International trade and environmental disputes : an analysis of Article XX of the General Agreement on Tariffs and Trade (1994) and environmental policies of the developing and developed world
- Authors: Manjoro, Faith Tendayi
- Date: 2007
- Subjects: World Trade Organization General Agreement on Tariffs and Trade (Organization) Environmental protection Environmental degradation Free trade -- Environmental aspects Foreign trade regulation International trade
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:3713 , http://hdl.handle.net/10962/d1007444
- Description: A major problem emanating from the trade/environment conflict is the use of trade measures, such as restrictions and sanctions, as tools for environmental protection. Proponents of free trade argue that the use of these measures is tantamount to abuse of environmental standards for protectionist ends. This is particularly so if the imposition of the standard amounts to a unilateral act which blocks the entry of a specified product into the market of another member state for reasons other than environmental protection. Environmentalists at the same time argue that free trade will lead to environmental degradation and therefore advocate for the use of trade-restrictive measures to safeguard against the destruction of the environment. The GATT has proved problematic when it comes to the resolution of trade/environment conflicts. The GATT aims at trade liberalisation yet most environmental policies are enforced through trade-restrictive devices like quotas and licences. Article XX of the GATT is anomalous: it does not explicitly mention the environment, yet member states rely on it as an environmental protection clause. This thesis discusses the various issues emanating from the trade/environmental debate. The history of Article XX is reviewed and the issues that arise in the adjudication of Articles XX (b) and (g) in a trade/environment context are analysed in light of the decisions by the GATTIWTO dispute settlement bodies. The role played by Multilateral Environmental Agreements (MEAs) in protecting the environment is discussed. However, the relationship between MEAs and the WTO is also scrutinised as these rule-making bodies often come into conflict: firstly, because they serve two differing interests - on the one hand, MEAs allow for the use of trade restrictive measures in environmental agreements and on the other, the WTO calls for unrestricted trade unless exceptional circumstances exist; and secondly, member states that are party to both the WTO and MEAs are often forced to subscribe to international trade rules that are incompatible with those in environmental agreements. The trade/environmental debate is important to both the developed and developing worlds. The developed world is in favour of environmental policies which protect the environment from degradation. On the other hand, the developing world is in desperate need of the benefits of trade liberalisation so as to cater for high unemployment rates and poor economic growth. The question thus arises as to whether, when environmental issues are promoted, developing countries will not suffer at the expense of developed nations which may engage in protectionist measures under the pretext of environmental conservation. The divide between developed and developing countries is illustrated in Chapter 5 through case studies on coal mining in the USA and South Africa. The conclusion reached is that total co-operation is essential between developed and developing states for success in safeguarding the environment from degradation. Accordingly, the trade/environmental debate cannot be isolated from the conflicting approaches in developed and developing countries. The conclusions in the final chapter seek to strike a balance between trade liberalisation and environmental protection. Recommendations are made on how the trade/environmental challenges could be dealt with and the regulation of trade restrictive devices to exclude, or at least limit, protectionism.
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- Date Issued: 2007
Investigating grade 10 geography teachers' implementation of a learner-centred approach in selected Namibian schools
- Authors: Angula, Adelheid
- Date: 2007
- Subjects: Geography -- Study and teaching (Secondary) -- Namibia Student-centered learning -- Namibia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1851 , http://hdl.handle.net/10962/d1004459
- Description: Geography, more than other subjects in the curriculum, plays a central role in general education in equipping learners with skills and competencies needed for modern living and global citizenship. A learner-centred education in Namibia was adopted to provide more opportunities for learners to develop the required skills and competencies irrespective of their different cultural background. This small-scale case study investigated three Grade 10 geography teachers' understanding and implementation ofa learner-centred approach to gain insights into how leamer-centred education is being implemented in the context of Geography. The research design adopted a qualitative approach within an interpretative orientation. Data were collected through interviews, classroom observations and documentary analysis. The research was conducted at three selected schools in Oshikoto Region with three Grade 10 geography teachers The findings revealed, firstly, that teachers have a limited understanding of the key ideas ofLCE, such as, prior knowledge, role of questioning, and social interaction in learning; use of resources and the types of assessment activities which comply with the aims of LCE. Seconclly, the findings revealed that the policy documents, such as syllabuses, that are being used by the participants are not in line with the tenets ofLCE. Thirdly, teachers appeared to have limited subject knowledge, as revealed by their lack of understanding of how to translate the aims and assessment objectives into their daily lessons. The study therefore raises some possibilities for improving the implementation of LCE in the selected schools if the gaps as identified by this study are reduced.
