Influence of non-synonymous sequence mutations on the architecture of HIV-1 clade C protease receptor site : docking and molecular dynamics studies
- Authors: Onywera, David Harris
- Date: 2014
- Subjects: HIV (Viruses) -- Research , HIV infections -- Treatment -- Research , HIV infections -- Chemotherapy , Protease inhibitors -- Research , Viruses -- Effect of drugs on -- Research , Antiretroviral agents
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4116 , http://hdl.handle.net/10962/d1013133
- Description: Despite the current interventions to avert contagions and AIDS-related deaths, sub-Saharan Africa is still the region most severely affected by the HIV/AIDS pandemic, where clade C is the dominant circulating HIV-1 strain. The pol-encoded HIV-1 protease enzyme has been extensively exploited as a drug target. Protease inhibitors have been engineered within the framework of clade B, the commonest in America, Europe and Australia. Recent studies have attested the existence of sequence and catalytic disparities between clades B and C proteases that could upset drug susceptibilities. Emergence of drug-resistant associated mutations and combinatorial explosions due to recombination thwarts the attempt to stabilize the current highly active antiretroviral therapy (HAART) baseline. The project aimed at identifying the structural and molecular mechanisms hired by mutants to affect the efficacies of both FDA approved and Rhodes University (RU)-synthesized inhibitors, in order to define how current and or future drugs ought to be modified or synthesized with the intent of combating drug resistance. The rationale involved the generation of homology models of the HIV-1 sequences from the South African infants failing treatment with two protease inhibitors: lopinavir and ritonavir (as monitored by alterations in surrogate markers: CD4 cell count decline and viral load upsurge). Consistent with previous studies, we established nine polymorphisms: 12S, 15V, 19I, 36I, 41K, 63P, 69K, 89M, and 93L, linked to subtype C wild-type; some of which are associated with protease treatment in clade B. Even though we predicted two occurrence patterns of M46I, I54V and V82A mutations as V82A→I54V→M46I and I54V→V82A→M46V, other possibilities might exist. Mutations either caused a protracted or contracted active site cleft, which enforced differential drug responses. The in silico docking indicated susceptibility discordances between clades B and C in certain polymorphisms and non-polymorphisms. The RU-synthesized ligands displayed varied efficacies that were below those of the FDA approved protease inhibitors. The flaps underwent a wide range of structural motions to accommodate and stabilize the ligands. Computational analyses unravelled the need for these potential drugs to be restructured by (de novo) drug engineers to improve their binding fits, affinities, energies and interactions with multiple key protease residues in order to target resilient HIV-1 assemblages. Accumulating evidences on contrasting drug-choice interpretations from the Stanford HIVdb should act as an impetus for the customization of a HIVdb for the sub-Saharan subcontinent.
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Inside the river
- Authors: Buzani, Mangaliso Welcome
- Date: 2014
- Subjects: South African poetry (English) , Xhosa poetry
- Language: English , Xhosa
- Type: text , Thesis , Masters , MA
- Identifier: vital:5971
- Description: My collection is of imagistic associative poetry, influenced by Spanish and other kinds of modernism, and written in my two languages, isiXhosa and English. My approach to producing these poems is first via the mastery of sound, not on the actual meaning of a poem; working on the meaning would be the last stage. The language of each poem will be encountered as it comes down on the paper, leading me to the words meaningful for that particular poem.
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Inter-individual variability and phenotypic plasticity : the effect of the environment on the biogeography, population structure, ecophysiology and reproduction of the sandhoppers Talorchestia capensis and Africorchestia quadrispinosa
- Authors: Baldanzi, Simone
- Date: 2014
- Subjects: Phenotypic plasticity -- Research -- Africa, Southern Talitridae -- Research -- Africa, Southern Amphipoda -- Research -- Africa, Southern Climatic changes -- Environmental aspects -- Africa, Southern Marine biology -- Africa, Southern Adaptation (Biology) -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5846 , http://hdl.handle.net/10962/d1011447
- Description: Climatic envelope models focus on the climatic variables affecting species or species assemblages, and are important tools to investigate the effect of climate change on their geographical ranges. These models have largely been proposed in order to make successful predictions on species‘ persistence, determining which variables are likely to induce range expansion, contraction, or shifting. More recent models, including the ability and the cost for individuals to respond promptly to an environmental stimulus, have revealed that species may express phenotypic plasticity able to induce adaptation to the new environment. Consequently, understanding how species evolve to a changing climate is fundamental. From this perspective, investigating intraspecific responses to an environmental variable may contribute to better understanding and prediction of the effect of climate change on the geographical range and evolution of species, particularly in the case of widespread species. In this context, the present study aimed at establishing how environmental variables (focussing mainly on temperature) may have contributed to shape the spatial distribution, physiology, reproductive biology and connectivity of two species of Southern African sandhoppers (Talorchestia capensis and Africorchestia quadrispinosa, Amphipoda, Talitridae). Most of the work was carried out on T. capensis, due to its widespread spatial distribution. A first investigation of the biogeography of T. capensis and A. quadrispinosa, revealed that, for both species, spatial patterns of abundance, size and sex ratio were not explained by the Abundant Centre Hypothesis (greater abundance at the core of a spatial range), but rather guided by bio-physical forces. Precisely, the abundance of sandhoppers was driven by the morphodynamic state of the beach, salinity and temperatures, with strong differentiation among sites that reflected local environmental conditions. In support of these findings, strong population structure in the genetics of T. capensis was found (three main groups) when investigating its phylogeography and genetic connectivity. Although such defined structure may suggests cryptic speciation, the concomitant within-population variation in the COX1 region of mtDNA, also highlighted the importance of individual genetic variability. High individual variability was also found in the response of T. capensis to temperature, both in its physiology (thermal plasticity) and its reproductive biology (maternal effects). Since temperature is one of the main variables affecting the coastal marine systems of southern Africa and the metabolism of animals in general, its effect on the physiology and reproduction of T. capensis was therefore investigated. Thermal responses to increasing/decreasing temperatures were assessed for separated populations of T. capensis. Individual variability was reported in the oxygen consumption of T. capensis in response to temperature (high variation around the means, especially for increasing temperatures). Among population differences in thermal sensitivity were significantly correlated with air temperature variability experienced over the past 23 years, highlighting the importance of historical temperature fluctuations to the current thermal physiology of these sandhoppers. Temperature also had an important effect on the reproductive plasticity of T. capensis. Different temperatures induced mothers to adjust the size of their offspring (i.e. egg size), with larger eggs produced at lower temperatures. Interestingly, females showed strongly significant among individual variation in the size of the eggs. Given the importance of understanding rapid responses of organisms to climate change and considering the fundamental role played by phenotypic plasticity in evolution, the overall study revealed the significance of individual plasticity and variability in response to the environment and highlighted its importance. Particularly, studying the thermal physiology of separated populations and understanding within population reproductive plasticity in response to temperature, helped to clarify how differences among individual responses have important consequences at the population level, possibly explaining the widespread distribution of T. capensis.
