Thermophilic lignin degrading enzymes from actinomycetes for biotechnological applications
- Authors: Mhlanga, Chido Yvonne Lois
- Date: 2002 , 2013-05-16
- Subjects: Actinomycetales -- Biotechnology , Lignin
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4085 , http://hdl.handle.net/10962/d1007628 , Actinomycetales -- Biotechnology , Lignin
- Description: Phenolic residues which accumulate in the environment as a result of agro-industrial practices has resulted in the need to find and use Eco-Friendly techniques, rather than the traditional methods of burning or burying this kind of waste. Bioremediation and bioconversion are attractive alternatives using whole cell or enzyme-based systems. The aims of this project were to isolate and uses thermophilic Actinomycetes, which produce thermo-tolerant oxidoreductase enzymes, which can be used to bioconvert a model industrial phenolic waste commonly genersated in the wine-making industry of South Africa. Current research in bioconversion and bioremediation focuses on mesophilic microbes in that their enzymes can catalyse reactions at higher temperatures without affecting its activity and lower contamination levels. Three novel Actinomycete isolates were isolated (RU-A0l , RU-A03 and RU-A06) from a compost site and characterized using a combination of conventional identification techniques and 16S rDNA methodology to identity the three isolates. All three isolates belong to the Streptomyces clade. In addition, five known Actinomycetes were selected from an internation culture collection and also screened for oxidoreductase activity in comparision to the three novel isolates. Although the five isolates were selected based on their ability to produce oxidoreductase enzymes, unexpectedly, no activity was detected. Screening assays for peroxidase, polyphenol oxidase and laccase on RU-AO 1, RU-A03 and RU-A06, showed that all three isolated produced peroxidases and peroxidases but no laccase. Substrate specificity studies revealed that the most suitable substrates to determine peroxidase and polyphenol oxidase activity on these isolates were catechol for polyphenol oxidase, 2,4-dichlorophenol for peroxidases and veratryl alcohol for lignin peroxidases. Previous studies have indicated that peroxidases and polyphenol oxidases are produced in Actinomycetes during the primary stage of growth. This was the case with RU-AOI , RU-A03 and RU-A06. Growth rates were higher that other Actinomycetes, with maxImum biomass being reached at 36 hours for the isolates RU-AOI and RU-A06 and 48 hours for isolate RUA03. pH studies showed that the three isolates were adaptable and could grow over a broad pH range. Catabolism studies of phenolic model compounds showed that the three isolates were capable of catabolizing the model phenolic compounds within a period of 24 hours. Further studies were carried out to determine the effect of these microbes and their enzymes in whole cell and enzyme-based systems on a model phenolic waste, graoe waste consisting of compressed grape skins, pips and stalks. Whole cell studies showed that the isolates were capable of bioconverting the waste at a maximum concentration of 30% grape waste (vol:vol). Peroxidase and polyphenol oxidase activity increased indicating induction of these enzymes in the presence of phenolic compounds, with a maximum increase of up to 15.9 fold increase in extracellular lignin peroxidase activity in RU-AO1. HPLC and phenolic determination assays indicated that bioconversion of the phenolic grape waste had occurred in the presence of the three isolates. Attempts were made to isolate and identify a peroxidase or phenol oxidase gene from one the isolates. As bacteria, Actinomycetes are amendable to gene manipulation making them suitable candidates for methods such as site directed evolution in comparison to fungi. Two clones were selected for sequencing based on positive activity results when assayed for peroxidase activity. However the resultant sequences did not identify a functional gene sequence. Southern Blotting was then carried out to determine the nature of the peroxidase gene. Previous studies have been focused on the catalase-peroxidase gene (CalC gene) found Actinomycetes and other bacteria. A probe was developed from the CalC gene. No hybridization occurred with any of the enzyme restricted DNA from the three isolates. The implications of these results are that the peroxidase genets in the three isolates are in fact lignin peroxidase in nature. This project has the potential in the bioconversion of phenolic wastes and is the first description of the use of thermophilic Actinomycetes in the bioconversion of an industrial phenolic waste.
- Full Text:
- Authors: Mhlanga, Chido Yvonne Lois
- Date: 2002 , 2013-05-16
- Subjects: Actinomycetales -- Biotechnology , Lignin
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4085 , http://hdl.handle.net/10962/d1007628 , Actinomycetales -- Biotechnology , Lignin
- Description: Phenolic residues which accumulate in the environment as a result of agro-industrial practices has resulted in the need to find and use Eco-Friendly techniques, rather than the traditional methods of burning or burying this kind of waste. Bioremediation and bioconversion are attractive alternatives using whole cell or enzyme-based systems. The aims of this project were to isolate and uses thermophilic Actinomycetes, which produce thermo-tolerant oxidoreductase enzymes, which can be used to bioconvert a model industrial phenolic waste commonly genersated in the wine-making industry of South Africa. Current research in bioconversion and bioremediation focuses on mesophilic microbes in that their enzymes can catalyse reactions at higher temperatures without affecting its activity and lower contamination levels. Three novel Actinomycete isolates were isolated (RU-A0l , RU-A03 and RU-A06) from a compost site and characterized using a combination of conventional identification techniques and 16S rDNA methodology to identity the three isolates. All three isolates belong to the Streptomyces clade. In addition, five known Actinomycetes were selected from an internation culture collection and also screened for oxidoreductase activity in comparision to the three novel isolates. Although the five isolates were selected based on their ability to produce oxidoreductase enzymes, unexpectedly, no activity was detected. Screening assays for peroxidase, polyphenol oxidase and laccase on RU-AO 1, RU-A03 and RU-A06, showed that all three isolated produced peroxidases and peroxidases but no laccase. Substrate specificity studies revealed that the