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- Date Issued: 2007
Investigating the effect of Glomus etunicatum colonization on structure and phloem transport in roots of Eragrostis curvula (Umgeni)
- Authors: Skinner, Amy
- Date: 2007
- Subjects: Glomus (Fungi) , Phloem , Plant translocation , Weeping lovegrass , Vesicular-arbuscular mycorrhizas , Mycorrhizal fungi , Mycorrhizas
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4227 , http://hdl.handle.net/10962/d1003796 , Glomus (Fungi) , Phloem , Plant translocation , Weeping lovegrass , Vesicular-arbuscular mycorrhizas , Mycorrhizal fungi , Mycorrhizas
- Description: The symbiotic unit of an arbuscular mycorrhizal fungus and its host is able toachieve and maintain far higher inflow of nutrients than non-mycorrhizal roots. The colonization strategy of the mycobiont within the plant is intrinsic to the symbiosis with respect to both structural adaptations and nutrient exchange. An investigation into the effect of Glomus etunicatum colonization on the structure and phloem transport in Eragrostis curvula (Umgeni) allowed for greater insight into the dynamic of the symbiosis. The combined use of stains (such as Trypan Blue, Chlorazol Black, Safranin and Fast Green), and techniques, (such as freeze-microtome transverse sectioning and permanent slide preparations) contributed to a successful general observation of an intermediate colonization strategy using light microscopy methods. However, clarity into structural detail of mycorrhizal forms required electron microscopy studies. The SEM method used with freeze fracture was a relatively quick and simple method allowing for the observation of surface and internal features. The TEM method allowed for highresolution images providing insight into the variations in the apoplasmic compartmental form, and how this may relate to the function of the symbiosis with regard to fungal coils or arbuscules. The apoplasmic nature of mycorrhizas was substantiated and no symplasmic connections were found between symbionts. Fluorescence studies demonstrated that 5,6-carboxyfluorescein was transported through the phloem into the roots of E. curvula, but remained predominantly in the root phloem. Unloading only occurred in optimal nutrient exchange areas of meristimatic lateral or apical growth regions. It was not possible, using fluorescence techniques and related equipment available, to conclusively establish if there were symplasmic connections between the mycobiont and its host or if bidirectional transfer of nutrients occurred at the same interface.
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- Date Issued: 2007
Investigating the feasibility of an indoor aquatic centre for the Nelson Mandela Bay Metropole
- Authors: Janse van Rensburg, Philippus Jacobus
- Date: 2007
- Subjects: Aquatic sports facilities -- South Africa -- Port Elizabeth -- Designs and plans , Sports facilities -- South Africa -- Port Elizabeth , Recreation centers -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8739 , http://hdl.handle.net/10948/791 , Aquatic sports facilities -- South Africa -- Port Elizabeth -- Designs and plans , Sports facilities -- South Africa -- Port Elizabeth , Recreation centers -- South Africa -- Eastern Cape
- Description: The purpose of this study was to investigate the feasibility of an Indoor Aquatic Centre for the Nelson Mandela Bay Metropole. The main problem was to establish the minimum aquatic facilities an Indoor Aquatic Centre must have, to be able to host National and International aquatic events. The sub problems identified to address the main problem were as follows: - Should accommodation be available at the Indoor Aquatic Centre? - Should medical facilities be available at the Indoor Aquatic Centre? - Should there be a gymnasium at the Indoor Aquatic Centre? - Should there be food malls available at the Indoor Aquatic Centre? - Will sports tourism in the Nelson Mandela Bay Metropole benefit from an Indoor Aquatic Centre? In this study the researcher discusses current tourism opportunities, possible sports tourism opportunities and the current aquatic facilities the Nelson Mandela Bay Metropole has to offer. From the discussion regarding these issues it was found that the metropole do offer many attraction opportunities including the following: - Adventure and Sport; - Agriculture; - Arts and Culture; - Business and Conferencing; - Coastal and Beaches; - Entertainment and Shopping; - Historical; - Wild Life and Nature. Adventure and Sport attractions contribute to sports tourism in the metropole and an Indoor Aquatic Centre could enable the metropole to improve sports tourism figures to the metropole. Sports tourism is defined as any tourism that is linked with a sports event. The conclusion can be made that the main initiative for sports tourism is to host a major sporting event to attract tourists. Adding other tourist attractions makes the destination more attractive. It is also important that the community is educated and informed about sports tourism so that the tourist and the community benefits from the event. Indoor Aquatic Centres found in countries like Australia, Canada, Europe, Russia, The Far East, United Kingdom and the United States of America, were discussed to identify the minimum requirements for an Indoor Aquatic Centre. The literature study has shown that the Indoor Aquatic Centre should be a multi purpose centre with a minimum of two heated pools and leisure facilities included. This will enable the centre to generate different streams of income to enhance sustainability. The aquatic centre should also be designed to host National and International events, but not necessarily for Olympic Games events. An empirical study was done to see if the respondents agree on the minimum requirements for an Indoor Aquatic Centre, identified by the literature study and if they agree that sports tourism within the Nelson Mandela Bay Metropole will benefit from an Indoor Aquatic Centre. The conclusion of the empirical study is that the respondents’ view correlate with the information found during the literature study regarding the minimum requirements for an Indoor Aquatic Centre listed as follows: - The minimum number of heated pools are two consisting of a fifty metre ten lane pool and a twenty five metre ten lane pool; - A food mall that consists of a restaurant, fast food stalls and a food store; - Medical facilities, conference facilities, gymnasium and other leisure facilities must also be available; - Accommodation facilities must be available and consists of two and three bedroom fully serviced apartments; - The Indoor Aquatic centre must be able to have seating for 2000 people and parking for 1000 vehicles. The study has addressed the main problem and the sub problems, but further research needs to be conducted regarding the funding of the Indoor Aquatic Centre as this issue was not successfully addressed. More information is needed regarding the different ways of funding and who the stakeholders should be in this process.
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- Date Issued: 2007
Investigating the implementation of continuous assessment at the lower primary phase in a Namibian school
- Authors: Hamukonda, Paulina
- Date: 2007
- Subjects: Education, Elementary -- Namibia Educational tests and measurements -- Namibia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1592 , http://hdl.handle.net/10962/d1003474
- Description: Many Namibian teachers experience difficulties in actualising the principles of learner-centred education, one of which being continuous assessment. Continuous assessment serves important formative purposes. That is, it can inform education stakeholders and help improve learners’ progress in the teaching and learning environment. Although a great deal is known about continuous assessment in general, not much is yet known about how Namibian lower primary teachers actually understand and implement continuous assessment in the classroom. This study investigates the assessment practices of three Grade Three teachers in a school in Oshana Region of Namibia. The study found that although the teachers who participated in the investigation have a reasonably sound knowledge of continuous assessment in theory, they lack implementation skills. They were unable to effectively transfer what they know to what they actually do. This study found that as much as training of teachers is important, the ongoing professional development of teachers is more appropriate for enhancing a complex understanding of issues related to learner-centred education that will help them to manage continuous assessment more effectively.