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Intimacy, sex and sexuality : the experiences of vertically-infected HIV-positive adolescents
- Authors: Smaill, Lindsay Ann
- Date: 2014
- Subjects: Teenagers -- Sexual behavior , HIV-positive youth -- South Africa -- Eastern Cape , HIV infections -- Social aspects -- South Africa -- Eastern Cape , HIV infections -- Transmission -- South Africa -- Eastern Cape , Teenagers -- Diseases -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3225 , http://hdl.handle.net/10962/d1013026
- Description: This research explores the lived experience of being a vertically or prenatally-infected HIV-positive adolescent. It looks specifically at how the participants experience intimacy, sex and sexuality. HIV/AIDS remains a global pandemic and vertically-infected adolescents are a growing new demographic. However, there is a poverty of research, and therefore interventions and support, for this demographic. This qualitative research conducted six individual, in-depth, semi-structured, psychoanalytic research interviews with three participants. The interviews were structured around projective drawings that the participants did in the course of each interview. The interviews were transcribed and analysed using psychodynamic object relations theory and organised through interpretative phenomenological analysis. Every effort was made to ensure that the research was conducted ethically and validly. The analysis found that the participants' experience of intimacy has resulted in a self that is constantly under threat. This in turn has negatively impacted on the participants' experience of sex and sexuality. The implication of this research is that more in-depth research needs to be done into this demographic so that better interventions and support may be offered.
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Invariant optimal control on the three-dimensional semi-Euclidean group: control affine and quadratic Hamilton-Poisson systems
- Authors: Barrett, Dennis Ian
- Date: 2014
- Subjects: Automorphisms , Symmetry (Mathematics) , Lyapunov stability , Geometry, Riemannian , Geometry, Affine , Elliptic functions
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/64805 , vital:28605
- Description: In this thesis we consider invariant control systems and Hamilton-Poisson systems on the three dimensional semi-Euclidean group SE(1,1). We first classify the left-invariant control affine systems (under detached feedback equivalence). We provide a complete list of normal forms, as well as classifying conditions. As a corollary to this classification, we derive controllability criteria for control affine systems on SE(1,1). Secondly, we consider quadratic Hamilton-Poisson systems on the (minus) Lie-Poisson space se(1,1)*. These systems are classified up to an affine isomorphism. Six normal forms are identified for the homogeneous case, whereas sixteen representatives (including several infinite families) are obtained for the inhomogeneous systems. Thereafter we consider the stability and integration of the normal forms obtained. For all homogeneous systems, and a subclass of inhomogeneous systems, we perform a complete stability analysis and derive explicit expressions for all integral curves. (The extremal controls of a large class of optimal control problems on SE(1,1) are linearly related to these integral curves.) Lastly, we discuss the Riemannian and sub-Riemannian problems. The (left-invariant) Riemannian and sub-Riemannian structures on SE(1,1) are classified, up to isometric group automorphisms and scaling. Explicit expressions for the geodesics of the normalised structures are found.
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Investigating how problem solving skills can be developed using a collaborative learning environment
- Authors: Sonne, Anita
- Date: 2014
- Subjects: Mathematics -- Study and teaching (Elementary) -- South Africa , Social learning , Active learning , Problem solving in children , Educational equalization -- Research -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1977 , http://hdl.handle.net/10962/d1013017
- Description: This thesis examines whether problem solving strategies develop and improve through working in a collaborative environment and, if so, how. The study explored the way peer-topeer discussions which are focussed on finding solutions to mathematical problems might shape learners' attitudes and participation in mathematical problem solving. I use the Vygotskian (1978) socio-cultural perspective where the process of learning takes place within the Zone of Proximal Development (ZPD). Polya's problem solving heuristics (Polya, 1973) and Kilpatrick's "Instructional Triangle" (Kilpatrick, Swafford & Findell, 2001) provided the analytical framework for the study. Seven grade 7 learners from a Ex-Model C school, volunteered to participate in the study. The data gathering process involved an initial problem solving assessment, a written questionnaire, observations and video recordings of the seven learners during a series of after school problem solving sessions and post intervention learner interviews. The study showed that group discussion can have a positive impact on learners' problem solving in several respects: My key findings point to: Mathematical communication does play a role in development of problem solving strategies. A more knowledgeable other, with regards to Vygotsky's (1978) ZPD and Kilpatrick et al's (2001) instructional triangle is a critical factor in the development of problem solving strategies. All five strands of Kilpatrick et al., (2001), strands for mathematical proficiency are required for correct solutions to be calculated. At times Polya's (1973) steps for problem solving move at a rapid pace and are difficult to notice. These steps develop at different speeds for different people.