most suitable substrates to determine peroxidase and polyphenol oxidase activity on these isolates were catechol for polyphenol oxidase, 2,4-dichlorophenol for peroxidases and veratryl alcohol for lignin peroxidases. Previous studies have indicated that peroxidases and polyphenol oxidases are produced in Actinomycetes during the primary stage of growth. This was the case with RU-AOI , RU-A03 and RU-A06. Growth rates were higher that other Actinomycetes, with maxImum biomass being reached at 36 hours for the isolates RU-AOI and RU-A06 and 48 hours for isolate RUA03. pH studies showed that the three isolates were adaptable and could grow over a broad pH range. Catabolism studies of phenolic model compounds showed that the three isolates were capable of catabolizing the model phenolic compounds within a period of 24 hours. Further studies were carried out to determine the effect of these microbes and their enzymes in whole cell and enzyme-based systems on a model phenolic waste, graoe waste consisting of compressed grape skins, pips and stalks. Whole cell studies showed that the isolates were capable of bioconverting the waste at a maximum concentration of 30% grape waste (vol:vol). Peroxidase and polyphenol oxidase activity increased indicating induction of these enzymes in the presence of phenolic compounds, with a maximum increase of up to 15.9 fold increase in extracellular lignin peroxidase activity in RU-AO1. HPLC and phenolic determination assays indicated that bioconversion of the phenolic grape waste had occurred in the presence of the three isolates. Attempts were made to isolate and identify a peroxidase or phenol oxidase gene from one the isolates. As bacteria, Actinomycetes are amendable to gene manipulation making them suitable candidates for methods such as site directed evolution in comparison to fungi. Two clones were selected for sequencing based on positive activity results when assayed for peroxidase activity. However the resultant sequences did not identify a functional gene sequence. Southern Blotting was then carried out to determine the nature of the peroxidase gene. Previous studies have been focused on the catalase-peroxidase gene (CalC gene) found Actinomycetes and other bacteria. A probe was developed from the CalC gene. No hybridization occurred with any of the enzyme restricted DNA from the three isolates. The implications of these results are that the peroxidase genets in the three isolates are in fact lignin peroxidase in nature. This project has the potential in the bioconversion of phenolic wastes and is the first description of the use of thermophilic Actinomycetes in the bioconversion of an industrial phenolic waste.
- Full Text:
Towards a broader socio-ecological education : a case study of school based curricular reform
- Authors: Cimma, Gary Martin
- Date: 2002
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:20973 , http://hdl.handle.net/10962/5763
- Description: This work documents and analyses an action research project that the researcher undertook in conjunction with colleagues at an independent Catholic high school in Johannesburg. The main objective was to try to expand the existing curriculum in operation at the time of starting the research to one, which was more socio-ecologically sensitive. One of the key references during the action research was the recently drafted ‘Vision Statement’ document, which detailed the educational ideals of the school. Some of the key findings that emerged as the research proceeded, were that the levels of socio-ecological sensitivity displayed by the learners were influenced by the backgrounds from which they came, and that these background environments were often not comfortable with the changes taking place in the educational and greater South African environments. The Participatory Action Research mode of research which was used proved to be problematic in that only a handful of the educators actually got involved in participating in the project. The failure of many of educators to get involved was, in the opinion of the researcher, due to their lack of understanding how important the development of social and ecological sensitivity are to the necessary change capacity required in present day South African society. The need to be involved in the ongoing development of curriculum as required by Curriculum 2005 as a form of Outcomes Based Education was also problematic as some educators were not familiar with their role in this regard. The limited time-span of the research was also a limiting factor as the development of a socio-ecologically sensitive curriculum required changes in some fundamental attitudes and approaches to education and the environment.
- Full Text:
- Authors: Cimma, Gary Martin
- Date: 2002
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:20973 , http://hdl.handle.net/10962/5763
- Description: This work documents and analyses an action research project that the researcher undertook in conjunction with colleagues at an independent Catholic high school in Johannesburg. The main objective was to try to expand the existing curriculum in operation at the time of starting the research to one, which was more socio-ecologically sensitive. One of the key references during the action research was the recently drafted ‘Vision Statement’ document, which detailed the educational ideals of the school. Some of the key findings that emerged as the research proceeded, were that the levels of socio-ecological sensitivity displayed by the learners were influenced by the backgrounds from which they came, and that these background environments were often not comfortable with the changes taking place in the educational and greater South African environments. The Participatory Action Research mode of research which was used proved to be problematic in that only a handful of the educators actually got involved in participating in the project. The failure of many of educators to get involved was, in the opinion of the researcher, due to their lack of understanding how important the development of social and ecological sensitivity are to the necessary change capacity required in present day South African society. The need to be involved in the ongoing development of curriculum as required by Curriculum 2005 as a form of Outcomes Based Education was also problematic as some educators were not familiar with their role in this regard. The limited time-span of the research was also a limiting factor as the development of a socio-ecologically sensitive curriculum required changes in some fundamental attitudes and approaches to education and the environment.
