Analysis of bacterial Mur amide ligase enzymes for the identification of inhibitory compounds by in silico methods
- Authors: Chamboko, Chiratidzo Respina
- Date: 2020
- Subjects: Pathogenic microorganisms -- Analysis , Drug resistance in microorganisms , Microorganisms -- Effect of drugs on , Antibiotics -- Effectiveness , Pathogenic bacteria , Drug tolerance , Enzymes -- Analysis , Peptide antibiotics
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/161911 , vital:40690
- Description: An increased emergence of resistant pathogenic bacterial strains over the years has resulted in many people dying of untreatable infections. This has become one of the most critical global public health problems, as resistant strains are complicating treatment of infectious diseases, increasing human morbidity, mortality, and health care costs. A very limited amount of effective antibiotics is currently available, but the development of novel classes of antibacterial agents is becoming a priority. Mur amide ligases are enzymes that have been identified as potentially good targets for antibiotics, as they are uniquely found in bacteria. They are responsible for the formation of peptide bonds in a growing peptidoglycan structure for bacterial cell walls. The current work presented here focused on characterizing these Mur amide ligase enzymes and obtaining inhibitory compounds that could potentially be of use in drug discovery of antibacterial agents. To do this, multiple sequence alignment, motif analysis and phylogenetic tree constructions were carried out, followed by docking studies and molecular dynamic simulations. Prior to docking, homology modelling of missing residues in the MurF structure (PDB 1GG4) was performed. Characterization results revealed the Mur amide ligase enzymes contained defined conservation in limited regions, that ultimately mapped towards the central domain responsible for ATP binding (presence of a conserved GKT motif). Further analysis of results further unraveled the unique patterns observed within each group of the family of enzymes. As a result of these findings, docking studies were carried out on each Mur amide ligase structure. At most, two ligands were identified to be sufficiently inhibiting each Mur amide ligase. The ligands obtained were SANC00574 and SANC00575 for MurC, SANC00290 and SANC00438 for MurD, SANC00290 and SANC00525 for MurE and SANC00290 and SANC00434 for MurF. The two best ligands identified for each enzyme had docked in the active site of their respective proteins, passed Lipinski’s rule of five and had substantially low binding energies. Molecular dynamic simulations were then performed to analyze the behavior of the proteins and protein-ligand complexes, to confirm the lead compounds as good inhibitors of the Mur amide ligases. In the case of MurC, MurD and MurE complexes, the identified ligands clearly impacted the behavior of the protein, as the ligand bound proteins became more compact and stable, while flexibility decreased. There was however an opposite effect on MurF complexes, that resulted in identified inhibitors being discarded. As a potential next step, in vivo and in vitro experiments can be performed with identified ligands from this research, to further support the information presented.
- Full Text:
- Date Issued: 2020
Analysis of maximum power point tracking data for obtaining photovoltaic parameters
- Authors: Chitura, Anesu Godfrey
- Date: 2020
- Subjects: Photovoltaic power system
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/18576 , vital:42610
- Description: As a standard, photovoltaic (PV) modules are rated by the use of standard test conditions (STC). Such details entail current and voltage (I-V) measurements for modules under an irradiance of 1000 W/m2 an air mass ration of 1.5 global spectrum and 25˚C cell temperature. Outdoor weather conditions continuously vary with time and from one location to the other. This further offset the expected operational power outputs as outdoor conditions are generally characterized by high cell temperatures. The technology one uses will also complicate the power output prediction since different module technologies respond to these outdoor conditions differently. I-V tracers are able to measure the full I-V curve of the module thus can give the operational PV parameters at any given time. However, these tracers are sold at exorbitant prices and they require skilled personnel in order to operate them. Most if not all tracers require isolation of the module under test thereby disrupting the power production function of the module._________________________________________________________________________________________________________________________________________ In this study a method to obtain photovoltaic (PV) parameters using the maximum power point tracking (MPPT) data is presented and tested under natural outdoor conditions. The method features a customized data acquisition system (DAS) designed for the measurement and storage of meteorological and MPPT data. The DAS is capable of extracting parameters from any combination of modules with an open circuit voltage 〖(V〗_oc) less than or equal to 120 Volts and a short circuit current 〖(I〗_sc) of 100 Amps. The system used is capable of extracting MPPT data using a reliable, improved storage and a programmable data logger. In order to match the sampling operational speeds of internal charge controller switches a computer interfaced Peripheral Component Interconnect (PCI) card was also used. Data collection and characterization of the MPPT data was done in such a way that the power generation process remained uninterrupted throughout the whole process. The regression nonlinear least squares method was used to fit MPPT crests and obtain the knee part of the I-V curve. This was then extrapolated to obtain the full I-V curve, which then produces the operational PV parameters. The resultant parameters from the characterization process were logged and accessible at any time. The end result was a method that can be incorporated within a charge controller for quick, hands-free PV parameter extraction, using only the MPPT scanned data.
