Plasmodium falciparum Hop: detailed analysis on complex formation with Hsp70 and Hsp90
- Hatherley, Rowan, Clitheroe, Crystal-Leigh, Faya, Ngonidzashe, Tastan Bishop, Özlem
- Authors: Hatherley, Rowan , Clitheroe, Crystal-Leigh , Faya, Ngonidzashe , Tastan Bishop, Özlem
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/163532 , vital:41046 , https://doi.org/10.1016/j.bbrc.2014.11.103
- Description: The heat shock organizing protein (Hop) is important in modulating the activity and co-interaction of two chaperones: heat shock protein 70 and 90 (Hsp70 and Hsp90). Recent research suggested that Plasmodium falciparum Hop (PfHop), PfHsp70 and PfHsp90 form a complex in the trophozoite infective stage. However, there has been little computational research on the malarial Hop protein in complex with other malarial Hsps.
- Full Text:
- Authors: Hatherley, Rowan , Clitheroe, Crystal-Leigh , Faya, Ngonidzashe , Tastan Bishop, Özlem
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/163532 , vital:41046 , https://doi.org/10.1016/j.bbrc.2014.11.103
- Description: The heat shock organizing protein (Hop) is important in modulating the activity and co-interaction of two chaperones: heat shock protein 70 and 90 (Hsp70 and Hsp90). Recent research suggested that Plasmodium falciparum Hop (PfHop), PfHsp70 and PfHsp90 form a complex in the trophozoite infective stage. However, there has been little computational research on the malarial Hop protein in complex with other malarial Hsps.
- Full Text:
Plasmodium falciparum Hop: detailed analysis on complex formation with Hsp70 and Hsp90
- Hatherley, Rowan, Clitheroe, Crystal-Leigh, Faya, Ngonidzashe, Tastan Bishop, Özlem
- Authors: Hatherley, Rowan , Clitheroe, Crystal-Leigh , Faya, Ngonidzashe , Tastan Bishop, Özlem
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125708 , vital:35810 , https://doi.10.1016/j.bbrc.2014.11.103
- Description: The heat shock organizing protein (Hop) is important in modulating the activity and co-interaction of two chaperones: heat shock protein 70 and 90 (Hsp70 and Hsp90). Recent research suggested that Plasmodium falciparum Hop (PfHop), PfHsp70 and PfHsp90 form a complex in the trophozoite infective stage. However, there has been little computational research on the malarial Hop protein in complex with other malarial Hsps. Using in silico characterization of the protein, this work showed that individual domains of Hop are evolving at different rates within the protein. Differences between human Hop (HsHop) and PfHop were identified by motif analysis. Homology modeling of PfHop and HsHop in complex with their own cytosolic Hsp90 and Hsp70 C-terminal peptide partners indicated excellent conservation of the Hop concave TPR sites bound to the C-terminal motifs of partner proteins. Further, we analyzed additional binding sites between Hop and Hsp90, and showed, for the first time, that they are distinctly less conserved between human and malaria parasite. These sites are located on the convex surface of Hop TPR2, and involved in interactions with the Hsp90 middle domain. Since the convex sites are less conserved than the concave sites, it makes their potential for malarial inhibitor design extremely attractive (as opposed to the concave sites which have been the focus of previous efforts).
- Full Text:
- Authors: Hatherley, Rowan , Clitheroe, Crystal-Leigh , Faya, Ngonidzashe , Tastan Bishop, Özlem
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125708 , vital:35810 , https://doi.10.1016/j.bbrc.2014.11.103
- Description: The heat shock organizing protein (Hop) is important in modulating the activity and co-interaction of two chaperones: heat shock protein 70 and 90 (Hsp70 and Hsp90). Recent research suggested that Plasmodium falciparum Hop (PfHop), PfHsp70 and PfHsp90 form a complex in the trophozoite infective stage. However, there has been little computational research on the malarial Hop protein in complex with other malarial Hsps. Using in silico characterization of the protein, this work showed that individual domains of Hop are evolving at different rates within the protein. Differences between human Hop (HsHop) and PfHop were identified by motif analysis. Homology modeling of PfHop and HsHop in complex with their own cytosolic Hsp90 and Hsp70 C-terminal peptide partners indicated excellent conservation of the Hop concave TPR sites bound to the C-terminal motifs of partner proteins. Further, we analyzed additional binding sites between Hop and Hsp90, and showed, for the first time, that they are distinctly less conserved between human and malaria parasite. These sites are located on the convex surface of Hop TPR2, and involved in interactions with the Hsp90 middle domain. Since the convex sites are less conserved than the concave sites, it makes their potential for malarial inhibitor design extremely attractive (as opposed to the concave sites which have been the focus of previous efforts).
- Full Text:
Politics and discourse in South Africa:
- Authors: Hunt, Sally
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/139249 , vital:37719 , ISBN 9789027206565 , https://benjamins.com/catalog/dapsac.65
- Description: The discourses of the post-apartheid South Africa embody symbols of change and promises of new lessons in history. This is the first volume that brings together analyses of a variety of discourses produced in South Africa through which we follow the evolution of transitional processes in the country’s political institutions and in the opinions of its populace. The book offers to the reader a visit to the Parliament, a peek into the internet forums, analyses of the country's official papers and speeches, and the media accounts. Through all these discourses we see the burning questions – "Who Are We Now?" and "Who Do We Want To Be?" – being repetitively examined and identities cross-formed while the country deals with new, post-apartheid challenges, as well as successes.
