A short composition portfolio
- Buitendag, Kingsley Alexander
- Authors: Buitendag, Kingsley Alexander
- Date: 2011
- Subjects: Music -- South Africa Composition (Music)
- Language: English
- Type: text , Thesis , Masters , MMus
- Identifier: vital:2632 , http://hdl.handle.net/10962/d1002297
- Full Text:
- Authors: Buitendag, Kingsley Alexander
- Date: 2011
- Subjects: Music -- South Africa Composition (Music)
- Language: English
- Type: text , Thesis , Masters , MMus
- Identifier: vital:2632 , http://hdl.handle.net/10962/d1002297
- Full Text:
A strange counterpoint : classical music performance and identities in Grahamstown, South Africa
- Marais, Terence Wilmot Eugene
- Authors: Marais, Terence Wilmot Eugene
- Date: 2011
- Subjects: Music -- Western influences Music -- Performance -- Psychological aspects Music appreciation -- South Africa -- Grahamstown Music -- South Africa -- Grahamstown
- Language: English
- Type: Thesis , Masters , MMus
- Identifier: vital:2677 , http://hdl.handle.net/10962/d1007491
- Description: This study investigates the perceptions of South African practitioners of Western European Art Music (WEAM), specifically as they relate to the value of WEAM in contemporary South African society. In exploring some of the connections between musical identity and national identity, it sets out to discover what value WEAM holds for a certain group of student pianists. Qualitative empirical data was collected in the form of in-depth, semi-structured interviews and questionnaire responses, and the findings point to numerous, nuanced expressions of self and varied intersections of the nation with musical identity in the life of the individual. Further, WEAM appears to represent a crucial point of identification for these individuals, in each case generating positive affirmations of the self.
- Full Text:
- Authors: Marais, Terence Wilmot Eugene
- Date: 2011
- Subjects: Music -- Western influences Music -- Performance -- Psychological aspects Music appreciation -- South Africa -- Grahamstown Music -- South Africa -- Grahamstown
- Language: English
- Type: Thesis , Masters , MMus
- Identifier: vital:2677 , http://hdl.handle.net/10962/d1007491
- Description: This study investigates the perceptions of South African practitioners of Western European Art Music (WEAM), specifically as they relate to the value of WEAM in contemporary South African society. In exploring some of the connections between musical identity and national identity, it sets out to discover what value WEAM holds for a certain group of student pianists. Qualitative empirical data was collected in the form of in-depth, semi-structured interviews and questionnaire responses, and the findings point to numerous, nuanced expressions of self and varied intersections of the nation with musical identity in the life of the individual. Further, WEAM appears to represent a crucial point of identification for these individuals, in each case generating positive affirmations of the self.
- Full Text:
A study of a class of invariant optimal control problems on the Euclidean group SE(2)
- Authors: Adams, Ross Montague
- Date: 2011
- Subjects: Matrix groups Lie groups Extremal problems (Mathematics) Maximum principles (Mathematics) Hamilton-Jacobi equations Lyapunov stability
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5420 , http://hdl.handle.net/10962/d1006060
- Description: The aim of this thesis is to study a class of left-invariant optimal control problems on the matrix Lie group SE(2). We classify, under detached feedback equivalence, all controllable (left-invariant) control affine systems on SE(2). This result produces six types of control affine systems on SE(2). Hence, we study six associated left-invariant optimal control problems on SE(2). A left-invariant optimal control problem consists of minimizing a cost functional over the trajectory-control pairs of a left-invariant control system subject to appropriate boundary conditions. Each control problem is lifted from SE(2) to T*SE(2) ≅ SE(2) x se (2)*and then reduced to a problem on se (2)*. The maximum principle is used to obtain the optimal control and Hamiltonian corresponding to the normal extremals. Then we derive the (reduced) extremal equations on se (2)*. These equations are explicitly integrated by trigonometric and Jacobi elliptic functions. Finally, we fully classify, under Lyapunov stability, the equilibrium states of the normal extremal equations for each of the six types under consideration.
- Full Text:
- Authors: Adams, Ross Montague
- Date: 2011
- Subjects: Matrix groups Lie groups Extremal problems (Mathematics) Maximum principles (Mathematics) Hamilton-Jacobi equations Lyapunov stability
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5420 , http://hdl.handle.net/10962/d1006060
- Description: The aim of this thesis is to study a class of left-invariant optimal control problems on the matrix Lie group SE(2). We classify, under detached feedback equivalence, all controllable (left-invariant) control affine systems on SE(2). This result produces six types of control affine systems on SE(2). Hence, we study six associated left-invariant optimal control problems on SE(2). A left-invariant optimal control problem consists of minimizing a cost functional over the trajectory-control pairs of a left-invariant control system subject to appropriate boundary conditions. Each control problem is lifted from SE(2) to T*SE(2) ≅ SE(2) x se (2)*and then reduced to a problem on se (2)*. The maximum principle is used to obtain the optimal control and Hamiltonian corresponding to the normal extremals. Then we derive the (reduced) extremal equations on se (2)*. These equations are explicitly integrated by trigonometric and Jacobi elliptic functions. Finally, we fully classify, under Lyapunov stability, the equilibrium states of the normal extremal equations for each of the six types under consideration.
- Full Text:
Abortion as disruption: discourses surrounding abortion in the talk of men
- Authors: Hansjee, Jateen
- Date: 2011
- Subjects: Abortion -- Psychological aspects Abortion -- Social aspects Men -- South Africa -- Language Men -- South Africa -- Attitudes Health attitudes -- South Africa Discourse analysis -- Psychological aspects Discourse analysis -- Social aspects
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2984 , http://hdl.handle.net/10962/d1002493
- Description: This research examines men’s talk around abortion using critical discourse analysis. Current literature indicates a dearth of studies addressing the topic of men and abortion in various domains. An understanding of men’s relationship to abortion, however, is crucial to understanding abortion as a social phenomenon. This study utilises the work of Foucault around discourse and power, as well as Butler’s work on gender to create a theoretical framework to approach data. Data were collected in the form of interview groups made up of men, as well as newspaper articles and on-line forum discussions that featured men as the author. What emerged from theses texts was a ‘Familial Discourse’ which posits the nuclear, heterosexual family as a long term relationship between a mother and father, which forms the ideal site to raise children. Discourses that support the family are a discourse of ‘Equal Partnership’ which establishes the man and the woman as being in a heterosexual relationship where each partner is seen to have equal power, and a discourse of ‘Foetal Personhood’ which constructs the foetus as a child in need of a family. Related to the heterosexual matrix, the formation of a family unit comes to be constructed as ‘natural’. Abortion acts as a disruptor to these discourses. By disrupting the formation of the family unit, abortion negatively affects the individuals involved. A relationship where a formation of a family unit was disrupted cannot survive. If the female partner has an abortion without her partner, it is seen as disrupting the equal partnership between the man and the woman. Men in this case see themselves as ‘powerless’ compared to women. From this point a ‘New Man’ discourse emerges, where men position themselves as loving and responsible in the context of a nuclear, heterosexual family unit. Abortion disrupts ‘Foetal Personhood’ and is constructed as murder. In the case of rape the ‘Familial Discourse’ can be invoked either to justify abortion or resist abortion, based on whether or not a family unit can be formed. These discourses reproduce patriarchy.
- Full Text:
- Authors: Hansjee, Jateen
- Date: 2011
- Subjects: Abortion -- Psychological aspects Abortion -- Social aspects Men -- South Africa -- Language Men -- South Africa -- Attitudes Health attitudes -- South Africa Discourse analysis -- Psychological aspects Discourse analysis -- Social aspects
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2984 , http://hdl.handle.net/10962/d1002493
- Description: This research examines men’s talk around abortion using critical discourse analysis. Current literature indicates a dearth of studies addressing the topic of men and abortion in various domains. An understanding of men’s relationship to abortion, however, is crucial to understanding abortion as a social phenomenon. This study utilises the work of Foucault around discourse and power, as well as Butler’s work on gender to create a theoretical framework to approach data. Data were collected in the form of interview groups made up of men, as well as newspaper articles and on-line forum discussions that featured men as the author. What emerged from theses texts was a ‘Familial Discourse’ which posits the nuclear, heterosexual family as a long term relationship between a mother and father, which forms the ideal site to raise children. Discourses that support the family are a discourse of ‘Equal Partnership’ which establishes the man and the woman as being in a heterosexual relationship where each partner is seen to have equal power, and a discourse of ‘Foetal Personhood’ which constructs the foetus as a child in need of a family. Related to the heterosexual matrix, the formation of a family unit comes to be constructed as ‘natural’. Abortion acts as a disruptor to these discourses. By disrupting the formation of the family unit, abortion negatively affects the individuals involved. A relationship where a formation of a family unit was disrupted cannot survive. If the female partner has an abortion without her partner, it is seen as disrupting the equal partnership between the man and the woman. Men in this case see themselves as ‘powerless’ compared to women. From this point a ‘New Man’ discourse emerges, where men position themselves as loving and responsible in the context of a nuclear, heterosexual family unit. Abortion disrupts ‘Foetal Personhood’ and is constructed as murder. In the case of rape the ‘Familial Discourse’ can be invoked either to justify abortion or resist abortion, based on whether or not a family unit can be formed. These discourses reproduce patriarchy.