- Full Text:
- Date Issued: 2020
Analysis of the contribution of socio-economic factors on poor academic performance of grade 12 learners: the case of Butterworth education district, Eastern Cape Province South Africa
- Authors: Lubisi, Anathi
- Date: 2020
- Subjects: Performance standards -- South Africa Teacher effectiveness -- South Africa
- Language: English
- Type: Thesis , Masters , MSoc (Sociology)
- Identifier: http://hdl.handle.net/10353/18141 , vital:42236
- Description: The drive of this research project was to discover factors contributing to the poor academic performance of Grade 12 learners at two selected schools in the Butterworth Education District, Eastern Cape province South Africa. The participants were Grade 12 learners, Grade 12 teachers and parents of Grade 12 learners in both schools. Both schools are located in rural areas of Mnquma municipality under the Butterworth education district. The study used both qualitative and quantitative research methods. The motivation behind using triangulation was to decrease or offset the deficiency of a specific methodology and for deeper understanding of the discoveries of the study. Use of triangulation in this study was aimed at expanding the legitimacy, dependability and generalizability of the results of the study. The findings of the study revealed that the most determining factor that contributes to the poor academic performance of Grade 12 learners in the Butterworth Education District is the unavailability and inadequacy of instructional resources. This is the most critical school resource which the parents cannot afford to buy for their children. Both teachers and Grade 12 learners indicated that they do not have a school library or a community library. The majority of learners also indicated that they do not have enough textbooks in all subjects and their parents concurred that their children end up sharing textbooks with their peers. Furthermore, the three groups of participants agreed that learners do not have access to computers, and the school does not have a science lab for Grade 12 learners doing science. The core argument of this study is that a poor socio-economic background is a primary contributor to low academic performance of Grade 12 learners at the Butterworth Education District. The findings of this study support this thesis as they indicate that instructional resources are inadequate, a school or community library is vii non-existent, and there is not enough textbooks at the selected schools. With parents from low socio-economic background, the Butterworth community is also not in a position to supplement these insufficient instructional resources. This study utilized the cultural reproduction theory of Bourdieu (1977) to explore a system of education in industrialized communities and the way it works to illuminate class differences in academic performance and achievement of Grade 12 learners. The study made recommendations that should be put in place to improve the academic performance of Grade 12 learners in the Butterworth Education District. The most important of these recommendations is that government officials should provide educational resources (teaching and learning resources). Furthermore, the researcher suggest that for further research, it would be interesting to find out about learners that perform well, regardless of identified factors that cause other learners to perform poorly to help them improve their academic performance.
- Full Text:
- Date Issued: 2020
Analysis of the effectiveness of Tulbaghia Violacea extracts as devulcanizing agents for synthetic CIS-1,4-polyisoprene vulcanizates
- Authors: Gxakuma, Lutho
- Date: 2020
- Subjects: Plant extracts , Plant products -- South Africa Medicinal plants -- South Africa Plants -- Analysis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/49093 , vital:41600
- Description: Tulbaghia violacea is an indigenous plant commonly known as wild garlic, wilde knoffel in Afrikaans, isihaqa in Zulu or itswele lomlambo in Xhosa. Its leaves and bulbs are widely used as herbal remedies for various ailments and its medicinal uses include fever and colds, asthma, tuberculosis and stomach problems. Like all other members of the Alliacea family, Tulbaghia violacea produces a distinctive garlic-like odour when its leaves or rhizomes are damaged, resulting in the release of cysteine-derived sulphur compounds which include the oil-soluble organo-sulphur compounds and water-soluble cysteine derivatives. Chemically synthesized sulphur containing compounds have been reported to be effective devulcanizing agents and many industries prefer to perform devulcanization using them. Most commonly applied devulcanizing agents include disulphides, thiophenols and their zinc salts, and mercaptans which are mixed with the rubber scrap powder under specific reaction conditions. In this study, instead of performing devulcanization by using industrial synthesized sulphur containing compounds, the effectiveness of the extracts of organo-sulphur containing compounds from Tulbaghia violacea are evaluated as potential devulcanizing agents for synthetic cis-1,4-polyisoprene vulcanizates. This is a new, cheap and greener practice of rubber devulcanization. Diallyl disulphide, which is one of the organo-sulphur containing compounds present in Tulbaghia violacea, is the devulcanizing agent of interest to this study. The organo-sulphur containing compounds were extracted from the bulbs, roots and leaves of the plant using the soxhlet and ultrasonic bath extraction method. The solvent system used in the soxhlet extraction method was 2% of 2-propanol in n-hexane whereas in ultrasonic bath extraction method the solvent system used was 100% ethanol. An essential oil extract was obtained from the plant organs. The yield of the essential oil extracts obtained using the soxhlet extraction method was higher compared to the yield of the essential oil extracts obtained using the ultrasonic bath extraction method. According to the Fourier-Transform Infrared spectroscopy, the organo-sulphur containing compounds were present in the essential oil extracts of the plant. The essential oil extracts that were extracted using the soxhlet extraction method were chosen for further analysis. It was found out that the allyl sulphide and diallyl disulphide have the same retention time from the High Performance Liquid Chromatography (using a normal phase column). The Differential Scanning Calorimeter indicated that the allyl sulphide was not present in the essential oil extracts whereas the diallyl disulphide was present in the essential oil extract of