Establishing experimental systems for studying the replication biology of Providence virus
- Authors: Walter, Cheryl Tracy
- Date: 2009
- Subjects: Insects -- Viruses Insects -- Diseases Insects -- Parasites Host-virus relationships RNA viruses DNA Insects as carriers of disease
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3928 , http://hdl.handle.net/10962/d1003987
- Description: Providence virus (PrV) is a member of the Tetraviridae, a family of small, positive sense, single-stranded RNA viruses, which characteristically infect the midgut tissue of heliothine larvae. PrV is the only known tetravirus that replicates in cultured insect cells. The virus comprises a monopartite genome resembling members of the genus Betatetravirus with the capsid precursor protein undergoing autoproteolytic cleavage at its C-terminus consistent with other tetravirus capsid precursor proteins. Analysis of viral cDNA predicted the presence of three potential overlapping gene products (from 5` to 3`): (1) p130, a protein of unrecognized nucleotide or amino acid homology with a 2A-like processing site at its N-terminus; (2) p104, the replicase ORF, which was found to be phylogenetically related to tombus-and umbraviruses replicases. The presence of a read-through stop signal in the p104 ORF was proposed to produce and amino terminal product with a predicted MW of 40 kDa (p40) and (3) the capsid protein precursor (81 kDa) which has two 2A-like processing sites at its N-terminus. Metabolic radiolabelling of viral translation products in persistently infected MG8 cells and in vitro translation of the individual ORFs were performed in order to analyse the expression of PrV gene products. p130 was translated with no evidence of 2A-like processing. Two products of 40 kDa and 104 kDa were translated from the p104 ORF, indicating that the read-through stop signal was likely to be functional. Finally, the capsid protein precursor ORF produced a major translation product of 68 kDa corresponding to the capsid protein precursor as well a peptide of 15 kDa that was attributed to the activity of the second 2A-like site at the N-terminus of the p81 ORF. The subcellular distribution of viral RNA (vRNA) and p40 in MG8 cells was investigated using immunofluorescence and biochemical fractionation. The results showed that p40/p104 and vRNA accumulated in polarized, punctate structures in some but not all MG8 cells and in some cases, co-localization was observed. This thesis concludes that PrV is a novel tetravirus with significant similarities plant carmolike viruses that should be re-classified at the family level.
- Full Text:
- Date Issued: 2009
- Authors: Walter, Cheryl Tracy
- Date: 2009
- Subjects: Insects -- Viruses Insects -- Diseases Insects -- Parasites Host-virus relationships RNA viruses DNA Insects as carriers of disease
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3928 , http://hdl.handle.net/10962/d1003987
- Description: Providence virus (PrV) is a member of the Tetraviridae, a family of small, positive sense, single-stranded RNA viruses, which characteristically infect the midgut tissue of heliothine larvae. PrV is the only known tetravirus that replicates in cultured insect cells. The virus comprises a monopartite genome resembling members of the genus Betatetravirus with the capsid precursor protein undergoing autoproteolytic cleavage at its C-terminus consistent with other tetravirus capsid precursor proteins. Analysis of viral cDNA predicted the presence of three potential overlapping gene products (from 5` to 3`): (1) p130, a protein of unrecognized nucleotide or amino acid homology with a 2A-like processing site at its N-terminus; (2) p104, the replicase ORF, which was found to be phylogenetically related to tombus-and umbraviruses replicases. The presence of a read-through stop signal in the p104 ORF was proposed to produce and amino terminal product with a predicted MW of 40 kDa (p40) and (3) the capsid protein precursor (81 kDa) which has two 2A-like processing sites at its N-terminus. Metabolic radiolabelling of viral translation products in persistently infected MG8 cells and in vitro translation of the individual ORFs were performed in order to analyse the expression of PrV gene products. p130 was translated with no evidence of 2A-like processing. Two products of 40 kDa and 104 kDa were translated from the p104 ORF, indicating that the read-through stop signal was likely to be functional. Finally, the capsid protein precursor ORF produced a major translation product of 68 kDa corresponding to the capsid protein precursor as well a peptide of 15 kDa that was attributed to the activity of the second 2A-like site at the N-terminus of the p81 ORF. The subcellular distribution of viral RNA (vRNA) and p40 in MG8 cells was investigated using immunofluorescence and biochemical fractionation. The results showed that p40/p104 and vRNA accumulated in polarized, punctate structures in some but not all MG8 cells and in some cases, co-localization was observed. This thesis concludes that PrV is a novel tetravirus with significant similarities plant carmolike viruses that should be re-classified at the family level.
- Full Text:
- Date Issued: 2009
Ethical decision-making in the therapeutic space : a psychoanalytic view
- Authors: Silove, Melanie
- Date: 2009
- Subjects: Decision making -- Moral and ethical aspects , Psychotherapy -- Moral and ethical aspects , Psychotherapists -- Professional ethics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3273 , http://hdl.handle.net/10962/d1020873
- Description: This study examined the ethical decision-making process as it transpired in the everyday context of the therapeutic space. In-depth interviews explored the subjective experiences of six South African psychologists, practicing as psychoanalytic psychotherapists, and their efforts to resolve real-life ethical dilemmas. The theoretical framework used to interpret the data subsumed professional literature in psychology on principle-based ethical decision-making as well as contemporary psychoanalytic debates on the phenomenon of countertransference enactments. A review of ethics codes, survey research and seminal decision-making frameworks suggests that ethical dilemmas have traditionally been resolved by recourse to an objective and impartial “principle ethics” perspective. Empirical evidence shows, however, that logical thinking and the rational application of codes, principles and standards are often insufficient to secure ethical action. The establishment of reflective space and the core theoretical notion of “ethical decision-making enactments” were proposed in order to address the subjective, irrational and unconscious dimension of professional decision-making. This study used a broadly hermeneutic research method which transformed participants‟ descriptions of engagement with real-life dilemmas into a psychoanalytically informed interpretive account of ethical decision-making. Twelve aspirational ethical principles were found to guide participants‟ daily analytic work. Beneficence was the principle most strongly identified with and nonmaleficence was the most neglected ethical principle. Unprocessed countertransference responses were shown to drive earlier prereflective phases of the ethical decision-making process. Mature ethical judgment was predicated upon the retrospective analysis of enactment phenomena. Dissatisfaction was expressed by all participants with regard to the role of professional resources in aiding the resolution of stressful ethical dilemmas. Risk factors for compromised professional decision-making included the paucity and perceived irrelevance of postgraduate ethics training, supervisory failure to confront the ethical and countertransference dimensions of common dilemmas and professional isolation. Rather than eliciting the hope of emotional support and greater insight, professional resources on the contrary mostly appeared to induce anxiety, mistrust and fearfulness. Based on the data and the literature, a pragmatic psychoanalytically informed ethical decision-making model was finally generated. The model, which considers both principle ethics as well as countertransference phenomena, offers a preliminary contribution to professional dialogue on the development and evaluation of empirically based decision-making frameworks. Practical recommendations are made for both the revision of the current South African ethics code and for improving the postqualifying ethics education of psychoanalytic practitioners and supervisors. The limitations of the data are discussed and directions for future research initiatives are proposed.
- Full Text:
- Date Issued: 2009
- Authors: Silove, Melanie
- Date: 2009
- Subjects: Decision making -- Moral and ethical aspects , Psychotherapy -- Moral and ethical aspects , Psychotherapists -- Professional ethics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3273 , http://hdl.handle.net/10962/d1020873
- Description: This study examined the ethical decision-making process as it transpired in the everyday context of the therapeutic space. In-depth interviews explored the subjective experiences of six South African psychologists, practicing as psychoanalytic psychotherapists, and their efforts to resolve real-life ethical dilemmas. The theoretical framework used to interpret the data subsumed professional literature in psychology on principle-based ethical decision-making as well as contemporary psychoanalytic debates on the phenomenon of countertransference enactments. A review of ethics codes, survey research and seminal decision-making frameworks suggests that ethical dilemmas have traditionally been resolved by recourse to an objective and impartial “principle ethics” perspective. Empirical evidence shows, however, that logical thinking and the rational application of codes, principles and standards are often insufficient to secure ethical action. The establishment of reflective space and the core theoretical notion of “ethical decision-making enactments” were proposed in order to address the subjective, irrational and unconscious dimension of professional decision-making. This study used a broadly hermeneutic research method which transformed participants‟ descriptions of engagement with real-life dilemmas into a psychoanalytically informed interpretive account of ethical decision-making. Twelve aspirational ethical principles were found to guide participants‟ daily analytic work. Beneficence was the principle most strongly identified with and nonmaleficence was the most neglected ethical principle. Unprocessed countertransference responses were shown to drive earlier prereflective phases of the ethical decision-making process. Mature ethical judgment was predicated upon the retrospective analysis of enactment phenomena. Dissatisfaction was expressed by all participants with regard to the role of professional resources in aiding the resolution of stressful ethical dilemmas. Risk factors for compromised professional decision-making included the paucity and perceived irrelevance of postgraduate ethics training, supervisory failure to confront the ethical and countertransference dimensions of common dilemmas and professional isolation. Rather than eliciting the hope of emotional support and greater insight, professional resources on the contrary mostly appeared to induce anxiety, mistrust and fearfulness. Based on the data and the literature, a pragmatic psychoanalytically informed ethical decision-making model was finally generated. The model, which considers both principle ethics as well as countertransference phenomena, offers a preliminary contribution to professional dialogue on the development and evaluation of empirically based decision-making frameworks. Practical recommendations are made for both the revision of the current South African ethics code and for improving the postqualifying ethics education of psychoanalytic practitioners and supervisors. The limitations of the data are discussed and directions for future research initiatives are proposed.
- Full Text:
- Date Issued: 2009
Evaluating uncertainty in water resources estimation in Southern Africa : a case study of South Africa
- Authors: Sawunyama, Tendai
- Date: 2009
- Subjects: Water supply -- South Africa , Water supply -- Africa, Southern , Hydrology -- South Africa , Hydrology -- Africa, Southern , Hydrologic models , Hydrology research -- South Africa , Hydrology research -- Africa, Southern , Rain and rainfall -- Mathematical models , Runoff -- Mathematical models
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6035 , http://hdl.handle.net/10962/d1006176
- Description: Hydrological models are widely used tools in water resources estimation, but they are simple representations of reality and are frequently based on inadequate input data and uncertainties in parameter values. Data observation networks are expensive to establish and maintain and often beyond the resources of most developing countries. Consequently, measurements are difficult to obtain and observation networks in many countries are shrinking, hence obtaining representative observations in space and time remains a challenge. This study presents some guidelines on the identification, quantification and reduction of sources of uncertainty in water resources estimation in southern Africa, a data scarce region. The analyses are based on example sub-basins drawn from South Africa and the application of the Pitman hydrological model. While it has always been recognised that estimates of water resources availability for the region are subject to possible errors, the quantification of these uncertainties has never been explicitly incorporated into the methods used in the region. The motivation for this study was therefore to contribute to the future development of a revised framework for water resources estimation that does include uncertainty. The focus was on uncertainties associated with climate input data, parameter estimation (and recognizing the uncertainty due model structure deficiencies) methods and water use data. In addition to variance based measures of uncertainty, this study also used a reservoir yield based statistic to evaluate model output uncertainty, which represents an integrated measure of flow regime variations and one that can be more easily understood by water resources managers. Through a sensitivity analysis approach, the results of the individual contribution of each source of uncertainty suggest regional differences and that clear statements about which source of uncertainty is likely to dominate are not generally possible. Parameter sensitivity analysis was used in identifying parameters which are important withinspecific sub-basins and therefore those to focus on in uncertainty analysis. The study used a simple framework for evaluating the combined contribution of uncertainty sources to model outputs that is consistent with the model limitations and data available, and that allows direct quantitative comparison between model outputs obtained by using different sources of information and methods within Spatial and Time Series Information Modelling (SPATSIM) software. The results from combining the sources of uncertainties showed that parameter uncertainty dominates the contribution to model output uncertainty. However, in some parts of the country especially those with complex topography, which tend to experience high rainfall spatial variability, rainfall uncertainty is equally dominant, while the contributions of evaporation and water use data uncertainty are relatively small. While the results of this study are encouraging, the weaknesses of the methods used to quantify uncertainty (especially subjectivity involved in evaluating parameter uncertainty) should not be neglected and require further evaluations. An effort to reduce data and parameter uncertainty shows that this can only be achieved if data access at appropriate scale and quality improves. Perhaps the focus should be on maintaining existing networks and concentrating research efforts on making the most out of the emerging data products derived from remote sensing platforms. While this study presents some initial guidelines for evaluating uncertainty in South Africa, there is need to overcome several constraints which are related to data availability and accuracy, the models used and the capacity or willingness to adopt new methods that incorporate uncertainty. The study has provided a starting point for the development of new approaches to modelling water resources in the region that include uncertain estimates.
- Full Text:
- Date Issued: 2009
- Authors: Sawunyama, Tendai
- Date: 2009
- Subjects: Water supply -- South Africa , Water supply -- Africa, Southern , Hydrology -- South Africa , Hydrology -- Africa, Southern , Hydrologic models , Hydrology research -- South Africa , Hydrology research -- Africa, Southern , Rain and rainfall -- Mathematical models , Runoff -- Mathematical models
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6035 , http://hdl.handle.net/10962/d1006176
- Description: Hydrological models are widely used tools in water resources estimation, but they are simple representations of reality and are frequently based on inadequate input data and uncertainties in parameter values. Data observation networks are expensive to establish and maintain and often beyond the resources of most developing countries. Consequently, measurements are difficult to obtain and observation networks in many countries are shrinking, hence obtaining representative observations in space and time remains a challenge. This study presents some guidelines on the identification, quantification and reduction of sources of uncertainty in water resources estimation in southern Africa, a data scarce region. The analyses are based on example sub-basins drawn from South Africa and the application of the Pitman hydrological model. While it has always been recognised that estimates of water resources availability for the region are subject to possible errors, the quantification of these uncertainties has never been explicitly incorporated into the methods used in the region. The motivation for this study was therefore to contribute to the future development of a revised framework for water resources estimation that does include uncertainty. The focus was on uncertainties associated with climate input data, parameter estimation (and recognizing the uncertainty due model structure deficiencies) methods and water use data. In addition to variance based measures of uncertainty, this study also used a reservoir yield based statistic to evaluate model output uncertainty, which represents an integrated measure of flow regime variations and one that can be more easily understood by water resources managers. Through a sensitivity analysis approach, the results of the individual contribution of each source of uncertainty suggest regional differences and that clear statements about which source of uncertainty is likely to dominate are not generally possible. Parameter sensitivity analysis was used in identifying parameters which are important withinspecific sub-basins and therefore those to focus on in uncertainty analysis. The study used a simple framework for evaluating the combined contribution of uncertainty sources to model outputs that is consistent with the model limitations and data available, and that allows direct quantitative comparison between model outputs obtained by using different sources of information and methods within Spatial and Time Series Information Modelling (SPATSIM) software. The results from combining the sources of uncertainties showed that parameter uncertainty dominates the contribution to model output uncertainty. However, in some parts of the country especially those with complex topography, which tend to experience high rainfall spatial variability, rainfall uncertainty is equally dominant, while the contributions of evaporation and water use data uncertainty are relatively small. While the results of this study are encouraging, the weaknesses of the methods used to quantify uncertainty (especially subjectivity involved in evaluating parameter uncertainty) should not be neglected and require further evaluations. An effort to reduce data and parameter uncertainty shows that this can only be achieved if data access at appropriate scale and quality improves. Perhaps the focus should be on maintaining existing networks and concentrating research efforts on making the most out of the emerging data products derived from remote sensing platforms. While this study presents some initial guidelines for evaluating uncertainty in South Africa, there is need to overcome several constraints which are related to data availability and accuracy, the models used and the capacity or willingness to adopt new methods that incorporate uncertainty. The study has provided a starting point for the development of new approaches to modelling water resources in the region that include uncertain estimates.
