Responding to poverty in the light of the post-development debate: Some insights from the NGO Enda Graf Sahel
- Authors: Matthews, Sally
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142487 , vital:38084 , https://www.ajol.info/index.php/ad/article/view/135799
- Description: How can we take on board the many valuable insights of post-development theory without seeming to advocate indifference and inaction in the face of the misery that many people in the world experience daily? In this paper, I provide a partial response to this question. I begin by looking at some of the alternative strategies offered in post-development literature and set out to show that while there are several problems with these alternatives, to read post-development theory as advocating indifference or inaction is to read it uncharitably. Secondly, I draw on the experiences of the non-governmental organisation (NGO) Enda Graf Sahel in Dakar, Senegal to suggest some ways in which the insights of post-development theory, or some versions of post-development theory, can be taken into consideration without leading to inaction or indifference in the face of the suffering of those who occupy a less advantaged position in contemporary relations of power and privilege.
- Full Text:
- Date Issued: 2016
- Authors: Matthews, Sally
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142487 , vital:38084 , https://www.ajol.info/index.php/ad/article/view/135799
- Description: How can we take on board the many valuable insights of post-development theory without seeming to advocate indifference and inaction in the face of the misery that many people in the world experience daily? In this paper, I provide a partial response to this question. I begin by looking at some of the alternative strategies offered in post-development literature and set out to show that while there are several problems with these alternatives, to read post-development theory as advocating indifference or inaction is to read it uncharitably. Secondly, I draw on the experiences of the non-governmental organisation (NGO) Enda Graf Sahel in Dakar, Senegal to suggest some ways in which the insights of post-development theory, or some versions of post-development theory, can be taken into consideration without leading to inaction or indifference in the face of the suffering of those who occupy a less advantaged position in contemporary relations of power and privilege.
- Full Text:
- Date Issued: 2016
Reviewing strategies in/for ESD policy engagement: Agency reclaimed
- Authors: Lotz-Sisitka, Heila
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/182483 , vital:43834 , xlink:href="https://doi.org/10.1080/00958964.2015.1113915"
- Description: In this response article, I draw on critical realist perspectives to engage with the argument put forward in Bengtsson's study, which sees agency as an ontological necessity for Education for Sustainable Development (ESD) policy engagement. Bengtsson supports a notion of the logic of contingent action over the logic of power as dominance, suggesting possibilities for agency and resistance. Although I do not in principle disagree with the agentive possibilities embedded in this aspect of the Bengtsson argument, it is the scope of the conceptualization thereof that I consider in this response. I start with considering the limitations of a Westphalian analysis of policy appropriations and agency for ESD, and argue that the Westphalian frame for policy analysis may be inadequate for capturing the significance of non-state actors and wider generative mechanisms such as informal normative structures, and private, economic power in the global political economy. Drawing on Fraser's (2008) concept of the transnational public sphere, I explore other potential possibilities for agency-centered appropriations or negations of, and/or resistance to ESD policy discourses, potentially expanding the agency-centered perspective referred to in Bengtsson's analysis and critique of policy making for ESD, or, at the very least, by offering a wider view of possibility for what he refers to as the ‘ineradicable moment of conflict, or antagonism.’ In particular, I broaden the notion of the transnational public sphere to be inclusive of Dussel's (1998) three concerns of transformation, namely; poverty and wealth inequality, environmental degradation, and narrow rationalities involving ongoing colonization of people, territories and resources. In doing this, I concur with Fraser, who suggests that the concept of the public sphere may well be “so thoroughly Westphalian in its deep conceptual structure as to be unsalvageable as a critical tool for theorizing the present” and suggest that public sphere thinking and associated conceptions of agency require expansion, which I offer from postcolonial and decolonization literature, critical realism, ontological experiences, and reflection on Environmental Education (EE) /ESD policy in the southern African region. Ultimately, I propose need for a more radical framework for EE/ ESD policy research that reaches beyond analyses of appropriations of policy within the Wesphalian state framework, and that moves beyond critiquing or seeking out resistance moments associated with the assumptions of trickle down effects from UN level policy, or analysis that is centered on the EE versus ESD debate. Such a framework requires a revitalized notion of agency involving commitment to collective, relational (including the socio-materially relational) and transgressive forms of agency for deep societal transformations all round. Overall, it seems that environmental education policy and praxis research conceptualized within a decolonizing transnational sphere frame appears to still be an open and as yet under-explored terrain.
- Full Text:
- Date Issued: 2016
- Authors: Lotz-Sisitka, Heila
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/182483 , vital:43834 , xlink:href="https://doi.org/10.1080/00958964.2015.1113915"
- Description: In this response article, I draw on critical realist perspectives to engage with the argument put forward in Bengtsson's study, which sees agency as an ontological necessity for Education for Sustainable Development (ESD) policy engagement. Bengtsson supports a notion of the logic of contingent action over the logic of power as dominance, suggesting possibilities for agency and resistance. Although I do not in principle disagree with the agentive possibilities embedded in this aspect of the Bengtsson argument, it is the scope of the conceptualization thereof that I consider in this response. I start with considering the limitations of a Westphalian analysis of policy appropriations and agency for ESD, and argue that the Westphalian frame for policy analysis may be inadequate for capturing the significance of non-state actors and wider generative mechanisms such as informal normative structures, and private, economic power in the global political economy. Drawing on Fraser's (2008) concept of the transnational public sphere, I explore other potential possibilities for agency-centered appropriations or negations of, and/or resistance to ESD policy discourses, potentially expanding the agency-centered perspective referred to in Bengtsson's analysis and critique of policy making for ESD, or, at the very least, by offering a wider view of possibility for what he refers to as the ‘ineradicable moment of conflict, or antagonism.’ In particular, I broaden the notion of the transnational public sphere to be inclusive of Dussel's (1998) three concerns of transformation, namely; poverty and wealth inequality, environmental degradation, and narrow rationalities involving ongoing colonization of people, territories and resources. In doing this, I concur with Fraser, who suggests that the concept of the public sphere may well be “so thoroughly Westphalian in its deep conceptual structure as to be unsalvageable as a critical tool for theorizing the present” and suggest that public sphere thinking and associated conceptions of agency require expansion, which I offer from postcolonial and decolonization literature, critical realism, ontological experiences, and reflection on Environmental Education (EE) /ESD policy in the southern African region. Ultimately, I propose need for a more radical framework for EE/ ESD policy research that reaches beyond analyses of appropriations of policy within the Wesphalian state framework, and that moves beyond critiquing or seeking out resistance moments associated with the assumptions of trickle down effects from UN level policy, or analysis that is centered on the EE versus ESD debate. Such a framework requires a revitalized notion of agency involving commitment to collective, relational (including the socio-materially relational) and transgressive forms of agency for deep societal transformations all round. Overall, it seems that environmental education policy and praxis research conceptualized within a decolonizing transnational sphere frame appears to still be an open and as yet under-explored terrain.