the roots and leaves. The High Performance Liquid Chromatography was used to quantify the presence of the diallyl disulphide in the essential oil extract of roots and leaves. The quantity of diallyl disulphide was 23% in the dry mass of the essential oil extract of roots. In the essential oil extract of leaves the diallyl disulphide was very low concentrated and the High Performance Liquid Chromatography was less sensitive to detect it. According to the Thermogravimetric Analyser, it was found that the essential oil extracts begin to degrade at 120 °C and experience a multistage degradation. The softening temperature of the essential oil extracts was 60 °C from the Simultaneous Differential Scanning Calorimeter-Thermogravimetric Analyser. Conventional vulcanization of synthetic cis-1,4-polyisoprene was prepared with the vulcanization ingredients of zinc oxide, sulphur, stearic acid and N-tert-butyl-2-benzothiazole sulphenamide. The vulcanized synthetic cis-1,4-polyisoprene produced, under the heating temperature of 160 °C, was mixed with the essential oil extracts using the internal mixer at 60 °C and the two-roll mill. The overall torque, tan delta and the total crosslink density were the properties of interest of synthetic cis-1,4-polyisoprene vulcanizate in this study. The devulcanization temperature for the treated synthetic cis-1,4-polyisoprene vulcanizate with the essential oil extracts was optimized using the Dynamic Moving Die Rheometer and Dynamic Rubber Process Analyser. The amount of essential oil extracts at which they are effective devulcanizing agents for synthetic cis-1,4-polyisoprene vulcanizate was also optimized. During optimization a change on the latter mentioned properties of synthetic cis-1,4-polyisoprene vulcanizate was observed, indicating that the essential oil extracts have an effect as potential devulcanizing agents. The essential oil extracts increased the tan delta and reduced the overall torque, and total crosslink density of synthetic cis-1,4-polyisoprene vulcanizate as expected from devulcanizing agents. 200 °C was a preferable devulcanization temperature whereas essential oil extracts were effective as devulcanizing agent at 1.4%. However, the essential oil extracts influenced the overall torque and tan delta of synthetic cis-1,4-polyisoprene vulcanizate during the mixing process in the internal mixer at 60 °C, which was before heating at 200 °C. The essential oil extracts had a higher influence on the overall torque of synthetic cis-1,4-polyisoprene vulcanizate under heating at 200 °C whereas at 60 °C, during the mixing process, they had a higher influence on the tan delta of synthetic cis-1,4-polyisoprene vulcanizate. The essential oil extract of leaves had a higher influence on the overall torque whereas the essential oil extract of bulbs had a higher influence on the tan delta of synthetic cis-1,4-polyisoprene vulcanizate at 200 °C. The essential oil extract of roots shown a greater influence on the overall torque and tan delta of synthetic cis-1,4-polyisoprene vulcanizate at 60 °C during the mixing process. The effect of the essential oil extracts on the reversing heat capacity of synthetic cis-1,4-polyisoprene vulcanizate was also investigated using the Modulated Differential Scanning Calorimetry method. The essential oil extract of bulbs and roots influenced the reversing heat capacity of synthetic cis-1,4-polyisoprene vulcanizate by reducing it whereas the essential oil extract of leaves caused a temperature shift of the reversing heat capacity curve from the Modulated Differential Scanning Calorimeter thermogram of synthetic cis-1,4-polyisoprene vulcanizate. Therefore, the results indicated that the essential oil extracts of Tulbaghia violacea have an effect as an alternative potential devulcanizing agents for conventional vulcanized synthetic cis-1,4-polyisoprene whether the diallyl disulphide compound is present or not in them.
- Full Text:
- Date Issued: 2020
Analysis of the existence of the Phillips curve, Okun’s law and Taylor rule in the Zambian economy
- Authors: Chella, Siame Nampasa
- Date: 2020
- Subjects: Unemployment -- Effect of inflation -- Mathematical models --Zambia
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10948/49783 , vital:41800
- Description: The objective for this study was to explore the relationship between inflation and growth with an understanding of the effects of unemployment on growth. The primary objective of this paper was to investigate the existence of Phillips Curve, Okun’s Law, and Taylor Rule in Zambia on the basis that Zambia is a youthful economy, with a growing population, which is expected to temper with unemployment rates, as an increase in the supply of labour might be too high for the demand for jobs available. To be specific, the study aims at determining the relationship between inflation and growth and seeks to understand what policy measures have been undertaken to curb inflation and reduce unemployment in Zambia. To achieve this, a quantitative research was undertaken using the ARDL cointegration model in conjunction with sensitivity tests to cater for structural changes in the economy with 2007 standing as the break point, as well as paying attention to the fact that different policy regimes employed during the course of the data covered, that being, 1996 to 2017, would affect that rates of unemployment, inflation and growth of the country, across period under review. The study revealed that Zambia faces a Hybrid New Keynesian Phillips Curve during the period under study which is dominated by a mixture of both back-ward and forward-looking aspects of inflation. This specifically, paints the effects of inflation persistence as well future expectations of inflation on growth. Further, a cointegration relationship was established between unemployment and output, while Taylor principles were found not to apply to the Zambian economy, which have proved in other economies to be foundation blocks for good economic growth and as such significant to both fiscal and monetary policy authorities of the economy. The researcher therefore recommends that Zambian policy makers, that is, both the fiscal and the monetary authorities, work together in order to attain minimal and optimal levels of inflation to help achieve a conducive economic environment for the country.
- Full Text:
- Date Issued: 2020
Analysis of the regulation of HSP90α expression upon differentiation of C2C12 cells
- Authors: Holm, Nathan Christopher
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/163318 , vital:41028
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020.
