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Design, formulation and evaluation of liposomes co-loaded with human serum albumin and rifampicin

  • Authors: Bapolisi, Alain Murhimalika
  • Date: 2020
  • Subjects: Liposomes , Rifampin , Antitubercular agents , Serum albumin , Albumins , Tuberculosis -- Treatment
  • Language: English
  • Type: text , Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10962/163179 , vital:41016
  • Description: Tuberculosis (TB) is a devastating infectious disease caused by Mycobacterium tuberculosis and is the leading cause of death from a single infectious agent. The high morbidity and mortality rates of TB are partly due to factors such as the lengthy regimen (of 6–24 months), the development of drug resistance, and the pathogen location within the macrophages. These, with poor physiochemical properties of existing drugs hamper the effectiveness of the treatment despite the existence of potent antibiotics such as Rifampicin (Rif). Hydrophobicity plagues many drugs, including Rif, which are then particularly affected due to inherently poor intracellular availability. Novel drug delivery approaches are therefore needed in order to optimize the cytotoxic potential of said antitubercular drugs. To improve the bioavailability of hydrophobic drugs, numerous delivery strategies have been developed. Amongst these, the coordination of cytotoxic drugs to therapeutic proteins have shown some success for improved efficacy in the management of illnesses including infectious diseases. Of therapeutic proteins, Human Serum Albumin (HSA) is an attractive drug carrier with interesting benefits such as low immunogenicity, antioxidant properties and improving cellular uptake of drugs through HSA-specific binding sites which are expressed on most cells including macrophages, where M. tuberculosis often resides. Hence, combination of Rif to HSA (Rif-HSA) seems a promising approach for improved intracellular delivery of Rif. However, the in vivo stability of colloidal protein-based therapeutics is mostly challenging and an effective vehicle is needed to control the biological fate of such conjugates. Liposomes seem to be appropriate carriers for the Rif-HSA complex due to their reputable applicability for encapsulating diverse materials (i.e., hydrophobic and hydrophilic compounds or small and complex molecules) and preventing chemical and biological degradation of the cargo. Therefore, the main objective of this study was to simultaneously encapsulate Rif and HSA in liposomes, which, to the best of our knowledge, has not been done before. The dual liposomes (Rif-HSA-lip) were made by a modified “Reverse Phase Evaporation” method (REV), following a Design of Experiments (DOE) approach to determine which factors impact the formulation. In addition, liposomes were made from crude soybean lecithin (CSL), rather than expensive and highly purified lipids. iv The liposomes were fully characterised, and the encapsulation efficiency (î) was monitored using high-performance liquid chromatography (HPLC). The results were correlated with factors such as organic and aqueous phase composition, as well as the in vitro release profile of Rif. Transmission electron microscopy (TEM) results confirmed the formation of spherical dual liposomes nanoparticles of roughly 200 nm. Dynamic light scattering (DLS) and Zeta potential measurements showed a negative charge (<–45 mV) and with satisfactory polydispersity (PDI<0.5). HSA dramatically improved the aqueous solubility of Rif (from1.9 mg/ml in water to around 4.3 mg/ml in HSA 10% solution) mainly due to Rif-HSA hydrophobic interactions. This resulted in a good î of almost 60% for Rif, despite the presence of bulky HSA in the lipid bilayer. These details were confirmed using proton nuclear magnetic resonance (1H NMR) and Fourier-transform infrared spectroscopy (FTIR). Furthermore, energy dispersive X-ray (EDX) and DLS data suggested the presence of HSA poking out on the surface of liposomes, which is encouraging for potential targeted delivery in the future. The in vitro release studies also depicted a substantial improvement in the diffusion of Rif in dual liposomes versus free Rif, from 65% after 12 hours for free Rif to 95% after only 5 hours for Rif- HSA-lip. Finally, stability studies conducted over 30 days at room temperature, showed that the freeze-dried formulations of Rif-HSA-lip exhibited good shelf stability over liposomes with no HSA. This study represents an illustrative example of co-loading of antibiotics and proteins into liposomes, which could encourage further development of novel nanoparticulate tools for the effective management of both drug-susceptible and -resistant infectious diseases such as TB.
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Detecting the transnational space: metaphysical detecting in Kazuo Ishiguro’s When we were orphans, Michiel Heyns’s Lost ground, and Amitav Gosh’s The Calcutta chromosome

  • Authors: Botes, Niki
  • Date: 2020
  • Subjects: Literature and transnationalism , Detective and mystery stories -- History and criticism , Ishiguro, Kazuo, 1954- , Ishiguro, Kazuo, 1954- -- When we were orpahns , Heyns, Michiel , Heyns, Michiel -- Lost ground , Ghosh, Amitav, 1956- , Ghosh, Amitav, 1956- -- The Calcutta chromosome
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: http://hdl.handle.net/10962/140971 , vital:37933
  • Description: The transnational detectives in the three primary texts under scrutiny—Kazuo Ishiguro’s When We Were Orphans, Michiel Heyns’s Lost Ground, and Amitav Ghosh’s The Calcutta Chromosome—are regarded as metaphysical detectives in this thesis. As transnational figures, they criss-cross physical and national borders, but their investigations also often require the crossing of epistemological and ontological borders, as well as thinking outside of binaries. The fictional transnational, postcolonial worlds presented in these texts all feature a lack of change and the persistence of the socio-economic inequalities and discriminations of the colonial or, in the case of Lost Ground, the apartheid past. This thesis will argue that the three texts foreground the inability to break free from conceptual frameworks and a lack in human relationships as two possible reasons why the communities and societies in question have not been able to affect change. To solve the various mysteries in the texts, the transnational detectives need to find new ways of knowing and being. The lessons that these detectives learn and the personal changes they experience serve as synecdoche for how a better tomorrow for the transnational, postcolonial world could possibly be achieved. The methodology employed in this thesis involves a comparative reading of the three primary texts. To analyse and interpret these texts, this thesis draws on the philosophies of postmodern philosophers such as Martin Heidegger, Michel Foucault, Jacques Derrida, and Emmanuel Levinas, as well as postcolonial theorists like Edward Said, Gayatri Chakravorty Spivak, and Homi K. Bhabha. The thesis will contend that especially Derrida’s work on deconstruction, which forms the conceptual basis for Bhabha’s notion of the ‘third space,’ as well as Levinas’s anti-totalitarian approach with regard to the ethical relationship between the self and the other, are viable better ways of knowing and being in the transnational, postcolonial worlds presented in the texts. Derrida’s notion of différance and the trace, as well as Bhabha’s third space, this thesis contends, completely destabilises binary thinking and offers ways to think outside of the limited frameworks of ideological discourse. This thesis also posits that Levinas’s metaphysical and pluralistic interpretation of how the self should, and can, relate to the other, presents a better way of forming human relationships and has the potential to affect positive change.
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Developing an organisational culture in support of organisational capacity for change: the case of a South African bank

  • Authors: Dhoba, Howard
  • Date: 2020
  • Subjects: Corporate culture , Bank management , Organizational change
  • Language: English
  • Type: text , Thesis , Masters , MBA
  • Identifier: http://hdl.handle.net/10962/141247 , vital:37956
  • Description: Business organisations are increasingly being exposed to challenging environmental pressures. It has been widely acknowledged and observed that an organisation that crafts an effective organisational culture should be well positioned to react to these threatening changes, and to proactively seize opportunities to adapt and learn. This research study seeks to investigate how an organisational culture that supports organisational capacity for change can be developed. It analyses what a banking organisation’s espoused culture is made up of and how it was formulated. A thematic analysis of how the bank’s aspirational and innovative culture contributes to the organisation’s capacity for change is also presented. Organisational management should take a leading role in developing the values and practices that foster participation, which are then imparted to the entire organisation. The research study thus only focused on analysing the development of organisational culture from the management perspective. An interpretivist research approach was adopted, with a case study design as the research method. Data was gathered through conducting personal interviews with twelve managers from a Retail and Business Banking cluster of one of the big South African banks. Numerous interventions were employed in the organisation to develop espoused values, aspirational culture and to encourage all employees to champion innovations and constantly search for better ways to offer delighting value propositions to clients. The research findings can assist managers and organisational leaders in building their organisational capacity for change through developing an effective organisational culture that supports change initiatives. Since the research study only focused on analysing the development of organisational culture from the perspective of management, further research studies may involve employees as well, due to the fact that everyone is expected to take an active role for an organisation to display the overall capability to effectively respond to a progressively volatile environment. The research was able to reveal how key initiatives that are designed from theoretical constructs can be used to deliver on an innovative organisational culture that contributes to organisational capability for change.
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Developing social indicators for the evaluation of natural resource management programmes using a capability approach in the Eastern Cape, South Africa

  • Authors: Human, Johanna Susanna
  • Date: 2020
  • Subjects: Environmental education -- South Africa -- Eastern Cape , Natural resources -- Management -- Study and teaching (Continuing education) -- South Africa -- Eastern Cape , Sustainable development -- Study and teaching (Continuing education) -- South Africa -- Eastern Cape , Environmental economics -- Study and teaching (Continuing education) -- South Africa -- Eastern Cape , Subsistence farming -- South Africa -- Eastern Cape , Watersheds -- South Africa -- Eastern Cape , Capabilities approach (Social sciences) , Tsitsa River Catchment (Eastern Cape, South Africa)
  • Language: English
  • Type: Thesis , Masters , MEd
  • Identifier: http://hdl.handle.net/10962/147348 , vital:38628
  • Description: The dissertation investigates how the capability approach can be used to develop social indicators for a natural resource management (NRM) programme. The study aimed to develop an understanding of what the beneficiaries of the programme value being and doing to guide the development of social indicators that will take into consideration their personal development aspirations. These valued beings and doings were then overlaid with the programme goals and objectives to identify and select the final indicators. The study was situated in the Tsitsa catchment in the Eastern Cape, South Africa and was part of a natural resource management programme, focused on land restoration and avoided degradation. Methods used to collect data for this study and develop an understanding of what the residents’ valued beings and doings were, included semi-structured interviews, workshops and document analysis. The research population was non-homogenous. I tried to include voices representing different prominent groups of land users in the study. This included established commercial farmers, emerging commercial farmers, commercial forestry, residents residing in communal areas and engaged with subsistence farming and residents from these communal areas who are now residing elsewhere but continue to have connections to the catchment. Understanding the voices of these different groups who reside in the catchment was important to develop an inclusive understanding of what residents of the catchment valued beings and doings were. The research process was supported by participatory approaches. This process, and the lessons learnt during the research process, were valuable for the further development and implementation of the planned participatory monitoring evaluation reflection and learning (PMERL) framework. The research process involved learning from the research participants about their valued beings and doings and what was important for the programme implementers. Some of the research participants from the Rhodes University implementation team (RUIT) were also involved in selecting the indicators they thought the programme should monitor. Overall, using the capability approach helped me to gain insight and understanding with regard to what the valued beings and doings are for the residents of the catchment and what they would like development programmes, such as the Tsitsa Project, to address. It was surprising to find many areas of overlap between the valued beings and doings of the different residents in the catchment, as well as the similarities between these and the goals and objectives of the Tsitsa Project. In conclusion, using the capability approach was useful to learn what was important for the residents of the catchment to help develop the social indicators but also to learn how to guide and steer participatory monitoring and evaluation processes in the future.
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Developing voice and leadership amongst a group of class captains

  • Authors: Nehunga, Jacobina Taukondjele
  • Date: 2020
  • Subjects: Educational leadership -- Namibia , Transformational leadership -- Namibia , Leadership in adolescents -- Namibia , Rural schools -- Namibia , School children – Namibia -- Attitudes , School management and organization – Namibia , Cultural Historical Activity Theory
  • Language: English
  • Type: Thesis , Masters , MEd
  • Identifier: http://hdl.handle.net/10962/145034 , vital:38402
  • Description: The notion that leadership can be reduced to that of a formal position such as principalship has become problematic in school leadership research and practice, globally and also in Namibia. It appears that when leadership is concentrated only at the level of a principal within schools, teachers and learners within the same school experience a lack of opportunities to contribute to leadership practices, thus losing a sense of ownership. Supporting a distributed leadership perspective and embracing transformative leadership, this study investigated how learner voice and learner leadership (see for example Mitra and Gross, 2009) can be developed within a group of class captains in a combined school in rural Namibia. Cultural Historical Activity Theory (CHAT) underpinned the study which is located within a critical paradigm. Findings of the study were captured into two phases whereby phase one generated contextual profiling findings through the adoption of qualitative methods such as questionnaires, interviews, document analysis and two other participatory methods of mapping and transect walks. Phase two findings were captured through expansive learning actions in Change Laboratory Workshops. Observation notes, video recording and photographs and informal discussions were used as data sources during this phase. Both inductive and abductive analysis were employed. The findings revealed that a few enabling conditions to class captainship practice existed, but generally there was limited participation of class captains in school leadership. Class captains were seen as merely class representatives and their leadership was confined to a classroom level in the school. They performed more managerial tasks than leadership. It was also revealed that class captainship is not a documented practice in Namibia and it lacks support. Against this backdrop, during phase two of the study, seven contradictions were identified and two contradictions were prioritised for resolution during the Change Laboratory Workshops. Some of the resolutions included the development of a school based guiding document for class captains; an establishment of a class captainship club; and the promotion of a yearly election campaign of class captains. The study recommends more interventionist studies to be conducted on class captainship in order to see the practice documented in terms of policy in Namibia, perhaps in the near future.
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Development of graphene materials and phthalocyanines for application in dye-sensitized solar cells

  • Authors: Chindeka, Francis
  • Date: 2020
  • Subjects: Dye-sensitized solar cells , Graphene , Phthalocyanines , Molecular orbitals , Impedance spectroscopy
  • Language: English
  • Type: text , Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10962/166092 , vital:41328
  • Description: Two sets of dye-sensitized solar cells (DSSCs) were fabricated. In the first set, dye-sensitized solar cells (DSSC) were fabricated by incorporating graphene materials as catalysts at the counter electrode. Platinum was also used as a catalyst for comparative purposes. Different phthalocyanines: hydroxyl indium tetracarboxyphenoxy phthalocyanine (1), chloro indium octacarboxy phthalocyanine (2) and dibenzoic acid silicon phthalocyanine (3) were used as dyes. Complex 3 gave the highest power conversion efficiency (η) of 3.19% when using nitrogen doped reduced graphene oxide nanosheets (NrGONS) as a catalyst at the counter electrode, and TiO2 containing rGONS at the anode. The value obtained is close to 3.8% obtained when using Pt catalyst instead of NrGONS at the cathode, thus confirming that NrGONS is a promising candidate to replace the more expensive Pt. The study also shows that placing rGONS on both the anode and cathode improves efficiency. In the second set, DSSCs were fabricated by using 2(3,5-biscarboxyphenoxy), 9(10), 16(17), 23(24)-tri(tertbutyl) phthalocyaninato Cu (4) and Zn (5) complexes as dyes on the ITO-TiO2 photoanodes containing reduced graphene oxide nanosheets (rGONS) or nitrogen-doped rGONS (NrGONS). The evaluation of the assembled DSSCs revealed that using ITO-TiO2-NrGONS-CuPc (4) photoanode had the highest fill factor (FF) and power conversion efficiency (ɳ) of 69 % and 4.36 % respectively. These results show that the asymmetrical phthalocyanine complexes (4) and (5) showed significant improvement on the performance of the DSSC compared to previous work on symmetrical carboxylated phthalocyanines with ɳ = 3.19%.
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Digital media marketing and the consumption of sugar-sweetened beverages in Africa: A reception analysis of the multi-channel marketing of Coca-Cola among young Africans from the University of Lagos, Nigeria and Rhodes University, South Africa

  • Authors: Akingbade, Olutobi Elijah
  • Date: 2020
  • Subjects: Carbonated beverages -- Health aspects -- South Africa , Carbonated beverages -- Marketing -- South Africa , Soft drinks -- Health aspects -- South Africa , Soft drinks -- Marketing -- South Africa , Carbonated beverages -- Health aspects -- Nigeria , Carbonated beverages -- Marketing -- Nigeria , Digital media -- Marketing -- Africa , Soft drinks -- Health aspects -- Nigeria , Soft drinks -- Marketing -- Nigeria , Digital media -- Marketing -- Nigeria , Obesity -- Africa , Nutritionally induced diseases -- Africa , Coca-Cola Company -- Marketing , Rhodes University -- Students -- Attitudes , University of Lagos -- Students -- Attitudes
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: http://hdl.handle.net/10962/163125 , vital:41012
  • Description: This study investigates and examines how Coca-Cola’s marketing communications, especially the newer forms of digital, social and mobile media marketing messages/campaigns, are received, understood and made sense of by two sets of purposefully selected young urban African students in Nigeria and South Africa. Embedded within a qualitative research design and underpinned by an interpretative phenomenological analysis (IPA) approach, this study was conducted against the backdrop of the recent surge in the consumption of Sugar Sweetened Beverages (SSBs) which has been directly implicated in the rise of obesity and a variety of non-communicable diseases (NCDs) in Africa. The thesis explores the role of multinational SSBs in this surge, as African countries have become key focus areas for multinational food and beverage companies seeking growth and profits, as home markets decline partly due to better health communications and, in some cases, the implementation of so-called ‘sugar taxes’ and the attendant negative publicity around these taxes. The focus on young Africans from Nigeria and South Africa was motivated by the similar rapid urbanisations in both countries, often accompanied by changes in diet and greater consumption of fast foods and SSBS, and by South Africa’s ranking as the country with the highest prevalence of overweight persons and obesity in Sub-Saharan Africa. Similar rises in national average weights are now also starting to be seen in Nigeria, as are surges of diet-related disease incidence and prevalence. The study is informed by Consumer Culture Theory (CCT) but also draws on other theories and some key concepts from marketing studies, health science and psychology. Methodologically, the study draws on in-person observations, focus group interviews and semi-structured individual in-depth interviews, to explore how Coca-Cola has created a deep and evocative historical ‘brandscape’ and how it has become a multicultural resource in both South Africa and Nigeria. Through an investigation into the lived experiences of study participants with regards to both their earliest and more recent engagements with Coca-Cola, as a brand and as a product, the study delineates the influence of older generations of Coke enthusiasts and consumers within participants’ households and newer spaces of interaction with Coke via interactive, highly personalised social media-centric campaigns. This study explores how the ubiquitous nature of Coca-Cola’s aesthetics and signage are engaged with – often in very ‘sub-conscious’ ways – by these students and how more recent social media campaigns evoke this multigenerational history. Unpacking study participants’ self-understandings of Coke and their often ‘sub-conscious’ engagements with the SSB, this study explicates the underpinning ideological grounding and how this is sustained over time to become an hegemonic code that does not only confine participants’ engagements with SSBs to Coke but also confines their reception of, and engagements with, Coke’s media marketing messages/campaigns to those that resonate with the multigenerational history evoked by the SSB. It is within this contextual background that this study brings to the fore participants ‘cognitive dissonance’ and scepticism and often rank disbelief of the health risks posed by their high levels of Coke consumption. The study concludes that attempts to raise awareness about the dangers inherent in excessive consumption of SSBs in Africa need to be reviewed and rethought. There is a need for long-term, consistent and much more proactive health journalism, alongside public health campaigns in both official and indigenous languages, to dispel the powerful myths created by SSB marketing and explain how SSBs are implicated in the rise of diet-related NCDs in Nigeria and South Africa.
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Disco

Discursive constructions of alcohol use and pregnancy among participants in intervention aimed at reducing Foetal Alcohol Spectrum Disorders

  • Authors: Msomi, Nqobile Nomonde
  • Date: 2020
  • Subjects: Fetal alcohol spectrum disorders -- South Africa , Pregnancy -- Psychological aspects , Reproductive rights -- South Africa , Reproductive health -- South Africa
  • Language: English
  • Type: text , Thesis , Masters , MA
  • Identifier: http://hdl.handle.net/10962/140374 , vital:37883
  • Description: South Africa’s socio-cultural and political history has had significant effects on maternal and reproductive health. The hazardous alcohol use patterns in the country have affected alcohol consumption during pregnancy. Antenatal exposure to alcohol may result in Foetal Alcohol Spectrum Disorders (FASD). The levels of FASD in particular areas of the country are the highest recorded in the world. Epidemiological studies have dominated pregnancy and FASD research in South Africa; however, recently critical scholarship seeking to contextualise the issue of drinking alcohol during pregnancy is emerging. This study forms part of a developmental/formative assessment of an alcohol and pregnancy intervention. Assessment is an important part of pilot interventions, and discourse is a key area of focus due to its constitutive role for the subjectivity of human beings and legitimation of institutional practices. Using a reproductive justice perspective and a Foucauldian approach to analysis, I identified five prominent discursive constructions of alcohol use during pregnancy produced during interviews with community educators. These interviews were conducted following training workshops with the community educators. Participants constructed their living environments as ‘wholly bad’ and ‘issue-ridden’ and positioned alcohol consumption as ‘a destroyer!’, ‘king’ and a social lubricant. They interpellated the foetus, the ‘FASD child’ and pregnant women into this context. They positioned themselves as transformed subjects able to effect change. The foetus was constructed as ‘vulnerable and important’, as opposed to the ‘defiled FASD child’. Pregnant women were constructed as ‘ignorant, preoccupied and unreceptive to knowledge’. These constructions hinged on so-called ‘scientific knowledge’ of biological processes in utero, demonstrating Foucault’s conception of the power/knowledge nexus and how its dynamics transforms knowledge of human beings. Whereas this ‘knowledge’ transformed alcohol consumption and the foetus into powerful and vulnerable subjects respectively, the circulating discourses had objectivising effects on pregnant women. The discourses of responsibilisation, the personification of the foetus, ‘the problem’ category of FASD, the discourse of difference, and the discourse of alcohol consumption as an entrenched practice were circulating around pregnant women. I suggest alterations to the identified constructions using principles of community psychology, the harm reduction model, a social model of disability and the reproductive justice perspective
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Distribution, habitat associations and conservation of the Eastern Cape rocky Sandelia bainsii (Perciform: Anabantidae) in the Great Fish River system

  • Authors: Sifundza, Delsy Sindy
  • Date: 2020
  • Subjects: Anabantidae -- South Africa -- Great Fish River , Perciformes -- South Africa -- Great Fish River , Labyrinth fishes -- South Africa -- Great Fish River , Fishes -- Effect of human beings on -- South Africa -- Great Fish River , Fish populations -- South Africa -- Great Fish River , Fish declines -- South Africa -- Great Fish River , Fish populations -- Monitoring -- South Africa -- Great Fish River , Anabantidae -- Evolution -- South Africa -- Great Fish River , Gene flow , Mitochondrial DNA , Microsatellites (Genetics) , Eastern Cape rocky Sandelia bainsii , Freshwater ecology -- South Africa -- Great Fish River
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10962/142692 , vital:38102
  • Description: In South Africa, freshwater ecosystems are under pressure due to increasing anthropogenic factors degrading river systems. The Cape Fold, the lower Southern Temperate Highveld and the Amatolo – Winterberg Highlands freshwater ecoregions contain some of the heavily impacted river systems in the country. One such river system is the Great Fish River system, which has been affected by anthropogenic modification (hydrological modification, water pollution and invasion by non-native fish species). These modifications have raised conservation concerns on an imperilled anabantid fish, the Eastern Cape rocky Sandelia bainsii that is confined to the Kat and Koonap rivers, which are tributaries of the Great Fish River. Historically, the species was reportedly common and abundant, with a range spanning across seven river systems (Kowie, Great Fish, Keiskamma, Igoda, Gxulu, Buffalo and Nahoon rivers) in the Eastern Cape Province. However, surveys have indicated that the species has experienced decline in population size and distribution range, with localised extirpations being reported. This resulted in its listing on the IUCN Red List of threatened species as Endangered. The proposed shale gas exploration and potential infrastructure development in the Karoo basin, which encompasses the headwaters of the Kat River, represent a potential future threat to the remnant populations of this species. The aim of the present study was to review the past and present distribution range of S. bainsii, determine the habitat associations and assess the conservation of S. bainsii in the Great Fish River system. This information is essential for informing conservation and management decisions for this species in the Great Fish River system. This study undertook a comprehensive survey of the Kat and the Koonap rivers in 2017, and augmented this information with data from more recent surveys that were conducted from 2009 - 2014 to map the distributions freshwater fishes in the Great Fish River system. Data from these recent surveys (2009 – 2017) were compared with historical records (1961 – 2005) obtained from the South African Institute for Aquatic Biodiversity to evaluate changes in distribution patterns of S. bainsii. Historical records indicated that S. bainsii occurred at 11 localities in the Kat River and three localities in the Koonap River. Data from recent surveys indicated that the species has persisted at these historical localities, except one locality in the Koonap River where no individuals of S. bainsii were captured, despite the use of multiple sampling approaches and gears. Although the species still persists at two of the three historical localities in the Koonap River, the population sizes have considerably declined as the species was reportedly in high abundance at these localities in the 1980s. Factors threatening the continued existence of this species in the Koonap River likely include non-native species, extreme drought conditions and the associated excessive abstraction of water which has resulted in the drying of some of the refugia pools where the species was historically common and abundant. In contrast to the Koonap River, the 2017 survey indicated that in the Kat River, S. bainsii had a similar distribution range compared to historical records. The species occurred at 11 of the 41 localities that were sampled in the Kat River, and the sampled populations consisted of all size classes. Sandelia bainsii occurred with other native fish species in the system, including Labeo umbratus, Enteromius anoplus and Glossogobius callidus. A comparison of historical and recent data indicates that non-native fishes have likely not expanded their ranges in the Kat River. This is likely due to the presence of weirs and dams that have prevented upstream movement of non-native fish species L. aeneus, C. gariepinus and T. sparrmanii which are now abundant in the lower Kat and mainstem Great Fish River. Surveys from the present study indicated that although S. bainsii still persists within the Kat River, there are a number of factors that pose a considerable threat to the continued existence of this species in the Great Fish River system. Chapter 3 evaluated the habitat associations of S. bainsii based on 10 physical and chemical variables and distribution data collected from 30 localities in the Upper Kat River and seven of its tributaries. A non-metric multidimensional scaling model was used to assess the distribution patterns of S. bainsii in relation to environmental variables. Furthermore, a negative binomial generalised linear model (GLM) was used to assess the relationship between the abundance of S. bainsii and environmental variables. Results from the present study indicated that S. bainsii was a habitat generalist as the distribution of this species showed no specific association with any of the habitat variables that were considered in this study. The species occurred in a wide range of habitats, including rocky sections in the upper Kat River and its tributaries as well as the mainstem sections. The GLM however indicated that the abundance of S. bainsii was negatively associated with increasing conductivity. In addition, the Kat River contains multiple weirs of variable sizes. Future studies should therefore evaluate the effects of these weirs on population fragmentation, gene flow and long-term evolutionary potential of S. bainsii through the application of techniques such as mitochondrial DNA and microsatellites.
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Diteng tsa ditlhopha tsa maina a Bantu: ntlhathakanelo e le mo Setswanang : “The semantics of Bandu noun classes: a focus on Setswana

  • Authors: Tladi, Oboitshepo
  • Date: 2020
  • Subjects: Tswana language , Tswana language -- Grammar , Tswana language -- Noun , Noun , Bantu languages , Bantu languages -- Noun , Bantu languages -- Grammar
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: http://hdl.handle.net/10962/167654 , vital:41500
  • Description: The present study investigated the semantic classification of the Setswana noun class system. This enquiry falls under the broad area of the noun classification system in Bantu languages, psycholinguistics and lexicogrpahy. Specifically it explores the basis of noun classification in Setswana making indications that Setswana noun classification is based on a partial semantic classification. Data for the study was drawn from the Setswana Oxford Dictionary. Sixty Setswana nouns, from class 1, 3, 5, and 7, were selected and analysed and then grouped into semantic categories (i.e., PERSON, DEROGATION, TRANSPORATION and so forth). The study adopted Kgukutli’s (1994) semantic classification in performing the dictionary analysis. The rest of the data was drawn from the intuitions of thirty-nine contemporary speakers of Setswana, with the aid of a linguistic test which was fashioned according to Selvik’s (2001) psycholinguistic test. The language test required participants to match the predetermined Setswana definitions with hypothetical Setswana nouns with selected class prefixes attached to them. The results from the empirical study showed that speakers were associating prefixes to certain semantic values, suggesting that each noun class had specific semantic content that was unique to that class. The semantic categories created through the dictionary analysis were then compared to those given by the thirty-nine Setswana speakers, to analyse whether there were any similaritires in the semantic classification of the noun classes. The findings of the dictionary analysis and linguistic test revealed that there were certain semantic characteristics that each class was associated with that seemed to be unique to the class. However, there were various semantic overlaps in the semantic categories associated with the different noun classes, which brings into question whether a semantic classification is viable in the classing of nouns. The study suggests that prior classification of Setswana nouns are not precise enough and that additional semantic categories are needed to offer a more precise classification of nouns in this language.
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Do submarine canyons influence the structure and diversity of benthic fish assemblages on the continental shelf edge?

  • Authors: Nyawo, Mpilonhle Aura June-Rose
  • Date: 2020
  • Subjects: Submarine valleys , iSimangaliso Wetland Park (South Africa) , Biotic communities -- South Africa -- iSimangaliso Wetland Park , Groundfishes -- South Africa -- iSimangaliso Wetland Park , Marine ecology -- South Africa -- iSimangaliso Wetland Park
  • Language: English
  • Type: text , Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10962/167264 , vital:41462
  • Description: Marine ecosystems play an important role in human welfare, such as providing food security and providing social, economic and environmental benefits to an increasing human population. However, due to the deleterious impact of overfishing as well as habitat degradation, pollution and climate change, many marine ecosystems and the substantial biodiversity they support, are under increasing threats. Submarine canyon ecosystems cut into the continental margin in all our oceans and are considered regions of enhanced diversity, abundance and biomass for many marine communities, including fish. In South Africa, the tropical Delagoa Ecoregion is characterised by numerous submarine canyons that are home to coelacanths Latimeria chalumnae, and a variety of commercially important line fish species. However, there is currently a lack of information on the ecological structure and functioning of these canyons, in relation to nearby non-canyon ecosystems. The aim of this thesis was to generate knowledge on the role of submarine canyons in influencing the benthic fish assemblage structure and diversity on the continental shelf and shelf edge within the high-latitude coral reef ecosystems in the tropical Delagoa Ecoregion of South Africa. First, a systematic literature review was conducted to examine the current knowledge on the role of submarine canyons on the benthic fish assemblages occurring on continental margins, as well as outline the trends in canyon research. For the fieldwork component of this thesis, baited remote underwater stereo-video systems were utilised to gather data. The first research chapter, chapter 3, investigated the effect of ecosystem and depth on the diversity and structure of benthic fish assemblages within iSimangaliso Wetland Park in the Delagoa Ecoregion. Data were collected in 2017 from Wright Canyon (canyon ecosystem) and adjacent slope ecosystems around the canyon (non-canyon ecosystem) at depths ranging between 70–240 m. The second research chapter, chapter 4, data were collected in 2018, from 60–100 m depths in three ubmarine canyons (Wright, Diepgat,Levens) and their adjacent habitat within iSimangaliso Wetland Park to investigate the diversity patterns and community structure of benthic fish and cons ider location as a factor. The systematic review showed that canyon research is still very much in its infancy, however more papers are expected to be published in the future. There were clear location associated biases detected, with more than 90% of the canyon research being concentrated in the Mediterranean Sea, while only one study was published from South Africa. Most of the reviewed literature focused on invertebrates, with benthic fish (including cartilaginous fish) being the second most studied taxa. A wide range of methods from both fishery- dependent and fishery-independent categories were used to gather the data of benthic fish from the canyons and adjacent areas. With over 8500 submarine canyons mapped globally, the number of submarine canyon research published and the detected location bias is of great concern. More studies are needed in order to understand the role of submarine canyons on adjacent slope habitats Overall, the results from the research chapters indicated that there were higher a bundances in the canyon ecosystem compared to the non-canyon ecosystem. In contrast, evenness and beta-diversity were higher in the non-canyon ecosystem. Relief, bottom type as well as depth played a major role in explaining the observed abundance and diversity patterns. Both evenness and beta-diversity decreased with increase in depth. The canyon and non-canyon also differed in terms of habitat structure. The canyon ecosystem was characterised by patched reef and reef habitats with high relief whereas the non-canyon ecosystem was characterised by flats and habitats, especially at shallower depths. Additionally, there were clear differences in terms of the fish community structure between the canyon and the non-canyon ecosystem. The heterogeneous and complex habitats of the canyon ecosystem were typified by species such as Epinephelus poecilonotus, Polysteganus coeruleopunctatus and Chrysoblephus puniceus in comparison to the less complex habitats outside the canyons that were preferred by species such as Lagocephalus sceleratus, and Rhinobatos leucospilus. The different locations sampled demonstrated that the canyon effect was not onsistent at all locations, with varying results detected in the different sample locations. This research demonstrates that clea r differences exists between the benthic fish assemblages associated with canyon and non-canyon ecosystems and provides important information on the role of submarine canyons in the iSimangaliso Wetland Park. From this thesis, it is clear that a ‘canyon effect’ exists in the continental shelf and shelf edge. The results suggested that heterogeneity of habitats was the most important environmental factor that differed between the canyon and non-canyon ecosystems and these differences in the habitat were driving the observed patterns. This thesis has provided a reference point, with regards to investigating the role and influence of these canyons on benthic fish assemblages occurring on the continental shelf edge. The information provided by this thesis can ultimately be used in other studies investigating benthic fish assemblages in other submarine canyons within iSimangaliso Wetland Park and the rest of South Africa./
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Does the construction of ‘rapeable’ bodies constitute an instance of hermeneutical injustice?

  • Authors: Weiffenbach, Michaela
  • Date: 2020
  • Subjects: Zuma, Jacob -- Trials, litigation, etc. , Rape -- Philosophy , Women's rights -- Africa , Women, Black -- Social conditions -- Africa , Masculinity -- Africa , Men, Black -- Africa -- Psychology , Justice (Philosophy)
  • Language: English
  • Type: text , Thesis , Masters , MA
  • Identifier: http://hdl.handle.net/10962/145708 , vital:38460
  • Description: This thesis argues that within the conventional imagination of the West, identity functions in a way that particular forms of embodiment are characterised by the experience of endured sexual vulnerability, which I argue is best understood as the construction of ‘rapeable’ bodies (Cahill 2001, 120). By this, I mean that the threat of rape is contingent upon the social and political construction of one’s identity as vulnerable. In virtue of this particular way of constructing the embodiment of certain subjectivities, there is an instance of hermeneutical injustice conferred (Fricker 2007, 114). I inquire into the function and meaning of stereotypic generalisations, prejudice and rape myths embedded within the dominant framework of the West and show how language and representation constructs these identities as ‘rapeable’. Furthermore, I consider how collective hermeneutical discourses construct Blackness, specifically Black masculinities and the construction of the ‘un-rapeable’ Black femxle body. To see how these ideas are congruous the prominent example that occurred in South Africa in 2005, namely, the Jacob Zuma rape trial comes to mind. One relevant feature of the case is that it shows how identities of race are constructed in the aftermath of apartheid and points to a Western collective imagination resistant to change. In addition, it demonstrates the triad of interrelatedness holding between the self-world-other, a relationship constituted mutually by the self and the socially constructed interpretations of identity and embodiment (du Toit 2009, 58). Lastly, the construction of ‘rapeable’ bodies is an example of how discursive narratives construct particular identities as vulnerable through rendering particular embodied subjects as sexually irrelevant and hermeneutically obscured.
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Ecological engineering: an assessment of the ecological impact of Reno mattress structures used in erosion control in the Keurbooms Estuary, South Africa

  • Authors: De Villiers, Nina
  • Date: 2020
  • Subjects: Sediments (Geology) -- Management , Sediments (Geology) -- South Africa -- Keurboomstrand , Coast changes -- South Africa -- Keurboomstrand , Shore protection -- South Africa -- Keurboomstrand , Coastal engineering-- South Africa -- Keurboomstrand , Coastal zone management-- South Africa -- Keurboomstrand , Estuarine ecology-- South Africa -- Keurboomstrand , Eelgrass -- South Africa -- Keurboomstrand , Reno Mattresses
  • Language: English
  • Type: text , Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10962/166138 , vital:41332
  • Description: Global climate changes have been associated with ocean warming and sea-level rise. Armouring of coastlines has become common practice with the increasing threat of coastal erosion. The transformation of soft sediment habitats to hard, artificial habitats because of coastline armouring can lead to changes in species diversity, composition and distribution. It is, therefore, essential to assess changes to habitats from coastal development as well as the ecological impact erosion control structures have within coastal systems. Ecological engineering attempts to combine engineering principals and ecological processes to reduce environmental impacts from coastal development and the implementation of artificial structures. Estuaries are particularly vulnerable to anthropogenic impacts through development, and are extremely important systems offering nursery and foraging grounds for many species. These systems are, however, particularly vulnerable to anthropogenic impacts from urbanisation and development. Within South Africa many estuaries are being transformed by the addition of artificial structures to combat erosion, one such structure is the Reno mattress (a flattened wire box filled with rocks). This study compared the fish diversity and abundances of existing Reno mattress structures and natural eelgrass (Zostera capensis) habitat in the Keurbooms Estuary, South Africa. Benthic invertebrates were sampled using standard core sampling and an adapted suction sampling approach within the two habitats. The non-destructive method of mini Baited Remote Underwater Video Systems (BRUVs) was used to sample fish. Seasonal benthic invertebrate and fish abundances and assemblages were assessed from winter 2018 to spring 2019 with greater abundances of both recorded in summer. Significantly greater abundances, diversity, and richness of fish were found in the Reno mattress habitat compared to Z. capensis. Invertebrate taxa displayed some overlap between habitats, however, three higher taxonomic groups were only recorded within Reno mattress habitat and one only within the eelgrass habitat. Fish assemblages differed significantly between the two established habitats. A Before-After-Control-Impact (BACI) investigation was used to assess the ecological impact of newly installed Reno mattresses in the Keurbooms Estuary. Zostera capensis extent was sampled by determining the percentage cover of 0.5 m X 0.5 m quadrats and measurements of eelgrass blades. Percentage cover and blade length decreased during the installation of Reno mattress, but then recovered shortly after completion of the installation. Abundances, richness and diversity of invertebrates and fish were found to be similar before and after the installation which suggests that the installation had no net negative impact on the site. The Reno mattresses were found to attract fauna typical of rocky shore environments as well as a few invasive alien invertebrate species. This study noted that a hybrid habitat of Reno mattress and eelgrass was created and may in fact provide the positives of both to a system. In any coastal development it will be important to balance the demands of a growing population and the protection of natural habitats. The results of this study suggest that complex artificial structures such as Reno mattresses do provide habitat for fish and invertebrates. However, the use of these structures should be in combination with natural vegetation (e.g. as a hybrid habitat) and not one that replaces intertidal and subtidal natural habitat especially eelgrass. There is limited information regarding the ecological impacts of using Reno mattresses in estuaries and this study provides new information on their ecological efficacy that should be valuable for future coastal erosion control practices.
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Economic evaluation of chemical and biological control methods on four aquatic weeds in South Africa

  • Authors: Maluleke, Mary
  • Date: 2020
  • Subjects: Invasive plants -- Biological control -- Economic aspects -- South Africa , Introduced organisms -- Biological control -- Economic aspects -- South Africa , Aquatic weeds -- Biological control -- Economic aspects -- South Africa , Aquatic weeds -- Control -- Economic aspects -- South Africa , Aquatic resources -- Management , Cost effectiveness , Net present value , Herbicides -- Cost effectiveness , Working for Water Programme , Water conservation -- South Africa
  • Language: English
  • Type: Thesis , Masters , MCom
  • Identifier: http://hdl.handle.net/10962/145953 , vital:38481
  • Description: Invasive alien plants (IAPs) of various kinds pose a threat to ecosystems, biodiversity, conservation and overall economy. In a world experiencing exponential increase in IAPs – this issue has become endemic, especially for developing countries such as South Africa. South Africa is a water scarce country and IAPs increase water stress. Thus, South Africa must invest in a more realistic, environmentally and economically inclusive policy outlook on the management of IAPs including aquatic weeds. This is especially urgent when considering the changing global climate, which is predicted to further reduce the quantity and quality of potable water. The Working for Water Programme (WfW) in South Africa aimed at addressing the issue of IAPs in a way that protects the environment as well as produces maximum return to society through poverty alleviation. As such, the aquatic weeds management strategy put in place for four of South Africa’s aquatic weeds Pista stratiotes, Salvinia molesta, Azolla filiculoides and Myriophyllum aquaticum - should be one that is cost-effective, efficient and sustainable; yielding the best possible return on investment. Since these four weeds are already under complete biological control, in the absence of biological agents, the WfW programme would have used herbicides to control these weeds. As such, this thesis conducted a retrospective analysis of the relative herbicide cost-saving associated with the use of biological control. To do this, due to existing limitations, E. crassipes was used as a surrogate weed and its herbicide control costs were used as proxy for the herbicide control cost estimates of the four selected weeds; with reasonable conversion factors applied to cater for the biological difference of the five weeds. Using the cost benefit analysis (CBA) framework, the net present cost (NPC) of each control method was calculated to which the relative cost-saving was considered to represent the avoided cost of using biological control instead of chemical control on these weeds. The avoided cost was used as the main benefit component when deriving the relative benefit cost ratios (BCR). Two scenarios were used, one assuming no follow-up requirement and the other assuming one follow-up requirement for chemical control. Using an 8% discount rate, the study found that the estimated cost of the biological control method on all four aquatic weeds was about R7,843,205 while for chemical control the estimated costs would have costed R149,580,142, R268,264,838 and R881,711,738 for application by means of a boat, bakkie and knapsack. Chemical control cost estimates would have increased to about R164,538,052, R295,216,120 and R1,008,761,000 for boat, bakkie and knapsack approach respectively when including a possible follow-up programme. These would have led to positive BCRs of 90.24:1, 164.97:1 and 557.99:1 across the three chemical control approaches without a follow-up (with BCR of about 99.67:1, 182.00:1 and 631.56:1 for the boat, bakkie and knapsack approach respectively with the accepted follow-up programme). When running a sensitivity test with varying discount rates of 5% and 10%, these results remained robust. As such, failing to reject the dominant hypothesis in literature, the main conclusion of the study is that biological control is indeed the more cost-effective management option compared to chemical control with respect to herbicide cost-saving. Further, biological control is most-likely to produce more environmental cost-saving and water-saving over chemical control. The study recommends the continued use of the biological control investment on the four aquatic weeds under study as well as on emerging aquatic weeds such as Iris pseudacorus, Nymphaea mexicana and Sagittaria platyphylla in South Africa.
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Effect of the nature of nanoparticles on the photophysicochemical properties and photodynamic antimicrobial chemotherapy of phthalocyanines

  • Authors: Magadla, Aviwe
  • Date: 2020
  • Subjects: Nanoparticles , Phthalocyanines , Anti-infective agents -- Therapeutic use , Photochemotherapy , Photochemistry
  • Language: English
  • Type: text , Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10962/123107 , vital:35406
  • Description: In this work, the syntheses and characterisation of Zn monocaffeic acid tri–tert–butyl phthalocyanine (1), Zn monocarboxyphenoxy tri– tert–butylphenoxyl phthalocyanine (2), tetrakis phenoxy N,N-dimethyl-4-(methylimino) phthalocyanine indium (III) chloride (3) and tetrakis N,N-dimethyl-4-(methylimino) phthalocyanine indium (III) chloride (5) are presented. Complexes 3 and 5 were further quartenised with 1,3- propanesultone to form corresponding complexes (4) and (6), respectively. Complexes 1 and 2 were covalently linked to amino functionalised nanoparticles (NPs). Complexes 3, 4, 5 and 6 where linked to oleic acid/oleylamine capped (OLA/OLM) silver-iron dimers (Ag-Fe3O4 OLA/OLM) and silver-iron core shell (Ag@Fe3O4 OLA/OLM) NPs via interaction between the nanoparticles and the imino group on the phthalocyanines. The phthalocyanine-NP conjugates afforded an increase in triplet quantum yields with a corresponding decrease in fluorescence quantum yield as compared to the phthalocyanine complexes alone. Complexes 3, 4 and their conjugates were then used for photodynamic antimicrobial chemotherapy on E. coli. The zwitterionic photosensitiser 4 and its conjugates showed better efficiency for photodynamic antimicrobial chemotherapy compared to their neutral counterparts.
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Effectiveness of different interventions to reduce occupational sitting among office administrators at Rhodes University

  • Authors: Malesa, Thato
  • Date: 2020
  • Subjects: Standing position , Sedentary behavior , Work environment , Employee health promotion , Office furniture -- Design , Industrial hygiene , Employees -- Health risk assessment , Human engineering , Compliance
  • Language: English
  • Type: text , Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10962/166403 , vital:41360
  • Description: Background: Sitting for prolonged periods is common in the working environment. Office workers are exposed to long periods of sitting time at work. Research has reported associations between prolonged sitting and negative health implications. As such studies have proposed different interventions aimed at reducing sitting times in the workplace. The aim of the current study was to evaluate the effectiveness of two different interventions, physical intervention (sit-stand worktables) and personal intervention (instruction to stretch) on compliance and reducing occupational sitting behaviour. The study also aimed to investigate the effectiveness of interventions in improving or impairing mood. Methods: The study took the form of a fieldbased study atRhodes University, Makhanda, South Africa. Fifteen full–time office workers in different divisions participated in the study. Participants were randomly allocated to either the physical or personal intervention group. In both intervention groups, partic ipants were prompted to stand for five minutes every hour during the workday to either work in a standing position or perform stretches. Over the course of the experiment, desk occupancy, sitting/standing time and mood effect were recorded in both intervention groups Both intervention groups were monitored for a period of three months (58 days). Results: The study found that the physical intervention group was an effective approach to reduce prolonged sitting in the workplace in comparison to the personal intervention group. Over the course of the experiment, there was sustain usage of interventions in both groups, however, in the physical intervention group desk usage decreased overtime. The findings of the study also show that some participants were more compliant with the study procedures than others. It was also found that mood improved upon introducing interventions in the workplace. However, with the outcomes of the results, the study acknowledges that several factors emerged which are likely to impact compliance, which future studies may investigate. Conclusion: Although sit-stand worktables are expensive, it seems like introducing them was successful in reducing sitting time and changing sitting behaviour in comparison to an intervention that instruct s individuals to stand up and stretch.
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Either way you die: a collection of short stories

Enabling violence: the ethics of writing and reading rape in South Africa

  • Authors: Lloyd Dylan Reumen
  • Date: 2020
  • Subjects: Rape -- South Africa , Rape -- Fiction , Rape in literature , Rape in literature -- South Africa , Psychic trauma in literature , Post-traumatic stress disorder in literature , Dystopias in literature , Coetzee, J. M., 1940- Disgrace , South African fiction (English) -- History and criticism
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: http://hdl.handle.net/10962/166173 , vital:41335
  • Description: This thesis is concerned with describing the stakes of reading, writing and criticising fictional depictions of rape in a country plagued by high levels of sexual violence. I consider the capacity of rape representations to cause harm to women and rape survivors, and worsen the various injuries suffered by survivors as a direct or indirect consequence of rape. The possibility of such harm prompts me to examine the role and responsibilities of readers and critics in facilitating or preventing such harm. I further discuss the potential strategies of harm prevention that readers of novelistic portrayals of rape might adopt as well as the positive outcomes that such reading strategies make possible, and which might balance out the risks that accompany them. My description of the potential harm of rape representations combines postmodern critical feminist analysis with Miranda Fricker’s work on epistemic justice and Judith Herman’s work on trauma in order to illustrate the way that these representations shape our conception of rape in a manner that affects everything from how it is enacted to our treatment of survivors to the possibility of their recovery from posttraumatic stress disorder. In order to situate my analysis in the context of South African literature and to explore the notion of responsibility in relation to the writing of scenes of rape, I utilise a close reading of J.M. Coetzee’s Disgrace. Furthermore, I discuss the utility and limits of the critical feminist strategy of using a normative critical approach to rape representations in order to prevent harm. Ultimately, I argue that the use of such a strategy, along with the development of a purpose-honed adaptive critical style, is essential to the fulfilment of our responsibilities as readers and to the prevention of further suffering.
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Enactment of hands-on practical activities through using easily accessible resources in a Grade 10 Physical Science classroom

  • Authors: Ndevahoma, Martha Kashike
  • Date: 2020
  • Subjects: Physical science -- Study and teaching (Secondary) -- Namibia
  • Language: English
  • Type: text , Thesis , Masters , MEd
  • Identifier: http://hdl.handle.net/10962/110229 , vital:33251
  • Description: The Namibian National Subject Policy Guide for Physical Science Grade 8-12 (MoE, 2010) indicates that science teachers should be creative and innovative in producing their own teaching and learning support materials that are linked to practice. Practical activities play a major role in the learning of science. Due to the lack of science laboratory equipment, however, minimal practical activities are done in some schools especially under-resourced disadvantaged schools. It is against this background that this case study investigated the possibilities and challenges associated with the use of easily accessible resources to carry out hands-on practical activities. Underpinned by an interpretive paradigm, this study used a mixed-method approach by generating both quantitative and qualitative data. It was carried out in the Otjozondjupa region in Namibia at Wato Secondary School (pseudonym). It was conducted with a Grade 10 Physical Science class consisting of 30 learners. To generate data, questionnaires, focus group interviews, lesson observations, stimulated recall interviews, as well as learners’ journal reflections were used. Conceptions, dispositions, interest and sense-making were used as conceptual lenses while Vygotsky’s social constructivism was the theoretical framework that informed this study. Quantitative data were analysed using the conceptual lenses and were subsequently presented in tables and graphs. That is, a deductive approach to analysis was used. On the other hand, a thematic approach was used to analyse the qualitative data. That is, qualitative data were inductively analysed to come up with sub-themes and themes. The findings from this study revealed significant shifts in learners’ conceptions, dispositions and interest towards science after the use of easily accessible resources to do hands-on practical activities. Similarly, learners’ sense-making of science concepts improved. This study thus recommends that science teachers should, where possible, make use of easily accessible resources to carry out hands-on practical activities during their science lessons. During such hands-on practicals, however, emphasis should be on the development of scientific concepts.
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