- Full Text:
- Date Issued: 2016
Revisiting cellulase production and redefining current strategies based on major challenges
- Kuhad, Ramesh Chander, Deswal, Deepa, Sharma, Sonia, Bhattacharya, Abhishek, Jain, Kavish Kumar, Kaur, Amandeep, Pletschke, Brett I, Singh, Ajay, Karp, Matti
- Authors: Kuhad, Ramesh Chander , Deswal, Deepa , Sharma, Sonia , Bhattacharya, Abhishek , Jain, Kavish Kumar , Kaur, Amandeep , Pletschke, Brett I , Singh, Ajay , Karp, Matti
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66142 , vital:28909 , https://doi.org/10.1016/j.rser.2015.10.132
- Description: publisher version , Lignocellulosic biomass has been considered as an important and sustainable source of renewable energy. Cellulose constitutes the major component of the lignocellulosic biomass and also offers maximum recalcitrance towards its fullest utilization. The enzymatic breakdown of cellulose is achieved through cellulases. Diverse forms of microbes including fungi, bacteria, actinomycetes and yeast are known to produce cellulases that have found extensive application in various industries. Due to the current global political unrest over oil prices and the threat of global warming following combustion of fossil fuels, the paradigm of research is now focused on biofuel production from plant biomass. Conventional approaches have not been economically feasible for meeting the demands of the industry. This review provides an update regarding the status of present microbial cellulase production technologies and research with special reference to solid state fermentation and different molecular techniques such as mutagenesis, metabolic engineering and heterologous gene expression of cellulases from different microbial domains with improved catalytic and stability properties. Metagenomic and genomic studies for mining of novel cellulase genes in addition to screening of culturable strains using conventional methods have been advanced. In addition the bottlenecks associated with cellulase production and how the future research needs to be directed to provide a comprehensive technology for the production of cellulases with novel traits for application at an industrial level without economic constraints are discussed.
- Full Text: false
- Date Issued: 2016
- Authors: Kuhad, Ramesh Chander , Deswal, Deepa , Sharma, Sonia , Bhattacharya, Abhishek , Jain, Kavish Kumar , Kaur, Amandeep , Pletschke, Brett I , Singh, Ajay , Karp, Matti
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66142 , vital:28909 , https://doi.org/10.1016/j.rser.2015.10.132
- Description: publisher version , Lignocellulosic biomass has been considered as an important and sustainable source of renewable energy. Cellulose constitutes the major component of the lignocellulosic biomass and also offers maximum recalcitrance towards its fullest utilization. The enzymatic breakdown of cellulose is achieved through cellulases. Diverse forms of microbes including fungi, bacteria, actinomycetes and yeast are known to produce cellulases that have found extensive application in various industries. Due to the current global political unrest over oil prices and the threat of global warming following combustion of fossil fuels, the paradigm of research is now focused on biofuel production from plant biomass. Conventional approaches have not been economically feasible for meeting the demands of the industry. This review provides an update regarding the status of present microbial cellulase production technologies and research with special reference to solid state fermentation and different molecular techniques such as mutagenesis, metabolic engineering and heterologous gene expression of cellulases from different microbial domains with improved catalytic and stability properties. Metagenomic and genomic studies for mining of novel cellulase genes in addition to screening of culturable strains using conventional methods have been advanced. In addition the bottlenecks associated with cellulase production and how the future research needs to be directed to provide a comprehensive technology for the production of cellulases with novel traits for application at an industrial level without economic constraints are discussed.
- Full Text: false
- Date Issued: 2016
Reflections on Teaching Africa in South Africa:
- Authors: Matthews, Sally
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142235 , vital:38061 , DOI: 10.1111/1467-9256.12107
- Description: This article draws on the author’s experience of teaching African Studies to undergraduate South African students in order to reflect on some of the key challenges facing teachers of African Studies, both in South Africa and beyond. In particular, it discusses challenges relating to teaching a field as contested as African Studies, looking at whether teaching African alternatives to mainstream African politics is helpful and at whether and how one can teach Africa in a way that encourages and develops critical thinking. The article also explores how the racial politics of the context in which one teaches African Studies inevitably affects the way in which students engage with the content of the course. While the article discusses these issues in relation to the South African higher education context in particular, implications for other contexts are also highlighted.
- Full Text:
- Date Issued: 2016
- Authors: Matthews, Sally
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142235 , vital:38061 , DOI: 10.1111/1467-9256.12107
- Description: This article draws on the author’s experience of teaching African Studies to undergraduate South African students in order to reflect on some of the key challenges facing teachers of African Studies, both in South Africa and beyond. In particular, it discusses challenges relating to teaching a field as contested as African Studies, looking at whether teaching African alternatives to mainstream African politics is helpful and at whether and how one can teach Africa in a way that encourages and develops critical thinking. The article also explores how the racial politics of the context in which one teaches African Studies inevitably affects the way in which students engage with the content of the course. While the article discusses these issues in relation to the South African higher education context in particular, implications for other contexts are also highlighted.
- Full Text:
- Date Issued: 2016
Russian wheat aphids: Breakfast, lunch, and supper. Feasting on small grains in South Africa
- Botha, Christiaan E J, Sacranie, S, Gallagher, Sean, Hill, Jaclyn M
- Authors: Botha, Christiaan E J , Sacranie, S , Gallagher, Sean , Hill, Jaclyn M
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/69031 , vital:29374 , https://doi.org/10.1016/j.sajb.2016.12.006
- Description: The Russian Wheat Aphid (Diuraphis noxia, RWA) negatively impacts commercially grown barley and wheat in South Africa. Climate change, the attendant rise in [CO2], and the appearance of new RWA biotypes have the potential to induce severe crop yield loss in agriculturally important wheat and barley cultivars. This study presents data showing changes in relative aphid population numbers, concurrently with assessments of plant damage under controlled environmental conditions, under ambient and elevated (450 ppm) [CO2]. Extensive structural damage to the vascular tissue and disruption to the transport systems were revealed using light, fluorescence and electron microscopy. This, coupled with biotype population studies, demonstrated that RWA has the capacity to inflict severe, potentially permanent damage to vegetative small grain plants. Furthermore, some currently ‘resistant’ cultivars may well lose resistance as a direct result of increasing atmospheric [CO2]. A small (50 ppm) increase in atmospheric [CO2] may result in increased aphid population numbers, potentially serious plant damage and, by implication, a potentially negative impact on yield, as increased aphid density per plant leads to an accelerated disruption of the assimilate and transpiration transport pathways. These outcomes pose a direct threat to the commercial small grain industry of South Africa and by extension, to other small grain production areas elsewhere.