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Investigating the English vocabulary needs, exposure, and knowledge of isiXhosa speaking learners for transition from learning to read in the Foundation Phase to reading to learn in the Intermediate Phase : a case study
- Authors: Sibanda, Jabulani
- Date: 2014
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1968 , http://hdl.handle.net/10962/d1011790
- Description: The nature of learners' transition from Grade 3 to Grade 4 has serious and far reaching ramifications for their educational success in the subsequent grades. This is true of the South African education system, as it is for several other education contexts. The challenge that complicates the transition is largely a lexical one. It is with that understanding that the present study aimed to do three things namely: to determine the vocabulary needs of Grade 3 learners for transitioning to Grade 4; to establish the extent to which Grade 3 isiXhosa speaking learners are exposed to, and prepared for, the acquisition of that requisite vocabulary; and to establish the extent of Grade 4 learners' knowledge of that vocabulary at the beginning of the year. The vocabulary needs of Grade 3 learners for the transition to Grade 4 were determined from the Grade 4 subject textbook corpus. Word frequency was the criterion used to determine the usefulness and critical importance of a word for the transition. The AntConc concordance software program was used to generate word frequencies. Words with high frequency across the different subject areas, which were confirmed as high frequent in four other known word lists, were considered as constituting the vocabulary needs of learners at the verge of this significant transition. The extent of learners' preparedness for transitioning to Grade 4 in terms of their vocabulary exposure and vocabulary instruction was determined through teacher interviews, classroom observations, analysis of teacher classroom talk, analysis of the exposure and recycling of high frequent vocabulary in Grade 3 reading materials and classroom print. From these diverse sources, findings point to a paucity in both the exposure and recycling of the requisite vocabulary in these sources of classroom language input. Classroom observations and teacher interviews attest to lack of deliberate vocabulary instruction in the Grade 3 English First Additional Language lessons. The only extensive coverage of the requisite vocabulary was in the Grade 3 reading materials which included Big books, Readers and Workbooks. The Grade 4 learners' knowledge of the 60 high frequency words was tested through nine vocabulary tests, three of which tested their knowledge of word recognition, three tested passive word knowledge and the remaining three tested learners' active word knowledge. All the Grade 4 learners in the ten participating schools (297) were tested. Performance in the tests indicated that Grade 4 learners' knowledge of words requisite for reading to learn was low. That observation was consistent with an analysis of learners' performance per school, per district, per word, per test and per word bands. Tests of word recognition were done better than those of passive word knowledge and active word knowledge. Particularly problematic was test 4 which tested learners' knowledge of definition of words.
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Investigating the role of mycorrhizal fungi and associated bacteria in promoting growth of citrus seedlings
- Authors: Sitole, Phumeza
- Date: 2014
- Subjects: Mycorrhizal fungi , Citrus -- South Africa , Citrus -- Diseases and pests -- Biological control -- South Africa , Fungi as biological pest control agents , Bacteria , Phytophthora , Pythium , Indoleacetic acid
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4111 , http://hdl.handle.net/10962/d1013033
- Description: South Africa is the world's second largest exporter of fresh citrus and is ranked 14th in citrus production. Fungal pathogens such as Phytophthora and Pythium cause economic losses as a result of root rot and brown rot. Mycorrhizal fungi are specialized members of the fungal community forming a mutualistic relationship with plant roots. Mycorrhizal fungal structures are known to associate with other soil microorganisms and these may contribute to improved plant growth. A diverse group of bacteria that interact with the mycorrhizal fungi are known as Mycorrhizal Helper Bacteria (MHB). The aim of this study was to investigate the role of arbuscular mycorrhiza and associated bacteria isolated from spores and determine whether they had any plant growth promoting potential. A total of 19 bacteria were isolated from arbuscular mycorrhizal spores and were molecularly identified as belonging to several Bacillus, Micrococcus, Onchrobactrum and Staphylococcus sp. All bacterial isolates were tested for plant growth promotion abilities. One Bacillus isolate was able to solubilise phosphate. Four isolates Micrococcus sp, Micrococcus leteus, Ochrobacterum sp and Ochrobacterum antropi were able to produce Indole Acetic Acid and three isolates showed potential to reduce growth of Phytophthora nicotianae, P. citrocola and P. citrophthora in in vitro plate cultures. Further tests using culture supernatants of the Bacillus sp, Micrococcus sp and Bacillus cereus confirmed their ability to inhibit or reduce growth of the three Phytophthora species in a 96 well bioassay. Bacillus sp and Bacillus cereus were able to inhibit Phytophthora spp by 95 to 100 % and Micrococcus spp was able to decrease pathogen growth by 60 to 94 %. These bacterial isolates were further evaluated for plant growth promoting abilities on citrus rough lemon seedlings alone or in combination with arbuscular mycorrhizal inoculum. Bacterial and mycorrhizal inoculants influence the increase in shoot and root biomass. Bacillus cereus in combination with mycorrhizal inoculum significantly increased seedling shoot to root ratio while root biomass was significantly increased with mycorrhizal inoculation. Due to the short duration of the trial mycorrhizal colonisation could not be assessed. It is evident that selected combinations of bacteria and mycorrhizal fungi could promote citrus seedling growth and potentially improve seedling health. Further studies under nursery conditions are recommended.