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- Date Issued: 2007
Investigating the implementation of continuous assessment by grade 7 Social Studies teachers in the Oshana education region
- Authors: Matheus, Hilya
- Date: 2007
- Subjects: Educational tests and measurements -- Namibia Social sciences -- Study and teaching (Elementary) -- Namibia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1593 , http://hdl.handle.net/10962/d1003475
- Description: Since independence in 1990, Namibia’s Ministry of Education Sport and Culture has focused on curriculum and assessment reforms. The introduction of Continuous Assessment (CA) for appraising learners’ achievement was considered to be a very important aspect of teaching and learning. This study focused on the implementation of CA in schools. The study explores how the selected grade 7 Social Studies teachers understand and implement CA. This study was conducted in the Oshana region of Namibia. Four grade 7 Social Studies teachers participated from two different schools. This study was done through a case study to gain insight into the implementation of CA in schools. Semi-structured interviews, observations and document analysis were the research methods used to collect the data. The findings indicate that these teachers have a theoretical understanding of what CA implies and what role it plays and are aware of assessment strategies. However these theoretical perspectives are not translated into practice. This implies a limited understanding of CA and reflects a gap between the teachers’ theoretical understanding of CA and its practical implementation. The study also identified various systemic factors that hamper the implementation of CA, which include overcrowded classrooms, increased teachers’ workload by CA, shortages of resources such as textbooks and lack of professional development. The nature of pre-service teacher education the participants went through, professional development programmes that are given to teachers as in-service training and a lack of guidelines in the syllabus on how to approach continuous assessment are clearly evident in the findings as factors that contribute to the teachers’ gap between theory and practice. The study concludes that even though a number of workshops on continuous assessment were conducted, the teachers still could not implement it as expected. The study findings inform my professional context as an Advisory Teacher and that of my colleagues. Therefore it made me realize what the teachers’ needs are and what kind of support they require.
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- Date Issued: 2007
Investigating the influences of validation on pharmaceutical manufacturing processes
- Authors: Muller, Damian Christian
- Date: 2007
- Subjects: Pharmaceutical industry -- Quality control , Pharmaceutical technology , Drugs -- Standards
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10412 , http://hdl.handle.net/10948/566 , Pharmaceutical industry -- Quality control , Pharmaceutical technology , Drugs -- Standards
- Description: This investigation attempts to examine the influences of validation on pharmaceutical processes especially at a new manufacturing facility that has to meet international requirements, and fulfil a cost effective business strategy. At Aspen Pharmacare, a pharmaceutical organisation, there are two manufacturing facilities situated adjacent to each other, one new and one old. The new facility creates ideal opportunities to supply products to local and international markets. The investigation compares legal requirements from local and international regulatory authorities. Validation and qualification practices as well as the problems encountered during the different phases are discussed. Particular attention is given to the validation approach at the new Aspen facility. Problems and proposed solutions relating to the design review, installation, operational, and performance qualification are discussed. Validation of analytical methods for cleaning analysis, cleaning validation of equipment, and optimisation of some tablet manufacturing processes are described. Statistical evaluations of analytical results are included to find the optimum conditions for integrating new personnel with new processes and equipment. A business model reviews the cost of non-conformances of the enalapril maleate 10 mg tablets manufactured at the two manufacturing facilities. Finally the dissertation proves that validation is not only a regulatory requirement but that it also provides benefits such as adding value to the business, and ultimately reducing the cost of medicines.
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- Date Issued: 2007
Ionic liquids as media for electro-organic synthesis
- Authors: Kruger, Elna
- Date: 2007
- Subjects: Ionic solutions , Inorganic compounds -- Synthesis , Electrochemistry
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10413 , http://hdl.handle.net/10948/538 , Ionic solutions , Inorganic compounds -- Synthesis , Electrochemistry
- Description: The IL’s used in this study were either synthesized or commercially available. Preparation of the IL’s involved 2 step processes: firstly the heating of distilled 1-methylimidazole with distilled 1-chlorobutane under reflux to obtain 1-butyl-3- methylimidazolium chloride; secondly, the metathesis reaction of sodium tetrafluoroborate with 1-butyl-3-methylimidazolium chloride to obtain 1-butyl-3- methylimidazolium tetrafluoroborate. The addition of sodium tetrafluoroborate, sodium hexafluorophosphate and lithium trifluoromethane sulfonamide with 1-butyl-3-methylimidazolium chloride produced good yields of 1-butyl-3-methylimidazolium tetrafluoroborate, 1-butyl-3-methylimidazolium hexafluorophosphate and 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide respectively. The IL’s are hygroscopic and must be stored under a nitrogen atmosphere. The IL’s were analyzed using 1H and 13C NMR analysis with CDCl3 as solvent. The physical and chemical properties of these IL’s were compared to commercial products. The physical and chemical properties compared well to reference values from the literature. The physical properties measured include the density, conductivity and electrochemical window. The electrochemical window is dependant primarily on the resistance of the cation to reduction and the resistance of the anion to oxidation. The electrochemical windows of the IL’s were very similar to the reference windows obtained from literature and it can be seen that some IL’s have a slightly lower window which can be due to water present. Water content in the IL’s was determined with the use of a Karl Fischer titrator, with Hydranal 5 Composite as titrant and HPLC grade methanol as the base. The concentration of halide in the IL’s was determined with a Perkin-Elmer ICP-MS.