- Full Text:
Towards the performer-creator in contemporary mime, with specific reference to the physical theatre of Andrew Buckland, 1988-2000
- Authors: Murray, Robert Ian
- Date: 2002
- Subjects: Buckland, Andrew , Mime , Mimes -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2158 , http://hdl.handle.net/10962/d1007720
- Description: Part one of this thesis investigates the conceptualisation of the performer-creator and its relevance in late twentieth century physical performance by examining some of the theoretical, but mainly artistic, traditions that suggest a movement towards this. Chapter one tackles the question of definition, linking mime within a wider physical theatre phenomenon. Chapter two looks at the importance of mime training for contemporary performance. This requires a focus on selected theatre practitioners who have significantly advanced the development of mime training and performance during the twentieth century. Chapter three examines the issue of silence in mime and questions a potential liberation of the word and language in contemporary performance and consequently how this affects the role of the performer, particularly in South Africa. Chapter four investigates the figure of the theatrical clown and how this late twentieth century development is playing an increasingly important role in contemporary performance. Chapter five then wraps up part one by pulling the preceding chapters together and thereby providing a working conceptualisation of the performer-creator, locating it within an overall appreciation of contemporary mime. Part two then tests the notion of the performer-creator by focussing on a case study through the investigation of Andrew Buckland to develop this idea. Andrew Buckland, and his work under the ethos of Mouthpeace from 1988-2000, provides a clear and unique example of this movement in contemporary South African performance. Trained as an actor and in classical illusion-based mime technique, as well as many dance forms such as ballet, contemporary and jazz, he has virtually single-handedly created and shaped a particular performance trend that is in line with contemporary international trends but retains a distinctive South African flavour. There is no space to attempt a definitive or exhaustive examination of his works, and nor is the intent to do so; rather, the aim is to draw from his work their essences that reveal his development as an artist: as both a creator and a performer.
- Full Text:
- Authors: Murray, Robert Ian
- Date: 2002
- Subjects: Buckland, Andrew , Mime , Mimes -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2158 , http://hdl.handle.net/10962/d1007720
- Description: Part one of this thesis investigates the conceptualisation of the performer-creator and its relevance in late twentieth century physical performance by examining some of the theoretical, but mainly artistic, traditions that suggest a movement towards this. Chapter one tackles the question of definition, linking mime within a wider physical theatre phenomenon. Chapter two looks at the importance of mime training for contemporary performance. This requires a focus on selected theatre practitioners who have significantly advanced the development of mime training and performance during the twentieth century. Chapter three examines the issue of silence in mime and questions a potential liberation of the word and language in contemporary performance and consequently how this affects the role of the performer, particularly in South Africa. Chapter four investigates the figure of the theatrical clown and how this late twentieth century development is playing an increasingly important role in contemporary performance. Chapter five then wraps up part one by pulling the preceding chapters together and thereby providing a working conceptualisation of the performer-creator, locating it within an overall appreciation of contemporary mime. Part two then tests the notion of the performer-creator by focussing on a case study through the investigation of Andrew Buckland to develop this idea. Andrew Buckland, and his work under the ethos of Mouthpeace from 1988-2000, provides a clear and unique example of this movement in contemporary South African performance. Trained as an actor and in classical illusion-based mime technique, as well as many dance forms such as ballet, contemporary and jazz, he has virtually single-handedly created and shaped a particular performance trend that is in line with contemporary international trends but retains a distinctive South African flavour. There is no space to attempt a definitive or exhaustive examination of his works, and nor is the intent to do so; rather, the aim is to draw from his work their essences that reveal his development as an artist: as both a creator and a performer.
- Full Text:
Traditional leaders in post-1996 South Africa, with particular reference to the Eastern Cape
- De Sas Kropiwnicki, Zosa Olenka
- Authors: De Sas Kropiwnicki, Zosa Olenka
- Date: 2002
- Subjects: Tribal government -- South Africa -- Eastern Cape , Political leadership -- South Africa -- Eastern Cape , South Africa -- Politics and government
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2857 , http://hdl.handle.net/10962/d1007479 , Tribal government -- South Africa -- Eastern Cape , Political leadership -- South Africa -- Eastern Cape , South Africa -- Politics and government
- Description: The failure of democracy in Africa can be partially attributed to the Eurocentric assumptions that belie Western recommendations for Africa. This thesis focuses on the failure of the modernisation school to account for the resiliency of tradition in the modern African state, which is described by Sklar (1991) as amounting to a form of 'mixed government', combining the traditional with the modern to create a uniquely African form of governance. This notion of a 'mixed government' is addressed from the vantage point of traditional leaders in the Eastern Cape. It maps the vacillating relationship between the chiefs, the people and the government through colonialism, Apartheid and democratisation. It concludes that although the Eastern Cape provincial government has subordinated the chiefs, this does not signify a victory for modernity over tradition because the chiefs are not a spent force. History has shown that when the government fails to act in the interests of the people, they seek an alternative authority namely, the chiefs. The ANC government's centralising tendencies have negative implications for democracy and consequently for the people. This opens up space for the chiefs to assert themselves provided they play an active role in furthering democracy, development and modernisation in the interests of the people. Hence, although ' mixed' government in the post-1996 South Africa is currently on the ANC's terms, traditional leaders may someday play a vital role in the modern democratic state.
- Full Text:
- Authors: De Sas Kropiwnicki, Zosa Olenka
- Date: 2002
- Subjects: Tribal government -- South Africa -- Eastern Cape , Political leadership -- South Africa -- Eastern Cape , South Africa -- Politics and government
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2857 , http://hdl.handle.net/10962/d1007479 , Tribal government -- South Africa -- Eastern Cape , Political leadership -- South Africa -- Eastern Cape , South Africa -- Politics and government
- Description: The failure of democracy in Africa can be partially attributed to the Eurocentric assumptions that belie Western recommendations for Africa. This thesis focuses on the failure of the modernisation school to account for the resiliency of tradition in the modern African state, which is described by Sklar (1991) as amounting to a form of 'mixed government', combining the traditional with the modern to create a uniquely African form of governance. This notion of a 'mixed government' is addressed from the vantage point of traditional leaders in the Eastern Cape. It maps the vacillating relationship between the chiefs, the people and the government through colonialism, Apartheid and democratisation. It concludes that although the Eastern Cape provincial government has subordinated the chiefs, this does not signify a victory for modernity over tradition because the chiefs are not a spent force. History has shown that when the government fails to act in the interests of the people, they seek an alternative authority namely, the chiefs. The ANC government's centralising tendencies have negative implications for democracy and consequently for the people. This opens up space for the chiefs to assert themselves provided they play an active role in furthering democracy, development and modernisation in the interests of the people. Hence, although ' mixed' government in the post-1996 South Africa is currently on the ANC's terms, traditional leaders may someday play a vital role in the modern democratic state.
