The hydrogeomorphology of the Featherstone Kloof Catchment
- Authors: Ntakumba, Stanley Sixolile
- Date: 2013-05-29
- Subjects: Wetlands -- South Africa , Wetland restoration -- South Africa -- Featherstone Kloof Catchment , Wetland conservation -- South Africa -- Featherstone Kloof Catchment , Wetland management -- South Africa -- Featherstone Kloof Catchment , Wetland ecology -- South Africa -- Featherstone Kloof Catchment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4870 , http://hdl.handle.net/10962/d1007862 , Wetlands -- South Africa , Wetland restoration -- South Africa -- Featherstone Kloof Catchment , Wetland conservation -- South Africa -- Featherstone Kloof Catchment , Wetland management -- South Africa -- Featherstone Kloof Catchment , Wetland ecology -- South Africa -- Featherstone Kloof Catchment
- Description: Wetlands are an important part of the landscape as hydrogeomorphological ecosystems. Over the centuries their importance has not received relevant attention; instead they have been treated as wastelands impeding development for maximum economic benefits. Research evidence from different parts of the world has influenced the change of such negative perceptions to an extent that the issue of wetlands' rehabilitation/restoration, conservation and management is firmly on the global agenda and local agendas of various countries, as evidenced by the adoption of the Ramsar Convention in 1971, and the Working for Water and Working for Wetlands programmes of the South African government. The aim of this research was to investigate the hydrological and geomorphological functions of a headwater wetland located in the Featherstone Kloof Catchment near Grahamstown, South Africa. The research was based on the hypotheses that wetlands store sediments, attenuate floods, store water and prolong downstream flows. A literature survey was conducted to gauge the state of knowledge about wetlands, particularly their hydrogeomorphology. An attempt was made to locate the study area within the broad historical and spatial context using a number of methods, including the radiocarbon dating of wetland sediments, the review of relevant literature and the analysis of historical hydroclimatic data. The results revealed that the wetland has existed for approximately 2000 years - as the oldest radiocarbon date obtained was 1850±50 BP. An analysis of more than a century (+120 years) long Grahamstown rainfall series indicated a steady fluctuation of rainfall around the mean, with regular decada1 cycles of wet and dry spells. Years with more rain below average were more common than those with higher rainfall, and storms events were quite common in the III area over the period. The distribution of seasons in the area over a calendar year period was demonstrated through the use of evaporation data. An intensive monitoring of hydrological and geomorphological variables was carried out using a combination of methods. The topography of the instrumented site was determined using a Total Station from reference benchmarks. Hydrological measurements included a nest of forty-eight piezometers for water table monitoring, and streamflow gauges at the upstream and downstream limits of the study site. Soil stratigraphic analysis was carried out through field techniques and laboratory measurements. A survey of wetland sediments was carried out after the main floods events. Data generated were used to analyse relationships between various variables and their role on the functioning of the wetland. The water balance of the wetland was quantified. The results indicated that the wetland was able to perform the cited hydrogeomorphological functions to some extent. For example, one of the key findings of this research is that the wetland was important in sustaining base flows under normal circumstances. However, the wetland did little to attenuate large floods. The results also revealed some important questions that require further research, including the role played by extreme flood events in altering wetland characteristics, the contribution of each water balance component in the hydrological functioning of wetlands, and importance of quantifying sediment budgets of headwater wetlands. The study demonstrated the complex nature of the wetland hydro geomorphology and that certain questions about wetlands require direct field monitoring to be better understood. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Ntakumba, Stanley Sixolile
- Date: 2013-05-29
- Subjects: Wetlands -- South Africa , Wetland restoration -- South Africa -- Featherstone Kloof Catchment , Wetland conservation -- South Africa -- Featherstone Kloof Catchment , Wetland management -- South Africa -- Featherstone Kloof Catchment , Wetland ecology -- South Africa -- Featherstone Kloof Catchment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4870 , http://hdl.handle.net/10962/d1007862 , Wetlands -- South Africa , Wetland restoration -- South Africa -- Featherstone Kloof Catchment , Wetland conservation -- South Africa -- Featherstone Kloof Catchment , Wetland management -- South Africa -- Featherstone Kloof Catchment , Wetland ecology -- South Africa -- Featherstone Kloof Catchment
- Description: Wetlands are an important part of the landscape as hydrogeomorphological ecosystems. Over the centuries their importance has not received relevant attention; instead they have been treated as wastelands impeding development for maximum economic benefits. Research evidence from different parts of the world has influenced the change of such negative perceptions to an extent that the issue of wetlands' rehabilitation/restoration, conservation and management is firmly on the global agenda and local agendas of various countries, as evidenced by the adoption of the Ramsar Convention in 1971, and the Working for Water and Working for Wetlands programmes of the South African government. The aim of this research was to investigate the hydrological and geomorphological functions of a headwater wetland located in the Featherstone Kloof Catchment near Grahamstown, South Africa. The research was based on the hypotheses that wetlands store sediments, attenuate floods, store water and prolong downstream flows. A literature survey was conducted to gauge the state of knowledge about wetlands, particularly their hydrogeomorphology. An attempt was made to locate the study area within the broad historical and spatial context using a number of methods, including the radiocarbon dating of wetland sediments, the review of relevant literature and the analysis of historical hydroclimatic data. The results revealed that the wetland has existed for approximately 2000 years - as the oldest radiocarbon date obtained was 1850±50 BP. An analysis of more than a century (+120 years) long Grahamstown rainfall series indicated a steady fluctuation of rainfall around the mean, with regular decada1 cycles of wet and dry spells. Years with more rain below average were more common than those with higher rainfall, and storms events were quite common in the III area over the period. The distribution of seasons in the area over a calendar year period was demonstrated through the use of evaporation data. An intensive monitoring of hydrological and geomorphological variables was carried out using a combination of methods. The topography of the instrumented site was determined using a Total Station from reference benchmarks. Hydrological measurements included a nest of forty-eight piezometers for water table monitoring, and streamflow gauges at the upstream and downstream limits of the study site. Soil stratigraphic analysis was carried out through field techniques and laboratory measurements. A survey of wetland sediments was carried out after the main floods events. Data generated were used to analyse relationships between various variables and their role on the functioning of the wetland. The water balance of the wetland was quantified. The results indicated that the wetland was able to perform the cited hydrogeomorphological functions to some extent. For example, one of the key findings of this research is that the wetland was important in sustaining base flows under normal circumstances. However, the wetland did little to attenuate large floods. The results also revealed some important questions that require further research, including the role played by extreme flood events in altering wetland characteristics, the contribution of each water balance component in the hydrological functioning of wetlands, and importance of quantifying sediment budgets of headwater wetlands. The study demonstrated the complex nature of the wetland hydro geomorphology and that certain questions about wetlands require direct field monitoring to be better understood. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
Synthesis and evaluation of PGM-selective ligands
- Gxoyiya, Babalwa Siliziwe Blossom
- Authors: Gxoyiya, Babalwa Siliziwe Blossom
- Date: 2013-05-28
- Subjects: Platinum group , Ligands , Ligands -- Evaluation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4450 , http://hdl.handle.net/10962/d1007849 , Platinum group , Ligands , Ligands -- Evaluation
- Description: A series of polydentate POM-selective, sulfur-containing amide ligands have been synthesized from ro-dibromoalkanes and mercaptoacetanilide, The resulting 3,6- dithiaoctanediamides and 3,7-dithianonanediamides, some of which contain a polymerisable group, were all characterized by high-resolution MS, IR, I Hand I3C NMR spectroscopic methods. Various approaches to the polymerisable ligands were explored, the most efficient proving to be the incorporation of an allyl ether moiety in the mercaptoacetanilide. The corresponding Pd(U) and Pt(II) complexes were also prepared from the metal chloride salts and characterized by elemental analysis and spectroscopic methods. The NMR data indicates that both the cis- and transcomplexes were formed, while the IR data indicates cis- coordination of the chlorine . ligands. Molecularly imprinted polymers (MIP's), prepared using platinum(II) mercaptoacetanilide and 3,6-dithiadiamide complexes, showed high selectivity for , , palladium(II) [in the presence of Pt(II), CoCII), Cu(II) and Ni(II)] as determined by . ICP-MS analysis. The more kinetically inert Pt(II) ions however, slowly displaced Pd(II), confirming the Pt(II) selectivity of the MIP's. Solvent extraction studies were conducted to explore the selectivity of the 3,6- dithiaoctanediamides and 3,7-dithianonanediamides for Pd(U) over CoCII), Cu(U) and Ni(II). The ICP-MS data indicate that, in general, equilibration was achieved within ten minutes and that the longer-chain amides were less selective than the shorter-chain analogues. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Gxoyiya, Babalwa Siliziwe Blossom
- Date: 2013-05-28
- Subjects: Platinum group , Ligands , Ligands -- Evaluation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4450 , http://hdl.handle.net/10962/d1007849 , Platinum group , Ligands , Ligands -- Evaluation
- Description: A series of polydentate POM-selective, sulfur-containing amide ligands have been synthesized from ro-dibromoalkanes and mercaptoacetanilide, The resulting 3,6- dithiaoctanediamides and 3,7-dithianonanediamides, some of which contain a polymerisable group, were all characterized by high-resolution MS, IR, I Hand I3C NMR spectroscopic methods. Various approaches to the polymerisable ligands were explored, the most efficient proving to be the incorporation of an allyl ether moiety in the mercaptoacetanilide. The corresponding Pd(U) and Pt(II) complexes were also prepared from the metal chloride salts and characterized by elemental analysis and spectroscopic methods. The NMR data indicates that both the cis- and transcomplexes were formed, while the IR data indicates cis- coordination of the chlorine . ligands. Molecularly imprinted polymers (MIP's), prepared using platinum(II) mercaptoacetanilide and 3,6-dithiadiamide complexes, showed high selectivity for , , palladium(II) [in the presence of Pt(II), CoCII), Cu(II) and Ni(II)] as determined by . ICP-MS analysis. The more kinetically inert Pt(II) ions however, slowly displaced Pd(II), confirming the Pt(II) selectivity of the MIP's. Solvent extraction studies were conducted to explore the selectivity of the 3,6- dithiaoctanediamides and 3,7-dithianonanediamides for Pd(U) over CoCII), Cu(U) and Ni(II). The ICP-MS data indicate that, in general, equilibration was achieved within ten minutes and that the longer-chain amides were less selective than the shorter-chain analogues. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
A comparative study of the Linux and windows device driver architecture with a focus on IEEE1394 (high speed serial bus) drivers
- Authors: Tsegaye, Melekam Asrat
- Date: 2004
- Subjects: Microsoft Windows (Computer file) , Linux , Operating systems (Computers) , DOS device drivers (Computer programs) , Linux device drivers (Computer programs)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4591 , http://hdl.handle.net/10962/d1004829 , Microsoft Windows (Computer file) , Linux , Operating systems (Computers) , DOS device drivers (Computer programs) , Linux device drivers (Computer programs)
- Description: New hardware devices are continually being released to the public by hardware manufactures around the world. For these new devices to be usable under a PC operating system, device drivers that extend the functionality of the target operating system have to be constructed. This work examines and compares the device driver architectures currently in use by two of the most widely used operating systems, Microsoft’s Windows and Linux. The IEEE1394 (high speed serial bus) device driver stacks on each operating system are examined and compared as an example of a major device driver stack implementation, including driver requirements for the upcoming IEEE1394.1 bridging standard.