- Full Text:
- Authors: Hunt, Sally
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/139249 , vital:37719 , ISBN 9789027206565 , https://benjamins.com/catalog/dapsac.65
- Description: The discourses of the post-apartheid South Africa embody symbols of change and promises of new lessons in history. This is the first volume that brings together analyses of a variety of discourses produced in South Africa through which we follow the evolution of transitional processes in the country’s political institutions and in the opinions of its populace. The book offers to the reader a visit to the Parliament, a peek into the internet forums, analyses of the country's official papers and speeches, and the media accounts. Through all these discourses we see the burning questions – "Who Are We Now?" and "Who Do We Want To Be?" – being repetitively examined and identities cross-formed while the country deals with new, post-apartheid challenges, as well as successes.
- Full Text:
Polyurethane composite adsorbent using solid phase extraction method for preconcentration of metal ion from aqueous solution
- Olorundare, O F, Msagati, T A M, Okonkwo, J O, Krause, Rui W M, Mamba, Bhekie B
- Authors: Olorundare, O F , Msagati, T A M , Okonkwo, J O , Krause, Rui W M , Mamba, Bhekie B
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125386 , vital:35778 , https://doi.org/10.1007/s13762-014-0645-5
- Description: Polyurethane composite adsorbent polymeric material was prepared and investigated for selected solid-phase extraction for metal ions, prior to its determination by inductively coupled plasma optical emission spectrometry. The surface characterisation was done using Fourier transform infrared spectroscopy. The separation and preconcentration conditions of the analytes investigated includes influence of pH, sample loading flow rate, elution flow rate, type and concentration of eluents. The optimum pH for the highest efficient recoveries for all metal ions, which ranged from 70 to 85 %, is pH 7. The metal ions were quantitatively eluted with 5 mL of 2 mol/L HNO3. Common coexisting ions did not interfere with the separation. The percentage recovery of the metal ions ranged between 70 and 89 %, while the results for the limit of detection and limit of quantification ranged from 0.249 to 0.256 and 0.831 to 0.855, respectively. The experimental tests showed good preconcentration results of trace levels of metal ions using synthesised polyurethane polymer adsorbent composite.
- Full Text:
- Authors: Olorundare, O F , Msagati, T A M , Okonkwo, J O , Krause, Rui W M , Mamba, Bhekie B
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125386 , vital:35778 , https://doi.org/10.1007/s13762-014-0645-5
- Description: Polyurethane composite adsorbent polymeric material was prepared and investigated for selected solid-phase extraction for metal ions, prior to its determination by inductively coupled plasma optical emission spectrometry. The surface characterisation was done using Fourier transform infrared spectroscopy. The separation and preconcentration conditions of the analytes investigated includes influence of pH, sample loading flow rate, elution flow rate, type and concentration of eluents. The optimum pH for the highest efficient recoveries for all metal ions, which ranged from 70 to 85 %, is pH 7. The metal ions were quantitatively eluted with 5 mL of 2 mol/L HNO3. Common coexisting ions did not interfere with the separation. The percentage recovery of the metal ions ranged between 70 and 89 %, while the results for the limit of detection and limit of quantification ranged from 0.249 to 0.256 and 0.831 to 0.855, respectively. The experimental tests showed good preconcentration results of trace levels of metal ions using synthesised polyurethane polymer adsorbent composite.
- Full Text:
Population dynamics and biology of an invasive population of mosquitofish Gambusia affinis in a temperate estuarine lake system
- Sloterdijk, Hans, James, Nicola C, Smith, M Kyle S, Ekau, Werner, Weyl, Olaf L F
- Authors: Sloterdijk, Hans , James, Nicola C , Smith, M Kyle S , Ekau, Werner , Weyl, Olaf L F
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/443919 , vital:74171 , https://doi.org/10.1080/15627020.2015.1021169
- Description: This study investigates the distribution, relative abundance, population growth and biology of the alien invasive fish Gambusia affinis in the Wilderness Lakes System, a coastal lake system in the warm-temperate region of South Africa. Fish were collected throughout the system during four seasonal sampling trips in spring (October 2010), summer (February 2011), autumn (May 2011) and winter (July 2011). Gambusia affinis were widespread and abundant in all habitats from the freshwater reaches of the inflowing Touw River to the more saline lakes and estuary. Sex ratio was generally female dominated and mean length at maturity was 14.7 mm standard length (SL) for males and 20.3 mm SL for females. Reproduction was strongly seasonal with reproductively active fish sampled in spring and summer but not in autumn and winter. Relative abundance also varied seasonally, with populations following typical ‘boom and bust’ population dynamics. By winter, the population in all habitats was lower with mortality rates as high as 85%. The stronghold of the population appears to be in the channels between the lakes, where relative abundance was more consistent and winter mortality rates were lower. The survivors, which are primarily females, then reconstitute the population in the spring and summer months.
- Full Text:
- Authors: Sloterdijk, Hans , James, Nicola C , Smith, M Kyle S , Ekau, Werner , Weyl, Olaf L F
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/443919 , vital:74171 , https://doi.org/10.1080/15627020.2015.1021169
- Description: This study investigates the distribution, relative abundance, population growth and biology of the alien invasive fish Gambusia affinis in the Wilderness Lakes System, a coastal lake system in the warm-temperate region of South Africa. Fish were collected throughout the system during four seasonal sampling trips in spring (October 2010), summer (February 2011), autumn (May 2011) and winter (July 2011). Gambusia affinis were widespread and abundant in all habitats from the freshwater reaches of the inflowing Touw River to the more saline lakes and estuary. Sex ratio was generally female dominated and mean length at maturity was 14.7 mm standard length (SL) for males and 20.3 mm SL for females. Reproduction was strongly seasonal with reproductively active fish sampled in spring and summer but not in autumn and winter. Relative abundance also varied seasonally, with populations following typical ‘boom and bust’ population dynamics. By winter, the population in all habitats was lower with mortality rates as high as 85%. The stronghold of the population appears to be in the channels between the lakes, where relative abundance was more consistent and winter mortality rates were lower. The survivors, which are primarily females, then reconstitute the population in the spring and summer months.