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- Date Issued: 2020
Analysis of the relationship between changes in macroeconomic variables and various sector price indices of JSE
- Authors: Mapanda, Tungamirai Chisvuvo
- Date: 2020
- Subjects: Johannesburg Stock Exchange , Stock price indexes -- South Africa , Interest rates -- South Africa
- Language: English
- Type: text , Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10962/147445 , vital:38637
- Description: Purpose- The purpose of this paper is to analyse the relationship between changes in domestic macroeconomic variables and various indices of the JSE during the full time period, June 1995 to December 2018 and the sub-periods, June 1995 to June 2007 and July 2007 to December 2018. Design/ methodology/ approach- The paper employs the Autoregressive Distributed Lag (ARDL) model approach to cointegration using monthly data from June 1995 to December 2018. Findings- In terms of the long run, the results show that the coincident indicator measure of domestic economic activity is positively and significantly related to the various JSE indices for all study periods. In terms of inflation, the results show no relationship between inflation rate and the various indices for both whole period and June 1995 to June 2007 sub period. However for the July 2007 to December 2018 sub period, JSE All Share Index and JSE Top 40 Index are negatively related. For the real effective exchange rate, only the Consumer Services Index is positively related to the exchange rate in terms of June 1995 to June 2007 sub period. However, JSE All Share Index and JSE Top 40 Index are negatively related to the exchange rate in all study periods. In terms of the short term interest rate, for the whole period, JSE All Share Index, JSE Top 40 Index, Health Care Index and Telecommunications Index are negatively related to interest rate. In terms of the June 1995 to June 2007 sub period, JSE All Share Index and Industrials Index are negatively related to the short term interest rate. For the July 2007 to December 2018 sub period, Telecommunications Index and Technology Index are negatively related. In terms of the short run, the coincident indicator is positively and significantly related to the various JSE indices for all study periods. Inflation is not significantly related to any index in the whole period. In terms of the June 1995 to June 2007 sub period, Industrials Index and Financials Index are positively related to inflation and in the July 2007 to December 2018 sub period, Consumer Goods Index, Health Index and Consumer Services Index are negatively related to the inflation rate. The real effective exchange rate is positively and significantly related to the various JSE indices in the different study periods. In terms of the short term interest rate, for the whole period and the June 1995 to June 2007 sub period only the Technology Index is not significantly and negatively related to the short term interest rate, but for the July 2007 to December 2018 sub period, Top 40 Index, Telecommunications Index and Technology Index are positively related to the interest rate. Only the Financial Index is negatively related to short term interest rates during this sub period. Research Limitations- Not a lot literature was found on the relationship between macroeconomic variables and the various sector indices of the JSE. Most previous work, in the South African context focused just on the JSE All Share Index. Practical Implications- The findings can help investors diversify their portfolios into indices that benefit from expected changes in macroeconomic variables, such as recessions, rising interest rates, rising inflation or a weakening exchange rate. Alternatively, they can hedge themselves against the negative implications of such macroeconomic changes on portfolio performance. In addition, the findings are important for the monetary authorities to better understand the implications of their policy changes on financial markets.
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- Date Issued: 2020
Analytical evaluation of the effect of aggregate variation on Asphalt Mixture Properties
- Authors: Van Eck, Elandre
- Date: 2020
- Subjects: Aggregates (Building materials)
- Language: English
- Type: Thesis , Masters , MEng
- Identifier: http://hdl.handle.net/10948/49639 , vital:41743
- Description: This research study aimed to conduct an analytical evaluation of the effect of aggregate grading on the properties of asphalt mixtures. This was achieved by obtaining asphalt mix designs from approved civil engineering organisations and determining the relationship between the aggregate grading and the corresponding properties of the asphalt mix designs.
- Full Text: false
- Date Issued: 2020
Application of section 197 of the Labour Relations Act in an insourcing context
- Authors: Mahlati, Nomphelo Miliswa
- Date: 2020
- Subjects: Labor laws and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: http://hdl.handle.net/10948/47392 , vital:39974
- Description: The last two decades have been characterised by a phenomenal rise in the outsourcing of services to external service providers. The driver of this trend has been largely economic, since outsourcing offers more competitive price points for the same service at a comparable service level. Beyond the cost saving objective, the rationale for outsourcing has been to drive core strategy. However, with the economic slowdown, declining consumption and production are forcing entrepreneurs to change their way of thinking about the management of companies. Notably, in recent years there has been a small but a growing reversal of this trend where companies that have previously outsourced functions are being brought back in-house. One of the major concerns surrounding these changes of business strategies is the extent to which the employee rights are safeguarded.
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- Date Issued: 2020
Applying blockchain technology to aspects of electronic health records in South Africa: lessons learnt
- Authors: Adlam, Ryno
- Date: 2020
- Subjects: Blockchains (Databases) , Database security Data protection Medical records -- Data processing
- Language: English
- Type: Thesis , Masters , MIT
- Identifier: http://hdl.handle.net/10948/45996 , vital:39405
- Description: The purpose of this study was to explore the applicability of blockchain technology as a viable alternative for the secure storage and distribution of electronic health records in a South African context. The adoption of electronic health records (EHRs) has grown over recent years. Electronic health records (EHRs) can be seen as electronic versions of patients’ medical history. EHRs promise benefits such as improving the quality of care, reducing medical errors, reducing costs, saving time, and enhancing the availability and sharing of medical records. Blockchain, in simple terms, could be seen as a distributed database controlled by a group of individuals. Blockchain technology differs from other distributed ledger technology by bundling unrelated data into blocks that are chained together in a linked-list manner, hence the name blockchain. Blockchain technology strives to provide desirable features, such as decentralization, immutability, audibility, and transparency. EHRs are traditionally constructed with a cloud-based infrastructure to promote the storing and distribution of medical records. These medical records are commonly stored in a centralized architecture, such as a relational database. The centralized architecture employed by EHRs may present a single point of failure. These kinds of failures may lead to data-breaches. The cloud-based infrastructure is effective and efficient from an availability standpoint. The increased availability of electronic health records has brought forth challenges related to the security and privacy of the patient’s medical records. The sensitive nature of EHRs attracts the attention of cyber-criminals. There has been a rise in the number of data breaches related to electronic health records. The traditional infrastructure used by electronic health records can no longer ensure the privacy and security of patient’s medical records. To determine whether blockchain is a viable alternative to these approaches, the main objective of this study was to compile a technical report on the applicability of aspects of blockchain technology to the secure storage and distribution of electronic health records. The study first conducted a literature review to gather background on the current state of electronic health records and blockchain technology. The results of the literature review were used to compile an initial report. Experiments were conducted with various aspects of blockchain technology to build a technical baseline and to ultimately validate the initial report. The insights gained from the experiments served to refine the initial report into a final technical report. The final deliverable of this study was to devise a technical report. The technical report serves as a generalized overview of the applicability of blockchain technology as a secure storage and distribution mechanism for electronic health records. The main topics covered by the technical report to outline the applicability of blockchain technology to EHRs are as follows: authentication, authorization, audit log, storage and transactions. The insights gained from the study illustrate that permissioned blockchain technology can enhance the traditional AAA security scheme employed by traditional EHRs. The AAA security scheme entails the use of certificate-based authentication and attributebased access control for authorization. Audit logs can be stored in a semi-decentralized architecture that can enhance the security and privacy of audit logs. Using blockchain technology for storing electronic health records might not be a viable alternative to traditional EHRs architecture. Blockchain technology violates certain privacy regulations as information is stored in a permanent manner. Furthermore, blockchain technology is not optimized for dealing with large volumes of data. However, blockchain technology could be used to store a cryptographic hash of electronic health records to ensure the integrity of records. Permissioned blockchain technology can enhance the EHRs transaction process by transacting health records in a peer-to-peer infrastructure. In doing so, the above-mentioned AAA security scheme can enhance the security, confidentiality, and integrity of electronic health records shared across organizational bounds.