- Full Text:
- Date Issued: 2009
Evaluation of a plant-herbivore system in determining potential efficacy of a candidate biological control agent, cornops aquaticum for water hyacinth, eichhornia crassipes
- Authors: Bownes, Angela
- Date: 2009
- Subjects: Water hyacinth -- Control -- South Africa , Eichhornia crassipedes , Pontederiaceae , Grasshoppers , Biological pest control agents -- South Africa , Weeds -- Biological control -- South Africa , Invasive plants -- Biological control -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5687 , http://hdl.handle.net/10962/d1005373
- Description: Water hyacinth, Eichhornia crassipes Mart. Solms-Laubach (Pontederiaceae), a freefloating aquatic macrophyte of Neotropical origin, was introduced into South Africa as an ornamental aquarium plant in the early 1900’s. By the 1970’s it had reached pest proportions in dams and rivers around the country. Due to the sustainability, cost efficiency and low environmental risk associated with biological control, this has been a widely used method in an attempt to reduce infestations to below the threshold where they cause economic and ecological damage. To date, five arthropod and one pathogen biocontrol agents have been introduced for the control of water hyacinth but their impact has been variable. It is believed that their efficacy is hampered by the presence of highly eutrophic systems in South Africa in which plant growth is prolific and the negative effects of herbivory are therefore mitigated. It is for these reasons that new, potentially more damaging biocontrol agents are being considered for release. The water hyacinth grasshopper, Cornops aquaticum Brüner (Orthoptera: Acrididae), which is native to South America and Mexico, was brought into quarantine in Pretoria, South Africa in 1995. Although the grasshopper was identified as one of the most damaging insects associated with water hyacinth in its native range, it has not been considered as a biocontrol agent for water hyacinth anywhere else in the world. After extensive host-range testing which revealed it to be safe for release, a release permit for this candidate agent was issued in 2007. However, host specificity testing is no longer considered to be the only important component of pre-release screening of candidate biocontrol agents. Investigating biological and ecological aspects of the plant-herbivore system that will assist in determination of potential establishment, efficacy and the ability to build up good populations in the recipient environment are some of the important factors. This thesis is a pre-release evaluation of C. aquaticum to determine whether it is sufficiently damaging to water hyacinth to warrant its release. It investigated interactions between the grasshopper and water hyacinth under a range of nutrient conditions found in South African water bodies as well as the impact of the grasshopper on the competitive performance of water hyacinth. Both plant growth rates and the response of water hyacinth to herbivory by the grasshopper were influenced by nutrient availability to the plants. The ability of water hyacinth to compensate for loss of tissue through herbivory was greater under eutrophic nutrient conditions. However, a negative linear relationship was found between grasshopper biomass and water hyacinth performance parameters such as biomass accumulation and leaf production, even under eutrophic conditions. Water hyacinth’s compensatory ability in terms of its potential to mitigate to detrimental effects of insect feeding was dependent on the amount of damage caused by herbivory by the grasshopper. Plant biomass and the competitive ability of water hyacinth in relation to another freefloating aquatic weed species were reduced by C. aquaticum under eutrophic nutrient conditions, in a short space of time. It was also found that grasshopper feeding and characteristics related to their population dynamics such as fecundity and survival were significantly influenced by water nutrient availability and that environmental nutrient availability will influence the control potential of this species should it be released in South Africa. Cornops aquaticum shows promise as a biocontrol agent for water hyacinth but additional factors that were not investigated in this study such as compatibility with the South African climate and the current water hyacinth biocontrol agents need to be combined with these data to make a decision on its release. Possible management options for this species if it is to be introduced into South Africa are discussed.
- Full Text:
- Date Issued: 2009
- Authors: Bownes, Angela
- Date: 2009
- Subjects: Water hyacinth -- Control -- South Africa , Eichhornia crassipedes , Pontederiaceae , Grasshoppers , Biological pest control agents -- South Africa , Weeds -- Biological control -- South Africa , Invasive plants -- Biological control -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5687 , http://hdl.handle.net/10962/d1005373
- Description: Water hyacinth, Eichhornia crassipes Mart. Solms-Laubach (Pontederiaceae), a freefloating aquatic macrophyte of Neotropical origin, was introduced into South Africa as an ornamental aquarium plant in the early 1900’s. By the 1970’s it had reached pest proportions in dams and rivers around the country. Due to the sustainability, cost efficiency and low environmental risk associated with biological control, this has been a widely used method in an attempt to reduce infestations to below the threshold where they cause economic and ecological damage. To date, five arthropod and one pathogen biocontrol agents have been introduced for the control of water hyacinth but their impact has been variable. It is believed that their efficacy is hampered by the presence of highly eutrophic systems in South Africa in which plant growth is prolific and the negative effects of herbivory are therefore mitigated. It is for these reasons that new, potentially more damaging biocontrol agents are being considered for release. The water hyacinth grasshopper, Cornops aquaticum Brüner (Orthoptera: Acrididae), which is native to South America and Mexico, was brought into quarantine in Pretoria, South Africa in 1995. Although the grasshopper was identified as one of the most damaging insects associated with water hyacinth in its native range, it has not been considered as a biocontrol agent for water hyacinth anywhere else in the world. After extensive host-range testing which revealed it to be safe for release, a release permit for this candidate agent was issued in 2007. However, host specificity testing is no longer considered to be the only important component of pre-release screening of candidate biocontrol agents. Investigating biological and ecological aspects of the plant-herbivore system that will assist in determination of potential establishment, efficacy and the ability to build up good populations in the recipient environment are some of the important factors. This thesis is a pre-release evaluation of C. aquaticum to determine whether it is sufficiently damaging to water hyacinth to warrant its release. It investigated interactions between the grasshopper and water hyacinth under a range of nutrient conditions found in South African water bodies as well as the impact of the grasshopper on the competitive performance of water hyacinth. Both plant growth rates and the response of water hyacinth to herbivory by the grasshopper were influenced by nutrient availability to the plants. The ability of water hyacinth to compensate for loss of tissue through herbivory was greater under eutrophic nutrient conditions. However, a negative linear relationship was found between grasshopper biomass and water hyacinth performance parameters such as biomass accumulation and leaf production, even under eutrophic conditions. Water hyacinth’s compensatory ability in terms of its potential to mitigate to detrimental effects of insect feeding was dependent on the amount of damage caused by herbivory by the grasshopper. Plant biomass and the competitive ability of water hyacinth in relation to another freefloating aquatic weed species were reduced by C. aquaticum under eutrophic nutrient conditions, in a short space of time. It was also found that grasshopper feeding and characteristics related to their population dynamics such as fecundity and survival were significantly influenced by water nutrient availability and that environmental nutrient availability will influence the control potential of this species should it be released in South Africa. Cornops aquaticum shows promise as a biocontrol agent for water hyacinth but additional factors that were not investigated in this study such as compatibility with the South African climate and the current water hyacinth biocontrol agents need to be combined with these data to make a decision on its release. Possible management options for this species if it is to be introduced into South Africa are discussed.
- Full Text:
- Date Issued: 2009
Hierarchical spatial structure and levels of resolution of intertidal grazing and their consequences on predictability and stability at small scales
- Authors: Diaz Diaz, Eliecer Rodrigo
- Date: 2009
- Subjects: Marine algae -- Effect of grazing on Shorelines -- South Africa Zostera marina Intertidal ecology Spatial analysis (Statistics)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5707 , http://hdl.handle.net/10962/d1005393
- Description: The aim of this research was to assess three hierarchical aspects of alga-grazer interactions in intertidal communities on a small scale: spatial heterogeneity, grazing effects and spatial stability in grazing effects. First, using semivariograms and cross-semivariograms I observed hierarchical spatial patterns in most algal groups and in grazers. However, these patterns varied with the level on the shore and between shores, suggesting that either human exploitation or wave exposure can be a source of variability. Second, grazing effects were studied using manipulative experiments at different levels on the shore. These revealed significant effects of grazing on the low shore and in tidal pools. Additionally, using a transect of grazer exclusions across the shore, I observed unexpected hierarchical patchiness in the strength of grazing, rather than zonation in its effects. This patchiness varied in time due to different biotic and abiotic factors. In a separate experiment, the effect of mesograzers effects were studied in the upper eulittoral zone under four conditions: burnt open rock (BOR), burnt pools (Bpool), non-burnt open rock (NBOR) and non-burnt pools (NBpool). Additionally, I tested spatial stability in the effects of grazing in consecutive years, using the same plots. I observed great spatial variability in the effects of grazing, but this variability was spatially stable in Bpools and NBOR, meaning deterministic and significant grazing effects in consecutive years on the same plots. Both the significance in grazing effects and spatial stability depended on the level of resolution (species, functional, biomass) at which the algal assemblage was evaluated, suggesting hierarchical variability. In order to be able to predict spatial variability in the effects of grazers in the upper eulittoral zone using biotic and abiotic micro- and macrofactors, a conceptual model was proposed, based on data from several multiple-regressions. This linked the interactions among three elements: idiosyncratic heterogeneity, micro and macrofactors. This suggests that spatial variability can be a product of these factors, while spatial stability can be caused by the same or different combinations of factors. In conclusion, grazing and other ecological phenomena must be studied hierarchically, not only through spatiotemporal scales, but also at different levels of resolution, as these also influence our perception of patterns.
- Full Text:
- Date Issued: 2009
- Authors: Diaz Diaz, Eliecer Rodrigo
- Date: 2009
- Subjects: Marine algae -- Effect of grazing on Shorelines -- South Africa Zostera marina Intertidal ecology Spatial analysis (Statistics)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5707 , http://hdl.handle.net/10962/d1005393
- Description: The aim of this research was to assess three hierarchical aspects of alga-grazer interactions in intertidal communities on a small scale: spatial heterogeneity, grazing effects and spatial stability in grazing effects. First, using semivariograms and cross-semivariograms I observed hierarchical spatial patterns in most algal groups and in grazers. However, these patterns varied with the level on the shore and between shores, suggesting that either human exploitation or wave exposure can be a source of variability. Second, grazing effects were studied using manipulative experiments at different levels on the shore. These revealed significant effects of grazing on the low shore and in tidal pools. Additionally, using a transect of grazer exclusions across the shore, I observed unexpected hierarchical patchiness in the strength of grazing, rather than zonation in its effects. This patchiness varied in time due to different biotic and abiotic factors. In a separate experiment, the effect of mesograzers effects were studied in the upper eulittoral zone under four conditions: burnt open rock (BOR), burnt pools (Bpool), non-burnt open rock (NBOR) and non-burnt pools (NBpool). Additionally, I tested spatial stability in the effects of grazing in consecutive years, using the same plots. I observed great spatial variability in the effects of grazing, but this variability was spatially stable in Bpools and NBOR, meaning deterministic and significant grazing effects in consecutive years on the same plots. Both the significance in grazing effects and spatial stability depended on the level of resolution (species, functional, biomass) at which the algal assemblage was evaluated, suggesting hierarchical variability. In order to be able to predict spatial variability in the effects of grazers in the upper eulittoral zone using biotic and abiotic micro- and macrofactors, a conceptual model was proposed, based on data from several multiple-regressions. This linked the interactions among three elements: idiosyncratic heterogeneity, micro and macrofactors. This suggests that spatial variability can be a product of these factors, while spatial stability can be caused by the same or different combinations of factors. In conclusion, grazing and other ecological phenomena must be studied hierarchically, not only through spatiotemporal scales, but also at different levels of resolution, as these also influence our perception of patterns.
- Full Text:
- Date Issued: 2009
Indigenous knowledges: a genealogy of representations and applications in developing contexts of environmental education and development in southern Africa
- Authors: Shava, Soul
- Date: 2009
- Subjects: Foucault, Michel, 1926-1984 Education -- Africa, Southern Environmental education -- Africa, Southern Indigenous peoples -- Africa, Southern Ethnoscience -- Africa, Southern Knowledge, Theory of Genealogy (Philosophy) Medicinal plants -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1885 , http://hdl.handle.net/10962/d1005920
- Description: This study was developed around concerns about how indigenous knowledges have been represented and applied in environment and development education. The first phase of the study is a genealogical analysis after Michel Foucault. This probes representations and applications of plant-based indigenous knowledge in selected anthropological, botanical and environmental education texts in southern Africa. The emerging insights were deepened using a Social (Critical) Realism vantage point after Margaret Archer to shed light on agential issues in environmental education and development contexts. Here her morphogenetic/morphostatic analysis of social transformation or reproduction is used to trace changes in indigenous knowledge representations and applications over time (from the pre-colonial into the post-colonial era). The second phase uses the same perspectives and tools to extend the analysis of power/knowledge relationships into the interface of indigenous communities and modern institutions in two case study settings in the Eastern Cape of South Africa. This study reveals colonially-derived hegemonic processes of modern/Western scientific institutional representations/interpretations of the knowledges of indigenous communities. It also tracks a continuing trajectory of their dominating and prescriptive mediating control over local knowledges from the pre-colonial context through into the post-colonial period in southern Africa. The analysis reveals how this hegemony is sustained through the deployment of institutional strategies of representation that transform local knowledges into the disciplinary knowledge discourses of modern scientific institutions. These representational strategies therefore generate/reproduce and validate disciplinary discourses about the other, constructing disciplinary 'regimes of truth'. In this way modern institutions appropriate and displace indigenous/local knowledges, silence the voices of local communities and regulate individual and community agency within a continuing subjugation of indigenous knowledges. This study reveals how working within modern institutions and disciplinary knowledges in participative education and development interactions can serve to implicate indigenous researchers in these institutional hegemonic processes. The study also notes evidence of a continued resistance to hegemonic Western knowledge discourses as indigenous communities have sustained many knowledge practices alongside Western knowledge discourses. There is also evidence of a recent emergence of counter-hegemonic indigenous knowledge discourses in environmental education and development practices in southern Africa. It is noted that these have been contingent upon the changing political terrain in southern Africa as this has opened the way for alternative discourses to the dominant conventional Western knowledges in formal education and development contexts. The counterhegemonic discourses invert power/knowledge relations, decentre hegemonic discourses and reposition indigenous knowledges in formal education and development contexts. This study suggests the need to foreground indigenous knowledges as a process of knowledge decolonisation that gives contextual and epistemic relevance to environmental education and development processes. This calls for a need for new strategies to transform existing institutions by creating enabling spaces for the representational inclusion of indigenous knowledges in formal/conventional knowledge discourses and their application in social contexts. This opens up possibilities for plural knowledge representations and for their integrative and reciprocal co-engagement in situated contexts of environmental education and development in southern Africa.