- Full Text:
Precocious little monsters and the birth of puberty science: tracing early puberty as a health matter
- Pinto, Pedro, Macleod, Catriona I
- Authors: Pinto, Pedro , Macleod, Catriona I
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143626 , vital:38268 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Over the last two decades, early puberty has been increasingly portrayed in scientific and popular arenas as an alarming health issue. Changes in pubertal timing are frequently accorded a range of medical and moral dangers, suggesting individual degeneracy and social crisis. In our presentation – the first output of a Foucauldian genealogical investigation on pubertal knowledge in medical journals – we show that today’s problematisations of early puberty are rooted in the figure of the child monster, as produced in early nineteenth century medical discourse. Drawing on doctors’ clinical encounters with the pubescent body as represented in medical journals during that period, we argue that puberty, understood as a scientific construct, has been ‘praecox’ since the beginning. From this genealogical viewpoint, we explore the ways in which our present ‘pubertal complex’ talks to an old medical dilemma: the confusion of maturity and immaturity within the young body.
- Full Text:
- Authors: Pinto, Pedro , Macleod, Catriona I
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143626 , vital:38268 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Over the last two decades, early puberty has been increasingly portrayed in scientific and popular arenas as an alarming health issue. Changes in pubertal timing are frequently accorded a range of medical and moral dangers, suggesting individual degeneracy and social crisis. In our presentation – the first output of a Foucauldian genealogical investigation on pubertal knowledge in medical journals – we show that today’s problematisations of early puberty are rooted in the figure of the child monster, as produced in early nineteenth century medical discourse. Drawing on doctors’ clinical encounters with the pubescent body as represented in medical journals during that period, we argue that puberty, understood as a scientific construct, has been ‘praecox’ since the beginning. From this genealogical viewpoint, we explore the ways in which our present ‘pubertal complex’ talks to an old medical dilemma: the confusion of maturity and immaturity within the young body.
- Full Text:
Preface: proceedings of the 13th IASWS international conference
- Foster, Ian, Rowntree, Kate M, Ellery, William F N, Ogrinc, Nives, Oldham, Carolyn
- Authors: Foster, Ian , Rowntree, Kate M , Ellery, William F N , Ogrinc, Nives , Oldham, Carolyn
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144465 , vital:38348 , DOI 10.1007/s11368-015-1276-2
- Description: Rhodes University, Grahamstown, South Africa, was the venue for the 13th IASWS International Conference, held from 15 to 18 July, 2014. This international meeting built upon and expanded developing research in South Africa on sediment processes and followed on from a successful meeting organized by Rowntree and Foster on behalf of the Southern African Association of Geomorphologists (SAAG) held in Grahamstown in 2010 and the publication of a special issue of the journal Land Degradation and Development edited by Rowntree et al. (2012).
- Full Text:
- Authors: Foster, Ian , Rowntree, Kate M , Ellery, William F N , Ogrinc, Nives , Oldham, Carolyn
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144465 , vital:38348 , DOI 10.1007/s11368-015-1276-2
- Description: Rhodes University, Grahamstown, South Africa, was the venue for the 13th IASWS International Conference, held from 15 to 18 July, 2014. This international meeting built upon and expanded developing research in South Africa on sediment processes and followed on from a successful meeting organized by Rowntree and Foster on behalf of the Southern African Association of Geomorphologists (SAAG) held in Grahamstown in 2010 and the publication of a special issue of the journal Land Degradation and Development edited by Rowntree et al. (2012).
- Full Text:
Preliminary evidence for the organisation of a bacterial community by zooplanktivores at the top of an estuarine planktonic food web
- Wasserman, Ryan J, Matcher, Gwynneth F, Vink, Tim J F, Froneman, P William
- Authors: Wasserman, Ryan J , Matcher, Gwynneth F , Vink, Tim J F , Froneman, P William
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68237 , vital:29222 , https://doi.org/10.1007/s00248-014-0505-3
- Description: Publisher version , As part of a larger investigation, the effect of apex predation on estuarine bacterial community structure, through trophic cascading, was investigated using experimental in situ mesocosms. Through either the removal (filtration) or addition of specific size classes of planktonic groups, four different trophic scenarios were established using estuarine water and its associated plankton. One such treatment represented a “natural” scenario in which stable apex predatory pressure was qualified. Water samples were collected over time from each of the treatments for bacterial community evaluation. These samples were assessed through pyrosequencing of the variable regions 4 and 5 of the bacterial 16S rRNA gene and analysed at the species operational taxonomic unit (OTU) level using a community procedure. The blue-green group dominated the samples, followed by Proteobacteria and Bacteroidetes. Samples were the most similar among treatments at the commencement of the experiment. While the bacterial communities sampled within each treatment changed over time, the deviation from initial appeared to be linked to the treatment trophic scenarios. The least temporal deviation-from-initial in bacterial community was found within the stable apex predatory pressure treatment. These findings are consistent with trophic cascade theory, whereby predators mediate interactions at multiple lower trophic levels with consequent repercussions for diversity.