- Full Text: false
- Date Issued: 2016
- Authors: Botha, Christiaan E J , Sacranie, S , Gallagher, Sean , Hill, Jaclyn M
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/69031 , vital:29374 , https://doi.org/10.1016/j.sajb.2016.12.006
- Description: The Russian Wheat Aphid (Diuraphis noxia, RWA) negatively impacts commercially grown barley and wheat in South Africa. Climate change, the attendant rise in [CO2], and the appearance of new RWA biotypes have the potential to induce severe crop yield loss in agriculturally important wheat and barley cultivars. This study presents data showing changes in relative aphid population numbers, concurrently with assessments of plant damage under controlled environmental conditions, under ambient and elevated (450 ppm) [CO2]. Extensive structural damage to the vascular tissue and disruption to the transport systems were revealed using light, fluorescence and electron microscopy. This, coupled with biotype population studies, demonstrated that RWA has the capacity to inflict severe, potentially permanent damage to vegetative small grain plants. Furthermore, some currently ‘resistant’ cultivars may well lose resistance as a direct result of increasing atmospheric [CO2]. A small (50 ppm) increase in atmospheric [CO2] may result in increased aphid population numbers, potentially serious plant damage and, by implication, a potentially negative impact on yield, as increased aphid density per plant leads to an accelerated disruption of the assimilate and transpiration transport pathways. These outcomes pose a direct threat to the commercial small grain industry of South Africa and by extension, to other small grain production areas elsewhere.
- Full Text: false
- Date Issued: 2016
Sarqaquinoic acid and related synthetic naphthoquinones inhibit the function of Hsp90
- Chiwakata, M, de la Mare, Jo-Anne, Edkins, Adrienne L, Beukes, Denzil R
- Authors: Chiwakata, M , de la Mare, Jo-Anne , Edkins, Adrienne L , Beukes, Denzil R
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66324 , vital:28933 , https://doi.org/10.1055/s-0036-1596751
- Description: publisher version , Heat shock protein 90 (Hsp90) is of critical importance in the proper folding of numerous proteins, including those involved in cancer. Consequently, there is significant interest in the discovery and development of Hsp90 inhibitors as anticancer drugs. In this study, we investigated the ability of sargaquinoic acid (SQA) and selected naphthoquinone derivatives to inhibit Hsp90 function. SQA was isolated and purified from Sargassum incisifolium while the naphthoquinones were synthesised via a straightforward sequence incorporating a Diels-Alder reaction between benzoquinone derivatives and myrcene followed by coupling with substituted alkyl or arylamines. Hsp90 inhibition was assessed by a client protein degradation assay. At a concentration of 1µM, SQA showed almost complete inhibition of Hsp90 but only moderate antiproliferative effects (IC50 658µM) against a Hs578T breast cancer carcinoma cell line. Interestingly, the most potent synthetic aminonaphthoquinone inhibited Hsp90 function by 50% at a concentration of 1µM but showed much improved activity against the Hs578T cell line (IC50 0.32µM). Furthermore, unlike geldanamycin, none of the compounds tested upregulates Hsp70 suggesting that these compounds may bind to the C-terminal end of Hsp90.
- Full Text: false
- Date Issued: 2016
- Authors: Chiwakata, M , de la Mare, Jo-Anne , Edkins, Adrienne L , Beukes, Denzil R
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66324 , vital:28933 , https://doi.org/10.1055/s-0036-1596751
- Description: publisher version , Heat shock protein 90 (Hsp90) is of critical importance in the proper folding of numerous proteins, including those involved in cancer. Consequently, there is significant interest in the discovery and development of Hsp90 inhibitors as anticancer drugs. In this study, we investigated the ability of sargaquinoic acid (SQA) and selected naphthoquinone derivatives to inhibit Hsp90 function. SQA was isolated and purified from Sargassum incisifolium while the naphthoquinones were synthesised via a straightforward sequence incorporating a Diels-Alder reaction between benzoquinone derivatives and myrcene followed by coupling with substituted alkyl or arylamines. Hsp90 inhibition was assessed by a client protein degradation assay. At a concentration of 1µM, SQA showed almost complete inhibition of Hsp90 but only moderate antiproliferative effects (IC50 658µM) against a Hs578T breast cancer carcinoma cell line. Interestingly, the most potent synthetic aminonaphthoquinone inhibited Hsp90 function by 50% at a concentration of 1µM but showed much improved activity against the Hs578T cell line (IC50 0.32µM). Furthermore, unlike geldanamycin, none of the compounds tested upregulates Hsp70 suggesting that these compounds may bind to the C-terminal end of Hsp90.
- Full Text: false
- Date Issued: 2016
Schools performing against the odds
- Niacker, Inbanathan, Grant, Carolyn, Pillay, Sivanandani
- Authors: Niacker, Inbanathan , Grant, Carolyn , Pillay, Sivanandani
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/281126 , vital:55694 , xlink:href="https://doi.org/10.15700/saje.v36n4a1321"
- Description: There are many schools in developing countries which, despite the challenges they face, defy the odds and continue to perform at exceptionally high levels. We cast our gaze on one of these resilient schools in South Africa, and sought to learn about the leadership practices prevalent in this school and the enablements and constraints to the school leadership practice. Underpinned by a critical realist lens, and drawing on social realist theory, this case study of one school generated data through interviews, observation, document analysis and transect walks. The school principal, one head of department and two teachers, were selected as participants. The findings indicate that the school embraced an expansive form of teacher leadership comprising leadership within and beyond the classroom. Further, the structural, cultural and agential climate was receptive to the expansive leadership. We conclude that the professional capital of teachers, together with teachers serving as social actors rather than remaining primary agents, are key resources to change and transformation in an emerging economy.
- Full Text:
- Date Issued: 2016
- Authors: Niacker, Inbanathan , Grant, Carolyn , Pillay, Sivanandani
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/281126 , vital:55694 , xlink:href="https://doi.org/10.15700/saje.v36n4a1321"
- Description: There are many schools in developing countries which, despite the challenges they face, defy the odds and continue to perform at exceptionally high levels. We cast our gaze on one of these resilient schools in South Africa, and sought to learn about the leadership practices prevalent in this school and the enablements and constraints to the school leadership practice. Underpinned by a critical realist lens, and drawing on social realist theory, this case study of one school generated data through interviews, observation, document analysis and transect walks. The school principal, one head of department and two teachers, were selected as participants. The findings indicate that the school embraced an expansive form of teacher leadership comprising leadership within and beyond the classroom. Further, the structural, cultural and agential climate was receptive to the expansive leadership. We conclude that the professional capital of teachers, together with teachers serving as social actors rather than remaining primary agents, are key resources to change and transformation in an emerging economy.