- Full Text:
- Date Issued: 2009
- Authors: Shava, Soul
- Date: 2009
- Subjects: Foucault, Michel, 1926-1984 Education -- Africa, Southern Environmental education -- Africa, Southern Indigenous peoples -- Africa, Southern Ethnoscience -- Africa, Southern Knowledge, Theory of Genealogy (Philosophy) Medicinal plants -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1885 , http://hdl.handle.net/10962/d1005920
- Description: This study was developed around concerns about how indigenous knowledges have been represented and applied in environment and development education. The first phase of the study is a genealogical analysis after Michel Foucault. This probes representations and applications of plant-based indigenous knowledge in selected anthropological, botanical and environmental education texts in southern Africa. The emerging insights were deepened using a Social (Critical) Realism vantage point after Margaret Archer to shed light on agential issues in environmental education and development contexts. Here her morphogenetic/morphostatic analysis of social transformation or reproduction is used to trace changes in indigenous knowledge representations and applications over time (from the pre-colonial into the post-colonial era). The second phase uses the same perspectives and tools to extend the analysis of power/knowledge relationships into the interface of indigenous communities and modern institutions in two case study settings in the Eastern Cape of South Africa. This study reveals colonially-derived hegemonic processes of modern/Western scientific institutional representations/interpretations of the knowledges of indigenous communities. It also tracks a continuing trajectory of their dominating and prescriptive mediating control over local knowledges from the pre-colonial context through into the post-colonial period in southern Africa. The analysis reveals how this hegemony is sustained through the deployment of institutional strategies of representation that transform local knowledges into the disciplinary knowledge discourses of modern scientific institutions. These representational strategies therefore generate/reproduce and validate disciplinary discourses about the other, constructing disciplinary 'regimes of truth'. In this way modern institutions appropriate and displace indigenous/local knowledges, silence the voices of local communities and regulate individual and community agency within a continuing subjugation of indigenous knowledges. This study reveals how working within modern institutions and disciplinary knowledges in participative education and development interactions can serve to implicate indigenous researchers in these institutional hegemonic processes. The study also notes evidence of a continued resistance to hegemonic Western knowledge discourses as indigenous communities have sustained many knowledge practices alongside Western knowledge discourses. There is also evidence of a recent emergence of counter-hegemonic indigenous knowledge discourses in environmental education and development practices in southern Africa. It is noted that these have been contingent upon the changing political terrain in southern Africa as this has opened the way for alternative discourses to the dominant conventional Western knowledges in formal education and development contexts. The counterhegemonic discourses invert power/knowledge relations, decentre hegemonic discourses and reposition indigenous knowledges in formal education and development contexts. This study suggests the need to foreground indigenous knowledges as a process of knowledge decolonisation that gives contextual and epistemic relevance to environmental education and development processes. This calls for a need for new strategies to transform existing institutions by creating enabling spaces for the representational inclusion of indigenous knowledges in formal/conventional knowledge discourses and their application in social contexts. This opens up possibilities for plural knowledge representations and for their integrative and reciprocal co-engagement in situated contexts of environmental education and development in southern Africa.
- Full Text:
- Date Issued: 2009
Insect pests of cultivated and wild olives, and some of their natural enemies, in the Eastern Cape, South Africa
- Authors: Mkize, Nolwazi
- Date: 2009
- Subjects: Pests -- Control -- South Africa -- Eastern Cape Olive -- Diseases and pests -- South Africa -- Eastern Cape Agriculture -- South Africa -- Eastern Cape Fruit-flies -- South Africa -- Eastern Cape Tephritidae Flea beetles Lace bugs
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5717 , http://hdl.handle.net/10962/d1005403
- Description: This thesis has two focuses. The first problem facing the olive industry in the Eastern Cape is the growers’ perceptions of both what the industry will provide them and what a pest management program might entail. The second focus is the biology of olive pests in the Eastern Cape in terms of understanding their populations and their natural enemies on private farms, with future hopes of understanding how Integrated Pest Management strategies can be developed for this crop. Eastern Cape private farmers, small-scale farmers and workers from agricultural training institutions were interviewed regarding the history and cultivation of the local olive crop. Only one commercially viable olive grove was identified; other groves were small, experimental pilot ventures. The introduction of olives to small-scale farmers and agricultural training schools was generally a top-down initiative that led to a lack of sense of ownership and the trees being neglected. Other problems included poor human capital; poor financial capital; lack of adequate support; lack of knowledge transfer and stability; lack of communication and evaluation procedures of the project; miscommunication; and finally, olive pests. Apart from hesitancy to plant at a commercial scale, the main problem facing private farmers (Varnam Farm, Hewlands Farm and Springvale Farm) was pests. Therefore an investigation of pests from private farms was conducted ranging from collection of cultivated and wild olive fruit and flea beetle larvae for parasitism, trapping systems both for fruit flies and olive flea beetle adults. A survey of olive fruits yielded larval fruit flies of the families Tephritidae (Bactrocera oleae (Rossi), B. biguttula (Bezzi) and Ceratitis capitata (Wiedemann)) and Drosophilidae (Drosophila melanogaster (Meigen)) from wild olives (O. europaea cuspidata (Wall. ex G. Don) Cif.) but none from cultivated olives (O. e. europaea L.). Braconid wasps (Opiinae and Braconinae) were reared only from fruits containing B. oleae and B. biguttula. This suggests that B. oleae is not of economic significance in the Eastern Cape, perhaps because it is controlled to a significant level by natural enemies, but B. biguttula may be a potential economic pest. A survey of adult fruit flies using ChamP traps baited with ammonium bicarbonate and spiroketal capsules and Sensus trap baited with methyl eugenol and Questlure confirmed the relative importance of B. biguttula over B. oleae. ChamP traps were over 50 times better than Sensus traps for mass trapping of B. biguttula but both were ineffective for trapping B. oleae and C. capitata. Six indigenous flea beetles of the genus Argopistes Motschulsky (Chrysomelidae: Alticinae) were found, three described by Bryant in 1922 and 1944 and three new species. Their morphology was investigated by scanning electron microscopy and mutivariate morphometric analysis. The leaf-mining larvae are pests of wild and cultivated olives in South Africa and threaten the local olive industry. At Springvale Farm, A. oleae Bryant and A. sexvittatus Bryant preferred the upper parts of trees, near new leaves. Pseudophanomeris inopinatus (Blkb.) (Braconidae) was reared from 23 Argopistes larvae. The beetle larvae might not be controlled to a significant level by natural enemies because the rate of parasitism was low. The olive flea beetles showed no attraction to traps containing various volatile compounds as baits. The lace bug, Plerochila australis Distant (Tingidae), was sometimes a pest. It showed a preference for the underside of leaves on the lower parts of the trees. A moth, Palpita unionalis Hübner (Crambidae), was reared in very low numbers and without parasitoids. A twig-boring beetle larva, chalcidoid parasitoids and seed wasps of the families Eurytomidae, Ormyridae and Eupelmidae were also recorded.
- Full Text:
- Date Issued: 2009
- Authors: Mkize, Nolwazi
- Date: 2009
- Subjects: Pests -- Control -- South Africa -- Eastern Cape Olive -- Diseases and pests -- South Africa -- Eastern Cape Agriculture -- South Africa -- Eastern Cape Fruit-flies -- South Africa -- Eastern Cape Tephritidae Flea beetles Lace bugs
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5717 , http://hdl.handle.net/10962/d1005403
- Description: This thesis has two focuses. The first problem facing the olive industry in the Eastern Cape is the growers’ perceptions of both what the industry will provide them and what a pest management program might entail. The second focus is the biology of olive pests in the Eastern Cape in terms of understanding their populations and their natural enemies on private farms, with future hopes of understanding how Integrated Pest Management strategies can be developed for this crop. Eastern Cape private farmers, small-scale farmers and workers from agricultural training institutions were interviewed regarding the history and cultivation of the local olive crop. Only one commercially viable olive grove was identified; other groves were small, experimental pilot ventures. The introduction of olives to small-scale farmers and agricultural training schools was generally a top-down initiative that led to a lack of sense of ownership and the trees being neglected. Other problems included poor human capital; poor financial capital; lack of adequate support; lack of knowledge transfer and stability; lack of communication and evaluation procedures of the project; miscommunication; and finally, olive pests. Apart from hesitancy to plant at a commercial scale, the main problem facing private farmers (Varnam Farm, Hewlands Farm and Springvale Farm) was pests. Therefore an investigation of pests from private farms was conducted ranging from collection of cultivated and wild olive fruit and flea beetle larvae for parasitism, trapping systems both for fruit flies and olive flea beetle adults. A survey of olive fruits yielded larval fruit flies of the families Tephritidae (Bactrocera oleae (Rossi), B. biguttula (Bezzi) and Ceratitis capitata (Wiedemann)) and Drosophilidae (Drosophila melanogaster (Meigen)) from wild olives (O. europaea cuspidata (Wall. ex G. Don) Cif.) but none from cultivated olives (O. e. europaea L.). Braconid wasps (Opiinae and Braconinae) were reared only from fruits containing B. oleae and B. biguttula. This suggests that B. oleae is not of economic significance in the Eastern Cape, perhaps because it is controlled to a significant level by natural enemies, but B. biguttula may be a potential economic pest. A survey of adult fruit flies using ChamP traps baited with ammonium bicarbonate and spiroketal capsules and Sensus trap baited with methyl eugenol and Questlure confirmed the relative importance of B. biguttula over B. oleae. ChamP traps were over 50 times better than Sensus traps for mass trapping of B. biguttula but both were ineffective for trapping B. oleae and C. capitata. Six indigenous flea beetles of the genus Argopistes Motschulsky (Chrysomelidae: Alticinae) were found, three described by Bryant in 1922 and 1944 and three new species. Their morphology was investigated by scanning electron microscopy and mutivariate morphometric analysis. The leaf-mining larvae are pests of wild and cultivated olives in South Africa and threaten the local olive industry. At Springvale Farm, A. oleae Bryant and A. sexvittatus Bryant preferred the upper parts of trees, near new leaves. Pseudophanomeris inopinatus (Blkb.) (Braconidae) was reared from 23 Argopistes larvae. The beetle larvae might not be controlled to a significant level by natural enemies because the rate of parasitism was low. The olive flea beetles showed no attraction to traps containing various volatile compounds as baits. The lace bug, Plerochila australis Distant (Tingidae), was sometimes a pest. It showed a preference for the underside of leaves on the lower parts of the trees. A moth, Palpita unionalis Hübner (Crambidae), was reared in very low numbers and without parasitoids. A twig-boring beetle larva, chalcidoid parasitoids and seed wasps of the families Eurytomidae, Ormyridae and Eupelmidae were also recorded.
- Full Text:
- Date Issued: 2009
Investigation of device and performance parameters of photovoltaic devices
- Macabebe, Erees Queen Barrido
- Authors: Macabebe, Erees Queen Barrido
- Date: 2009
- Subjects: Photovoltaic cells , Solar cells , Photovoltaic power systems , Photovoltaic power generation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10538 , http://hdl.handle.net/10948/1003 , http://hdl.handle.net/10948/d1012890 , Photovoltaic cells , Solar cells , Photovoltaic power systems , Photovoltaic power generation
- Description: In order to investigate the influence of parasitic resistances, saturation current and diode ideality factor on the performance of photovoltaic devices, parameter extraction routines employing the standard iteration (SI) method and the particle swarm optimization (PSO) method were developed to extract the series resistance, shunt resistance, saturation current and ideality factor from the I-V characteristics of solar cells and PV modules. The well-known one- and two-diode models were used to describe the behavior of the I-V curve and the parameters of the models were determined by approximation and iteration techniques. The SI and the PSO extraction programmes were used to assess the suitability of the one- and the two-diode solar cell models in describing the I-V characteristics of mono- and multicrystalline silicon solar cells, CISS- and CIGSS-based solar cells. This exercise revealed that the two-diode model provides more information regarding the different processes involved in solar cell operation. Between the two methods developed, the PSO method is faster, yielded fitted curves with lower standard deviation of residuals and, therefore, was the preferred extraction method. The PSO method was then used to extract the device parameters of CISS-based solar cells with the CISS layer selenized under different selenization process conditions and CIGSS-based solar cells with varying i-ZnO layer thickness. For the CISS-based solar cells, the detrimental effect of parasitic resistances on device performance increased when the temperature and duration of the selenization process was increased. For the CIGSS-based devices, photogeneration improved with increasing i-ZnO layer thickness. At high forward bias, bulk recombination and/or tunneling-assisted recombination were the dominant processes affecting the I-V characteristics of the devices. v Lastly, device and performance parameters of mono-, multicrystalline silicon and CIS modules derived from I-V characteristics obtained under dark and illuminated conditions were analyzed considering the effects of temperature on the performance of the devices. Results showed that the effects of parasitic resistances are greater under illumination and, under outdoor conditions, the values further declined due to increasing temperature. The saturation current and ideality factor also increased under outdoor conditions which suggest increased recombination and, coupled with the adverse effects of parasitic resistances, these factors result in lower FF and lower maximum power point. Analysis performed on crystalline silicon and thin film devices utilized in this study revealed that parameter extraction from I-V characteristics of photovoltaic devices and, in particular, the implementation of PSO in solar cell device parameter extraction developed in this work is a useful characterization technique.