- Full Text:
Use of indigenous and indigenised medicines to enhance personal well-being: a South African case study
- Cocks, Michelle L, Moller, Valerie
- Authors: Cocks, Michelle L , Moller, Valerie
- Date: 2002
- Language: English
- Type: Article
- Identifier: vital:7106 , http://hdl.handle.net/10962/d1010679
- Description: An estimated 27 million South Africans use indigenous medicines (Mander, 1997, Medicinal plant marketing and strategies for sustaining the plant supply in the Bushbuckridge area and Mpumalanga Province. Institute for Natural Resources, University of Natal, Pietermaritzburg, South Africa). Although herbal remedies are freely available in amayeza stores, or Xhosa chemists, for self-medication, little is known about the motivations of consumers. According to African belief systems, good health is holistic and extends to the person's social environment. The paper makes a distinction between traditional medicines which are used to enhance personal well-being generally and for cultural purposes, on the one hand, and medicines used to treat physical conditions only, on the other. Drawing on an eight-month study of Xhosa chemists in Eastern Cape Province, South Africa, in 1996, the paper identifies 90 medicines in stock which are used to enhance personal well-being. Just under one-third of all purchases were of medicines to enhance well-being. Remedies particularly popular included medicines believed to ward off evil spirits and bring good luck. The protection of infants with medicines which repel evil spirits is a common practice. Consumer behaviours indicate that the range of medicines available is increased by indigenisation of manufactured traditional medicines and cross-cultural borrowing. Case studies confirm that self- and infant medication with indigenous remedies augmented by indigenised medicines plays an important role in primary health care by allaying the fears and anxieties of everyday life within the Xhosa belief system, thereby promoting personal well-being.
- Full Text:
- Authors: Cocks, Michelle L , Moller, Valerie
- Date: 2002
- Language: English
- Type: Article
- Identifier: vital:7106 , http://hdl.handle.net/10962/d1010679
- Description: An estimated 27 million South Africans use indigenous medicines (Mander, 1997, Medicinal plant marketing and strategies for sustaining the plant supply in the Bushbuckridge area and Mpumalanga Province. Institute for Natural Resources, University of Natal, Pietermaritzburg, South Africa). Although herbal remedies are freely available in amayeza stores, or Xhosa chemists, for self-medication, little is known about the motivations of consumers. According to African belief systems, good health is holistic and extends to the person's social environment. The paper makes a distinction between traditional medicines which are used to enhance personal well-being generally and for cultural purposes, on the one hand, and medicines used to treat physical conditions only, on the other. Drawing on an eight-month study of Xhosa chemists in Eastern Cape Province, South Africa, in 1996, the paper identifies 90 medicines in stock which are used to enhance personal well-being. Just under one-third of all purchases were of medicines to enhance well-being. Remedies particularly popular included medicines believed to ward off evil spirits and bring good luck. The protection of infants with medicines which repel evil spirits is a common practice. Consumer behaviours indicate that the range of medicines available is increased by indigenisation of manufactured traditional medicines and cross-cultural borrowing. Case studies confirm that self- and infant medication with indigenous remedies augmented by indigenised medicines plays an important role in primary health care by allaying the fears and anxieties of everyday life within the Xhosa belief system, thereby promoting personal well-being.
- Full Text:
Use patterns and value of savanna resources in three rural villages in South Africa
- Shackleton, Sheona E, Shackleton, Charlie M, Netshiluvhi, T R, Geach, B S, Ballance, A, Fairbanks, D H K
- Authors: Shackleton, Sheona E , Shackleton, Charlie M , Netshiluvhi, T R , Geach, B S , Ballance, A , Fairbanks, D H K
- Date: 2002
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/182384 , vital:43825 , xlink:href="https://doi.org/10.1663/0013-0001(2002)056[0130:UPAVOS]2.0.CO;2"
- Description: Rural communities in South Africa harvest a diversity of wild resources from communal woodlands for home consumption and sale. The contribution these resources make to the rural economy has been little recognized, and few studies have attempted to place a monetary value on this use. This paper describes three case studies which aimed to determine the value of savanna resources for the livelihoods of rural households. Use patterns and values of resources in three villages of differing socioeconomic status were determined using household interviews, PRA techniques and key informant interviews. Questions were designed to establish the types of products used, frequency of use, quantities used, seasonality of use, longevity of durable resources, local prices, and the extent of trade. All households were procuring at least some woodland resources, with the most frequently used being fuel wood, wood for implements, edible herbs and fruits, grass for brushes, and insects. Patterns of resource use varied across villages. The most “rural” village used the greatest diversity of resources and had the highest number of users for most resources. Gross value of resources consumed per household per year ranged from R28I9 to R7238. Total value was highest in the less obviously resource dependent village, primarily the result of higher local prices due to greater extraction costs and a larger market for traded goods. Values are comparable to those contributed by other land-based livelihood activities such as subsistence cultivation and livestock production.