- Full Text:
- Date Issued: 2016
Screening of entomopathogenic fungi against citrus mealybug, Plannococcus citri (Hemiptera: Pseudococcidae)
- Chartier Fitzgerald, Veronique, Hill, Martin P, Moore, Sean D, Dames, Joanna F
- Authors: Chartier Fitzgerald, Veronique , Hill, Martin P , Moore, Sean D , Dames, Joanna F
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/407059 , vital:70333 , xlink:href="https://hdl.handle.net/10520/EJC195093"
- Description: Planococcus citri (citrus mealybug) is a common and damaging citrus crop pest which has proven difficult to control using conventional methods, such as chemical pesticides and insect growth regulators, particularly late in the citrus growing season. The virulence of two entomopathogenic fungal species was studied in laboratory bioassays against the crawlers and adults of P. citri. Isolates of Metarhizium anisopliae and Beauveria bassiana, collected from citrus orchards in the Eastern Cape Province in South Africa, were verified using and molecular techniques. Mealybug bioassays were performed in 24-well plates. Beauveria bassiana (GAR 17 B3) and M. anisopliae (FCM AR 23 B3) isolates both resulted in 67.5 % mortality of mealybug crawlers and B. bassiana (GB AR 23 13 3) resulted in 64 % crawler mortality with concentrations of 1 x 107 conidia/ml. These three isolates were further tested in multipledose bioassays to determine the median lethal concentration (LC50), which were 5.29 x 105conidia/ml for the M. anisopliae isolate (FCM AR 23 B3), 4.25 x 106 conidia/ml for B. bassiana (GAR 17 B3), and 6.65 x 107 conidia/ml B. bassiana (GB AR 23 13 3) for crawlers, respectively. The results of this study suggested that two isolates (M. anisopliae FCM AR 23 B3 and B. bassiana GAR 17 B3) showed potential for further development as biological control agents against citrus mealybug. Further research would be required to determine their ability to perform under field conditions.
- Full Text:
- Date Issued: 2016
- Authors: Chartier Fitzgerald, Veronique , Hill, Martin P , Moore, Sean D , Dames, Joanna F
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/407059 , vital:70333 , xlink:href="https://hdl.handle.net/10520/EJC195093"
- Description: Planococcus citri (citrus mealybug) is a common and damaging citrus crop pest which has proven difficult to control using conventional methods, such as chemical pesticides and insect growth regulators, particularly late in the citrus growing season. The virulence of two entomopathogenic fungal species was studied in laboratory bioassays against the crawlers and adults of P. citri. Isolates of Metarhizium anisopliae and Beauveria bassiana, collected from citrus orchards in the Eastern Cape Province in South Africa, were verified using and molecular techniques. Mealybug bioassays were performed in 24-well plates. Beauveria bassiana (GAR 17 B3) and M. anisopliae (FCM AR 23 B3) isolates both resulted in 67.5 % mortality of mealybug crawlers and B. bassiana (GB AR 23 13 3) resulted in 64 % crawler mortality with concentrations of 1 x 107 conidia/ml. These three isolates were further tested in multipledose bioassays to determine the median lethal concentration (LC50), which were 5.29 x 105conidia/ml for the M. anisopliae isolate (FCM AR 23 B3), 4.25 x 106 conidia/ml for B. bassiana (GAR 17 B3), and 6.65 x 107 conidia/ml B. bassiana (GB AR 23 13 3) for crawlers, respectively. The results of this study suggested that two isolates (M. anisopliae FCM AR 23 B3 and B. bassiana GAR 17 B3) showed potential for further development as biological control agents against citrus mealybug. Further research would be required to determine their ability to perform under field conditions.
- Full Text:
- Date Issued: 2016
SEAmester – South Africa’s first class afloat
- Ansorge, Isabelle J, Brundrit, Geoff, Brundrit, Jean, Dorrington, Rosemary A, Fawcett, Sarah, Gammon, David, Henry, Tahlia, Hermes, Juliet, Hölscher, Beate, d’Hotman, Jethan, Meiklejohn, Ian, Morris, Tammy, Pinto, Izidine, Du Plessis, Marcel, Roman, Raymond, Saunders, Clinton, Shabangu, Fannie W, De Vos, Marc, Walker, David R, Louw, Gavin
- Authors: Ansorge, Isabelle J , Brundrit, Geoff , Brundrit, Jean , Dorrington, Rosemary A , Fawcett, Sarah , Gammon, David , Henry, Tahlia , Hermes, Juliet , Hölscher, Beate , d’Hotman, Jethan , Meiklejohn, Ian , Morris, Tammy , Pinto, Izidine , Du Plessis, Marcel , Roman, Raymond , Saunders, Clinton , Shabangu, Fannie W , De Vos, Marc , Walker, David R , Louw, Gavin
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/65539 , vital:28808 , https://doi.org/10.17159/sajs.2016/a0171
- Description: publisher version , From Introduction: Marine science is a highly competitive environment. The need to improve the cohort of South African postgraduates, who would be recognised both nationally and internationally for their scientific excellence, is crucial. It is possible to attract students early on in their careers to this discipline via cutting-edge science, technology and unique field experiences. Through the engagement of students with real-life experiences such as SEAmester, universities supporting marine science postgraduate degree programmes can attract a sustainable throughput of numerically proficient students. By achieving a more quantitative and experienced input into our postgraduate degree programmes, we will, as a scientific community, greatly improve our long-term capabilities to accurately measure, model and predict the impacts of current climate change scenarios. The short-term goal is to attract and establish a cohort of proficient marine and atmospheric science graduates who will contribute to filling the capacity needs of South African marine science as a whole. The SEAmester programme, by involving researchers from across all the relevant disciplines and tertiary institutions, provides an opportunity to build a network of collaborative teaching within the marine field. In doing so, these researchers will foster and strengthen new and current collaborations between historically white and black universities (Figure 1). The long-term objective of SEAmester is to build critical mass within the marine sciences to ensure sustained growth of human capacity in marine science in South Africa – aligning closely with the current DST Research and Development strategies and the Operation Phakisa Oceans Economy initiative.