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Is economic growth without human development sustainable? : Sub-Saharan Africa’s recent growth acceleration in context
- Authors: Hadisi Basingene, Serge
- Date: 2014
- Subjects: Sustainable development -- Africa, Sub-Saharan , Economic development -- Africa, Sub-Saharan , Poverty -- Africa, Sub-Saharan , Africa, Sub-Saharan -- Social conditions , Africa, Sub-Saharan -- Economic conditions , Africa, Sub-Saharan -- Economic policy
- Language: English
- Type: Thesis , Masters , MEcon
- Identifier: vital:1098 , http://hdl.handle.net/10962/d1013137
- Description: The purpose of the study has been to assess the question of sustainability of economic growth and human development, particularly using sub-Saharan Africa in context. Sub-Saharan Africa is an interesting case study because, on the one hand, it has been mired in poverty and remains the least developed region in the world, and on the other, it has experienced a revival in economic growth since the mid-1990s. Economists tend to use the term economic development and economic growth interchangeably. However, questions have been raised about whether Africa’s latest growth episode is indeed ‘development’. Although there are many issues at stake, the key question, and the focus of this thesis, is whether sub-Saharan Africa’s revival is sustainable. The paper sets out the debate between the ‘World Bank view’ and the ‘alternative view’. The main debate lies around how genuine development should be achieved. Firstly, the ‘World Bank view’ claims that economic growth is necessary and sufficient condition to achieve development. Economic growth will be generated by ‘orthodox’ policies and this growth will automatically trickle-down and stimulate development. Secondly, the ‘alternative view’ argues that economic growth is necessary but it is not sufficient to stimulate sustainable development. Economic growth without ‘qualitative’ change is not ‘sustainable’. Indeed, human development shortfalls (as well as other, social, political and structural problems), if not addressed through appropriate policy interventions, can undermine economic growth. The ‘alternative view’ appears to be strongly supported by evidence from other developing regions such as Latin America and East Asia. The empirical study conducted in this thesis reinforces doubts about ‘sustainability’. Even though there are signs of convergence in some indicators; this is not the case for all indicators. More importantly the gap between sub-Saharan Africa and other developing regions remains very wide. Sub-Saharan Africa’s development path remains uncertain. The intention in this study is not to be conclusive that sub-Saharan Africa cannot achieve sustainable development. Rather the study attempts to identify potential hindrances to sub-Saharan Africa’s development and to provide a solid foundation for further research in the same direction.
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Journalism sourcing and credibility: a study of Raia Mwema’s use of anonymous sources
- Authors: Mkoko, Egbert
- Date: 2014
- Subjects: Raia Mwema , Newspapers -- Tanzania , Journalism -- Tanzania , Attribution of news -- Tanzania
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3529 , http://hdl.handle.net/10962/d1013219
- Description: This study analyses how readers impute credibility to the Tanzanian investigative newspaper Raia Mwema which, as a matter of routine journalistic practice, uses a high number of anonymous sources. Against the backdrop of a strong theoretical position, espoused by media studies theorists in both Western media contexts and in Africa, in which this practice is deemed to diminish the credibility of both journalists and their stories, the study’s main purpose is to examine how readers make sense of this very prevalent practice in a country that has recently opened up to media plurality. It also investigates where they locate the source of credibility for this high-selling newspaper, if not in the traditional way through its named sources of information. The study surveys the frequency of appearance of anonymous sources in this newspaper for the period of one year from January 2011 to December 2011. Then the study considered how this practice is viewed and understood by the wider journalistic community in Tanzania as well as looking into how the journalists and owners of Raia Mwema make choices about attributing their journalism. Lastly, the study engaged with particular readers to understand what sense they make of this practice in the wider landscape of Tanzanian media and the post-repressive political situation. The study makes use of theories of the sociology of news production so as to understand the context in which Raia Mwema has routinised the practice of anonymous attribution and whether the journalistic community and newspaper readers find the practice credible. The study also employs reception analysis in order to understand to what extent Raia Mwema readers negotiate and make sense of the mainly political, and often critical, media messages they get from newspaper. In this way, it introduces the importance of the reader in the production of meaning and of assessment of credibility of journalism. The interviews – ranging from journalists working at the paper, through the wider journalistic community and taking in the readership of the paper – show that theoretical considerations of journalistic credibility must take into account the political, social and media context in which journalism is produced. Pronouncements on the overuse of anonymous sources, without understanding the way readers and journalists negotiate the complexities of an actual situation, do not tell us much about credibility and how readers understand the messages they are given. From this study, it is clear that in African countries embarking on opening media systems, credibility involves more factors than have been discussed in the literature and that readers and journalists are sophisticated consumers and producers of media messages in countries that place a host of obstacles in the way of investigative journalism and open political communication.
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Kaffirmeid and other stories
- Authors: Madingwane, June
- Date: 2014
- Subjects: South African fiction (English) -- 21st century , Short stories, South African -- 21st century , Creative writing (Higher education) -- Research -- South Africa , Creative writing -- Fiction , South African fiction (English) -- Study and teaching (Higher)
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:5983 , http://hdl.handle.net/10962/d1015659
- Description: These stories are about people’s experiences in relationships and how they overcome and resolve problems with their partners. Some explore myths and unexplained occurrences that raise many questions.
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Kedibone
- Authors: Mokae, Sabata Paul
- Date: 2014
- Subjects: Creative writing (Higher education) -- Research -- South Africa , Creative writing -- Fiction , South African fiction (English) -- Study and teaching (Higher) , South African fiction (English) -- 21st century
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6000 , http://hdl.handle.net/10962/d1020883
- Description: A young woman from a rural village near Kimberley is killed by her husband in a fit of jealousy. Her illiterate mother is summoned to the hospital to authorize the removal of vital organs – eyes, liver, kidney and heart – for organ donation. But some members of the family feel that their child should not be buried with parts of her body missing. Thus begins a story that changes the lives of many people, both black and white, over the following twenty years.
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Knowledge and knowing in the public management and public administration programmes at a comprehensive university
- Authors: Lück, Jacqueline Catherine
- Date: 2014
- Subjects: Knowledge and learning , Public administration -- Study and teaching (Higher) , Knowledge, Sociology of , Learning, Psychology of , Knowledge, Theory of , Educational equalization -- South Africa , Education, Higher -- South Africa , Literacy -- South Africa , Technical institutes -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1326 , http://hdl.handle.net/10962/d1013166
- Description: Knowledge is often tacit and under researched in educational fields. In order for student access to knowledge and its related academic discourses to be facilitated, a deep understanding needs to be gained of the form that this knowledge takes. This study interrogates the ways in which knowledge is constituted in the first year of a Public Management Diploma and a Public Administration Degree at a comprehensive university in South Africa. The study takes a social realist approach that understands reality as fact but sees our knowledge thereof as a social phenomenon. The study was concerned with knowledge structures and knower structures as it argues that these have not been adequately accounted for in the sociology of education research. But this study comes to this concern from a strongly ideological view of student reading and writing. This study calls on a social practices approach that sees literacy as embedded within specific academic discourses, which vary from context to context. It uses this ideological understanding of literacy as the orienting framework for the study of knowledge. The study takes place in a Higher Education mileu that has begun to transform from its divisive past. The transformation brought about new institutional formations such as the comprehensive university, with its mix of vocational, professional and formative programmes and varied emphasis on contextual and conceptual curriculum coherence. Increasingly, the transformation agenda also shifts concern from simply providing physical access to a previously disenfranchised majority to ensuring full participation in the context of high attrition rates in first year and low retention rates. The data was analysed using the Specialisation Codes of Legitimation Code Theory to see what was being specialised in the Diploma and Degree curricula of the Public Management and Administration fields. These fields are characterised in the literature by ongoing tensions about focus, and perceptions of there being a theoretical vacuum and an inability to deal adequately with challenges in the South African public sector. Analysis of lecturer interviews and first-year curriculum documentation showed that both the Public Management Diploma and Public Administration Degree have stronger epistemic relations (ER), with an emphasis on claims to knowledge of the world. The data showed relatively weak social relations (SR), in that there was not the valuing of a particular lens on the world or a specific disposition required for legitimation within this field. The combination of ER+ and SR- indicates that these curricula are Knowledge Codes, where legitimation is through the acquisition of a set of skills and procedures. The programmes were characterised by fairly low-level procedural knowledge, which may point to a workplace-oriented direction that is dominant in the comprehensive university. In keeping with concerns raised in the literature about this field, there was little evidence of theoretical or propositional knowledge in the Public Management Diploma and while the Public Administration Degree had some evidence of this, it was arguably not to the extent expected of a degree as described in the National Qualifications Framework. This study was limited to the first-year of the Diploma and Degree and subsequent years could present different findings. Lecturers showed awareness of student challenges with literacy practices and made concerned attempts through various interventions to address this but they were found to value the surface features of writing practices over personal engagement with the knowledge. Though the expectations of student literacy practices in tests and assignments were aligned to the ways in which knowledge was constructed in the curriculum, there was little evidence of student induction into disciplinary discourses of the field as knowledge was presented as being neutral and student writing primarily took the form of retelling objective facts. The implications of these findings could include student exclusion from higher-level academic discourse, more powerful knowledge in the workplace and, finally, constrain them from becoming producers of knowledge.
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Land redistribution and state decentralisation in South Africa
- Authors: Jaricha, Desmond Tichaona
- Date: 2014
- Subjects: Land reform -- South Africa , Land tenure -- South Africa , Agriculture -- Economic aspects -- South Africa , Decentralization in government -- South Africa , Local government -- South Africa , South Africa -- Politics and government
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3374 , http://hdl.handle.net/10962/d1013120
- Description: South Africa is a new democracy that has had to deal with many historical remnants of apartheid. One of the main remnants has been land dispossession and massive inequalities along racial lines of access to land for agricultural purposes. In countering this, the post-apartheid state has pursued land redistribution programmes since the end of apartheid in 1994, as part of a broader land reform project. Simultaneously, post-apartheid South Africa has been marked by significant state restructuring notably a process of state de-centralisation including the positioning of municipalities as development agents. Amongst other goals, this is designed to democratise the state given the authoritarian and exclusive character of the apartheid state, and thereby to democratise development initiatives and programmes. Land redistribution and state decentralisation in South Africa are different political processes with their own specific dynamics. They have though become interlinked and intertwined but not necessarily in a coherent and integrated manner. Within broader global developments pertaining to state decentralisation and land redistribution, the thesis examines the complex relations between these two processes in South Africa. In particular, I analyse critically the decentralised character of the land redistribution programme in South Africa. In order to concretise and illustrate key themes and points, I discuss a particular land redistribution project called Masizakhe located in Makana Municipality in the Eastern Cape Province.
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Learning pathways for improving rehabilitation practices in the mining industry : two cases of coal mining and borrow pits
- Authors: Mphinyane, Andani
- Date: 2014
- Subjects: Coal mines and mining -- Environmental aspects -- South Africa , Environmental education -- South Africa , Sustainable development -- South Africa , Coal miners -- Education (Continuing education) -- South Africa , Borrow pits -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1989 , http://hdl.handle.net/10962/d1013271
- Description: This research investigates cases of learning pathways for improving rehabilitation practices for key occupations in the mining industry. The study is set up as a partnership research programme between Rhodes University Environmental Learning Research Centre in South Africa, and the South African Qualification Authority, focussing on workplace learning and sustainability practices. This research programme seeks to understand the implications of the move to a knowledge society, with its emphasis on knowledge building over time, particularly in and for the environmental sector. The research was conducted as a qualitative case study that made use of semi-structured interviews, document analysis, visual photographs and observations as instruments of data gathering. Participants were sampled from two case studies, one in Limpopo province and the other one in Mpumalanga Province, who are directly involved in rehabilitation practices and related education and training programmes. The study makes use of career stories from the key occupations to provide insight into workplace learning pathways to inform education and training in the mining industry. A series of analytical statements captures some of the main findings on early education histories, career choices, learning pathway decisions and experiences related to sustainable practices and some complexities related to learning pathways. Environment and sustainability education is a cross-cutting issue in the NQF; and it pertains to the mining sector, especially to rehabilitation practices, which form the focus of this study as little is known about learning pathways associated with these sustainability practices. Insights from the study should enable the sector to enhance rehabilitation training for key occupations and at the same time encourage lifelong learning contributing towards sustainable development.
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Learning pathways of key occupations relevant to sustainable development in Makana Municipality
- Authors: Mohanoe, Elma Nthabiseng
- Date: 2014
- Subjects: Makana Municipality , Sustainable development -- South Africa -- Grahamstown , Municipal government -- South Africa -- Grahamstown , Environmental education -- South Africa -- Grahamstown , Sustainable development -- Study and teaching -- South Africa -- Grahamstown , Vocational qualifications -- South Africa -- Grahamstown , Organizational learning -- South Africa -- Grahamstown , Educational equalization -- South Africa -- Grahamstown
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1990 , http://hdl.handle.net/10962/d1013322
- Description: This study presents results to be contributed to the field of Environmental Education. It is a new arena for qualifications development and implementation in the South African Education and Training system. The study is located in the context of a joint research programme focusing on understanding issues of articulation and learning pathways development for sustainable development, established between the South African Qualifications Authority (SAQA) in partnership with Rhodes University, Environmental Learning Research Centre (ELRC). Phase 1 of the SAQA/ELRC research showed that researching workplace learning requires an understanding of learning pathways, if it is to be meaningful. It is for this reason that this research in phase 2 focuses specifically on learning pathways in the context of a local municipality in Makana. Using a case study research approach and qualitative data, this study investigated learning pathways for three occupational categories at different levels in the Makana Municipality: 1) key managerial occupations; 2) key supervisory occupations; and 3) key workers occupations relevant to sustainable development and how they are shaped and experienced. It also identified system and structural factors influencing articulation and access issues relevant to progress in learning pathways relevant to these key occupations. The study was designed using a case study research. Primarily, qualitative research techniques were employed to generate data, including observations, interviews and document analysis. The study used inductive, abductive and retroductive modes of inference to interpret and analyse data, using critical realist and systems perspectives. The findings on worker learning pathways show that there is a discrepancy between the Training Policy and the Environmental Training and Education Strategy of Makana Municipality. The issue of complexity in learning pathways and social structural factors such as inequality emerged as factors that strongly influenced learning pathways for workers. Learning pathways for workers involved in sustainable development practices hardly existed or simply did not exist. Interesting transitions associated with learning pathways such as from home, to work or no schooling in the case of the workers, showed a pattern of emergence. These showed that learning pathways are not accessible and equally available to everyone as can often erroneously be assumed. The findings on supervisor learning pathways show diverse complexities as well as related issues, when compared to the worker’s learning pathways. Issues such as overlapping of study and work emerge as influential to supervisor learning pathways. Lack of support is, however, an influencing factor, but in a different context compared to the workers, and mainly focuses on lack of bursaries, highlighting training policy issues. This aspect was found to also relate to lack of proper resources in order to enable them to learn and do their job better; an issue raised by the workers too. This challenge of lack of support in various forms posed a barrier to learning pathways. Findings related to the manager’s learning pathways show a noticeable gap between the workers, supervisors and managers. The manager’s generally have higher education qualifications related to sustainable development, and in certain cases managers have had exposure to international training related to sustainable development. Factors such as ample opportunities for learning, mentoring, association on professional bodies, and decision making powers influenced the manager’s learning pathways. It was also notable that while managers receive occupationally directed training, it is not necessarily sustainable development related. In theory, the results highlighted a need to understand systems as a whole and how their integration is important in influencing learning pathways. There were also underlying mechanisms and structures identified which needed to be unravelled and understood as these were found to influence learning pathways in this study. The study highlighted critical insights in understanding how learning pathways in a local municipality context (the case of Makana Municipality) are constructed by both systems and structural factors in the workplace, while also identifying ways in which agency of those engaged in learning for sustainable development in workplaces is enabled and /or constrained by such factors. It also showed the persistence of deep-seated inequalities of opportunity, especially for workers, to access and participate in sustainable development learning pathways.
