Polymers, catalysts and nanostructures a hybrid approach to biomolecule detection
- Authors: Frith, Kelly-Anne
- Date: 2009
- Subjects: Polymers , Nanostructured materials , Biomolecules , Tryptophan , Melatonin , Electrodes , Electrochemistry , Tryptophan oxygenase
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3980 , http://hdl.handle.net/10962/d1004039 , Polymers , Nanostructured materials , Biomolecules , Tryptophan , Melatonin , Electrodes , Electrochemistry , Tryptophan oxygenase
- Description: The main goals in electroanalytical sensing are towards improved sensitivity and selectivity, or specificity, of an analyte. There are several approaches to achieving these goals with the main approach being modification of an electrode surface with synthetic or natural catalysts (enzymes), polymers and also utilisation of nanostructured materials. At present, there is a strong movement towards hybrid sensing which couple different properties of two or more surface modification approaches. In this thesis, a range of these surface modifications were explored for analysis and detection of two main analytes: the amino acid, tryptophan (Trp); and, the neurotransmitter, dopamine (DA). Specifically, this thesis aimed to utilise these methods to enhance the sensitivity and selectivity for Trp over an interferent, the indoleamine, melatonin (Mel); and, DA over the vitamin, ascorbic acid (AA). For Trp detection, immobilisation of an enzyme, Tryptophanase (Trpase) resulted in poor selectivity for the analyte. However, enhanced sensitivity and selectivity was achieved through pH manipulation of the electrolyte medium at a Nafion®-modified electrode surface for both Trp and Mel. At pH 3.0, the Mel and Trp anodic peak potentials were sufficiently resolved allowing for an LOD of 1.60 and 1.62 nM,respectively, and permitting the accurate analysis of Trp in a dietary supplement containing Mel. Multi-walled carbon nanotubes (MWCNTs) suspended in Nafion® exhibited further increases in the signal responses of these analytes at pH 3.0 and 7.4 with minimal change in the resolution of the anodic peaks. A lower sensitivity was, therefore, observed at the Nafion® and MWCNT modified electrode compared to the Nafion®-modified electrode at pH 3.0 with LODs of 0.59 and 0.80 nM exhibited for Trp and Mel, respectively. Enhanced selectivity for Trp in the presence of Mel can be achieved with MWCNTs in the presence of metallotetrasulphonated phthalocyanines (MTSPcs) particularly at pH 3.0, owing to cation exchange effects. However, the lack of sensitivity towards Trp, and even Mel, at this CoTSPc and MWCNT modified electrode remains a drawback. For DA, detection at the MWCNT and Nafion® surface resulted in improved sensitivity over that of both the bare electrode (613.0 nM) and the Nafion® modified electrode (1045.1 nM) with a calculated LOD of 133.9 nM at this layer. Furthermore, improvements in the selectivity of DA were achieved at the Nafion® and MWCNT modified electrode as exclusion of AA (150 μM) was achieved. At the MWCNT and CoTSPc surface, AA was excluded up to 130 μM with sensitivity for DA extending as low as 14.3 nM, far greater than observed for Trp and Mel. These concentrations are well within physiological concentration ranges and represent the most significant solution yet in terms of AA exclusion and enhanced sensitivity for DA. An examination of the surface layering by impedance spectroscopy and atomic force microscopy indicates that the success of the hybrid sensor utilising CoTSPc and MWCNTs lay in improved dispersion of MWCNTs and improved electron transfer kinetics, facilitated by the net charge of the materials present. This thesis, thus, showed the utility of a judicious selection of synthetic and biological catalysts, polymers and carbon nanomaterials towards a hybrid approach to the electrochemical sensing of Trp, Mel, DA and AA with focus on sensitivity and selectivity of these analytes.
- Full Text:
- Authors: Frith, Kelly-Anne
- Date: 2009
- Subjects: Polymers , Nanostructured materials , Biomolecules , Tryptophan , Melatonin , Electrodes , Electrochemistry , Tryptophan oxygenase
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3980 , http://hdl.handle.net/10962/d1004039 , Polymers , Nanostructured materials , Biomolecules , Tryptophan , Melatonin , Electrodes , Electrochemistry , Tryptophan oxygenase
- Description: The main goals in electroanalytical sensing are towards improved sensitivity and selectivity, or specificity, of an analyte. There are several approaches to achieving these goals with the main approach being modification of an electrode surface with synthetic or natural catalysts (enzymes), polymers and also utilisation of nanostructured materials. At present, there is a strong movement towards hybrid sensing which couple different properties of two or more surface modification approaches. In this thesis, a range of these surface modifications were explored for analysis and detection of two main analytes: the amino acid, tryptophan (Trp); and, the neurotransmitter, dopamine (DA). Specifically, this thesis aimed to utilise these methods to enhance the sensitivity and selectivity for Trp over an interferent, the indoleamine, melatonin (Mel); and, DA over the vitamin, ascorbic acid (AA). For Trp detection, immobilisation of an enzyme, Tryptophanase (Trpase) resulted in poor selectivity for the analyte. However, enhanced sensitivity and selectivity was achieved through pH manipulation of the electrolyte medium at a Nafion®-modified electrode surface for both Trp and Mel. At pH 3.0, the Mel and Trp anodic peak potentials were sufficiently resolved allowing for an LOD of 1.60 and 1.62 nM,respectively, and permitting the accurate analysis of Trp in a dietary supplement containing Mel. Multi-walled carbon nanotubes (MWCNTs) suspended in Nafion® exhibited further increases in the signal responses of these analytes at pH 3.0 and 7.4 with minimal change in the resolution of the anodic peaks. A lower sensitivity was, therefore, observed at the Nafion® and MWCNT modified electrode compared to the Nafion®-modified electrode at pH 3.0 with LODs of 0.59 and 0.80 nM exhibited for Trp and Mel, respectively. Enhanced selectivity for Trp in the presence of Mel can be achieved with MWCNTs in the presence of metallotetrasulphonated phthalocyanines (MTSPcs) particularly at pH 3.0, owing to cation exchange effects. However, the lack of sensitivity towards Trp, and even Mel, at this CoTSPc and MWCNT modified electrode remains a drawback. For DA, detection at the MWCNT and Nafion® surface resulted in improved sensitivity over that of both the bare electrode (613.0 nM) and the Nafion® modified electrode (1045.1 nM) with a calculated LOD of 133.9 nM at this layer. Furthermore, improvements in the selectivity of DA were achieved at the Nafion® and MWCNT modified electrode as exclusion of AA (150 μM) was achieved. At the MWCNT and CoTSPc surface, AA was excluded up to 130 μM with sensitivity for DA extending as low as 14.3 nM, far greater than observed for Trp and Mel. These concentrations are well within physiological concentration ranges and represent the most significant solution yet in terms of AA exclusion and enhanced sensitivity for DA. An examination of the surface layering by impedance spectroscopy and atomic force microscopy indicates that the success of the hybrid sensor utilising CoTSPc and MWCNTs lay in improved dispersion of MWCNTs and improved electron transfer kinetics, facilitated by the net charge of the materials present. This thesis, thus, showed the utility of a judicious selection of synthetic and biological catalysts, polymers and carbon nanomaterials towards a hybrid approach to the electrochemical sensing of Trp, Mel, DA and AA with focus on sensitivity and selectivity of these analytes.
- Full Text:
Predictability of Geomagnetically Induced Currents using neural networks
- Authors: Lotz, Stefan
- Date: 2009
- Subjects: Advanced Composition Explorer (Artificial satellite) , Geomagnetism , Electromagnetic induction , Neural networks (Computer science) , Artificial intelligence
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5483 , http://hdl.handle.net/10962/d1005269 , Advanced Composition Explorer (Artificial satellite) , Geomagnetism , Electromagnetic induction , Neural networks (Computer science) , Artificial intelligence
- Description: It is a well documented fact that Geomagnetically Induced Currents (GIC’s) poses a significant threat to ground-based electric conductor networks like oil pipelines, railways and powerline networks. A study is undertaken to determine the feasibility of using artificial neural network models to predict GIC occurrence in the Southern African power grid. The magnitude of an induced current at a specific location on the Earth’s surface is directly related to the temporal derivative of the geomagnetic field (specifically its horizontal components) at that point. Hence, the focus of the problem is on the prediction of the temporal variations in the horizontal geomagnetic field (@Bx/@t and @By/@t). Artificial neural networks are used to predict @Bx/@t and @By/@t measured at Hermanus, South Africa (34.27◦ S, 19.12◦ E) with a 30 minute prediction lead time. As input parameters to the neural networks, insitu solar wind measurements made by the Advanced Composition Explorer (ACE) satellite are used. The results presented here compare well with similar models developed at high-latitude locations (e.g. Sweden, Finland, Canada) where extensive GIC research has been undertaken. It is concluded that it would indeed be feasible to use a neural network model to predict GIC occurrence in the Southern African power grid, provided that GIC measurements, powerline configuration and network parameters are made available.
- Full Text:
- Authors: Lotz, Stefan
- Date: 2009
- Subjects: Advanced Composition Explorer (Artificial satellite) , Geomagnetism , Electromagnetic induction , Neural networks (Computer science) , Artificial intelligence
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5483 , http://hdl.handle.net/10962/d1005269 , Advanced Composition Explorer (Artificial satellite) , Geomagnetism , Electromagnetic induction , Neural networks (Computer science) , Artificial intelligence
- Description: It is a well documented fact that Geomagnetically Induced Currents (GIC’s) poses a significant threat to ground-based electric conductor networks like oil pipelines, railways and powerline networks. A study is undertaken to determine the feasibility of using artificial neural network models to predict GIC occurrence in the Southern African power grid. The magnitude of an induced current at a specific location on the Earth’s surface is directly related to the temporal derivative of the geomagnetic field (specifically its horizontal components) at that point. Hence, the focus of the problem is on the prediction of the temporal variations in the horizontal geomagnetic field (@Bx/@t and @By/@t). Artificial neural networks are used to predict @Bx/@t and @By/@t measured at Hermanus, South Africa (34.27◦ S, 19.12◦ E) with a 30 minute prediction lead time. As input parameters to the neural networks, insitu solar wind measurements made by the Advanced Composition Explorer (ACE) satellite are used. The results presented here compare well with similar models developed at high-latitude locations (e.g. Sweden, Finland, Canada) where extensive GIC research has been undertaken. It is concluded that it would indeed be feasible to use a neural network model to predict GIC occurrence in the Southern African power grid, provided that GIC measurements, powerline configuration and network parameters are made available.
- Full Text:
Preservation or exploitation? : a study of the development of the mining rights legislation on the Witwatersrand goldfields from 1886 to 2008
- Authors: Stott, Joan
- Date: 2009
- Subjects: Ostrom, Elinor Chamber of Mines of South Africa Gold mines and mining -- South Africa -- Witwatersrand -- History Mineral industries -- Finance -- South Africa Mining law -- South Africa
- Language: English
- Type: Thesis , Masters , MEcon
- Identifier: vital:988 , http://hdl.handle.net/10962/d1002723
- Description: Elinor Ostrom (2005: 238) assumes that in understanding the make up and behaviour of institutional systems governing natural resources: “Resource users are explicitly thought of as rational egoists who plunder local resources so as to maximise their own short-term benefits. Government officials are implicitly depicted, on the other hand, as seeking, the more general public interest, having the relevant information at hand and the capability of designing optimal policies.” This thesis examines the validity of this assumption through an historical analysis of the deep-level gold mining industry of the Witwatersrand, South Africa. The main focus of the assessment is on the institutions of ownership – that is, the development of mining rights and title legislation between 1886 and 2008. The study looks at the legislations’ transformation and implementation from the perspective of the gold mining industry – made up of the mining finance houses and the Chamber of Mines of South Africa – and that of the state. The transformation of the mining industry’s institutional framework was both a choice by government as well as that of the firms in the mining industry. The theoretical framework is constructed from four areas of economic thought. These include: the neoclassical and Keynesian schools of macroeconomic thought; industrial organisation and its relevance to the relationship between firms and the market; institutional and new institutional economics; and finally property rights. The determinants of policy design and the impact of such design on firms and industry is examined. The development, implementation and use of the aforementioned legislation is examined from two perspectives, namely, that of preserver or exploiter. Throughout the history of this prominent South African industry, the motivation for action from the industry or government has oscillated between the two extremes of preserver or exploiter over the time period examined. The conclusion is drawn on an overall and broad focus of actions – with a strong focus on the most recent developments in mining legislation – post-1992.