- Full Text:
- Authors: Shackleton, Sheona E , Shackleton, Charlie M , Netshiluvhi, T R , Geach, B S , Ballance, A , Fairbanks, D H K
- Date: 2002
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/182384 , vital:43825 , xlink:href="https://doi.org/10.1663/0013-0001(2002)056[0130:UPAVOS]2.0.CO;2"
- Description: Rural communities in South Africa harvest a diversity of wild resources from communal woodlands for home consumption and sale. The contribution these resources make to the rural economy has been little recognized, and few studies have attempted to place a monetary value on this use. This paper describes three case studies which aimed to determine the value of savanna resources for the livelihoods of rural households. Use patterns and values of resources in three villages of differing socioeconomic status were determined using household interviews, PRA techniques and key informant interviews. Questions were designed to establish the types of products used, frequency of use, quantities used, seasonality of use, longevity of durable resources, local prices, and the extent of trade. All households were procuring at least some woodland resources, with the most frequently used being fuel wood, wood for implements, edible herbs and fruits, grass for brushes, and insects. Patterns of resource use varied across villages. The most “rural” village used the greatest diversity of resources and had the highest number of users for most resources. Gross value of resources consumed per household per year ranged from R28I9 to R7238. Total value was highest in the less obviously resource dependent village, primarily the result of higher local prices due to greater extraction costs and a larger market for traded goods. Values are comparable to those contributed by other land-based livelihood activities such as subsistence cultivation and livestock production.
- Full Text:
Valuation of intellectual capital in South African companies: a comparative study of three valuation methods
- Authors: Maree, Kevin W
- Date: 2002
- Subjects: Accounting
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:877 , http://hdl.handle.net/10962/d1001631
- Description: This study discusses three valuation methods for intellectual capital and considers two of these (Tobin’s “q” and CIV) as suitable valuation methods.
- Full Text:
- Authors: Maree, Kevin W
- Date: 2002
- Subjects: Accounting
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:877 , http://hdl.handle.net/10962/d1001631
- Description: This study discusses three valuation methods for intellectual capital and considers two of these (Tobin’s “q” and CIV) as suitable valuation methods.
- Full Text:
Valuing the arts: pitfalls in economic impact studies of arts festivals
- Snowball, Jeanette D, Antrobus, Geoffrey G
- Authors: Snowball, Jeanette D , Antrobus, Geoffrey G
- Date: 2002
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143145 , vital:38205 , https://doi.org/10.1111/j.1813-6982.2002.tb00067.x
- Description: Economic impact studies have been used to measure the value of a variety of public and mixed goods, such as arts festivals, sports facilities and educational institutions, partly to motivate for public funds. The attraction of this sort of study rests largely on the fact that it produces a quantifiable monetary measure of the value of a project as opposed to a less easily valued qualitative study. “Public officials, boosters and the media accept the ‘quantifiable’ which appears to represent reality in order to justify a desired project” (Johnson and Sack 1996:370).
- Full Text:
- Authors: Snowball, Jeanette D , Antrobus, Geoffrey G
- Date: 2002
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143145 , vital:38205 , https://doi.org/10.1111/j.1813-6982.2002.tb00067.x
- Description: Economic impact studies have been used to measure the value of a variety of public and mixed goods, such as arts festivals, sports facilities and educational institutions, partly to motivate for public funds. The attraction of this sort of study rests largely on the fact that it produces a quantifiable monetary measure of the value of a project as opposed to a less easily valued qualitative study. “Public officials, boosters and the media accept the ‘quantifiable’ which appears to represent reality in order to justify a desired project” (Johnson and Sack 1996:370).
- Full Text:
Voltammetric characterization of the self-assembled monolayer (SAM) of octabutylthiophthalocyaninatoiron (II)
- Ozoemena,Kenneth, Nyokong, Tebello
- Authors: Ozoemena,Kenneth , Nyokong, Tebello
- Date: 2002
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/290419 , vital:56748 , xlink:href="https://doi.org/10.1016/S0013-4686(02)00362-6"
- Description: The fabrication of a self-assembled monolayer (SAM) of 2,3,9,10,16,17,23,24-octa (butylthio)-phthalocyaninatoiron(II) [FePc(SBu)8] on gold electrode is described. The integrity of the SAM, with respect to its ability to block certain Faradaic processes, is interrogated using cyclic voltammetric experiments in aqueous solutions. The experiments show that this SAM provide an excellent blocking capability to the Faradaic processes emanating from gold surface oxidation, underpotential deposition (UPD) of copper and redox chemistry of Fe(NH4)(SO4)2 in HClO4. It is revealed by cyclic voltammetry that an ill-defined reversible couple of the SAM of FePc(SBu)8 can be greatly improved by a simple repetitive cycling of the modified electrode in a DMF solution containing TBAP within a short space of time (ca. 2 min). This ‘activation’ process provides good information concerning the surface coverage and orientation of the monolayer. The reversible redox wave shows a potential shift of about −57 mV per pH in the pH range of 2–9. A preliminary investigation indicates that FePc(SBu)8-SAM modified gold electrode shows electrocatalytic activity toward the oxidation of L-cysteine in acidic medium. The monolayer is stable and easily reproducible. However, due to its susceptibility to destruction via oxidative and reductive desorptions, its potential application as an electrochemical sensor would be much better in acidic and neutral than alkaline environments.
- Full Text:
- Authors: Ozoemena,Kenneth , Nyokong, Tebello
- Date: 2002
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/290419 , vital:56748 , xlink:href="https://doi.org/10.1016/S0013-4686(02)00362-6"
- Description: The fabrication of a self-assembled monolayer (SAM) of 2,3,9,10,16,17,23,24-octa (butylthio)-phthalocyaninatoiron(II) [FePc(SBu)8] on gold electrode is described. The integrity of the SAM, with respect to its ability to block certain Faradaic processes, is interrogated using cyclic voltammetric experiments in aqueous solutions. The experiments show that this SAM provide an excellent blocking capability to the Faradaic processes emanating from gold surface oxidation, underpotential deposition (UPD) of copper and redox chemistry of Fe(NH4)(SO4)2 in HClO4. It is revealed by cyclic voltammetry that an ill-defined reversible couple of the SAM of FePc(SBu)8 can be greatly improved by a simple repetitive cycling of the modified electrode in a DMF solution containing TBAP within a short space of time (ca. 2 min). This ‘activation’ process provides good information concerning the surface coverage and orientation of the monolayer. The reversible redox wave shows a potential shift of about −57 mV per pH in the pH range of 2–9. A preliminary investigation indicates that FePc(SBu)8-SAM modified gold electrode shows electrocatalytic activity toward the oxidation of L-cysteine in acidic medium. The monolayer is stable and easily reproducible. However, due to its susceptibility to destruction via oxidative and reductive desorptions, its potential application as an electrochemical sensor would be much better in acidic and neutral than alkaline environments.