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- Date Issued: 2007
Isolation of a Clostridium Beijerinckii sLM01 cellulosome and the effect of sulphide on anaerobic digestion
- Authors: Mayende, Lungisa
- Date: 2007
- Subjects: Cellulose , Clostridium , Cellulase , Sulfides
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3973 , http://hdl.handle.net/10962/d1004032 , Cellulose , Clostridium , Cellulase , Sulfides
- Description: Cellulose is the most abundant and the most resistant and stable natural organic compound on earth. Enzyme hydrolysis is difficult because of its insolubility and heterogeneity. Some (anaerobic) microorganisms have overcome this by having a multienzyme system called the cellulosome. The aims of the study were to isolate a mesophilic Clostridium sp. from a biosulphidogenic bioreactor, to purify the cellulosome from this culture, to determine the cellulase and endoglucanase activities using Avicel and carboxymethylcellulose (CMC) as substrates and the dinitrosalicyclic (DNS) method. The organism was identified using 16S rDNA sequence analysis. The sequence obtained indicated that a strain of Clostridium beijerinckii was isolated. The cellulosome was purified from the putative C. beijerinckii sLM01 host culture using affinity chromatography purification and affinity digestion purification procedures. The cellulosomal and non-cellulosomal fractions of C. beijerinckii sLM01 were separated successfully, but the majority of the endoglucanase activity was lost during the Sepharose 4B chromatography step. These cellulosomal and non-cellulosomal fractions were characterised with regards to their pH and temperature optima and effector sensitivity. Increased additions of sulphide activated the cellulase activity of the cellulosomal and non-cellulosomal fractions up to 700 %, while increased additions of sulphate either increased the activity slightly or inhibited it dramatically, depending on the cellulosomal and non-cellulosomal fractions. Increased additions of cellobiose, glucose and acetate inhibited the cellulase and endoglucanase activities. pH optima of 5.0 and 7.5 were observed for cellulases and 5.0 for endoglucanases of the cellulosomal fraction. The noncellulosomal fraction exhibited a pH optimum of 7.5 for both cellulase and endoglucanase activities. Both fractions and enzymes exhibited a temperature optimum of 30 °C. The fundamental knowledge gained from the characterisation was applied to anaerobic digestion, where the effect of sulphide on the rate-limiting step was determined. Sulphide activated cellulase and endoglucanase activities and increased the % chemical oxygen demand (COD) removal rate. Levels of volatile fatty acids (VFAs) were higher in the bioreactor containing sulphide, substrate and C. beijerinckii. Sulphide therefore accelerated the rate-limiting step of anaerobic digestion.
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- Date Issued: 2007
Isolation of bioactive metabolites with activity against HIV-1 target proteins from extracts of Sutherlandia frutescens and Lobostemon trigonus
- Authors: Dambuza, Ntokozo Shirley
- Date: 2007
- Subjects: Medicinal plants -- Africa, Southern , HIV infections -- Alternative treatment -- Africa, Southern , Plant bioactive compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10335 , http://hdl.handle.net/10948/492 , http://hdl.handle.net/10948/d1012003 , Medicinal plants -- Africa, Southern , HIV infections -- Alternative treatment -- Africa, Southern , Plant bioactive compounds
- Description: Acquired Immunodeficiency Syndrome (AIDS) is a human disease caused by the human immunodeficiency virus type 1 (HIV-1) and it is one of the biggest social, economic and health challenges in the world. The Joint United Nations Programme on HIV/AIDS (UNAIDS) and World Health Organization (WHO) estimated that between 33.4 to 46.0 million people around the world were living with HIV/AIDS in December 2005 and the highest estimates are in the Sub-Saharan Africa (around 25 million). In more developed countries a combined antiretroviral therapy called highly active antiretroviral therapy (HAART) is used, which results in reduced progression to AIDS in most patients. Despite the beneficial effects of HAART, significant side effects are experienced by treated patients. In addition, most infected people live in countries where the treatment is very expensive or, in many cases, not available at all. These people therefore rely on medicinal plants for health care. In this study, aqueous extracts from Sutherlandia frutescens and Lobostemon trigonus were screened for potential anti-HIV activities in a series of in vitro enzymatic assays, including reverse transcriptase, HIV-1 protease and glycohydrolases. Two extracts of Sutherlandia leaves (SFL-1 and SFL-2) were prepared that inhibited HIV reverse transcriptase and a Lobostemon leaf extract (LTL) was shown to also inhibit this enzyme. All extracts were assayed at 1.25mg/ml. Tannin content was determined for all active extracts using a tannic acid assay. SFL-1 and SFL-2 were found to contain about 6 percent and 7 percent tannins, respectively, and LTL contained 31% tannins by weight. Tannins were removed using polyamide columns and three fractions were collected for each. The extracts were also fractionated with Sephadex G-25, Amberlite IR 120 and Dowex 1-X8 as size exclusion, cation exchange and anion exchange, respectively. Extracts were also fractionated by preparative thin layer chromatography where two compounds were separated from S. frutescens extract with high activity against reverse transcriptase while showing insignificant inhibition towards other enzymes tested. SFL-BFW-10 and SFL-WEF-7 inhibited reverse transcriptase by almost 100 percent and the IC50 values calculated for these compounds were 0.34 and 0.23mg/ml, respectively. Cytotoxicity of these compounds was evaluated on Chang liver cells and peripheral blood mononuclear cells (PBMCs). None of these compounds showed any significant inhibition of cell proliferation. The purity of these compounds could not be confirmed because there was insufficient material to use in the techniques required to show purity and identification. Therefore, TLC was used to determine the nature of these compounds. SFL-BFW-10 was identified as an organic acid and SFL-WEF-7 was identified as flavonoid.