- Full Text:
Action competence and waste management: a case study of learner agency in two Grahamstown eco-schools
- Chiphwanya, Nellie Chimwemwe
- Authors: Chiphwanya, Nellie Chimwemwe
- Date: 2011
- Subjects: Environmental education -- South Africa -- Grahamstown -- Case studies Environmental education -- Study and teaching (Primary) -- South Africa -- Grahamstown -- Case studies Student-centered learning -- South Africa -- Grahamstown -- Case studies Refuse and refuse disposal -- Environmental aspects -- South Africa -- Grahamstown -- Case studies Recycling (Waste, etc) -- South Africa -- Grahamstown -- Case studies Active learning -- South Africa -- Grahamstown -- Case studies Competency based education -- South Africa -- Grahamstown -- Case studies
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1956 , http://hdl.handle.net/10962/d1008308
- Description: There has been a growing need in environmental education to develop students' ability and will to take part in democratic processes that enable them take environmental action in their local environment. This study examined learner action competence in waste management practices in two primary school contexts in the Eastern Cape, South Africa. An interpretive case study design is used to probe how learner participation in Eco-School waste management practices enabled the acquisition of knowledge in purposeful learning and action experiences that developed the vision and agency of informed action. The research was centred on two guiding questions: 1. How informed, purposeful and action-orientated is learner participation in Eco-School waste management activities? 2. What Eco-School waste management activities are fostering active participation towards a learner-led agency? Educators in the two schools were interviewed and Eco-School portfolios were examined for evidence of the learning activities and learner achievement. This provided the contextual data for reviewing focus group interviews to probe what was significant to learners, what they came to know and how they had contributed to the process of developing better waste management in the Eco-School context. The evidence generated in the study was used to identify the roles of the various players, the significant activities and processes that enabled and constrained the emergence of learner-led agency. The main findings in the study were that teacher intentionality and school management ethos were significant in engaging learners in meaningful waste management activities in both cases. The study also revealed that although most of the waste management activities in both cases were teacher-initiated, there were spaces open for learner initiatives. However, it appeared that the activity based waste management practices mostly allowed learners to learn how to do waste management more than allowing them to find out more about the scope and nature of ii the problem of waste. This then resulted in learners talking more about what they were doing with waste than talking about what they knew about waste. There were differences in the way in which learners approached waste in the two cases. In the one case, Kingswood Junior School learners used waste artistically and carried out activities that allowed for better use of waste resources like paper while in the other School, Grahamstown SDA School, learners approached waste as a resource for making money. However, in both cases, learners appeared to enjoy the positive experiences of doing things that contributed to a cleaner environment and were of benefit to others and this gave them a sense of pride to share their experiences with others. This study was significant as it allowed me to probe learner participation and examine the development of their action competence through listening to the voices of the learners themselves and understanding what was important to them about the knowledge they gained and their vision of better waste management.
- Full Text:
- Authors: Chiphwanya, Nellie Chimwemwe
- Date: 2011
- Subjects: Environmental education -- South Africa -- Grahamstown -- Case studies Environmental education -- Study and teaching (Primary) -- South Africa -- Grahamstown -- Case studies Student-centered learning -- South Africa -- Grahamstown -- Case studies Refuse and refuse disposal -- Environmental aspects -- South Africa -- Grahamstown -- Case studies Recycling (Waste, etc) -- South Africa -- Grahamstown -- Case studies Active learning -- South Africa -- Grahamstown -- Case studies Competency based education -- South Africa -- Grahamstown -- Case studies
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1956 , http://hdl.handle.net/10962/d1008308
- Description: There has been a growing need in environmental education to develop students' ability and will to take part in democratic processes that enable them take environmental action in their local environment. This study examined learner action competence in waste management practices in two primary school contexts in the Eastern Cape, South Africa. An interpretive case study design is used to probe how learner participation in Eco-School waste management practices enabled the acquisition of knowledge in purposeful learning and action experiences that developed the vision and agency of informed action. The research was centred on two guiding questions: 1. How informed, purposeful and action-orientated is learner participation in Eco-School waste management activities? 2. What Eco-School waste management activities are fostering active participation towards a learner-led agency? Educators in the two schools were interviewed and Eco-School portfolios were examined for evidence of the learning activities and learner achievement. This provided the contextual data for reviewing focus group interviews to probe what was significant to learners, what they came to know and how they had contributed to the process of developing better waste management in the Eco-School context. The evidence generated in the study was used to identify the roles of the various players, the significant activities and processes that enabled and constrained the emergence of learner-led agency. The main findings in the study were that teacher intentionality and school management ethos were significant in engaging learners in meaningful waste management activities in both cases. The study also revealed that although most of the waste management activities in both cases were teacher-initiated, there were spaces open for learner initiatives. However, it appeared that the activity based waste management practices mostly allowed learners to learn how to do waste management more than allowing them to find out more about the scope and nature of ii the problem of waste. This then resulted in learners talking more about what they were doing with waste than talking about what they knew about waste. There were differences in the way in which learners approached waste in the two cases. In the one case, Kingswood Junior School learners used waste artistically and carried out activities that allowed for better use of waste resources like paper while in the other School, Grahamstown SDA School, learners approached waste as a resource for making money. However, in both cases, learners appeared to enjoy the positive experiences of doing things that contributed to a cleaner environment and were of benefit to others and this gave them a sense of pride to share their experiences with others. This study was significant as it allowed me to probe learner participation and examine the development of their action competence through listening to the voices of the learners themselves and understanding what was important to them about the knowledge they gained and their vision of better waste management.
- Full Text:
Adjustment of commercial banks' interest rates and the effectiveness of monetary policy: evidence from Anglophone West Africa
- Authors: Bangura, Lamin
- Date: 2011
- Subjects: Monetary policy -- Africa, West , Banks and banking -- Africa, West , Interest rates -- Africa, West
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:951 , http://hdl.handle.net/10962/d1002685 , Monetary policy -- Africa, West , Banks and banking -- Africa, West , Interest rates -- Africa, West
- Description: Most central banks use short-term interest rates as their main instrument of monetary policy. It is assumed that a change in policy rate will influence interest rates set by commercial banks, but this is not usually the case. Commercial banks adjust their interest rates in response to changes in policy rate with lags, which make their interest rates sticky. Stickiness in commercial banks interest rates have been seen as an obstacle to the smooth transmission of monetary policy decisions. Despite the importance of the transmission process, little attention has been given to a systematic measurement of the degree of response of commercial banks‟ interest rates to changes in monetary policy stance in the Anglophone West African countries, specifically within the West African Monetary Zone (WAMZ) economies. Against this backdrop, this study explores the interest rate adjustment dynamics using monthly interest rate series on discount rate, treasury bill rate, commercial banks‟ deposit and lending rates from 1989 to 2009 (for Gambia, Nigeria and Sierra Leone) and from 2000 to 2009 (for Ghana). Specifically, the study set out to examine how lending and deposit rates respond to changes in the official rates and to see whether there is a convergence among the rates over time. Also, to examine the relative adjustment of commercial bank lending rates to changes in the official rate when there is disequilibrium. The analyses were twofold: a full sample period and a rolling window analysis. Following Cottarelli and Kourelis (1994), the study employed cointegration technique and an asymmetric error correction model to obtain the short-run and long-run parameters from which the error correction coefficients, mean adjustment lags and asymmetric mean adjustment lags were estimated. The results for the entire sample period revealed that the long-run pass-through in Nigeria was 81% and 67% for lending rates and deposit rates respectively. In Ghana, it was 66% and 69% for lending and deposit rates respectively. While in Sierra Leone, long-run pass-through was 62% and 72% for lending and deposit rates respectively. In Gambia, it was 50% and 40% for lending and deposit rates respectively. On the other hand, the short-run pass-through was found to be lower compared to the long-run pass-through: in Nigeria it was 66% and 47%; in Gambia, 26% and 29%; in Sierra Leone, 30% and 13%; and in Ghana, -6% and 35% for lending and deposit rates respectively in each country. The pass-through estimates for the rolling windows were mixed for short-run and long-run pass-through. The mean adjustment lags suggest that the speed of adjustment of Lending rates for full sample period were two, two, seven and twelve months in Nigeria, Ghana, Sierra Leone and Gambia respectively. While for deposit rates they were five, six, seven and eighteen for Ghana, Nigeria, Gambia and Sierra Leone respectively. The average speeds of adjustment for the rolling windows were four and five months for lending and deposit rates respectively. Weak evidence of convergence was found in lending and deposit rates in the short-run and long-run pass-through among the countries. However, the results suggest that the magnitude and speed of the pass-through amongst the countries on average were high compared to emerging Asian countries. Significant asymmetric adjustments were found in the lending rates for Gambia and Sierra Leone, while in Gambia and Nigeria there were asymmetries in deposit rates. Based on the evidence provided, interest rate pass-through is high in Nigeria and Ghana compared to Gambia and Sierra Leone and this calls for the harmonization of financial policies on the part of the financial authorities in the WAMZ. Viewed solely from an interest rate pass-through, the lack of convergence among the countries suggests that WAMZ is far from ready for a monetary union. The relatively low pass-through in some of the countries suggests rigidity in the banking system which may be due to underdevelopment of the system. Thus efforts geared toward strengthening the banking system and the financial system as whole would further enhance the prospect of a monetary union among them.