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- Date Issued: 2020
Applying high-resolution remote sensing to quantify baboon damage at a sub-compartment level in pine stands in the Mpumalanga escarpment region of South Africa
- Authors: Ferreira, Regardt
- Date: 2020
- Subjects: Environmental sciences -- Remote sensing , Geographic information systems Remote sensing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/49082 , vital:41599
- Description: Managing risk in intensively managed monoculture plantation forests is an essential task to ensure sustainable yield and a continuous flow of forest products. However, since risks can be either biotic or abiotic, not all of them have a predictable pattern of spread, which can cause severe losses if management does not have the chance to implement mitigation action. Monitoring the change in forest health is vital as this provides the opportunity for preventative management and quantifies the amount of damage that management has to deal with. To provide this window of opportunity for appropriate action, constant monitoring is required. Until recently, forest health was measured through field surveys which provided adequate data. This procedure, however, is time consuming. Remote sensing has become very popular as a monitoring tool, due to its ability to provide assessment data in a fraction of the time. In this study, baboon damage in plantations along the Mpumalanga escarpment area of South Africa was monitored using remote sensing methods. While there are many methods of forest health monitoring using remote sensing, some approaches are less suitable as they either monitor damage caused at a plantation level, use lower spatial resolution (>10m) datasets or map damage using one available time period. The purpose of this study was first to establish the impact of baboon damage through time, using Sentinel-2 satellite imagery with all vegetation indices available, and the Extreme Gradient Boosting (XGboost) algorithm. The second part focused on analysing the damage at a tree level using PlanetScope imagery using a deep Learning approach. Overall, the study found that the use of Sentinel-2 data and PlanetScope data could accurately distinguish between the varying severity of baboon damage, achieving an accuracy of 95% and 82%. The processing time of the deep learning Artificial Neural Network (ANN) was greatly affected by the number of hidden layers and neurons used. Implementation of techniques used in this study has the potential to improve the accuracy of forest health monitoring in compartment forestry in South Africa.
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- Date Issued: 2020
Applying the substitution augmentation modification redefinition model towards adoption of e-learning usage at Walter Sisulu University
- Authors: Mabona, Teoflax Anele
- Date: 2020
- Subjects: Educational technology -- South Africa Universities and colleges -- Computer-assisted instruction -- South Africa
- Language: English
- Type: Thesis , Masters , MIT
- Identifier: http://hdl.handle.net/10948/49474 , vital:41724
- Description: The adoption of e-learning by universities around the world has grown drastically during this time of the Fourth Industrial Revolution. Most universities implement the full utilisation of e-learning, conducting classes only by using online course delivery. Some universities still prefer a blended learning approach where classes are presented to students using both the traditional way of teaching and online e-learning platforms. Walter Sisulu University (WSU) is one of those universities that uses blended learning. The university started using this approach in 2009, through a partnership between WSU and the Netherlands for learning and teaching enhancement using e-learning. The adoption and usage of e-learning in universities is a challenge, which some researchers have investigated. This study aimed to apply the Substitution; Augmentation; Modification; Redefinition (SAMR) Model to enhance the adoption of e-learning and its usage at WSU. To obtain results, a quantitative method using approaches, such as WiseUp logs, WiseUp e-learning usage publications, WSU e-learning documents and literature, was applied. The study, according to the WiseUp logs, found that the e-learning levels at the university were low. Using the WiseUp e-learning usage publications, revealed that the university was still having some challenges regarding the low usage of WiseUp. Some of the students were not aware of the WiseUp platform as they said the system was only shown to them once and never again for continuous training. Most lecturers’ challenge was that the system was time-consuming to upload online content. This study used the WSU e-learning documents to show that the institution employed some initiatives for the adoption and usage of WiseUp, such as the LTD (Learning Teaching Development) department orientating students about WiseUp, just after their registration. The study ‘s objective was achieved, which was to apply the Substitution; Augmentation; Modification; Redefinition (SAMR) Model to enhance the adoption and usage of e-learning at WSU. The study applied the Substitution; Augmentation; Modification; Redefinition (SAMR) Model to provide a clear understanding of WSU’s position in terms of e-learning adoption and usage. The overall result from the study showed that WSU was based mostly on the first three levels of teaching and learning integration into technology, and in the enhancement level of the Substitution; Augmentation; Modification; Redefinition (SAMR) Model. The researcher noted that much still needed to be done because the usage of the system was still very low but its integration into teaching and learning was positive.