- Full Text:
- Date Issued: 2016
- Authors: Ansorge, Isabelle J , Brundrit, Geoff , Brundrit, Jean , Dorrington, Rosemary A , Fawcett, Sarah , Gammon, David , Henry, Tahlia , Hermes, Juliet , Hölscher, Beate , d’Hotman, Jethan , Meiklejohn, Ian , Morris, Tammy , Pinto, Izidine , Du Plessis, Marcel , Roman, Raymond , Saunders, Clinton , Shabangu, Fannie W , De Vos, Marc , Walker, David R , Louw, Gavin
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/65539 , vital:28808 , https://doi.org/10.17159/sajs.2016/a0171
- Description: publisher version , From Introduction: Marine science is a highly competitive environment. The need to improve the cohort of South African postgraduates, who would be recognised both nationally and internationally for their scientific excellence, is crucial. It is possible to attract students early on in their careers to this discipline via cutting-edge science, technology and unique field experiences. Through the engagement of students with real-life experiences such as SEAmester, universities supporting marine science postgraduate degree programmes can attract a sustainable throughput of numerically proficient students. By achieving a more quantitative and experienced input into our postgraduate degree programmes, we will, as a scientific community, greatly improve our long-term capabilities to accurately measure, model and predict the impacts of current climate change scenarios. The short-term goal is to attract and establish a cohort of proficient marine and atmospheric science graduates who will contribute to filling the capacity needs of South African marine science as a whole. The SEAmester programme, by involving researchers from across all the relevant disciplines and tertiary institutions, provides an opportunity to build a network of collaborative teaching within the marine field. In doing so, these researchers will foster and strengthen new and current collaborations between historically white and black universities (Figure 1). The long-term objective of SEAmester is to build critical mass within the marine sciences to ensure sustained growth of human capacity in marine science in South Africa – aligning closely with the current DST Research and Development strategies and the Operation Phakisa Oceans Economy initiative.
- Full Text:
- Date Issued: 2016
Sentential negation in South African Sign Language: A case study
- De Barros, Courtney, Siebörger, Ian
- Authors: De Barros, Courtney , Siebörger, Ian
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/385377 , vital:68013 , xlink:href=" https://hdl.handle.net/10520/EJC-4acb542a9"
- Description: As with other sign languages, South African Sign Language (SASL) expresses negation using both manual and non-manual features. In this case study, naturalistic data provided by two native signers of SASL are analysed to show the syntactic relationship between these two sets of features. Using a Principles and Parameters approach and Government and Binding Theory, we investigate the syntactic scope of negation in our SASL data. We observe that side-to-side headshake, as a non-manual feature, appears to be the chief clausal negator in SASL, with a clause-final manual negative particle, NOT, playing a secondary role. We describe the negative headshake as a featural affix which is base-generated in the head of NegP and triggers V-to- Neg raising. The negative particle NOT appears to be base-generated in the Specifier of NegP. Suggestions for further syntactic research on SASL are provided.
- Full Text:
- Date Issued: 2016
- Authors: De Barros, Courtney , Siebörger, Ian
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/385377 , vital:68013 , xlink:href=" https://hdl.handle.net/10520/EJC-4acb542a9"
- Description: As with other sign languages, South African Sign Language (SASL) expresses negation using both manual and non-manual features. In this case study, naturalistic data provided by two native signers of SASL are analysed to show the syntactic relationship between these two sets of features. Using a Principles and Parameters approach and Government and Binding Theory, we investigate the syntactic scope of negation in our SASL data. We observe that side-to-side headshake, as a non-manual feature, appears to be the chief clausal negator in SASL, with a clause-final manual negative particle, NOT, playing a secondary role. We describe the negative headshake as a featural affix which is base-generated in the head of NegP and triggers V-to- Neg raising. The negative particle NOT appears to be base-generated in the Specifier of NegP. Suggestions for further syntactic research on SASL are provided.
- Full Text:
- Date Issued: 2016
Sex and Loneliness: Banele Khoza’s ‘Temporary Feelings
- Authors: Lila, Philiswa
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/147149 , vital:38597 , https://artthrob.co.za/2016/07/28/sex-and-loneliness-banele-khozas-temporary-feelings/
- Description: Banele Khoza grapples with short sharp emotions in his solo exhibition at the Pretoria Art Museum. Entitled ‘Temporary Feelings,’ the exhibition is very personal, yet critically engaging with issues of relationships, communication, social media and sexuality. He brings his vulnerability into the gallery space: what it means to be lonely, even though surrounded by people.
- Full Text:
- Date Issued: 2016
- Authors: Lila, Philiswa
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/147149 , vital:38597 , https://artthrob.co.za/2016/07/28/sex-and-loneliness-banele-khozas-temporary-feelings/
- Description: Banele Khoza grapples with short sharp emotions in his solo exhibition at the Pretoria Art Museum. Entitled ‘Temporary Feelings,’ the exhibition is very personal, yet critically engaging with issues of relationships, communication, social media and sexuality. He brings his vulnerability into the gallery space: what it means to be lonely, even though surrounded by people.
- Full Text:
- Date Issued: 2016
Sha-1 and the strict avalanche criterion
- Motara, Yusuf, M, Irwin, Barry V W
- Authors: Motara, Yusuf, M , Irwin, Barry V W
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/429010 , vital:72553 , https://ieeexplore.ieee.org/abstract/document/7802926
- Description: The Strict Avalanche Criterion (SAC) is a measure of both confusion and diffusion, which are key properties of a cryptographic hash function. This work provides a working definition of the SAC, describes an experimental methodology that can be used to statistically evaluate whether a cryptographic hash meets the SAC, and uses this to investigate the degree to which compression function of the SHA-1 hash meets the SAC. The results (P 0.01) are heartening: SHA-1 closely tracks the SAC after the first 24 rounds, and demonstrates excellent properties of confusion and diffusion throughout.
- Full Text:
- Date Issued: 2016
- Authors: Motara, Yusuf, M , Irwin, Barry V W
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/429010 , vital:72553 , https://ieeexplore.ieee.org/abstract/document/7802926
- Description: The Strict Avalanche Criterion (SAC) is a measure of both confusion and diffusion, which are key properties of a cryptographic hash function. This work provides a working definition of the SAC, describes an experimental methodology that can be used to statistically evaluate whether a cryptographic hash meets the SAC, and uses this to investigate the degree to which compression function of the SHA-1 hash meets the SAC. The results (P 0.01) are heartening: SHA-1 closely tracks the SAC after the first 24 rounds, and demonstrates excellent properties of confusion and diffusion throughout.