- Full Text:
- Date Issued: 2004
- Authors: Tsegaye, Melekam Asrat
- Date: 2004
- Subjects: Microsoft Windows (Computer file) , Linux , Operating systems (Computers) , DOS device drivers (Computer programs) , Linux device drivers (Computer programs)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4591 , http://hdl.handle.net/10962/d1004829 , Microsoft Windows (Computer file) , Linux , Operating systems (Computers) , DOS device drivers (Computer programs) , Linux device drivers (Computer programs)
- Description: New hardware devices are continually being released to the public by hardware manufactures around the world. For these new devices to be usable under a PC operating system, device drivers that extend the functionality of the target operating system have to be constructed. This work examines and compares the device driver architectures currently in use by two of the most widely used operating systems, Microsoft’s Windows and Linux. The IEEE1394 (high speed serial bus) device driver stacks on each operating system are examined and compared as an example of a major device driver stack implementation, including driver requirements for the upcoming IEEE1394.1 bridging standard.
- Full Text:
- Date Issued: 2004
A comparison of web-based technologies to serve images from an Oracle9i database
- Authors: Swales, Dylan
- Date: 2004 , 2013-06-18
- Subjects: Active server pages , Microsoft .NET , JavaServer pages , Oracle (Computer file) , Internet searching , Web site development--Computer programs , World Wide Web , Online information services
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4583 , http://hdl.handle.net/10962/d1004380 , Active server pages , Microsoft .NET , JavaServer pages , Oracle (Computer file) , Internet searching , Web site development--Computer programs , World Wide Web , Online information services
- Description: The nature of Internet and Intranet Web applications has changed from a static content-distribution medium into an interactive, dynamic medium, often used to serve multimedia from back-end object-relational databases to Web-enabled clients. Consequently, developers need to make an informed technological choice for developing software that supports a Web-based application for distributing multimedia over networks. This decision is based on several factors. Among the factors are ease of programming, richness of features, scalability, and performance. The research focuses on these key factors when distributing images from an Oracle9i database using Java Servlets, JSP, ASP, and ASP.NET as the server-side development technologies. Prototype applications are developed and tested within each technology: one for single image serving and the other for multiple image serving. A matrix of recommendations is provided to distinguish which technology, or combination of technologies, provides the best performance and development platform for image serving within the studied envirorunent. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2004
- Authors: Swales, Dylan
- Date: 2004 , 2013-06-18
- Subjects: Active server pages , Microsoft .NET , JavaServer pages , Oracle (Computer file) , Internet searching , Web site development--Computer programs , World Wide Web , Online information services
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4583 , http://hdl.handle.net/10962/d1004380 , Active server pages , Microsoft .NET , JavaServer pages , Oracle (Computer file) , Internet searching , Web site development--Computer programs , World Wide Web , Online information services
- Description: The nature of Internet and Intranet Web applications has changed from a static content-distribution medium into an interactive, dynamic medium, often used to serve multimedia from back-end object-relational databases to Web-enabled clients. Consequently, developers need to make an informed technological choice for developing software that supports a Web-based application for distributing multimedia over networks. This decision is based on several factors. Among the factors are ease of programming, richness of features, scalability, and performance. The research focuses on these key factors when distributing images from an Oracle9i database using Java Servlets, JSP, ASP, and ASP.NET as the server-side development technologies. Prototype applications are developed and tested within each technology: one for single image serving and the other for multiple image serving. A matrix of recommendations is provided to distinguish which technology, or combination of technologies, provides the best performance and development platform for image serving within the studied envirorunent. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2004
A contribution to cabbage pest management by subsistence and small-scale farmers in the Eastern Cape, South Africa
- Authors: Mkize, Nolwazi
- Date: 2004
- Subjects: Cabbage -- Diseases and pests , Insect pests -- Integrated control -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5659 , http://hdl.handle.net/10962/d1005342 , Cabbage -- Diseases and pests , Insect pests -- Integrated control -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape
- Description: The interaction between farmers, agricultural scientists and extension workers is sometimes overlooked in agricultural entomology. In an attempt to respond to this reality this study examines some foundation of this interaction in relation to the pest management practices of subsistence and small-scale farmers and also highlights the problems that might arise in the implementation of IPM. Problems involving pests occurrence; language barriers; beliefs, knowledge and perception about insects, and visual literacy are examined. The thesis has a two-fold focus, firstly the study of pests on cabbages of subsistence farmers in Grahamstown and secondly a broader focus on other aspects such as cultural entomology, perception of insects and visual literacy specifically in relation to Xhosa speaking people in the Eastern Cape. The most important crop for emergent farmers in the Eastern Cape are cabbages, which have a variety of pests of which diamondback moths and are the most important. Traditional pest management practices tend to influence the development of IPM programmes adopted by these farmers. Eastern Cape farmers apply periodic cropping systems, which had an effect on the population densities of diamondback moth (DBM), other lepidopteran pests and their parasitoids. Considering the maximum population densities of DBM, which were 0.2 - 2.9 larvae/plant, there were no major pest problems. The availability of parasitoids, even in highly disturbed and patchy environments, showed good potential for biological control. Since some extension officers cannot speak the local farmers’ language, a dictionary of insect names was formulated in their language (isiXhosa) to assist communication. Response-frequency distribution analysis showed that the dictionary is essentially complete. The literal translations of some names show that isiXhosa speakers often relate insects to people, or to their habitat or classify them according to their behaviour. Farmers from eight sites in the Eastern Cape were interviewed regarding their knowledge and perception of insect pests and their control thereof. To some extent, farmers still rely on cultural control and have beliefs about insects that reflected both reality and superstition. There is no difference between the Ciskei and Transkei regions regarding insect-related beliefs. Farmers generally lack an understanding of insect ecology. There is a need for farmers to be taught about insects to assist with the implementation of IPM. Leftover pesticides from commercial farms or detergents are sometimes used to manage the pests. When training illiterate or semi-literate farmers, it is important to understand their media literacy so as to design useful graphic and object training media. Generally farmers showed that they either understand graphic or object media depending on the features of the insects being looked at. These findings are discussed with regard to the potential development of IPM training material for subsistence and small-scale farmers in a community.
- Full Text:
- Date Issued: 2004
- Authors: Mkize, Nolwazi
- Date: 2004
- Subjects: Cabbage -- Diseases and pests , Insect pests -- Integrated control -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5659 , http://hdl.handle.net/10962/d1005342 , Cabbage -- Diseases and pests , Insect pests -- Integrated control -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape
- Description: The interaction between farmers, agricultural scientists and extension workers is sometimes overlooked in agricultural entomology. In an attempt to respond to this reality this study examines some foundation of this interaction in relation to the pest management practices of subsistence and small-scale farmers and also highlights the problems that might arise in the implementation of IPM. Problems involving pests occurrence; language barriers; beliefs, knowledge and perception about insects, and visual literacy are examined. The thesis has a two-fold focus, firstly the study of pests on cabbages of subsistence farmers in Grahamstown and secondly a broader focus on other aspects such as cultural entomology, perception of insects and visual literacy specifically in relation to Xhosa speaking people in the Eastern Cape. The most important crop for emergent farmers in the Eastern Cape are cabbages, which have a variety of pests of which diamondback moths and are the most important. Traditional pest management practices tend to influence the development of IPM programmes adopted by these farmers. Eastern Cape farmers apply periodic cropping systems, which had an effect on the population densities of diamondback moth (DBM), other lepidopteran pests and their parasitoids. Considering the maximum population densities of DBM, which were 0.2 - 2.9 larvae/plant, there were no major pest problems. The availability of parasitoids, even in highly disturbed and patchy environments, showed good potential for biological control. Since some extension officers cannot speak the local farmers’ language, a dictionary of insect names was formulated in their language (isiXhosa) to assist communication. Response-frequency distribution analysis showed that the dictionary is essentially complete. The literal translations of some names show that isiXhosa speakers often relate insects to people, or to their habitat or classify them according to their behaviour. Farmers from eight sites in the Eastern Cape were interviewed regarding their knowledge and perception of insect pests and their control thereof. To some extent, farmers still rely on cultural control and have beliefs about insects that reflected both reality and superstition. There is no difference between the Ciskei and Transkei regions regarding insect-related beliefs. Farmers generally lack an understanding of insect ecology. There is a need for farmers to be taught about insects to assist with the implementation of IPM. Leftover pesticides from commercial farms or detergents are sometimes used to manage the pests. When training illiterate or semi-literate farmers, it is important to understand their media literacy so as to design useful graphic and object training media. Generally farmers showed that they either understand graphic or object media depending on the features of the insects being looked at. These findings are discussed with regard to the potential development of IPM training material for subsistence and small-scale farmers in a community.
- Full Text:
- Date Issued: 2004
A critical analysis of the influence of the "Prevention of the illegal eviction from and unlawful occupation of Land Act 19 of 1998" on investment in residential income-producing property
- Authors: Botha, Brink
- Date: 2004
- Subjects: Rental housing -- Law and legislation -- South Africa , Eviction -- Law and legislation -- South Africa , Occupancy (Law) -- South Africa , Real estate investment -- Law and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11005 , http://hdl.handle.net/10948/321 , Rental housing -- Law and legislation -- South Africa , Eviction -- Law and legislation -- South Africa , Occupancy (Law) -- South Africa , Real estate investment -- Law and legislation -- South Africa
- Description: This research will focus on the influence of legislation (as indicated in this research) on the investment decision in residential income-producing property. Assumptions, as recorded in the hypothesis of this study, indicate that the legislation had a changing influence on the investment decision in residential income-producing property in comparison to the time period prior to the promulgation of the legislation. The research methodology will be based on a comparative analysis of the current legislation and the proposed Draft Amendment Bill. This analysis will be tested by means of a case study analysis incorporating a phenomenological study based on written data. The problems, sub-problems and hypothesis will be addressed and tested in this research in conjunction with the prescribed research methodology. This research is concluded by means of a synopsis and recommendations.
