estimate how the cost of a static closure design to the fishery would vary between seasons. Subsequently, a dynamic closure was designed using the same optimal configuration criteria every year, and the cost to the fishing industry was compared seasonally with the cost of a static closure. The cost to fisheries of dynamic closures was consistently lower than that of a static closure and the area required to meet the penguin feeding targets was drastically reduced. While dynamic closure scenarios show promise to minimise costs to the industry while meeting penguin conservation targets, these configurations were designed retrospectively with data collected after the end of the fishery seasons. For efficient management, the closures need to be designed by using data collected at an appropriate temporal scale. For that, the critical missing dimension to this study is the addition of real-time data on pelagic fish positions, areas of fishing intensity and sites of penguin foraging to create appropriate recommendations for up-to-date fishery exclusion zones. Therefore, the priority now is to explore methods of collecting real-time data on pelagic fish abundance, feeding behaviour of penguins at sea, site and mass of pelagic fishery catch, and using these data to delineate real-time, dynamic closures, coupled with a governance and management strategy that can implement dynamic closures. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
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  • Date Issued: 2023-12

Investigating the effect of peptide-functionalized gold nanoparticles on colon cancer cells

  • Authors: Ramagoma, Rolivhuwa Bishop
  • Date: 2023-12
  • Subjects: Colon (Anatomy) -- Cancer -- Research , Colon (Anatomy) -- Cancer -- Treatment , Nanoparticles
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62564 , vital:72824
  • Description: Colon cancer like any other cancer is a condition in which cells grow uncontrollably and may even spread to other regions of the body through metastasis. Colon cancer was ranked the second leading cause of cancer related deaths worldwide in 2018. Research to advance treatment of cancer keeps advancing daily, However, a big challenge is drug-induced side effects due to toxicity against normal body cells. Therefore, the development of controlled release technologies in conjunction with targeted drug delivery may provide a more efficient and less dangerous approach to overcome the limits of traditional chemotherapy. Including the creation of nanoscale delivery vehicles capable of directing the release of chemotherapeutic drugs into cancer cells only. This study aims to investigate p14 peptide that would specifically target colorectal cancer cells and not normal body cells to develop a targeted drug delivery system using gold nanoparticles. This study serves as a pilot study of the primary aim. To achieve this, the effect of the peptide p14 and peptide functionalized gold nanoparticles (p14-AuNP) on colon cancer cells (HT-29) and normal epithelial cells (KMST-6) was determined. Firstly, gold nanoparticles were chemically synthesised and then functionalized with p14 peptide through Polyethylene glycol. Then assessment of their effect through in vitro cytotoxicity assay (MTT) and gene expression analysis (RT-qPCR) was conducted. Nanoparticles’ synthesis and functionalization was performed and confirmed: In vitro cytotoxicity through MTT assay was successfully conducted and p14-AuNP showed toxicity against colon cancer cells and lesser toxicity towards normal cells as compared to 5-Flourouracil (commercially approved drug for colon cancer treatment). Gene expression analysis revealed that apoptosis was induced in both cell lines by p14-AuNP either through upregulation of caspase 3, 7 and/or BCL2. A cell survival gene, AKT1, also had significant effect on this. CDC42 was downregulated which indicates that cell proliferation was inhibited. , Thesis (MSc) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2023
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  • Date Issued: 2023-12

Investigating the interannual variability of the Benguela coastal upwelling system using a ROMS numerical model from 1993-2014

  • Authors: Silima, Rudzani Faith , Roagosha, Moagabo
  • Date: 2023-12
  • Subjects: Oceanography , Coasts , Marine sciences
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62586 , vital:72826
  • Description: The seasonal and interannual variability of the coastal Benguela upwelling system is described using 22 years (September 1993 - December 2014) of a regional Ocean model (ROMS) output. The model-derived SSTs are evaluated against satellite SSTs (GHRSST) and biases between the datasets investigated, allowing for confidence in using the ROMS data to investigate details of spatial and temporal variability over the Benguela System. Variability of coastal SSTs are investigated together with alongshore winds (from the ERA-Interim Reanalysis product). The driver of the seasonality in upwelling in the Benguela region is the seasonal shift of the South Atlantic High pressure system, leading to an enhanced seasonal signal in the southern Benguela (with strongest upwelling favourable winds in austral spring and summer). In the northern Benguela, the seasonal signal reduces with the peak of upwelling favourable winds shifting to late winter and spring. Interannual variability is evident in the model and is investigated with respect to various modes of climate variability. Though the direct relationship of the Benguela upwelling system to large-scale climate modes such as SAM and ENSO is not clear in most places, some evidence was found for their connection. For instance, in the southernmost cell of the southern Benguela a significant positive and negative correlation exists between SST and wind respectively with ENSO during summer months. This relates to increased (decreased) winds and decreased (increased) SST during La Niña (El Niño) events. Although there was no direct correlation with either SST or alongshore wind anywhere in the Benguela system, there was some indication of its importance. A trend toward a positive SAM (i.e a more poleward position of the South Atlantic High-Pressure system) was consistent with a trend toward enhanced winds and reduced SSTs in the southernmost upwelling cell. In addition to this, there was evidence to suggest that when SAM and ENSO are out-of-phase, which occurred primarily during summer months, the wind and SST response is amplified. This seemed to be particularly true in the first 10 years of the timeseries when the anomalies were strongly alternating. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2023
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  • Date Issued: 2023-12

Isadora duncan: An instrumental case study

  • Authors: Volker,Lauren
  • Date: 2023-12
  • Subjects: Analytical Psychology , Psychology of women
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/63038 , vital:73084
  • Description: The study is an instrumental case study of Isadora Duncan (1877 - 1927). She was a pioneer of modern dance as she developed her unique style of dance that was free-flowing and natural. She further developed her own dance philosophy, which drew the attention of many as its archetypal dancer was a woman who embodied a space that was equal and free. She was not only revolutionary in her unique dance style but also for her strong beliefs. Isadora Duncan believed in anti-marriage, bearing children out of wedlock, and fighting for the emancipation of women where she used dance, her philosophy and post-performance lectures to communicate her thoughts and beliefs. An Instrumental case study is a qualitative method of enquiry, which entails researching an event or individual as a means of providing or gaining insight into a particular phenomenon. Similar to that of a psychobiography, the individual subject can be explored through the lens of a psychological theory for the purpose of creating a coherent psychological case study. The psychoanalytical lens of Carl Jung was used to explore and describe certain aspects of the life and work of Isadora Duncan. The subfield of the Psychology of Women provides a contextual description for the challenges that women faced in the past and in the present, and additionally presents the primary aim of the proposed study. Isadora Duncan was chosen due to her unique dance movement, both literally and figuratively as she developed her ability in giving voice to those emotions that women often kept hidden. She lived during significant times which included radical transformations that brought about noticeable change politically, socially, and culturally. Despite the reformative motions, it did not dismiss its own challenges especially for women. Women faced a constant struggle for freedom from oppression, community rights and gender equality, and Isadora Duncan was no exception to these challenges. Extensive biographical information data was collected and processed into salient features. The study integrates the theory of Carl Jung, the life and dance philosophy of Isadora Duncan and the v challenges faced by women over the centuries and demonstrates the value of using an instrumental case study as a method of inquiry into the exploration of a unique individual. The Psychology of Women provides an understanding of how those past challenges may still be modern-day concerns for the psychology of women today. The findings of the study demonstrated that Isadora Duncan was a unique and innovate women who pushed the boundaries socially. She was determined to perform her unique dance style in times that required women to conform to the cultural expectations. However, in times of trouble and despair, she was able to turn to dance as the individual and collective voice. The contribution of the study rests with its demonstration of the value of a holistic and reflexive exploration of a unique individual woman. It demonstrates the value and application of Jung’s in-depth theoretical understanding of the archetypes in human behaviour and the use of dance as a healing process for psychologically wounded women. Therefore, specific reference to the true inner voice of women allows insight into how clinicians might assist female clients who struggle to release their true inner voice. Furthermore, the study contributes to building knowledge of the challenges that women face in society and allows for future research of exploring integrative theoretical frameworks in understanding the healing of psychologically fragmented women in the 21st century. , Thesis (Ma) -- Faculty of Health Science, 2023
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  • Date Issued: 2023-12

Knowledge, attitudes and practices of municipal workers regarding occupational health and safety in the garden route district municipality, Western Cape