- Full Text:
- Authors: Stott, Joan
- Date: 2009
- Subjects: Ostrom, Elinor Chamber of Mines of South Africa Gold mines and mining -- South Africa -- Witwatersrand -- History Mineral industries -- Finance -- South Africa Mining law -- South Africa
- Language: English
- Type: Thesis , Masters , MEcon
- Identifier: vital:988 , http://hdl.handle.net/10962/d1002723
- Description: Elinor Ostrom (2005: 238) assumes that in understanding the make up and behaviour of institutional systems governing natural resources: “Resource users are explicitly thought of as rational egoists who plunder local resources so as to maximise their own short-term benefits. Government officials are implicitly depicted, on the other hand, as seeking, the more general public interest, having the relevant information at hand and the capability of designing optimal policies.” This thesis examines the validity of this assumption through an historical analysis of the deep-level gold mining industry of the Witwatersrand, South Africa. The main focus of the assessment is on the institutions of ownership – that is, the development of mining rights and title legislation between 1886 and 2008. The study looks at the legislations’ transformation and implementation from the perspective of the gold mining industry – made up of the mining finance houses and the Chamber of Mines of South Africa – and that of the state. The transformation of the mining industry’s institutional framework was both a choice by government as well as that of the firms in the mining industry. The theoretical framework is constructed from four areas of economic thought. These include: the neoclassical and Keynesian schools of macroeconomic thought; industrial organisation and its relevance to the relationship between firms and the market; institutional and new institutional economics; and finally property rights. The determinants of policy design and the impact of such design on firms and industry is examined. The development, implementation and use of the aforementioned legislation is examined from two perspectives, namely, that of preserver or exploiter. Throughout the history of this prominent South African industry, the motivation for action from the industry or government has oscillated between the two extremes of preserver or exploiter over the time period examined. The conclusion is drawn on an overall and broad focus of actions – with a strong focus on the most recent developments in mining legislation – post-1992.
- Full Text:
Protein secondary structure prediction using neural networks and support vector machines
- Authors: Tsilo, Lipontseng Cecilia
- Date: 2009
- Subjects: Neural networks (Computer science) , Support vector machines , Proteins -- Structure -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5569 , http://hdl.handle.net/10962/d1002809 , Neural networks (Computer science) , Support vector machines , Proteins -- Structure -- Mathematical models
- Description: Predicting the secondary structure of proteins is important in biochemistry because the 3D structure can be determined from the local folds that are found in secondary structures. Moreover, knowing the tertiary structure of proteins can assist in determining their functions. The objective of this thesis is to compare the performance of Neural Networks (NN) and Support Vector Machines (SVM) in predicting the secondary structure of 62 globular proteins from their primary sequence. For each NN and SVM, we created six binary classifiers to distinguish between the classes’ helices (H) strand (E), and coil (C). For NN we use Resilient Backpropagation training with and without early stopping. We use NN with either no hidden layer or with one hidden layer with 1,2,...,40 hidden neurons. For SVM we use a Gaussian kernel with parameter fixed at = 0.1 and varying cost parameters C in the range [0.1,5]. 10- fold cross-validation is used to obtain overall estimates for the probability of making a correct prediction. Our experiments indicate for NN and SVM that the different binary classifiers have varying accuracies: from 69% correct predictions for coils vs. non-coil up to 80% correct predictions for stand vs. non-strand. It is further demonstrated that NN with no hidden layer or not more than 2 hidden neurons in the hidden layer are sufficient for better predictions. For SVM we show that the estimated accuracies do not depend on the value of the cost parameter. As a major result, we will demonstrate that the accuracy estimates of NN and SVM binary classifiers cannot distinguish. This contradicts a modern belief in bioinformatics that SVM outperforms other predictors.
- Full Text:
- Authors: Tsilo, Lipontseng Cecilia
- Date: 2009
- Subjects: Neural networks (Computer science) , Support vector machines , Proteins -- Structure -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5569 , http://hdl.handle.net/10962/d1002809 , Neural networks (Computer science) , Support vector machines , Proteins -- Structure -- Mathematical models
- Description: Predicting the secondary structure of proteins is important in biochemistry because the 3D structure can be determined from the local folds that are found in secondary structures. Moreover, knowing the tertiary structure of proteins can assist in determining their functions. The objective of this thesis is to compare the performance of Neural Networks (NN) and Support Vector Machines (SVM) in predicting the secondary structure of 62 globular proteins from their primary sequence. For each NN and SVM, we created six binary classifiers to distinguish between the classes’ helices (H) strand (E), and coil (C). For NN we use Resilient Backpropagation training with and without early stopping. We use NN with either no hidden layer or with one hidden layer with 1,2,...,40 hidden neurons. For SVM we use a Gaussian kernel with parameter fixed at = 0.1 and varying cost parameters C in the range [0.1,5]. 10- fold cross-validation is used to obtain overall estimates for the probability of making a correct prediction. Our experiments indicate for NN and SVM that the different binary classifiers have varying accuracies: from 69% correct predictions for coils vs. non-coil up to 80% correct predictions for stand vs. non-strand. It is further demonstrated that NN with no hidden layer or not more than 2 hidden neurons in the hidden layer are sufficient for better predictions. For SVM we show that the estimated accuracies do not depend on the value of the cost parameter. As a major result, we will demonstrate that the accuracy estimates of NN and SVM binary classifiers cannot distinguish. This contradicts a modern belief in bioinformatics that SVM outperforms other predictors.
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Public discourses on choice of termination of pregnancy in a rural area of the Eastern Cape Province in South Africa
- Authors: Sigcau, Nomakhosi
- Date: 2009
- Subjects: Abortion -- South Africa -- Eastern Cape Abortion -- Public opinion Abortion -- Moral and ethical aspects Abortion -- Social aspects Abortion -- Psychological aspects Discourse analysis -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3055 , http://hdl.handle.net/10962/d1002564
- Description: A period of ten years has elapsed since the Choice on Termination of Pregnancy Act came into effect. Little has been done in South Africa to investigate public discourses concerning termination of pregnancy since the implementation of the Act. The social context and the quality of available support systems determine the outcome of the women’s feelings after the abortion. Knowledge about the social context is important, as it will help to understand the complexities and nuances of abortion. The aim of the research is to explore public discourses on Choice on Termination of Pregnancy (CTOP), and the potential implications of these discourses on the use of the CTOP service. The sample consisted of 23 black isiXhosa-speaking participants from the rural area of the Eastern Cape Province in South Africa. Four focus groups coming from different age groups (between the ages 18 and 52) with both men and women participated in the study. Fictitious vignettes that tap into two different scenarios regarding abortion based on women’s stories were used. Discourses that emerged from people’s text are explained, described and interpreted through a discourse analysis. Since the study was interested in public discourses it led to the discovery of 17 interpretative repertoires as follows: social stigma, abortion equated to murder, degradation of society, pregnancy as an irresponsible act, conditional acceptance, TOP in the context of marriage, future potentiality invested in the foetus, dehumanizing foetus into a clot, shared decision making responsibility, gender dynamics interpretative repertoire, negative post abortion consequences, the scolding versus the supportive nurse interpretative repertoire, alternatives interpretative repertoire, rights versus no responsibility interpretative repertoire, more knowledge needed, male and female or generational differentiation repertoire, and the positive effects repertoire. Abortion is opposed on religious and cultural grounds. TOP has been legalized in South Africa but with this a debate and conflicting views have arisen. These variations in people’s discourses may limit access to TOP for women who need the service.
- Full Text:
- Authors: Sigcau, Nomakhosi
- Date: 2009
- Subjects: Abortion -- South Africa -- Eastern Cape Abortion -- Public opinion Abortion -- Moral and ethical aspects Abortion -- Social aspects Abortion -- Psychological aspects Discourse analysis -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3055 , http://hdl.handle.net/10962/d1002564
- Description: A period of ten years has elapsed since the Choice on Termination of Pregnancy Act came into effect. Little has been done in South Africa to investigate public discourses concerning termination of pregnancy since the implementation of the Act. The social context and the quality of available support systems determine the outcome of the women’s feelings after the abortion. Knowledge about the social context is important, as it will help to understand the complexities and nuances of abortion. The aim of the research is to explore public discourses on Choice on Termination of Pregnancy (CTOP), and the potential implications of these discourses on the use of the CTOP service. The sample consisted of 23 black isiXhosa-speaking participants from the rural area of the Eastern Cape Province in South Africa. Four focus groups coming from different age groups (between the ages 18 and 52) with both men and women participated in the study. Fictitious vignettes that tap into two different scenarios regarding abortion based on women’s stories were used. Discourses that emerged from people’s text are explained, described and interpreted through a discourse analysis. Since the study was interested in public discourses it led to the discovery of 17 interpretative repertoires as follows: social stigma, abortion equated to murder, degradation of society, pregnancy as an irresponsible act, conditional acceptance, TOP in the context of marriage, future potentiality invested in the foetus, dehumanizing foetus into a clot, shared decision making responsibility, gender dynamics interpretative repertoire, negative post abortion consequences, the scolding versus the supportive nurse interpretative repertoire, alternatives interpretative repertoire, rights versus no responsibility interpretative repertoire, more knowledge needed, male and female or generational differentiation repertoire, and the positive effects repertoire. Abortion is opposed on religious and cultural grounds. TOP has been legalized in South Africa but with this a debate and conflicting views have arisen. These variations in people’s discourses may limit access to TOP for women who need the service.