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- Date Issued: 2007
Isolation, propagation and rapid molecular detection of the Kalahari truffle, a mycorrhizal fungus occurring in South Africa
- Authors: Adeleke, Rasheed Adegbola
- Date: 2007 , 2013-04-03
- Subjects: Truffles -- Kalahari Desert , Fungi -- Identification , Mycorrhizal fungi -- South Africa , Edible fungi -- South Africa , Mushroom culture -- South Africa , Fungi -- Cultures and culture media -- South Africa , Truffles -- South Africa , Truffles -- Lifecycles , Mycorrhizal fungi -- Lifecycles
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3889 , http://hdl.handle.net/10962/d1002951 , Truffles -- Kalahari Desert , Fungi -- Identification , Mycorrhizal fungi -- South Africa , Edible fungi -- South Africa , Mushroom culture -- South Africa , Fungi -- Cultures and culture media -- South Africa , Truffles -- South Africa , Truffles -- Lifecycles , Mycorrhizal fungi -- Lifecycles
- Description: Terfezia pfeilii is an edible mycorrhizal fungus that thrives in the Kalahari Desert of southern Africa. It is best known by desert dwellers for its flavour and as a source of nutrition. Although the genus Terfezia is generally regarded as being an ectomycorrhizal mycobiont, the exact mycorrhizal type formed by T. pfeilli and its' associated host plants remains uncertain. Discovery of the host plants for T. pfeilii would first be required in order to further investigate the life cycle and cultivation of this truffle. This study focussed on the isolation of mycelia from the ascocarp, optimising the growth conditions of the mycelial cultures, rapid molecular identification of T. pfeilii, investigation of potential helper bacteria and mycorrhizal synthesis experiments. T. pfeilii ascocarps were harvested from the Spitskop Nature Reserve in Upington, South Africa. Ascocarps were successfully identified using both morphological and molecular methods. Despite the delayed growth mostly caused by contaminating microorganisms, the isolation of T. pfeilii mycelia culture was successful. Molecular techniques were used to confirm the identity of the pure culture. Further studies were conducted on ways to improve the growth conditions of the mycelial culture on Fontana medium. An optimum temperature of 32°C, the addition of Bovine Serum Albumin as a nitrogen source and a pH of 7.5 significantly improved the growth of T. pfeilii in vitro. A rapid PeR-based molecular method was developed to speed up the identification of T. pfeilii. Specific primers that can exclusively amplify the ITS region of T. pfeilii were designed and used to identify both the ascocarps and the mycelial culture. The specificity of these primers was confirmed by their inability to amplify DNA from the isolates of contamining fungi obtained during the isolation process. Molecular comparison was made to confirm the reclassification of South African samples of T. pfeilii as Kalaharituber pfeilii as proposed by Ferdman et al.,(2005). However, in this study, the name T. pfeilii has been retained. A total of 17 bacterial isolates were obtained from the fruiting bodies of T. pfeaii and these were tested for stimulation of mycelial growth in vitro, indole production and phosphate solubilising capabilities. Bacterial isolates that showed potential to be Mycorrhization Helper Bacteria (MHB) were identified as Paenibacillus sp., Bacillus sp. and Rhizobium tropici. Selected plant seedlings were inoculated with T. pfeilii cultures or ascocarp slurry in order to re-establish the mycorrhizal association. After 8 months, light microscopy observations revealed an endomycorrhizal type association between Cynodon dactylon and T. pfeilii. This was confirmed with molecular analysis using specific T. pfeilii ITS primers. After 15 months, molecular methods confirmed Acacia erioloba as another host plant. These results have provided essential information paving the way for further investigation into the life cycle and biology of the Kalahari truffle. , KMBT_363 , Adobe Acrobat 9.53 Paper Capture Plug-in
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- Date Issued: 2007
Karen Horney : a psychobiographical study
- Authors: Green, Sarah-Jane
- Date: 2007
- Subjects: Horney, Karen, 1885-1952 Women psychologists Levinson, Daniel J., d.1994 Levinson, Daniel J., d.1994 -- The seasons of a woman's life
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3186 , http://hdl.handle.net/10962/d1008374
- Description: This psychobiography on the life of Karen Horney is an exploratory-descriptive study that aims to explore and describe Horney's life structure development according to Daniel Levinson's (1996) theory of adult development. The method of research uses a qualitative single-case research design that studies her life over her entire lifespan and highlights the individual characteristics of Horney in a holistic manner within her socio-historical context. A purposive sampling procedure was used to select Karen Horney as the subject of the research and only published data was analyzed in the research. Multiple sources of data are used to obtain the information in this research, including an autobiography and three biographies written by three different authors. This data is organized according to the temporal sequence of her lifespan and the developmental periods and time frame of Levinson's theory. Horney's life is described and explored to highlight those areas of her life that conform to the developmental theory and those aspects of her life that are not included in the theoretical constructs of Levinson 's theory. By exploring the significant relationships that Horney developed throughout her life with family, friends, work colleagues, community and religious affiliations, and the important issues in her development, this study verifies Levinson's theory by corroborating his conceptualisation of adult development as a process of sequential stages divided into four main eras of development, linked by three periods of transition. A shortcoming of Levinson's theory relates to his omission of self-esteem issues and religious concerns as relevant factors experienced during the era of pre-adulthood and spiritual concerns during the era of late adulthood.