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- Date Issued: 2020
Approaches used by NGOs in managing HIV/AIDS programmes in the Eastern Cape
- Authors: Budaza, Silakhe
- Date: 2020
- Subjects: HIV(Disease)-- Psychological aspects -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/49775 , vital:41799
- Description: The purpose of this research study was to explore the interventions NGOs used in addressing the prevalence of HIV/AIDS along with an exploration of the management and causal factors behind project failures and success. Five districts in the province of the Eastern Cape, South Africa were used in the study. The study had three objectives- the first being to identify the various approaches used to manage HIV/AIDS by NGOs; the second was to consider the influence HIV/AIDS policies on the health sector NGOs and finally the third objective of the study was to explore the monitoring and evaluation mechanism set in place to track progress, or lack of, in HIV/AIDS programs. The research design followed a qualitative methodology and data was gathered from ten participants through semi-structured interviews and supported by a document analysis of existing HIV and AIDS policies from both a national and provincial level which were qualitative. The findings showed that a lack of adequate or delayed funding affected the overall performance of NGOs as well as approaches such as home-based care and educational support programs. Resource mobilization and fundraising ought to be diversified so that there is a committed and varied pool of funds to enable the execution of HIV/AIDS services and programs. The government and other stakeholders should build on local capacity to implement these various programs and build financial, social and organizational sustainability.
- Full Text:
- Date Issued: 2020
Approaches used by NGOs in managing HIV/AIDS programmes in the Eastern Cape
- Authors: Budaza, Silakhe
- Date: 2020
- Subjects: Non-governmental organizations -- South Africa , Disease management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/50457 , vital:42169
- Description: The purpose of this research study was to explore the interventions NGOs used in addressing the prevalence of HIV/AIDS along with an exploration of the management and causal factors behind project failures and success. Five districts in the province of the Eastern Cape, South Africa were used in the study. The study had three objectives- the first being to identify the various approaches used to manage HIV/AIDS by NGOs; the second was to consider the influence HIV/AIDS policies on the health sector NGOs and finally the third objective of the study was to explore the monitoring and evaluation mechanism set in place to track progress, or lack of, in HIV/AIDS programs. The research design followed a qualitative methodology and data was gathered from ten participants through semi-structured interviews and supported by a document analysis of existing HIV and AIDS policies from both a national and provincial level which were qualitative. The findings showed that a lack of adequate or delayed funding affected the overall performance of NGOs as well as approaches such as home-based care and educational support programs. In addition, the negative stigma associated with HIV/AIDS has been a looming issue since 2004 which continues to undermine efforts of NGOs in their means to manage HIV/AIDS. The consequences of stigma from the findings are treatment adherence issues and little to no attendance of HIV/AIDS programs provided by the respective NGOs. Effective planning and management along with capacitated staff and volunteers increased the positive outcome of HIV/AIDS programs and services offered by the NGOs. From the study findings, it is recommended that the planning of HIV/AIDS approaches should consider resource planning to avoid abandoning projects midway due to a lack of resources. The management and monitoring of NGOs within the health sector, dealing with epidemics such as HIV/AIDS need to build on flexibility in implementation and have effective risk assessments in place as a contingency, so that corrective action is taken. Resource mobilization and fundraising ought to be diversified so that there is a committed and varied pool of funds to enable the execution of HIV/AIDS services and programs. The government and other stakeholders should build on local capacity to implement these various programs and also build financial, social and organizational sustainability.
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- Date Issued: 2020
Assessing community structure and trophic interrelationships in three differently impacted headwater streams in the AmatholeWinterberg freshwater ecoregion, South Africa
- Authors: Matomela, Nonjabulo Happy
- Date: 2020
- Subjects: Stream ecology -- South Africa -- Eastern Cape , Freshwater animals -- Ecology -- South Africa -- Eastern Cape , Kat River (South Africa) -- , Lushington River (South Africa) , Elands River (South Africa) , Eyre River (South Africa) , Food chains (Ecology) -- South Africa -- Eastern Cape , Water quality -- South Africa -- Eastern Cape , Land use -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/147653 , vital:38658
- Description: Afromontane regions represent some of the highly threatened ecosystems on the planet as they harbour endemic and often relic freshwater fauna. These ecologically sensitive ecosystems have been altered by multiple impacts, including invasion by non-native fishes, replacement of diverse indigenous vegetation with monoculture plantations, agricultural and mining activities, hydrological modifications, and degradation of instream habitats, with potential detrimental effects on aquatic community structures and food web dynamics. The aim of the present study was to compare spatio-temporal patterns of macroinvertebrate and fish communities as well as food web dynamics in three differently impacted headwater tributaries of the Kat River in the Amathole-Winterberg freshwater ecoregion in the Eastern Cape Province of South Africa. The upper Kat River catchment was the ideal model for purposes of the present study as it contains streams with varying degrees of human impact. The streams considered in the present study were (i) the Eyre River which was considered to closely represent near-natural conditions as it is the least impacted stream in this catchment, with the riparian zone dominated by relatively intact and diverse native woody vegetation, (ii) the Elands River whose catchment has been altered by agricultural activities, and (iii) the Lushington River, whose riparian zone has been heavily invaded by black wattle. The three headwater streams were generally distinguished based on the physical-chemical variables. The Elands River was characterised by high conductivity and total dissolved solids (TDS). In addition, the Elands River was more alkaline and relatively warmer than the other two rivers. This suggested the negative influence of agriculture activities on the water quality in the Elands River. In general, the Lushington and Eyre rivers had comparable physical and chemical variables. However, the Lushington River was generally characterised by low streamflow, likely as a consequent of black wattle which is known for altering hydrological regimes of streams .The Eyre River and Lushington River were comparable in terms of macroinvertebrate richness and diversity, whereas the Elands River was characterised by low macroinvertebrate diversity and richness. Redundancy analysis indicated that the macroinvertebrate communities were mostly influenced by seasonality, with land-use accounting for a small but significant difference in community composition. In comparison, the generalised linear mixed-effects models (GLMMs) showed that chubbyhead barb abundance was significantly influenced by land-use patterns. Evaluation of food web dynamics using stable isotope analysis revealed that the food web structure in the three rivers differed substantially as a result of land-use. Specifically, the Eyre River was characterised by less variation in stable isotope values of basal food sources and consequently, the consumer groups had a narrow isotopic breadth. In contrast, the Elands River was characterised by a wide variation in basal food sources and therefore, a wider isotopic breadth for consumers. The Lushington River was spatially variable in terms of basal sources and isotopic breadth for consumers. The study concluded that food webs and trophic interrelationships were a more informative indicator of land-use than community structure was in evaluating the impact of land-use patterns on aquatic communities. In addition, future studies should seek to investigate food webs interrelationships in addition to community structure to infer a more conclusive river assessment.