- Full Text:
Voltammetric detection of vitamin B1 at carbon paste electrodes and its determination in tablets
- Oni, Joshua, Westbroek, Philippe, Nyokong, Tebello
- Authors: Oni, Joshua , Westbroek, Philippe , Nyokong, Tebello
- Date: 2002
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/289692 , vital:56663 , xlink:href="https://doi.org/10.1002/1521-4109(200209)14"
- Description: The voltammetric behavior of vitamin B1 in alkaline medium at carbon paste electrode (CPE) is discussed. A method based on cyclic voltammetry for the determination of vitamin B1 at carbon paste electrode modified with manganese phthalocyanine (MnPc-CPE) is presented. In pH 10 buffer, the modified electrode showed catalytic activity towards the oxidation of vitamin B1 by considerably increasing the oxidation current of one of the observed waves compared to the oxidation current obtained at an unmodified electrode. The modified electrode was stable towards the determination of vitamin B1 compared to the unmodified electrode that is completely poisoned after a few scans. A linear relationship between the oxidation current and the concentration of vitamin B1 in solution was observed. The detection limit for vitamin B1 at the modified electrode was of the order of 10−5 mol dm−3. Commercial vitamin B1 tablets were analyzed using the modified electrodes.
- Full Text:
- Authors: Oni, Joshua , Westbroek, Philippe , Nyokong, Tebello
- Date: 2002
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/289692 , vital:56663 , xlink:href="https://doi.org/10.1002/1521-4109(200209)14"
- Description: The voltammetric behavior of vitamin B1 in alkaline medium at carbon paste electrode (CPE) is discussed. A method based on cyclic voltammetry for the determination of vitamin B1 at carbon paste electrode modified with manganese phthalocyanine (MnPc-CPE) is presented. In pH 10 buffer, the modified electrode showed catalytic activity towards the oxidation of vitamin B1 by considerably increasing the oxidation current of one of the observed waves compared to the oxidation current obtained at an unmodified electrode. The modified electrode was stable towards the determination of vitamin B1 compared to the unmodified electrode that is completely poisoned after a few scans. A linear relationship between the oxidation current and the concentration of vitamin B1 in solution was observed. The detection limit for vitamin B1 at the modified electrode was of the order of 10−5 mol dm−3. Commercial vitamin B1 tablets were analyzed using the modified electrodes.
- Full Text:
Voltammetric detection of vitamin B1 at carbon paste electrodes and its determination in tablets
- Oni, Joshua, Westbroek, Philippe, Nyokong, Tebello
- Authors: Oni, Joshua , Westbroek, Philippe , Nyokong, Tebello
- Date: 2002
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/289678 , vital:56662 , xlink:href="https://doi.org/10.1002/1521-4109(200209)14"
- Description: The voltammetric behavior of vitamin B1 in alkaline medium at carbon paste electrode (CPE) is discussed. A method based on cyclic voltammetry for the determination of vitamin B1 at carbon paste electrode modified with manganese phthalocyanine (MnPc-CPE) is presented. In pH 10 buffer, the modified electrode showed catalytic activity towards the oxidation of vitamin B1 by considerably increasing the oxidation current of one of the observed waves compared to the oxidation current obtained at an unmodified electrode. The modified electrode was stable towards the determination of vitamin B1 compared to the unmodified electrode that is completely poisoned after a few scans. A linear relationship between the oxidation current and the concentration of vitamin B1 in solution was observed. The detection limit for vitamin B1 at the modified electrode was of the order of 10−5 mol dm−3. Commercial vitamin B1 tablets were analyzed using the modified electrodes.
- Full Text:
- Authors: Oni, Joshua , Westbroek, Philippe , Nyokong, Tebello
- Date: 2002
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/289678 , vital:56662 , xlink:href="https://doi.org/10.1002/1521-4109(200209)14"
- Description: The voltammetric behavior of vitamin B1 in alkaline medium at carbon paste electrode (CPE) is discussed. A method based on cyclic voltammetry for the determination of vitamin B1 at carbon paste electrode modified with manganese phthalocyanine (MnPc-CPE) is presented. In pH 10 buffer, the modified electrode showed catalytic activity towards the oxidation of vitamin B1 by considerably increasing the oxidation current of one of the observed waves compared to the oxidation current obtained at an unmodified electrode. The modified electrode was stable towards the determination of vitamin B1 compared to the unmodified electrode that is completely poisoned after a few scans. A linear relationship between the oxidation current and the concentration of vitamin B1 in solution was observed. The detection limit for vitamin B1 at the modified electrode was of the order of 10−5 mol dm−3. Commercial vitamin B1 tablets were analyzed using the modified electrodes.
- Full Text:
Voluntary associations as schools for democracy? : a case study of the Sibanye Development Project
- Authors: Siwahla, Lindiwe Lillian
- Date: 2002
- Subjects: Sibanye Development Project , Non-governmental organizations , Political participation , Non-governmental organizations--South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2842 , http://hdl.handle.net/10962/d1004778 , Sibanye Development Project , Non-governmental organizations , Political participation , Non-governmental organizations--South Africa
- Description: This is an empirical study of a voluntary association with a view to interrogate the theories of civil society and participation and their practice. These theories came to dominate debate on African politics and democratisation following disappointment with structural approaches to development and democracy. Disenchantment with the state whose role was emphasised by the structural approach led analysts and technocrats to turn their attention to human agency; hence the salience of the idea of popular participation in the public domain, and preoccupation with the idea of strengthening civil society. This trend gained momentum after the 1989 fall of the Berlin Wall and demise of the communist block, and was accompanied by anti-statist sentiments on a global scale. Civil society organisations are seen as schools for democracy and agents of democratic consolidation, and are accordingly expected to perform two major tasks, namely instilling and disseminating a democratic political culture in and among participants and society at large, and promoting good governance. The aim therefore is to take advantage of the supposed intrinsic and utilitarian benefits of participation. As evident in a number of policy documents and legislation, the incumbent South African government embraces the idea of participatory democracy. However, not all analysts share this confidence in the capacity of civil society to perform these tasks. For some analysts public participation does not always have positive intrinsic benefits. Public participation may instead lead to a corrupted political culture deriving from the participants' attempts to survive in a public sphere characterised by manipulation and subtle political control, and it is civil society organisations lacking in organisational strength that are particularly vulnerable. The study revealed that unity between practice and theories of participation and civil society is a complex matter fraught with a number of ambiguities and contradictions. It revealed that though participation in the voluntary association in question does have educative benefits, those benefits do not extend to all the participants. In addition, the quality of that education is contingent upon a number of factors, some internal, others external. The internal and external factors reinforce one another. The internal factors pertain to the organisational dynamics of the voluntary association itself, and the external factors to the nature of the relationship between the voluntary association concerned and public authorities and other civil society organisations. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Siwahla, Lindiwe Lillian
- Date: 2002
- Subjects: Sibanye Development Project , Non-governmental organizations , Political participation , Non-governmental organizations--South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2842 , http://hdl.handle.net/10962/d1004778 , Sibanye Development Project , Non-governmental organizations , Political participation , Non-governmental organizations--South Africa
- Description: This is an empirical study of a voluntary association with a view to interrogate the theories of civil society and participation and their practice. These theories came to dominate debate on African politics and democratisation following disappointment with structural approaches to development and democracy. Disenchantment with the state whose role was emphasised by the structural approach led analysts and technocrats to turn their attention to human agency; hence the salience of the idea of popular participation in the public domain, and preoccupation with the idea of strengthening civil society. This trend gained momentum after the 1989 fall of the Berlin Wall and demise of the communist block, and was accompanied by anti-statist sentiments on a global scale. Civil society organisations are seen as schools for democracy and agents of democratic consolidation, and are accordingly expected to perform two major tasks, namely instilling and disseminating a democratic political culture in and among participants and society at large, and promoting good governance. The aim therefore is to take advantage of the supposed intrinsic and utilitarian benefits of participation. As evident in a number of policy documents and legislation, the incumbent South African government embraces the idea of participatory democracy. However, not all analysts share this confidence in the capacity of civil society to perform these tasks. For some analysts public participation does not always have positive intrinsic benefits. Public participation may instead lead to a corrupted political culture deriving from the participants' attempts to survive in a public sphere characterised by manipulation and subtle political control, and it is civil society organisations lacking in organisational strength that are particularly vulnerable. The study revealed that unity between practice and theories of participation and civil society is a complex matter fraught with a number of ambiguities and contradictions. It revealed that though participation in the voluntary association in question does have educative benefits, those benefits do not extend to all the participants. In addition, the quality of that education is contingent upon a number of factors, some internal, others external. The internal and external factors reinforce one another. The internal factors pertain to the organisational dynamics of the voluntary association itself, and the external factors to the nature of the relationship between the voluntary association concerned and public authorities and other civil society organisations. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
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Weaving cloths: Research design in contexts of transformation
- Authors: Lotz-Sisitka, Heila
- Date: 2002
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/184745 , vital:44268 , xlink:href="https://cjee.lakeheadu.ca/article/viewFile/259/136"
- Description: Through storytelling, I apply methodological and epistemological reflexivity to ask questions about the way in which environmental education research is framed in transformational settings. I ask questions about the role of research teachers/supervisors in the “weaving enterprise” or the research process. Do we have the dual task of developing contextually relevant frameworks, and of making sure that these frameworks are not adopted on an “industrial scale”—in other words, as new paradigms which have the potential to narrow research possibilities? The paper concerns itself with a broader question, notably the potentially debilitating effects of mass production of research, or the globalizing of knowledge production. The story I use to raise these, and other questions for consideration by teachers/supervisors of research is a research workers story (my own), constructed between 1992 and 1996, in a context of rapid socio-political and educational transformation in South Africa (Lotz, 1996). In addition to the above, the paper opens a debate about reflexivity as research methodological rigour.
- Full Text:
- Authors: Lotz-Sisitka, Heila
- Date: 2002
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/184745 , vital:44268 , xlink:href="https://cjee.lakeheadu.ca/article/viewFile/259/136"
- Description: Through storytelling, I apply methodological and epistemological reflexivity to ask questions about the way in which environmental education research is framed in transformational settings. I ask questions about the role of research teachers/supervisors in the “weaving enterprise” or the research process. Do we have the dual task of developing contextually relevant frameworks, and of making sure that these frameworks are not adopted on an “industrial scale”—in other words, as new paradigms which have the potential to narrow research possibilities? The paper concerns itself with a broader question, notably the potentially debilitating effects of mass production of research, or the globalizing of knowledge production. The story I use to raise these, and other questions for consideration by teachers/supervisors of research is a research workers story (my own), constructed between 1992 and 1996, in a context of rapid socio-political and educational transformation in South Africa (Lotz, 1996). In addition to the above, the paper opens a debate about reflexivity as research methodological rigour.
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Women in paid domestic work and their children : an exploratory study
- Authors: Maqungu, Nomzamo Florence
- Date: 2002 , 2013-05-16
- Subjects: Household employees -- South Africa , Women, Black -- Employment -- South Africa
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:711 , http://hdl.handle.net/10962/d1007476 , Household employees -- South Africa , Women, Black -- Employment -- South Africa
- Description: This study explores the views and experiences of domestic workers with regard to service conditions of their occupation, the arrangements they make for the care of their own children and the frequency of contact they make with their children. Data was obtained through use of pre - coded self administered questionnaires which were employed to 50 domestic women who are working in East London and its suburbs and have children under the age of 16 years. Semi-structured interviews were tape recorded and transcribed and the analysis process looked at general trends where qualitative data was obtained. Quantitative data was tabulated in frequency tables and interpreted by means of graphs. The study is pioneering efforts in the Eastern Cape and South Africa in general as well as setting a way forward for further exploration of this subject. It is hoped that the study will make a worthwhile contribution and bring upfront valuable information that could be used when addressing issues of domestic workers. The study also indirectly challenges those professions and disciplines who have been advocating on behalf of domestic workers to mobilize these workers towards taking part in the uplifting of their standards and to fInish up the good work they had already started until their aims have been achieved. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Maqungu, Nomzamo Florence
- Date: 2002 , 2013-05-16
- Subjects: Household employees -- South Africa , Women, Black -- Employment -- South Africa
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:711 , http://hdl.handle.net/10962/d1007476 , Household employees -- South Africa , Women, Black -- Employment -- South Africa
- Description: This study explores the views and experiences of domestic workers with regard to service conditions of their occupation, the arrangements they make for the care of their own children and the frequency of contact they make with their children. Data was obtained through use of pre - coded self administered questionnaires which were employed to 50 domestic women who are working in East London and its suburbs and have children under the age of 16 years. Semi-structured interviews were tape recorded and transcribed and the analysis process looked at general trends where qualitative data was obtained. Quantitative data was tabulated in frequency tables and interpreted by means of graphs. The study is pioneering efforts in the Eastern Cape and South Africa in general as well as setting a way forward for further exploration of this subject. It is hoped that the study will make a worthwhile contribution and bring upfront valuable information that could be used when addressing issues of domestic workers. The study also indirectly challenges those professions and disciplines who have been advocating on behalf of domestic workers to mobilize these workers towards taking part in the uplifting of their standards and to fInish up the good work they had already started until their aims have been achieved. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
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Workplace Reorganisation Course
- DITSELA
- Authors: DITSELA
- Date: 2002
- Subjects: Development Institute for Training,Support and Education for Labour (DITSELA)
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/175688 , vital:42606
- Description: This booklet is about the way in which work is being restructured in the workplaces of South Africa. It is the first in a series of booklets dealing with various restructuring issues faced by workers. There are major changes being made in the workplace that impact on the lives of workers. For workers, restructuring brings changes to the way that their workplace is organised and this will impact on their working lives. These changes could hold threats or opportunities for workers. This first book will explain what is meant by restructuring and look at why many companies in South Africa are considering restructuring. It will also look at some trade union strategies around restructuring and some suggestions for negotiating restructuring.
- Full Text:
- Authors: DITSELA
- Date: 2002
- Subjects: Development Institute for Training,Support and Education for Labour (DITSELA)
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/175688 , vital:42606
- Description: This booklet is about the way in which work is being restructured in the workplaces of South Africa. It is the first in a series of booklets dealing with various restructuring issues faced by workers. There are major changes being made in the workplace that impact on the lives of workers. For workers, restructuring brings changes to the way that their workplace is organised and this will impact on their working lives. These changes could hold threats or opportunities for workers. This first book will explain what is meant by restructuring and look at why many companies in South Africa are considering restructuring. It will also look at some trade union strategies around restructuring and some suggestions for negotiating restructuring.
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Workshop theatre in post-apartheid South Africa : a case study
- Authors: Copteros, Athina
- Date: 2002
- Subjects: Theater -- South Africa , Theater -- Political aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2156 , http://hdl.handle.net/10962/d1007477
- Description: This is a qualitative study exploring the use of workshop theatre in post-apartheid South Africa, with the objective of making a contribution to the knowledge-base regarding its use in current times. Workshop theatre is changing in response to a new socio-political reality and emerging trends in theatre practice. The case study, of developing a play on Oystercatchers with a Grahamstown group of artists, revealed the difficulties and challenges of using workshop theatre in this dynamic context. Data collection included a focus group, observation, reflective discussion and in-depth interviews that were analysed in relation to available literature on workshop theatre in apartheid and post-apartheid South Africa. It is proposed that workshop theatre has continued relevance in post -apartheid South Africa. The process of creating workshop theatre with diverse artists has great potential to transform relationships, address issues of personal identity and to provide an underlying purpose to a workshop theatre -making context.
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- Authors: Copteros, Athina
- Date: 2002
- Subjects: Theater -- South Africa , Theater -- Political aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2156 , http://hdl.handle.net/10962/d1007477
- Description: This is a qualitative study exploring the use of workshop theatre in post-apartheid South Africa, with the objective of making a contribution to the knowledge-base regarding its use in current times. Workshop theatre is changing in response to a new socio-political reality and emerging trends in theatre practice. The case study, of developing a play on Oystercatchers with a Grahamstown group of artists, revealed the difficulties and challenges of using workshop theatre in this dynamic context. Data collection included a focus group, observation, reflective discussion and in-depth interviews that were analysed in relation to available literature on workshop theatre in apartheid and post-apartheid South Africa. It is proposed that workshop theatre has continued relevance in post -apartheid South Africa. The process of creating workshop theatre with diverse artists has great potential to transform relationships, address issues of personal identity and to provide an underlying purpose to a workshop theatre -making context.
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