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- Date Issued: 2007
Killing for the camera?: an investigation into the relationship between serial killers and the media
- Authors: Olivier, Erin Monique
- Date: 2007
- Subjects: Serial murderers -- Press coverage , Serial murders in mass media , Violence in mass media
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8411 , http://hdl.handle.net/10948/587 , http://hdl.handle.net/10948/d1011672 , Serial murderers -- Press coverage , Serial murders in mass media , Violence in mass media
- Description: This study focuses on the role of media portrayal and coverage in serial killing. The first objective of the study is to develop a conceptual structure that aids in the understanding of the cyclical relationship between media, serial killer, and audience. The media acts as a catalyst in this relationship, providing the stage on which serial killer and audience form a fatal relationship in which celebrity status forms the ultimate motivation. Media sensationalism of serial killing and the extreme glamorization in fictional representations has obviously negative consequences. In developing such a structure I hope to demonstrate that there is an alternative to the sensationalizing and glamorizing of serial killers in the media. This alternative will take the form education and a more documentary-style approach to films about serial murder. The study focuses mainly on developing a theoretical framework that emphasizes each of the three elements of the cyclical relationship mentioned above separately. The second chapter is devoted to the media and its role. The third chapter focuses on serial killers and the motivations involved. The fourth chapter deals with the audience attracted to serial killing as a source of identification. A number of thinkers’ work is used in coming to grips with this relationship, including both American and South African authors. The fifth and final chapter takes into consideration the moment of application by addressing the South African situation. I conclude by discussing the repercussions of media glamorization and possible documentary-style alternatives.
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- Date Issued: 2007
Laboratory investigation of a load carriage task observed in forestry
- Authors: Furney, Sheena Elizabeth
- Date: 2007
- Subjects: Work -- Physiological aspects , Foresters -- Workload , Human engineering , Lifting and carrying
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5145 , http://hdl.handle.net/10962/d1008188 , Work -- Physiological aspects , Foresters -- Workload , Human engineering , Lifting and carrying
- Description: The objective of the present study was to investigate and compare the human responses to two load carriage tasks performed with three different load masses and on three different gradients. The task of carrying hydrogel in one hand was observed in a silviculture industry and crude physiological and perceptual responses were measured. This task was simulated in a laboratory setting together with a suggested intervention of backpack carriage. Eighteen conditions were established which consisted of the two modes of carriage and a combination of three load masses (9kg, 12kg and 15kg) and three gradients (5%, 10% and 15%). Twenty eight Rhodes University female students comprised the sample and the experimental procedures were conducted on a Quinton treadmill. Each participant was required to complete nine of the eighteen conditions which were each four minutes in duration. Postural changes were assessed using lateral and posterior digital images taken at the second and fourth minute and compression and shearing forces were estimated with the ErgolmagerTM Physiological responses (heart rate, ventilation and metabolic responses) were measured continuously with the Quark b² and perceptual responses ('central' and 'local' RPE) were measured every minute during the experimentation and body discomfort was rated at the completion of each condition. Overall responses revealed that hand carriage (146 bt.min⁻¹ , 25.09 mIO₂. kg-l.min⁻¹) was generally found to be more physiologically stressful than backpack carriage (130 bt.min⁻¹, 22.15 mIO₂.kg⁻¹ .min⁻¹) independent of load mass and gradient. Physiological responses were higher (113 bt.min-1 to 174 bt.min⁻¹ ) in responses to increasing gradient as opposed to increasing load mass (104 bt.min-1 to 153 bt.min⁻¹ ) for both backpack and hand carriage. Categorisation using the guidelines of Sanders and McCormick (1993) allowed for classification of conditions, with respect to physiological responses, into 'moderate', 'heavy' and 'very heavy' stress. For almost all of the physiological responses the majority of conditions which were classified as 'moderate' were backpack carriage conditions and the conditions classified as 'very heavy' were mostly hand carriage conditions. In terms of postural responses hand carriage resulted in more strain and greater compression and shearing forces on the spine. In terms of the compression forces increasing gradient had a greater affect on backpack carriage (681 N to 935 N) compared to hand carriage (570N to 793N). In contrast, increasing load mass had a larger affect on hand carriage postures and compression forces (751 N to 935N) in comparison to backpack carriage (723N to 780N). Shearing forces were found to be worse in hand carriage conditions overall. Although participants generally underrated perceived exertion in relation to cardiorespiratory responses, these perceptions revealed that backpack carriage, with a mean 'central' RPE of 12 compared to 11 for hand carriage, was somewhat preferred to hand carriage and that increasing gradient was perceived to be marginally more straining than increasing load mass.
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- Date Issued: 2007
Land reform policies and human rights : a South African case study
- Authors: Fabbriciani, Antonio Antonino
- Date: 2007
- Subjects: Land reform -- South Africa -- Case studies , Human rights -- South Africa -- Case studies
- Language: English
- Type: Thesis , Masters , MPhil
- Identifier: vital:9042 , http://hdl.handle.net/10948/502 , Land reform -- South Africa -- Case studies , Human rights -- South Africa -- Case studies
- Description: This treatise begins with a discussion of different clauses of the Bill of Rights in the South African Constitution and the land reform policies of the South African government. The inequality and injustice caused by decades of apartheid land law forms the background of the land reform programme. The treatise addresses the consequences of this legacy on the implementation of the South African Constitution including the right to property. The discussion includes the three key elements of the land reform programme namely restitution, redistribution and tenure reform. The content of this treatise ranges over these three elements of land reform, applying constitutional issues to the relevant case law, The balancing and the reconciliation of rights and interest between the individual and the public in a just manner will be the barometer. The conclusion shows that the Constitution both protects existing rights and authorises the promotion of land reform within the framework of Section 25 of the Constitution, and that every aspect of the property clause has to be regarded as part of a constitional effort in balancing individual interest and public interest in terms of a constitutional order. It is my sincere hope that this treatise will contribute toward the achievement of equity, stability and by the values of an open and democratic society based on human dignity, freedom and human rights.