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- Date Issued: 2020
Assessing factors that mitigate business risks in the operations of emerging black-owned businesses in Mthatha, Eastern Cape
- Authors: Bovungana, Afrika Khanyiso
- Date: 2020
- Subjects: Business enterprises, Black
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/47502 , vital:40121
- Description: The study sought to assessfactors that mitigate business risks in the operations of emerging black-owned businesses in Mthatha, Eastern Cape. Small businesses in the country are significant contributorsto the South African economy,as they provide employment and development opportunities to disadvantaged communities. Many of these businesses are operationalbut have no formal systems in place to operate effectively. The lack of proper operating procedures can result ininefficiencies that often lead to the collapse and eventual failure of these businesses. Van Vuuren,a professor at the University of Pretoria,mentionedin a round table dialogue that in South Africa the early stage entrepreneurial activity rate has been recorded as9.2% whereas in similar developing economies such as Brazil early stage entrepreneurialactivity isapproximately 40%. The study utilised a quantitative research design and employed a Likert scale questionnaire on a sample of 64 black-owned small, medium and micro enterprises (SMMEs) chosen by means of a random probability samplingmethod. QuestionPro was used to analyse the data and discuss the findings of the study.The various factors identified and highlighted indicate the integral challenges faced by the black-owned SMMEs remain prevalent. The success or failure is dependent on dynamic interventions that are taken into consideration and implemented for the improvement of the environment and business life cycle of black-owned businesses.
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- Date Issued: 2020
Assessing invasive alien aquatic plant species, phytoremediation effects using biological indicators in the Swartkops River system
- Authors: Tshithukhe, Getrude
- Date: 2020
- Subjects: Aquatic weeds -- Biological control -- South Africa -- Eastern Cape , Alien plants -- Biological control -- South Africa -- Eastern Cape , Phytoremediation -- South Africa
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167665 , vital:41501
- Description: Pollution effluents in freshwater ecosystems are becoming increasingly ubiquitous as a result of cumulative anthropogenic activities, such as wastewater treatments works, and industrial, agricultural and mining activities. These activities are more noticeable in urban river atchments where there is greater human population densities and industrial developments. The ecological effects of anthropogenic activities on freshwater ecosystems include: excessive deposition of contaminants such as nutrients, pharmaceuticals, microplastics and other chemicals, which change physicochemical properties, causing a decline in aquatic biodiversity. These effects, impact negatively on the resilience of freshwater, making the systems vulnerable to invasion by alien aquatic plants. Ultimately, the loss of local biodiversity associated with the invasive alien aquatic plants (IAAP) results in a loss of some ecosystem goods and services. The Swartkops River system, Eastern Cape Province, drains most of the neighbouring formal and informal settlements, agricultural lands and industries, and hence is exposed to water pollution from human activities along the river catchment. Various water quality assessments are needed to evaluate the extent of pollutants and their impacts on the river ecosystem. Phytoremediation is one approach employed internationally for removing harmful nutrients and chemicals in freshwater ecosystems. Most studies measure the success of phytoremediation through measuring the reduction of contaminants in water or soil chemistry in mesocosm settings, which may not take into account all the important environmental factors that exist in the field. The present study assesses the phytoremediation potential of Pontederia (Eichhornia) crassipes and Salvinia molesta by evaluating water and sediment chemistry, periphyton and aquatic macroinvertebrate community recovery along seven field sites (excluding IAAP species mats sites) located upstream and downstream IAAP species mats on the Swartkops River between April and September 2018. Water and sediment samples were collected once monthly on ten seven field sites, including the IAAP species mat sites. Periphyton and aquatic macroinvertebrates were collected on seven sites, excluding the IAAP mat sites. Pontederia crassipes and S. molesta infestation in Swartkops River showed positive phytoremediation results and improved some water and sediment chemistry in the downstream treatments as compared to upstream treatments. Although there were some fluctuations with some variables, important water and sediment chemistry were reduced downstream. By contrast, biological assessment results did not show any response to the presence of IAAP species and phytoremediation. Periphyton and aquatic macroinvertebrates diversity and community assemblages were more influenced by water quality. Although IAAP species did provide improvement in water and sediments chemistry, multiple effluent point and non-point sources in Swartkops outpaced phytoremediation. Taxa evenness and relative taxa abundance showed significant differences between the upstream and downstream sites, however taxa richness and Shannon’s diversity showed no significant differences, indicating no relative recovery in biodiversity for either periphyton or aquatic macroinvertebrates. Similarly, the upstream and downstream sites showed similar periphyton and aquatic macroinvertebrates assemblages structure, all dominated by pollution tolerant taxa, thus indicating no functional diversity improvement down river; because of improvement in water chemistry downstream sites, it was expected that periphyton and aquatic macroinvertebrates assemblage structure would also improve at these downstream sites.It is possible that the phytoremediation process is outpaced by effluent discharges, given the multiple sources and distance between upstream and downstream mat sites. The study showed phytoremediation potential but the results were not indicated by biological indicators. A replica study conducted on a moderately disturbed river system is recommended to measure the success and recovery of biological indicators and assemblage composition following IAAP species phytoremediation; a moderately disturbed river compared to a largely disturbed river will broaden findings and look at differences for a wider application of phytoremediation.
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- Date Issued: 2020
Assessing the implementation of long-acting reversible contraceptive implant, Implanon NXT, roll out in East London, Eastern Cape, South Africa.
- Authors: Mdingi, Mildred Mandisa
- Date: 2020
- Subjects: Primary health care
- Language: English
- Type: Thesis , Masters , MPH
- Identifier: http://hdl.handle.net/10353/18452 , vital:42267
- Description: Background: In 2014, South African Department of Health (SADOH) initiated use of Implanon NXT, a type of implant in addition to long-acting reversible contraceptives available in the country to expand contraceptives options for women of reproductive age. After its introduction, there were reports on early removals and frequent side effects. It is therefore vital to identify how successful the implementation of this method was in particular in East London area. Objectives: The purpose of this study was to evaluate how successful the implementation of Implanon NXT roll out was in East London, Eastern Cape. Additionally, the study also aimed to understand the views health care providers have of the Implanon NXT. Methods: A retrospective crosssectional design using existing data from family planning registers and distribution of questionnaires to Health Care Providers responsible for insertion and removal of the method (HCP). Results: February 2014 to March 2019, 1238 Implanon NXT insertions were done and only 266 removals in one study site. Out of the 266 participants, 67.7% of the participants removed the Implanon for reasons other than ill health (side effects), while the remaining 32.3% had experienced side effects and opted for early Implanon removal. Bleeding was the most common side effect reported by 44 of 266 (16.5%) participants. The health care providers who completed questionnaires for this study were overall confident in providing the Implanon NXT services. Conclusion: Implementation of a new contraceptive method requires clear standardised policies and guidelines on counselling and management of side effects. Findings of this study are reassuring that Implanon NXT had a role to play in prevention of unintended pregnancies. Implementation and rollout in one site reviewed has been demonstrated to be successful. The respondents in this study possesses knowledge about the Implanon NXT.
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- Date Issued: 2020
Assessing the Social Sustainability of Wind Energy in the Karoo
- Authors: Hechter, J P
- Date: 2020
- Subjects: Wind power -- South Africa -- Karoo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/48378 , vital:40854
- Description: Background: In an ecologically conscious world, the need for sustainable development and the need to produce alternative energy is at the forefront of discussions. South Africa has been witnessing to a surge in wind energy developments in recent years. Although, environmental impacts often enjoy more emphasis, social impacts are equally important. Recently social sustainability was seen as an integral part of sustainable development for the future and can no longer be ignored. Wind energy is pathway towards sustainable development but can have detrimental impacts on social sustainability. On the one hand wind energy is said to impact culture, sense of places and cause unrest in a community, however, on the other hand it is said to be socially sustainable to the point where wind energy is incorporated into local culture and not to mention the employment opportunities it can create. This is particularly important for vulnerable communities found in the Karoo, where unemployment is very high. Purpose/ Aim: The aim of this study was to assess the social sustainability of a wind energy project in the Karoo by constructing a theoretical assessment model designed to produce appropriate and specific social indicators. Methodology: A literature review was undertaken to gain insights into the social sustainability of wind energy which also served as the theoretical backbone for the model and assessment parameters. The assessment model produced 10 indicators which was used as interview schedule with key stakeholders in the study area. A total of 31 qualitative interviews were conducted and produced a good data set. Findings: The results from this study show that here was no significant negative impact reported with regards to social sustainability. The wind developer is committed to the community based on the various social initiative funded by it. Although a definite positive impact and improvement in terms of social sustainability is evident in the area, there is still a long way to go as many outcomes set by the wind developer is yet to achieved.
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- Date Issued: 2020
Assessment of the implementation of the citizen centric strategies for social grant service delivery :Chris Hani District
- Authors: Danster, Siyabulela
- Date: 2020
- Subjects: South African Social Security Agency -- South Africa -- Chris Hani District
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/46692 , vital:39609
- Description: This study aimed to assess the implementation of citizen centric strategies for social grant service delivery in the Chris Hani District and if SASSA is still living up to its promise. SASSA claims it will always pay the right social grant, to the right person, at the right time and place. This study aimed to understand and assess the different strategies of SASSA towards enhancing citizen experience in delivering social grants. The study’s objective was to assess citizen experience regarding the SASSA social grant payment systems, the social grant application processes, and SASSA communication and advocacy programmes. To conduct this study, a qualitative research methodology was employed. The population sample comprised of SASSA officials in the Chris Hani District. Semi-structured interviews were conducted with SASSA officials. The findings of this study reveal that SASSA did well to improve application turnaround time from 90 days to one day. Even so, payment methods must be improved. The recommendations suggest that SASSA should consider insourcing the function of paying social grants and consider opening the closed pay-points, as beneficiaries are struggling to travel to the remaining opened pay-points. The findings also proposed aligning the SocPen and MIS.
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- Date Issued: 2020