- Full Text: false
- Authors: Wasserman, Ryan J , Matcher, Gwynneth F , Vink, Tim J F , Froneman, P William
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68237 , vital:29222 , https://doi.org/10.1007/s00248-014-0505-3
- Description: Publisher version , As part of a larger investigation, the effect of apex predation on estuarine bacterial community structure, through trophic cascading, was investigated using experimental in situ mesocosms. Through either the removal (filtration) or addition of specific size classes of planktonic groups, four different trophic scenarios were established using estuarine water and its associated plankton. One such treatment represented a “natural” scenario in which stable apex predatory pressure was qualified. Water samples were collected over time from each of the treatments for bacterial community evaluation. These samples were assessed through pyrosequencing of the variable regions 4 and 5 of the bacterial 16S rRNA gene and analysed at the species operational taxonomic unit (OTU) level using a community procedure. The blue-green group dominated the samples, followed by Proteobacteria and Bacteroidetes. Samples were the most similar among treatments at the commencement of the experiment. While the bacterial communities sampled within each treatment changed over time, the deviation from initial appeared to be linked to the treatment trophic scenarios. The least temporal deviation-from-initial in bacterial community was found within the stable apex predatory pressure treatment. These findings are consistent with trophic cascade theory, whereby predators mediate interactions at multiple lower trophic levels with consequent repercussions for diversity.
- Full Text: false
Preparation and use of maize tassels’ activated carbon for the adsorption of phenolic compounds in environmental waste water samples
- Olorundare, O F, Msagati, T A M, Okonkwo, J O, Krause, Rui W M, Mamba, Bhekie B
- Authors: Olorundare, O F , Msagati, T A M , Okonkwo, J O , Krause, Rui W M , Mamba, Bhekie B
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125331 , vital:35773 , https://doi.org/10.1007/s11356-014-3742-6
- Description: The determination and remediation of three phenolic compounds bisphenol A (BPA), ortho-nitrophenol (o-NTP), parachlorophenol (PCP) in wastewater is reported. The analysis of these molecules in wastewater was done using gas chromatography (GC) × GC time-of-flight mass spectrometry while activated carbon derived from maize tassel was used as an adsorbent. During the experimental procedures, the effect of various parameters such as initial concentration, pH of sample solution, eluent volume, and sample volume on the removal efficiency with respect to the three phenolic compounds was studied. The results showed that maize tassel produced activated carbon (MTAC) cartridge packed solid-phase extraction (SPE) system was able to remove the phenolic compounds effectively (90.84–98.49 %, 80.75–97.11 %, and 78.27–97.08 % for BPA, o-NTP, and PCP, respectively) . The MTAC cartridge packed SPE sorbent performance was compared to commercially produced C18 SPE cartridges and found to be comparable. All the parameters investigated were found to have a notable influence on the adsorption efficiency of the phenolic compounds from wastewaters at different magnitudes.
- Full Text:
- Authors: Olorundare, O F , Msagati, T A M , Okonkwo, J O , Krause, Rui W M , Mamba, Bhekie B
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125331 , vital:35773 , https://doi.org/10.1007/s11356-014-3742-6
- Description: The determination and remediation of three phenolic compounds bisphenol A (BPA), ortho-nitrophenol (o-NTP), parachlorophenol (PCP) in wastewater is reported. The analysis of these molecules in wastewater was done using gas chromatography (GC) × GC time-of-flight mass spectrometry while activated carbon derived from maize tassel was used as an adsorbent. During the experimental procedures, the effect of various parameters such as initial concentration, pH of sample solution, eluent volume, and sample volume on the removal efficiency with respect to the three phenolic compounds was studied. The results showed that maize tassel produced activated carbon (MTAC) cartridge packed solid-phase extraction (SPE) system was able to remove the phenolic compounds effectively (90.84–98.49 %, 80.75–97.11 %, and 78.27–97.08 % for BPA, o-NTP, and PCP, respectively) . The MTAC cartridge packed SPE sorbent performance was compared to commercially produced C18 SPE cartridges and found to be comparable. All the parameters investigated were found to have a notable influence on the adsorption efficiency of the phenolic compounds from wastewaters at different magnitudes.
- Full Text:
Privilege, solidarity and social justice struggles in South Africa: a view from Grahamstown
- Authors: Matthews, Sally
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142270 , vital:38064 , DOI:10.1353/trn.2015.0016
- Description: The last decade has seen a significant increase in the number and prominence of social movements in South Africa. Many of these movements are supported by relatively privileged individuals who are not themselves victims of the injustices the movements oppose. In this paper, I draw out some of the possibilities and limitations of the role of privileged individuals in supporting social movements struggling for social justice, looking particularly at the role of students in supporting such movements. The paper is based on research on the relationship between one such movement, the Unemployed People’s Movement (UPM), and a student organisation, the Students for Social Justice (SSJ), both of which are based in Grahamstown, South Africa. While acknowledging that privileged supporters of such movements can play a constructive role in social justice struggles, I use the experiences of the UPM and SSJ to explore some of the tensions that are likely to emerge and that need to be addressed when the relatively privileged participate in popular struggles. In particular, I discuss the likely difficulties privileged supporters will experience in bridging social divides and in contributing meaningfully to the theorisation of popular struggles.
- Full Text:
- Authors: Matthews, Sally
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142270 , vital:38064 , DOI:10.1353/trn.2015.0016
- Description: The last decade has seen a significant increase in the number and prominence of social movements in South Africa. Many of these movements are supported by relatively privileged individuals who are not themselves victims of the injustices the movements oppose. In this paper, I draw out some of the possibilities and limitations of the role of privileged individuals in supporting social movements struggling for social justice, looking particularly at the role of students in supporting such movements. The paper is based on research on the relationship between one such movement, the Unemployed People’s Movement (UPM), and a student organisation, the Students for Social Justice (SSJ), both of which are based in Grahamstown, South Africa. While acknowledging that privileged supporters of such movements can play a constructive role in social justice struggles, I use the experiences of the UPM and SSJ to explore some of the tensions that are likely to emerge and that need to be addressed when the relatively privileged participate in popular struggles. In particular, I discuss the likely difficulties privileged supporters will experience in bridging social divides and in contributing meaningfully to the theorisation of popular struggles.