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Lignocellulosic waste degradation using enzyme synergy with commercially available enzymes and Clostridium cellulovorans XylanaseA and MannanaseA
- Authors: Morrison, David Graham
- Date: 2014
- Subjects: Lignocellulose -- Biodegradation , Enzymes -- Biotechnology , Agricultural wastes as fuel , Polysaccharides -- Biotechnology , Sugar -- Inversion , Clostridium , Xylanases , Monomers
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4119 , http://hdl.handle.net/10962/d1013292
- Description: The launch of national and international initiatives to reduce pollution, reliance on fossil fuels and increase the beneficiation of agricultural wastes has prompted research into sugar monomer production from lignocellulosic wastes. These sugars can subsequently be used in the production of biofuels and environmentally degradable plastics. This study investigated the use of synergistic combinations of commercial and pure enzymes to lower enzyme costs and loadings, while increasing enzyme activity in the hydrolysis of agricultural waste. Pineapple pomace was selected due to its current underutilisation and the substantial quantities of it produced annually, as a by-product of pineapple canning. One of the primary costs in beneficiating agricultural wastes, such as pineapple pomace, is the high cost of enzyme solutions used to generate reducing sugars. This can be lowered through the use of synergistic combinations of enzymes. Studies related to the inclusion of hemicellulose degrading enzymes with commercial enzyme solutions have been limited and investigation of these solutions in select combinations, together with pineapple pomace substrate, allows for novel research. The use of synergistic combinations of purified cellulosomal enzymes has previously been shown to be effective at releasing reducing sugars from agricultural wastes. For the present study, MannanaseA and XylanaseA from Clostridium cellulovorans were heterologously expressed in Escherichia coli BL21 (DE3) cells and purified with immobilised metal affinity chromatography. These enzymes, in addition to two commercially available enzyme solutions (Celluclast 1.5L® and Pectinex® 3XL), were assayed on defined polysaccharides that are present in pineapple pomace to determine their substrate specificities. The degree(s) of synergy and specific activities of selected combinations of these enzymes were tested under both simultaneous and sequential conditions. It was observed that several synergistic combinations of enzyme solutions in select ratios, such as C20P60X20 (20% cellulose, 60% pectinase and 20% xylanse), C20P40X40 (20% cellulose, 40% pectinase and 40% xylanase) and C20P80 (20% cellulose, 80% pectinase) with pineapple pomace could both decrease the protein loading, while raising the level of activity compared to individual enzyme solutions. The highest quantity of reducing sugars to protein weight used on pineapple pomace was recorded at 3, 9 and 18 hours with combinations of Pectinex® 3XL and Celluclast 1.5L®, but for 27 h it was combinations of both these commercial solutions with XynA. The contribution of XynA was significant as C20P60X20 displayed the second highest reducing sugar production of 1.521 mg/mL, at 36 h from 12.875 μg/mL of protein, which was the second lowest protein loading. It was also shown that certain enzyme combinations, such as Pectinex® 3XL, Celluclast 1.5L® and XynA, did not generate synergy when combined in solution at the initial stages of hydrolysis, and instead generated a form of competition called anti-synergy. This was due to Pectinex® 3XL which had anti-synergy relationships in select combinations with the other enzyme solutions assayed. It was also observed that the degree of synergy and specific activity for a combination changed over time. Some solutions displayed the highest levels of synergy at the commencement of hydrolysis, namely Celluclast 1.5L®, ManA and XynA. Other combinations exhibited the highest levels of synergy at the end of the assay period, such as Pectinex® 3XL and Celluclast 1.5L®. Whether greater synergy was generated at the start or end of hydrolysis was a function of the stability of the enzymes in solution and whether enzyme activity increased substrate accessibility or generated competition between enzymes in solution. Sequential synergy studies demonstrated an anti-synergy relationship between Pectinex® 3XL and XynA or ManA, as well as Pectinex® 3 XL and Celluclast 1.5L®. It was found that under sequential synergy conditions with Pectinex® 3 XL, XynA and ManA, that anti-synergy could be negated and high degrees of synergy attained when the enzymes were added in specific loading orders and not inhibited by the presence of other active enzymes. The importance of loading order was demonstrated under sequential synergy conditions when XynA was added before ManA followed by Pectinex® 3 XL, which increased the activity and synergy of the solution by 50%. This equates to a 60% increase in reducing sugar release from the same concentrations of enzymes and emphasises the importance of removing anti-synergy relationships from combinations of enzymes. It can be concluded that a C20P60X20 combination (based on activity) can both synergistically increase the reducing sugar production and lower the protein loading required for pineapple pomace hydrolysis. This study also highlights the importance of reducing anti-synergy in customised enzyme cocktails and how sequential synergy can demonstrate the order in which a lignocellulosic waste is degraded.