- Full Text:
- Date Issued: 2009
- Authors: Macabebe, Erees Queen Barrido
- Date: 2009
- Subjects: Photovoltaic cells , Solar cells , Photovoltaic power systems , Photovoltaic power generation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10538 , http://hdl.handle.net/10948/1003 , http://hdl.handle.net/10948/d1012890 , Photovoltaic cells , Solar cells , Photovoltaic power systems , Photovoltaic power generation
- Description: In order to investigate the influence of parasitic resistances, saturation current and diode ideality factor on the performance of photovoltaic devices, parameter extraction routines employing the standard iteration (SI) method and the particle swarm optimization (PSO) method were developed to extract the series resistance, shunt resistance, saturation current and ideality factor from the I-V characteristics of solar cells and PV modules. The well-known one- and two-diode models were used to describe the behavior of the I-V curve and the parameters of the models were determined by approximation and iteration techniques. The SI and the PSO extraction programmes were used to assess the suitability of the one- and the two-diode solar cell models in describing the I-V characteristics of mono- and multicrystalline silicon solar cells, CISS- and CIGSS-based solar cells. This exercise revealed that the two-diode model provides more information regarding the different processes involved in solar cell operation. Between the two methods developed, the PSO method is faster, yielded fitted curves with lower standard deviation of residuals and, therefore, was the preferred extraction method. The PSO method was then used to extract the device parameters of CISS-based solar cells with the CISS layer selenized under different selenization process conditions and CIGSS-based solar cells with varying i-ZnO layer thickness. For the CISS-based solar cells, the detrimental effect of parasitic resistances on device performance increased when the temperature and duration of the selenization process was increased. For the CIGSS-based devices, photogeneration improved with increasing i-ZnO layer thickness. At high forward bias, bulk recombination and/or tunneling-assisted recombination were the dominant processes affecting the I-V characteristics of the devices. v Lastly, device and performance parameters of mono-, multicrystalline silicon and CIS modules derived from I-V characteristics obtained under dark and illuminated conditions were analyzed considering the effects of temperature on the performance of the devices. Results showed that the effects of parasitic resistances are greater under illumination and, under outdoor conditions, the values further declined due to increasing temperature. The saturation current and ideality factor also increased under outdoor conditions which suggest increased recombination and, coupled with the adverse effects of parasitic resistances, these factors result in lower FF and lower maximum power point. Analysis performed on crystalline silicon and thin film devices utilized in this study revealed that parameter extraction from I-V characteristics of photovoltaic devices and, in particular, the implementation of PSO in solar cell device parameter extraction developed in this work is a useful characterization technique.
- Full Text:
- Date Issued: 2009
Lead-radium dating of two deep-water fishes from the southern hemisphere, Patagonian toothfish (Dissostichus eleginoides) and Orange Roughy (Hoplostethus atlanticus)
- Authors: Andrews, Allen Hia
- Date: 2009
- Subjects: Fishes -- Age determination , Fishes -- Southern hemisphere -- Longevity , Fishes -- Growth , Radioactive dating , Patagonian toothfish , Patagonian toothfish -- Fisheries , Orange roughy , Orange roughy -- Fisheries , Deep-sea fishes -- Southern hemisphere , Deep-sea fisheries , Deep-sea fisheries -- Southern hemisphere
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5295 , http://hdl.handle.net/10962/d1005140
- Description: Patagonian toothfish (Dissostichus eleginoides) or "Chilean sea bass" support a valuable and controversial fishery, but the life history is little known and longevity estimates range from ~20 to more than 40 or 50 yr. In this study, lead-radium dating provided validated age estimates from juveniles to older adults, supporting the use of otoliths as accurate indicators of age. The oldest age groups were near 30 yr, which provided support for age estimates exceeding 40 or 50 yr from grow zone counts in otolith sections. Hence, scale reading, which rarely exceeds 20 years, has the potential for age underestimation. Lead-radium dating revealed what may be minor differences in age interpretation between two facilities and findings may provide an age-validated opportunity for the CCAMLR Otolith Network to reassess otolith interpretations. Orange roughy (Hoplostethus atlanticus) support a major deep-sea fishery and stock assessments often depend on age analyses, but lifespan estimates range from ~20 to over 100 yr and validation of growth zone counts remained unresolved. An early application of lead-radium dating supported centenarian ages, but the findings were met with disbelief and some studies have attempted to discredit the technique and the long lifespan. In this study, an improved lead-radium dating technique used smaller samples than previously possible and circumvented assumptions that were previously necessary. Lead-radium dating of otolith cores, the first few years of growth, provided ratios that correlated well with the ingrowth curve. This provided robust support for age estimates from otolith thin sections. Use of radiometric ages as independent age estimates indicated the fish in the oldest group were at least 93 yr. Lead-radium dating has validated a centenarian lifespan for orange roughy. To date, radium-226 has been measured in otoliths of 39 fish species ranging from the northern Pacific and Atlantic Oceans to the Southern Ocean. In total, 367 reliable radium-226 measurements were made in 36 studies since the first lead-radium dating study on fish in 1982. The activity of radium-226 measurements ranged over 3 orders of magnitude (<0.001 to >1.0 dpm.g⁻¹). An analysis revealed ontogenetic differences in radium-226 uptake that may be attributed to changes in habitat or diet. Radiometric age from otolith core studies was used to describe a radium-226 uptake time-series for some species, which revealed interesting patterns over long periods. This synopsis provides information on the uptake of radium-226 to otoliths from an environmental perspective, which can be used as a basis for future studies.
- Full Text:
- Date Issued: 2009
- Authors: Andrews, Allen Hia
- Date: 2009
- Subjects: Fishes -- Age determination , Fishes -- Southern hemisphere -- Longevity , Fishes -- Growth , Radioactive dating , Patagonian toothfish , Patagonian toothfish -- Fisheries , Orange roughy , Orange roughy -- Fisheries , Deep-sea fishes -- Southern hemisphere , Deep-sea fisheries , Deep-sea fisheries -- Southern hemisphere
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5295 , http://hdl.handle.net/10962/d1005140
- Description: Patagonian toothfish (Dissostichus eleginoides) or "Chilean sea bass" support a valuable and controversial fishery, but the life history is little known and longevity estimates range from ~20 to more than 40 or 50 yr. In this study, lead-radium dating provided validated age estimates from juveniles to older adults, supporting the use of otoliths as accurate indicators of age. The oldest age groups were near 30 yr, which provided support for age estimates exceeding 40 or 50 yr from grow zone counts in otolith sections. Hence, scale reading, which rarely exceeds 20 years, has the potential for age underestimation. Lead-radium dating revealed what may be minor differences in age interpretation between two facilities and findings may provide an age-validated opportunity for the CCAMLR Otolith Network to reassess otolith interpretations. Orange roughy (Hoplostethus atlanticus) support a major deep-sea fishery and stock assessments often depend on age analyses, but lifespan estimates range from ~20 to over 100 yr and validation of growth zone counts remained unresolved. An early application of lead-radium dating supported centenarian ages, but the findings were met with disbelief and some studies have attempted to discredit the technique and the long lifespan. In this study, an improved lead-radium dating technique used smaller samples than previously possible and circumvented assumptions that were previously necessary. Lead-radium dating of otolith cores, the first few years of growth, provided ratios that correlated well with the ingrowth curve. This provided robust support for age estimates from otolith thin sections. Use of radiometric ages as independent age estimates indicated the fish in the oldest group were at least 93 yr. Lead-radium dating has validated a centenarian lifespan for orange roughy. To date, radium-226 has been measured in otoliths of 39 fish species ranging from the northern Pacific and Atlantic Oceans to the Southern Ocean. In total, 367 reliable radium-226 measurements were made in 36 studies since the first lead-radium dating study on fish in 1982. The activity of radium-226 measurements ranged over 3 orders of magnitude (<0.001 to >1.0 dpm.g⁻¹). An analysis revealed ontogenetic differences in radium-226 uptake that may be attributed to changes in habitat or diet. Radiometric age from otolith core studies was used to describe a radium-226 uptake time-series for some species, which revealed interesting patterns over long periods. This synopsis provides information on the uptake of radium-226 to otoliths from an environmental perspective, which can be used as a basis for future studies.
- Full Text:
- Date Issued: 2009
Learning, governance and livelihoods : toward adaptive co-management under resource poor conditions in South Africa
- Authors: Cundill, Georgina
- Date: 2009
- Subjects: Natural resources -- Co-management -- South Africa Rural poor -- South Africa Rural development -- South Africa Households -- Economic aspects -- South Africa Sustainable development -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4747 , http://hdl.handle.net/10962/d1006961
- Description: Through collaborative monitoring and case study comparison, this thesis explores conceptual and methodological approaches to monitoring transitions toward adaptive co-management. In so doing, a number of knowledge gaps are addressed. Firstly, conceptual and methodological frameworks are developed for monitoring transitions toward adaptive co-management. Secondly, a conceptual and practical approach to monitoring the processes of collaboration and learning is developed and tested. Thirdly, a conceptual and practical approach to monitoring the governance outcomes of adaptive co-management is developed and tested. Fourthly, a conceptual and practical approach to monitoring the livelihood outcomes of adaptive co-management is developed and tested. Based on the outcomes from these four components of the study, this thesis explores the ways in which transitions toward adaptive co-management might be initiated under the resource poor conditions that characterise South Africa's communal areas. The four case studies explored in the study are described as 'resource poor' in terms of institutional capacity, ecosystem productivity and social vulnerability. From a resilience perspective these case studies can be described as being in the re-organisation phase of the adaptive cycle following multiple disturbances over time, largely due to South Africa's historical 'separate development' policies. Scholars have suggested that it is in this re-organisation phase that innovation and novelty might occur. The lens of social learning is applied to analyse collaborative processes within these contexts. Results indicate that the institutional innovation necessary for transitions toward adaptive co-management relies on careful facilitation by an 'honest broker'. Equally important is finding a balance between maintaining key individuals and knowledge holders within decision making networks, and preventing rigidity and vulnerability within communities of practice. The results point to an over simplification in the rhetoric that currently surrounds the learning outcomes of multi level networks. The governance outcomes of the initiatives are explored through the lenses of adaptive governance, social capital, adaptive capacity and self-organisation. Results indicate that under resource poor conditions creating the conditions that facilitate self-organisation is the major challenge facing transformations toward adaptive governance. Long term access to reliable information and capacity and financial support for adaptive management are key constraining variables. The livelihood outcomes of the initiatives are analysed through the lens of resilience and diversification. Results suggest that flexibility, rather than livelihood diversity, is the key livelihood strategy employed by households in situations were options are limited. Interventions that enhance opportunities for households to specialise in situ by actively dealing with structural constraints, such as access to markets and credit, is vital to encouraging innovation during transitions toward adaptive co-management. Based on the results from monitoring, this study identifies key focus areas that require a great deal more attention if transitions toward adaptive co-management are to be initiated under resource poor conditions.
- Full Text:
- Date Issued: 2009
- Authors: Cundill, Georgina
- Date: 2009
- Subjects: Natural resources -- Co-management -- South Africa Rural poor -- South Africa Rural development -- South Africa Households -- Economic aspects -- South Africa Sustainable development -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4747 , http://hdl.handle.net/10962/d1006961
- Description: Through collaborative monitoring and case study comparison, this thesis explores conceptual and methodological approaches to monitoring transitions toward adaptive co-management. In so doing, a number of knowledge gaps are addressed. Firstly, conceptual and methodological frameworks are developed for monitoring transitions toward adaptive co-management. Secondly, a conceptual and practical approach to monitoring the processes of collaboration and learning is developed and tested. Thirdly, a conceptual and practical approach to monitoring the governance outcomes of adaptive co-management is developed and tested. Fourthly, a conceptual and practical approach to monitoring the livelihood outcomes of adaptive co-management is developed and tested. Based on the outcomes from these four components of the study, this thesis explores the ways in which transitions toward adaptive co-management might be initiated under the resource poor conditions that characterise South Africa's communal areas. The four case studies explored in the study are described as 'resource poor' in terms of institutional capacity, ecosystem productivity and social vulnerability. From a resilience perspective these case studies can be described as being in the re-organisation phase of the adaptive cycle following multiple disturbances over time, largely due to South Africa's historical 'separate development' policies. Scholars have suggested that it is in this re-organisation phase that innovation and novelty might occur. The lens of social learning is applied to analyse collaborative processes within these contexts. Results indicate that the institutional innovation necessary for transitions toward adaptive co-management relies on careful facilitation by an 'honest broker'. Equally important is finding a balance between maintaining key individuals and knowledge holders within decision making networks, and preventing rigidity and vulnerability within communities of practice. The results point to an over simplification in the rhetoric that currently surrounds the learning outcomes of multi level networks. The governance outcomes of the initiatives are explored through the lenses of adaptive governance, social capital, adaptive capacity and self-organisation. Results indicate that under resource poor conditions creating the conditions that facilitate self-organisation is the major challenge facing transformations toward adaptive governance. Long term access to reliable information and capacity and financial support for adaptive management are key constraining variables. The livelihood outcomes of the initiatives are analysed through the lens of resilience and diversification. Results suggest that flexibility, rather than livelihood diversity, is the key livelihood strategy employed by households in situations were options are limited. Interventions that enhance opportunities for households to specialise in situ by actively dealing with structural constraints, such as access to markets and credit, is vital to encouraging innovation during transitions toward adaptive co-management. Based on the results from monitoring, this study identifies key focus areas that require a great deal more attention if transitions toward adaptive co-management are to be initiated under resource poor conditions.
- Full Text:
- Date Issued: 2009
Mathematical requirements for first-year BCOM students at NMMU
- Authors: Walton, Marguerite
- Date: 2009
- Subjects: Business mathematics -- South Africa , Mathematics -- Study and teaching (Higher) -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10502 , http://hdl.handle.net/10948/886 , Business mathematics -- South Africa , Mathematics -- Study and teaching (Higher) -- South Africa
- Description: These studies have focused on identifying the mathematical requirements of first-year BCom students at Nelson Mandela Metropolitan University. The research methodology used in this quantitative study was to make use of interviewing, questionnaire investigation, and document analysis in the form of textbook, test and examination analysis. These methods provided data that fitted into a grounded theory approach. The study concluded by identifying the list of mathematical topics required for the first year of the core subjects in the BCom degree programme. In addition, the study found that learners who study Mathematics in the National Senior Certificate should be able to cope with the mathematical content included in their BCom degree programme, while learners studying Mathematical Literacy would probably need support in some of the areas of mathematics, especially algebra, in order to cope with the mathematical content included in their BCom degree programme. It makes a valuable contribution towards elucidating the mathematical requirements needed to improve the chances of successful BCom degree programme studies at South African universities. It also draws the contours for starting to design an efficient support course for future “at-risk” students who enter higher education studies.
- Full Text:
- Date Issued: 2009
- Authors: Walton, Marguerite
- Date: 2009
- Subjects: Business mathematics -- South Africa , Mathematics -- Study and teaching (Higher) -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10502 , http://hdl.handle.net/10948/886 , Business mathematics -- South Africa , Mathematics -- Study and teaching (Higher) -- South Africa
- Description: These studies have focused on identifying the mathematical requirements of first-year BCom students at Nelson Mandela Metropolitan University. The research methodology used in this quantitative study was to make use of interviewing, questionnaire investigation, and document analysis in the form of textbook, test and examination analysis. These methods provided data that fitted into a grounded theory approach. The study concluded by identifying the list of mathematical topics required for the first year of the core subjects in the BCom degree programme. In addition, the study found that learners who study Mathematics in the National Senior Certificate should be able to cope with the mathematical content included in their BCom degree programme, while learners studying Mathematical Literacy would probably need support in some of the areas of mathematics, especially algebra, in order to cope with the mathematical content included in their BCom degree programme. It makes a valuable contribution towards elucidating the mathematical requirements needed to improve the chances of successful BCom degree programme studies at South African universities. It also draws the contours for starting to design an efficient support course for future “at-risk” students who enter higher education studies.
- Full Text:
- Date Issued: 2009
Myth, Music & Modernism: the Wagnerian dimension in Virginia Woolf's Mrs Dalloway and the waves and James Joyce’s Finnegans Wake
- Authors: McGregor, Jamie Alexander
- Date: 2009
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/77069 , vital:30662
- Description: The study of Wagner's influence on the modernist novel is an established field with clear room for further contributions. Very little of the criticism undertaken to date takes full cognizance of the philosophical content of Wagner's dramas: a revolutionary form of romanticism that calls into question the very nature of the world, its most radical component being Schopenhauer's version of transcendental idealism. The compatibility of this doctrine with Wagner's earlier work, with its already marked privileging of myth over history, enabled his later dramas, consciously influenced by Schopenhauer, to crown a body of work greater than the sum of its parts. In works by Virginia Woolf and James Joyce, the "translation" of Wagnerian ideas into novelistic form demonstrates how they might be applied in "real life". In Mrs Dalloway, the figure of Septimus can be read as partly modelled on Wagner's heroes Siegfried and Tristan, two outstanding examples of the opposing heroic types found throughout his oeuvre, whose contrasting attributes are fused in Septimus's bipolar personality. The Wagnerian pattern also throws light on Septimus's transcendental "relationship" with a woman he does not even know, and on the implied noumenal identity of seemingly isolated individuals. In The Waves, the allusions to both Parsifal and the Ring need to be reconsidered in light of the fact that these works' heroes are all but identical (a fact overlooked in previous criticism); as Wagner's solar hero par excellence, Siegfried is central to the novel's cyclical symbolism. The Waves also revisits the question of identity but in a more cosmic context – the metaphysical unity of everything. In Finnegans Wake, the symbolism of the cosmic cycle is again related to the Ring, as are Wagner's two heroic types to the Shem / Shaun opposition (the Joyce / Woolf parallels here have also been overlooked in criticism to date). All three texts reveal a fascination with the two contrasting faces of a Wagnerian hero who embodies the dual nature of reality, mirroring in himself the eternal rise and fall of world history and, beyond them, the timeless stasis of myth.
- Full Text:
- Date Issued: 2009
- Authors: McGregor, Jamie Alexander
- Date: 2009
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/77069 , vital:30662
- Description: The study of Wagner's influence on the modernist novel is an established field with clear room for further contributions. Very little of the criticism undertaken to date takes full cognizance of the philosophical content of Wagner's dramas: a revolutionary form of romanticism that calls into question the very nature of the world, its most radical component being Schopenhauer's version of transcendental idealism. The compatibility of this doctrine with Wagner's earlier work, with its already marked privileging of myth over history, enabled his later dramas, consciously influenced by Schopenhauer, to crown a body of work greater than the sum of its parts. In works by Virginia Woolf and James Joyce, the "translation" of Wagnerian ideas into novelistic form demonstrates how they might be applied in "real life". In Mrs Dalloway, the figure of Septimus can be read as partly modelled on Wagner's heroes Siegfried and Tristan, two outstanding examples of the opposing heroic types found throughout his oeuvre, whose contrasting attributes are fused in Septimus's bipolar personality. The Wagnerian pattern also throws light on Septimus's transcendental "relationship" with a woman he does not even know, and on the implied noumenal identity of seemingly isolated individuals. In The Waves, the allusions to both Parsifal and the Ring need to be reconsidered in light of the fact that these works' heroes are all but identical (a fact overlooked in previous criticism); as Wagner's solar hero par excellence, Siegfried is central to the novel's cyclical symbolism. The Waves also revisits the question of identity but in a more cosmic context – the metaphysical unity of everything. In Finnegans Wake, the symbolism of the cosmic cycle is again related to the Ring, as are Wagner's two heroic types to the Shem / Shaun opposition (the Joyce / Woolf parallels here have also been overlooked in criticism to date). All three texts reveal a fascination with the two contrasting faces of a Wagnerian hero who embodies the dual nature of reality, mirroring in himself the eternal rise and fall of world history and, beyond them, the timeless stasis of myth.
- Full Text:
- Date Issued: 2009
New platinum coordination compounds : their synthesis, characterization and anticancer application
- Authors: Oosthuizen, Lukas Marthinus
- Date: 2009
- Subjects: Platinum compounds , Antineoplastic agents
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10430 , http://hdl.handle.net/10948/d1018795
- Description: The aim of this thesis was to investigate the properties of novel platinum compounds with possible potential as anticancer agents, and to determine how their behaviour could lead to a better understanding of the chemistry involved. The final criteria were improvement of their anticancer behaviour. Since many questions are still unanswered as to the role of sulfur in anticancer action, studies were undertaken to synthesize novel platinum(II) complexes having non-leaving groups consisting of a combination of an aromatic nitrogen and thioetherial sulfur capable of forming a five membered ring upon coordination. The structural unit was 1-methyl-2-methylthioalkyl/aryl. Numerous complexes formed by these ligands each having chloro, bromo, iodo and oxalato leaving groups were then fully characterized. The results obtained by the various synthetic methods were compared and explained in terms of the chemistry involved. The role of the sulfur donor was indicated in both the halo- and oxalato-complexes and proved to be strongly influenced by the nature of the leaving groups. Their differences are reflected in their anticancer behaviour. The study was extended to mononitroplatinum(IV) complexes, in view of the kinetically stable platinum(IV) compounds and advantages related to this. A specific mononitroplatinum(IV) complex which proved to have good anticancer and STAT 3 properties could according to the literature not be synthesized successfully in a good yield and a high degree of purity. The results of extensive studies showed that the main problem centred around the simultaneous reactions in equilibrium during the synthesis. A number of these species formed as a result of side reactions could be identified and their close separation factors indicated chromatographically. The mechanism of these reactions and the unstable intermediate species involved could be rationalized and compared to analogues in the literature. All the complexes studied were characterized by spectral and thermal methods both in solution as well as the solid state. Their anticancer behaviour towards three anticancer cell lines (Hela, MCF 7, Ht 29) were determined and acted as a guide towards possible structural modifications for their improved capability. Three crystal structures of platinum(II) complexes were determined. The extent of the ionization of the platinum(II) complexes as well the redox potentials (Pt(II) / Pt(IV)) of the platinum(IV) complexes were particularly important factors pertaining to their anticancer action.
- Full Text:
- Date Issued: 2009
- Authors: Oosthuizen, Lukas Marthinus
- Date: 2009
- Subjects: Platinum compounds , Antineoplastic agents
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10430 , http://hdl.handle.net/10948/d1018795
- Description: The aim of this thesis was to investigate the properties of novel platinum compounds with possible potential as anticancer agents, and to determine how their behaviour could lead to a better understanding of the chemistry involved. The final criteria were improvement of their anticancer behaviour. Since many questions are still unanswered as to the role of sulfur in anticancer action, studies were undertaken to synthesize novel platinum(II) complexes having non-leaving groups consisting of a combination of an aromatic nitrogen and thioetherial sulfur capable of forming a five membered ring upon coordination. The structural unit was 1-methyl-2-methylthioalkyl/aryl. Numerous complexes formed by these ligands each having chloro, bromo, iodo and oxalato leaving groups were then fully characterized. The results obtained by the various synthetic methods were compared and explained in terms of the chemistry involved. The role of the sulfur donor was indicated in both the halo- and oxalato-complexes and proved to be strongly influenced by the nature of the leaving groups. Their differences are reflected in their anticancer behaviour. The study was extended to mononitroplatinum(IV) complexes, in view of the kinetically stable platinum(IV) compounds and advantages related to this. A specific mononitroplatinum(IV) complex which proved to have good anticancer and STAT 3 properties could according to the literature not be synthesized successfully in a good yield and a high degree of purity. The results of extensive studies showed that the main problem centred around the simultaneous reactions in equilibrium during the synthesis. A number of these species formed as a result of side reactions could be identified and their close separation factors indicated chromatographically. The mechanism of these reactions and the unstable intermediate species involved could be rationalized and compared to analogues in the literature. All the complexes studied were characterized by spectral and thermal methods both in solution as well as the solid state. Their anticancer behaviour towards three anticancer cell lines (Hela, MCF 7, Ht 29) were determined and acted as a guide towards possible structural modifications for their improved capability. Three crystal structures of platinum(II) complexes were determined. The extent of the ionization of the platinum(II) complexes as well the redox potentials (Pt(II) / Pt(IV)) of the platinum(IV) complexes were particularly important factors pertaining to their anticancer action.
- Full Text:
- Date Issued: 2009
Patterns and determinants of species richness in mesic temparate grasslands of South Africa
- Authors: Hoare, David Barry
- Date: 2009
- Subjects: Grassland ecology -- South Africa -- Eastern Cape , Plant diversity -- South Africa -- Eastern Cape , Vegetation and climate
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10613 , http://hdl.handle.net/10948/1275 , Grassland ecology -- South Africa -- Eastern Cape , Plant diversity -- South Africa -- Eastern Cape , Vegetation and climate
- Description: The aim of this study is to gain a predictive understanding of the patterns and determinants of plant biodiversity in temperate, mesic grasslands of South Africa with a primary focus on the geographical area of the Eastern Cape. From a review of the literature on hypotheses explaining diversity (Chapter 2) it was possible to formulate a number of hypotheses that could be tested to explain species richness patterns in Eastern Cape grasslands. This thesis is organised so that each main chapter deals with a specific body of theory concerning the explanation of diversity patterns. A detailed description of the study area is provided (Chapter 3), including environmental variation and a description of major vegetation patterns. A summary is provided of grassland plant community patterns, as determined by phytosociological studies in the study area. A multivariate analysis of environmental variables was undertaken to determine which variables contributed the most towards explaining environmental variation in the study area and to determine whether any variables co-vary, a possible problem for any multivariate analysis in later chapters. Altitude produced one of the strongest gradients in the study area. There were a number of variables that were correlated with altitude, most notably temperature. Rainfall co-varied partially with altitude, but there was also a strong rainfall gradient perpendicular to the altitude gradient. A description of species richness, diversity and evenness patterns at the plot scale within different grassland plant communities of the Eastern Cape is provided in Chapter 4. To determine whether the environment acts differently on different growth forms, the contribution to species richness by different major growth forms is analysed. Furthermore, since the majority of literature attempts to explain diversity in terms of environmental factors, it was necessary to analyse the relationship between species richness and various environmental variables. The results indicate that there is high variation in species richness both within and among grassland communities. Forbs make the most significant contribution to overall species richness per 100 m2, followed by grasses. Variance in richness of all species together is not significantly related to environmental variables in mesic grasslands, but is significantly related to environmental variables in semi-arid grasslands. The result of greatest interest from this chapter is the fact that richness amongst different life-forms in the same place is explained by different environmental factors, indicating that the environmental factors that affect coexistence of species have a different effect on different life-forms. A classification of all the species of the dataset into plant functional types using a multivariate approach based on functional traits was conducted (Chapter 5). The grass species were classified into 16 functional types and the forbs into 14 functional types. The functional type classification provided the opportunity for undertaking analyses to develop an understanding of 8 the contribution by niche differentiation towards promoting species richness (Chapter 6). The results provide evidence of niche differentiation in the grasslands of the study area and also that niche differentiation promotes species richness in the grasslands of the study area. It was found that higher rainfall grasslands are less structured by niche differentiation than semi-arid grasslands. A regional / historical analysis is undertaken (Chapter 7) to investigate the relationship between the regional species pool and local richness, and the relationship between local richness and phytochorological diversity. Regional richness appears to have little effect in promoting local richness in grassland plant communities of the study area except at sites where there is high local richness. This provides an indication that regional richness only promotes local richness in the absence of local limiting factors. Phytochorological diversity promotes local richness, but mostly through diversity amongst species with narrow distribution ranges. Some theories ascertain that seasonal uncertainty may provide opportunities to species that would otherwise be outcompeted and thereby promote local richness. The degree to which seasonal uncertainty and seasonality promote local richness in the Eastern Cape grasslands was therefore investigated (Chapter 8). A weak relationship exists between these variables and local species richness in grassland communities of the study area, indicating that they do not promote niche differentiation to a significant degree in the study area. It is clear that in the grasslands of the Eastern Cape, environmental limiting factors are more important in semi-arid grasslands and species interactions are more important in mesic grasslands for structuring plant communities (Chapter 9, Discussion). Regional processes do not appear to be important in structuring local communities, but the analysis undertaken in this study shows that they may be significant when factors operating at the other two levels are overcome (species interactions and environmental limiting factors.