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- Date Issued: 2007
Limiting vulnerability exposure through effective patch management: threat mitigation through vulnerability remediation
- Authors: White, Dominic Stjohn Dolin
- Date: 2007 , 2007-02-08
- Subjects: Computer networks -- Security measures , Computer viruses , Computer security
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4629 , http://hdl.handle.net/10962/d1006510 , Computer networks -- Security measures , Computer viruses , Computer security
- Description: This document aims to provide a complete discussion on vulnerability and patch management. The first chapters look at the trends relating to vulnerabilities, exploits, attacks and patches. These trends describe the drivers of patch and vulnerability management and situate the discussion in the current security climate. The following chapters then aim to present both policy and technical solutions to the problem. The policies described lay out a comprehensive set of steps that can be followed by any organisation to implement their own patch management policy, including practical advice on integration with other policies, managing risk, identifying vulnerability, strategies for reducing downtime and generating metrics to measure progress. Having covered the steps that can be taken by users, a strategy describing how best a vendor should implement a related patch release policy is provided. An argument is made that current monthly patch release schedules are inadequate to allow users to most effectively and timeously mitigate vulnerabilities. The final chapters discuss the technical aspect of automating parts of the policies described. In particular the concept of 'defense in depth' is used to discuss additional strategies for 'buying time' during the patch process. The document then goes on to conclude that in the face of increasing malicious activity and more complex patching, solid frameworks such as those provided in this document are required to ensure an organisation can fully manage the patching process. However, more research is required to fully understand vulnerabilities and exploits. In particular more attention must be paid to threats, as little work as been done to fully understand threat-agent capabilities and activities from a day to day basis. , TeX output 2007.02.08:2212 , Adobe Acrobat 9.51 Paper Capture Plug-in
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- Date Issued: 2007
Local food choices and nutrition : a case study of amarewu in the FET consumer studies curriculum
- Authors: Kota, Lutho Siyabulela
- Date: 2007
- Subjects: Continuing education -- South Africa -- Eastern Cape -- Curricula Education -- South Africa -- Eastern Cape -- Curricula Ethnoscience -- South Africa -- Eastern Cape Indigenous peoples -- Study and teaching -- South Africa -- Eastern Cape Nutrition -- Study and teaching -- South Africa--Eastern Cape
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1666 , http://hdl.handle.net/10962/d1003549
- Description: This case study examines the introduction of Indigenous Knowledge (IK) in the Consumer Studies curriculum of Further Education and Training (FET). The research is centred on the use of enquiry methodologies involving learners observing parent demonstrations of the making of ‘amarewu’ and other activities centred on the propositional knowledge dealing with fermentation in the Consumer Studies curriculum. The research involved a review of curriculum documents, participant observation of a demonstration of local food practices related to ‘amarewu’ and learner research activities and interviews to review the developing learning interactions. The learning activities were focused on the learners’ researching the cultural and nutritional value of ‘amarewu’ and included an audit of food consumed in the community. What transpired from this study was that working with IK in the curriculum is possible. The inclusion of IK is not only possible but desirable and has exciting possibilities for relevance in contemporary education. The active involvement in parent demonstration engaged the learners in IK in their mother tongue, therefore indigenous knowledge has relevance. The curriculum concepts also enhanced the engagement by giving rise to more relevant knowledge and a respect for cultural matters. Intergenerational capital and subject concepts also enabled learners to engage with local nutritional problems and to come up with practical solutions. This study demonstrates how IK intergenerational capital in combination in combination with curriculum concepts (subject knowledge capital) can enhance relevance and the learners’ real engagement with local health and nutritional problems. Not only did the learners have culturally valued knowledge, but also knowledge that has a practical grasp of the problem and that they could use to engage relevant issues. These two views of knowledge join in learning and can be used to address health issues. I therefore recommend connection of cultural knowledge and conceptual knowledge to strengthen the revitalisation of cultural heritage, thus equalising it to the modern patterns of life and enhancing meaningful curriculum orientation.