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Linking satellite and point micrometeorological data to estimate : distributed evapotranspiration modelling based on MODIS LAI, Penman-Monteith and functional convergence theory
- Authors: Weideman, Craig Ivan
- Date: 2014
- Subjects: Plants -- Water requirements -- South Africa , Evaporation (Meteorology) -- Measurement , Satellite meteorology , Micrometeorology , Evapotranspiration , MODIS (Spectroradiometer)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4779 , http://hdl.handle.net/10962/d1012078 , Plants -- Water requirements -- South Africa , Evaporation (Meteorology) -- Measurement , Satellite meteorology , Micrometeorology , Evapotranspiration , MODIS (Spectroradiometer)
- Description: Recent advances in satellite sensor technology and micrometeorological instrumentation for water flux measurement, coupled with the expansion of automatic weather station networks that provide routine measurements of near-surface climate variables, present new opportunities for combining satellite and ground-based instrumentation to obtain distributed estimates of vegetation water use over wide areas in South Africa. In this study, a novel approach is tested, which uses satellite leaf area index (LAI) data retrieved by the Moderate Resolution Imaging Spectroradiometer (MODIS) to inform the FAO-56 Penman-Monteith equation for calculating reference evaporation (ET₀) of vegetation phenological activity. The model (ETMODIS) was validated at four sites in three different ecosystems across the country, including semi-arid savanna near Skukuza, mixed community grassland at Bellevue, near Pietermaritzburg, and Groenkop, a mixed evergreen indigenous forest near George, to determine potential for application over wider areas of the South African land surface towards meeting water resource management objectives. At Skukuza, evaluated against 170 days of flux data measured at a permanent eddy covariance (EC) flux tower in 2007, the model (ETMODIS) predicted 194.8 mm evapotranspiration relative to 148.9 mm measured fluxes, an overestimate of 31.7 %, (r² = 0.67). At an adjacent site, evaluated against flux data measured on two discrete periods of seven and eight days in February and May of 2005 using a large aperture scintillometer (SLS), ETMODIS predicted 27.4 mm and 6.7 mm evapotranspiration respectively, relative to measured fluxes of 32.5 and 8.2 mm, underestimates of 15.7 % and 18.3 % in each case (r² = 0.67 and 0.34, respectively). At Bellevue, evaluated against 235 days of evapotranspiration data measured using a surface layer scintillometer (SLS) in 2003, ETMODIS predicted 266.9 mm evapotranspiration relative to 460.2 mm measured fluxes, an underestimate of 42 % (r² = 0.67). At Groenkop, evaluated against data measured using a SLS over three discrete periods of four, seven and seven days in February, June and September/October respectively, ETMODIS predicted 9.7 mm, 10.3 mm and 17.0 mm evapotranspiration, relative to measured fluxes of 10.9 mm, 14.6 mm and 23. 9 mm, underestimates of 22.4 %, 11.2 % and 24.1 % in each case (r² = 0.98, 0.43 and 0.80, respectively). Total measured evapotranspiration exceeded total modelled evapotranspiration in all cases, with the exception of the flux tower site at Skukuza, where evapotranspiration was overestimated by ETMODIS by 31.7 % relative to measured (EC) values for the 170 days in 2007 where corresponding modelled and measured data were available. The most significant differences in measured versus predicted data were recorded at the Skukuza flux tower site in 2007 (31.7 % overestimate), and the Bellevue SLS flux site in 2003 (42 % underestimate); coefficients of determination, a measure of the extent to which modelled data are able to explain observed data at validation periods, with just two exceptions, were within a range of 0.67 – 0.98. Several sources of error and uncertainty were identified, relating predominantly to uncertainties in measured flux data used to evaluate ETMODIS, uncertainties in MODIS LAI submitted to ETMODIS, and uncertainties in ETMODIS itself, including model assumptions, and specific uncertainties relating to various inputs; further application of the model is required to test these uncertainties however, and establish confidence limits in performance. Nevertheless, the results of this study suggest that the technique is generally able to produce estimates of vegetation water use to within reasonably close approximations of measurements acquired using micrometeorological instruments, with r² values within the range of other peer-reviewed satellite remote sensing-based approaches.
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Lived bodies: women’s experience of sex and gender
- Authors: Lothian, Julie-Anne
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/69445 , vital:29538
- Description: This thesis will discuss the category of women, as a social group, through a phenomenological understanding of women’s subjective experiences. In arguing for the phenomenological perspective of the lived body, I show the ways in which other conceptions of women’s embodied subjectivity ultimately fail to provide comprehensive accounts of the lived experience of being a woman. I begin with an investigation into how biological determinists hypothesise women’s bodies as sexed. I then move to respond to Judith Butler’s poststructuralist feminist account of the gendered body. Finally, I argue that the embodied experience of being a woman is best explained as an ambiguous relationship between socially constructed expectations of femininity and biological materiality.
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Losing, using, refusing, cruising : first-generation South African women academics narrate the complexity of marginality
- Authors: Idahosa, Grace Ese-Osa
- Date: 2014
- Subjects: Women in higher education -- Research -- South Africa , Women college teachers -- Research -- South Africa , Sex discrimination in higher education -- Research -- South Africa , Feminism and higher education -- South Africa , Marginality, Social -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2880 , http://hdl.handle.net/10962/d1013148
- Description: While existing literature shows a considerable increase in the numbers of women in academia research on the experiences of women in universities has noted their continued occupation of lower status academic positions in relation to their male counterparts. As the ladder gets higher, the number of women seems to drop. These studies indicate the marginalization of women in academic settings, highlighting the various forms of subtle and overt discrimination and exclusion women face in academic work environments. In this study I ask how academic women in South Africa narrate their experience of being ‘outside in’ the teaching machine. It has been argued that intertwined sexist, patriarchal and phallocentric knowledges and practices in academic institutions produce various forms of discrimination, inequality, oppression and marginalization. Academic women report feeling invisible and retreating to the margins so as to avoid victimization and discrimination. Others have pointed to the tension between the ‘tenure clock’ and the ‘biological clock’ as a source of anxiety among academic women. Where a masculinised presentation of the self is adopted as a solution to this dilemma, the devaluation of the feminine in the academic space is confirmed. However, experiences of academic women are not identical. In the context of studies showing the importance of existing personal and social resources, prior experience and having mentors and role models in the negotiation of inequality and discrimination, I document the narratives of women academics who are the first in their families to graduate with a university degree. These first-generation academic women are therefore least likely to have access to social and cultural resources and prior experiences that can render the academic space more hospitable for the marginalised. Employing Spivak’s deconstruction of the concept of marginalisation as my primary interpretive lens, I explore the way in which, in their narratives, first-generation academic women negotiate marginality. These narratives depict a marginality that might be described, following Spivak, as ‘outside/in’, that is, as complex and involving moments of accommodation and resistance, losses and gains, pain and pride.
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