- Full Text:
Purification and characterization of fructosyltransferase for the synthesis of short-chain fructo-oligosaccharides and investigation into thier anti-carcinogenic properties
- Authors: Nemukula, Aluwani
- Date: 2009
- Subjects: Oligosaccharides , Polygalacturonase , Aspergillus , Fructose , Inulin , Cancer -- Prevention , Cancer -- Research , Carcinogens , High performance liquid chromatography
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3927 , http://hdl.handle.net/10962/d1003986 , Oligosaccharides , Polygalacturonase , Aspergillus , Fructose , Inulin , Cancer -- Prevention , Cancer -- Research , Carcinogens , High performance liquid chromatography
- Description: There is a growing attention in the synthesis of fructo-oligosaccharides (FOS) due to their excellent bio-functional and health-promoting properties. The current production processes are limited to chemical hydrolysis reactions of plant extracts, which are often associated with several drawbacks. In this study, fructosyltransferase (FTase) and polygalacturonase (PGase) activities, present in a commercial enzyme preparation (Pectinex® Ultra SP-L) sourced from Aspergillus aculeatus, have been separated and fully purified by anion-exchange and sizeexclusion chromatography. The FTase possesses fructosyl transfer activity for FOS synthesis and the PGase has pectin hydrolytic activity. Fructosyltransferase is a single-band protein with a molecular weight of 85 kDa, whereas PGase is a distinct protein of 40 kDa. The temperature and pH optima of FTase were 60 ºC and 6.0, with a half-life of 8 h; while that for PGase were 40 ºC and 6.0, respectively. FTase was slightly inhibited in the presence of Ni²⁺, Mg²⁺ and urea; but PGase was more susceptible to divalent ions such as Ca²⁺, Mg²⁺ and Mn²⁺. The kinetic parameters (Km and Vmax) of FTase for the hydrolysis of β-(2→1) linkages from sucrose were 752.3 mM and 120.5 μmol.min⁻¹.mL⁻¹, respectively; whereas the same parameters for pectin hydrolysis by PGase were 13.0 mg.mL⁻¹ and 263 μmol.min-1.mL⁻¹, respectively. The purified FTase was able to transfer fructosyl residues from sucrose, synthesizing the corresponding chains of FOS. PGase was relatively stable at 40 ºC (t½ > 3 h), depolymerizing the pectin backbone while releasing the inulins from within the chicory roots. Analysis of various mixtures of FOS by mass spectrometry, HPLC and ¹H-NMR was undertaken. Results indicated that MS with electrospray ionization and ¹H-NMR are capable of providing relative quantitative data of the FOS present in the mixtures. The pharmaceutical effects of various sc-FOS (0.5%, v/v) and SCFA (0.3%, v/v) on certain bacterial enzymes (β-glucuronidase, urease and β-glucosidase) associated with the formation of carcinogens were also studied. These enzyme activities were not directly influenced by the sc-FOS, but were found to be remarkably decreased by SCFA, pointing toward the prebiotic effect of FOS in intestinal microflora modulation.
- Full Text:
- Authors: Nemukula, Aluwani
- Date: 2009
- Subjects: Oligosaccharides , Polygalacturonase , Aspergillus , Fructose , Inulin , Cancer -- Prevention , Cancer -- Research , Carcinogens , High performance liquid chromatography
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3927 , http://hdl.handle.net/10962/d1003986 , Oligosaccharides , Polygalacturonase , Aspergillus , Fructose , Inulin , Cancer -- Prevention , Cancer -- Research , Carcinogens , High performance liquid chromatography
- Description: There is a growing attention in the synthesis of fructo-oligosaccharides (FOS) due to their excellent bio-functional and health-promoting properties. The current production processes are limited to chemical hydrolysis reactions of plant extracts, which are often associated with several drawbacks. In this study, fructosyltransferase (FTase) and polygalacturonase (PGase) activities, present in a commercial enzyme preparation (Pectinex® Ultra SP-L) sourced from Aspergillus aculeatus, have been separated and fully purified by anion-exchange and sizeexclusion chromatography. The FTase possesses fructosyl transfer activity for FOS synthesis and the PGase has pectin hydrolytic activity. Fructosyltransferase is a single-band protein with a molecular weight of 85 kDa, whereas PGase is a distinct protein of 40 kDa. The temperature and pH optima of FTase were 60 ºC and 6.0, with a half-life of 8 h; while that for PGase were 40 ºC and 6.0, respectively. FTase was slightly inhibited in the presence of Ni²⁺, Mg²⁺ and urea; but PGase was more susceptible to divalent ions such as Ca²⁺, Mg²⁺ and Mn²⁺. The kinetic parameters (Km and Vmax) of FTase for the hydrolysis of β-(2→1) linkages from sucrose were 752.3 mM and 120.5 μmol.min⁻¹.mL⁻¹, respectively; whereas the same parameters for pectin hydrolysis by PGase were 13.0 mg.mL⁻¹ and 263 μmol.min-1.mL⁻¹, respectively. The purified FTase was able to transfer fructosyl residues from sucrose, synthesizing the corresponding chains of FOS. PGase was relatively stable at 40 ºC (t½ > 3 h), depolymerizing the pectin backbone while releasing the inulins from within the chicory roots. Analysis of various mixtures of FOS by mass spectrometry, HPLC and ¹H-NMR was undertaken. Results indicated that MS with electrospray ionization and ¹H-NMR are capable of providing relative quantitative data of the FOS present in the mixtures. The pharmaceutical effects of various sc-FOS (0.5%, v/v) and SCFA (0.3%, v/v) on certain bacterial enzymes (β-glucuronidase, urease and β-glucosidase) associated with the formation of carcinogens were also studied. These enzyme activities were not directly influenced by the sc-FOS, but were found to be remarkably decreased by SCFA, pointing toward the prebiotic effect of FOS in intestinal microflora modulation.
- Full Text:
Recontextualising issues in the 'NISTCOL" environmental education curriculum module for Primary Diploma by Distance Learning in Zambia
- Authors: Moose, John
- Date: 2009
- Subjects: Environmental education -- Zambia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1722 , http://hdl.handle.net/10962/d1003605
- Description: This study investigated the recontextualising of the NISTCOL environmental education curriculum module for the Primary Teachers’ Diploma by Distance Learning (PTDDL) in Zambia. It focused on three case sites, the NISTCOL EE curriculum module for PTDDL and the University of Zambia, the National In-Service Teacher’s College (NISTCOL) and four basic schools: chalimbana, Bimbe, Chongwe and Silver Rest. The study looked at teacher professional development in environmental education in Zambia. The aims and goals were to investigate the NISTCOL EE curriculum and identifying issues that were associated with lecturers in the recontextualising process and further examine what issues were associated with curriculum recontextualisation in Zambian schools. For the conceptual constructs, the research drew on Bernstein’s (1990) framework of pedagogic discourse, it traced how the pedagogic discourse was de-located from the field of production and re-located into the pedagogic practice of each stated case above. It further examined the continuities, discontinuities and changes in the official environmental discourse as it was recontextualised. The study particularly focused on Bernstein’s conceptual constructs of selective appropriation and ideological transformation. In each case the Bernstein framework is used to analyze the process to identify recontextualising issues influencing pedagogical practice in the Zambian schools and college. The study revealed that each case under focus was unique but through examining the environmental discourse and pedagogical discourse in each case recontextalising issues were identified. Each case was influenced by different factors, such as lack of policy synergy, lack of EE vision at national level in Zambia, experience and knowledge, ideologies and emphasis, and depth of engagement. These factors provided me with a deeper insight into the curriculum recontextualising processes in Zambian schools.
- Full Text:
- Authors: Moose, John
- Date: 2009
- Subjects: Environmental education -- Zambia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1722 , http://hdl.handle.net/10962/d1003605
- Description: This study investigated the recontextualising of the NISTCOL environmental education curriculum module for the Primary Teachers’ Diploma by Distance Learning (PTDDL) in Zambia. It focused on three case sites, the NISTCOL EE curriculum module for PTDDL and the University of Zambia, the National In-Service Teacher’s College (NISTCOL) and four basic schools: chalimbana, Bimbe, Chongwe and Silver Rest. The study looked at teacher professional development in environmental education in Zambia. The aims and goals were to investigate the NISTCOL EE curriculum and identifying issues that were associated with lecturers in the recontextualising process and further examine what issues were associated with curriculum recontextualisation in Zambian schools. For the conceptual constructs, the research drew on Bernstein’s (1990) framework of pedagogic discourse, it traced how the pedagogic discourse was de-located from the field of production and re-located into the pedagogic practice of each stated case above. It further examined the continuities, discontinuities and changes in the official environmental discourse as it was recontextualised. The study particularly focused on Bernstein’s conceptual constructs of selective appropriation and ideological transformation. In each case the Bernstein framework is used to analyze the process to identify recontextualising issues influencing pedagogical practice in the Zambian schools and college. The study revealed that each case under focus was unique but through examining the environmental discourse and pedagogical discourse in each case recontextalising issues were identified. Each case was influenced by different factors, such as lack of policy synergy, lack of EE vision at national level in Zambia, experience and knowledge, ideologies and emphasis, and depth of engagement. These factors provided me with a deeper insight into the curriculum recontextualising processes in Zambian schools.
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Redress : debates informing exhibitions and acquisitions in selected South African public art galleries (1990-1994)
- Authors: Cook, Shashi Chailey
- Date: 2009
- Subjects: South African National Gallery Exhibitions Durban Art Gallery Exhibitions Joahnnesburg Art Gallery Exhibitions Art -- South Africa -- Exhibitions Art, South African Exhibitions South Africa History Exhibitions
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2399 , http://hdl.handle.net/10962/d1002195
- Description: This thesis centres on the debates informing the progress of three public art galleries in South Africa between 1990 and 1994. This was a period of great change in the country, spanning from the unbanning of left-wing political parties and Nelson Mandela’s release from prison, to the first democratic elections which resulted in his inauguration as President of South Africa. The study focuses specifically on the Johannesburg Art Gallery, the South African National Gallery, and the Durban Art Gallery, delineating the events and exhibitions held, the programmes initiated, and the artists represented by these galleries during this post-apartheid/pre-democracy phase of the country’s history. The debates relevant to these galleries linked to those prevalent in the arts, museology, and politics at the time. Many contemporary South African artists called attention to apartheid oppression and human rights abuses during the 1980s. After 1990, with these pressures alleviating, there was a stage of uncertainty as to the role, responsibility, and focus of visual art in a post-‘struggle’ context, however there was also an unprecedented upswing in interest and investment in it. On a practical level, the administration of the arts was being re-evaluated and contested by both independent and politically-aligned arts groups. Public art museums and sponsored art competitions and exhibitions made increasing efforts to be ‘representative’ of South Africans of all races, cultures, creeds, sexes and genders. The many conferences, committees, and conventions created during this transitional era focused on the creation of policies that would assist in nation-building; historical and cultural redress and regeneration; and the education and representation of previously disadvantaged groups. This coincided with a revolution in museological discourses internationally, from the theorization of a museum as a place of commemoration and conservation, to a forum for discussion and revision between both academic and non-academic communities. With the sharing of the process of constructing history and knowledge, came the challenging dynamics involved in the representation of identity and history. In all of these groups - the arts, museology, and South African politics - the predominant issue seemed to be a negotiation between the bid to open up control to more parties, and the reluctance of some parties to relinquish control. While the emphasis is on significant changes that were implemented in the transitional period, the study locates the changes at the Johannesburg Art Gallery, the South African National Gallery and the Durban Art Gallery within their historical, geographical, and socio-political context. Various artists working in these locations during this era are also discussed, as the changes in their status, and the progressions in their subject matter, materials, and concerns are interesting to examine more nuanced definitions of the ‘political’, probing the politics of identity, sexuality, gender, race, geography, and belief systems. Some artists also focused specifically on post-apartheid preoccupations with territory, trauma, conflict, memory and freedom. This kind of artwork was assiduously acquired during the early ‘90s by public art galleries, whose exhibitions and collecting focus and policies were undergoing considerable revision and redress. This thesis examines these changes in light of their socio-political contexts, as well as in light of shifting national and international imperatives and conceptions of museums and museum practice.