- Full Text:
- Date Issued: 2004
- Authors: Botha, Brink
- Date: 2004
- Subjects: Rental housing -- Law and legislation -- South Africa , Eviction -- Law and legislation -- South Africa , Occupancy (Law) -- South Africa , Real estate investment -- Law and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11005 , http://hdl.handle.net/10948/321 , Rental housing -- Law and legislation -- South Africa , Eviction -- Law and legislation -- South Africa , Occupancy (Law) -- South Africa , Real estate investment -- Law and legislation -- South Africa
- Description: This research will focus on the influence of legislation (as indicated in this research) on the investment decision in residential income-producing property. Assumptions, as recorded in the hypothesis of this study, indicate that the legislation had a changing influence on the investment decision in residential income-producing property in comparison to the time period prior to the promulgation of the legislation. The research methodology will be based on a comparative analysis of the current legislation and the proposed Draft Amendment Bill. This analysis will be tested by means of a case study analysis incorporating a phenomenological study based on written data. The problems, sub-problems and hypothesis will be addressed and tested in this research in conjunction with the prescribed research methodology. This research is concluded by means of a synopsis and recommendations.
- Full Text:
- Date Issued: 2004
A geological evaluation of marine diamond placer deposits on the central Namibian inner shelf : a case study of the Hottentot Bay area
- Authors: Rau, Grant
- Date: 2004 , 2013-06-03
- Subjects: Diamond mines and mining -- Namibia , Ocean mining -- Namibia , Marine mineral resources
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5038 , http://hdl.handle.net/10962/d1007554 , Diamond mines and mining -- Namibia , Ocean mining -- Namibia , Marine mineral resources
- Description: This study focusses on the marine diamond placers within Exclusive Prospecting Licence 1950 and Mining Licence 103a, located northwest of the north-facing Hottentot Bay which is 60 km north of Lüderitz, along the central Namibian coastline. The thesis follows the natural geological evolution of the marine placer deposit from primary source, through alluvial and/or glacial transportation, concentration along the coastline by wave, aeolian and alluvial/sheet-wash processes and finally marine diamond placer preservation. All of these processes are reviewed as they are important in understanding of the evolution marine placer deposits. The poly-cyclic role of coastal aeolian, alluvial, and marine processes, in marine placer enrichment is shown to be particularly important in considered target identification and prioritisation. A detailed bathymetric, sonographic and seismic interpretation, is an integral part of diamond placer exploration, and was used to examine and describe surficial and sub-bottom characteristics within the study area. Marine placers are formed along palaeo-strandlines during periods of marine transgression and regression and are therefore fundamental in marine placer exploration. A detailed bathymetry map, compiled for this study, of the area between Lüderitz Bay and Clara Hill, provides the foundation for a detailed terrace level investigation. Regionally, twelve well-developed stillstand levels are identified, nine of which fall into the study area. These interpretations are compared with global eustatic as well as terrace and resource/reserve levels in the Lüderitz area and are found to correlate well. Sediment dynamic studies involve the use of accredited application software for wave refraction modelling, to determine the wave angle and orbital wave velocity at the seabed. Bedload velocities, required to move diamonds of specific sizes, can be empirically determined and therefore areas of diamond entrainment and deposition can be modelled and target features delineated and prioritised. These detailed interpretations provide a sound platform for evaluating diamond placer process models in the study area. By integrating both previously published and newly formulated ideas, a revised, holistic model for the formation of marine diamond placer deposits in central Namibian is postulated. The proposed model is tested by comparing it to the lateral distribution of presently defined resource/reserve areas in the Lüderitz area and shows a close correlation with most of these enriched deposits. Based on this model, a matrix for the delineation and prioritisation of marine placer deposits is developed and the best target features within the study area are identified. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2004
- Authors: Rau, Grant
- Date: 2004 , 2013-06-03
- Subjects: Diamond mines and mining -- Namibia , Ocean mining -- Namibia , Marine mineral resources
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5038 , http://hdl.handle.net/10962/d1007554 , Diamond mines and mining -- Namibia , Ocean mining -- Namibia , Marine mineral resources
- Description: This study focusses on the marine diamond placers within Exclusive Prospecting Licence 1950 and Mining Licence 103a, located northwest of the north-facing Hottentot Bay which is 60 km north of Lüderitz, along the central Namibian coastline. The thesis follows the natural geological evolution of the marine placer deposit from primary source, through alluvial and/or glacial transportation, concentration along the coastline by wave, aeolian and alluvial/sheet-wash processes and finally marine diamond placer preservation. All of these processes are reviewed as they are important in understanding of the evolution marine placer deposits. The poly-cyclic role of coastal aeolian, alluvial, and marine processes, in marine placer enrichment is shown to be particularly important in considered target identification and prioritisation. A detailed bathymetric, sonographic and seismic interpretation, is an integral part of diamond placer exploration, and was used to examine and describe surficial and sub-bottom characteristics within the study area. Marine placers are formed along palaeo-strandlines during periods of marine transgression and regression and are therefore fundamental in marine placer exploration. A detailed bathymetry map, compiled for this study, of the area between Lüderitz Bay and Clara Hill, provides the foundation for a detailed terrace level investigation. Regionally, twelve well-developed stillstand levels are identified, nine of which fall into the study area. These interpretations are compared with global eustatic as well as terrace and resource/reserve levels in the Lüderitz area and are found to correlate well. Sediment dynamic studies involve the use of accredited application software for wave refraction modelling, to determine the wave angle and orbital wave velocity at the seabed. Bedload velocities, required to move diamonds of specific sizes, can be empirically determined and therefore areas of diamond entrainment and deposition can be modelled and target features delineated and prioritised. These detailed interpretations provide a sound platform for evaluating diamond placer process models in the study area. By integrating both previously published and newly formulated ideas, a revised, holistic model for the formation of marine diamond placer deposits in central Namibian is postulated. The proposed model is tested by comparing it to the lateral distribution of presently defined resource/reserve areas in the Lüderitz area and shows a close correlation with most of these enriched deposits. Based on this model, a matrix for the delineation and prioritisation of marine placer deposits is developed and the best target features within the study area are identified. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2004
A statistical evaluation and analysis of mosquito repellent combination
- Authors: Asquith, Ilse Bernadette
- Date: 2004
- Subjects: Mosquitoes -- Control , Insect baits and repellents
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10422 , http://hdl.handle.net/10948/d1015732
- Description: The present product development project was aimed at studying the synergism and/or antagonism amongst various known insect repellent actives with the view to formulating a multi-active repellent product with improved properties when compared to current single-active commercial products. Advanced statistical methods were used to identify synergism between individual active substances and to define a formulation as close as possible to the “ideal” formulation. Several mosquito repellent samples were prepared and sent to the South African Bureau of Standards (SABS) in Pretoria to test for their efficiency in repelling mosquitoes. From the results of the repellency tests of the various active combinations, three actives were identified that showed promising signs of synergism. These actives were then studied in further detail to determine their optimum combination. In addition, it was shown that when using a natural flavourant as promoter and incorporating a slow-release agent into formulations for aerosols and lotions, a product is obtained that gives comparable levels of efficiency to current commercial products, but at much reduced levels of active loading. Accelerated stability tests performed on the final combination of the three actives used in the final formulation showed no adverse reactions over a three-week study. These tests shall be repeated once the final application form (lotion, aerosol, etc) and product packing have been decided.
- Full Text:
- Date Issued: 2004
- Authors: Asquith, Ilse Bernadette
- Date: 2004
- Subjects: Mosquitoes -- Control , Insect baits and repellents
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10422 , http://hdl.handle.net/10948/d1015732
- Description: The present product development project was aimed at studying the synergism and/or antagonism amongst various known insect repellent actives with the view to formulating a multi-active repellent product with improved properties when compared to current single-active commercial products. Advanced statistical methods were used to identify synergism between individual active substances and to define a formulation as close as possible to the “ideal” formulation. Several mosquito repellent samples were prepared and sent to the South African Bureau of Standards (SABS) in Pretoria to test for their efficiency in repelling mosquitoes. From the results of the repellency tests of the various active combinations, three actives were identified that showed promising signs of synergism. These actives were then studied in further detail to determine their optimum combination. In addition, it was shown that when using a natural flavourant as promoter and incorporating a slow-release agent into formulations for aerosols and lotions, a product is obtained that gives comparable levels of efficiency to current commercial products, but at much reduced levels of active loading. Accelerated stability tests performed on the final combination of the three actives used in the final formulation showed no adverse reactions over a three-week study. These tests shall be repeated once the final application form (lotion, aerosol, etc) and product packing have been decided.
- Full Text:
- Date Issued: 2004
Age, growth and yield-per-recruit analysis of ndunduma Diplotaxodon limnothrissa (Teleostei: Cichlidae), in the southeastern arm of Lake Malawi
- Authors: Kanyerere, Geoffrey Zantute
- Date: 2004
- Subjects: Fishes -- Nyasa, Lake , Cichlids -- Nyasa, Lake , Fishes -- Growth -- Nyasa, Lake , Fishes -- Age -- Nyasa, Lake , Fishery resources -- Malawi , Fishery management -- Malawi
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5214 , http://hdl.handle.net/10962/d1005057 , Fishes -- Nyasa, Lake , Cichlids -- Nyasa, Lake , Fishes -- Growth -- Nyasa, Lake , Fishes -- Age -- Nyasa, Lake , Fishery resources -- Malawi , Fishery management -- Malawi
- Description: Diplotaxodon limnothrissa Turner (1995) is a widely distributed species occurring throughout Lake Malawi, extending from the surface to a depth of at least 220m. It is probably the most abundant cichlid in the lake with biomass estimates of around 87 000 tonnes in the pelagic zone alone. The species is exploited commercially in the southern part of the lake but since its inception the fishery has never been assessed. As such this study investigates some aspects of age and growth of the species besides applying a yield-per-recruit analysis to assess the status of the fishery. Analysis of sectioned sagittal otoliths revealed that D. limnothrissa is fast growing and relatively long-lived species, attaining ages in excess of 10 years. Growth in length was rapid in immature fish, with fish attaining almost half of their maximum size within their first year. Le ngth-at-age was described by the von Bertalanffy growth model with combined-sex growth described as Lt = 211.21(1-exp(- 0.24(t+1.36))) mm TL. Total, natural and fishing mortalities were estimated at 0.76 yr⁻¹, 0.31 yr⁻¹ and 0.45 yr⁻¹ respectively. Per-recruit analysis indicated that the D. limnothrissa stock in the southeast arm of the lake is fully exploited as indicated by the current spawner biomass-per-recruit ratios of 31-55% (SB/R)F=0. Modelling indicated that the current age-at-capture (2.67 years) is lower than the age at which yield is optimised (> 5 years) based on the F₀·₁ harvesting strategy. It is, therefore, recommended that the age-at-capture should be increased from 2.67 to 5 years to optimise yield.
- Full Text:
- Date Issued: 2004
- Authors: Kanyerere, Geoffrey Zantute
- Date: 2004
- Subjects: Fishes -- Nyasa, Lake , Cichlids -- Nyasa, Lake , Fishes -- Growth -- Nyasa, Lake , Fishes -- Age -- Nyasa, Lake , Fishery resources -- Malawi , Fishery management -- Malawi
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5214 , http://hdl.handle.net/10962/d1005057 , Fishes -- Nyasa, Lake , Cichlids -- Nyasa, Lake , Fishes -- Growth -- Nyasa, Lake , Fishes -- Age -- Nyasa, Lake , Fishery resources -- Malawi , Fishery management -- Malawi
- Description: Diplotaxodon limnothrissa Turner (1995) is a widely distributed species occurring throughout Lake Malawi, extending from the surface to a depth of at least 220m. It is probably the most abundant cichlid in the lake with biomass estimates of around 87 000 tonnes in the pelagic zone alone. The species is exploited commercially in the southern part of the lake but since its inception the fishery has never been assessed. As such this study investigates some aspects of age and growth of the species besides applying a yield-per-recruit analysis to assess the status of the fishery. Analysis of sectioned sagittal otoliths revealed that D. limnothrissa is fast growing and relatively long-lived species, attaining ages in excess of 10 years. Growth in length was rapid in immature fish, with fish attaining almost half of their maximum size within their first year. Le ngth-at-age was described by the von Bertalanffy growth model with combined-sex growth described as Lt = 211.21(1-exp(- 0.24(t+1.36))) mm TL. Total, natural and fishing mortalities were estimated at 0.76 yr⁻¹, 0.31 yr⁻¹ and 0.45 yr⁻¹ respectively. Per-recruit analysis indicated that the D. limnothrissa stock in the southeast arm of the lake is fully exploited as indicated by the current spawner biomass-per-recruit ratios of 31-55% (SB/R)F=0. Modelling indicated that the current age-at-capture (2.67 years) is lower than the age at which yield is optimised (> 5 years) based on the F₀·₁ harvesting strategy. It is, therefore, recommended that the age-at-capture should be increased from 2.67 to 5 years to optimise yield.
- Full Text:
- Date Issued: 2004
Algorithms for the solution of the quadratic programming problem
- Authors: Vankova, Martina
- Date: 2004
- Subjects: Quadratic programming , Nonlinear programming , Algorithms
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11086 , http://hdl.handle.net/10948/348 , Quadratic programming , Nonlinear programming , Algorithms
- Description: The purpose of this dissertation was to provide a review of the theory of Optimization, in particular quadratic programming, and the algorithms suitable for solving both convex and non-convex quadratic programming problems. Optimization problems arise in a wide variety of fields and many can be effectively modeled with linear equations. However, there are problems for which linear models are not sufficient thus creating a need for non-linear systems. This dissertation includes a literature study of the formal theory necessary for understanding optimization and an investigation of the algorithms available for solving a special class of the non-linear programming problem, namely the quadratic programming problem. It was not the intention of this dissertation to discuss all possible algorithms for solving the quadratic programming problem, therefore certain algorithms for convex and non-convex quadratic programming problems were selected for a detailed discussion in the dissertation. Some of the algorithms were selected arbitrarily, because limited information was available comparing the efficiency of the various algorithms. Algorithms available for solving general non-linear programming problems were also included and briefly discussed as they can be used to solve quadratic programming problems. A number of algorithms were then selected for evaluation, depending on the frequency of use in practice and the availability of software implementing these algorithms. The evaluation included a theoretical and quantitative comparison of the algorithms. The quantitative results were analyzed and discussed and it was shown that the results supported the theoretical comparison. It was also shown that it is difficult to conclude that one algorithm is better than another as the efficiency of an algorithm greatly depends on the size of the problem, the complexity of an algorithm and many other implementation issues. Optimization problems arise continuously in a wide range of fields and thus create the need for effective methods of solving them. This dissertation provides the fundamental theory necessary for the understanding of optimization problems, with particular reference to quadratic programming problems and the algorithms that solve such problems. Keywords: Quadratic Programming, Quadratic Programming Algorithms, Optimization, Non-linear Programming, Convex, Non-convex.
- Full Text:
- Date Issued: 2004
- Authors: Vankova, Martina
- Date: 2004
- Subjects: Quadratic programming , Nonlinear programming , Algorithms
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11086 , http://hdl.handle.net/10948/348 , Quadratic programming , Nonlinear programming , Algorithms
- Description: The purpose of this dissertation was to provide a review of the theory of Optimization, in particular quadratic programming, and the algorithms suitable for solving both convex and non-convex quadratic programming problems. Optimization problems arise in a wide variety of fields and many can be effectively modeled with linear equations. However, there are problems for which linear models are not sufficient thus creating a need for non-linear systems. This dissertation includes a literature study of the formal theory necessary for understanding optimization and an investigation of the algorithms available for solving a special class of the non-linear programming problem, namely the quadratic programming problem. It was not the intention of this dissertation to discuss all possible algorithms for solving the quadratic programming problem, therefore certain algorithms for convex and non-convex quadratic programming problems were selected for a detailed discussion in the dissertation. Some of the algorithms were selected arbitrarily, because limited information was available comparing the efficiency of the various algorithms. Algorithms available for solving general non-linear programming problems were also included and briefly discussed as they can be used to solve quadratic programming problems. A number of algorithms were then selected for evaluation, depending on the frequency of use in practice and the availability of software implementing these algorithms. The evaluation included a theoretical and quantitative comparison of the algorithms. The quantitative results were analyzed and discussed and it was shown that the results supported the theoretical comparison. It was also shown that it is difficult to conclude that one algorithm is better than another as the efficiency of an algorithm greatly depends on the size of the problem, the complexity of an algorithm and many other implementation issues. Optimization problems arise continuously in a wide range of fields and thus create the need for effective methods of solving them. This dissertation provides the fundamental theory necessary for the understanding of optimization problems, with particular reference to quadratic programming problems and the algorithms that solve such problems. Keywords: Quadratic Programming, Quadratic Programming Algorithms, Optimization, Non-linear Programming, Convex, Non-convex.
- Full Text:
- Date Issued: 2004
An assessment of density estimation methods for forest ungulates
- Authors: Ellis, Amanda Morgan
- Date: 2004
- Subjects: Wildlife management -- South Africa -- Eastern Cape Animal populations -- Estimates Wildlife conservation -- South Africa -- Eastern Cape Game farms -- South Africa -- Eastern Cape Ungulates -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5829 , http://hdl.handle.net/10962/d1007830
- Description: The development of conservation and management programs for an animal population relies on a knowledge of the number of individuals in an area. In order to achieve reliable estimates, precise and accurate techniques for estimating population densities are needed. This study compared the use of direct and indirect methods of estimating kudu (Trage/aphus strepsiceras), bush buck (Trage/aphus scriptus), common duiker (Sy/vicapra grimmia), and blue duiker (Philantamba manticala) densities on Shamwari Game Reserve in the Eastern Cape Province, South Africa. These species prefer habitats of dense forest and bush for concealment and are therefore not easily counted in open areas. Herein direct observation counts were compared to indirect sampling via pellet group counts (clearance plots, line transects, variable-width transects, and strip transects). Clearance plots were examined every 2 weeks, while all other methods were conducted seasonally, from August 2002 until August 2003. The strip transect method provided the lowest density estimates (animals per hal ranging from 0.001 for bushbuck to 0.025 for common duiker, while direct observations yielded the highest estimates, ranging from 0.804 for bush buck to 4.692 for kudu. Also, a validation of methods was performed against a known population of kudu during which the DISTANCE method yielded the most accurate results, with an estimated density of 0.261 that was within the actual density of 0.246 to 0.282. In addition, the DISTANCE method was compared to helicopter counts ofkudu and its estimates were found to be approximately 2.6 times greater than the helicopter count results. When the assessment of the methods was made, the cost, manpower and effort requirements, coefficient of variation, and performance against a known population for each method were taken into consideration. Overall, the DISTANCE method performed the best with low cost, minimal manpower and effort requirements, and low coefficient of variation. On Shamwari Game Reserve, the DISTANCE method estimated 0.300 kudu, 0.108 bushbuck, 0.387 common duiker, and 0.028 blue duiker per ha, which, when extrapolated to the total number of animals present within subtropical thicket habitat, estimated 1973 kudu, 710 bush buck, 2545 common duiker, and 184 blue duiker.
- Full Text:
- Date Issued: 2004
- Authors: Ellis, Amanda Morgan
- Date: 2004
- Subjects: Wildlife management -- South Africa -- Eastern Cape Animal populations -- Estimates Wildlife conservation -- South Africa -- Eastern Cape Game farms -- South Africa -- Eastern Cape Ungulates -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5829 , http://hdl.handle.net/10962/d1007830
- Description: The development of conservation and management programs for an animal population relies on a knowledge of the number of individuals in an area. In order to achieve reliable estimates, precise and accurate techniques for estimating population densities are needed. This study compared the use of direct and indirect methods of estimating kudu (Trage/aphus strepsiceras), bush buck (Trage/aphus scriptus), common duiker (Sy/vicapra grimmia), and blue duiker (Philantamba manticala) densities on Shamwari Game Reserve in the Eastern Cape Province, South Africa. These species prefer habitats of dense forest and bush for concealment and are therefore not easily counted in open areas. Herein direct observation counts were compared to indirect sampling via pellet group counts (clearance plots, line transects, variable-width transects, and strip transects). Clearance plots were examined every 2 weeks, while all other methods were conducted seasonally, from August 2002 until August 2003. The strip transect method provided the lowest density estimates (animals per hal ranging from 0.001 for bushbuck to 0.025 for common duiker, while direct observations yielded the highest estimates, ranging from 0.804 for bush buck to 4.692 for kudu. Also, a validation of methods was performed against a known population of kudu during which the DISTANCE method yielded the most accurate results, with an estimated density of 0.261 that was within the actual density of 0.246 to 0.282. In addition, the DISTANCE method was compared to helicopter counts ofkudu and its estimates were found to be approximately 2.6 times greater than the helicopter count results. When the assessment of the methods was made, the cost, manpower and effort requirements, coefficient of variation, and performance against a known population for each method were taken into consideration. Overall, the DISTANCE method performed the best with low cost, minimal manpower and effort requirements, and low coefficient of variation. On Shamwari Game Reserve, the DISTANCE method estimated 0.300 kudu, 0.108 bushbuck, 0.387 common duiker, and 0.028 blue duiker per ha, which, when extrapolated to the total number of animals present within subtropical thicket habitat, estimated 1973 kudu, 710 bush buck, 2545 common duiker, and 184 blue duiker.
- Full Text:
- Date Issued: 2004
An evaluation of paired comparison models
- Venter, Daniel Jacobus Lodewyk
- Authors: Venter, Daniel Jacobus Lodewyk
- Date: 2004
- Subjects: Paired comparisons (Statistics) , Mathematical statistics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11087 , http://hdl.handle.net/10948/364 , Paired comparisons (Statistics) , Mathematical statistics
- Description: Introduction: A typical task in quantitative data analysis is to derive estimates of population parameters based on sample statistics. For manifest variables this is usually a straightforward process utilising suitable measurement instruments and standard statistics such the mean, median and standard deviation. Latent variables on the other hand are typically more elusive, making it difficult to obtain valid and reliable measurements. One of the most widely used methods of estimating the parameter value of a latent variable is to use a summated score derived from a set of individual scores for each of the various attributes of the latent variable. A serious limitation of this method and other similar methods is that the validity and reliability of measurements depend on whether the statements included in the questionnaire cover all characteristics of the variable being measured and also on respondents’ ability to correctly indicate their perceived assessment of the characteristics on the scale provided. Methods without this limitation and that are especially useful where a set of objects/entities must be ranked based on the parameter values of one or more latent variables, are methods of paired comparisons. Although the underlying assumptions and algorithms of these methods often differ dramatically, they all rely on data derived from a series of comparisons, each consisting of a pair of specimens selected from the set of objects/entities being investigated. Typical examples of the comparison process are: subjects (judges) who have to indicate for each pair of objects which of the two they prefer; sport teams that compete against each other in matches that involve two teams at a time. The resultant data of each comparison range from a simple dichotomy to indicate which of the two objects are preferred/better, to an interval or ratio scale score for e d Bradley-Terry models, and were based on statistical theory assuming that the variable(s) being measured is either normally (Thurstone-Mosteller) or exponentially (Bradley-Terry) distributed. For many years researchers had to rely on these PCM’s when analysing paired comparison data without any idea about the implications if the distribution of the data from which their sample were obtained differed from the assumed distribution for the applicable PCM being utilised. To address this problem, PCM’s were subsequently developed to cater for discrete variables and variables with distributions that are neither normal or exponential. A question that remained unanswered is how the performance, as measured by the accuracy of parameter estimates, of PCM's are affected if they are applied to data from a range of discrete and continuous distribution that violates the assumptions on which the applicable paired comparison algorithm is based. This study is an attempt to answer this question by applying the most popular PCM's to a range of randomly derived data sets that spans typical continuous and discrete data distributions. It is hoped that the results of this study will assist researchers when selecting the most appropriate PCM to obtain accurate estimates of the parameters of the variables in their data sets.
- Full Text:
- Date Issued: 2004
- Authors: Venter, Daniel Jacobus Lodewyk
- Date: 2004
- Subjects: Paired comparisons (Statistics) , Mathematical statistics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11087 , http://hdl.handle.net/10948/364 , Paired comparisons (Statistics) , Mathematical statistics
- Description: Introduction: A typical task in quantitative data analysis is to derive estimates of population parameters based on sample statistics. For manifest variables this is usually a straightforward process utilising suitable measurement instruments and standard statistics such the mean, median and standard deviation. Latent variables on the other hand are typically more elusive, making it difficult to obtain valid and reliable measurements. One of the most widely used methods of estimating the parameter value of a latent variable is to use a summated score derived from a set of individual scores for each of the various attributes of the latent variable. A serious limitation of this method and other similar methods is that the validity and reliability of measurements depend on whether the statements included in the questionnaire cover all characteristics of the variable being measured and also on respondents’ ability to correctly indicate their perceived assessment of the characteristics on the scale provided. Methods without this limitation and that are especially useful where a set of objects/entities must be ranked based on the parameter values of one or more latent variables, are methods of paired comparisons. Although the underlying assumptions and algorithms of these methods often differ dramatically, they all rely on data derived from a series of comparisons, each consisting of a pair of specimens selected from the set of objects/entities being investigated. Typical examples of the comparison process are: subjects (judges) who have to indicate for each pair of objects which of the two they prefer; sport teams that compete against each other in matches that involve two teams at a time. The resultant data of each comparison range from a simple dichotomy to indicate which of the two objects are preferred/better, to an interval or ratio scale score for e d Bradley-Terry models, and were based on statistical theory assuming that the variable(s) being measured is either normally (Thurstone-Mosteller) or exponentially (Bradley-Terry) distributed. For many years researchers had to rely on these PCM’s when analysing paired comparison data without any idea about the implications if the distribution of the data from which their sample were obtained differed from the assumed distribution for the applicable PCM being utilised. To address this problem, PCM’s were subsequently developed to cater for discrete variables and variables with distributions that are neither normal or exponential. A question that remained unanswered is how the performance, as measured by the accuracy of parameter estimates, of PCM's are affected if they are applied to data from a range of discrete and continuous distribution that violates the assumptions on which the applicable paired comparison algorithm is based. This study is an attempt to answer this question by applying the most popular PCM's to a range of randomly derived data sets that spans typical continuous and discrete data distributions. It is hoped that the results of this study will assist researchers when selecting the most appropriate PCM to obtain accurate estimates of the parameters of the variables in their data sets.
- Full Text:
- Date Issued: 2004
An investigation into the captive spawning, egg characteristics and egg quality of the mud crab (Scylla serrata) in South Africa
- Authors: Churchill, Giles John
- Date: 2004
- Subjects: Scylla serrata , Crabs -- South Africa , Scylla serrata -- South Africa , Crabs -- Eggs -- Incubation , Crabs -- Spawning
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5376 , http://hdl.handle.net/10962/d1015963
- Description: The source of a reliable supply of seed is one of the most important factors determining the success of aquaculture. At present mud crab culture is reliant on wild caught juveniles, which limits the expansion of mud crab farming globally. This, coupled with the paucity of knowledge of the spawning and egg characteristics of mud crabs, prompted the investigation into the captive spawning, incubation and egg quality of Scylla serrata in South Africa. A total of 112 mature females were caught from three estuaries in northern KwaZulu Natal and acclimated to captive conditions. Mature females were present in the estuaries throughout the year and ranged from 90 to 200 mm carapace width. Except for 40 crabs all the females spawned in captivity. On average spawning took place 38 ± 23 days after capture but was not dependent on crab size. Fecundity was significantly correlated to crab size (p = 0.026), with larger females producing more eggs. The average fecundity per batch was 5.79 ± 2.07 million eggs. Hatch success rate for all egg batches was high averaging 84 ± 6 %. Egg dropping occurred in all egg batches during incubation. On average 6.5 % of the egg mass was dropped during incubation. At the average temperature of 27⁰C eggs in the incubation system hatched within 292 ± 12 hours after extrusion. In-vitro incubation of eggs proved to be an ineffective means of incubating Scylla serrata eggs with a hatch success rate of only 25 ± 5 %. Egg quality was assessed both directly, using variables such as hatch success rate and hatch synchronicity and retrospectively by using stress tests and comparing survival of larvae from different coloured egg batches. Lipid class composition and fatty acid methyl ester content for 28 batches of eggs and 16 batches of larvae were analyzed. The biochemical composition of the egg was correlated to survival of larvae in the stress tests to ascertain if the lipid content of the eggs determines their quality. Stress tests were used to retrospectively assess egg quality. Four stressors were administered to newly hatched larvae and the LD50 values recorded. An average LD50 of 64 hours was recorded for the starvation tests, 40% for the salinity tests, 37 ppm for the formalin tests and 39.7 mg/l NH3 for the ammonia stress tests was recorded. Egg colour ranged from pale yellow to orange-red. Egg colour was not influenced by female size or the time the females spent in captivity prior to spawning. Egg colour can therefore not be used as an indicator of quality. The lipid profiles of newly extruded eggs had no effect on hatch synchronicity or hatch success rate. However, the stress tests identified female size (p ≤ 0.02), DHA content of the egg (p ≤ 0.02), Σ omega-3 fatty acids of eggs (p ≤ 0.02) and EPA content of eggs (p ≤ 0.007) as possible determinants of egg quality. Larger crabs tended to produce poorer quality eggs. Egg quality also decreased as the amount of DHA, EPA and the Σ omega-3 fatty acids decreased in the eggs. To summarize, the results of this study indicated that the following parameters affect egg quality - crab size, DHA, EPA and Σ omega-3 fatty acid content of eggs.
- Full Text:
- Date Issued: 2004
- Authors: Churchill, Giles John
- Date: 2004
- Subjects: Scylla serrata , Crabs -- South Africa , Scylla serrata -- South Africa , Crabs -- Eggs -- Incubation , Crabs -- Spawning
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5376 , http://hdl.handle.net/10962/d1015963
- Description: The source of a reliable supply of seed is one of the most important factors determining the success of aquaculture. At present mud crab culture is reliant on wild caught juveniles, which limits the expansion of mud crab farming globally. This, coupled with the paucity of knowledge of the spawning and egg characteristics of mud crabs, prompted the investigation into the captive spawning, incubation and egg quality of Scylla serrata in South Africa. A total of 112 mature females were caught from three estuaries in northern KwaZulu Natal and acclimated to captive conditions. Mature females were present in the estuaries throughout the year and ranged from 90 to 200 mm carapace width. Except for 40 crabs all the females spawned in captivity. On average spawning took place 38 ± 23 days after capture but was not dependent on crab size. Fecundity was significantly correlated to crab size (p = 0.026), with larger females producing more eggs. The average fecundity per batch was 5.79 ± 2.07 million eggs. Hatch success rate for all egg batches was high averaging 84 ± 6 %. Egg dropping occurred in all egg batches during incubation. On average 6.5 % of the egg mass was dropped during incubation. At the average temperature of 27⁰C eggs in the incubation system hatched within 292 ± 12 hours after extrusion. In-vitro incubation of eggs proved to be an ineffective means of incubating Scylla serrata eggs with a hatch success rate of only 25 ± 5 %. Egg quality was assessed both directly, using variables such as hatch success rate and hatch synchronicity and retrospectively by using stress tests and comparing survival of larvae from different coloured egg batches. Lipid class composition and fatty acid methyl ester content for 28 batches of eggs and 16 batches of larvae were analyzed. The biochemical composition of the egg was correlated to survival of larvae in the stress tests to ascertain if the lipid content of the eggs determines their quality. Stress tests were used to retrospectively assess egg quality. Four stressors were administered to newly hatched larvae and the LD50 values recorded. An average LD50 of 64 hours was recorded for the starvation tests, 40% for the salinity tests, 37 ppm for the formalin tests and 39.7 mg/l NH3 for the ammonia stress tests was recorded. Egg colour ranged from pale yellow to orange-red. Egg colour was not influenced by female size or the time the females spent in captivity prior to spawning. Egg colour can therefore not be used as an indicator of quality. The lipid profiles of newly extruded eggs had no effect on hatch synchronicity or hatch success rate. However, the stress tests identified female size (p ≤ 0.02), DHA content of the egg (p ≤ 0.02), Σ omega-3 fatty acids of eggs (p ≤ 0.02) and EPA content of eggs (p ≤ 0.007) as possible determinants of egg quality. Larger crabs tended to produce poorer quality eggs. Egg quality also decreased as the amount of DHA, EPA and the Σ omega-3 fatty acids decreased in the eggs. To summarize, the results of this study indicated that the following parameters affect egg quality - crab size, DHA, EPA and Σ omega-3 fatty acid content of eggs.
- Full Text:
- Date Issued: 2004
An investigation into the neuroprotective effects of melatonin in a model of rotenone-induced neurodegeneration
- Authors: Kadanthode, Rubina John
- Date: 2004
- Subjects: Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3763 , http://hdl.handle.net/10962/d1003241 , Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Description: Parkinson’s disease, one of the most common neurodegenerative disorders associated with ageing, is characterised by abnormal and profound loss of nigrostriatal dopaminergic neurons. The cause of Parkinson’s disease is unknown, but epidemiological studies suggest an association with pesticides and other environmental toxins, and biochemical studies implicate oxidative damage and mitochondrial impairment, particularly at the level of complex I enzyme. Recently, rotenone, a commonly used organic pesticide and a classical inhibitor of mitochondrial complex I has been reported to reproduce the specific features of Parkinson’s disease in rodents. The mitochondrial respiratory chain is one of the most important sites of reactive oxygen species production under physiological conditions. Toxic free radicals have been implicated in a variety of neurodegenerative diseases as well as ageing itself. Melatonin, a secretory product of the pineal gland is a multifaceted free radical scavenger and natural antioxidant. In the present study, the neuroprotective effects of melatonin against the environmental neurotoxin, rotenone was investigated. Initial studies showed that inhibition of mitochondrial complex I enzyme by rotenone induced superoxide radical generation. Melatonin, administered to the rat in vivo and in vitro was able to offer neuroprotection by curtailing the production of superoxide radicals induced by rotenone. Mitochondria, being the major target of rotenone, the effects of melatonin were investigated at the mitochondrial level. Melatonin was able to increase the electron transport chain activity thus preventing the respiratory inhibition by rotenone. The pineal hormone also counteracted the action of rotenone on complex I enzyme. These results suggest melatonin’s ability to potentially limit the free radical generation and thereby modulate the mitochondrial functions. The detection and measurement of lipid peroxidation is the evidence most frequently cited to support the involvement of free radical reactions in toxicology and in human disease. Melatonin also offered significant protection in vivo and in vitro against rotenone induced lipid peroxidation. Since iron plays a major role in oxidative damage and in the progression of Parkinson’s disease, the effect of melatonin on both rotenone and iron induced lipid peroxidation was investigated, the results of which show that melatonin affords protection and this was suggested to be due to its interaction with the rotenone-iron complex that might have formed. Electrochemical studies were further used to characterise the interactions between melatonin, rotenone and iron (III). Melatonin was shown to bind with iron and thus reducing their toxicity. Histological studies were undertaken to assess the effects of melatonin on rotenone induced toxicity on the dopaminergic neurons in the rat brain. Rotenone treated brains showed extensive neuronal damage whereas with melatonin less damage was observed. Rotenone induces apoptosis via reactive oxygen species production and apoptotic cell death has been identified in PD brains. Furthermore, the apoptotic cell death was detected and quantified by the TUNEL staining. Rotenone treated sections showed signs of apoptosis whereas with melatonin, less apoptotic damage was observed. The findings of this study indicate that the neurohormone, melatonin may protect against rotenone-induced neurodegeneration. Since melatonin production falls substantially during ageing, the loss of this antioxidant is theorized to be instrumental in the degenerative processes associated with advanced age. Considering how devastating diseases such as Parkinson’s disease, are to a patient and the patient’s families, the discovery of protective agents are a matter of urgency. Further investigations using the pesticide model will help to determine the involvement of environmental exposure in the pathogenesis of human diseases as well as to test therapeutic strategies for the treatment of such diseases.
- Full Text:
- Date Issued: 2004
- Authors: Kadanthode, Rubina John
- Date: 2004
- Subjects: Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3763 , http://hdl.handle.net/10962/d1003241 , Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Description: Parkinson’s disease, one of the most common neurodegenerative disorders associated with ageing, is characterised by abnormal and profound loss of nigrostriatal dopaminergic neurons. The cause of Parkinson’s disease is unknown, but epidemiological studies suggest an association with pesticides and other environmental toxins, and biochemical studies implicate oxidative damage and mitochondrial impairment, particularly at the level of complex I enzyme. Recently, rotenone, a commonly used organic pesticide and a classical inhibitor of mitochondrial complex I has been reported to reproduce the specific features of Parkinson’s disease in rodents. The mitochondrial respiratory chain is one of the most important sites of reactive oxygen species production under physiological conditions. Toxic free radicals have been implicated in a variety of neurodegenerative diseases as well as ageing itself. Melatonin, a secretory product of the pineal gland is a multifaceted free radical scavenger and natural antioxidant. In the present study, the neuroprotective effects of melatonin against the environmental neurotoxin, rotenone was investigated. Initial studies showed that inhibition of mitochondrial complex I enzyme by rotenone induced superoxide radical generation. Melatonin, administered to the rat in vivo and in vitro was able to offer neuroprotection by curtailing the production of superoxide radicals induced by rotenone. Mitochondria, being the major target of rotenone, the effects of melatonin were investigated at the mitochondrial level. Melatonin was able to increase the electron transport chain activity thus preventing the respiratory inhibition by rotenone. The pineal hormone also counteracted the action of rotenone on complex I enzyme. These results suggest melatonin’s ability to potentially limit the free radical generation and thereby modulate the mitochondrial functions. The detection and measurement of lipid peroxidation is the evidence most frequently cited to support the involvement of free radical reactions in toxicology and in human disease. Melatonin also offered significant protection in vivo and in vitro against rotenone induced lipid peroxidation. Since iron plays a major role in oxidative damage and in the progression of Parkinson’s disease, the effect of melatonin on both rotenone and iron induced lipid peroxidation was investigated, the results of which show that melatonin affords protection and this was suggested to be due to its interaction with the rotenone-iron complex that might have formed. Electrochemical studies were further used to characterise the interactions between melatonin, rotenone and iron (III). Melatonin was shown to bind with iron and thus reducing their toxicity. Histological studies were undertaken to assess the effects of melatonin on rotenone induced toxicity on the dopaminergic neurons in the rat brain. Rotenone treated brains showed extensive neuronal damage whereas with melatonin less damage was observed. Rotenone induces apoptosis via reactive oxygen species production and apoptotic cell death has been identified in PD brains. Furthermore, the apoptotic cell death was detected and quantified by the TUNEL staining. Rotenone treated sections showed signs of apoptosis whereas with melatonin, less apoptotic damage was observed. The findings of this study indicate that the neurohormone, melatonin may protect against rotenone-induced neurodegeneration. Since melatonin production falls substantially during ageing, the loss of this antioxidant is theorized to be instrumental in the degenerative processes associated with advanced age. Considering how devastating diseases such as Parkinson’s disease, are to a patient and the patient’s families, the discovery of protective agents are a matter of urgency. Further investigations using the pesticide model will help to determine the involvement of environmental exposure in the pathogenesis of human diseases as well as to test therapeutic strategies for the treatment of such diseases.
- Full Text:
- Date Issued: 2004
Biochemical characterization of plasmodium falciparum heat shock protein 70
- Matambo, Tonderayi Sylvester
- Authors: Matambo, Tonderayi Sylvester
- Date: 2004
- Subjects: Plasmodium falciparum , Malaria -- Prevention , Protein folding , Proteins -- Purification , Heat shock proteins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4134 , http://hdl.handle.net/10962/d1015767
- Description: Plamodium falciparum heat shock protein (PfHsp70) is believed to be involved in the cytoprotection of the malaria parasite through its action as a molecular chaperone. Bioinformatic analysis reveal that PfHsp70 consists of the three canonical Hsp70 domains; an ATPase domain of 45 kDa, Substrate binding domain of 15 kDa and a C-terminal domain of 10 kDa. At the C-terminus there is a GGMP repeat motif that is commonly found in Hsp70s of parasitic origins. Plasmodium falciparum genome is 80% A-T rich, making it difficult to recombinantly express its proteins in Escherhia coli (E. coli) as a result of rare codon usage. In this study we carried out experiments to improve expression in E. coli by inserting the PfHsp70 coding region into the pQE30 expression vector. However multiple bands were detected by Western analysis, probably due to the presence of rare codons. The RIG plasmid, which encodes tRNAs for rare codons in particular Arg (AGA/AGG), Ile (AUA) and Gly (GGA) was engineered into the E. coli strain resulting in production of full length PfHsp70. Purification was achieved through Ni²⁺ Chelating sepharose under denaturing conditions. PfHsp70 was found to have a very low basal ATPase activity of 0.262 ± 0.05 nmoles/min/mg of protein. In the presence of reduced and carboxymethylated lactalbumin (RCMLA) a 11-fold increase in ATPase activity was noted whereas in the presence of both RCMLA and Trypanosoma cruzi DnaJ (Tcj2) a 16-fold was achieved. For ATP hydrolysis kcat value of 0.003 min⁻¹ was obtained whereas for ADP release a greater kcat value of 0.8 min⁻¹ was obtained. These results indicated that rate of ATP hydrolysis maybe the rate-determining step in the ATPase cycle of PfHsp70.
- Full Text:
- Date Issued: 2004
- Authors: Matambo, Tonderayi Sylvester
- Date: 2004
- Subjects: Plasmodium falciparum , Malaria -- Prevention , Protein folding , Proteins -- Purification , Heat shock proteins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4134 , http://hdl.handle.net/10962/d1015767
- Description: Plamodium falciparum heat shock protein (PfHsp70) is believed to be involved in the cytoprotection of the malaria parasite through its action as a molecular chaperone. Bioinformatic analysis reveal that PfHsp70 consists of the three canonical Hsp70 domains; an ATPase domain of 45 kDa, Substrate binding domain of 15 kDa and a C-terminal domain of 10 kDa. At the C-terminus there is a GGMP repeat motif that is commonly found in Hsp70s of parasitic origins. Plasmodium falciparum genome is 80% A-T rich, making it difficult to recombinantly express its proteins in Escherhia coli (E. coli) as a result of rare codon usage. In this study we carried out experiments to improve expression in E. coli by inserting the PfHsp70 coding region into the pQE30 expression vector. However multiple bands were detected by Western analysis, probably due to the presence of rare codons. The RIG plasmid, which encodes tRNAs for rare codons in particular Arg (AGA/AGG), Ile (AUA) and Gly (GGA) was engineered into the E. coli strain resulting in production of full length PfHsp70. Purification was achieved through Ni²⁺ Chelating sepharose under denaturing conditions. PfHsp70 was found to have a very low basal ATPase activity of 0.262 ± 0.05 nmoles/min/mg of protein. In the presence of reduced and carboxymethylated lactalbumin (RCMLA) a 11-fold increase in ATPase activity was noted whereas in the presence of both RCMLA and Trypanosoma cruzi DnaJ (Tcj2) a 16-fold was achieved. For ATP hydrolysis kcat value of 0.003 min⁻¹ was obtained whereas for ADP release a greater kcat value of 0.8 min⁻¹ was obtained. These results indicated that rate of ATP hydrolysis maybe the rate-determining step in the ATPase cycle of PfHsp70.
- Full Text:
- Date Issued: 2004
Changes in energy use patterns in the Bushbuckridge Lowveld of the Limpopo Province, South Africa: eleven years on
- Authors: Madubansi, Mainza
- Date: 2004
- Subjects: Energy consumption -- South Africa -- Limpopo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4756 , http://hdl.handle.net/10962/d1007139 , Energy consumption -- South Africa -- Limpopo
- Description: This research reports on the energy transition that has taken place in the Bushbuckridge district between 1991 and 2002. It is a follow up to a similar study that was done in 1991 investigating a number of aspects of energy use. It uses the 1991 study as a baseline and aims to explore how the passage of time, growth of the local population and changes in incomes and the availability of fuels have affected the patterns of fuel use in the region in the past eleven years. A structured interview approach was used for most aspects of the study. The interview schedule included sections on types, amounts and reasons for use and non-use of 13 different energy sources as well as data on income levels, household size and other economic parameters. Analysis of the consumption patterns of the different fuel types revealed that between the two survey periods, households in the sample settlements went through some pronounced changes in patterns of fuel use, particularly those that had acquired electricity. The introduction of electricity in the region had certainly played a major role in spurring the energy transition. Fuels that were previously used for lighting, powering entertainment appliances and refrigeration had been displaced by electricity. In terms of cooking and other thermal application, however, the vast majority of households in all the sample settlements continued using fuelwood and complemented it to a lesser extent with paraffin and electricity. In both surveys, the use or non-use of available fuels in the region was influenced by several factors. Common reasons for non-use of certain fuel types included expense, lack of appliances, the risk involved in using such fuels and the preference for other fuels. Reasons for use were mainly related to the low cost of the fuel and the fuel’s ability to meet particular end uses. Although incomes in the region had increased between 1991 and 2002, they were still below the poverty line. Activities from which households obtained their income remained the same. Old age pension, migrant wages and the informal sector remained the largest contributors of income to most households. Other indicators of relative wealth and poverty, like ownership of vehicles, bicycles and beds remained the same.
- Full Text:
- Date Issued: 2004
- Authors: Madubansi, Mainza
- Date: 2004
- Subjects: Energy consumption -- South Africa -- Limpopo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4756 , http://hdl.handle.net/10962/d1007139 , Energy consumption -- South Africa -- Limpopo
- Description: This research reports on the energy transition that has taken place in the Bushbuckridge district between 1991 and 2002. It is a follow up to a similar study that was done in 1991 investigating a number of aspects of energy use. It uses the 1991 study as a baseline and aims to explore how the passage of time, growth of the local population and changes in incomes and the availability of fuels have affected the patterns of fuel use in the region in the past eleven years. A structured interview approach was used for most aspects of the study. The interview schedule included sections on types, amounts and reasons for use and non-use of 13 different energy sources as well as data on income levels, household size and other economic parameters. Analysis of the consumption patterns of the different fuel types revealed that between the two survey periods, households in the sample settlements went through some pronounced changes in patterns of fuel use, particularly those that had acquired electricity. The introduction of electricity in the region had certainly played a major role in spurring the energy transition. Fuels that were previously used for lighting, powering entertainment appliances and refrigeration had been displaced by electricity. In terms of cooking and other thermal application, however, the vast majority of households in all the sample settlements continued using fuelwood and complemented it to a lesser extent with paraffin and electricity. In both surveys, the use or non-use of available fuels in the region was influenced by several factors. Common reasons for non-use of certain fuel types included expense, lack of appliances, the risk involved in using such fuels and the preference for other fuels. Reasons for use were mainly related to the low cost of the fuel and the fuel’s ability to meet particular end uses. Although incomes in the region had increased between 1991 and 2002, they were still below the poverty line. Activities from which households obtained their income remained the same. Old age pension, migrant wages and the informal sector remained the largest contributors of income to most households. Other indicators of relative wealth and poverty, like ownership of vehicles, bicycles and beds remained the same.
- Full Text:
- Date Issued: 2004
Cleaning of fouled membranes using enzymes from a sulphidogenic bioreactor
- Authors: Melamane, Xolisa
- Date: 2004
- Subjects: Membrane filters , Membrane filters -- Fouling , Enzymes -- Biotechnology , Enzymes -- Purification , Water -- Purification -- Membrane filtration , Ultrafiltration
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4133 , http://hdl.handle.net/10962/d1015764
- Description: Maintenance of membrane performance requires inevitable cleaning or defouling of fouled membranes. Membrane cleaning using enzymes such as proteases, lipases, α-glucosidases from a sulphidogenic bioreactor was investigated. At first, dilute and concentrated enzyme extract were prepared form the sulphidogenic pellet. Enzyme assays on 0.5 % azocaisen, 1 % triacetin and 1 mg/ml ρ-nitrophenyl-α-D-glucopyranoside were performed using the concentrated enzyme extract (0 – 200 mg/ml). For membrane fouling, an abattoir effluent was obtained from Ostritech Pty (Ltd), Grahamstown, South Africa. The effluent was characterised for presence of potential foulants such as lipids, proteins, amino acids and carbohydrates. Static fouling of polysulphone membranes (0.22 μm, 47 mm) was then performed using the abattoir effluent. Cleaning of the fouled membranes was also performed using at first the dilute and then the concentrated form (200 mg/ml) of enzyme extracts. Qualitative and quantitative biochemical analysis for proteins, lipids and carbohydrates was performed to ascertain the presence of foulants on polysulphone membranes and their removal by dilute or concentrated enzyme extracts. The ability of dilute enzyme extracts to remove proteins lipids, and carbohydrates fouling capillary UF membrane module; their ability to restore permeate fluxes and transmembrane pressure after cleaning/defouling was also investigated. Permeate volumes from this UF membrane module were analysed for protein, amino acids, lipids, and carbohydrates concentrations after fouling and defouling. Fouling was further characterized by standard blocking, cake filtration and pore blocking models using stirred UF cell and polyethersulphone membranes with MWCO of 30 000, 100 000 and 300 000. After characterization of fouling, polyethersulphone membranes with MWCO of 30 000 and 300 000 were defouled using the concentrated enzyme extract (100 mg ml). Enzyme activities at 200 mg/ml of enzyme concentration were 8.071 IU, 86.71 IU and 789.02 IU for proteases, lipases and α-glucosidases. The abattoir effluent contained 553 μg/ml of lipid, 301 μg/ml of protein, 141 μg/ml of total carbohydrate, and 0.63 μg/ml of total reducing sugars. Proteins, lipids and carbohydrates fouling polysulphone membranes after a day were removed by 23.4 %, when a dilute enzyme was used. A concentrated enzyme extract of 200 mg/ml was able to remove proteins, lipids and carbohydrates up to 5 days of fouling by 100 %, 82 %, 71 %, 68 % and 76 % respectively. Defouling of dynamically fouled capillary ultrafiltration membranes using sulphidogenic proteases was successful at pH 10, 37°C, within 1 hour. Sulphidogenic proteases activity was 2.1 U/ml and flux Recovery (FR %) was 64. Characterization of fouling revealed that proteins and lipids were major foulants while low concentration of carbohydrates fouled polyethersulphone membranes. Fouling followed standard blocking for 10 minutes in all the membranes; afterwards fouling adopted cake filtration model for membranes with 30 000 MWCO and pore blocking model for membranes with 300 000 MWCO. A concentration of 100 mg/ml of enzyme extract was able to remove fouling from membranes with MWCO of 30 000. Defouling membranes that followed pore blocking model i.e. 300 000 MWCO was not successful due to a mass transfer problem. From the results of defouling of 30 000 and 300 000 MWCO it was concluded that defouling of cake layer fouling (30 000 MWCO) was successful while defouling of pore blocking fouling was unsuccessful due to a mass transfer problem. The ratio of enzymes present in the enzyme extract when calculated based on enzymatic activity for proteases, lipases and α-glucosidases was 1.1 %, 11 % and 87.9 %. It was hypothesized that apart from proteases, lipases, α and β-glucosidases; phosphatases, sulphatases, amonipeptidases etc. from a sulphidogenic bioreactor clean or defoul cake layer fouling by organic foulants and pore blocking fouling provided the mass transfer problem is solved. However, concentration of enzymes from a sulphidogenic bioreactor has not been optimized yet. Other methods of concentrating the enzyme extract can be investigated for example use of organic solvents.
- Full Text:
- Date Issued: 2004
- Authors: Melamane, Xolisa
- Date: 2004
- Subjects: Membrane filters , Membrane filters -- Fouling , Enzymes -- Biotechnology , Enzymes -- Purification , Water -- Purification -- Membrane filtration , Ultrafiltration
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4133 , http://hdl.handle.net/10962/d1015764
- Description: Maintenance of membrane performance requires inevitable cleaning or defouling of fouled membranes. Membrane cleaning using enzymes such as proteases, lipases, α-glucosidases from a sulphidogenic bioreactor was investigated. At first, dilute and concentrated enzyme extract were prepared form the sulphidogenic pellet. Enzyme assays on 0.5 % azocaisen, 1 % triacetin and 1 mg/ml ρ-nitrophenyl-α-D-glucopyranoside were performed using the concentrated enzyme extract (0 – 200 mg/ml). For membrane fouling, an abattoir effluent was obtained from Ostritech Pty (Ltd), Grahamstown, South Africa. The effluent was characterised for presence of potential foulants such as lipids, proteins, amino acids and carbohydrates. Static fouling of polysulphone membranes (0.22 μm, 47 mm) was then performed using the abattoir effluent. Cleaning of the fouled membranes was also performed using at first the dilute and then the concentrated form (200 mg/ml) of enzyme extracts. Qualitative and quantitative biochemical analysis for proteins, lipids and carbohydrates was performed to ascertain the presence of foulants on polysulphone membranes and their removal by dilute or concentrated enzyme extracts. The ability of dilute enzyme extracts to remove proteins lipids, and carbohydrates fouling capillary UF membrane module; their ability to restore permeate fluxes and transmembrane pressure after cleaning/defouling was also investigated. Permeate volumes from this UF membrane module were analysed for protein, amino acids, lipids, and carbohydrates concentrations after fouling and defouling. Fouling was further characterized by standard blocking, cake filtration and pore blocking models using stirred UF cell and polyethersulphone membranes with MWCO of 30 000, 100 000 and 300 000. After characterization of fouling, polyethersulphone membranes with MWCO of 30 000 and 300 000 were defouled using the concentrated enzyme extract (100 mg ml). Enzyme activities at 200 mg/ml of enzyme concentration were 8.071 IU, 86.71 IU and 789.02 IU for proteases, lipases and α-glucosidases. The abattoir effluent contained 553 μg/ml of lipid, 301 μg/ml of protein, 141 μg/ml of total carbohydrate, and 0.63 μg/ml of total reducing sugars. Proteins, lipids and carbohydrates fouling polysulphone membranes after a day were removed by 23.4 %, when a dilute enzyme was used. A concentrated enzyme extract of 200 mg/ml was able to remove proteins, lipids and carbohydrates up to 5 days of fouling by 100 %, 82 %, 71 %, 68 % and 76 % respectively. Defouling of dynamically fouled capillary ultrafiltration membranes using sulphidogenic proteases was successful at pH 10, 37°C, within 1 hour. Sulphidogenic proteases activity was 2.1 U/ml and flux Recovery (FR %) was 64. Characterization of fouling revealed that proteins and lipids were major foulants while low concentration of carbohydrates fouled polyethersulphone membranes. Fouling followed standard blocking for 10 minutes in all the membranes; afterwards fouling adopted cake filtration model for membranes with 30 000 MWCO and pore blocking model for membranes with 300 000 MWCO. A concentration of 100 mg/ml of enzyme extract was able to remove fouling from membranes with MWCO of 30 000. Defouling membranes that followed pore blocking model i.e. 300 000 MWCO was not successful due to a mass transfer problem. From the results of defouling of 30 000 and 300 000 MWCO it was concluded that defouling of cake layer fouling (30 000 MWCO) was successful while defouling of pore blocking fouling was unsuccessful due to a mass transfer problem. The ratio of enzymes present in the enzyme extract when calculated based on enzymatic activity for proteases, lipases and α-glucosidases was 1.1 %, 11 % and 87.9 %. It was hypothesized that apart from proteases, lipases, α and β-glucosidases; phosphatases, sulphatases, amonipeptidases etc. from a sulphidogenic bioreactor clean or defoul cake layer fouling by organic foulants and pore blocking fouling provided the mass transfer problem is solved. However, concentration of enzymes from a sulphidogenic bioreactor has not been optimized yet. Other methods of concentrating the enzyme extract can be investigated for example use of organic solvents.
- Full Text:
- Date Issued: 2004
CREWS : a Component-driven, Run-time Extensible Web Service framework
- Authors: Parry, Dominic Charles
- Date: 2004
- Subjects: Component software -- Development , Computer software -- Reusability , Software reengineering , Web services
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4628 , http://hdl.handle.net/10962/d1006501 , Component software -- Development , Computer software -- Reusability , Software reengineering , Web services
- Description: There has been an increased focus in recent years on the development of re-usable software, in the form of objects and software components. This increase, together with pressures from enterprises conducting transactions on the Web to support all business interactions on all scales, has encouraged research towards the development of easily reconfigurable and highly adaptable Web services. This work investigates the ability of Component-Based Software Development (CBSD) to produce such systems, and proposes a more manageable use of CBSD methodologies. Component-Driven Software Development (CDSD) is introduced to enable better component manageability. Current Web service technologies are also examined to determine their ability to support extensible Web services, and a dynamic Web service architecture is proposed. The work also describes the development of two proof-of-concept systems, DREW Chat and Hamilton Bank. DREW Chat and Hamilton Bank are implementations of Web services that support extension dynamically and at run-time. DREW Chat is implemented on the client side, where the user is given the ability to change the client as required. Hamilton Bank is a server-side implementation, which is run-time customisable by both the user and the party offering the service. In each case, a generic architecture is produced to support dynamic Web services. These architectures are combined to produce CREWS, a Component-driven Runtime Extensible Web Service solution that enables Web services to support the ever changing needs of enterprises. A discussion of similar work is presented, identifying the strengths and weaknesses of our architecture when compared to other solutions.
- Full Text:
- Date Issued: 2004
- Authors: Parry, Dominic Charles
- Date: 2004
- Subjects: Component software -- Development , Computer software -- Reusability , Software reengineering , Web services
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4628 , http://hdl.handle.net/10962/d1006501 , Component software -- Development , Computer software -- Reusability , Software reengineering , Web services
- Description: There has been an increased focus in recent years on the development of re-usable software, in the form of objects and software components. This increase, together with pressures from enterprises conducting transactions on the Web to support all business interactions on all scales, has encouraged research towards the development of easily reconfigurable and highly adaptable Web services. This work investigates the ability of Component-Based Software Development (CBSD) to produce such systems, and proposes a more manageable use of CBSD methodologies. Component-Driven Software Development (CDSD) is introduced to enable better component manageability. Current Web service technologies are also examined to determine their ability to support extensible Web services, and a dynamic Web service architecture is proposed. The work also describes the development of two proof-of-concept systems, DREW Chat and Hamilton Bank. DREW Chat and Hamilton Bank are implementations of Web services that support extension dynamically and at run-time. DREW Chat is implemented on the client side, where the user is given the ability to change the client as required. Hamilton Bank is a server-side implementation, which is run-time customisable by both the user and the party offering the service. In each case, a generic architecture is produced to support dynamic Web services. These architectures are combined to produce CREWS, a Component-driven Runtime Extensible Web Service solution that enables Web services to support the ever changing needs of enterprises. A discussion of similar work is presented, identifying the strengths and weaknesses of our architecture when compared to other solutions.
- Full Text:
- Date Issued: 2004
Design of a performance evaluation tool for multimedia databases with special reference to Oracle
- Authors: Stakemire, Tonia
- Date: 2004
- Subjects: Oracle (Computer file) , Application software -- Evaluation , Database management -- Evaluation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4705 , http://hdl.handle.net/10962/d1015975
- Description: Increased production and use of multimedia data has led to the development of a more advanced Database Management System (DBMS), like an Object Relational Database Management System (ORDBMS). These advanced databases are necessitated by the complexity in structure and the functionality required by multimedia data. Unfortunately, no suitable benchmarks exist with which to test the performance of databases when handling multimedia data. This thesis describes the design of a benchmark to measure the performance of basic functionality found in multimedia databases. The benchmark, called MORD (Multimedia Object Relational Databases), targets Oracle, a well known commercial Object Relational Database Management System (ORDBMS) that can handle multimedia data. Although MORD targets Oracle, it can easily be applied to other Multimedia Database Management System (MMDBMS) as a result of a design that stressed its portability, and simplicity. MORD consists of a database schema, test data, and code to simulate representative queries on multimedia databases. A number of experiments are described that validate MORD and ensure its correct design and that its objectives are met. A by-product of these experiments is an initial understanding of the performance of multimedia databases. The experiments show that with multimedia data the buffer cache should be at least large enough to hold the largest dataset, a bigger block size improves the performance, and turning off logging and caching for bulk loading improves the performance. MORD can be used to compare different ORDBMS or to assist in the configuration of a specific database.
- Full Text:
- Date Issued: 2004
- Authors: Stakemire, Tonia
- Date: 2004
- Subjects: Oracle (Computer file) , Application software -- Evaluation , Database management -- Evaluation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4705 , http://hdl.handle.net/10962/d1015975
- Description: Increased production and use of multimedia data has led to the development of a more advanced Database Management System (DBMS), like an Object Relational Database Management System (ORDBMS). These advanced databases are necessitated by the complexity in structure and the functionality required by multimedia data. Unfortunately, no suitable benchmarks exist with which to test the performance of databases when handling multimedia data. This thesis describes the design of a benchmark to measure the performance of basic functionality found in multimedia databases. The benchmark, called MORD (Multimedia Object Relational Databases), targets Oracle, a well known commercial Object Relational Database Management System (ORDBMS) that can handle multimedia data. Although MORD targets Oracle, it can easily be applied to other Multimedia Database Management System (MMDBMS) as a result of a design that stressed its portability, and simplicity. MORD consists of a database schema, test data, and code to simulate representative queries on multimedia databases. A number of experiments are described that validate MORD and ensure its correct design and that its objectives are met. A by-product of these experiments is an initial understanding of the performance of multimedia databases. The experiments show that with multimedia data the buffer cache should be at least large enough to hold the largest dataset, a bigger block size improves the performance, and turning off logging and caching for bulk loading improves the performance. MORD can be used to compare different ORDBMS or to assist in the configuration of a specific database.
- Full Text:
- Date Issued: 2004
DRUBIS : a distributed face-identification experimentation framework - design, implementation and performance issues
- Authors: Ndlangisa, Mboneli
- Date: 2004
- Subjects: Principal components analysis , Human face recognition (Computer science) , Image processing , Biometric identification
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4704 , http://hdl.handle.net/10962/d1015768
- Description: We report on the design, implementation and performance issues of the DRUBIS (Distributed Rhodes University Biometric Identification System) experimentation framework. The Principal Component Analysis (PCA) face-recognition approach is used as a case study. DRUBIS is a flexible experimentation framework, distributed over a number of modules that are easily pluggable and swappable, allowing for the easy construction of prototype systems. Web services are the logical means of distributing DRUBIS components and a number of prototype applications have been implemented from this framework. Different popular PCA face-recognition related experiments were used to evaluate our experimentation framework. We extract recognition performance measures from these experiments. In particular, we use the framework for a more indepth study of the suitability of the DFFS (Difference From Face Space) metric as a means for image classification in the area of race and gender determination.
- Full Text:
- Date Issued: 2004
- Authors: Ndlangisa, Mboneli
- Date: 2004
- Subjects: Principal components analysis , Human face recognition (Computer science) , Image processing , Biometric identification
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4704 , http://hdl.handle.net/10962/d1015768
- Description: We report on the design, implementation and performance issues of the DRUBIS (Distributed Rhodes University Biometric Identification System) experimentation framework. The Principal Component Analysis (PCA) face-recognition approach is used as a case study. DRUBIS is a flexible experimentation framework, distributed over a number of modules that are easily pluggable and swappable, allowing for the easy construction of prototype systems. Web services are the logical means of distributing DRUBIS components and a number of prototype applications have been implemented from this framework. Different popular PCA face-recognition related experiments were used to evaluate our experimentation framework. We extract recognition performance measures from these experiments. In particular, we use the framework for a more indepth study of the suitability of the DFFS (Difference From Face Space) metric as a means for image classification in the area of race and gender determination.
- Full Text:
- Date Issued: 2004