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- Date Issued: 2007
Localisation of Theiler's Murine Encephalomyelitis virus non-structural proteins 2B, 2C, 2BC and 3A in BHK-21 cells, and the effect of amino acid substitutions in 2C on localisation and virus replication
- Authors: Murray, Lindsay
- Date: 2007
- Subjects: Encephalomyelitis -- Genetic aspects , Amino acid sequence , Picornaviruses , Viruses -- Reproduction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4090 , http://hdl.handle.net/10962/d1007722 , Encephalomyelitis -- Genetic aspects , Amino acid sequence , Picornaviruses , Viruses -- Reproduction
- Description: The picornavirus family includes significant human and animal viruses such as poliovirus (PV), human rhinovirus (HRV) and foot-and-mouth-disease virus (FMDV). Current disease treatment and control strategies are limited by an incomplete understanding of the interactions between the non-structural, replicative picornavirus proteins and host cell components. To investigate these interactions, Theiler's murine encephalomyelitis virus (TMEV) 2B, 2C, 2BC and 3A proteins were transiently expressed in BHK-21 cells and detected by indirect immunostaining and laser-scanning or epifluorescence microscopy. The signal of the 2B protein overlapped with that of the ER marker protein, ERp60, as well as that of the peripheral Golgi marker protein, β-COP. The 2C protein overlapped with ERp60 in a faint reticular stain, and localised to large punctate structures that partially overlapped with β-COP at higher levels of expression. The 2BC protein located to large perinuclear structures that overlapped exclusively with β-COP. The TMEV 3A protein signal overlapped with both ERp60 and β-COP stains, in addition in cells expressing the 3A protein the ER appeared swollen and bulbous while the Golgi was dispersed in some cells. 2C and 2BC proteins with C-terminal deletions localised in the same manner as the wild type proteins indicating that the localisation signals that determine subcellular localisation of the proteins are within the N-terminal 60 amino acids of the 2C protein. The significance of the high degree of conservation of the N-terminal domain of the 2C protein throughout the Picornaviridae was investigated through the introduction of amino acid substitution mutations at highly conserved residues in the N-terminal domain of 2C into the viral cDNA. Upon transfection of the viral RNA into BHK-21 cells, it was observed that substitution of amino acid residues 8, 18 and 29 abolished the ability of TMEV to induce cytopathic effect (CPE), while substitution of residues 4, 14 and 23 only attenuated the ability of TMEV to induce CPE. To determine whether amino acid substitution mutations would affect the localisation of the 2C protein, 2C proteins with substitution mutations at amino acids 4, 8, 14, 18, 23 and 29 were transiently expressed in BHK-21 cells and detected by indirect imrnunostaining and examination by laser-scanning confocal and epifluorescence microscopy. The 2C mutant 4, 8 and 29 proteins showed slightly altered localisation patterns compared to the wild type protein with a significant portion of the proteins localising in a perinuclear stain suggesting possible localisation to the nuclear envelop. The 2C mutant 14 and 18 proteins localised to a diffuse pattern in BHK-21 cells while the 2C mutant 23 protein located to small punctate structures that partially overlapped with the ERp60 stain but were completely separate from the β-COP stain. Finally, a hydrophilic, antigenic region of the 2C protein was expressed in frame with an N-terminal GST tag and was successfully purified on a pilot-scale and detected by Western analysis. This 2C178 peptide will be used to generate antibodies against the 2C and 2BC proteins for use in future studies. This study has furthered our knowledge of the localisation of the picornavirus 2B, 2C, 2BC and 3A proteins in host cells and identified a possible link between this localisation and an ability of TMEV to replicate in BHK-21 cells.
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- Date Issued: 2007
Making visual literacy meaningful in a rural context: an action research case study
- Authors: Mbelani, Madeyandile
- Date: 2007
- Subjects: Visual literacy -- Study and teaching (Secondary) -- South Africa -- Eastern Cape -- Case studies Education -- Research -- Case studies Education -- Research -- South Africa -- Eastern Cape Rural schools -- Research -- Case studies Rural schools -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1668 , http://hdl.handle.net/10962/d1003551
- Description: This thesis reports on a collaborative action research case study into the teaching of visual literacy to Grade 10 learners in a rural high school in the Eastern Cape, South Africa. Visual literacy is one of the critical aspects that have been incorporated in the teaching of English First Additional Language as required by the National Curriculum Statement (Grade 10-12), which has been implemented in Grade 10 as from 2006. With the aim of improving learners’ performance in visual literacy I designed a visual literacy unit that consisted of lesson plans running over 7 periods in 10 school days. In implementing the unit the learners were first grouped and then exposed to visual grammar and visual texts and then they critically viewed such texts and designed their own. Data was collected daily in the form of individual learner journals, researcher’s journal/diary, and copies were kept of activities done by learners (individually or in groups). Also, two teachers were invited as non-participant observers to each visit a lesson. Learner focus groups were conducted and critical friends were interviewed, tape recorded and transcribed. A camera was used to take still photographs to show learner activities in groups and during group presentations. The data revealed that visual literacy could be taught meaningfully in a rural high school as the learners could identify, cut, paste and discuss elements of visual language and they finally designed their own advertisements in groups. In the analysis of data the following factors emerged as hindrances for successful teaching of visual literacy in a rural high school: lack of resources; learners’ lack of a foundation in visual literacy from Grades 7-9; and problems revolving around time management and pacing. As action research comes in spirals, this research represented the first one and the researcher found the study an eye opener and a foundation to build on in the second spiral (that is not part of this research).
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- Date Issued: 2007
Marital turnarounds : an exploratory qualitative study
- Authors: Ntlokwana, Nomalungelo
- Date: 2007
- Subjects: Remarriage -- South Africa -- Nelson Mandela Metropole
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9933 , http://hdl.handle.net/10948/585 , Remarriage -- South Africa -- Nelson Mandela Metropole
- Description: The purpose of this study was to study marital turnarounds by focusing on the exploration of the reasons for couples remarrying each other. A comprehensive approach based on human ecology provided a framework for exploring the reasons for marital turnarounds ranging from micro to macro level factors. Data was collected through six semi-structured interviews, with two African marital turnaround couples from the Nelson Mandela Metropole. Data analysis was carried out systematically through a stepwise process. Data verification and trustworthiness was also performed. Five major themes emerged from the data analysis of the interviews as follows: Culture and religious influences, individual influences, personal growth, family influences and the influence of friends. Culture and religion emerged as powerful theme in shaping decisions about marital turnaround. An important sub-theme was that, in a culture that continues to value marriage as a permanent commitment, spouses internalised the social and religious norm of marriage as a permanent commitment.
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- Date Issued: 2007