- Full Text:
- Authors: Cook, Shashi Chailey
- Date: 2009
- Subjects: South African National Gallery Exhibitions Durban Art Gallery Exhibitions Joahnnesburg Art Gallery Exhibitions Art -- South Africa -- Exhibitions Art, South African Exhibitions South Africa History Exhibitions
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2399 , http://hdl.handle.net/10962/d1002195
- Description: This thesis centres on the debates informing the progress of three public art galleries in South Africa between 1990 and 1994. This was a period of great change in the country, spanning from the unbanning of left-wing political parties and Nelson Mandela’s release from prison, to the first democratic elections which resulted in his inauguration as President of South Africa. The study focuses specifically on the Johannesburg Art Gallery, the South African National Gallery, and the Durban Art Gallery, delineating the events and exhibitions held, the programmes initiated, and the artists represented by these galleries during this post-apartheid/pre-democracy phase of the country’s history. The debates relevant to these galleries linked to those prevalent in the arts, museology, and politics at the time. Many contemporary South African artists called attention to apartheid oppression and human rights abuses during the 1980s. After 1990, with these pressures alleviating, there was a stage of uncertainty as to the role, responsibility, and focus of visual art in a post-‘struggle’ context, however there was also an unprecedented upswing in interest and investment in it. On a practical level, the administration of the arts was being re-evaluated and contested by both independent and politically-aligned arts groups. Public art museums and sponsored art competitions and exhibitions made increasing efforts to be ‘representative’ of South Africans of all races, cultures, creeds, sexes and genders. The many conferences, committees, and conventions created during this transitional era focused on the creation of policies that would assist in nation-building; historical and cultural redress and regeneration; and the education and representation of previously disadvantaged groups. This coincided with a revolution in museological discourses internationally, from the theorization of a museum as a place of commemoration and conservation, to a forum for discussion and revision between both academic and non-academic communities. With the sharing of the process of constructing history and knowledge, came the challenging dynamics involved in the representation of identity and history. In all of these groups - the arts, museology, and South African politics - the predominant issue seemed to be a negotiation between the bid to open up control to more parties, and the reluctance of some parties to relinquish control. While the emphasis is on significant changes that were implemented in the transitional period, the study locates the changes at the Johannesburg Art Gallery, the South African National Gallery and the Durban Art Gallery within their historical, geographical, and socio-political context. Various artists working in these locations during this era are also discussed, as the changes in their status, and the progressions in their subject matter, materials, and concerns are interesting to examine more nuanced definitions of the ‘political’, probing the politics of identity, sexuality, gender, race, geography, and belief systems. Some artists also focused specifically on post-apartheid preoccupations with territory, trauma, conflict, memory and freedom. This kind of artwork was assiduously acquired during the early ‘90s by public art galleries, whose exhibitions and collecting focus and policies were undergoing considerable revision and redress. This thesis examines these changes in light of their socio-political contexts, as well as in light of shifting national and international imperatives and conceptions of museums and museum practice.
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Repetitive symbolic play as a therapeutic process
- Authors: Campbell, Megan
- Date: 2009
- Subjects: Play therapy Symbolic play Child psychology Developmental psychology
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2944 , http://hdl.handle.net/10962/d1002453
- Description: Child centred play therapy theory explains that the facilitative environment of the relationship between child and play therapist allows the child the opportunity to confront emotional pain imbedded in lived, relational experiences, and in so doing process and gain mastery over it. However very little research exists into how the child, through his use of repetitive, symbolic play, as a therapeutic process, achieves this resolution. This research project aims to address this gap in research by exploring and describing repetitive symbolic play as a therapeutic process within child centred play therapy that facilitates change in the child‟s sense of self, assisting him towards healthy adjustment. The research project uses a young boys‟ therapeutic process as a case study, employing a qualitative research design that draws from interpretative research. Fourteen repetitive play sequence themes were analysed within the context of the case formulation and follow-up feedback meetings that took place throughout the therapy process. Using a hermeneutic enquiry the researcher illustrates how the child‟s sense of self, dependent on his perceptions of his external environment, became far more congruent, as evident through his behaviour, when his environment, first in play therapy, then at home and at school became more consistent, supportive and nurturing. Within this facilitative environment the researcher then describes how the child used repetitive symbolic play to address and process emotional issues relating to earlier experiences within his external environment. Hermeneutic analysis suggests that the child‟s use of repetitive symbolic play within the facilitative relationship between him and his therapist became a therapeutic process in and of itself that contributed towards self-directed healing, and change within his sense of self, that promoted healthier adjustment within his environment.
- Full Text:
- Authors: Campbell, Megan
- Date: 2009
- Subjects: Play therapy Symbolic play Child psychology Developmental psychology
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2944 , http://hdl.handle.net/10962/d1002453
- Description: Child centred play therapy theory explains that the facilitative environment of the relationship between child and play therapist allows the child the opportunity to confront emotional pain imbedded in lived, relational experiences, and in so doing process and gain mastery over it. However very little research exists into how the child, through his use of repetitive, symbolic play, as a therapeutic process, achieves this resolution. This research project aims to address this gap in research by exploring and describing repetitive symbolic play as a therapeutic process within child centred play therapy that facilitates change in the child‟s sense of self, assisting him towards healthy adjustment. The research project uses a young boys‟ therapeutic process as a case study, employing a qualitative research design that draws from interpretative research. Fourteen repetitive play sequence themes were analysed within the context of the case formulation and follow-up feedback meetings that took place throughout the therapy process. Using a hermeneutic enquiry the researcher illustrates how the child‟s sense of self, dependent on his perceptions of his external environment, became far more congruent, as evident through his behaviour, when his environment, first in play therapy, then at home and at school became more consistent, supportive and nurturing. Within this facilitative environment the researcher then describes how the child used repetitive symbolic play to address and process emotional issues relating to earlier experiences within his external environment. Hermeneutic analysis suggests that the child‟s use of repetitive symbolic play within the facilitative relationship between him and his therapist became a therapeutic process in and of itself that contributed towards self-directed healing, and change within his sense of self, that promoted healthier adjustment within his environment.
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Restoration of degraded subtropical thickets in the Baviaanskloof Megareserve, South Africa: the role of carbon stocks and Portulacaria afra survivorship
- Authors: Powell, Michael John
- Date: 2009
- Subjects: Portulacaria afra -- South Africa -- Eastern Cape , Portulacaceae -- South Africa -- Eastern Cape , Biodiversity conservation -- South Africa -- Eastern Cape , Plant conservation -- South Africa -- Eastern Cape , Shrubs -- South Africa -- Eastern Cape , Succulent plants -- South Africa -- Eastern Cape , Baviaanskloof (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4764 , http://hdl.handle.net/10962/d1007164 , Portulacaria afra -- South Africa -- Eastern Cape , Portulacaceae -- South Africa -- Eastern Cape , Biodiversity conservation -- South Africa -- Eastern Cape , Plant conservation -- South Africa -- Eastern Cape , Shrubs -- South Africa -- Eastern Cape , Succulent plants -- South Africa -- Eastern Cape , Baviaanskloof (South Africa)
- Description: The semi-arid forms of subtropical thicket in the Eastern and Western Cape have been heavily degraded through unsustainable pastoralism over the last century or more. The degraded areas exhibit a significant loss of above-ground and belowground carbon stocks, and consequently provide an opportunity for restoration through the formal and informal carbon markets. A prerequisite for the attainment of carbon credits is to ensure sound carbon stock baselines prior to effecting restoration. I report on the carbon stocks (including sub-pools) for a number of intact subtropical thicket types, as well as the differentials between the intact and degraded states (including the sub-pools). Total carbon stocks (TCS in t C ha⁻¹) for intact vegetation (to a soil depth 0–25 cm), ranged from 87.73±6.51 to 70.64±17.24. For degraded vegetation (including old lands), TCS (t C ha⁻¹) ranged from 34.05±3.61 to 21.03±2.70. For all vegetation types, the differentials in TCS along the degradation gradient (0–25 cm) are highly significant and strengthen the possibility for carbon credit financing to catalyse the restoration of the degraded semi-arid subtropical thickets. This study has shown a mean loss of 57.23 t C ha⁻¹ in Baviaanskloof spekboom thickets, when measured to a soil depth of 25 cm. Portulacaria afra is a key species within the semi-arid subtropical thickets, being a canopy dominant and a driver of soil nutrient status, but has been largely lost from the degraded landscapes. Degraded semi-arid subtropical thicket vegetation lacks regeneration via seedling recruitment; restoration therefore requires the manual replanting of P. afra using cut truncheons. Survivorship trials were undertaken infield in 2005 to establish restoration protocols for P. afra, with survivorship being recorded in 2006 and 2008. Overall survivorship for all treatments was found to be 43.2±2.8% in 2006, dropping to 35.8±2.7% in 2008. Planting posture (flat or upright) showed the most significant results of all the factors tested in 2006 and 2008. Micro-damming also influenced survivorship in that micro-damming was associated with marginally higher survivorship (47.4 ±2.0% with damming vs. 39.1±1.5% without damming in 2006, and 39.3±1.9% vs. 32.3±1.5% in 2008). Higher planting density only showed a significant positive impact on survivorship in 2008. Neither stem diameter nor clumping significantly affected degree of survival. A key finding in the study has been the non-static nature of P. afra truncheon survivorship, even after being well established (three years since planting). The results from the study will guide the restoration protocols for the restoration of degraded subtropical thickets, where P. afra requires replanting.
- Full Text:
- Authors: Powell, Michael John
- Date: 2009
- Subjects: Portulacaria afra -- South Africa -- Eastern Cape , Portulacaceae -- South Africa -- Eastern Cape , Biodiversity conservation -- South Africa -- Eastern Cape , Plant conservation -- South Africa -- Eastern Cape , Shrubs -- South Africa -- Eastern Cape , Succulent plants -- South Africa -- Eastern Cape , Baviaanskloof (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4764 , http://hdl.handle.net/10962/d1007164 , Portulacaria afra -- South Africa -- Eastern Cape , Portulacaceae -- South Africa -- Eastern Cape , Biodiversity conservation -- South Africa -- Eastern Cape , Plant conservation -- South Africa -- Eastern Cape , Shrubs -- South Africa -- Eastern Cape , Succulent plants -- South Africa -- Eastern Cape , Baviaanskloof (South Africa)
- Description: The semi-arid forms of subtropical thicket in the Eastern and Western Cape have been heavily degraded through unsustainable pastoralism over the last century or more. The degraded areas exhibit a significant loss of above-ground and belowground carbon stocks, and consequently provide an opportunity for restoration through the formal and informal carbon markets. A prerequisite for the attainment of carbon credits is to ensure sound carbon stock baselines prior to effecting restoration. I report on the carbon stocks (including sub-pools) for a number of intact subtropical thicket types, as well as the differentials between the intact and degraded states (including the sub-pools). Total carbon stocks (TCS in t C ha⁻¹) for intact vegetation (to a soil depth 0–25 cm), ranged from 87.73±6.51 to 70.64±17.24. For degraded vegetation (including old lands), TCS (t C ha⁻¹) ranged from 34.05±3.61 to 21.03±2.70. For all vegetation types, the differentials in TCS along the degradation gradient (0–25 cm) are highly significant and strengthen the possibility for carbon credit financing to catalyse the restoration of the degraded semi-arid subtropical thickets. This study has shown a mean loss of 57.23 t C ha⁻¹ in Baviaanskloof spekboom thickets, when measured to a soil depth of 25 cm. Portulacaria afra is a key species within the semi-arid subtropical thickets, being a canopy dominant and a driver of soil nutrient status, but has been largely lost from the degraded landscapes. Degraded semi-arid subtropical thicket vegetation lacks regeneration via seedling recruitment; restoration therefore requires the manual replanting of P. afra using cut truncheons. Survivorship trials were undertaken infield in 2005 to establish restoration protocols for P. afra, with survivorship being recorded in 2006 and 2008. Overall survivorship for all treatments was found to be 43.2±2.8% in 2006, dropping to 35.8±2.7% in 2008. Planting posture (flat or upright) showed the most significant results of all the factors tested in 2006 and 2008. Micro-damming also influenced survivorship in that micro-damming was associated with marginally higher survivorship (47.4 ±2.0% with damming vs. 39.1±1.5% without damming in 2006, and 39.3±1.9% vs. 32.3±1.5% in 2008). Higher planting density only showed a significant positive impact on survivorship in 2008. Neither stem diameter nor clumping significantly affected degree of survival. A key finding in the study has been the non-static nature of P. afra truncheon survivorship, even after being well established (three years since planting). The results from the study will guide the restoration protocols for the restoration of degraded subtropical thickets, where P. afra requires replanting.