- Full Text:
- Authors: Bangura, Lamin
- Date: 2011
- Subjects: Monetary policy -- Africa, West , Banks and banking -- Africa, West , Interest rates -- Africa, West
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:951 , http://hdl.handle.net/10962/d1002685 , Monetary policy -- Africa, West , Banks and banking -- Africa, West , Interest rates -- Africa, West
- Description: Most central banks use short-term interest rates as their main instrument of monetary policy. It is assumed that a change in policy rate will influence interest rates set by commercial banks, but this is not usually the case. Commercial banks adjust their interest rates in response to changes in policy rate with lags, which make their interest rates sticky. Stickiness in commercial banks interest rates have been seen as an obstacle to the smooth transmission of monetary policy decisions. Despite the importance of the transmission process, little attention has been given to a systematic measurement of the degree of response of commercial banks‟ interest rates to changes in monetary policy stance in the Anglophone West African countries, specifically within the West African Monetary Zone (WAMZ) economies. Against this backdrop, this study explores the interest rate adjustment dynamics using monthly interest rate series on discount rate, treasury bill rate, commercial banks‟ deposit and lending rates from 1989 to 2009 (for Gambia, Nigeria and Sierra Leone) and from 2000 to 2009 (for Ghana). Specifically, the study set out to examine how lending and deposit rates respond to changes in the official rates and to see whether there is a convergence among the rates over time. Also, to examine the relative adjustment of commercial bank lending rates to changes in the official rate when there is disequilibrium. The analyses were twofold: a full sample period and a rolling window analysis. Following Cottarelli and Kourelis (1994), the study employed cointegration technique and an asymmetric error correction model to obtain the short-run and long-run parameters from which the error correction coefficients, mean adjustment lags and asymmetric mean adjustment lags were estimated. The results for the entire sample period revealed that the long-run pass-through in Nigeria was 81% and 67% for lending rates and deposit rates respectively. In Ghana, it was 66% and 69% for lending and deposit rates respectively. While in Sierra Leone, long-run pass-through was 62% and 72% for lending and deposit rates respectively. In Gambia, it was 50% and 40% for lending and deposit rates respectively. On the other hand, the short-run pass-through was found to be lower compared to the long-run pass-through: in Nigeria it was 66% and 47%; in Gambia, 26% and 29%; in Sierra Leone, 30% and 13%; and in Ghana, -6% and 35% for lending and deposit rates respectively in each country. The pass-through estimates for the rolling windows were mixed for short-run and long-run pass-through. The mean adjustment lags suggest that the speed of adjustment of Lending rates for full sample period were two, two, seven and twelve months in Nigeria, Ghana, Sierra Leone and Gambia respectively. While for deposit rates they were five, six, seven and eighteen for Ghana, Nigeria, Gambia and Sierra Leone respectively. The average speeds of adjustment for the rolling windows were four and five months for lending and deposit rates respectively. Weak evidence of convergence was found in lending and deposit rates in the short-run and long-run pass-through among the countries. However, the results suggest that the magnitude and speed of the pass-through amongst the countries on average were high compared to emerging Asian countries. Significant asymmetric adjustments were found in the lending rates for Gambia and Sierra Leone, while in Gambia and Nigeria there were asymmetries in deposit rates. Based on the evidence provided, interest rate pass-through is high in Nigeria and Ghana compared to Gambia and Sierra Leone and this calls for the harmonization of financial policies on the part of the financial authorities in the WAMZ. Viewed solely from an interest rate pass-through, the lack of convergence among the countries suggests that WAMZ is far from ready for a monetary union. The relatively low pass-through in some of the countries suggests rigidity in the banking system which may be due to underdevelopment of the system. Thus efforts geared toward strengthening the banking system and the financial system as whole would further enhance the prospect of a monetary union among them.
- Full Text:
African traditional medicine-antiretroviral interactions : effects of Sutherlandia frutescens on the pharmacokinetics of Atazanavir
- Authors: Müller, Adrienne Carmel
- Date: 2011 , 2011-03-28
- Subjects: Antiretroviral agents , Medicinal plants , Traditional medicine , AIDS (Disease) -- Treatment , HIV infections -- Drug therapy , Drug interactions , Pharmacokinetics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3859 , http://hdl.handle.net/10962/d1013373
- Description: In response to the urgent call for investigations into antiretroviral (ARV)-African traditional medicine (ATM) interactions, this research was undertaken to ascertain whether chronic administration of the ATM, Sutherlandia frutescens (SF) may alter the bioavailability of the protease inhibitor (PI), atazanavir (ATV), which may impact on the safety or efficacy of the ARV. Prior to investigating a potential interaction between ATV and SF in vitro and in vivo, a high performance liquid chromatography method with ultraviolet detection (HPLC-UV) was developed and validated for the bioanalysis of ATV in human plasma and liver microsomes. An improved and efficient analytical method with minimal use of solvents and short run time was achieved in comparison to methods published in the literature. In addition, the method was selective, linear, accurate and precise for quantitative analysis of ATV in these studies. Molecular docking studies were conducted to compare the binding modes and affinities of ATV and two major SF constituents, Sutherlandioside B and Sutherlandin C, with the efflux transporter, P-glycoprotein (P-gp) and the CYP450 isoenzyme, CYP3A4 to determine the potential for these phytochemicals to competitively inhibit the binding of ATV to these two proteins, which are mediators of absorption and metabolism. These studies revealed that modulation of P-gp transport of ATV by Sutherlandioside B and Sutherlandin C was not likely to occur via competitive inhibition. The results further indicated that weak competitive inhibition of CYP3A4 may possibly occur in the presence of either of these two SF constituents. The Caco-2 cell line was used as an in vitro model of human intestinal absorption. Accumulation studies in these cells were conducted to ascertain whether extracts and constituents of SF have the ability to alter the absorption of ATV. The results showed that the aqueous extract of SF significantly reduced ATV accumulation, suggesting decreased ATV absorption, whilst a triterpenoid glycoside fraction isolated from SF exhibited an opposing effect. Analogous responses were elicited by the aqueous extract and a triterpenoid glycoside fraction in similar accumulation studies in P-gp overexpressing Madin–Darby Canine Kidney Strain II cells (MDCKII-MDR1), which signified that the effects of this extract and component on ATV transport in the Caco-2 cells were P-gp-mediated. The quantitative analysis of ATV in human liver microsomes after co-incubation with extracts and components of SF was conducted to determine the effects of SF on the metabolism of ATV. The aqueous and methanolic extracts of SF inhibited ATV metabolism, whilst the triterpenoid glycoside fraction had a converse effect. Analogous effects by the extracts were demonstrated in experiments conducted in CYP3A4-transfected microsomes, suggesting that the inhibition of ATV metabolism in the liver microsomes by these SF extracts was CYP3A4-mediated. A combination of Sutherlandiosides C and D also inhibited CYP3A4-mediated ATV metabolism, which was in contrast to the response elicited by the triterpenoid fraction in the liver microsomes, where other unidentified compounds, shown to be present therein, may have contributed to the activation of ATV metabolism. The in vitro studies revealed the potential for SF to alter the bioavailability of ATV, therefore a clinical study in which the effect of a multiple dose regimen of SF on the pharmacokinetics (PK) of a single dose of ATV was conducted in healthy male volunteers. The statistical analysis showed that the 90 % confidence intervals around the geometric mean ratios (ATV + SF/ATV alone) for both Cmax and AUC0-24 hours, fell well below the lower limit of the "no-effect" boundary of 0.8 – 1.25, implying that the bioavailability of ATV was significantly reduced in this cohort of subjects. It may thus be concluded that if the reduction in bioavailability observed in this clinical study is found to be clinically relevant, co-administration of SF commercial dosage forms and ATV in HIV/AIDS patients may potentially result in subtherapeutic ATV levels, which may in turn contribute to ATV resistance and/or treatment failure. This research has therefore highlighted the potential risk for toxicity or lack of efficacy of ARV regimens which may result when ATMs and PIs are used concurrently and that patients and health care practitioners alike should be aware of these perils.
- Full Text:
- Authors: Müller, Adrienne Carmel
- Date: 2011 , 2011-03-28
- Subjects: Antiretroviral agents , Medicinal plants , Traditional medicine , AIDS (Disease) -- Treatment , HIV infections -- Drug therapy , Drug interactions , Pharmacokinetics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3859 , http://hdl.handle.net/10962/d1013373
- Description: In response to the urgent call for investigations into antiretroviral (ARV)-African traditional medicine (ATM) interactions, this research was undertaken to ascertain whether chronic administration of the ATM, Sutherlandia frutescens (SF) may alter the bioavailability of the protease inhibitor (PI), atazanavir (ATV), which may impact on the safety or efficacy of the ARV. Prior to investigating a potential interaction between ATV and SF in vitro and in vivo, a high performance liquid chromatography method with ultraviolet detection (HPLC-UV) was developed and validated for the bioanalysis of ATV in human plasma and liver microsomes. An improved and efficient analytical method with minimal use of solvents and short run time was achieved in comparison to methods published in the literature. In addition, the method was selective, linear, accurate and precise for quantitative analysis of ATV in these studies. Molecular docking studies were conducted to compare the binding modes and affinities of ATV and two major SF constituents, Sutherlandioside B and Sutherlandin C, with the efflux transporter, P-glycoprotein (P-gp) and the CYP450 isoenzyme, CYP3A4 to determine the potential for these phytochemicals to competitively inhibit the binding of ATV to these two proteins, which are mediators of absorption and metabolism. These studies revealed that modulation of P-gp transport of ATV by Sutherlandioside B and Sutherlandin C was not likely to occur via competitive inhibition. The results further indicated that weak competitive inhibition of CYP3A4 may possibly occur in the presence of either of these two SF constituents. The Caco-2 cell line was used as an in vitro model of human intestinal absorption. Accumulation studies in these cells were conducted to ascertain whether extracts and constituents of SF have the ability to alter the absorption of ATV. The results showed that the aqueous extract of SF significantly reduced ATV accumulation, suggesting decreased ATV absorption, whilst a triterpenoid glycoside fraction isolated from SF exhibited an opposing effect. Analogous responses were elicited by the aqueous extract and a triterpenoid glycoside fraction in similar accumulation studies in P-gp overexpressing Madin–Darby Canine Kidney Strain II cells (MDCKII-MDR1), which signified that the effects of this extract and component on ATV transport in the Caco-2 cells were P-gp-mediated. The quantitative analysis of ATV in human liver microsomes after co-incubation with extracts and components of SF was conducted to determine the effects of SF on the metabolism of ATV. The aqueous and methanolic extracts of SF inhibited ATV metabolism, whilst the triterpenoid glycoside fraction had a converse effect. Analogous effects by the extracts were demonstrated in experiments conducted in CYP3A4-transfected microsomes, suggesting that the inhibition of ATV metabolism in the liver microsomes by these SF extracts was CYP3A4-mediated. A combination of Sutherlandiosides C and D also inhibited CYP3A4-mediated ATV metabolism, which was in contrast to the response elicited by the triterpenoid fraction in the liver microsomes, where other unidentified compounds, shown to be present therein, may have contributed to the activation of ATV metabolism. The in vitro studies revealed the potential for SF to alter the bioavailability of ATV, therefore a clinical study in which the effect of a multiple dose regimen of SF on the pharmacokinetics (PK) of a single dose of ATV was conducted in healthy male volunteers. The statistical analysis showed that the 90 % confidence intervals around the geometric mean ratios (ATV + SF/ATV alone) for both Cmax and AUC0-24 hours, fell well below the lower limit of the "no-effect" boundary of 0.8 – 1.25, implying that the bioavailability of ATV was significantly reduced in this cohort of subjects. It may thus be concluded that if the reduction in bioavailability observed in this clinical study is found to be clinically relevant, co-administration of SF commercial dosage forms and ATV in HIV/AIDS patients may potentially result in subtherapeutic ATV levels, which may in turn contribute to ATV resistance and/or treatment failure. This research has therefore highlighted the potential risk for toxicity or lack of efficacy of ARV regimens which may result when ATMs and PIs are used concurrently and that patients and health care practitioners alike should be aware of these perils.
- Full Text:
After Baines
- Walters, John Attwood Vereker
- Authors: Walters, John Attwood Vereker
- Date: 2011
- Subjects: Baines, Thomas, 1820-1875 Baines, Thomas, 1820-1875 -- Biography Painting, South African -- Exhibitions Painters -- South Africa -- Biography
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2432 , http://hdl.handle.net/10962/d1004368
- Description: By researching the life and work of Thomas Baines (1820 - 1875) in relation to a broader discourse of painting and the lived experience of being a 'white' male in a post-apartheid South Africa, I explore the ways in which this figure from the past has provoked the three series of artworks I have produced for my Master of Fine Art exhibition. This study has been divided into two parts, represented by the two chapters contained herein. Chapter One includes a critical retelling of Baines' biography and a discussion of the primary ways in which I have engaged with both the life and the working practice of this artist. I also address my own personal complicity in the constructions of 'the figure of Baines' as I have framed him both visually and textually during my work for this degree. Chapter Two describes some of the practicalities of my working process as a visual artist, including how I understand the theoretical and conceptual concerns which I raise in Chapter One to be visually manifest in my work. In this chapter, I also discuss my work in relation to the work of the contemporary South African artists William Kentridge and Johannes Phokela. The artistic practice of one artist imitating another artist's work is also explored as a central conceptual thread which could be seen to weave my verbal and visual production together.
- Full Text:
- Authors: Walters, John Attwood Vereker
- Date: 2011
- Subjects: Baines, Thomas, 1820-1875 Baines, Thomas, 1820-1875 -- Biography Painting, South African -- Exhibitions Painters -- South Africa -- Biography
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2432 , http://hdl.handle.net/10962/d1004368
- Description: By researching the life and work of Thomas Baines (1820 - 1875) in relation to a broader discourse of painting and the lived experience of being a 'white' male in a post-apartheid South Africa, I explore the ways in which this figure from the past has provoked the three series of artworks I have produced for my Master of Fine Art exhibition. This study has been divided into two parts, represented by the two chapters contained herein. Chapter One includes a critical retelling of Baines' biography and a discussion of the primary ways in which I have engaged with both the life and the working practice of this artist. I also address my own personal complicity in the constructions of 'the figure of Baines' as I have framed him both visually and textually during my work for this degree. Chapter Two describes some of the practicalities of my working process as a visual artist, including how I understand the theoretical and conceptual concerns which I raise in Chapter One to be visually manifest in my work. In this chapter, I also discuss my work in relation to the work of the contemporary South African artists William Kentridge and Johannes Phokela. The artistic practice of one artist imitating another artist's work is also explored as a central conceptual thread which could be seen to weave my verbal and visual production together.
- Full Text:
An analysis of sources and predictability of geomagnetic storms
- Authors: Uwamahoro, Jean
- Date: 2011
- Subjects: Ionospheric storms Solar flares Interplanetary magnetic fields Magnetospheric substorms Coronal mass ejections Space environment Neural networks (Computer science)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5451 , http://hdl.handle.net/10962/d1005236
- Description: Solar transient eruptions are the main cause of interplanetary-magnetospheric disturbances leading to the phenomena known as geomagnetic storms. Eruptive solar events such as coronal mass ejections (CMEs) are currently considered the main cause of geomagnetic storms (GMS). GMS are strong perturbations of the Earth’s magnetic field that can affect space-borne and ground-based technological systems. The solar-terrestrial impact on modern technological systems is commonly known as Space Weather. Part of the research study described in this thesis was to investigate and establish a relationship between GMS (periods with Dst ≤ −50 nT) and their associated solar and interplanetary (IP) properties during solar cycle (SC) 23. Solar and IP geoeffective properties associated with or without CMEs were investigated and used to qualitatively characterise both intense and moderate storms. The results of this analysis specifically provide an estimate of the main sources of GMS during an average 11-year solar activity period. This study indicates that during SC 23, the majority of intense GMS (83%) were associated with CMEs, while the non-associated CME storms were dominant among moderate storms. GMS phenomena are the result of a complex and non-linear chaotic system involving the Sun, the IP medium, the magnetosphere and ionosphere, which make the prediction of these phenomena challenging. This thesis also explored the predictability of both the occurrence and strength of GMS. Due to their nonlinear driving mechanisms, the prediction of GMS was attempted by the use of neural network (NN) techniques, known for their non-linear modelling capabilities. To predict the occurrence of storms, a combination of solar and IP parameters were used as inputs in the NN model that proved to predict the occurrence of GMS with a probability of 87%. Using the solar wind (SW) and IP magnetic field (IMF) parameters, a separate NN-based model was developed to predict the storm-time strength as measured by the global Dst and ap geomagnetic indices, as well as by the locally measured K-index. The performance of the models was tested on data sets which were not part of the NN training process. The results obtained indicate that NN models provide a reliable alternative method for empirically predicting the occurrence and strength of GMS on the basis of solar and IP parameters. The demonstrated ability to predict the geoeffectiveness of solar and IP transient events is a key step in the goal towards improving space weather modelling and prediction.
- Full Text:
- Authors: Uwamahoro, Jean
- Date: 2011
- Subjects: Ionospheric storms Solar flares Interplanetary magnetic fields Magnetospheric substorms Coronal mass ejections Space environment Neural networks (Computer science)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5451 , http://hdl.handle.net/10962/d1005236
- Description: Solar transient eruptions are the main cause of interplanetary-magnetospheric disturbances leading to the phenomena known as geomagnetic storms. Eruptive solar events such as coronal mass ejections (CMEs) are currently considered the main cause of geomagnetic storms (GMS). GMS are strong perturbations of the Earth’s magnetic field that can affect space-borne and ground-based technological systems. The solar-terrestrial impact on modern technological systems is commonly known as Space Weather. Part of the research study described in this thesis was to investigate and establish a relationship between GMS (periods with Dst ≤ −50 nT) and their associated solar and interplanetary (IP) properties during solar cycle (SC) 23. Solar and IP geoeffective properties associated with or without CMEs were investigated and used to qualitatively characterise both intense and moderate storms. The results of this analysis specifically provide an estimate of the main sources of GMS during an average 11-year solar activity period. This study indicates that during SC 23, the majority of intense GMS (83%) were associated with CMEs, while the non-associated CME storms were dominant among moderate storms. GMS phenomena are the result of a complex and non-linear chaotic system involving the Sun, the IP medium, the magnetosphere and ionosphere, which make the prediction of these phenomena challenging. This thesis also explored the predictability of both the occurrence and strength of GMS. Due to their nonlinear driving mechanisms, the prediction of GMS was attempted by the use of neural network (NN) techniques, known for their non-linear modelling capabilities. To predict the occurrence of storms, a combination of solar and IP parameters were used as inputs in the NN model that proved to predict the occurrence of GMS with a probability of 87%. Using the solar wind (SW) and IP magnetic field (IMF) parameters, a separate NN-based model was developed to predict the storm-time strength as measured by the global Dst and ap geomagnetic indices, as well as by the locally measured K-index. The performance of the models was tested on data sets which were not part of the NN training process. The results obtained indicate that NN models provide a reliable alternative method for empirically predicting the occurrence and strength of GMS on the basis of solar and IP parameters. The demonstrated ability to predict the geoeffectiveness of solar and IP transient events is a key step in the goal towards improving space weather modelling and prediction.
- Full Text:
An analysis of the influence of domestic macroeconomic variables on the performance of the South African stock market sectoral indices
- Authors: Hancocks, Ryan Lee
- Date: 2011
- Subjects: Johannesburg Stock Exchange , Stock price indexes -- South Africa , Interest rates -- South Africa , Macroeconomics -- Econometric models
- Language: English
- Type: text , Thesis , Masters , MCom
- Identifier: vital:954
- Description: An econometric study was undertaken to determine the extent to which selected macroeconomic variables influenced stock market prices on the All-Share, Financial, Mining and Retail Indices of the Johannesburg Stock Exchange in South Africa from 1996:7 to 2008:12. The Johansen cointegration method was used to determine whether cointegrating relationships existed between the macroeconomic variables and the stock market indices. A Vector Error Correction model was estimated and found significant results that money supply, inflation, long and short- run interest rates, and the exchange rate all had an influence on stock market prices. Further, the VECM results for each sector indicated that certain macroeconomic variables had differing influences on each sector of the stock market. Impulse Response tests indicated that the selected macroeconomic variables caused shock to the sectoral indices in the short-run but that the effect was lagged. The Variance Decomposition tests conducted supported earlier evidence that the macroeconomic variables had a strong level of sectoral index determinacy in the long run. The results found that the All-Share and Financial Indices were negatively influenced by inflation and short term interest rates in the long run, while the Financial Index also had a negative relationship with long-run interest rates. Money supply was found to have a positive effect on all the indices. A weakening of the exchange rate was found to have a positive influence on both the Retail and Mining Indices, while it negatively affected the All-Share and Financial Indices. The long-run interest rate had a positive influence on both the Retail and Mining Indices. Overall the study finds that macroeconomic variables are important determinants of stock market prices in South Africa and that it is important to examine each sector of the stock market separately to capture what are different effects. The limitations of the study are that a different measure for exchange rates from the nominal rand/dollar exchange rate used here may yield more decisive results and provide insight into the link between exchange rate behaviour and performance of especially the retail sector.
- Full Text:
- Authors: Hancocks, Ryan Lee
- Date: 2011
- Subjects: Johannesburg Stock Exchange , Stock price indexes -- South Africa , Interest rates -- South Africa , Macroeconomics -- Econometric models
- Language: English
- Type: text , Thesis , Masters , MCom
- Identifier: vital:954
- Description: An econometric study was undertaken to determine the extent to which selected macroeconomic variables influenced stock market prices on the All-Share, Financial, Mining and Retail Indices of the Johannesburg Stock Exchange in South Africa from 1996:7 to 2008:12. The Johansen cointegration method was used to determine whether cointegrating relationships existed between the macroeconomic variables and the stock market indices. A Vector Error Correction model was estimated and found significant results that money supply, inflation, long and short- run interest rates, and the exchange rate all had an influence on stock market prices. Further, the VECM results for each sector indicated that certain macroeconomic variables had differing influences on each sector of the stock market. Impulse Response tests indicated that the selected macroeconomic variables caused shock to the sectoral indices in the short-run but that the effect was lagged. The Variance Decomposition tests conducted supported earlier evidence that the macroeconomic variables had a strong level of sectoral index determinacy in the long run. The results found that the All-Share and Financial Indices were negatively influenced by inflation and short term interest rates in the long run, while the Financial Index also had a negative relationship with long-run interest rates. Money supply was found to have a positive effect on all the indices. A weakening of the exchange rate was found to have a positive influence on both the Retail and Mining Indices, while it negatively affected the All-Share and Financial Indices. The long-run interest rate had a positive influence on both the Retail and Mining Indices. Overall the study finds that macroeconomic variables are important determinants of stock market prices in South Africa and that it is important to examine each sector of the stock market separately to capture what are different effects. The limitations of the study are that a different measure for exchange rates from the nominal rand/dollar exchange rate used here may yield more decisive results and provide insight into the link between exchange rate behaviour and performance of especially the retail sector.
- Full Text:
An analysis of the long run comovements between financial system development and mining production in South Africa
- Authors: Ajagbe, Stephen Mayowa
- Date: 2011
- Subjects: Economic development -- South Africa , Econometric models , Mineral industries -- Economic aspects -- South Africa , South Africa -- Economic conditions , South Africa -- Economic policy , Principal components analysis , Cointegration , Stock exchanges -- South Africa , Banks and banking -- South Africa , Foreign exchange rates
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:955 , http://hdl.handle.net/10962/d1002689 , Economic development -- South Africa , Econometric models , Mineral industries -- Economic aspects -- South Africa , South Africa -- Economic conditions , South Africa -- Economic policy , Principal components analysis , Cointegration , Stock exchanges -- South Africa , Banks and banking -- South Africa , Foreign exchange rates
- Description: This study examines the nature of the relationship which exists between mining sector production and development of the financial systems in South Africa. This is particularly important in that the mining sector is considered to be one of the major contributors to the country’s overall economic growth. South Africa is also considered to have a very well developed financial system, to the point where the dominance of one over the other is difficult to identify. Therefore offering insight into the nature of this relationship will assist policy makers in identifying the most effective policies in order to ensure that the developments within the financial systems impact appropriately on the mining sector, and ultimately on the economy. In addition to using the conventional proxies of financial system development, this study utilises the principal component analysis (PCA) to construct an index for the entire financial system. The multivariate cointegration approach as proposed by Johansen (1988) and Johansen and Juselius (1990) was then used to estimate the relationship between the development of the financial systems and the mining sector production for the period 1988-2008. The study reveals mixed results for different measures of financial system development. Those involving the banking system show that a negative relationship exists between total mining production and total credit extended to the private sector, while liquid liabilities has a positive relationship. Similarly, with the stock market system, mixed results are also obtained which reveal a negative relationship between total mining production and stock market capitalisation, while a positive relationship is found with secondary market turnover. Of all the financial system variables, only that of stock market capitalisation was found to be significant. The result with the financial development index reveals that a significant negative relationship exists between financial system development and total mining sector production. Results on the other variables controlled in the estimation show that positive and significant relationships exist between total mining production and both nominal exchange rate and political stability respectively. Increased mining production therefore takes place in periods of appreciating exchange rates, and similarly in the post-apartheid era. On the other hand, negative relationships were found for both trade openness and inflation control variables. The impulse response and variance decomposition analyses showed that total mining production explains the largest amount of shocks within itself. Overall, the study reveals that the mining sector might not have benefited much from the development in the South African financial system.
- Full Text:
- Authors: Ajagbe, Stephen Mayowa
- Date: 2011
- Subjects: Economic development -- South Africa , Econometric models , Mineral industries -- Economic aspects -- South Africa , South Africa -- Economic conditions , South Africa -- Economic policy , Principal components analysis , Cointegration , Stock exchanges -- South Africa , Banks and banking -- South Africa , Foreign exchange rates
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:955 , http://hdl.handle.net/10962/d1002689 , Economic development -- South Africa , Econometric models , Mineral industries -- Economic aspects -- South Africa , South Africa -- Economic conditions , South Africa -- Economic policy , Principal components analysis , Cointegration , Stock exchanges -- South Africa , Banks and banking -- South Africa , Foreign exchange rates
- Description: This study examines the nature of the relationship which exists between mining sector production and development of the financial systems in South Africa. This is particularly important in that the mining sector is considered to be one of the major contributors to the country’s overall economic growth. South Africa is also considered to have a very well developed financial system, to the point where the dominance of one over the other is difficult to identify. Therefore offering insight into the nature of this relationship will assist policy makers in identifying the most effective policies in order to ensure that the developments within the financial systems impact appropriately on the mining sector, and ultimately on the economy. In addition to using the conventional proxies of financial system development, this study utilises the principal component analysis (PCA) to construct an index for the entire financial system. The multivariate cointegration approach as proposed by Johansen (1988) and Johansen and Juselius (1990) was then used to estimate the relationship between the development of the financial systems and the mining sector production for the period 1988-2008. The study reveals mixed results for different measures of financial system development. Those involving the banking system show that a negative relationship exists between total mining production and total credit extended to the private sector, while liquid liabilities has a positive relationship. Similarly, with the stock market system, mixed results are also obtained which reveal a negative relationship between total mining production and stock market capitalisation, while a positive relationship is found with secondary market turnover. Of all the financial system variables, only that of stock market capitalisation was found to be significant. The result with the financial development index reveals that a significant negative relationship exists between financial system development and total mining sector production. Results on the other variables controlled in the estimation show that positive and significant relationships exist between total mining production and both nominal exchange rate and political stability respectively. Increased mining production therefore takes place in periods of appreciating exchange rates, and similarly in the post-apartheid era. On the other hand, negative relationships were found for both trade openness and inflation control variables. The impulse response and variance decomposition analyses showed that total mining production explains the largest amount of shocks within itself. Overall, the study reveals that the mining sector might not have benefited much from the development in the South African financial system.
- Full Text:
An art based support programme for the amelioration of general psychological distress in marginalised children in South Africa
- Authors: Armstrong, Meredith
- Date: 2011 , 2011-06-28
- Subjects: Art therapy for children Child mental health Group psychotherapy for children Abused children -- Mental health
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3096 , http://hdl.handle.net/10962/d1003730
- Description: This study aimed to construct, and implement an in-school "art expression" based programme designed to aid in mitigation of psychological stress, behavioural difficulties, and self-negativity frequently experienced by marginalised children. It was developed for application within schools that cater for children exposed to neglect and/or abuse resulting from poverty in South Africa. This programme outline was developed and implemented through intensive exploration and review of previously effective art therapy, art expressive methods and techniques in similar contexts, together with the knowledge and experience of a qualified art therapist and educational psychologist. Data was collected through open-ended informal qualitative interviews, observations, and photographs of artworks produced during sessions. These were then analysed in conjunction, using the content analysis method, visual interpretive measures and thematic analysis. This enquiry documented the process of art creation through "art expression", and its ability to ameliorate psychological difficulties affecting marginalised children in South Africa. Following the establishment of trust between the researchers and the participants, the results found that the use of different art modalities demonstrated predominantly positive results in varying degrees. It is hoped that this study can be used to further practical interventions of this nature in comparable milieus in South Africa.
- Full Text:
- Authors: Armstrong, Meredith
- Date: 2011 , 2011-06-28
- Subjects: Art therapy for children Child mental health Group psychotherapy for children Abused children -- Mental health
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3096 , http://hdl.handle.net/10962/d1003730
- Description: This study aimed to construct, and implement an in-school "art expression" based programme designed to aid in mitigation of psychological stress, behavioural difficulties, and self-negativity frequently experienced by marginalised children. It was developed for application within schools that cater for children exposed to neglect and/or abuse resulting from poverty in South Africa. This programme outline was developed and implemented through intensive exploration and review of previously effective art therapy, art expressive methods and techniques in similar contexts, together with the knowledge and experience of a qualified art therapist and educational psychologist. Data was collected through open-ended informal qualitative interviews, observations, and photographs of artworks produced during sessions. These were then analysed in conjunction, using the content analysis method, visual interpretive measures and thematic analysis. This enquiry documented the process of art creation through "art expression", and its ability to ameliorate psychological difficulties affecting marginalised children in South Africa. Following the establishment of trust between the researchers and the participants, the results found that the use of different art modalities demonstrated predominantly positive results in varying degrees. It is hoped that this study can be used to further practical interventions of this nature in comparable milieus in South Africa.
- Full Text:
An evaluation of the feasibility of obtaining payment for ecosystem services for the Baviaanskloof Nature Reserve
- Authors: Erlank, Wayne Michael
- Date: 2011
- Subjects: Ecosystem services -- South Africa -- Eastern Cape Ecology -- Economic aspects -- South Africa -- Eastern Cape Environmental economics -- South Africa -- Eastern Cape Water-supply -- South Africa -- Eastern Cape Water-supply -- South Africa -- Eastern Cape -- Management Water resources development -- South Africa -- Eastern Cape Nature conservation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:742 , http://hdl.handle.net/10962/d1003862
- Description: Cities must go further and further away to find new, more costly sources of water for human consumption while industries and agriculture continue to compete for increasingly scarce water resources. This may already be seen occurring within the Nelson Mandela Bay Metro where the severe drought being experienced during the past 18 months has severely depleted water supply dams. One of the main supply dams to the Nelson Mandela Bay Municipality is situated within the Baviaanskloof Nature Reserve and World Heritage Site. The potential of funding the Baviaanskloof Nature Reserve and World Heritage Site with payments for ecosystem services (water) obtained for water services supplied to the Nelson Mandela Bay Municipalities and agriculture in the Gamtoos River Valley will ensure financial sustainable for the Baviaanskloof Nature Reserve and World Heritage Site in the long term. This ability to become financially independent and generate its own income will place the Baviaanskloof Nature Reserve and World Heritage Site in a unique position within the conservation community in South Africa as only a very few protected areas are self sustaining through payment for an ecosystem service.
- Full Text:
- Authors: Erlank, Wayne Michael
- Date: 2011
- Subjects: Ecosystem services -- South Africa -- Eastern Cape Ecology -- Economic aspects -- South Africa -- Eastern Cape Environmental economics -- South Africa -- Eastern Cape Water-supply -- South Africa -- Eastern Cape Water-supply -- South Africa -- Eastern Cape -- Management Water resources development -- South Africa -- Eastern Cape Nature conservation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:742 , http://hdl.handle.net/10962/d1003862
- Description: Cities must go further and further away to find new, more costly sources of water for human consumption while industries and agriculture continue to compete for increasingly scarce water resources. This may already be seen occurring within the Nelson Mandela Bay Metro where the severe drought being experienced during the past 18 months has severely depleted water supply dams. One of the main supply dams to the Nelson Mandela Bay Municipality is situated within the Baviaanskloof Nature Reserve and World Heritage Site. The potential of funding the Baviaanskloof Nature Reserve and World Heritage Site with payments for ecosystem services (water) obtained for water services supplied to the Nelson Mandela Bay Municipalities and agriculture in the Gamtoos River Valley will ensure financial sustainable for the Baviaanskloof Nature Reserve and World Heritage Site in the long term. This ability to become financially independent and generate its own income will place the Baviaanskloof Nature Reserve and World Heritage Site in a unique position within the conservation community in South Africa as only a very few protected areas are self sustaining through payment for an ecosystem service.
- Full Text:
An evaluation of the marketing and distribution channels of the Mzuzu Coffee Planters Cooperative Union (MZCPCU)
- Jussa, Leman Abdulrazak Wyson
- Authors: Jussa, Leman Abdulrazak Wyson
- Date: 2011
- Subjects: Mzuzu Coffee Planters Cooperative Union Coffee -- Marketing -- Malawi Marketing channels -- Evaluation -- Malawi Agricultural development projects -- Malawi
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:819 , http://hdl.handle.net/10962/d1008300
- Description: Literature and the cooperative movement in Malawi shows that the first cooperative was established in 1947. Since the economy of the country depends on agriculture, most cooperatives were strongly developed in the fields of agricultural production, and processing and marketing. The first coffee cooperative was registered in 1950, known as the Nchenachena Coffee Producers Cooperative Society. After Malawi 's independence in 1964, agricultural cooperatives were dissolved and government formed the Farmers Marketing Board (FMB) to take over all marketing activities of all crops, including coffee. The FMB was replaced by the Agricultural Development and Marketing Corporation (ADMARC) in 1967. After the dissolution of agricultural cooperatives, government saw the need for crop development and established Smallholder Coffee Authority in 1971. The Smallholder Coffee Authority worked up to 1999 when it collapsed because of administrative and financial problems. Due to the failure of the Smallholder Coffee Authority, the government of Malawi reintroduced agricultural cooperatives in 1999. Mzuzu Coffee Planters ' Cooperative Union was formed in 2006 following the re-introduction of cooperatives. The union is mandated to promote sustainable production, processing and marketing of Arabica coffee on behalf of the farmers. Since its inception, the output of coffee for export from the Mzuzu Coffee Planters Cooperative Union has increased from 156 tonnes in 2006 to over 400 tonnes in 2009. The increase in coffee exports was not by mere chance but a number of factors contributed to this success. The main reasons for this success were: • Strong prior relationship between the growers and the buyers • The uniqueness of the cooperative business model • The unique taste and quality of coffee grown • The introduction of a different approach to farming coffee Currently there is high demand for organic coffee and this could be a niche market that is not only rewarded with premium prices but also creates environmental friendly business. The union considers Fair Trade market as an alternative approach to conventional trade, aiming at improving the livelihoods of smallholder producers by improving payments, giving them fair prices and providing continuity in trading relationships.
- Full Text:
- Authors: Jussa, Leman Abdulrazak Wyson
- Date: 2011
- Subjects: Mzuzu Coffee Planters Cooperative Union Coffee -- Marketing -- Malawi Marketing channels -- Evaluation -- Malawi Agricultural development projects -- Malawi
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:819 , http://hdl.handle.net/10962/d1008300
- Description: Literature and the cooperative movement in Malawi shows that the first cooperative was established in 1947. Since the economy of the country depends on agriculture, most cooperatives were strongly developed in the fields of agricultural production, and processing and marketing. The first coffee cooperative was registered in 1950, known as the Nchenachena Coffee Producers Cooperative Society. After Malawi 's independence in 1964, agricultural cooperatives were dissolved and government formed the Farmers Marketing Board (FMB) to take over all marketing activities of all crops, including coffee. The FMB was replaced by the Agricultural Development and Marketing Corporation (ADMARC) in 1967. After the dissolution of agricultural cooperatives, government saw the need for crop development and established Smallholder Coffee Authority in 1971. The Smallholder Coffee Authority worked up to 1999 when it collapsed because of administrative and financial problems. Due to the failure of the Smallholder Coffee Authority, the government of Malawi reintroduced agricultural cooperatives in 1999. Mzuzu Coffee Planters ' Cooperative Union was formed in 2006 following the re-introduction of cooperatives. The union is mandated to promote sustainable production, processing and marketing of Arabica coffee on behalf of the farmers. Since its inception, the output of coffee for export from the Mzuzu Coffee Planters Cooperative Union has increased from 156 tonnes in 2006 to over 400 tonnes in 2009. The increase in coffee exports was not by mere chance but a number of factors contributed to this success. The main reasons for this success were: • Strong prior relationship between the growers and the buyers • The uniqueness of the cooperative business model • The unique taste and quality of coffee grown • The introduction of a different approach to farming coffee Currently there is high demand for organic coffee and this could be a niche market that is not only rewarded with premium prices but also creates environmental friendly business. The union considers Fair Trade market as an alternative approach to conventional trade, aiming at improving the livelihoods of smallholder producers by improving payments, giving them fair prices and providing continuity in trading relationships.
- Full Text:
An evaluation of the quality customer service delivered by Eskom to rural household customers in the Eastern Cape
- Authors: Myoli, Mncedi Eric
- Date: 2011
- Subjects: Eskom (Firm) , Electric power distribution -- South Africa -- Eastern Cape , Electric power distribution -- Customer services -- South Africa -- Eastern Cape , Electric power distribution -- South Africa -- Eastern Cape -- Quality control , Consumer satisfaction -- South Africa -- Eastern Cape -- Evaluation , Customer services -- Rating of -- South Africa -- Eastern Cape , Rural electrification -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:838 , http://hdl.handle.net/10962/d1013564
- Description: [Abstract - Conclusion]: This research study confirms the theory in the literature review on delivery of quality service to customers and premise that there is a statistically significant difference between Eskom service as perceived by rural prepaid household customers and their expectations. This study also highlights the need for Eskom management to take into cognisance the unique rural prepaid customer service needs and how to meet them, perhaps a differentiated service approach rather than a one-strategy-fits-all approach. This is in view of gaps created between expectations and perceptions of service, especially in the reliability dimension that could require a concentrated or changed performance approach to close them.
- Full Text:
- Authors: Myoli, Mncedi Eric
- Date: 2011
- Subjects: Eskom (Firm) , Electric power distribution -- South Africa -- Eastern Cape , Electric power distribution -- Customer services -- South Africa -- Eastern Cape , Electric power distribution -- South Africa -- Eastern Cape -- Quality control , Consumer satisfaction -- South Africa -- Eastern Cape -- Evaluation , Customer services -- Rating of -- South Africa -- Eastern Cape , Rural electrification -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:838 , http://hdl.handle.net/10962/d1013564
- Description: [Abstract - Conclusion]: This research study confirms the theory in the literature review on delivery of quality service to customers and premise that there is a statistically significant difference between Eskom service as perceived by rural prepaid household customers and their expectations. This study also highlights the need for Eskom management to take into cognisance the unique rural prepaid customer service needs and how to meet them, perhaps a differentiated service approach rather than a one-strategy-fits-all approach. This is in view of gaps created between expectations and perceptions of service, especially in the reliability dimension that could require a concentrated or changed performance approach to close them.
- Full Text:
An interpretative phenomenological analysis of the experiences of HIV-positive lay counsellors working in the voluntary counselling and testing settings
- Authors: Teng, James Wei Jie
- Date: 2011
- Subjects: HIV-positive persons Peer counseling Health counseling Health counselors -- Mental health Hiv-positive persons -- Counseling of HIV-positive persons -- Mental health HIV infections -- Psychological aspects Lay analysis (Psychoanalysis)
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3070 , http://hdl.handle.net/10962/d1002579
- Description: The purpose of this study was to present and understand the experiences of HIV-positive lay counsellors working in Voluntary Counselling and Testing (VCT) settings. Specifically exploring and understanding the utilisation of personal experiences within counselling encounters, the practice of peer counselling within VCT, and the challenges experienced by HIV-positive lay counsellors within VCT settings. This study, employing a qualitative interpretative phenomenological methodology required a small sample of practicing HIV-positive lay counsellors, who were selected and interviewed on their experiences utilising semi-structured interviewing. Data was analysed for meaning units, which were interpreted inductively and hermeneutically, and categorised into super-ordinate themes. Three superordinate themes within the participants’ experiences of providing VCT services were determined, namely: ‘diagnosis and disclosure experiences’, ‘peer counselling’, and ‘challenges’. This research found that the experiences of providing peer counselling depended upon identification with their client’s negative appraisal of their diagnosis experiences. Whether through empathic connections generated through the shared experience of discovering a seropositive status, or through countertransferential reactions induced through their client’s yearning for care and support. This required the counsellor to selfdisclose within counselling encounters in order to provide personal experiences of living with HIV/AIDS. Successful implementation of peer counselling provided recently diagnosed individuals with knowledge surrounding HIV/AIDS, coping skills to manage the daily physiological and psychological challenges, facilitation and adherence to treatment, social assistance, ongoing relationships, inspiring hope, and the creation of positive appraisals. However the informal utilisation of task-shifting within lay healthcare cadres, and the lack of governmental recognition for the emotional labour provided within VCT indicated that HIVpositive lay counsellors require ongoing training, support and remuneration to limit potential occupational stress, resignation, and burnout.
- Full Text:
- Authors: Teng, James Wei Jie
- Date: 2011
- Subjects: HIV-positive persons Peer counseling Health counseling Health counselors -- Mental health Hiv-positive persons -- Counseling of HIV-positive persons -- Mental health HIV infections -- Psychological aspects Lay analysis (Psychoanalysis)
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3070 , http://hdl.handle.net/10962/d1002579
- Description: The purpose of this study was to present and understand the experiences of HIV-positive lay counsellors working in Voluntary Counselling and Testing (VCT) settings. Specifically exploring and understanding the utilisation of personal experiences within counselling encounters, the practice of peer counselling within VCT, and the challenges experienced by HIV-positive lay counsellors within VCT settings. This study, employing a qualitative interpretative phenomenological methodology required a small sample of practicing HIV-positive lay counsellors, who were selected and interviewed on their experiences utilising semi-structured interviewing. Data was analysed for meaning units, which were interpreted inductively and hermeneutically, and categorised into super-ordinate themes. Three superordinate themes within the participants’ experiences of providing VCT services were determined, namely: ‘diagnosis and disclosure experiences’, ‘peer counselling’, and ‘challenges’. This research found that the experiences of providing peer counselling depended upon identification with their client’s negative appraisal of their diagnosis experiences. Whether through empathic connections generated through the shared experience of discovering a seropositive status, or through countertransferential reactions induced through their client’s yearning for care and support. This required the counsellor to selfdisclose within counselling encounters in order to provide personal experiences of living with HIV/AIDS. Successful implementation of peer counselling provided recently diagnosed individuals with knowledge surrounding HIV/AIDS, coping skills to manage the daily physiological and psychological challenges, facilitation and adherence to treatment, social assistance, ongoing relationships, inspiring hope, and the creation of positive appraisals. However the informal utilisation of task-shifting within lay healthcare cadres, and the lack of governmental recognition for the emotional labour provided within VCT indicated that HIVpositive lay counsellors require ongoing training, support and remuneration to limit potential occupational stress, resignation, and burnout.
- Full Text:
An investigation into Grade R teachers' experiences of implementing numeracy in Grade R
- Authors: Barnard, Elna
- Date: 2011
- Subjects: Teachers -- South Africa -- Attitudes Numeracy -- Study and teaching (Elementary) -- South Africa Teachers -- Training of -- South Africa Mathematics teachers -- In-service training -- South Africa Early childhood education -- South Africa -- History Early childhood education -- South Africa -- Political aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1855 , http://hdl.handle.net/10962/d1004529
- Description: This research study investigates how selected Grade R teachers implement numeracy in their classrooms, based on their experiences, personal beliefs and perceptions. This study adopts the view that a teacher's practice is not only informed on what she believes about her learners, but also on how she organises her classroom environment and her own teaching practice. However, the teacher cannot be divorced from the political and historical background of Early Childhood Development (ECD), as these factors have an impact on her numeracy implementation. Integrated in this research study is thus not only a comprehensive historical analysis of the historical and political background of ECD, but also an in-depth look at the complex curriculum road the Grade R teacher had to travel over the past twelve years. The aim of this case study is to understand and describe what is happening in Grade R classrooms when numeracy is implemented, as well as identifying possible barriers which Grade R teachers may experience in implementing numeracy. The author utilized an adapted Interactive Qualitative Analysis Framework to explore Grade R teachers' views and experiences. Open-ended focus group interviews were used to develop a framework for individual interviews. This interview framework guided the data collection of nine audio-video tapes of classroom activities and nine semi-structured individual interviews. Analysis of the individual interviews revealed each teacher's understanding as well as her perceptions and needs regarding the implementation of numeracy in her classroom. The transcriptions of the individual interviews were compared to the audio-video tapes of what happened in each classroom. This was done in order to determine whether the teachers' practices were consistent with what they said in the interviews. Paradoxes and inconsistencies were documented. The historical and political analysis of ECD illustrates the complex development of ECD in South Africa over the last few decades. It therefore also highlights and brings to the fore the complex journey that Grade R teachers had to navigate in adapting their practice to ever changing curriculum requirements. The empirical results show that there are inconsistencies between what selected teachers know and believe, and what they are implementing. However, "silent themes" which did not come to the fore in the focus group or individual interviews, were identified in the audiovideo tapes of classroom activities. Also, many "silent themes" indicate that there are numerous concerns, such as lack of proficiency in bridging the gap between theory and practice, regarding the implementation of numeracy in Grade R. It was found that many of the selected Grade R teachers struggle to implement numeracy in their classrooms, as they lack the skills and support to teach numeracy in a Grade R context.
- Full Text:
- Authors: Barnard, Elna
- Date: 2011
- Subjects: Teachers -- South Africa -- Attitudes Numeracy -- Study and teaching (Elementary) -- South Africa Teachers -- Training of -- South Africa Mathematics teachers -- In-service training -- South Africa Early childhood education -- South Africa -- History Early childhood education -- South Africa -- Political aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1855 , http://hdl.handle.net/10962/d1004529
- Description: This research study investigates how selected Grade R teachers implement numeracy in their classrooms, based on their experiences, personal beliefs and perceptions. This study adopts the view that a teacher's practice is not only informed on what she believes about her learners, but also on how she organises her classroom environment and her own teaching practice. However, the teacher cannot be divorced from the political and historical background of Early Childhood Development (ECD), as these factors have an impact on her numeracy implementation. Integrated in this research study is thus not only a comprehensive historical analysis of the historical and political background of ECD, but also an in-depth look at the complex curriculum road the Grade R teacher had to travel over the past twelve years. The aim of this case study is to understand and describe what is happening in Grade R classrooms when numeracy is implemented, as well as identifying possible barriers which Grade R teachers may experience in implementing numeracy. The author utilized an adapted Interactive Qualitative Analysis Framework to explore Grade R teachers' views and experiences. Open-ended focus group interviews were used to develop a framework for individual interviews. This interview framework guided the data collection of nine audio-video tapes of classroom activities and nine semi-structured individual interviews. Analysis of the individual interviews revealed each teacher's understanding as well as her perceptions and needs regarding the implementation of numeracy in her classroom. The transcriptions of the individual interviews were compared to the audio-video tapes of what happened in each classroom. This was done in order to determine whether the teachers' practices were consistent with what they said in the interviews. Paradoxes and inconsistencies were documented. The historical and political analysis of ECD illustrates the complex development of ECD in South Africa over the last few decades. It therefore also highlights and brings to the fore the complex journey that Grade R teachers had to navigate in adapting their practice to ever changing curriculum requirements. The empirical results show that there are inconsistencies between what selected teachers know and believe, and what they are implementing. However, "silent themes" which did not come to the fore in the focus group or individual interviews, were identified in the audiovideo tapes of classroom activities. Also, many "silent themes" indicate that there are numerous concerns, such as lack of proficiency in bridging the gap between theory and practice, regarding the implementation of numeracy in Grade R. It was found that many of the selected Grade R teachers struggle to implement numeracy in their classrooms, as they lack the skills and support to teach numeracy in a Grade R context.
- Full Text:
An investigation into international transfer pricing guidelines and the anomalies arising from business restructurings by multi-national enterprises
- Authors: Stelloh, Marcus Matthias
- Date: 2011
- Language: English
- Type: text , Thesis , Masters , MCom
- Identifier: vital:879 , http://hdl.handle.net/10962/d1001633
- Description: The number of multinational enterprises has increased substantially. In part due to the integration of national economies (the European Union), improvements in communication and technology and the opportunity to reduce costs as a result of globalisation. Transfer pricing and especially business restructuring within multinationals is a fairly new concept.Professional legal and audit firms have different views on how to approach business restructurings. This research analyses important transfer pricing aspects and the anomalies that arise through business restructurings. The research method used in this research paper is primarily qualitative, comprising the analysis of various documentary sources of data. Relevant South African and international case law, tax legislation, the OECD Transfer Pricing Guidelines for Multinational Enterprises and Tax Administrations, the Transfer Pricing Aspects of Business Restructurings Discussion Draft and other reports were consulted and analysed. Further the views of recognised legal and tax experts that have been published in technical journals and text books were also considered and examined. A hypothetical example of a business restructuring transaction was constructed in order to illustrate practical issues and different approaches to solving them. The research has argued that the arm’s length principle, which forms the bases of transfer pricing regulation, is not an exact science but theoretically it is the most suitable measure.It may not be able to incorporate all variables, such as the cost savings through synergies of multinational enterprises, but it promotes international trade and investment by ensuring that transactions are based on fair prices. Business restructurings create anomalies in applying the arm’s length principle but these anomalies can be dealt with within the regulatory structure. The business restructuring approach recommended is realistic and pragmatic, but more clarity may be needed in certain circumstances. The research has also discussed the avoidance of transfer pricing audits, including having appropriate transfer pricing policies and documentation.
- Full Text:
- Authors: Stelloh, Marcus Matthias
- Date: 2011
- Language: English
- Type: text , Thesis , Masters , MCom
- Identifier: vital:879 , http://hdl.handle.net/10962/d1001633
- Description: The number of multinational enterprises has increased substantially. In part due to the integration of national economies (the European Union), improvements in communication and technology and the opportunity to reduce costs as a result of globalisation. Transfer pricing and especially business restructuring within multinationals is a fairly new concept.Professional legal and audit firms have different views on how to approach business restructurings. This research analyses important transfer pricing aspects and the anomalies that arise through business restructurings. The research method used in this research paper is primarily qualitative, comprising the analysis of various documentary sources of data. Relevant South African and international case law, tax legislation, the OECD Transfer Pricing Guidelines for Multinational Enterprises and Tax Administrations, the Transfer Pricing Aspects of Business Restructurings Discussion Draft and other reports were consulted and analysed. Further the views of recognised legal and tax experts that have been published in technical journals and text books were also considered and examined. A hypothetical example of a business restructuring transaction was constructed in order to illustrate practical issues and different approaches to solving them. The research has argued that the arm’s length principle, which forms the bases of transfer pricing regulation, is not an exact science but theoretically it is the most suitable measure.It may not be able to incorporate all variables, such as the cost savings through synergies of multinational enterprises, but it promotes international trade and investment by ensuring that transactions are based on fair prices. Business restructurings create anomalies in applying the arm’s length principle but these anomalies can be dealt with within the regulatory structure. The business restructuring approach recommended is realistic and pragmatic, but more clarity may be needed in certain circumstances. The research has also discussed the avoidance of transfer pricing audits, including having appropriate transfer pricing policies and documentation.
- Full Text:
An investigation into knowledge and change in a Grade 9 environmental research project
- Authors: Webber, Susan Marion
- Date: 2011
- Subjects: Environmental education -- Activity programs -- South Africa Environmental education -- Evaluation -- South Africa Environmental education -- Study and teaching (Secondary) -- South Africa Sustainable development -- Evaluation -- South Africa Sustainable development -- Study and teaching (Secondary) -- South Africa Active learning -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1597 , http://hdl.handle.net/10962/d1003479
- Description: This study considers a Grade 9 Integrated Environmental Research Project which was implemented as a vehicle to induce knowledge-based change in learners. It was noted that change did not occur as hoped, and this study was undertaken to review the Grade 9 Project in order to improve it and to probe the apparent gap between knowledge and action. The study generated evidence on the learning processes within the project. This revealed a number of contradictions and tensions which limit change initiatives within the local environment. Notable here was a contradictory mandate between undertaking a research-based change project and responding to the rubric of assessment which was not linked to the research done. It was found that faced with this dual mandate, learners chose to focus on the assessment-laden mandate as this was the ultimate agenda that would reap the reward within the traditional school environment. The study examines the gap between knowledge and practice to probe ways in which to close this gap in the context of an environmental research assignment. The outcome is a recommendation that we as the project designers review the evidence of tensions and contradictions revealed in the study to reflect on the underlying purpose of the project and reshape it in light of recent literature on the challenges of social learning and change.
- Full Text:
- Authors: Webber, Susan Marion
- Date: 2011
- Subjects: Environmental education -- Activity programs -- South Africa Environmental education -- Evaluation -- South Africa Environmental education -- Study and teaching (Secondary) -- South Africa Sustainable development -- Evaluation -- South Africa Sustainable development -- Study and teaching (Secondary) -- South Africa Active learning -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1597 , http://hdl.handle.net/10962/d1003479
- Description: This study considers a Grade 9 Integrated Environmental Research Project which was implemented as a vehicle to induce knowledge-based change in learners. It was noted that change did not occur as hoped, and this study was undertaken to review the Grade 9 Project in order to improve it and to probe the apparent gap between knowledge and action. The study generated evidence on the learning processes within the project. This revealed a number of contradictions and tensions which limit change initiatives within the local environment. Notable here was a contradictory mandate between undertaking a research-based change project and responding to the rubric of assessment which was not linked to the research done. It was found that faced with this dual mandate, learners chose to focus on the assessment-laden mandate as this was the ultimate agenda that would reap the reward within the traditional school environment. The study examines the gap between knowledge and practice to probe ways in which to close this gap in the context of an environmental research assignment. The outcome is a recommendation that we as the project designers review the evidence of tensions and contradictions revealed in the study to reflect on the underlying purpose of the project and reshape it in light of recent literature on the challenges of social learning and change.
- Full Text:
An investigation into the replication biology of Helicoverpa armigera stunt virus
- Authors: Short, James Roswell
- Date: 2011
- Subjects: Helicoverpa armigera RNA viruses Viruses -- Reproduction Lepidoptera -- Viruses
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3967 , http://hdl.handle.net/10962/d1004026
- Description: Tetraviruses are a family of small non-enveloped positive sense RNA viruses that exclusively infect members of the order Lepidoptera. Their replication biology is poorly studied because, with the exception of Providence virus (PrV), tetraviruses are unable to replicate in tissue culture cells. The overall aim of the research described in this thesis was to develop a fundamental understanding of the replication of tetraviruses, focussing on the site of replication within host cells and in particular, the subcellular localisation of the viral replicase. Helicoverpa armigera stunt virus (HaSV, Genus: Omegatetravirus) was chosen for this study because it is the only tetravirus for which the cDNAs have been shown to be infectious. In the absence of tissue culture cell lines susceptible to HaSV infection, the approach was to use confocal fluorescence microscopy to examine the subcellular localisation of the HaSV replicase fused to enhanced green fluorescent protein (EGFP) in mammalian and insect tissue culture cells. The replicase (with EGFP fused at its C-terminus) localised to punctate structures throughout the cytoplasm of transfected HeLa and Sf9 cells. These structures were then shown – using live cell imaging and time lapse photography – to behave similarly to cellular endocytic organelles and fluorescence partially overlapped with membranes containing the late endosomal marker protein CD63. Biochemical fractionation of Sf9 cells expressing the replicase via a recombinant baculovirus (as well as transfected HeLa and Sf9 cells expressing EGFP-replicase fusion proteins) demonstrated that the replicase was strongly associated with detergentresistant membranes (DRMs) in these cells. Deletion analysis of the replicase coding sequence revealed two regions involved in the generation of the punctuate structures. Firstly, the C-terminal half of the replicase RNAdependant RNA polymerase domain was found to be essential for targeting and the tight association with DRMs while the second region, within the Nterminal 44 amino acids, enhanced localisation through a combination of secondary structural elements and sequence-specific functions. A comparative immunofluorescence study on PrV, which replicates as a persistent infection in an insect midgut cell line, showed that the PrV replicase also localised to punctate structures in the cytoplasm. Biochemical fractionation showed that the replicase was also strongly associated with DRMs. This thesis describes the development of new experimental systems for the study of tetravirus replication biology and the data lead to the conclusion that the HaSV replicase associates with DRMs derived from alternate endocytic pathway organelles.
- Full Text:
- Authors: Short, James Roswell
- Date: 2011
- Subjects: Helicoverpa armigera RNA viruses Viruses -- Reproduction Lepidoptera -- Viruses
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3967 , http://hdl.handle.net/10962/d1004026
- Description: Tetraviruses are a family of small non-enveloped positive sense RNA viruses that exclusively infect members of the order Lepidoptera. Their replication biology is poorly studied because, with the exception of Providence virus (PrV), tetraviruses are unable to replicate in tissue culture cells. The overall aim of the research described in this thesis was to develop a fundamental understanding of the replication of tetraviruses, focussing on the site of replication within host cells and in particular, the subcellular localisation of the viral replicase. Helicoverpa armigera stunt virus (HaSV, Genus: Omegatetravirus) was chosen for this study because it is the only tetravirus for which the cDNAs have been shown to be infectious. In the absence of tissue culture cell lines susceptible to HaSV infection, the approach was to use confocal fluorescence microscopy to examine the subcellular localisation of the HaSV replicase fused to enhanced green fluorescent protein (EGFP) in mammalian and insect tissue culture cells. The replicase (with EGFP fused at its C-terminus) localised to punctate structures throughout the cytoplasm of transfected HeLa and Sf9 cells. These structures were then shown – using live cell imaging and time lapse photography – to behave similarly to cellular endocytic organelles and fluorescence partially overlapped with membranes containing the late endosomal marker protein CD63. Biochemical fractionation of Sf9 cells expressing the replicase via a recombinant baculovirus (as well as transfected HeLa and Sf9 cells expressing EGFP-replicase fusion proteins) demonstrated that the replicase was strongly associated with detergentresistant membranes (DRMs) in these cells. Deletion analysis of the replicase coding sequence revealed two regions involved in the generation of the punctuate structures. Firstly, the C-terminal half of the replicase RNAdependant RNA polymerase domain was found to be essential for targeting and the tight association with DRMs while the second region, within the Nterminal 44 amino acids, enhanced localisation through a combination of secondary structural elements and sequence-specific functions. A comparative immunofluorescence study on PrV, which replicates as a persistent infection in an insect midgut cell line, showed that the PrV replicase also localised to punctate structures in the cytoplasm. Biochemical fractionation showed that the replicase was also strongly associated with DRMs. This thesis describes the development of new experimental systems for the study of tetravirus replication biology and the data lead to the conclusion that the HaSV replicase associates with DRMs derived from alternate endocytic pathway organelles.
- Full Text: