Plankton dynamics in response to algal blooms in a highly eutrophic estuary in South Africa
- Authors: Smit, Taryn
- Date: 2020
- Subjects: Algal blooms -- South Africa , Estuaries -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/49303 , vital:41619
- Description: The global rise in the incidence of harmful algal blooms in estuaries is a major concern given their importance as a nursery area for a variety of invertebrates and fishes. On the warm-temperate, south-east coast of South Africa, the Sundays Estuary experiences persistent, seasonal HABs due to stable eutrophic conditions. Spring blooms of the dinoflagellate, Heterosigma akashiwo, were recorded for several years in the estuary. This Raphidophyte is known for its consequences in fish and shellfish aquaculture and exhibits harmful properties such as mucilage and reactive oxygen species production. Subsequently, the dissertation investigated larval fish assemblage dynamics, larval prey and predator dynamics and body condition in association with H. akashiwo blooms. HABs mainly altered the water quality within the mesohaline zone in terms of microalgal biomass and dissolved oxygen. Hypereutrophic blooms of H. akashiwo reached 24 996 cells∙mL-1 during the study period. Supersaturated dissolved oxygen concentrations (up to ~13 mg∙L-1 at night and ~21 mg∙L-1 during the day) were recorded during hypereutrophic blooms (> 80 μg∙L-1, ≥ 2781 H. akashiwo cells∙mL-1). Dissolved oxygen differed significantly by bloom state, with the highest dissolved oxygen concentrations. Larval fish density, diversity and community structure variability were lower during hypereutrophic blooms compared to standard blooms and bloom absence, albeit statistically insignificant. Estuarine resident larval density was negatively correlated with dissolved oxygen concentration (P < 0.001). Dominant prey copepod of G. aestuaria larvae, P. hessei, was less abundant and variable during hypereutrophic blooms (mean: 664, range: 4-4301 individuals per m3) compared to standard blooms (mean: 950, range: 2-5237 individuals per m3) and bloom absence (mean: 1182, range: 23-7259 individuals per m3). Although HABs did not appear to drive a mismatch between prey and G. aestuaria larvae, early juvenile larvae made only a small contribution to the assemblage (0.2-1.8%). In terms of predator effects, yolk sac larvae were negatively related to R. terranatalis (P < 0.05). A significant positive relationship was found between prey, P. hessei and G. aestuaria preflexion (P < 0.001), flexion (P < 0.001) and postflexion (P < 0.05) larvae. In a world first, the effects of HABs were investigated on a larval fish species, Gilchristella aestuaria, using biochemical body condition analyses. During severe bloom conditions, G. aestuaria larvae were smaller and the assemblage consisted of mainly early developmental stages (stats). Body condition (RNA per dry weight) was poorest in the presence of hypereutrophic blooms (P < 0.001). A bell-curved relationship between RNA/DW and dissolved oxygen concentration was also produced in a 3-day time-lagged GAM (P < 0.001). In conclusion, hypereutrophic bloom conditions appeared to trigger adult G. aestuaria spawning in the upper mesohaline zone of the estuary in response to heightened primary productivity which occurs during the early bloom stage. However, reductions in copepod abundance (P. hessei) and unfavourable oxygen concentrations interfered with grow-out of G. aestuaria larvae. This research showed that HABs can alter nursery properties to the detriment of G. aestuaria larvae can have serious knock-on effects up the food chain. The loss in abundance of adult, juvenile and larval G. aestuaria, a key forage fish in South African estuaries, has major implications for estuarine food webs, especially in reduced prey availability to juvenile fishes and birds. There is still much to learn about the effects of HABs on the physiological and behavioural aspects on plankton communities worldwide.
- Full Text:
- Date Issued: 2020
- Authors: Smit, Taryn
- Date: 2020
- Subjects: Algal blooms -- South Africa , Estuaries -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/49303 , vital:41619
- Description: The global rise in the incidence of harmful algal blooms in estuaries is a major concern given their importance as a nursery area for a variety of invertebrates and fishes. On the warm-temperate, south-east coast of South Africa, the Sundays Estuary experiences persistent, seasonal HABs due to stable eutrophic conditions. Spring blooms of the dinoflagellate, Heterosigma akashiwo, were recorded for several years in the estuary. This Raphidophyte is known for its consequences in fish and shellfish aquaculture and exhibits harmful properties such as mucilage and reactive oxygen species production. Subsequently, the dissertation investigated larval fish assemblage dynamics, larval prey and predator dynamics and body condition in association with H. akashiwo blooms. HABs mainly altered the water quality within the mesohaline zone in terms of microalgal biomass and dissolved oxygen. Hypereutrophic blooms of H. akashiwo reached 24 996 cells∙mL-1 during the study period. Supersaturated dissolved oxygen concentrations (up to ~13 mg∙L-1 at night and ~21 mg∙L-1 during the day) were recorded during hypereutrophic blooms (> 80 μg∙L-1, ≥ 2781 H. akashiwo cells∙mL-1). Dissolved oxygen differed significantly by bloom state, with the highest dissolved oxygen concentrations. Larval fish density, diversity and community structure variability were lower during hypereutrophic blooms compared to standard blooms and bloom absence, albeit statistically insignificant. Estuarine resident larval density was negatively correlated with dissolved oxygen concentration (P < 0.001). Dominant prey copepod of G. aestuaria larvae, P. hessei, was less abundant and variable during hypereutrophic blooms (mean: 664, range: 4-4301 individuals per m3) compared to standard blooms (mean: 950, range: 2-5237 individuals per m3) and bloom absence (mean: 1182, range: 23-7259 individuals per m3). Although HABs did not appear to drive a mismatch between prey and G. aestuaria larvae, early juvenile larvae made only a small contribution to the assemblage (0.2-1.8%). In terms of predator effects, yolk sac larvae were negatively related to R. terranatalis (P < 0.05). A significant positive relationship was found between prey, P. hessei and G. aestuaria preflexion (P < 0.001), flexion (P < 0.001) and postflexion (P < 0.05) larvae. In a world first, the effects of HABs were investigated on a larval fish species, Gilchristella aestuaria, using biochemical body condition analyses. During severe bloom conditions, G. aestuaria larvae were smaller and the assemblage consisted of mainly early developmental stages (stats). Body condition (RNA per dry weight) was poorest in the presence of hypereutrophic blooms (P < 0.001). A bell-curved relationship between RNA/DW and dissolved oxygen concentration was also produced in a 3-day time-lagged GAM (P < 0.001). In conclusion, hypereutrophic bloom conditions appeared to trigger adult G. aestuaria spawning in the upper mesohaline zone of the estuary in response to heightened primary productivity which occurs during the early bloom stage. However, reductions in copepod abundance (P. hessei) and unfavourable oxygen concentrations interfered with grow-out of G. aestuaria larvae. This research showed that HABs can alter nursery properties to the detriment of G. aestuaria larvae can have serious knock-on effects up the food chain. The loss in abundance of adult, juvenile and larval G. aestuaria, a key forage fish in South African estuaries, has major implications for estuarine food webs, especially in reduced prey availability to juvenile fishes and birds. There is still much to learn about the effects of HABs on the physiological and behavioural aspects on plankton communities worldwide.
- Full Text:
- Date Issued: 2020
Planning considerations for smart meter implementations in South Africa
- Authors: Muchenje, Tonderai
- Date: 2020
- Subjects: Application software -- Development , Smart power grids
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/46218 , vital:39515
- Description: Smart meter implementations are still in their infancy in many African countries. This is evident by the lack of research on the subject in the African context. Most of the research studies are either Eurocentric or US-centric. Although these studies are important and informative, they might not address the African challenges in context. Hence, South Africa was chosen as the testbed for an investigation that addresses the apparent knowledge gap. This study set out to formulate a framework for planning considerations in the implementation of smart meter technology within South Africa. Through extensive literature review and analysis, the technology acceptance model (TAM) was chosen as a foundational framework for this study. Although TAM is widely used for researching technology acceptance and use, its applicability was found to be inadequate in explaining customer centric factors in smart metering. Therefore, it was supplemented with factors from the theory of reasoned action (TRA), the theory of planned behavior (TPB), privacy calculus theory (PCT), as well as the unified theory of acceptance and use of technology (UTAUT). A total of 11 consumer-centric factors were identified, and these were statistically analysed using the structural equation modelling technique (SEM). Ten (10) consumer-centric factors was found to be significant. These were attitude, perceived value, monetary cost, privacy risk, perceived ease of use, perceived usefulness, facilitating conditions, social norms, trust in technology and behavioral intention. Hypothesis testing confirmed that, not one acceptance model could adequately be used to identify and explain the consumer-centric factors that can be incorporated for planning considerations for smart meter implementation in South Africa. It was further observed that the consumer-centric factors such as environmental issues, security, reliability and health issues that were important in developed countries were not deemed so in South Africa. From a methodological perspective, the study attests to contextual localised application as opposed to universal meaning and measurement invariance when incorporating planning consideration for smart meter implementation in South Africa as compared to European countries and the United States of America. Finally, the findings hold some practical implications, as they showed the practical utility of the model in predicting the consumer-centric factors that can be incorporated for planning considerations. In support, the Business Model Canvas (BMC) was found to be a useful tool in deriving and reporting on the formulation of planning consideration guidelines. Using the BMC, five planning consideration guidelines were derived: customer segmentation, partnerships, benefits communication, value identification and customer attitude. These planning considerations will allow smart meter providers to identify their customers, partners and value propositions they might need to offer consumers to facilitate a higher smart meter acceptance and use. The proposed planning consideration guidelines can practically be used by policymakers and regulators for several aspects for future pervasive technology acceptance studies. This research has, therefore, created a platform for further research in the smart technology domain while providing a usable predictive framework for the identification of consumer-centric factors and formulation of planning considerations guidelines for smart meter implementation within the South African context.
- Full Text:
- Date Issued: 2020
- Authors: Muchenje, Tonderai
- Date: 2020
- Subjects: Application software -- Development , Smart power grids
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/46218 , vital:39515
- Description: Smart meter implementations are still in their infancy in many African countries. This is evident by the lack of research on the subject in the African context. Most of the research studies are either Eurocentric or US-centric. Although these studies are important and informative, they might not address the African challenges in context. Hence, South Africa was chosen as the testbed for an investigation that addresses the apparent knowledge gap. This study set out to formulate a framework for planning considerations in the implementation of smart meter technology within South Africa. Through extensive literature review and analysis, the technology acceptance model (TAM) was chosen as a foundational framework for this study. Although TAM is widely used for researching technology acceptance and use, its applicability was found to be inadequate in explaining customer centric factors in smart metering. Therefore, it was supplemented with factors from the theory of reasoned action (TRA), the theory of planned behavior (TPB), privacy calculus theory (PCT), as well as the unified theory of acceptance and use of technology (UTAUT). A total of 11 consumer-centric factors were identified, and these were statistically analysed using the structural equation modelling technique (SEM). Ten (10) consumer-centric factors was found to be significant. These were attitude, perceived value, monetary cost, privacy risk, perceived ease of use, perceived usefulness, facilitating conditions, social norms, trust in technology and behavioral intention. Hypothesis testing confirmed that, not one acceptance model could adequately be used to identify and explain the consumer-centric factors that can be incorporated for planning considerations for smart meter implementation in South Africa. It was further observed that the consumer-centric factors such as environmental issues, security, reliability and health issues that were important in developed countries were not deemed so in South Africa. From a methodological perspective, the study attests to contextual localised application as opposed to universal meaning and measurement invariance when incorporating planning consideration for smart meter implementation in South Africa as compared to European countries and the United States of America. Finally, the findings hold some practical implications, as they showed the practical utility of the model in predicting the consumer-centric factors that can be incorporated for planning considerations. In support, the Business Model Canvas (BMC) was found to be a useful tool in deriving and reporting on the formulation of planning consideration guidelines. Using the BMC, five planning consideration guidelines were derived: customer segmentation, partnerships, benefits communication, value identification and customer attitude. These planning considerations will allow smart meter providers to identify their customers, partners and value propositions they might need to offer consumers to facilitate a higher smart meter acceptance and use. The proposed planning consideration guidelines can practically be used by policymakers and regulators for several aspects for future pervasive technology acceptance studies. This research has, therefore, created a platform for further research in the smart technology domain while providing a usable predictive framework for the identification of consumer-centric factors and formulation of planning considerations guidelines for smart meter implementation within the South African context.
- Full Text:
- Date Issued: 2020
Promoting leader integrity through the human resource management value chain
- Authors: De Villiers, Bridget
- Date: 2020
- Subjects: Leadership -- South Africa , Leadership -- South Africa -- Moral and ethical aspects Business ethics Professional ethics Personnel management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/50469 , vital:42197
- Description: Ethical breaches in the public and private sector in South Africa are prevalent and the effects thereof pervasive on the economy and within broader society. These ethical breaches include corruption, bribery, and the mismanagement of resources and may be attributed to poor decision-making on the part of organisational leaders. The complexity of the circumstances in which organisations operate in the 21st century requires that leaders effectively navigate moral dilemmas while considering the broad range of interests represented by many organisational stakeholders. Leaders need to act in a manner that is ethically sound to avoid the negative consequences associated with unethical organisational conduct, including a loss of profit, credibility and reputation. Ethical leadership is central to moral organisational governance, as compliance with rules and regulations alone is ineffective in building and sustaining an ethical organisation. Literature points to the centrality of leader integrity to the notion of ethical leadership and that an ethical organisational culture is sustained by leaders who possess integrity. In promoting leader integrity studies highlight management interventions and organisational success factors that promote an ethical organisation. Within the context of this study these management interventions and success factors were identified as promoting leader integrity. Further, the literature identified the important role of leaders in supporting these interventions and creating the conditions necessary to foster the success factors. These management interventions are offered through and the success factors are linked to the human resource management value chain. The main aim of this study was to explore the promotion of leader integrity through the human resource management value chain in South African organisations. The main aim of the study led to the development of several theoretical and empirical sub-objectives that were addressed through the following actions: A literature study was conducted examining the nature of ethical leadership and leader integrity and included a consideration of the dark side of leadership. The literature was further examined in relation to the nature of an ethical organisation and organisational integrity, the role of the leader in promoting an ethical organisational culture, and regarding the management interventions and success factors that were seen to promote leader integrity. The insights gained from the literature review assisted in the compilation of a survey questionnaire, this being the Management Interventions and Success Factors Scale. This scale, together with an existing scale, The Perceived Leader Integrity Scale (version 1.2), were used in the empirical study to determine the perceptions of employees as to whether specific management interventions and success factors were seen to promote leader integrity, and the extent to which their managers were seen to act with integrity. These employees worked in the public and private sectors across South Africa, and a final sample size of 606 respondents was achieved. The empirical results of the study revealed that management interventions and success factors were seen to promote leader integrity. However, due to high levels of inconsistency in the responses received it was recommended that a clearer link needs to be created in terms of how the success factors and interventions promote leader integrity. The empirical study further revealed that South African leaders were seen to act with integrity, more especially not to engage in acts of extreme negative deviance such as theft or sabotage, but that there was room for improvement as there were employees who reported moderate and low levels of integrity among their managers. Together with the literature review the empirical study contributed towards the development of a model and a framework for the promotion of leader integrity across the HRM value chain. South African leaders have a crucial role to play in promoting not only the moral fibre of the organisations that they represent, but also in contributing positively to moral regeneration at both a national and a global level through effective and integrity-based organisational governance. This is achieved through adopting an integrated, strategic and holistic approach to the promotion of leader integrity as proposed in this study.
- Full Text:
- Date Issued: 2020
- Authors: De Villiers, Bridget
- Date: 2020
- Subjects: Leadership -- South Africa , Leadership -- South Africa -- Moral and ethical aspects Business ethics Professional ethics Personnel management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/50469 , vital:42197
- Description: Ethical breaches in the public and private sector in South Africa are prevalent and the effects thereof pervasive on the economy and within broader society. These ethical breaches include corruption, bribery, and the mismanagement of resources and may be attributed to poor decision-making on the part of organisational leaders. The complexity of the circumstances in which organisations operate in the 21st century requires that leaders effectively navigate moral dilemmas while considering the broad range of interests represented by many organisational stakeholders. Leaders need to act in a manner that is ethically sound to avoid the negative consequences associated with unethical organisational conduct, including a loss of profit, credibility and reputation. Ethical leadership is central to moral organisational governance, as compliance with rules and regulations alone is ineffective in building and sustaining an ethical organisation. Literature points to the centrality of leader integrity to the notion of ethical leadership and that an ethical organisational culture is sustained by leaders who possess integrity. In promoting leader integrity studies highlight management interventions and organisational success factors that promote an ethical organisation. Within the context of this study these management interventions and success factors were identified as promoting leader integrity. Further, the literature identified the important role of leaders in supporting these interventions and creating the conditions necessary to foster the success factors. These management interventions are offered through and the success factors are linked to the human resource management value chain. The main aim of this study was to explore the promotion of leader integrity through the human resource management value chain in South African organisations. The main aim of the study led to the development of several theoretical and empirical sub-objectives that were addressed through the following actions: A literature study was conducted examining the nature of ethical leadership and leader integrity and included a consideration of the dark side of leadership. The literature was further examined in relation to the nature of an ethical organisation and organisational integrity, the role of the leader in promoting an ethical organisational culture, and regarding the management interventions and success factors that were seen to promote leader integrity. The insights gained from the literature review assisted in the compilation of a survey questionnaire, this being the Management Interventions and Success Factors Scale. This scale, together with an existing scale, The Perceived Leader Integrity Scale (version 1.2), were used in the empirical study to determine the perceptions of employees as to whether specific management interventions and success factors were seen to promote leader integrity, and the extent to which their managers were seen to act with integrity. These employees worked in the public and private sectors across South Africa, and a final sample size of 606 respondents was achieved. The empirical results of the study revealed that management interventions and success factors were seen to promote leader integrity. However, due to high levels of inconsistency in the responses received it was recommended that a clearer link needs to be created in terms of how the success factors and interventions promote leader integrity. The empirical study further revealed that South African leaders were seen to act with integrity, more especially not to engage in acts of extreme negative deviance such as theft or sabotage, but that there was room for improvement as there were employees who reported moderate and low levels of integrity among their managers. Together with the literature review the empirical study contributed towards the development of a model and a framework for the promotion of leader integrity across the HRM value chain. South African leaders have a crucial role to play in promoting not only the moral fibre of the organisations that they represent, but also in contributing positively to moral regeneration at both a national and a global level through effective and integrity-based organisational governance. This is achieved through adopting an integrated, strategic and holistic approach to the promotion of leader integrity as proposed in this study.
- Full Text:
- Date Issued: 2020
Radical transformation and sustainability for an advanced manufacturing industry: a progressive model
- Noor, Richard, Van der Merwe, Karl
- Authors: Noor, Richard , Van der Merwe, Karl
- Date: 2020
- Subjects: Manufacturing industries -- South Africa , Leadership Organisational learning
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/46849 , vital:39669
- Description: The manufacturing industry in one of the most significant contributors to a countries GDP across the world. South Africa is no different and yet it remains an interesting challenge for the country. Power and greed seem to have overtaken the moral fibre of our society and has inflicted pain and suffering to millions across the country. The manufacturing has not been immune to this challenge and appears to be in free fall on so many levels. The primary objective of this research was to develop a model for the manufacturing industry which would assist the role players to navigate the challenges and obstacle in this ever changing and demanding industry. An extensive review of literature was conducted which informed and guided the process of gathering information and data using a questionnaire and a survey conducted across the manufacturing industry. Structural Equation Modelling was used to test the relationships between the various factors identified during the literature review. Leadership Impact was confirmed as a significant contributor as well as the Economic Impact having a significant impact on both Radical Transformation and Sustainability. This study scientifically added to the body of knowledge having taken several factors within the manufacturing industry and analysed its impact on radical transformation and sustainability, the integration of these factors within one study has not been previously conducted and has contributed to the manufacturing industry, business leadership and management fields of study.
- Full Text:
- Date Issued: 2020
- Authors: Noor, Richard , Van der Merwe, Karl
- Date: 2020
- Subjects: Manufacturing industries -- South Africa , Leadership Organisational learning
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/46849 , vital:39669
- Description: The manufacturing industry in one of the most significant contributors to a countries GDP across the world. South Africa is no different and yet it remains an interesting challenge for the country. Power and greed seem to have overtaken the moral fibre of our society and has inflicted pain and suffering to millions across the country. The manufacturing has not been immune to this challenge and appears to be in free fall on so many levels. The primary objective of this research was to develop a model for the manufacturing industry which would assist the role players to navigate the challenges and obstacle in this ever changing and demanding industry. An extensive review of literature was conducted which informed and guided the process of gathering information and data using a questionnaire and a survey conducted across the manufacturing industry. Structural Equation Modelling was used to test the relationships between the various factors identified during the literature review. Leadership Impact was confirmed as a significant contributor as well as the Economic Impact having a significant impact on both Radical Transformation and Sustainability. This study scientifically added to the body of knowledge having taken several factors within the manufacturing industry and analysed its impact on radical transformation and sustainability, the integration of these factors within one study has not been previously conducted and has contributed to the manufacturing industry, business leadership and management fields of study.
- Full Text:
- Date Issued: 2020
The potential of Raman spectroscopy in distinguishing between wool and mohair fibres
- Authors: Notayi, Mzwamadoda
- Date: 2020
- Subjects: Textile fabrics , Textile fibers -- Mechanical properties Wool -- Dissertations Mohair -- Dissertations
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/49248 , vital:41614
- Description: The possible application of the FT Raman, Raman micro-spectroscopy and ATR-FTIR micro-spectroscopy, have been investigated for distinguishing between wool and mohair. Highly identical Raman and FTIR spectra were obtained from the two fibre types, indicating that indeed they share similar basic molecular structural chemistry. The analysis of the amide I through curve fitting of wool and mohair FT Raman spectra showed that the protein and polypeptide secondary structure exists mainly in the α-helical structural conformation with smaller proportions of β-pleated sheet and β-Turns. These proportions, however, could not be used to distinguish between wool and mohair, due to the significant overlap observed between the two fibres. This study also determined the disulphide contents for possibly distinguishing between wool and mohair fibres, with the average and standard deviation values of 0.20±0.04 and 0.17±0.03 for wool and mohair, respectively, being found. Despite the mean values being found to differ statistically significant (p<0.05), a considerable overlap was observed, posing a doubt in the possible application of the method for distinguishing between the two fibres and blend composition analysis of the two fibres. The application of ratiometric analysis, based on the relative peak heights of certain FT Raman bands, showed that a combination of ratios A (I2932/I1450) and D (I508/I1450) could hold great potential in distinguishing between wool and mohair fibre samples. The individual values of ratios A and D varied a great deal from one mohair sample to the other and even more from one wool sample to another, with the individual values for ratio A ranging from 2.71-3.68 and 2.35-3.08 for wool and mohair, respectively, while ratio D ranged from 0.18-0.32 and 0.17-0.22 for wool and mohair, respectively. An important observation from this study is that if, for an unknown sample, if individual values of ratios A and D exceed 3.1 and 0.22, respectively, are found then the sample is most likely to be either a pure wool or blend of wool and mohair, whereas if all the values fall below the two threshold values, then the unknown sample can be declared a pure mohair sample. A Raman spectral database or library of approximately 100 high quality Raman average spectra of wool and mohair fibres has been established for the Bruker 80V FTIR/Raman spectrophotometer at the Nelson Mandela University (NMU). Although this has not been fully validated due to the unforeseen frequent breakdown encountered with the FT Raman system, at this stage, it has been realized that verification of unknown materials is highly possible. A great need for the development of a classification model based on multivariate or chemometrics has been realized. An ATR-FTIR LUMOS micro-spectroscopic system was also investigated for the possible application in distinguishing between wool and mohair single fibres. The amide I/II band ratios were determined for both wool and mohair fibres to distinguish between the two fibre types. The mean and standard deviation values of 1.20±0.02 and 1.21±0.01 for mohair and wool, respectively, were found and were shown not to differ statistically significant (p˃0.05). The secondary structure analysis showed that the content of the α-helical secondary structure might be different between the two fibre types, with a great overlap of individual values, however, being observed between the two fibre types (wool and mohair), raising concerns in the possible application of the α-helical content for distinguishing the two fibres.
- Full Text:
- Date Issued: 2020
- Authors: Notayi, Mzwamadoda
- Date: 2020
- Subjects: Textile fabrics , Textile fibers -- Mechanical properties Wool -- Dissertations Mohair -- Dissertations
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/49248 , vital:41614
- Description: The possible application of the FT Raman, Raman micro-spectroscopy and ATR-FTIR micro-spectroscopy, have been investigated for distinguishing between wool and mohair. Highly identical Raman and FTIR spectra were obtained from the two fibre types, indicating that indeed they share similar basic molecular structural chemistry. The analysis of the amide I through curve fitting of wool and mohair FT Raman spectra showed that the protein and polypeptide secondary structure exists mainly in the α-helical structural conformation with smaller proportions of β-pleated sheet and β-Turns. These proportions, however, could not be used to distinguish between wool and mohair, due to the significant overlap observed between the two fibres. This study also determined the disulphide contents for possibly distinguishing between wool and mohair fibres, with the average and standard deviation values of 0.20±0.04 and 0.17±0.03 for wool and mohair, respectively, being found. Despite the mean values being found to differ statistically significant (p<0.05), a considerable overlap was observed, posing a doubt in the possible application of the method for distinguishing between the two fibres and blend composition analysis of the two fibres. The application of ratiometric analysis, based on the relative peak heights of certain FT Raman bands, showed that a combination of ratios A (I2932/I1450) and D (I508/I1450) could hold great potential in distinguishing between wool and mohair fibre samples. The individual values of ratios A and D varied a great deal from one mohair sample to the other and even more from one wool sample to another, with the individual values for ratio A ranging from 2.71-3.68 and 2.35-3.08 for wool and mohair, respectively, while ratio D ranged from 0.18-0.32 and 0.17-0.22 for wool and mohair, respectively. An important observation from this study is that if, for an unknown sample, if individual values of ratios A and D exceed 3.1 and 0.22, respectively, are found then the sample is most likely to be either a pure wool or blend of wool and mohair, whereas if all the values fall below the two threshold values, then the unknown sample can be declared a pure mohair sample. A Raman spectral database or library of approximately 100 high quality Raman average spectra of wool and mohair fibres has been established for the Bruker 80V FTIR/Raman spectrophotometer at the Nelson Mandela University (NMU). Although this has not been fully validated due to the unforeseen frequent breakdown encountered with the FT Raman system, at this stage, it has been realized that verification of unknown materials is highly possible. A great need for the development of a classification model based on multivariate or chemometrics has been realized. An ATR-FTIR LUMOS micro-spectroscopic system was also investigated for the possible application in distinguishing between wool and mohair single fibres. The amide I/II band ratios were determined for both wool and mohair fibres to distinguish between the two fibre types. The mean and standard deviation values of 1.20±0.02 and 1.21±0.01 for mohair and wool, respectively, were found and were shown not to differ statistically significant (p˃0.05). The secondary structure analysis showed that the content of the α-helical secondary structure might be different between the two fibre types, with a great overlap of individual values, however, being observed between the two fibre types (wool and mohair), raising concerns in the possible application of the α-helical content for distinguishing the two fibres.
- Full Text:
- Date Issued: 2020
Water balance processes in indigenous and introduced spaces three production system in the Southern Cape region of South Africa
- Authors: Mapeto, Tatenda
- Date: 2020
- Subjects: Hydrology -- Research -- South Africa , Groundwater -- Research -- South Africa Trees -- Breeding Sustainable forestry
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/49325 , vital:41621
- Description: Forests and trees are important modulators of water flow and their position in the landscape impacts the spatial and temporal availability of ground water and surface water resources. As such, the global extent of natural forests is linked to the availability of water. In the circumstances where tree production systems have been introduced and are managed in landscapes previously populated with other forms of vegetation, it is necessary to understand their hydrological impact so as to ensure that the benefits derived from these tree systems do not outweigh the costs of water availability for sustaining human and environmental water requirements.
- Full Text: false
- Date Issued: 2020
- Authors: Mapeto, Tatenda
- Date: 2020
- Subjects: Hydrology -- Research -- South Africa , Groundwater -- Research -- South Africa Trees -- Breeding Sustainable forestry
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/49325 , vital:41621
- Description: Forests and trees are important modulators of water flow and their position in the landscape impacts the spatial and temporal availability of ground water and surface water resources. As such, the global extent of natural forests is linked to the availability of water. In the circumstances where tree production systems have been introduced and are managed in landscapes previously populated with other forms of vegetation, it is necessary to understand their hydrological impact so as to ensure that the benefits derived from these tree systems do not outweigh the costs of water availability for sustaining human and environmental water requirements.
- Full Text: false
- Date Issued: 2020