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Restructuring of the Port Elizabeth Hospital Complex: a perspective from the planned change management approach
- Authors: Qwesha, Babalwa
- Date: 2009
- Subjects: Hospitals -- Administration -- South Africa -- Port Elizabeth Organizational change -- Management -- South Africa Case studies
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:762 , http://hdl.handle.net/10962/d1003883
- Description: The research objectives which underpin this study were threefold. Firstly was to analyze the Port Elizabeth Hospital Complex (PEHC) restructuring process from a planned changed management perspective in particular the three stage model of Lewin (1951) which include unfreezing the current equilibrium, moving to a new position and refreezing in the new position. Secondly was to analyze how unforeseen circumstances were dealt with. Thirdly was to analyse the setting of objectives and measurements of targets to monitor progress. The study is based on the restructuring that took place in the PEHC which was called “Rationalization”. The research indicates that the development and implementation of the rationalization cannot be understood from the perspective of the three stage model of Lewin (1951). The conclusion was based on the manager’s perceptions of their analysis of the restructuring in the light of the theory of the three stage model of Lewin (1951). The study has shown that: · Rationalisation began by gathering information on the shortcomings of the structure of the three hospitals, but did not understand the degree of readiness to change. · The timescales for achieving rationalization were not clearly defined. · It was driven from the top with clear objectives and no timescales. · There was no structure that prepared the employees to go through the process of rationalisation. · There was lack of capacity of middle managers to respond to the workers in an encouraging way. · Rationalisation sub-committees had limited time to meet with employees at the sectional level. · External stakeholder involvement was not mobilized to its full potential. · Rationalisation was not an open process that involved both formal and informal employees. · Budget constraints and staff shortages were not informed by the restructuring needs. · Workers did not feel secure about the current and future work practises. · There was no feeling that the change will be beneficial to their wellbeing and to the organisation’s goals and mission · There was no monitoring and evaluation put in place to track progress. · There was centralisation of even the most basic administrative functions. The study seems to imply that the restructuring in the PEHC bears no resemblance to the Lewin (1951) planned change model and therefore cannot be understood from the perspective of this model. Given the initiative to rationalize, the PEHC management can learn from the model of Lewin (1951) planned change.
- Full Text:
- Authors: Qwesha, Babalwa
- Date: 2009
- Subjects: Hospitals -- Administration -- South Africa -- Port Elizabeth Organizational change -- Management -- South Africa Case studies
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:762 , http://hdl.handle.net/10962/d1003883
- Description: The research objectives which underpin this study were threefold. Firstly was to analyze the Port Elizabeth Hospital Complex (PEHC) restructuring process from a planned changed management perspective in particular the three stage model of Lewin (1951) which include unfreezing the current equilibrium, moving to a new position and refreezing in the new position. Secondly was to analyze how unforeseen circumstances were dealt with. Thirdly was to analyse the setting of objectives and measurements of targets to monitor progress. The study is based on the restructuring that took place in the PEHC which was called “Rationalization”. The research indicates that the development and implementation of the rationalization cannot be understood from the perspective of the three stage model of Lewin (1951). The conclusion was based on the manager’s perceptions of their analysis of the restructuring in the light of the theory of the three stage model of Lewin (1951). The study has shown that: · Rationalisation began by gathering information on the shortcomings of the structure of the three hospitals, but did not understand the degree of readiness to change. · The timescales for achieving rationalization were not clearly defined. · It was driven from the top with clear objectives and no timescales. · There was no structure that prepared the employees to go through the process of rationalisation. · There was lack of capacity of middle managers to respond to the workers in an encouraging way. · Rationalisation sub-committees had limited time to meet with employees at the sectional level. · External stakeholder involvement was not mobilized to its full potential. · Rationalisation was not an open process that involved both formal and informal employees. · Budget constraints and staff shortages were not informed by the restructuring needs. · Workers did not feel secure about the current and future work practises. · There was no feeling that the change will be beneficial to their wellbeing and to the organisation’s goals and mission · There was no monitoring and evaluation put in place to track progress. · There was centralisation of even the most basic administrative functions. The study seems to imply that the restructuring in the PEHC bears no resemblance to the Lewin (1951) planned change model and therefore cannot be understood from the perspective of this model. Given the initiative to rationalize, the PEHC management can learn from the model of Lewin (1951) planned change.
- Full Text:
Rights, duties and remedies under the United Nations Convention on Contracts for the International Sale of Goods: an investigation into the CISG's compatibility with South African law
- Authors: Oosthuizen, Beverley-Claire
- Date: 2009
- Subjects: United Nations Convention on Contracts for the International Sale of Goods (1980) , Sales -- South Africa , Commercial law -- South Africa , South Africa -- Law and legislation
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:3691 , http://hdl.handle.net/10962/d1003206 , United Nations Convention on Contracts for the International Sale of Goods (1980) , Sales -- South Africa , Commercial law -- South Africa , South Africa -- Law and legislation
- Description: This thesis analyses the compatibility of the United Nations Convention on Contracts for the International Sale of Goods (CISG) with the South African law of sale. An initial examination of the historical development of the CISG reveals its ambitions of becoming the primary source of law governing international contracts of sale. The goal of this research is to determine whether South Africa should ratify the CISG. The CISG has been ratified by most of the leading trading States in the world. In order to gain a better understanding of the advantages and disadvantages of ratification, a comparative study has been undertaken. The stance taken toward the CISG by the United Kingdom and Germany has been examined. The United Kingdom has staunchly avoided ratifying the CISG, despite having agreed thereto a number of years ago. Germany however has taken a different approach and has welcomed the CISG. The experiences of these foreign States serve as a useful guide when assessing the specific challenges that exist in South Africa concerning the adoption of the CISG. The most important aspect of this study is the direct comparison between the legal provisions housed in the CISG and their counterparts under South African law. A careful investigation has been conducted into the rights, duties, and remedies under the CISG. This investigation is followed by an examination of the corresponding rights, duties, and remedies under the South African domestic law of sale. It is evident from these explorations that the rights and duties under the CISG strongly resemble those under South African law. The direct comparison revealed however that certain remedies found in the CISG do not have a counterpart under South African law. Despite this discrepancy, there are no legal principles in the CISG that are completely unknown in South African law. While certain remedies housed in the CISG cannot be found in an identical form under South African law, sufficiently similar legal principles can be found, which frequently lead to the same results as those under the CISG. This study is concluded with a recommendation concerning South Africa’s adoption of the CISG.
- Full Text:
- Authors: Oosthuizen, Beverley-Claire
- Date: 2009
- Subjects: United Nations Convention on Contracts for the International Sale of Goods (1980) , Sales -- South Africa , Commercial law -- South Africa , South Africa -- Law and legislation
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:3691 , http://hdl.handle.net/10962/d1003206 , United Nations Convention on Contracts for the International Sale of Goods (1980) , Sales -- South Africa , Commercial law -- South Africa , South Africa -- Law and legislation
- Description: This thesis analyses the compatibility of the United Nations Convention on Contracts for the International Sale of Goods (CISG) with the South African law of sale. An initial examination of the historical development of the CISG reveals its ambitions of becoming the primary source of law governing international contracts of sale. The goal of this research is to determine whether South Africa should ratify the CISG. The CISG has been ratified by most of the leading trading States in the world. In order to gain a better understanding of the advantages and disadvantages of ratification, a comparative study has been undertaken. The stance taken toward the CISG by the United Kingdom and Germany has been examined. The United Kingdom has staunchly avoided ratifying the CISG, despite having agreed thereto a number of years ago. Germany however has taken a different approach and has welcomed the CISG. The experiences of these foreign States serve as a useful guide when assessing the specific challenges that exist in South Africa concerning the adoption of the CISG. The most important aspect of this study is the direct comparison between the legal provisions housed in the CISG and their counterparts under South African law. A careful investigation has been conducted into the rights, duties, and remedies under the CISG. This investigation is followed by an examination of the corresponding rights, duties, and remedies under the South African domestic law of sale. It is evident from these explorations that the rights and duties under the CISG strongly resemble those under South African law. The direct comparison revealed however that certain remedies found in the CISG do not have a counterpart under South African law. Despite this discrepancy, there are no legal principles in the CISG that are completely unknown in South African law. While certain remedies housed in the CISG cannot be found in an identical form under South African law, sufficiently similar legal principles can be found, which frequently lead to the same results as those under the CISG. This study is concluded with a recommendation concerning South Africa’s adoption of the CISG.
- Full Text:
Salinity induced physiological responses in juvenile dusky kob, Argyrosomus japonicus (Sciaenidae)
- Authors: Bernatzeder, Andrea Katinka
- Date: 2009
- Subjects: Osmoregulation , Argyrosomus -- Effects of salt on -- South Africa , Sciaenidae -- Effects of salt on -- South Africa , Argyrosomus -- Physiology , Sciaenidae -- Physiology , Fishes -- Physiology , Biological control systems , Marine ecology -- South Africa , Fishery management -- South Africa , Mariculture -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5318 , http://hdl.handle.net/10962/d1005163 , Osmoregulation , Argyrosomus -- Effects of salt on -- South Africa , Sciaenidae -- Effects of salt on -- South Africa , Argyrosomus -- Physiology , Sciaenidae -- Physiology , Fishes -- Physiology , Biological control systems , Marine ecology -- South Africa , Fishery management -- South Africa , Mariculture -- South Africa
- Description: Fisheries management regulations for dusky kob Argyrosomus japonicus, an important commercial and recreational fisheries species, have failed and the stock is considered collapsed. It is important to take an ecosystems approach to management which includes understanding the effect of environmental factors on recruitment, abundance and distribution. The distribution of early juveniles (20-150 mm TL) in the wild appears to be restricted to the upper reaches of estuaries at salinities below 5 psu. Food availability could not explain the distribution of early juveniles. The aim of this study was to investigate the role of salinity on the distribution of early juvenile dusky kob (<150 mm TL) by examining physiological responses of juveniles exposed to a range of salinities under laboratory conditions. The hypothesis was that the physiological functioning of early juveniles would be optimised at the reduced salinities which they naturally occur at. The objectives of this study were to investigate the effect of salinity on: i) plasma osmolality; ii) growth, food conversion ratio and condition factor; and iii) gill histology with emphasis on chloride cell size and number. A preliminary study was undertaken to determine whether the use of 2-phenoxyethanol had an effect on plasma osmolality. Juveniles pithed prior to blood sampling were used as the control. Plasma osmolality was not affected by exposure or duration of exposure (2, 4, 6, 8, 10 min) to 2-phenoxyethanol. The ability of teleosts to regulate plasma osmolality over a wide range of salinities indicates their degree of ‘physiological euryhalinity’. Plasma osmolality of juveniles exposed to 5, 12 and 35 psu was measured every two weeks over a total of six weeks. Although juveniles were able to regulate plasma osmolality over the duration of the experiment, plasma osmolality at 5 and 12 psu was significantly lower than in fish maintained at 35 psu. Growth is used as an indicator of the relative energy used for osmoregulation at different salinities, as the energy used for osmoregulation becomes unavailable for growth. A nineweek growth experiment was conducted on juveniles exposed to 5, 12 and 35 psu. Juveniles grew and survived at all three salinities. However, growth of juveniles at 5 psu was significantly lower than at 12 and 35 psu. Other than a significantly greater weight gain at 35 psu relative to 12 psu, there was no significant difference in specific growth and length gain between juveniles at 12 and 35 psu. Food conversion ratio and condition factor at 12 and 35 psu were not significantly different, but food conversion ratio and condition factor at 5 psu was significantly greater and lower than at 35 psu respectively. In fish, gills are considered the major organ involved in osmoregulation. Within the gills, chloride cells are the predominant site of ion exchange which is driven by the Na⁺, K⁺- ATPase enzyme. Gill samples of juveniles exposed to 5, 12 and 35 psu for six weeks were examined histologically using light microscopy. Chloride cells of juveniles maintained at 5 psu were significantly more abundant than in juveniles at 12 and 35 psu. Chloride cells of juveniles at 5 psu were significantly larger than in juveniles kept at 12 psu, but not significantly different to those of juveniles kept at 35 psu. The ability of the juvenile fish to regulate plasma osmolality indicates that they are 'physiologically euryhaline', but the reduced growth and proliferation of chloride cells at 5 psu suggests that energy expenditure for osmoregulation is increased at hypoosmotic salinities. Salinity induced physiological responses could therefore not explain the natural distribution of early juvenile dusky kob and it is proposed that other environmental factors (e.g. temperature) are also important. It is also hypothesised that the high conductivity of an estuary in South Africa, to which our understanding is limited, may negate the effect of reduced salinity. Although freshwater input into estuaries is an important factor, further investigations to explain the distribution and abundance of early juveniles is required to make management recommendations. Dusky kob is also becoming an increasingly popular aquaculture species in South Africa. In this regard, early juvenile dusky kob can be grown at salinities as low as 12 psu without negatively affecting growth and production.