- Full Text:
Rapid bioassessment of the effects of repeated rotenone treatments on invertebrate assemblages in the Rondegat River, South Africa
- Bellingan, Terence A, Woodford, Darragh J, Gouws, J, Villet, Martin H, Weyl, Olaf L F
- Authors: Bellingan, Terence A , Woodford, Darragh J , Gouws, J , Villet, Martin H , Weyl, Olaf L F
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442279 , vital:73972 , https://doi.org/10.2989/16085914.2014.984651
- Description: The potential collateral effects of eradicating invasive fishes in streams necessitate the monitoring of invertebrate communities during treatment. In an environmental rehabilitation programme, non-native smallmouth bass were removed from the lower reaches of the Rondegat River, Western Cape, South Africa, in 2012 and again in 2013 using the piscicide rotenone. A monitoring programme tracked the ecological response of organisms to these activities using quantitative sampling of macroinvertebrates on stones and the ISO-certified SASS5 rapid bioassessment method for assessing macroinvertebrate community integrity. We recorded a significant decrease in macroinvertebrate densities from the stones-in-current biotope following both rotenone treatments. The average score per taxon (ASPT) declined after the first treatment, indicating a loss of taxa sensitive to diminished water quality, then recovered prior to the second treatment, and subsequently no decline was detected after the lower dose used in the 2013 treatment. The SASS values were too variable to reveal trends. The ASPTs indicated that the community may have been resistant to low dose and resilient to high dose, due to inter-treatment recovery following the 2012 treatment, suggesting that the invertebrate assemblage is resilient to the conservative use of rotenone for localised river rehabilitation when upstream sources of recruitment exist.
- Full Text:
- Authors: Bellingan, Terence A , Woodford, Darragh J , Gouws, J , Villet, Martin H , Weyl, Olaf L F
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442279 , vital:73972 , https://doi.org/10.2989/16085914.2014.984651
- Description: The potential collateral effects of eradicating invasive fishes in streams necessitate the monitoring of invertebrate communities during treatment. In an environmental rehabilitation programme, non-native smallmouth bass were removed from the lower reaches of the Rondegat River, Western Cape, South Africa, in 2012 and again in 2013 using the piscicide rotenone. A monitoring programme tracked the ecological response of organisms to these activities using quantitative sampling of macroinvertebrates on stones and the ISO-certified SASS5 rapid bioassessment method for assessing macroinvertebrate community integrity. We recorded a significant decrease in macroinvertebrate densities from the stones-in-current biotope following both rotenone treatments. The average score per taxon (ASPT) declined after the first treatment, indicating a loss of taxa sensitive to diminished water quality, then recovered prior to the second treatment, and subsequently no decline was detected after the lower dose used in the 2013 treatment. The SASS values were too variable to reveal trends. The ASPTs indicated that the community may have been resistant to low dose and resilient to high dose, due to inter-treatment recovery following the 2012 treatment, suggesting that the invertebrate assemblage is resilient to the conservative use of rotenone for localised river rehabilitation when upstream sources of recruitment exist.
- Full Text:
Reconsidering research ethics in ethnographic research: bearing witness to ‘irreparable harm’
- Barker, Kim, Macleod, Catriona I
- Authors: Barker, Kim , Macleod, Catriona I
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143805 , vital:38284 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Research with persons who have experienced trauma requires careful consideration. In preparing the ethics protocol for an ethnographic study of an anti-rape protest, we thought carefully about how the first author would manage ethical decisions in accordance with the University ethics code. However, this process did not prepare us for the dynamic and reciprocal positioning the first author encountered in the field. Nor was she prepared for her sense of the ethical duty of response when entrusted with the narratives of women who had suffered ‘irredeemable harm’. Drawing on the philosophy of Emmanuel Levinas, and examples from the research, we show how ethical decision-making in ethnographic research is always relational and dialogical; extending beyond our direct interactions with participants to the ways in which we approach our ‘data’. We argue that ethics cannot be reduced to a cognitive-rational process and propose ways to acknowledge and draw on the ‘affective’ and ‘transcendent’ in our ethical decision-making.
- Full Text:
- Authors: Barker, Kim , Macleod, Catriona I
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143805 , vital:38284 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Research with persons who have experienced trauma requires careful consideration. In preparing the ethics protocol for an ethnographic study of an anti-rape protest, we thought carefully about how the first author would manage ethical decisions in accordance with the University ethics code. However, this process did not prepare us for the dynamic and reciprocal positioning the first author encountered in the field. Nor was she prepared for her sense of the ethical duty of response when entrusted with the narratives of women who had suffered ‘irredeemable harm’. Drawing on the philosophy of Emmanuel Levinas, and examples from the research, we show how ethical decision-making in ethnographic research is always relational and dialogical; extending beyond our direct interactions with participants to the ways in which we approach our ‘data’. We argue that ethics cannot be reduced to a cognitive-rational process and propose ways to acknowledge and draw on the ‘affective’ and ‘transcendent’ in our ethical decision-making.