- Full Text:
- Date Issued: 2009
- Authors: Hoare, David Barry
- Date: 2009
- Subjects: Grassland ecology -- South Africa -- Eastern Cape , Plant diversity -- South Africa -- Eastern Cape , Vegetation and climate
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10613 , http://hdl.handle.net/10948/1275 , Grassland ecology -- South Africa -- Eastern Cape , Plant diversity -- South Africa -- Eastern Cape , Vegetation and climate
- Description: The aim of this study is to gain a predictive understanding of the patterns and determinants of plant biodiversity in temperate, mesic grasslands of South Africa with a primary focus on the geographical area of the Eastern Cape. From a review of the literature on hypotheses explaining diversity (Chapter 2) it was possible to formulate a number of hypotheses that could be tested to explain species richness patterns in Eastern Cape grasslands. This thesis is organised so that each main chapter deals with a specific body of theory concerning the explanation of diversity patterns. A detailed description of the study area is provided (Chapter 3), including environmental variation and a description of major vegetation patterns. A summary is provided of grassland plant community patterns, as determined by phytosociological studies in the study area. A multivariate analysis of environmental variables was undertaken to determine which variables contributed the most towards explaining environmental variation in the study area and to determine whether any variables co-vary, a possible problem for any multivariate analysis in later chapters. Altitude produced one of the strongest gradients in the study area. There were a number of variables that were correlated with altitude, most notably temperature. Rainfall co-varied partially with altitude, but there was also a strong rainfall gradient perpendicular to the altitude gradient. A description of species richness, diversity and evenness patterns at the plot scale within different grassland plant communities of the Eastern Cape is provided in Chapter 4. To determine whether the environment acts differently on different growth forms, the contribution to species richness by different major growth forms is analysed. Furthermore, since the majority of literature attempts to explain diversity in terms of environmental factors, it was necessary to analyse the relationship between species richness and various environmental variables. The results indicate that there is high variation in species richness both within and among grassland communities. Forbs make the most significant contribution to overall species richness per 100 m2, followed by grasses. Variance in richness of all species together is not significantly related to environmental variables in mesic grasslands, but is significantly related to environmental variables in semi-arid grasslands. The result of greatest interest from this chapter is the fact that richness amongst different life-forms in the same place is explained by different environmental factors, indicating that the environmental factors that affect coexistence of species have a different effect on different life-forms. A classification of all the species of the dataset into plant functional types using a multivariate approach based on functional traits was conducted (Chapter 5). The grass species were classified into 16 functional types and the forbs into 14 functional types. The functional type classification provided the opportunity for undertaking analyses to develop an understanding of 8 the contribution by niche differentiation towards promoting species richness (Chapter 6). The results provide evidence of niche differentiation in the grasslands of the study area and also that niche differentiation promotes species richness in the grasslands of the study area. It was found that higher rainfall grasslands are less structured by niche differentiation than semi-arid grasslands. A regional / historical analysis is undertaken (Chapter 7) to investigate the relationship between the regional species pool and local richness, and the relationship between local richness and phytochorological diversity. Regional richness appears to have little effect in promoting local richness in grassland plant communities of the study area except at sites where there is high local richness. This provides an indication that regional richness only promotes local richness in the absence of local limiting factors. Phytochorological diversity promotes local richness, but mostly through diversity amongst species with narrow distribution ranges. Some theories ascertain that seasonal uncertainty may provide opportunities to species that would otherwise be outcompeted and thereby promote local richness. The degree to which seasonal uncertainty and seasonality promote local richness in the Eastern Cape grasslands was therefore investigated (Chapter 8). A weak relationship exists between these variables and local species richness in grassland communities of the study area, indicating that they do not promote niche differentiation to a significant degree in the study area. It is clear that in the grasslands of the Eastern Cape, environmental limiting factors are more important in semi-arid grasslands and species interactions are more important in mesic grasslands for structuring plant communities (Chapter 9, Discussion). Regional processes do not appear to be important in structuring local communities, but the analysis undertaken in this study shows that they may be significant when factors operating at the other two levels are overcome (species interactions and environmental limiting factors.
- Full Text:
- Date Issued: 2009
Photophysical and photochemical behaviour of metallophthalocyanines effect of nanoparticles and molecules of biological importance
- Authors: Idowu, Mopelola Abidemi
- Date: 2009
- Subjects: Electrochemistry Phthalocyanines Nanoparticles
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4329 , http://hdl.handle.net/10962/d1004990
- Description: Syntheses, spectral, photophysical and photochemical studies of some neutral, anionic and cationic metallophthalocyanine derivatives are presented. The effects of central metal ions, solvents, aggregation, surfactant, nanoparticles and bovine serum albumin on the photophysical and photochemical behaviour are investigated. Mercaptocarboxylic acid stabilized CdTe quantum dots (QDs) were used as energy donors to anionic water-soluble MPcs through Förster resonance energy transfer (FRET). Energy transfer (ET) from the QDs to the MPcs occurred upon photoexcitation of the QDs. An enhancement in efficiency of ET with the nature of the cappings on the QDs was observed with few occurrences of a non-Förster type ET. QDs were found to improve the photophysicochemical behaviour of the MPcs, with the possibility of indirect production of singlet oxygen (Φ[subscript Δ]) via FRET mechanism. Interaction of the QDs with cationic water-souble MPcs produced ion-pair complexes resulting in aggregates due to strong electronic coupling. The stoichiometry of the reaction and association constants are evaluated by the continuous variation method. Improved photophysicochemical behaviour with no spectral alterations was observed in MPcs in the presence of magnetic fluid. Complexes showed high triplet quantum yields with corresponding long lifetimes and high photostability. Elucidation of the results of the interaction of bovine serum albumin (BSA) with MPcs or QDs is presented. Increased efficiency of Φ[subscript Δ] generation of MPcs in the presence of BSA coupled with large binding constants, suggesting strong interaction of the MPcs with BSA was observed. Enhanced emission intensity of QDs when linked to or in a mixture with BSA due to radiationless recombination at the surface vacancies was also observed. The study revealed positive deviation from Stern-Volmer relationship suggesting the occurrence of static and dynamic mechanisms of quenching together. Fluorescence quenching of the MPcs by benzoquinone, analysed by Stern-Volmer relationship is also presented; the results were employed in determining fluorescence lifetimes of the complexes. Photoelectrochemical characteristics of MPc-sensitized electrodeposited ZnO thin films were studied; ZnOCPc / ZnO films have been improved to an incident photon-to-currentconversion (IPCE) value of 31.1 % with an absorbed photon-to-current conversion (APCE) of 59.6 %. The best obtained so far with phthalocyanine-type sensitizers on nanocrystalline ZnO films. Fluorescent-magnetic nanocomposite with excellent photophysical properties which can be exploited for combined photodynamic and hyperthermia therapies is also presented.
- Full Text:
- Date Issued: 2009
- Authors: Idowu, Mopelola Abidemi
- Date: 2009
- Subjects: Electrochemistry Phthalocyanines Nanoparticles
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4329 , http://hdl.handle.net/10962/d1004990
- Description: Syntheses, spectral, photophysical and photochemical studies of some neutral, anionic and cationic metallophthalocyanine derivatives are presented. The effects of central metal ions, solvents, aggregation, surfactant, nanoparticles and bovine serum albumin on the photophysical and photochemical behaviour are investigated. Mercaptocarboxylic acid stabilized CdTe quantum dots (QDs) were used as energy donors to anionic water-soluble MPcs through Förster resonance energy transfer (FRET). Energy transfer (ET) from the QDs to the MPcs occurred upon photoexcitation of the QDs. An enhancement in efficiency of ET with the nature of the cappings on the QDs was observed with few occurrences of a non-Förster type ET. QDs were found to improve the photophysicochemical behaviour of the MPcs, with the possibility of indirect production of singlet oxygen (Φ[subscript Δ]) via FRET mechanism. Interaction of the QDs with cationic water-souble MPcs produced ion-pair complexes resulting in aggregates due to strong electronic coupling. The stoichiometry of the reaction and association constants are evaluated by the continuous variation method. Improved photophysicochemical behaviour with no spectral alterations was observed in MPcs in the presence of magnetic fluid. Complexes showed high triplet quantum yields with corresponding long lifetimes and high photostability. Elucidation of the results of the interaction of bovine serum albumin (BSA) with MPcs or QDs is presented. Increased efficiency of Φ[subscript Δ] generation of MPcs in the presence of BSA coupled with large binding constants, suggesting strong interaction of the MPcs with BSA was observed. Enhanced emission intensity of QDs when linked to or in a mixture with BSA due to radiationless recombination at the surface vacancies was also observed. The study revealed positive deviation from Stern-Volmer relationship suggesting the occurrence of static and dynamic mechanisms of quenching together. Fluorescence quenching of the MPcs by benzoquinone, analysed by Stern-Volmer relationship is also presented; the results were employed in determining fluorescence lifetimes of the complexes. Photoelectrochemical characteristics of MPc-sensitized electrodeposited ZnO thin films were studied; ZnOCPc / ZnO films have been improved to an incident photon-to-currentconversion (IPCE) value of 31.1 % with an absorbed photon-to-current conversion (APCE) of 59.6 %. The best obtained so far with phthalocyanine-type sensitizers on nanocrystalline ZnO films. Fluorescent-magnetic nanocomposite with excellent photophysical properties which can be exploited for combined photodynamic and hyperthermia therapies is also presented.
- Full Text:
- Date Issued: 2009
Polarization mode dispersion emulation and the impact of high first-order PMD segments in optical telecommunication systems
- Authors: Musara, Vitalis
- Date: 2009
- Subjects: Optical communications , Fiber optics , Polarization (Light)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10519 , http://hdl.handle.net/10948/1138 , Optical communications , Fiber optics , Polarization (Light)
- Description: In this study, focus is centred on the measurement and emulation of first-order (FO-) and second-order (SO-) polarization mode dispersion (PMD). PMD has deleterious effects on the performance of high speed optical transmission network systems from 10 Gb/s and above. The first step was characterising deployed fibres for PMD and monitoring the state of polarization (SOP) light experiences as it propagates through the fibre. The PMD and SOP changes in deployed fibres were stochastic due to varying intrinsic and extrinsic perturbation changes. To fully understand the PMD phenomenon in terms of measurement accuracy, its complex behaviour, its implications, mitigation and compensation, PMD emulation is crucial. This thesis presents emulator designs which fall into different emulator categories. The key to these designs were the PMD equations and background on the PMD phenomenon. The cross product from the concatenation equation was applied in order to determine the coupling angle β (between 0o and 180o) that results in the SO-PMD of the emulator designs to be either adjustable or fixed. The digital delay line (DDL) or single polarization maintaining fibre (PMF) section was used to give a certain amount of FO-PMD but negligible SO-PMD. PMF sections (birefringent sections) were concatenated together to ensure FO- and SO-PMD coexist, emulating deployed fibres. FO- and SO-PMD can be controlled by altering mode coupling (coupling angles) and birefringence distribution. Emulators with PMD statistics approaching the theoretical distributions had high random coupling and several numbers of randomly distributed PMF sections. In addition, the lengths of their PMF sections lie within 20% standard deviation of the mean emulator length. Those emulators with PMD statistics that did not approach the theoretical distributions had limited numbers of randomly distributed PMF sections and mode coupling. Results also show that even when an emulator has high random mode coupling and several numbers of randomly distributed PMFs, its PMD statistics deviates away from expected theoretical distributions in the presence of polarization dependent loss (PDL). The emulators showed that the background autocorrelation function (BACF) approaches zero with increasing number of randomly mode coupled fibre sections. A zero BACF signifies that an emulator has large numbers of randomly distributed PMF sections and its presence means the opposite. The availability of SO-PMD in the emulators made the autocorrelation function (ACF) x asymmetric. In the absence of SO-PMD the ACF for a PMD emulator is symmetric. SO-PMD has no effect on the BACF. Polarization-optical time domain reflectometry (P-OTDR) measurements have shown that certain fibre sections along fibre link lengths have higher FO-PMD (HiFO-PMD) than other sections. This study investigates the impact of a HiFO-PMD section on the overall FO- and SO-PMD, the output state of polarization (SOP) and system performance on deployed fibres (through emulation). Results show that when the wavelength-independent FO-PMD vector of the HiFO-PMD section is greater than the FO-PMD contributions from the rest of the fibre link, the mean FO-PMD of the entire link is biased towards that of the HiFO-PMD section and the SO-PMD increases (β ≠ 0o or 180o) or remains fixed (β = 0o or 180o) depending on the coupling angle β between the HiFO-PMD section and the rest of the fibre link. In addition, the FO-PMD statistics deviates away from the theoretical Maxwellian distribution. However, experimental results show that the HiFO-PMD section has negligible influence on the SOPMD statistical distribution. An increase in the amount of FO-PMD on a HiFO-PMD section reduces the output SOP spread to a given minimum, in this study the minimum was reached when the HiFO-PMD ≥ 35 ps. However, the outcome of the output SOP spread depends on the location of the HiFO-PMD section along the fibre link length. It was found that when the HiFO-PMD section introduces SO-PMD, the bit error rate (BER) is much higher compared to when it does not introduce SO-PMD.
- Full Text:
- Date Issued: 2009
- Authors: Musara, Vitalis
- Date: 2009
- Subjects: Optical communications , Fiber optics , Polarization (Light)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10519 , http://hdl.handle.net/10948/1138 , Optical communications , Fiber optics , Polarization (Light)
- Description: In this study, focus is centred on the measurement and emulation of first-order (FO-) and second-order (SO-) polarization mode dispersion (PMD). PMD has deleterious effects on the performance of high speed optical transmission network systems from 10 Gb/s and above. The first step was characterising deployed fibres for PMD and monitoring the state of polarization (SOP) light experiences as it propagates through the fibre. The PMD and SOP changes in deployed fibres were stochastic due to varying intrinsic and extrinsic perturbation changes. To fully understand the PMD phenomenon in terms of measurement accuracy, its complex behaviour, its implications, mitigation and compensation, PMD emulation is crucial. This thesis presents emulator designs which fall into different emulator categories. The key to these designs were the PMD equations and background on the PMD phenomenon. The cross product from the concatenation equation was applied in order to determine the coupling angle β (between 0o and 180o) that results in the SO-PMD of the emulator designs to be either adjustable or fixed. The digital delay line (DDL) or single polarization maintaining fibre (PMF) section was used to give a certain amount of FO-PMD but negligible SO-PMD. PMF sections (birefringent sections) were concatenated together to ensure FO- and SO-PMD coexist, emulating deployed fibres. FO- and SO-PMD can be controlled by altering mode coupling (coupling angles) and birefringence distribution. Emulators with PMD statistics approaching the theoretical distributions had high random coupling and several numbers of randomly distributed PMF sections. In addition, the lengths of their PMF sections lie within 20% standard deviation of the mean emulator length. Those emulators with PMD statistics that did not approach the theoretical distributions had limited numbers of randomly distributed PMF sections and mode coupling. Results also show that even when an emulator has high random mode coupling and several numbers of randomly distributed PMFs, its PMD statistics deviates away from expected theoretical distributions in the presence of polarization dependent loss (PDL). The emulators showed that the background autocorrelation function (BACF) approaches zero with increasing number of randomly mode coupled fibre sections. A zero BACF signifies that an emulator has large numbers of randomly distributed PMF sections and its presence means the opposite. The availability of SO-PMD in the emulators made the autocorrelation function (ACF) x asymmetric. In the absence of SO-PMD the ACF for a PMD emulator is symmetric. SO-PMD has no effect on the BACF. Polarization-optical time domain reflectometry (P-OTDR) measurements have shown that certain fibre sections along fibre link lengths have higher FO-PMD (HiFO-PMD) than other sections. This study investigates the impact of a HiFO-PMD section on the overall FO- and SO-PMD, the output state of polarization (SOP) and system performance on deployed fibres (through emulation). Results show that when the wavelength-independent FO-PMD vector of the HiFO-PMD section is greater than the FO-PMD contributions from the rest of the fibre link, the mean FO-PMD of the entire link is biased towards that of the HiFO-PMD section and the SO-PMD increases (β ≠ 0o or 180o) or remains fixed (β = 0o or 180o) depending on the coupling angle β between the HiFO-PMD section and the rest of the fibre link. In addition, the FO-PMD statistics deviates away from the theoretical Maxwellian distribution. However, experimental results show that the HiFO-PMD section has negligible influence on the SOPMD statistical distribution. An increase in the amount of FO-PMD on a HiFO-PMD section reduces the output SOP spread to a given minimum, in this study the minimum was reached when the HiFO-PMD ≥ 35 ps. However, the outcome of the output SOP spread depends on the location of the HiFO-PMD section along the fibre link length. It was found that when the HiFO-PMD section introduces SO-PMD, the bit error rate (BER) is much higher compared to when it does not introduce SO-PMD.
- Full Text:
- Date Issued: 2009
Racism and law : implementing the right to equality in selected South African equality courts
- Authors: Krüger, Rósaan
- Date: 2009
- Subjects: South Africa Promotion of Equality and Prevention of Unfair Discrimination Act, 2000 , Equality -- South Africa , Discrimination -- Law and legislation -- South Africa , Racism -- Law and legislation -- South Africa , Apartheid -- Law and legislation -- South Africa , Constitutional law -- South Africa , South Africa -- Politics and government -- 1994-
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3677 , http://hdl.handle.net/10962/d1003192 , South Africa Promotion of Equality and Prevention of Unfair Discrimination Act, 2000 , Equality -- South Africa , Discrimination -- Law and legislation -- South Africa , Racism -- Law and legislation -- South Africa , Apartheid -- Law and legislation -- South Africa , Constitutional law -- South Africa , South Africa -- Politics and government -- 1994-
- Description: Racism has informed South African society since colonial times. Racist beliefs found expression in the laws of colonial and apartheid South Africa and shaped both state and society. The constitutional state that South Africa has become since 1994, is based on the values of ‘human dignity’, ‘the achievement of equality’ and ‘nonracialism’, among others. Law formed the basis of the racist state prior to 1994, and now law has a fundamental role to play in the transformation of the state and society in an egalitarian direction by addressing socio-economic inequalities on the one hand, and by changing patterns of behaviour based on racist beliefs forged in the past, on the other. This thesis examines one of the legal instruments that is intended to contribute to transformation in the latter sense, namely the Promotion of Equality and Prevention of Unfair Discrimination Act 4 of 2000 (the Equality Act), with specific reference to the issue of racism. The provisions of this Act and the framework for its operation against the background of South Africa’s racist past, and within the broader framework of international and constitutional law, are examined. These two legal frameworks are analysed for the purpose of determining the standards set by international and constitutional law regarding racial equality in order to determine whether the Equality Act measures up. This thesis also incorporates an analysis of the practical application of the provisions of the Equality Act to complaints of racism in selected equality courts. The theoretical analysis of the Act’s provisions and their application in the equality courts point to various problematic formulations and obstacles which negatively affect the application of the provisions and thus hamper social change. The thesis concludes with recommendations for refining the Act’s provisions and its application.
- Full Text:
- Date Issued: 2009
- Authors: Krüger, Rósaan
- Date: 2009
- Subjects: South Africa Promotion of Equality and Prevention of Unfair Discrimination Act, 2000 , Equality -- South Africa , Discrimination -- Law and legislation -- South Africa , Racism -- Law and legislation -- South Africa , Apartheid -- Law and legislation -- South Africa , Constitutional law -- South Africa , South Africa -- Politics and government -- 1994-
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3677 , http://hdl.handle.net/10962/d1003192 , South Africa Promotion of Equality and Prevention of Unfair Discrimination Act, 2000 , Equality -- South Africa , Discrimination -- Law and legislation -- South Africa , Racism -- Law and legislation -- South Africa , Apartheid -- Law and legislation -- South Africa , Constitutional law -- South Africa , South Africa -- Politics and government -- 1994-
- Description: Racism has informed South African society since colonial times. Racist beliefs found expression in the laws of colonial and apartheid South Africa and shaped both state and society. The constitutional state that South Africa has become since 1994, is based on the values of ‘human dignity’, ‘the achievement of equality’ and ‘nonracialism’, among others. Law formed the basis of the racist state prior to 1994, and now law has a fundamental role to play in the transformation of the state and society in an egalitarian direction by addressing socio-economic inequalities on the one hand, and by changing patterns of behaviour based on racist beliefs forged in the past, on the other. This thesis examines one of the legal instruments that is intended to contribute to transformation in the latter sense, namely the Promotion of Equality and Prevention of Unfair Discrimination Act 4 of 2000 (the Equality Act), with specific reference to the issue of racism. The provisions of this Act and the framework for its operation against the background of South Africa’s racist past, and within the broader framework of international and constitutional law, are examined. These two legal frameworks are analysed for the purpose of determining the standards set by international and constitutional law regarding racial equality in order to determine whether the Equality Act measures up. This thesis also incorporates an analysis of the practical application of the provisions of the Equality Act to complaints of racism in selected equality courts. The theoretical analysis of the Act’s provisions and their application in the equality courts point to various problematic formulations and obstacles which negatively affect the application of the provisions and thus hamper social change. The thesis concludes with recommendations for refining the Act’s provisions and its application.
- Full Text:
- Date Issued: 2009
Regulating franchise operations in South Africa : a study of the existing legal framework with suggestions for reform.
- Authors: Woker, Tanya Ann
- Date: 2009
- Subjects: Franchise Association of Southern Africa , Franchises (Retail trade) -- Law and legislation -- South Africa , South Africa. Dept. of Trade and Industry , Common law -- South Africa , Commercial law -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3721 , http://hdl.handle.net/10962/d1015719
- Description: This thesis analyses the existing legal framework that applies to franchising in South Africa today. The study begins with an examination of the history and nature of the franchise contract, focusing particularly on the nature of the franchise relationship. This study is undertaken in order to substantiate the argument that franchising is a unique method of doing business. There is a need therefore to recognise that the franchise contract is a special contract in its own right, just like contracts of sale, lease, insurance and suretyship. The study then goes on to examine the problems which are experienced in the sector, as well as the law which must provide solutions to these problems. The research will show that in a modern commercial world the existing legal framework, especially the common law, cannot adequately deal with many of these problems. The complex relationship between franchising and competition law is also explored. A common thread that emerges from franchise disputes is the lack of protection afforded to the interests of franchisees. Franchisees tend to be at the mercy of economically stronger franchisors, hence the belief that there is a need for a stronger regulatory framework. The study then shifts to proposals for reform. In 2000 the Department of Trade and Industry (DTI) established the Franchise Steering Committee in conjunction with the Franchise Association of South Africa (FASA) to review the regulatory environment. This Committee drafted franchise legislation which aimed to bring the regulation of the sector under the control of the DTI. This legislation has not been implemented and the DTI has changed its strategy. Instead of dealing with franchising independently, franchising will fall within the scope of consumer protection legislation. Both the consumer protection legislation and the legislation proposed by the Franchise Steering Committee are thoroughly examined and explained. Shortcomings in the proposals are highlighted and an alternative approach is recommended. It is proposed that franchise-specific legislation should be introduced but that this legislation should establish a system of co-regulation between the government and the franchise sector.
- Full Text:
- Date Issued: 2009
- Authors: Woker, Tanya Ann
- Date: 2009
- Subjects: Franchise Association of Southern Africa , Franchises (Retail trade) -- Law and legislation -- South Africa , South Africa. Dept. of Trade and Industry , Common law -- South Africa , Commercial law -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3721 , http://hdl.handle.net/10962/d1015719
- Description: This thesis analyses the existing legal framework that applies to franchising in South Africa today. The study begins with an examination of the history and nature of the franchise contract, focusing particularly on the nature of the franchise relationship. This study is undertaken in order to substantiate the argument that franchising is a unique method of doing business. There is a need therefore to recognise that the franchise contract is a special contract in its own right, just like contracts of sale, lease, insurance and suretyship. The study then goes on to examine the problems which are experienced in the sector, as well as the law which must provide solutions to these problems. The research will show that in a modern commercial world the existing legal framework, especially the common law, cannot adequately deal with many of these problems. The complex relationship between franchising and competition law is also explored. A common thread that emerges from franchise disputes is the lack of protection afforded to the interests of franchisees. Franchisees tend to be at the mercy of economically stronger franchisors, hence the belief that there is a need for a stronger regulatory framework. The study then shifts to proposals for reform. In 2000 the Department of Trade and Industry (DTI) established the Franchise Steering Committee in conjunction with the Franchise Association of South Africa (FASA) to review the regulatory environment. This Committee drafted franchise legislation which aimed to bring the regulation of the sector under the control of the DTI. This legislation has not been implemented and the DTI has changed its strategy. Instead of dealing with franchising independently, franchising will fall within the scope of consumer protection legislation. Both the consumer protection legislation and the legislation proposed by the Franchise Steering Committee are thoroughly examined and explained. Shortcomings in the proposals are highlighted and an alternative approach is recommended. It is proposed that franchise-specific legislation should be introduced but that this legislation should establish a system of co-regulation between the government and the franchise sector.
- Full Text:
- Date Issued: 2009
Rhenium (I) and (V) complexes with potentially mulidentate ligands containing the Amino group
- Authors: Booysen, Irvin Noel
- Date: 2009
- Subjects: Rhenium , Ligands
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10386 , http://hdl.handle.net/10948/1270 , Rhenium , Ligands
- Description: The complex trans-[Re(dab)Cl3(PPh3)2] (H2dab = 1,2-diaminobenzene) was prepared from the reaction of trans-[ReOCl3(PPh3)2] with H2dab in ethanol. The ligand dab is coordinated to the rhenium(V) centre through a dianionic imido nitrogen only in a distorted octahedral coordination geometry around the metal ion. The complex trans- [Re(ada)Cl3(PPh3)2] (H2ada = 2-aminodiphenylamine) was prepared from the reaction of trans-[ReOCl3(PPh3)2] with H2ada in acetonitrile. The ligand ada is coordinated to the rhenium(V) centre through a dianionic imido nitrogen only, in a distorted octahedral coordination geometry around the metal ion. The ‘2 + 1’ complex fac- [Re(CO)3(Hamp)(amp)] (Hamp = 2-aminophenol) was isolated from the reaction of a two molar ratio of Hamp with [Re(CO)5Br] in toluene. The reaction of a 1:1 molar ratio of [Re(CO)5Br] and H2ada led to the isolation of the Re(I) complex, fac- [Re(CO)3Br(H2ada)]. The reaction of equimolar quantities of cis-[ReO2I(PPh3)2] with 5,6-diamino-1,3- dimethyluracil (H2ddd) in acetonitrile led to the formation of [Re(ddd)(Hddd)I(PPh3)2](ReO4). The X-ray crystal structure shows that the ligand ddd is coordinated monodentately through the doubly deprotonated amino nitrogen and is therefore present as an imide. The chelate Hddd is coordinated bidentately via the neutral amino nitrogen, which is coordinated trans to the imido nitrogen, and the singly deprotonated amido nitrogen, trans to the iodide. The reaction of trans-[ReOCl3(PPh3)2] with N-(2-aminobenzylidene)-5-amino-1,3-dimethyl uracil (H3dua) in ethanol gave a mixed crystal which contains both the neutral oxorhenium(V) complex [ReOCl(Hdua)] and the imido rhenium(V) [Re(dua)Cl2(PPh3)] in an equimolar ratio in the asymmetric unit. The reaction of equimolar quantities of [NH4(ReO4)] with H2ddd in methanol under reflux led to the isolation of [C12H12N6O4] as only product. The [ReO4]- anion is therefore instrumental in the formation of [C12H12N6O4], and since the product contains no rhenium in any oxidation state, the conclusion is that [ReO4]- catalyses the oxidative deamination Abstract I.N. Booysen Nelson Mandela Metropolitan University vii of H2ddd. The X-ray crystal structure consists of two centrosymmetric, tricyclic rings, comprising a central pyrazine ring and two terminal pyrimidine rings. The reaction of 2-(2-aminophenyl)benzothiazole (Habt) with [Re(CO)5Br] led to the isolation of the rhenium(I) complex fac-[Re(Habt)(CO)3Br]. With trans-[ReOCl3(PPh3)2], the ligand Habt decomposed to form the oxofree rhenium(V) complex [Re(itp)2Cl(PPh3)] (itp = 2-amidophenylthiolate). From the reaction of trans-[ReOBr3(PPh3)2] with 2-(2- hydroxyphenyl)benzothiazole (Hhpd) the complex [ReVOBr2(hpd)(PPh3)] was obtained. The reaction of a twofold molar excess of H2apb (2-(2-aminophenyl)-1-benzimidazole) with trans-[ReO2(py)4]Cl in ethanol gave the green product of formulation [ReO(Hapb)(apb)] in good yield. The rhenium atom lies in a distorted trigonalbipyramidal environment. The two imidazole N(2) atoms lie in the apical positions trans to each other, with the oxo-oxygen and two amido N(1) atoms in the trigonal plane. A new nitrosylrhenium(II) complex salt, [Re(NO)BrL2(PPh3)2](ReO4) (H2L2 = 2-amino-5- (triphenylphosphino)phenol), is the first example of a complex containing the triphenylphosphonium-amidophenolate ligand L2, formed by the nucleophilic attack of a PPh3 on a coordinated amidophenolate ring. The complex salt trans-[Re(mps)Cl(PPh3)2](ReO4) (H3mps = N-(2-amino-3- methylphenyl)salicylideneimine) was prepared by the reaction of trans-[ReOCl3(PPh3)2] with a twofold molar excess of H3mps. The X-ray crystal structure shows that the trianionic ligand mps acts as a tridentate chelate via the doubly deprotonated amino nitrogen (an imide), the neutral imino nitrogen and the deprotonated phenolic oxygen. The six-coordinated complex cis-[Re(mps)Cl2(PPh3)2] was prepared by the reaction of trans-[ReOCl3(PPh3)2] with a twofold molar excess of H3mps in benzene. The X-ray crystal structure show that the mps ligand coordinates as a tridentate chelate via the doubly deprotonated 2-amino nitrogen, the neutral imino nitrogen and the phenolate oxygen. The imide and phenolate oxygen coordinate trans to each other in a distorted octahedral geometry, around the rhenium(V) centre, with the two chlorides in cis positions. A new oxofree rhenium(V) complex salt, [Re(bbd)2](ReO4) ( H2bbd = N-(2- Abstract I.N. Booysen Nelson Mandela Metropolitan University viii aminobenzylidene)benzene-1,2-diamine), has been synthesized and the chelates bbd are coordinated as dianionic tridentate N,N,N-donor diamidoimines. The rhenium(V) ion is centered in a distorted trigonal prism. The rhenium(I) compound fac-[Re(CO)3(daa)].Hpab.H2O (Hpab = N1,N2-(1,2- phenylene)bis(2-aminobenzamide); Hdaa = 2-amino-N-(2-aminophenyl)benzamide) was synthesized from the reaction of [Re(CO)5Br] with a two equivalents of Hpab in toluene. The monoanionic tridentate ligand daa was formed by the rhenium-mediated cleavage of an amido N-C bond of the potentially tetradentate ligand Hpab. Daa is coordinated as a diaminoamide via three nitrogen-donor atoms. The reaction of a twofold molar excess of H2amben (H2amben = N1,N2-bis(2-aminobenzylidene)ethane-1,2-diamine) with trans- [ReOBr3(PPh3)2] gave the oxorhenium(V) cationic complex [ReO(amben)]X (X = Br-, PF6 -). The Re(V) oxo-bridged compound, {μ-O}[ReO(omben)]2.H2O (H2omben = N1,N2- bis(2-hydroxybenzylidene)ethane-1,2-diamine) was isolated from the reaction of a 2:1 molar ratio of H2omben and trans-[ReO2(py)4]Cl in methanol. The seven-coordinate rhenium(III) complex cation [ReIII(dhp)(PPh3)2]+ was isolated as the [ReO4]- salt from the reaction of cis-[ReVO2I(PPh3)2] with 2,6-bis(2- hydroxyphenyliminomethyl)pyridine (H2dhp) in ethanol. In the complex fac- [Re(CO)3(H2dhp)Br], prepared from [Re(CO)5Br] and H2dhp in toluene, the H2dhp ligand acts as a neutral bidentate N,N-donor chelate. An equimolar ratio reaction of 2-aminobenzaldehyde and 2-(2-aminophenyl)-1- benzimidazole in methanol led to 2-(5,6-dihydrobenzimidazolo[1,2-c]-quinazolin-6- yl)aniline. In an attempt to explore the template formation of this class of ligand with rhenium, the reaction of salicylaldehyde and 2-(2-aminophenyl)-1-benzimidazole in ethanol which was followed by the addition of trans-[ReOBr3(PPh3)2] led to the formation of the salt, 6-(2-hydroxyphenyl)-5,6-dihydrobenzimidazolo[1,2-c]quinazolin- 12-ium bromide. The compound 6-(2-methylthiophenyl)-5,6-dihydrobenzimidazolo[1,2- c]quinazolin-12-ium was synthesized via the reaction of 2-aminobenzaldehyde and 2- methylthiobenzaldehyde in methanol.
- Full Text:
- Date Issued: 2009
- Authors: Booysen, Irvin Noel
- Date: 2009
- Subjects: Rhenium , Ligands
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10386 , http://hdl.handle.net/10948/1270 , Rhenium , Ligands
- Description: The complex trans-[Re(dab)Cl3(PPh3)2] (H2dab = 1,2-diaminobenzene) was prepared from the reaction of trans-[ReOCl3(PPh3)2] with H2dab in ethanol. The ligand dab is coordinated to the rhenium(V) centre through a dianionic imido nitrogen only in a distorted octahedral coordination geometry around the metal ion. The complex trans- [Re(ada)Cl3(PPh3)2] (H2ada = 2-aminodiphenylamine) was prepared from the reaction of trans-[ReOCl3(PPh3)2] with H2ada in acetonitrile. The ligand ada is coordinated to the rhenium(V) centre through a dianionic imido nitrogen only, in a distorted octahedral coordination geometry around the metal ion. The ‘2 + 1’ complex fac- [Re(CO)3(Hamp)(amp)] (Hamp = 2-aminophenol) was isolated from the reaction of a two molar ratio of Hamp with [Re(CO)5Br] in toluene. The reaction of a 1:1 molar ratio of [Re(CO)5Br] and H2ada led to the isolation of the Re(I) complex, fac- [Re(CO)3Br(H2ada)]. The reaction of equimolar quantities of cis-[ReO2I(PPh3)2] with 5,6-diamino-1,3- dimethyluracil (H2ddd) in acetonitrile led to the formation of [Re(ddd)(Hddd)I(PPh3)2](ReO4). The X-ray crystal structure shows that the ligand ddd is coordinated monodentately through the doubly deprotonated amino nitrogen and is therefore present as an imide. The chelate Hddd is coordinated bidentately via the neutral amino nitrogen, which is coordinated trans to the imido nitrogen, and the singly deprotonated amido nitrogen, trans to the iodide. The reaction of trans-[ReOCl3(PPh3)2] with N-(2-aminobenzylidene)-5-amino-1,3-dimethyl uracil (H3dua) in ethanol gave a mixed crystal which contains both the neutral oxorhenium(V) complex [ReOCl(Hdua)] and the imido rhenium(V) [Re(dua)Cl2(PPh3)] in an equimolar ratio in the asymmetric unit. The reaction of equimolar quantities of [NH4(ReO4)] with H2ddd in methanol under reflux led to the isolation of [C12H12N6O4] as only product. The [ReO4]- anion is therefore instrumental in the formation of [C12H12N6O4], and since the product contains no rhenium in any oxidation state, the conclusion is that [ReO4]- catalyses the oxidative deamination Abstract I.N. Booysen Nelson Mandela Metropolitan University vii of H2ddd. The X-ray crystal structure consists of two centrosymmetric, tricyclic rings, comprising a central pyrazine ring and two terminal pyrimidine rings. The reaction of 2-(2-aminophenyl)benzothiazole (Habt) with [Re(CO)5Br] led to the isolation of the rhenium(I) complex fac-[Re(Habt)(CO)3Br]. With trans-[ReOCl3(PPh3)2], the ligand Habt decomposed to form the oxofree rhenium(V) complex [Re(itp)2Cl(PPh3)] (itp = 2-amidophenylthiolate). From the reaction of trans-[ReOBr3(PPh3)2] with 2-(2- hydroxyphenyl)benzothiazole (Hhpd) the complex [ReVOBr2(hpd)(PPh3)] was obtained. The reaction of a twofold molar excess of H2apb (2-(2-aminophenyl)-1-benzimidazole) with trans-[ReO2(py)4]Cl in ethanol gave the green product of formulation [ReO(Hapb)(apb)] in good yield. The rhenium atom lies in a distorted trigonalbipyramidal environment. The two imidazole N(2) atoms lie in the apical positions trans to each other, with the oxo-oxygen and two amido N(1) atoms in the trigonal plane. A new nitrosylrhenium(II) complex salt, [Re(NO)BrL2(PPh3)2](ReO4) (H2L2 = 2-amino-5- (triphenylphosphino)phenol), is the first example of a complex containing the triphenylphosphonium-amidophenolate ligand L2, formed by the nucleophilic attack of a PPh3 on a coordinated amidophenolate ring. The complex salt trans-[Re(mps)Cl(PPh3)2](ReO4) (H3mps = N-(2-amino-3- methylphenyl)salicylideneimine) was prepared by the reaction of trans-[ReOCl3(PPh3)2] with a twofold molar excess of H3mps. The X-ray crystal structure shows that the trianionic ligand mps acts as a tridentate chelate via the doubly deprotonated amino nitrogen (an imide), the neutral imino nitrogen and the deprotonated phenolic oxygen. The six-coordinated complex cis-[Re(mps)Cl2(PPh3)2] was prepared by the reaction of trans-[ReOCl3(PPh3)2] with a twofold molar excess of H3mps in benzene. The X-ray crystal structure show that the mps ligand coordinates as a tridentate chelate via the doubly deprotonated 2-amino nitrogen, the neutral imino nitrogen and the phenolate oxygen. The imide and phenolate oxygen coordinate trans to each other in a distorted octahedral geometry, around the rhenium(V) centre, with the two chlorides in cis positions. A new oxofree rhenium(V) complex salt, [Re(bbd)2](ReO4) ( H2bbd = N-(2- Abstract I.N. Booysen Nelson Mandela Metropolitan University viii aminobenzylidene)benzene-1,2-diamine), has been synthesized and the chelates bbd are coordinated as dianionic tridentate N,N,N-donor diamidoimines. The rhenium(V) ion is centered in a distorted trigonal prism. The rhenium(I) compound fac-[Re(CO)3(daa)].Hpab.H2O (Hpab = N1,N2-(1,2- phenylene)bis(2-aminobenzamide); Hdaa = 2-amino-N-(2-aminophenyl)benzamide) was synthesized from the reaction of [Re(CO)5Br] with a two equivalents of Hpab in toluene. The monoanionic tridentate ligand daa was formed by the rhenium-mediated cleavage of an amido N-C bond of the potentially tetradentate ligand Hpab. Daa is coordinated as a diaminoamide via three nitrogen-donor atoms. The reaction of a twofold molar excess of H2amben (H2amben = N1,N2-bis(2-aminobenzylidene)ethane-1,2-diamine) with trans- [ReOBr3(PPh3)2] gave the oxorhenium(V) cationic complex [ReO(amben)]X (X = Br-, PF6 -). The Re(V) oxo-bridged compound, {μ-O}[ReO(omben)]2.H2O (H2omben = N1,N2- bis(2-hydroxybenzylidene)ethane-1,2-diamine) was isolated from the reaction of a 2:1 molar ratio of H2omben and trans-[ReO2(py)4]Cl in methanol. The seven-coordinate rhenium(III) complex cation [ReIII(dhp)(PPh3)2]+ was isolated as the [ReO4]- salt from the reaction of cis-[ReVO2I(PPh3)2] with 2,6-bis(2- hydroxyphenyliminomethyl)pyridine (H2dhp) in ethanol. In the complex fac- [Re(CO)3(H2dhp)Br], prepared from [Re(CO)5Br] and H2dhp in toluene, the H2dhp ligand acts as a neutral bidentate N,N-donor chelate. An equimolar ratio reaction of 2-aminobenzaldehyde and 2-(2-aminophenyl)-1- benzimidazole in methanol led to 2-(5,6-dihydrobenzimidazolo[1,2-c]-quinazolin-6- yl)aniline. In an attempt to explore the template formation of this class of ligand with rhenium, the reaction of salicylaldehyde and 2-(2-aminophenyl)-1-benzimidazole in ethanol which was followed by the addition of trans-[ReOBr3(PPh3)2] led to the formation of the salt, 6-(2-hydroxyphenyl)-5,6-dihydrobenzimidazolo[1,2-c]quinazolin- 12-ium bromide. The compound 6-(2-methylthiophenyl)-5,6-dihydrobenzimidazolo[1,2- c]quinazolin-12-ium was synthesized via the reaction of 2-aminobenzaldehyde and 2- methylthiobenzaldehyde in methanol.
- Full Text:
- Date Issued: 2009
Social policy, welfare in urban services in South Africa : a case study of free basic water, indigency and citizenship in Eastwood, Pietermaritzburg, KwaZulu-Natal (2005-2007)
- Authors: Smith, Julie
- Date: 2009
- Subjects: Municipal water supply -- South Africa -- Pietermaritzburg , Water-supply -- Social aspects -- South Africa -- Pietermaritzburg , Poor -- South Africa -- Pietermaritzburg , Water-supply -- Economic aspects -- South Africa , South Africa -- Social policy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6060 , http://hdl.handle.net/10962/d1015231
- Description: This is an in-depth case study of urban water services to poor households and their interactions with local state power in the community of Eastwood, Pietermaritzburg, in the province of KwaZulu-Natal, South Africa, for the period 2005-2007. It draws especially on the experiences of poor women, exploring the conceptions and implications of the movement of municipal services into the realm of welfare-based urban service concessions. It interrogates what value municipal services, framed in the language and form of welfare but within a commodification milieu and in the context of shifting citizen-state relations offer the state apparatus and how such free basic service offerings are experienced by poor households at the level of domestic, social and economic functioning. The study adopts a fluid mixed-methodological approach to optimise exploration and interpretation. It argues that the interface of state service delivery and citizens is fraught with contradictions: core to this is the nature of state ' help.' Free basic water encompassed in the social wage did not improve the lives of poor households; instead it eroded original water access. Free basic water stole women's time spent on domestic activities; compromised appropriate water requirements, exacerbated service affordability problems and negatively affected household functioning. Poor households experienced the government's policy of free basic services as containment and punishment for being poor. The Indigent Policy activated the state's surveillance, disciplinary and control apparatus. In the absence of effective national regulation over municipalities and with financial shortfalls, street-level bureaucrats manipulated social policies to further municipal cost recovery goals and subjugate poor households. Social control and cheap governance were in symmetry. Citizens, desperate for relief, approached the state. Poor households were pushed into downgraded service packages or mercilessly pursued by municipally outsourced private debt collectors and disconnection companies. Municipalities competing for investments brought about by favourable credit ratings abandoned the humanity of their citizens. Such re-prioritisation of values had profound implications for governance and public trust. Citizens were jettisoned to the outskirts of municipal governance, resulting in a distinct confusion and anger towards the local state - and with it, major uncertainties regarding future stability, redistribution and equity.
- Full Text:
- Date Issued: 2009
- Authors: Smith, Julie
- Date: 2009
- Subjects: Municipal water supply -- South Africa -- Pietermaritzburg , Water-supply -- Social aspects -- South Africa -- Pietermaritzburg , Poor -- South Africa -- Pietermaritzburg , Water-supply -- Economic aspects -- South Africa , South Africa -- Social policy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6060 , http://hdl.handle.net/10962/d1015231
- Description: This is an in-depth case study of urban water services to poor households and their interactions with local state power in the community of Eastwood, Pietermaritzburg, in the province of KwaZulu-Natal, South Africa, for the period 2005-2007. It draws especially on the experiences of poor women, exploring the conceptions and implications of the movement of municipal services into the realm of welfare-based urban service concessions. It interrogates what value municipal services, framed in the language and form of welfare but within a commodification milieu and in the context of shifting citizen-state relations offer the state apparatus and how such free basic service offerings are experienced by poor households at the level of domestic, social and economic functioning. The study adopts a fluid mixed-methodological approach to optimise exploration and interpretation. It argues that the interface of state service delivery and citizens is fraught with contradictions: core to this is the nature of state ' help.' Free basic water encompassed in the social wage did not improve the lives of poor households; instead it eroded original water access. Free basic water stole women's time spent on domestic activities; compromised appropriate water requirements, exacerbated service affordability problems and negatively affected household functioning. Poor households experienced the government's policy of free basic services as containment and punishment for being poor. The Indigent Policy activated the state's surveillance, disciplinary and control apparatus. In the absence of effective national regulation over municipalities and with financial shortfalls, street-level bureaucrats manipulated social policies to further municipal cost recovery goals and subjugate poor households. Social control and cheap governance were in symmetry. Citizens, desperate for relief, approached the state. Poor households were pushed into downgraded service packages or mercilessly pursued by municipally outsourced private debt collectors and disconnection companies. Municipalities competing for investments brought about by favourable credit ratings abandoned the humanity of their citizens. Such re-prioritisation of values had profound implications for governance and public trust. Citizens were jettisoned to the outskirts of municipal governance, resulting in a distinct confusion and anger towards the local state - and with it, major uncertainties regarding future stability, redistribution and equity.
- Full Text:
- Date Issued: 2009