- Full Text:
- Authors: Bernatzeder, Andrea Katinka
- Date: 2009
- Subjects: Osmoregulation , Argyrosomus -- Effects of salt on -- South Africa , Sciaenidae -- Effects of salt on -- South Africa , Argyrosomus -- Physiology , Sciaenidae -- Physiology , Fishes -- Physiology , Biological control systems , Marine ecology -- South Africa , Fishery management -- South Africa , Mariculture -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5318 , http://hdl.handle.net/10962/d1005163 , Osmoregulation , Argyrosomus -- Effects of salt on -- South Africa , Sciaenidae -- Effects of salt on -- South Africa , Argyrosomus -- Physiology , Sciaenidae -- Physiology , Fishes -- Physiology , Biological control systems , Marine ecology -- South Africa , Fishery management -- South Africa , Mariculture -- South Africa
- Description: Fisheries management regulations for dusky kob Argyrosomus japonicus, an important commercial and recreational fisheries species, have failed and the stock is considered collapsed. It is important to take an ecosystems approach to management which includes understanding the effect of environmental factors on recruitment, abundance and distribution. The distribution of early juveniles (20-150 mm TL) in the wild appears to be restricted to the upper reaches of estuaries at salinities below 5 psu. Food availability could not explain the distribution of early juveniles. The aim of this study was to investigate the role of salinity on the distribution of early juvenile dusky kob (<150 mm TL) by examining physiological responses of juveniles exposed to a range of salinities under laboratory conditions. The hypothesis was that the physiological functioning of early juveniles would be optimised at the reduced salinities which they naturally occur at. The objectives of this study were to investigate the effect of salinity on: i) plasma osmolality; ii) growth, food conversion ratio and condition factor; and iii) gill histology with emphasis on chloride cell size and number. A preliminary study was undertaken to determine whether the use of 2-phenoxyethanol had an effect on plasma osmolality. Juveniles pithed prior to blood sampling were used as the control. Plasma osmolality was not affected by exposure or duration of exposure (2, 4, 6, 8, 10 min) to 2-phenoxyethanol. The ability of teleosts to regulate plasma osmolality over a wide range of salinities indicates their degree of ‘physiological euryhalinity’. Plasma osmolality of juveniles exposed to 5, 12 and 35 psu was measured every two weeks over a total of six weeks. Although juveniles were able to regulate plasma osmolality over the duration of the experiment, plasma osmolality at 5 and 12 psu was significantly lower than in fish maintained at 35 psu. Growth is used as an indicator of the relative energy used for osmoregulation at different salinities, as the energy used for osmoregulation becomes unavailable for growth. A nineweek growth experiment was conducted on juveniles exposed to 5, 12 and 35 psu. Juveniles grew and survived at all three salinities. However, growth of juveniles at 5 psu was significantly lower than at 12 and 35 psu. Other than a significantly greater weight gain at 35 psu relative to 12 psu, there was no significant difference in specific growth and length gain between juveniles at 12 and 35 psu. Food conversion ratio and condition factor at 12 and 35 psu were not significantly different, but food conversion ratio and condition factor at 5 psu was significantly greater and lower than at 35 psu respectively. In fish, gills are considered the major organ involved in osmoregulation. Within the gills, chloride cells are the predominant site of ion exchange which is driven by the Na⁺, K⁺- ATPase enzyme. Gill samples of juveniles exposed to 5, 12 and 35 psu for six weeks were examined histologically using light microscopy. Chloride cells of juveniles maintained at 5 psu were significantly more abundant than in juveniles at 12 and 35 psu. Chloride cells of juveniles at 5 psu were significantly larger than in juveniles kept at 12 psu, but not significantly different to those of juveniles kept at 35 psu. The ability of the juvenile fish to regulate plasma osmolality indicates that they are 'physiologically euryhaline', but the reduced growth and proliferation of chloride cells at 5 psu suggests that energy expenditure for osmoregulation is increased at hypoosmotic salinities. Salinity induced physiological responses could therefore not explain the natural distribution of early juvenile dusky kob and it is proposed that other environmental factors (e.g. temperature) are also important. It is also hypothesised that the high conductivity of an estuary in South Africa, to which our understanding is limited, may negate the effect of reduced salinity. Although freshwater input into estuaries is an important factor, further investigations to explain the distribution and abundance of early juveniles is required to make management recommendations. Dusky kob is also becoming an increasingly popular aquaculture species in South Africa. In this regard, early juvenile dusky kob can be grown at salinities as low as 12 psu without negatively affecting growth and production.
- Full Text:
School grounds as a place for environmental learning in the life skills learning programme
- Authors: Mambinja, Sindiswa
- Date: 2009
- Subjects: Environmental education -- Study and teaching (Elementary) -- South Africa --Grahamstown Life skills -- Study and teaching (Elementary) -- South Africa Education -- South Africa -- Curricula Education, Elementary -- South Africa School grounds -- South Africa -- Grahamstown Curriculum-based assessment -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1757 , http://hdl.handle.net/10962/d1003642
- Description: With the intention of improving my own practice, the study investigated how school grounds could be used for environmental learning in the Foundation Phase Life Skill Learning Programme within the Revised National Curriculum Statement. The research was conducted in the Grade One class of Ntaba Maria Primary School situated in Grahamstown East. The study was an action research case study based on interpretive approach to research. It had two cycles comprised of two lesson plans. The first Lesson Plan focused on an audit of the school grounds to identify environmental issues. The second Lesson Plan was informed by the audit, and addressed one of the identified environmental issues. Data was gathered through analysis of curriculum documents, video recordings and photographs of learning interactions, journal entries and learners’ work. The study highlighted that school grounds provided a context for environmental learning within Life Orientation Learning Area especially when integrated with other learning areas. There were cases however, where assessment standards were only partly addressed. The study also revealed that the Life Orientation Learning Area was also limiting for school grounds learning opportunities in some cases. In other cases the study highlighted the open-ended nature of some of its assessment standards. The study also revealed how school grounds related activities offered opportunities and challenges for constructivist, situated and active learning. Opportunities and challenges regarding group work, language use, active engagement with information learning in authentic settings, and action and action competence; emerged as issues from the analysis. Finally, the study shed light on how reflection on learners’ work and assessment of these could help teachers modify activities and do them differently so as to enhance the achievement of skills, knowledge, attitudes and values. The achievement of investigative and problem-solving skills through the two lesson plans was critically evaluated as the processes of values education employed in the lesson implementation.
- Full Text:
- Authors: Mambinja, Sindiswa
- Date: 2009
- Subjects: Environmental education -- Study and teaching (Elementary) -- South Africa --Grahamstown Life skills -- Study and teaching (Elementary) -- South Africa Education -- South Africa -- Curricula Education, Elementary -- South Africa School grounds -- South Africa -- Grahamstown Curriculum-based assessment -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1757 , http://hdl.handle.net/10962/d1003642
- Description: With the intention of improving my own practice, the study investigated how school grounds could be used for environmental learning in the Foundation Phase Life Skill Learning Programme within the Revised National Curriculum Statement. The research was conducted in the Grade One class of Ntaba Maria Primary School situated in Grahamstown East. The study was an action research case study based on interpretive approach to research. It had two cycles comprised of two lesson plans. The first Lesson Plan focused on an audit of the school grounds to identify environmental issues. The second Lesson Plan was informed by the audit, and addressed one of the identified environmental issues. Data was gathered through analysis of curriculum documents, video recordings and photographs of learning interactions, journal entries and learners’ work. The study highlighted that school grounds provided a context for environmental learning within Life Orientation Learning Area especially when integrated with other learning areas. There were cases however, where assessment standards were only partly addressed. The study also revealed that the Life Orientation Learning Area was also limiting for school grounds learning opportunities in some cases. In other cases the study highlighted the open-ended nature of some of its assessment standards. The study also revealed how school grounds related activities offered opportunities and challenges for constructivist, situated and active learning. Opportunities and challenges regarding group work, language use, active engagement with information learning in authentic settings, and action and action competence; emerged as issues from the analysis. Finally, the study shed light on how reflection on learners’ work and assessment of these could help teachers modify activities and do them differently so as to enhance the achievement of skills, knowledge, attitudes and values. The achievement of investigative and problem-solving skills through the two lesson plans was critically evaluated as the processes of values education employed in the lesson implementation.
- Full Text:
Secondary school learners' perceptions of the value of integrating ICT into the curriculum: an exploratory study in the Grahamstown circuit
- Authors: Mbane, Nombeko Precious
- Date: 2009
- Subjects: Educational technology -- South Africa Information technology -- South Africa Education, Secondary -- Curricula -- South Africa Computer-assisted instruction -- South Africa Educational innovations Information technology -- Study and teaching (Secondary) -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1762 , http://hdl.handle.net/10962/d1003647
- Description: According to the Draft White Paper on e-Education (DoE, 2003), the objective of ICTs in education is to build digital and information literacy so that all learners become confident and competent in using technology to contribute to an innovative and developing South African society. International researchers have acknowledged that little is known about learners’ perceptions of their own learning, but that learners are aware of how technology can be used and have the potential to contribute ideas about ICTs can be used to support their learning. To add to this growing body of research, this study sought to establish secondary school learners’ experiences and perceptions of the value of integrating ICT into the curriculum and the extent to which they believe that ICT is meaningfully integrated into the curriculum. Although some of the schools do not have the necessary ICT infrastructure, this did not preclude the learners from having opinions about the potential benefits and drawbacks of ICT. The findings display the learners’ perceptions on ICT use within the curriculum and how this relates to the available infrastructure in schools.