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Reflecting on patient-centred care in pharmacy through an illness narrative:
- Authors: Dowse, Roslind
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/156687 , vital:40038 , https://0-doi.org.wam.seals.ac.za/10.1007/s11096-015-0104-5
- Description: Patient-centred care (PCC) is rapidly adopting a central position in discussions on the quality of healthcare, with patient-centredness deemed essential to transforming the healthcare system. PCC speaks to the quality of patient-provider relationships and has been defined as an approach to providing care that is respectful of and responsive to individual patient preferences, needs, and values, while ensuring that patient values guide all clinical decisions. However its place within pharmacy practice is unclear and is as yet undefined, particularly in relation to pharmaceutical care. Through my personal illness narrative, I briefly explore the visibility and evidence of PCC in the pharmacy literature as well as from personal experience of pharmacy care, and find it lacking. I conclude that an integrated, seamless understanding of PCC and the use of shared language within the health professions is essential in successful teamwork with both the patient and with other health professions.
- Full Text:
- Authors: Dowse, Roslind
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/156687 , vital:40038 , https://0-doi.org.wam.seals.ac.za/10.1007/s11096-015-0104-5
- Description: Patient-centred care (PCC) is rapidly adopting a central position in discussions on the quality of healthcare, with patient-centredness deemed essential to transforming the healthcare system. PCC speaks to the quality of patient-provider relationships and has been defined as an approach to providing care that is respectful of and responsive to individual patient preferences, needs, and values, while ensuring that patient values guide all clinical decisions. However its place within pharmacy practice is unclear and is as yet undefined, particularly in relation to pharmaceutical care. Through my personal illness narrative, I briefly explore the visibility and evidence of PCC in the pharmacy literature as well as from personal experience of pharmacy care, and find it lacking. I conclude that an integrated, seamless understanding of PCC and the use of shared language within the health professions is essential in successful teamwork with both the patient and with other health professions.
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Reinventing the oral word and returning it to the community via technauriture
- Kaschula, Russell H, Dlutu, Bongiwe
- Authors: Kaschula, Russell H , Dlutu, Bongiwe
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/67052 , vital:29024 , https://books.google.co.za/books?id=6IpwCgAAQBAJ
- Description: publisher version , From Introduction: The aim of this article is to situate the importance of orality in rural communities within the paradigm of Technauriture, which is defined below. The chapter will also explore/describe the process of orality supported through community meetings, oral histories, and story-telling, and how it interacts with the recording process facilitated through modern technology, as well as the return of the oral material via technology. These objectives will be pursued as part of empirical data collected at Tshani, an area falling within the in Mankosi tribal authority in the Eastern Cape Province of South Africa. Mankosi’s twelve villages are inhabited by amaXhosa people and is situated in the Ngqeleni district in the former Transkei region. Mankosi is about 76 kilometres away from Mthatha, the major city in that area. These villages are under the Nyandeni Local Municipality and are regarded as very disadvantaged. There is poor sanitation, gravel roads, and few local people have access to electricity. Most of the villagers depend on government support grants. These include Child Support Grants, Old Age Grants, Disability Grants, and Foster-Care Grants. Furthermore, most of the community is involved in small-scale agriculture for survival. People often move, temporarily, from their village to towns in order to gain employment, including domestic work and working on the mines in the mineral rich Gauteng Province. They send cash remittances home to their families. The primary technological devices that people in the village are familiar with would be radio and the cell phone. Even television sets are relatively uncommon.
- Full Text: false
- Authors: Kaschula, Russell H , Dlutu, Bongiwe
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/67052 , vital:29024 , https://books.google.co.za/books?id=6IpwCgAAQBAJ
- Description: publisher version , From Introduction: The aim of this article is to situate the importance of orality in rural communities within the paradigm of Technauriture, which is defined below. The chapter will also explore/describe the process of orality supported through community meetings, oral histories, and story-telling, and how it interacts with the recording process facilitated through modern technology, as well as the return of the oral material via technology. These objectives will be pursued as part of empirical data collected at Tshani, an area falling within the in Mankosi tribal authority in the Eastern Cape Province of South Africa. Mankosi’s twelve villages are inhabited by amaXhosa people and is situated in the Ngqeleni district in the former Transkei region. Mankosi is about 76 kilometres away from Mthatha, the major city in that area. These villages are under the Nyandeni Local Municipality and are regarded as very disadvantaged. There is poor sanitation, gravel roads, and few local people have access to electricity. Most of the villagers depend on government support grants. These include Child Support Grants, Old Age Grants, Disability Grants, and Foster-Care Grants. Furthermore, most of the community is involved in small-scale agriculture for survival. People often move, temporarily, from their village to towns in order to gain employment, including domestic work and working on the mines in the mineral rich Gauteng Province. They send cash remittances home to their families. The primary technological devices that people in the village are familiar with would be radio and the cell phone. Even television sets are relatively uncommon.
- Full Text: false
Representations of gender and agency in the Harry Potter series:
- Authors: Hunt, Sally
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/139269 , vital:37721 , ISBN 978-1-137-43173-8 , https://doi.org/10.1057/9781137431738_13
- Description: Gender is an all-pervasive and extremely influential construct in the lives of individuals (Taylor, 2003). In children’s literature, we find a reflection of the attitudes towards gender prevalent in a given society at a particular time (Peterson and Lach, 1990). Therefore the study of how gender is represented in children’s literature can make a useful contribution to our understanding of how choices in language use support particular discourses, ‘broad constitutive systems of meaning’ (Sunderland, 2004: 6) or ‘ways of seeing the world’ (op cit: 28). These representations in turn perpetuate prevailing gendered power relations in that society, as research into children’s literature has shown (Thompson and Sealey, 2007). Corpus Linguistics offers a degree of objectivity and efficiency not possible in manual ideological analysis, as well as a set of tools particularly useful for the lexical analysis of considerable quantities of text. In this chapter, I report on my analysis of gendered discourses in the Harry Potter series by J. K. Rowling, focussing on patterns around grammatical agency in the books.