  • Authors: Shoto, Khanyisa
  • Date: 2023-12
  • Subjects: Occupational health and safety , Personal protective equipment
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62991 , vital:73080
  • Description: Background: Workers accidents at work have been demonstrated to be increasingly common and expensive, particularly in developing nations like South Africa. The failure of employers and employees to support and comply to (OHS) regulations measures frequently leads to injuries and accidents that are extremely expensive for both the employer and employees. Regardless of South African legislations that aim to prevent accidents and injuries, scourging statistics of industrial accidents are still a characteristic of OHS. Objectives: This study investigates knowledge gaps, attitude, and the practice of municipal workers regarding OHS at Garden Route District Municipality (GRDM) in the Western Cape Province of South Africa. Methodology: Quantitative approach was taken, and questionnaires were administered to 188 workers employed by the GRDM. A probability sampling method of stratified sampling method was utilised. Results: This study concluded that the workers of the GRDM are knowledgeable about OHS in their workplaces and that municipal workers positively perceive OHS issues in their workplaces. The main reason for this positive perception is because of the management support that the workers receive from the GRDM with regards to OHS matters. Furthermore most workers positively perceived OHS protocols and measures undertaken at the GRDM. Statistical tests proved that the municipal workers who positively perceived OHS protocols and measures were significantly higher (p< 0.05) than those that negatively perceived OHS protocols. Conclusion: The study revealed that GRDM workers were knowledgeable on OHS protocols. This was because of the support that they get from their employer as well as personal health and safety concerns. They positively perceived the OSH measures that are put in place to prevent accidents and injuries. It is only an insignificant percentage of individuals that disagreed with some of the OHS measures and protocols. This study contributes immeasurably to academic research in the field of OHS and extends knowledge that relates workers’ perceptions, safety awareness, as well as workers’ adherence to safety and behaviour. , Thesis (Ma) -- Faculty of Health Science, 2023
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  • Date Issued: 2023-12

Knowledge, attitudes and practices regarding social media as a teaching strategy at a public nursing college in Limpopo province

  • Authors: Halala, Pfukani Symantha
  • Date: 2023-12
  • Subjects: Nurse educators , Teaching strategy
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62849 , vital:73057
  • Description: The emergence of social media (SM) in the academic arena has demonstrated the potential for innovation in teaching and learning. Moreover, its appeal is enhanced by its accessibility and affordability. Consequently, the use of SM has increased globally and has influenced interaction and socialisation amongst educators and learners. In addition, SM has affected learning and how educators teach and collaborate with learners when constructing knowledge in higher education settings. However, despite its advantages, nurse educators' use of SM is less than optimal in teaching. Therefore, the study aimed to explore nurse educators' knowledge, attitudes and practices regarding the use of SM as a teaching strategy at a public nursing college in the province of Limpopo, South Africa. The research design employed for this study was quantitative, explorative, descriptive and contextual, and Roger’s diffusion of innovation theory underpinned the study. Stratified, proportionate sampling was used to select the nurse educators who were respondents to the study’s survey via a questionnaire as the data collection instrument. An educator as a specialist in the use of SM, a statistician and the study supervisors reviewed the questionnaire. A pilot study was conducted to check the validity and reliability of the data collection instrument. Data gathered through the survey was statistically analysed to produce descriptive statistics. The researcher adhered to the ethical principles of respect, beneficence, and justice. The research results indicated that the nurse educators in the study were knowledgeable about SM platforms. Moreover, although they had a positive attitude towards incorporating SM as a teaching strategy. Therefore, based on the research results, recommendations were to facilitate the use of SM as a teaching strategy. , Thesis (Ma) -- Faculty of Health Science, 2023
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  • Date Issued: 2023-12

Mechanical recyclability and biodegradation of biopolymers, biopolymer blends and biocomposite in natural environmental conditions