- Full Text:
- Authors: Mbane, Nombeko Precious
- Date: 2009
- Subjects: Educational technology -- South Africa Information technology -- South Africa Education, Secondary -- Curricula -- South Africa Computer-assisted instruction -- South Africa Educational innovations Information technology -- Study and teaching (Secondary) -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1762 , http://hdl.handle.net/10962/d1003647
- Description: According to the Draft White Paper on e-Education (DoE, 2003), the objective of ICTs in education is to build digital and information literacy so that all learners become confident and competent in using technology to contribute to an innovative and developing South African society. International researchers have acknowledged that little is known about learners’ perceptions of their own learning, but that learners are aware of how technology can be used and have the potential to contribute ideas about ICTs can be used to support their learning. To add to this growing body of research, this study sought to establish secondary school learners’ experiences and perceptions of the value of integrating ICT into the curriculum and the extent to which they believe that ICT is meaningfully integrated into the curriculum. Although some of the schools do not have the necessary ICT infrastructure, this did not preclude the learners from having opinions about the potential benefits and drawbacks of ICT. The findings display the learners’ perceptions on ICT use within the curriculum and how this relates to the available infrastructure in schools.
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Sending and receiving: immunity sought by diplomats committing criminal offences
- Authors: Moutzouris, Maria
- Date: 2009
- Subjects: Diplomatic privileges and immunities , International crimes , Criminal law , International law
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:3686 , http://hdl.handle.net/10962/d1003201 , Diplomatic privileges and immunities , International crimes , Criminal law , International law
- Description: Diplomatic immunity is one of the oldest elements of foreign relations, dating back as far as Ancient Greece and Rome. Today, it is a principle that has been codified into the Vienna Convention on Diplomatic Relations regulating past customs and practices. Consuls and international organizations, although their privileges and immunities are similar to diplomatic personnel, do differ and are regulated by the Vienna Convention on Consular Relations and the United Nations International Immunities respectively. These Conventions have been influenced by past practices and by three theories during different era’s namely exterritoriality, personal representation and functional necessity. The Vienna Convention on Diplomatic Relations further provides certain immunities and privileges to different levels of diplomatic officials, their staff and families. Privileges and immunities will be considered under various main categories, namely the diplomatic mission, the diplomatic official, diplomatic staff, and families. Each category receives privileges and immunities, for example immunities enjoyed by the diplomatic mission include mission correspondence and bags. Diplomatic officials enjoy personal inviolability, immunity from jurisdiction and inviolability of diplomats’ residences and property. The staff and families of diplomatic officials too enjoy privileges and immunities. The problem of so many people receiving privileges and immunities is that there is a high likelihood of abuse. Abuses that arise are various crimes committed by diplomats, their staff and families. They are immune from local punishment and appear to be above the local law. Although the Vienna Convention on Diplomatic Relations provides remedies against diplomats, staff and families who abuse their position, it gives the impression that it is not enough. Various Acts in the United Kingdom, United States and the Republic of South Africa will be analysed in order to ascertain what governments have done to try and curb diplomatic abuses. Each will be considered and found that although they have restricted immunity from previous practices it still places the diplomats’ needs above its own citizens. Thus several suggestions have been put forward and argued whether they are successful in restricting immunity comprehensively. Such suggestions are amending the Vienna Convention on Diplomatic Relations; using the functional necessity theory to further limit immunity; forming bilateral treaties between States as a possible means to restrict or limit; and lastly establishing a Permanent International Diplomatic Criminal Court. The key question to be answered is whether diplomatic immunity is needed for the efficient functioning of foreign relations between States.
- Full Text:
- Authors: Moutzouris, Maria
- Date: 2009
- Subjects: Diplomatic privileges and immunities , International crimes , Criminal law , International law
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:3686 , http://hdl.handle.net/10962/d1003201 , Diplomatic privileges and immunities , International crimes , Criminal law , International law
- Description: Diplomatic immunity is one of the oldest elements of foreign relations, dating back as far as Ancient Greece and Rome. Today, it is a principle that has been codified into the Vienna Convention on Diplomatic Relations regulating past customs and practices. Consuls and international organizations, although their privileges and immunities are similar to diplomatic personnel, do differ and are regulated by the Vienna Convention on Consular Relations and the United Nations International Immunities respectively. These Conventions have been influenced by past practices and by three theories during different era’s namely exterritoriality, personal representation and functional necessity. The Vienna Convention on Diplomatic Relations further provides certain immunities and privileges to different levels of diplomatic officials, their staff and families. Privileges and immunities will be considered under various main categories, namely the diplomatic mission, the diplomatic official, diplomatic staff, and families. Each category receives privileges and immunities, for example immunities enjoyed by the diplomatic mission include mission correspondence and bags. Diplomatic officials enjoy personal inviolability, immunity from jurisdiction and inviolability of diplomats’ residences and property. The staff and families of diplomatic officials too enjoy privileges and immunities. The problem of so many people receiving privileges and immunities is that there is a high likelihood of abuse. Abuses that arise are various crimes committed by diplomats, their staff and families. They are immune from local punishment and appear to be above the local law. Although the Vienna Convention on Diplomatic Relations provides remedies against diplomats, staff and families who abuse their position, it gives the impression that it is not enough. Various Acts in the United Kingdom, United States and the Republic of South Africa will be analysed in order to ascertain what governments have done to try and curb diplomatic abuses. Each will be considered and found that although they have restricted immunity from previous practices it still places the diplomats’ needs above its own citizens. Thus several suggestions have been put forward and argued whether they are successful in restricting immunity comprehensively. Such suggestions are amending the Vienna Convention on Diplomatic Relations; using the functional necessity theory to further limit immunity; forming bilateral treaties between States as a possible means to restrict or limit; and lastly establishing a Permanent International Diplomatic Criminal Court. The key question to be answered is whether diplomatic immunity is needed for the efficient functioning of foreign relations between States.
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Shame is valuable
- Authors: Euvrard, Jonathan George
- Date: 2009
- Subjects: Shame Ethics Self-evaluation
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2708 , http://hdl.handle.net/10962/d1002838
- Description: In this thesis I argue that shame is valuable because it contributes to the moral life by promoting coherence. I start by developing and defending a conception of shame. On my conception, rational shame involves a negative self-assessment, in which I am both the assessor and the object of assessment, and in which the standard of assessment is my own. I then develop a notion of coherence, and apply it to the relationship between values, and the relationship between values and actions. I also tie the notion of coherence to what I call “the moral life”. I then discuss two ways in which shame can work to promote coherence. Firstly, I describe a process of critical reflective self-assessment, and show how this is a particularly effective method of promoting coherence when coupled with shame. Secondly, I discuss the connection between my emotions and my values, arguing that this connection promotes coherence, and that shame works to reinforce this connection and thereby to promote coherence.
- Full Text:
- Authors: Euvrard, Jonathan George
- Date: 2009
- Subjects: Shame Ethics Self-evaluation
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2708 , http://hdl.handle.net/10962/d1002838
- Description: In this thesis I argue that shame is valuable because it contributes to the moral life by promoting coherence. I start by developing and defending a conception of shame. On my conception, rational shame involves a negative self-assessment, in which I am both the assessor and the object of assessment, and in which the standard of assessment is my own. I then develop a notion of coherence, and apply it to the relationship between values, and the relationship between values and actions. I also tie the notion of coherence to what I call “the moral life”. I then discuss two ways in which shame can work to promote coherence. Firstly, I describe a process of critical reflective self-assessment, and show how this is a particularly effective method of promoting coherence when coupled with shame. Secondly, I discuss the connection between my emotions and my values, arguing that this connection promotes coherence, and that shame works to reinforce this connection and thereby to promote coherence.
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Social learning processes of HIV/AIDS women caregivers on their use of traditional foods and medicinal plants : the case of Raphael Centre and Keiskamma Art and Health Centre communities of practice, Eastern Cape Province, South Africa
- Authors: Shonhai, Venencia F
- Date: 2009
- Subjects: Keiskamma Art Project (South Africa) , Women caregivers -- South Africa -- Eastern Cape , HIV infections -- Study and teaching -- South Africa , AIDS (Disease) -- Study and teaching -- South Africa , HIV-positive persons -- Care -- South Africa -- Eastern Cape , Medicinal plants -- South Africa , Natural foods -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1768 , http://hdl.handle.net/10962/d1003653
- Description: The scale of people being infected by HIV/AIDS (Human Immunodeficiency Virus /Acquired Immune Deficiency Syndrome) has meant that the family and the community have had to become involved in caring for the sick (Van Dyk, 2005). This has inevitably led to the emergence of informal caregivers in the form of family members caring for their relatives (Kipp, Nkosi, Laing & Jhangri, 2006). The research investigated the social learning of women caregivers looking after people living with HIV/AIDS, with emphasis on caregiving practices related to how they use traditional foods and medicinal plants. The research was undertaken in Grahamstown at the Raphael Centre and in Hamburg at Keiskkamma Health Centre and Art Project, Eastern Cape, South Africa. Data was collected using interviews, focus group discussions and diaries written by participants. The data was analyzed in two phases: the first phase involved reading the interview transcripts and collating the responses into analytical memos that were captured into broad categories, while the second phase made use of the community of practice analytical framework to further analyze the data to get better understanding of the social learning processes. This study reveals that participating in a community of practice like Raphael Centre and Keiskamma Health Centre enables caregivers to learn about caregiving. It also reveals that within these communities of practice there are varied learning processes that take place, such as observational and collaborative learning. The research also revealed that caregivers learn from the communities from which they come, for example caregivers learn about traditional food and medicinal plants which they use from their family members, friends, other caregivers as well as non governmental organizations. The research found that caregivers are influenced in their learning and practices by a number of factors which include their own experiences, ambivalent messages from different stakeholders concerned with fighting HIV/AIDS and exposure to new information. The research recommends that diverse learning processes in a community of practice and outside a community of practice should be encouraged and strengthened. It also recommends that HIV/AIDS caregiving options should be strengthened by drawing on experience and knowledge of caregivers. Caregivers should be encouraged to be selfsustaining to improve their caregiving practices. Stakeholders in the field of HIV/AIDS should be alert to and address ambivalence on use of medicinal plants. Existing programmes that enable women to learn about new information on HIV/AIDS should be strengthened.
- Full Text:
- Authors: Shonhai, Venencia F
- Date: 2009
- Subjects: Keiskamma Art Project (South Africa) , Women caregivers -- South Africa -- Eastern Cape , HIV infections -- Study and teaching -- South Africa , AIDS (Disease) -- Study and teaching -- South Africa , HIV-positive persons -- Care -- South Africa -- Eastern Cape , Medicinal plants -- South Africa , Natural foods -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1768 , http://hdl.handle.net/10962/d1003653
- Description: The scale of people being infected by HIV/AIDS (Human Immunodeficiency Virus /Acquired Immune Deficiency Syndrome) has meant that the family and the community have had to become involved in caring for the sick (Van Dyk, 2005). This has inevitably led to the emergence of informal caregivers in the form of family members caring for their relatives (Kipp, Nkosi, Laing & Jhangri, 2006). The research investigated the social learning of women caregivers looking after people living with HIV/AIDS, with emphasis on caregiving practices related to how they use traditional foods and medicinal plants. The research was undertaken in Grahamstown at the Raphael Centre and in Hamburg at Keiskkamma Health Centre and Art Project, Eastern Cape, South Africa. Data was collected using interviews, focus group discussions and diaries written by participants. The data was analyzed in two phases: the first phase involved reading the interview transcripts and collating the responses into analytical memos that were captured into broad categories, while the second phase made use of the community of practice analytical framework to further analyze the data to get better understanding of the social learning processes. This study reveals that participating in a community of practice like Raphael Centre and Keiskamma Health Centre enables caregivers to learn about caregiving. It also reveals that within these communities of practice there are varied learning processes that take place, such as observational and collaborative learning. The research also revealed that caregivers learn from the communities from which they come, for example caregivers learn about traditional food and medicinal plants which they use from their family members, friends, other caregivers as well as non governmental organizations. The research found that caregivers are influenced in their learning and practices by a number of factors which include their own experiences, ambivalent messages from different stakeholders concerned with fighting HIV/AIDS and exposure to new information. The research recommends that diverse learning processes in a community of practice and outside a community of practice should be encouraged and strengthened. It also recommends that HIV/AIDS caregiving options should be strengthened by drawing on experience and knowledge of caregivers. Caregivers should be encouraged to be selfsustaining to improve their caregiving practices. Stakeholders in the field of HIV/AIDS should be alert to and address ambivalence on use of medicinal plants. Existing programmes that enable women to learn about new information on HIV/AIDS should be strengthened.
- Full Text:
Student-athletes' attitude formation towards sport or other psychology services
- Wilmot, Gregory Thomas Charlton
- Authors: Wilmot, Gregory Thomas Charlton
- Date: 2009
- Subjects: Sports -- Psychological aspects Attitude (Psychology) Stereotypes (Social psychology) Clinical psychology Athletes -- Counseling of Athletes -- Attitudes
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3086 , http://hdl.handle.net/10962/d1002595
- Description: This qualitative study aims to explore attitude formation within a small sample of Tertiary level student-athletes in relation to their antecedent experiences, individual knowledge about psychology and the social factors impacting on their attitude formation. The study is intended to allow for the development of themes related to the participants’ personal responses to the questions posed to them. The participants’ answers from the interviews will allow for an in-depth analysis and interpretation of the constituents influencing attitude formation towards sport psychology consultation. Primarily, the study aims to explore how attitudes towards clinical and sport psychology practitioners are constructed in this sample. Clinical psychology is used as a contrasting reference point to explore attitude formation in sport psychology. The participants’ beliefs, knowledge sources and attitude toward clinical psychology are examined to understand the fundamental constituents of their attitudes and to understand how this might be related to attitude formation towards sport psychology. It is intended that exploration of the participants’ knowledge sources (e.g. parents, peers, media and coaches) and additional influences on attitudes towards clinical psychology are used to provide insight into how they might shape attitude formation toward sport psychology. This is viewed as particularly important as sport psychology is an increasingly important tool in assisting the enhancement of sporting performance output. This qualitative study uses four elite student-athletes from the same sporting discipline and contextual background to allow for close linkages between the participants’ responses about attitude formation to be made. The study further aims to explore and understand how similarities and differences occur between the participants’ responses which will be completed through analysing and drawing parallels within themes relating to the literature or emerging separately. The Literature Review is intended to help guide the research through providing a reference source for contextualizing individual, public and socially generated beliefs and knowledge (literacy) about mental health / psychology, the constituents of attitude formation and the student-athlete’s context. These factors were identified in the literature as crucial components in attitude formation and also assisted in the development of initial themes, thus guiding the research. Unfortunately, there was limited literature pertaining to attitude formation in relation to sport psychology services and thus provide justification for using clinical psychology to explore attitude formation in sport psychology. This initial exploration of attitude formation in clinical psychology was used as a ‘template’ for contrasting attitude formation in the sample student-athletes as an indication of attitude formation in sport psychology. The Literature Review is viewed as an integral and active component of the study that is used as a body of reference guiding the exploration and interpretation of the emergent themes. This is done through the iterative reference to past research relating to attitude formation, health beliefs, and the numerous sources of information influencing attitude formation within clinical psychology and the student-athlete context. Most importantly, the Literature Review provides a significant base of information that guides the Research Question and the particular factors that have been noted as the most significant to the research. The Literature Review is intended to guide the Research Question and highlight the significant components that need to be taken into consideration in exploring attitude formation. This study, having taken the numerous individual, social, contextual and other factors into consideration, seeks to understand how such factors combine to shape attitudes and influence attitude formation. The collection of literature on social, knowledge and antecedent factors are significant in their role of shaping attitude formation and thus have been included within the Research Question. Furthermore, the Research Question and Literature Review had an impact on the Methodology and why Thematic Analysis was selected. Given the similarities in the participants’ sporting and contextual backgrounds (e.g., being in the same crew, university and social context), it was felt that the primary determinants of attitude formation, within antecedent experiences, knowledge and social influences, would emerge in related themes and thus provide greater insight into attitude formation within the student-athlete context. A limiting factor however, does lie in the small number of participants from a limited diversity of backgrounds, experiences or factors that could assist in allowing broader generalisations of the findings. However, this study aims to understand how the particular factors influence or shape attitudes towards sport and other psychology services and aims to understand the more specific or ‘fundamental’ components of attitude formation that are experienced within the student-athlete’s context and environment.
- Full Text:
- Authors: Wilmot, Gregory Thomas Charlton
- Date: 2009
- Subjects: Sports -- Psychological aspects Attitude (Psychology) Stereotypes (Social psychology) Clinical psychology Athletes -- Counseling of Athletes -- Attitudes
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3086 , http://hdl.handle.net/10962/d1002595
- Description: This qualitative study aims to explore attitude formation within a small sample of Tertiary level student-athletes in relation to their antecedent experiences, individual knowledge about psychology and the social factors impacting on their attitude formation. The study is intended to allow for the development of themes related to the participants’ personal responses to the questions posed to them. The participants’ answers from the interviews will allow for an in-depth analysis and interpretation of the constituents influencing attitude formation towards sport psychology consultation. Primarily, the study aims to explore how attitudes towards clinical and sport psychology practitioners are constructed in this sample. Clinical psychology is used as a contrasting reference point to explore attitude formation in sport psychology. The participants’ beliefs, knowledge sources and attitude toward clinical psychology are examined to understand the fundamental constituents of their attitudes and to understand how this might be related to attitude formation towards sport psychology. It is intended that exploration of the participants’ knowledge sources (e.g. parents, peers, media and coaches) and additional influences on attitudes towards clinical psychology are used to provide insight into how they might shape attitude formation toward sport psychology. This is viewed as particularly important as sport psychology is an increasingly important tool in assisting the enhancement of sporting performance output. This qualitative study uses four elite student-athletes from the same sporting discipline and contextual background to allow for close linkages between the participants’ responses about attitude formation to be made. The study further aims to explore and understand how similarities and differences occur between the participants’ responses which will be completed through analysing and drawing parallels within themes relating to the literature or emerging separately. The Literature Review is intended to help guide the research through providing a reference source for contextualizing individual, public and socially generated beliefs and knowledge (literacy) about mental health / psychology, the constituents of attitude formation and the student-athlete’s context. These factors were identified in the literature as crucial components in attitude formation and also assisted in the development of initial themes, thus guiding the research. Unfortunately, there was limited literature pertaining to attitude formation in relation to sport psychology services and thus provide justification for using clinical psychology to explore attitude formation in sport psychology. This initial exploration of attitude formation in clinical psychology was used as a ‘template’ for contrasting attitude formation in the sample student-athletes as an indication of attitude formation in sport psychology. The Literature Review is viewed as an integral and active component of the study that is used as a body of reference guiding the exploration and interpretation of the emergent themes. This is done through the iterative reference to past research relating to attitude formation, health beliefs, and the numerous sources of information influencing attitude formation within clinical psychology and the student-athlete context. Most importantly, the Literature Review provides a significant base of information that guides the Research Question and the particular factors that have been noted as the most significant to the research. The Literature Review is intended to guide the Research Question and highlight the significant components that need to be taken into consideration in exploring attitude formation. This study, having taken the numerous individual, social, contextual and other factors into consideration, seeks to understand how such factors combine to shape attitudes and influence attitude formation. The collection of literature on social, knowledge and antecedent factors are significant in their role of shaping attitude formation and thus have been included within the Research Question. Furthermore, the Research Question and Literature Review had an impact on the Methodology and why Thematic Analysis was selected. Given the similarities in the participants’ sporting and contextual backgrounds (e.g., being in the same crew, university and social context), it was felt that the primary determinants of attitude formation, within antecedent experiences, knowledge and social influences, would emerge in related themes and thus provide greater insight into attitude formation within the student-athlete context. A limiting factor however, does lie in the small number of participants from a limited diversity of backgrounds, experiences or factors that could assist in allowing broader generalisations of the findings. However, this study aims to understand how the particular factors influence or shape attitudes towards sport and other psychology services and aims to understand the more specific or ‘fundamental’ components of attitude formation that are experienced within the student-athlete’s context and environment.
- Full Text:
Studies towards the synthesis of novel tridentate ligands for use in ruthenium metathesis catalysts
- Authors: Millward, Tanya
- Date: 2009
- Subjects: Ligands , Catalysis , Metathesis (Chemistry) , Ruthenium , Complex compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4360 , http://hdl.handle.net/10962/d1005025 , Ligands , Catalysis , Metathesis (Chemistry) , Ruthenium , Complex compounds
- Description: This work has focussed on the preparation of a variety of tridentate ligands, designed to form ruthenium complexes as potential metathesis catalysts. Various approaches to the tridentate, malonate-tethered imidazolidine system have been investigated, and a promising route to accessing ligands of this type is discussed. A tridentate malonate-tethered pyridine ligand has been successfully prepared and its dithallium salt has been accessed by hydrolysis with thallium carbonate; approaches to a longer-chain analogue have also been investigated. A thallium pyridine-2,6- dicarboxylate ligand has been has been successfully prepared, as have a range of pyridine diamine ligands, with various alkyl and aromatic substituents on the amine donor atoms. Preliminary investigations into the potential of these compounds as ligands for alkylidene ruthenium complexes are reported using molecular modelling techniques. The geometries and steric energies of the ligands and their corresponding complexes have been analysed, and results obtained from two different software packages are compared. Finally, some preliminary complexation studies have been undertaken.
- Full Text:
- Authors: Millward, Tanya
- Date: 2009
- Subjects: Ligands , Catalysis , Metathesis (Chemistry) , Ruthenium , Complex compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4360 , http://hdl.handle.net/10962/d1005025 , Ligands , Catalysis , Metathesis (Chemistry) , Ruthenium , Complex compounds
- Description: This work has focussed on the preparation of a variety of tridentate ligands, designed to form ruthenium complexes as potential metathesis catalysts. Various approaches to the tridentate, malonate-tethered imidazolidine system have been investigated, and a promising route to accessing ligands of this type is discussed. A tridentate malonate-tethered pyridine ligand has been successfully prepared and its dithallium salt has been accessed by hydrolysis with thallium carbonate; approaches to a longer-chain analogue have also been investigated. A thallium pyridine-2,6- dicarboxylate ligand has been has been successfully prepared, as have a range of pyridine diamine ligands, with various alkyl and aromatic substituents on the amine donor atoms. Preliminary investigations into the potential of these compounds as ligands for alkylidene ruthenium complexes are reported using molecular modelling techniques. The geometries and steric energies of the ligands and their corresponding complexes have been analysed, and results obtained from two different software packages are compared. Finally, some preliminary complexation studies have been undertaken.
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