- Full Text:
- Authors: Hunt, Sally
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/139269 , vital:37721 , ISBN 978-1-137-43173-8 , https://doi.org/10.1057/9781137431738_13
- Description: Gender is an all-pervasive and extremely influential construct in the lives of individuals (Taylor, 2003). In children’s literature, we find a reflection of the attitudes towards gender prevalent in a given society at a particular time (Peterson and Lach, 1990). Therefore the study of how gender is represented in children’s literature can make a useful contribution to our understanding of how choices in language use support particular discourses, ‘broad constitutive systems of meaning’ (Sunderland, 2004: 6) or ‘ways of seeing the world’ (op cit: 28). These representations in turn perpetuate prevailing gendered power relations in that society, as research into children’s literature has shown (Thompson and Sealey, 2007). Corpus Linguistics offers a degree of objectivity and efficiency not possible in manual ideological analysis, as well as a set of tools particularly useful for the lexical analysis of considerable quantities of text. In this chapter, I report on my analysis of gendered discourses in the Harry Potter series by J. K. Rowling, focussing on patterns around grammatical agency in the books.
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Resemiotising concerns from constituencies in the South African parliament
- Siebörger, Ian, Adendorff, Ralph D
- Authors: Siebörger, Ian , Adendorff, Ralph D
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/385362 , vital:68011 , xlink:href="https://doi.org/10.2989/16073614.2015.1061892"
- Description: Members of Parliament (MPs) in South Africa represent different constituencies across the country. In this article, we report on how MPs resemiotise concerns from their constituencies in spoken discourse in a parliamentary committee, and on the effectiveness with which this informa- tion is in turn resemiotised into a written committee report. Both resemiotisations form part of a genre chain which we investigated while conducting a linguistic ethnography of the communica- tion difficulties which occur in parliament's committee process. We use a multi-stranded theoretical foundation, including tools from Systemic Functional Linguistics, Interactional Sociolinguistics and Legitimation Code Theory to analyse MPs’ ability to communicate concerns from their constituen- cies in parliamentary discourse. We conclude that the success of MPs’ resemiotisations of these concerns depends on their ability to rescale them as relevant on a national level, and on their ability to negotiate the power relations at play in parliament.
- Full Text:
- Authors: Siebörger, Ian , Adendorff, Ralph D
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/385362 , vital:68011 , xlink:href="https://doi.org/10.2989/16073614.2015.1061892"
- Description: Members of Parliament (MPs) in South Africa represent different constituencies across the country. In this article, we report on how MPs resemiotise concerns from their constituencies in spoken discourse in a parliamentary committee, and on the effectiveness with which this informa- tion is in turn resemiotised into a written committee report. Both resemiotisations form part of a genre chain which we investigated while conducting a linguistic ethnography of the communica- tion difficulties which occur in parliament's committee process. We use a multi-stranded theoretical foundation, including tools from Systemic Functional Linguistics, Interactional Sociolinguistics and Legitimation Code Theory to analyse MPs’ ability to communicate concerns from their constituen- cies in parliamentary discourse. We conclude that the success of MPs’ resemiotisations of these concerns depends on their ability to rescale them as relevant on a national level, and on their ability to negotiate the power relations at play in parliament.
- Full Text:
Restricted physical activity research in older women: does this represent additional risk to health?
- Viljoen, Janet E, Christie, Candice J
- Authors: Viljoen, Janet E , Christie, Candice J
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143682 , vital:38273 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Post-menopausal women are at great risk of developing cardiovascular disease due to the loss of endogenous estrogen at menopause. This risk can be mediated via medication, but this route is costly and carries the risk of side effects which can reduce quality of life experience. The benefit of a lifestyle management approach to such risk, particularly via physical activity, is the emotional wellbeing that accompanies the physical health improvement. Research has not focused on women post-menses in this regard, and the existing science has preferred investigations of low intensity, aerobic type exercise. Our research engaged women, post-menses, in a high frequency, high intensity resistance training programme for 12 weeks in a supervised setting. Apart from clinical health benefits, the participants reported feeling empowered by the intervention. This paper will argue that the predominating research focus has tended to reduce choices for women, and thus restrict independence, individuality and self-confidence.
- Full Text:
Restricted physical activity research in older women: does this represent additional risk to health?
- Authors: Viljoen, Janet E , Christie, Candice J
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143682 , vital:38273 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Post-menopausal women are at great risk of developing cardiovascular disease due to the loss of endogenous estrogen at menopause. This risk can be mediated via medication, but this route is costly and carries the risk of side effects which can reduce quality of life experience. The benefit of a lifestyle management approach to such risk, particularly via physical activity, is the emotional wellbeing that accompanies the physical health improvement. Research has not focused on women post-menses in this regard, and the existing science has preferred investigations of low intensity, aerobic type exercise. Our research engaged women, post-menses, in a high frequency, high intensity resistance training programme for 12 weeks in a supervised setting. Apart from clinical health benefits, the participants reported feeling empowered by the intervention. This paper will argue that the predominating research focus has tended to reduce choices for women, and thus restrict independence, individuality and self-confidence.