  • Authors: Nomadolo, Nomvuyo Elizabeth
  • Date: 2023-12
  • Subjects: Polymers , Polymeric composites , Biopolymers
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62539 , vital:72822
  • Description: The present research aimed at investigating mechanical recyclability and studying the potential biotic and abiotic degradation behaviors of biobased biodegradable polymers in different environmental conditions. The mechanical recyclability tests monitored the effect of multiple reprocessing on the mechanical, thermal, physical, chemical, and morphological properties of poly (butylene adipate-co-terephthalate) (PBAT), poly (butylene succinate) (PBS), poly (lactic acid) (PLA), PBAT-PBS blend, and PBAT-thermoplastic starch (TPS) composite. Low-density polyethylene (LDPE), a conventional non-biodegradable plastic, was also monitored for comparison studies. The mechanical recyclability tests were carried out by eight melt extrusion cycles using twin-screw extrusion and injection molding processing techniques. Tensile testing, impact analysis, melt flow index test (MFI), differential scanning calorimetry (DSC), thermogravimetry (TGA), dynamic mechanical analysis (DMA), Fourier Transform Infrared Spectroscopy (FTIR) and scanning electron microscopy (SEM) techniques were employed to monitor the effect mechanical recycling at each melt extrusion cycle. Tensile and impact strength results showed that PBAT and PBAT-TPS biocomposite were mechanical recyclable for at least eight cycles and this was comparable to LDPE recyclability performance. In contrast, neat PBS, PLA, and PBAT-PBS blend were found to be melt extrudable only up to six cycles as the mechanical properties declined with the increase of reprocessing cycles. MFI tests suggest that molecular weight of PBAT and PBAT-TPS were not significantly affected by multiple extrusion cycles while the melt flow properties of PBS, PLA, and PBAT-PBS samples were affected from third cycle. DSC, TGA, and DMA demonstrated that PBAT and PBAT-TPS were more thermo-mechanically stable than PBS, PLA, and PBAT-PBS blend. FTIR spectroscopy results showed that the chemical structure of both PBAT and PBAT-TPS were unaffected by the multiple recycling cycles typically indicated by characteristic peak vibrations bands of C=O and C-O around 1710 cm-1 and 1046-1100 cm-1, respectively. SEM micrographs of PBS, PLA, and PBAT-PBS clearly evidenced the degradation of the biopolymers by severely fractured morphology as a result multiple reprocessing cycle.The rate of aerobic biodegradation for PBAT-PBS and PBAT-PLA blends was examined under controlled home and industrial composting using the CO2 evolution respirometric method. FTIR, DSC, TGA, X-ray diffraction analysis (XRD), and SEM were employed to monitor the changes in the structural, chemical, thermal, and morphological characteristics of the biopolymer blends before and after biodegradation. The biodegradation tests showed that PBAT-PBS and PBAT-PLA blends exhibited higher degradation rates under industrial composting conditions than under home composting conditions. The increased intensity of hydroxyl and carbonyl absorption bands on the FTIR spectra confirmed that the biodegradation process occurred. SEM revealed that there was microbial colony formation and disintegration on the surfaces of the biopolymer blends. Moreover, abiotic degradation results suggested that thermal and hydrolytic conditions influence the degradation process than sunlight exposure. Additionally, aquatic biodegradation results showed that PBAT-PBS blend and PBAT-TPS composite undergo a higher rate of biodegradation as compared to PBAT, PBS, and PLA.The results obtained from this research work conclude that biobased biodegradable polymers can be mechanically recycled, and they are suitable for biological degradation in industrial composting, home composting and marine environment. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2023
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  • Date Issued: 2023-12

Psychobiographical study of Daisy De Melker

  • Authors: Moodley, Kerisha
  • Date: 2023-12
  • Subjects: Psychobiographical study , Antisocial , Personality dynamics
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10948/62913 , vital:73067
  • Description: Daisy Louisa C. de Melker, born on 1 June 1886, was a qualified nurse who is famously known to have poisoned two husbands with strychnine in order to receive life insurance money while living in Turffontein, Johannesburg. Later on, she poisoned her only son with arsenic for reasons which are still unclear, and he passed away soon after. She is notoriously known in history to be the second woman to have been hanged in South Africa due to receiving the death penalty. The primary aim of the study is to explore the life of de Melker to gain an understanding of her personality development, with the aim of acquiring insight into the motivation behind her behaviour. The secondary aim includes exploring apparent Cluster B personality traits. The study is exploratory-descriptive in nature and the subject was chosen through purposive sampling. The study uses a qualitative, psycho-biographical, single-case research design to study De Melker, in order to explore and describe the personality development of her from the theoretical perspectives of Kernberg’s Object Relations Theory. This specific theory was chosen as it allowed the researcher to explore Daisy de Melker’s early childhood and upbringing that impacted the formation of ‘internal representations’ of herself and other people. The theory suggests that when there is a lack of integration of object relations, which is made up of internal and external representations, it may result in the development of a personality disorder, as it places a focus on relationships being more crucial to personality development than individual drives and abilities. Data was collected by triangulating multiple sources of information. Miles and Huberman’s (1994) strategy were used to analyse the data according to three steps, which include data reduction, data display, and conclusion drawing and verification. The significance of doing a psychobiography study lies in merging of personality psychology and historical evidence which represents a growing field in the realm of biography and provides insight into personality development through a theoretical framework. , Thesis (Ma) -- Faculty of Health Science. 2023
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  • Date Issued: 2023-12
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