- Full Text:
- Date Issued: 2016
South Africa in the Antarctic Circumnavigation Expedition: a multi-institutional and interdisciplinary scientific project
- Halo, Issufo, Dorrington, Rosemary A, Bornman, Thomas G, De Villiers, Stephanie, Fawcett, Sarah
- Authors: Halo, Issufo , Dorrington, Rosemary A , Bornman, Thomas G , De Villiers, Stephanie , Fawcett, Sarah
- Date: 2016
- Language: English
- Type: article
- Identifier: http://hdl.handle.net/10962/65428 , vital:28790 , https://doi.org/10.17159/sajs.2016/a0173
- Description: publisher version , The polar regions are more critically affected by climate change than any other region on our planet.1,2 On the Antarctic continent and in its surrounding oceans, the effects of climate change are likely to be dramatic,3 and include largescale catastrophic ice melt, loss of habitat and biodiversity, and global sea level rise. The ‘Southern Ocean’ refers to the region where Atlantic, Indian and Pacific Ocean waters come together to encircle Antarctica. These waters connect the different ocean basins by linking the shallow and deep limbs of the global ocean current system (‘overturning circulation’) and play a critical role in storing and distributing heat and carbon dioxide (CO2 ). The Southern Ocean thus regulates not only the climate of the Antarctic, but of the entire earth system.1,4 By extension, the capacity of the global ocean to ameliorate earth’s changing climate is strongly controlled by the Southern Ocean. Marine phytoplankton (microscopic plants inhabiting the sunlit upper ocean) convert CO2 (an inorganic form of carbon) dissolved in surface waters into organic carbon through photosynthesis. This organic carbon fuels upper trophic levels such as fish, mammals and birds, and a portion sinks into the deep ocean where it remains stored for hundreds to thousands of years. This mechanism, which lowers the atmospheric concentration of CO2 , is termed the ‘biological pump’.5 The efficiency of the global ocean’s biological pump is currently limited by the Southern Ocean, where the macronutrients (nitrate and phosphate) required for photosynthesis are never fully consumed in surface waters. In theory, increased consumption of these nutrients could drive higher organic carbon removal to the deep ocean, enhancing the oceanic uptake of atmospheric CO2 . Indeed, more complete consumption of Southern Ocean nutrients is a leading hypothesis for the decrease in atmospheric CO2 that characterised the ice ages.6 Despite the global importance of the Southern Ocean, knowledge of the controls on and interactions among the physical, chemical and biological processes operating in Antarctic ecosystems is limited, largely because of a scarcity of in-situ observational data, compounded by the challenge of integrating siloed scientific fields. Given predictions that diverse aspects of Southern Ocean physics and carbon biogeochemistry are likely to change in the coming decades, a transdisciplinary approach to studying Antarctic systems is critical.
- Full Text:
- Date Issued: 2016
- Authors: Halo, Issufo , Dorrington, Rosemary A , Bornman, Thomas G , De Villiers, Stephanie , Fawcett, Sarah
- Date: 2016
- Language: English
- Type: article
- Identifier: http://hdl.handle.net/10962/65428 , vital:28790 , https://doi.org/10.17159/sajs.2016/a0173
- Description: publisher version , The polar regions are more critically affected by climate change than any other region on our planet.1,2 On the Antarctic continent and in its surrounding oceans, the effects of climate change are likely to be dramatic,3 and include largescale catastrophic ice melt, loss of habitat and biodiversity, and global sea level rise. The ‘Southern Ocean’ refers to the region where Atlantic, Indian and Pacific Ocean waters come together to encircle Antarctica. These waters connect the different ocean basins by linking the shallow and deep limbs of the global ocean current system (‘overturning circulation’) and play a critical role in storing and distributing heat and carbon dioxide (CO2 ). The Southern Ocean thus regulates not only the climate of the Antarctic, but of the entire earth system.1,4 By extension, the capacity of the global ocean to ameliorate earth’s changing climate is strongly controlled by the Southern Ocean. Marine phytoplankton (microscopic plants inhabiting the sunlit upper ocean) convert CO2 (an inorganic form of carbon) dissolved in surface waters into organic carbon through photosynthesis. This organic carbon fuels upper trophic levels such as fish, mammals and birds, and a portion sinks into the deep ocean where it remains stored for hundreds to thousands of years. This mechanism, which lowers the atmospheric concentration of CO2 , is termed the ‘biological pump’.5 The efficiency of the global ocean’s biological pump is currently limited by the Southern Ocean, where the macronutrients (nitrate and phosphate) required for photosynthesis are never fully consumed in surface waters. In theory, increased consumption of these nutrients could drive higher organic carbon removal to the deep ocean, enhancing the oceanic uptake of atmospheric CO2 . Indeed, more complete consumption of Southern Ocean nutrients is a leading hypothesis for the decrease in atmospheric CO2 that characterised the ice ages.6 Despite the global importance of the Southern Ocean, knowledge of the controls on and interactions among the physical, chemical and biological processes operating in Antarctic ecosystems is limited, largely because of a scarcity of in-situ observational data, compounded by the challenge of integrating siloed scientific fields. Given predictions that diverse aspects of Southern Ocean physics and carbon biogeochemistry are likely to change in the coming decades, a transdisciplinary approach to studying Antarctic systems is critical.
- Full Text:
- Date Issued: 2016
Spectroscopic investigations and theoretical calculations of DABCO induced xanthene bridged self-assembled zinc (II) porphyrin dimer
- Xu, Li, Huang, Tingting, Liang, Xu, Mack, John, Harris, Jessica, Nyokong, Tebello, Li, Minzhi, Zhu, Weihua
- Authors: Xu, Li , Huang, Tingting , Liang, Xu , Mack, John , Harris, Jessica , Nyokong, Tebello , Li, Minzhi , Zhu, Weihua
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/240732 , vital:50866 , xlink:href="https://doi.org/10.1142/S1088424616500231"
- Description: An in-depth study of the electronic structure of a 1,4-diazabicyclo[2.2.2]octane (DABCO) induced molecular self-assembled xanthene-bridged and amide-bonded porphyrin dimer is reported. Density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations are used to identify trends in the optical spectroscopic properties. B3LYP geometry optimization predicts the formation of an almost perfectly eclipsed structure with respect to the two porphyrin rings with the analogous pyrrole nitrogens separated by 7.7–8.1 Å. The observed distinctive derivative-shaped band morphology of the pseudo-Faraday-A11 terms in the MCD spectra has been used to identify the main electronic Q and B-bands and to validate the TD-DFT calculations. The absence of a discernible splitting of the redox steps or a quenching of the fluorescence demonstrates that there is no significant exciton coupling between the two porphyrin rings.
- Full Text:
- Date Issued: 2016
- Authors: Xu, Li , Huang, Tingting , Liang, Xu , Mack, John , Harris, Jessica , Nyokong, Tebello , Li, Minzhi , Zhu, Weihua
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/240732 , vital:50866 , xlink:href="https://doi.org/10.1142/S1088424616500231"
- Description: An in-depth study of the electronic structure of a 1,4-diazabicyclo[2.2.2]octane (DABCO) induced molecular self-assembled xanthene-bridged and amide-bonded porphyrin dimer is reported. Density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations are used to identify trends in the optical spectroscopic properties. B3LYP geometry optimization predicts the formation of an almost perfectly eclipsed structure with respect to the two porphyrin rings with the analogous pyrrole nitrogens separated by 7.7–8.1 Å. The observed distinctive derivative-shaped band morphology of the pseudo-Faraday-A11 terms in the MCD spectra has been used to identify the main electronic Q and B-bands and to validate the TD-DFT calculations. The absence of a discernible splitting of the redox steps or a quenching of the fluorescence demonstrates that there is no significant exciton coupling between the two porphyrin rings.
- Full Text:
- Date Issued: 2016
Sport consumption patterns in the Eastern Cape: cricket spectators as sporting univores or omnivores
- Brock, Kelcey, Fraser, Gavin C G, Botha, Ferdi
- Authors: Brock, Kelcey , Fraser, Gavin C G , Botha, Ferdi
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/69362 , vital:29508 , https://doi.org/10.4102/jef.v9i3.64
- Description: Since its inception, consumption behaviour theory has developed to account for the important social aspects that underpin or at least to some extent explain consumer behaviour. Empirical studies on consumption behaviour of cultural activities, entertainment and sport have used Bourdieu’s (1984) omnivore/univore theory to investigate consumption of leisure activities. The aim of this study is to investigate whether South African cricket spectators are sporting omnivores or univores. The study was conducted among cricket spectators in the Eastern Cape at four limited overs cricket matches in the 2012/2013 cricket season. The results indicate that consumption behaviour of sport predominantly differs on the grounds of education and race. This suggests that there are aspects of social connotations underpinning sports consumption behaviour within South Africa.
- Full Text:
- Date Issued: 2016
Sport consumption patterns in the Eastern Cape: cricket spectators as sporting univores or omnivores
- Authors: Brock, Kelcey , Fraser, Gavin C G , Botha, Ferdi
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/69362 , vital:29508 , https://doi.org/10.4102/jef.v9i3.64
- Description: Since its inception, consumption behaviour theory has developed to account for the important social aspects that underpin or at least to some extent explain consumer behaviour. Empirical studies on consumption behaviour of cultural activities, entertainment and sport have used Bourdieu’s (1984) omnivore/univore theory to investigate consumption of leisure activities. The aim of this study is to investigate whether South African cricket spectators are sporting omnivores or univores. The study was conducted among cricket spectators in the Eastern Cape at four limited overs cricket matches in the 2012/2013 cricket season. The results indicate that consumption behaviour of sport predominantly differs on the grounds of education and race. This suggests that there are aspects of social connotations underpinning sports consumption behaviour within South Africa.
- Full Text:
- Date Issued: 2016
Spotlight on the Art of Darkness: The Kin Artstudio
- Authors: Tshilumba Mukendi, J S
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/146434 , vital:38525 , https://artafricamagazine.org/magazine-archive/?v=e4dd286dc7d7
- Description: Issue December 2016. What Really Matters.
- Full Text:
- Date Issued: 2016
- Authors: Tshilumba Mukendi, J S
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/146434 , vital:38525 , https://artafricamagazine.org/magazine-archive/?v=e4dd286dc7d7
- Description: Issue December 2016. What Really Matters.
- Full Text:
- Date Issued: 2016
Stromatolite microbial communities as a source of new bioactive secondary metabolites
- Flatt, P M, Damarjanan, C, Isamonger, E, Kalinski, Jarmo-Charles J, Dorrington, Rosemary A, McPhail, Kerry L
- Authors: Flatt, P M , Damarjanan, C , Isamonger, E , Kalinski, Jarmo-Charles J , Dorrington, Rosemary A , McPhail, Kerry L
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/65871 , vital:28851 , https://doi.org/10.1055/s-0036-1596638
- Description: publisher version , Stromatolites represent some of the earliest microbial communities on Earth. They are formed by accretion and precipitation of layered calcium carbonate structures that result from the metabolic activity of complex microbial communities and the geochemical conditions of their environment. Modern stromatolite communities include aerobic heterotrophs, sulphide-oxidizing bacteria, sulphate-reducing bacteria, fermentative bacteria and cyanobacteria. Phylogenetic analyses revealed the presence of new and known cyanobacterial taxa related to known producers of biologically active secondary metabolites in tufa stromatolites along the South African southeast coast [1]. Prompted us to investigate their potential for producing novel bioactive secondary metabolites. A series of three tide pools provided the opportunity to collect stromatolites along a vertical transect from pool A (highest elevation, low nitrogen input, fresh water), pool B (within high tide zone, brackish water) and pool C (within tidal zone). The microbial community in pool A is particularly distinct. Chemical extracts of stromatolites from different pools have been profiled by LC-MS/MS and the data subjected to molecular spectral networking using the GnPS platform [2] in order to establish the diversity and biological potential of the microbial metabolome that is being expressed within each of these microhabitats. Correlation of the phylogenetic and secondary metabolomic data is expected to guide the isolation of new natural products with biomedical relevance.
- Full Text: false
- Date Issued: 2016
- Authors: Flatt, P M , Damarjanan, C , Isamonger, E , Kalinski, Jarmo-Charles J , Dorrington, Rosemary A , McPhail, Kerry L
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/65871 , vital:28851 , https://doi.org/10.1055/s-0036-1596638
- Description: publisher version , Stromatolites represent some of the earliest microbial communities on Earth. They are formed by accretion and precipitation of layered calcium carbonate structures that result from the metabolic activity of complex microbial communities and the geochemical conditions of their environment. Modern stromatolite communities include aerobic heterotrophs, sulphide-oxidizing bacteria, sulphate-reducing bacteria, fermentative bacteria and cyanobacteria. Phylogenetic analyses revealed the presence of new and known cyanobacterial taxa related to known producers of biologically active secondary metabolites in tufa stromatolites along the South African southeast coast [1]. Prompted us to investigate their potential for producing novel bioactive secondary metabolites. A series of three tide pools provided the opportunity to collect stromatolites along a vertical transect from pool A (highest elevation, low nitrogen input, fresh water), pool B (within high tide zone, brackish water) and pool C (within tidal zone). The microbial community in pool A is particularly distinct. Chemical extracts of stromatolites from different pools have been profiled by LC-MS/MS and the data subjected to molecular spectral networking using the GnPS platform [2] in order to establish the diversity and biological potential of the microbial metabolome that is being expressed within each of these microhabitats. Correlation of the phylogenetic and secondary metabolomic data is expected to guide the isolation of new natural products with biomedical relevance.
- Full Text: false
- Date Issued: 2016
Structure based docking and molecular dynamic studies of plasmodial cysteine proteases against a South African natural compound and its analogs:
- Musyoka, Thommas M, Kanzi, Aquillah M, Lobb, Kevin A, Tastan Bishop, Özlem
- Authors: Musyoka, Thommas M , Kanzi, Aquillah M , Lobb, Kevin A , Tastan Bishop, Özlem
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148027 , vital:38703 , DOI: 10.1038/srep23690
- Description: Identification of potential drug targets as well as development of novel antimalarial chemotherapies with unique mode of actions due to drug resistance by Plasmodium parasites are inevitable. Falcipains (falcipain-2 and falcipain-3) of Plasmodium falciparum, which catalyse the haemoglobin degradation process, are validated drug targets. Previous attempts to develop peptide based drugs against these enzymes have been futile due to the poor pharmacological profiles and susceptibility to degradation by host enzymes. This study aimed to identify potential non-peptide inhibitors against falcipains and their homologs from other Plasmodium species.
- Full Text:
- Date Issued: 2016
- Authors: Musyoka, Thommas M , Kanzi, Aquillah M , Lobb, Kevin A , Tastan Bishop, Özlem
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148027 , vital:38703 , DOI: 10.1038/srep23690
- Description: Identification of potential drug targets as well as development of novel antimalarial chemotherapies with unique mode of actions due to drug resistance by Plasmodium parasites are inevitable. Falcipains (falcipain-2 and falcipain-3) of Plasmodium falciparum, which catalyse the haemoglobin degradation process, are validated drug targets. Previous attempts to develop peptide based drugs against these enzymes have been futile due to the poor pharmacological profiles and susceptibility to degradation by host enzymes. This study aimed to identify potential non-peptide inhibitors against falcipains and their homologs from other Plasmodium species.
- Full Text:
- Date Issued: 2016
Students’ reception of peer assessment of group-work contributions: problematics in terms of race and gender emerging from a South African case study
- Thondhlana, Gladman, Belluigi, Dina Z
- Authors: Thondhlana, Gladman , Belluigi, Dina Z
- Date: 2016
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/59784 , vital:27649 , https://doi.org/10.1080/02602938.2016.1235133
- Description: Participatory assessment is increasingly employed in higher education worldwide as a formative mechanism to support students’ active learning. But do students in an increasingly relationally diverse environment perceive that peer assessment of individuals’ contributions to group-work tasks enhances their learning? Recognising the impact of students’ conceptions on the quality of their learning, this study considers students’ perspectives of peer assessment of group-work contributions at a South African university. Questionnaires elicited students’ perspectives of and general attitudes towards assessment of and by their peers. A growing measure of discontent with the process of assessing peer contributions to group tasks emerged, including actual and perceived racial and gender stereotyping, and related rejection-sensitivity. These initial findings were checked against the students’ experiences in a report-and-respond process that enabled probing discussions of the interpretations. This paper examines and explores the implications of such identifications and receptions for learning engagement and group-work curriculum development in the context of a rapidly transforming higher education sector.
- Full Text: false
- Date Issued: 2016
- Authors: Thondhlana, Gladman , Belluigi, Dina Z
- Date: 2016
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/59784 , vital:27649 , https://doi.org/10.1080/02602938.2016.1235133
- Description: Participatory assessment is increasingly employed in higher education worldwide as a formative mechanism to support students’ active learning. But do students in an increasingly relationally diverse environment perceive that peer assessment of individuals’ contributions to group-work tasks enhances their learning? Recognising the impact of students’ conceptions on the quality of their learning, this study considers students’ perspectives of peer assessment of group-work contributions at a South African university. Questionnaires elicited students’ perspectives of and general attitudes towards assessment of and by their peers. A growing measure of discontent with the process of assessing peer contributions to group tasks emerged, including actual and perceived racial and gender stereotyping, and related rejection-sensitivity. These initial findings were checked against the students’ experiences in a report-and-respond process that enabled probing discussions of the interpretations. This paper examines and explores the implications of such identifications and receptions for learning engagement and group-work curriculum development in the context of a rapidly transforming higher education sector.
- Full Text: false
- Date Issued: 2016
Subcellular localisation of Theiler's murine encephalomyelitis virus (TMEV) capsid subunit VP1 vis-á-vis host protein Hsp90:
- Ross, Caroline J, Upfold, Nicole, Luke, Garry A, Tastan Bishop, Özlem, Knox, Caroline M
- Authors: Ross, Caroline J , Upfold, Nicole , Luke, Garry A , Tastan Bishop, Özlem , Knox, Caroline M
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148016 , vital:38702 , DOI: 10.1016/j.virusres.2016.06.003
- Description: The VP1 subunit of the picornavirus capsid is the major antigenic determinant and mediates host cell attachment and virus entry. To investigate the localisation of Theiler's murine encephalomyelitis virus (TMEV) VP1 during infection, a bioinformatics approach was used to predict a surface-exposed, linear epitope region of the protein for subsequent expression and purification. This region, comprising the N-terminal 112 amino acids of the protein, was then used for rabbit immunisation, and the resultant polyclonal antibodies were able to recognise full length VP1 in infected cell lysates by Western blot. Following optimisation, the antibodies were used to investigate the localisation of VP1 in relation to Hsp90 in infected cells by indirect immunofluorescence and confocal microscopy.
- Full Text:
- Date Issued: 2016
- Authors: Ross, Caroline J , Upfold, Nicole , Luke, Garry A , Tastan Bishop, Özlem , Knox, Caroline M
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148016 , vital:38702 , DOI: 10.1016/j.virusres.2016.06.003
- Description: The VP1 subunit of the picornavirus capsid is the major antigenic determinant and mediates host cell attachment and virus entry. To investigate the localisation of Theiler's murine encephalomyelitis virus (TMEV) VP1 during infection, a bioinformatics approach was used to predict a surface-exposed, linear epitope region of the protein for subsequent expression and purification. This region, comprising the N-terminal 112 amino acids of the protein, was then used for rabbit immunisation, and the resultant polyclonal antibodies were able to recognise full length VP1 in infected cell lysates by Western blot. Following optimisation, the antibodies were used to investigate the localisation of VP1 in relation to Hsp90 in infected cells by indirect immunofluorescence and confocal microscopy.
- Full Text:
- Date Issued: 2016