- Full Text:
Review of the projected impacts of climate change on coastal fishes in southern Africa
- Potts, Warren M, Götz, A, James, Nicola Caroline
- Authors: Potts, Warren M , Götz, A , James, Nicola Caroline
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125899 , vital:35830 , https://doi.10.1007/s11160-015-9399-5
- Description: The coastal zone represents one of the most economically and ecologically important ecosystems on the planet, none more so than in southern Africa. This manuscript examines the potential impacts of climate change on the coastal fishes in southern Africa and provides some of the first information for the Southern Hemisphere, outside of Australasia. It begins by describing the coastal zone in terms of its physical characteristics, climate, fish biodiversity and fisheries. The region is divided into seven biogeographical zones based on previous descriptions and interpretations by the authors. A global review of the impacts of climate change on coastal zones is then applied to make qualitative predictions on the likely impacts of climate change on migratory, resident, estuarine-dependent and catadromous fishes in each of these biogeographical zones. In many respects the southern African region represents a microcosm of climate change variability and of coastal habitats. Based on the broad range of climate change impacts and life history styles of coastal fishes, the predicted impacts on fishes will be diverse. If anything, this review reveals our lack of fundamental knowledge in this field, in particular in southern Africa. Several research priorities, including the need for process-based fundamental research programs are highlighted.
- Full Text:
- Authors: Potts, Warren M , Götz, A , James, Nicola Caroline
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125899 , vital:35830 , https://doi.10.1007/s11160-015-9399-5
- Description: The coastal zone represents one of the most economically and ecologically important ecosystems on the planet, none more so than in southern Africa. This manuscript examines the potential impacts of climate change on the coastal fishes in southern Africa and provides some of the first information for the Southern Hemisphere, outside of Australasia. It begins by describing the coastal zone in terms of its physical characteristics, climate, fish biodiversity and fisheries. The region is divided into seven biogeographical zones based on previous descriptions and interpretations by the authors. A global review of the impacts of climate change on coastal zones is then applied to make qualitative predictions on the likely impacts of climate change on migratory, resident, estuarine-dependent and catadromous fishes in each of these biogeographical zones. In many respects the southern African region represents a microcosm of climate change variability and of coastal habitats. Based on the broad range of climate change impacts and life history styles of coastal fishes, the predicted impacts on fishes will be diverse. If anything, this review reveals our lack of fundamental knowledge in this field, in particular in southern Africa. Several research priorities, including the need for process-based fundamental research programs are highlighted.
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Rewarding tax compliance: taxpayers’ attitudes and beliefs
- Bornman, Marina, Stack, Elizabeth M
- Authors: Bornman, Marina , Stack, Elizabeth M
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/61039 , vital:27931
- Description: In a society the tax climate is determined by the interaction between taxpayers and tax authorities. In a ‘service and client’ climate, taxpayers do not expect authorities to automatically suspect them of being tax evaders. Evidence suggests that recognising good tax behaviour with strategies of rewards has a positive effect on voluntary tax compliance. Principles derived from the cognitive evaluation theory predict that when feelings of competence are affirmed and this is accompanied by a sense of autonomy it can enhance the intrinsic motivation for an action. The present research surveyed the attitudes and beliefs of taxpayers involved in small business on being rewarded for tax compliance. Results were corroborated with the principles of the cognitive evaluation theory and it was found that that the principles of the theory are applicable to rewarding tax compliance behaviour.
- Full Text:
- Authors: Bornman, Marina , Stack, Elizabeth M
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/61039 , vital:27931
- Description: In a society the tax climate is determined by the interaction between taxpayers and tax authorities. In a ‘service and client’ climate, taxpayers do not expect authorities to automatically suspect them of being tax evaders. Evidence suggests that recognising good tax behaviour with strategies of rewards has a positive effect on voluntary tax compliance. Principles derived from the cognitive evaluation theory predict that when feelings of competence are affirmed and this is accompanied by a sense of autonomy it can enhance the intrinsic motivation for an action. The present research surveyed the attitudes and beliefs of taxpayers involved in small business on being rewarded for tax compliance. Results were corroborated with the principles of the cognitive evaluation theory and it was found that that the principles of the theory are applicable to rewarding tax compliance behaviour.
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Rise and fall of apartheid: photography and the bureaucracy of everyday life
- Authors: Simbao, Ruth Kerkham, 1969-
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125910 , vital:35831 , https://doi.10.1080/02533952.2014.998052
- Description: The exhibition catalogue Rise and Fall of Apartheid is a valuable collection of photographic images that create, according to Enwezor, “a critical visualization and interrogation of […] [apartheid’s] normative symbols, signs and representation” (18). The catalogue focuses on African subjects as “agents of their own emancipation” (18), and contextualises South Africa’s anticipation of the end of apartheid within broader global changes in the late 1980s. Essays by Okwui Enwezor, Michael Godby, Achille Mbembe, Darren Newbury, Colin Richards, Patricia Hayes, Andries Walter Olifant, Rory Bester and Khwezi Gule are included in the catalogue, and are interspersed between photographic images that are grouped in chronological clusters: 1948–1959; 1960–1969; 1970–1979; 1980–1989; and 1990–1995.
- Full Text:
- Authors: Simbao, Ruth Kerkham, 1969-
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125910 , vital:35831 , https://doi.10.1080/02533952.2014.998052
- Description: The exhibition catalogue Rise and Fall of Apartheid is a valuable collection of photographic images that create, according to Enwezor, “a critical visualization and interrogation of […] [apartheid’s] normative symbols, signs and representation” (18). The catalogue focuses on African subjects as “agents of their own emancipation” (18), and contextualises South Africa’s anticipation of the end of apartheid within broader global changes in the late 1980s. Essays by Okwui Enwezor, Michael Godby, Achille Mbembe, Darren Newbury, Colin Richards, Patricia Hayes, Andries Walter Olifant, Rory Bester and Khwezi Gule are included in the catalogue, and are interspersed between photographic images that are grouped in chronological clusters: 1948–1959; 1960–1969; 1970–1979; 1980–1989; and 1990–1995.
- Full Text: