Ectomycorrhizal fungal assessment of South African Pinus patula seedlings and their biological control potential to enhance seedling growth
- FitzGerald, Véronique Chartier
- Authors: FitzGerald, Véronique Chartier
- Date: 2019
- Subjects: Ectomycorrhizal fungi , Pinus patula Diseases and pests Biological control South Africa , Fusarium , Forest nurseries , Seedlings Growth , Seedlings Diseases and pests
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/67902 , vital:29167
- Description: The South Africa forestry industry, covering 1.3 million hectares, is dependent on exotic pine and eucalyptus species. Nursery seedlings are not inoculated with ectomycorrhizal (ECM) fungi or other beneficial microbes. Fusarium circinatum is an economically important pathogen affecting seedling survival. The purpose of this investigation was to assess levels of naturally occurring ECM colonisation in South African nurseries and to determine the effects of seedling inoculation with selected ECM and bacterial isolates on plant growth and resistance to the fungal pathogen F. circiantum. Pinus patula seedlings from 10 different South African nurseries were assessed for ECM colonisation using a grid line intersect method and molecularly identified using morphological and next-generation Illumina sequencing. Explants from ECM basidiocarps, collected from Pinus stands, were plated onto MMN medium to obtain isolates which were verified using molecular techniques. Mycorrhizal helper bacteria (MHB) were also isolated from these basiocarps, tested for MHB properties, siderophore production, phospahte solubilising and IAA production. ECM and associated bacterial isolates were used to inoculate seedlings and growth was assessed over a 5 month period. Colonisation of seedlings in production nurseries was low (2-21%). Morphologically the ECM fungi T. terrestris, Suillus sibiricus, and the genera Russula, Pseudotomentella were identified. Molecularly the ECM fungi T. terrestris, Inocybe jacobi and the genera Sphaerosporella and several other ECM containing families were identified along with many saprotrophic/endophytic fungi belonging to genera such as, Penicillium, Ramasonia and Talaromyces. Inoculated seedlings showed a significant increase in growth in comparison to the un-inoculated control seedlings. ECM fungal colonisation levels of these seedlings were significantly increased and colonisation was promoted by the Suillus isolate, Salmon Suillus. Seedling growth in the presence of the pathogen F. circinatum was significantly increased and promoted by the Lactarius isolate Lactarius quieticolor. Inoculation of seedlings in the nursery would ensure the production of stronger healthy plants which may be more tolerant to fusarial infection increasing survival in the plantation. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2019
- Full Text:
- Date Issued: 2019
- Authors: FitzGerald, Véronique Chartier
- Date: 2019
- Subjects: Ectomycorrhizal fungi , Pinus patula Diseases and pests Biological control South Africa , Fusarium , Forest nurseries , Seedlings Growth , Seedlings Diseases and pests
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/67902 , vital:29167
- Description: The South Africa forestry industry, covering 1.3 million hectares, is dependent on exotic pine and eucalyptus species. Nursery seedlings are not inoculated with ectomycorrhizal (ECM) fungi or other beneficial microbes. Fusarium circinatum is an economically important pathogen affecting seedling survival. The purpose of this investigation was to assess levels of naturally occurring ECM colonisation in South African nurseries and to determine the effects of seedling inoculation with selected ECM and bacterial isolates on plant growth and resistance to the fungal pathogen F. circiantum. Pinus patula seedlings from 10 different South African nurseries were assessed for ECM colonisation using a grid line intersect method and molecularly identified using morphological and next-generation Illumina sequencing. Explants from ECM basidiocarps, collected from Pinus stands, were plated onto MMN medium to obtain isolates which were verified using molecular techniques. Mycorrhizal helper bacteria (MHB) were also isolated from these basiocarps, tested for MHB properties, siderophore production, phospahte solubilising and IAA production. ECM and associated bacterial isolates were used to inoculate seedlings and growth was assessed over a 5 month period. Colonisation of seedlings in production nurseries was low (2-21%). Morphologically the ECM fungi T. terrestris, Suillus sibiricus, and the genera Russula, Pseudotomentella were identified. Molecularly the ECM fungi T. terrestris, Inocybe jacobi and the genera Sphaerosporella and several other ECM containing families were identified along with many saprotrophic/endophytic fungi belonging to genera such as, Penicillium, Ramasonia and Talaromyces. Inoculated seedlings showed a significant increase in growth in comparison to the un-inoculated control seedlings. ECM fungal colonisation levels of these seedlings were significantly increased and colonisation was promoted by the Suillus isolate, Salmon Suillus. Seedling growth in the presence of the pathogen F. circinatum was significantly increased and promoted by the Lactarius isolate Lactarius quieticolor. Inoculation of seedlings in the nursery would ensure the production of stronger healthy plants which may be more tolerant to fusarial infection increasing survival in the plantation. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2019
- Full Text:
- Date Issued: 2019
Towards a biological profile for South African perinatal remains: osteological and genetic perspectives
- Authors: Thornton, Roxanne
- Date: 2019
- Subjects: Identification , Forensic osteology , Methylation , RNA , Autopsy
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/68102 , vital:29198 , DOI 10.21504/10962/68102
- Description: Forensic identification of abandoned and suspected infanticide cases admitted to the South African Forensic Pathology Services is often impossible due to decomposition of the remains. In these cases, investigation of suspected criminal activity is almost never pursued. Ancillary tests in the form of anthropological and molecular analyses can assist with the forensic identification of perinatal remains. To provide fundamental information about bone development of perinatal skeleton, osteological and genetic techniques focusing on the pars basilaris, pars lateralis, sternal rib and left femur were used. Samples were obtained from unidentified and unclaimed remains originating from the Johannesburg Forensic Pathology Service (JFPS). To provide a biological age to individuals in the collection, dental aging was used to categorize remains for comparisons with anthropological and molecular data. A molecular protocol was designed to sex individuals using the X-linked G6PD and Y-linked SRY genes. Bone development was studied using osteometric and morphological data of dry bone remains coupled with bone mineral density analysis (Micro-CT). The methylation levels of CpG rich sites within the promoter region of selected bone-associated genes were incorporated to examine silencing of genes during development. Osteological results support the use of the pars basilaris, pars lateralis and femur for age-at-death estimations as well as provide the foundation for dry bone aging criteria for South African individuals. Data compared with established skeletal aging standards indicated developmental differences between populations. Through the use of animal models and the perinatal sternal rib tissue, insights and precautions into the use of post mortem bone derived RNA for forensic applications is communicated. The methylation status of CpG rich sites within the promoter regions support the hypothesis for interdependent machinery involving selected genes during early bone development. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2019
- Full Text:
- Date Issued: 2019
- Authors: Thornton, Roxanne
- Date: 2019
- Subjects: Identification , Forensic osteology , Methylation , RNA , Autopsy
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/68102 , vital:29198 , DOI 10.21504/10962/68102
- Description: Forensic identification of abandoned and suspected infanticide cases admitted to the South African Forensic Pathology Services is often impossible due to decomposition of the remains. In these cases, investigation of suspected criminal activity is almost never pursued. Ancillary tests in the form of anthropological and molecular analyses can assist with the forensic identification of perinatal remains. To provide fundamental information about bone development of perinatal skeleton, osteological and genetic techniques focusing on the pars basilaris, pars lateralis, sternal rib and left femur were used. Samples were obtained from unidentified and unclaimed remains originating from the Johannesburg Forensic Pathology Service (JFPS). To provide a biological age to individuals in the collection, dental aging was used to categorize remains for comparisons with anthropological and molecular data. A molecular protocol was designed to sex individuals using the X-linked G6PD and Y-linked SRY genes. Bone development was studied using osteometric and morphological data of dry bone remains coupled with bone mineral density analysis (Micro-CT). The methylation levels of CpG rich sites within the promoter region of selected bone-associated genes were incorporated to examine silencing of genes during development. Osteological results support the use of the pars basilaris, pars lateralis and femur for age-at-death estimations as well as provide the foundation for dry bone aging criteria for South African individuals. Data compared with established skeletal aging standards indicated developmental differences between populations. Through the use of animal models and the perinatal sternal rib tissue, insights and precautions into the use of post mortem bone derived RNA for forensic applications is communicated. The methylation status of CpG rich sites within the promoter regions support the hypothesis for interdependent machinery involving selected genes during early bone development. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2019
- Full Text:
- Date Issued: 2019
An anthropological investigation into the challenges that affect Xhosa traditional circumcision: a case of Mngqesha Village, Buffalo City Metropolitan Municipality
- Authors: Qegu, Mzukisi
- Date: 2018-06
- Subjects: Circumcision , Xhosa (African people)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26683 , vital:65851
- Description: The purpose of this study is to investigate the challenges that affect Xhosa traditional circumcision in Mngqesha village. Mngqesha village is situated in Dimbaza, Eastern Cape Province, Republic of South Africa. The study population employed random and purposive sampling to draw a representative sample of 25 research informants traditional leaders, traditional surgeons, traditional nurses, Initiates, elders of the community and Department of Health officials, each group was represented by five research informants from the population of this study. This study employed a mixed research approach. Questionnaires were distributed amongst research informants for quantitative purposes, and interviews were conducted for qualitative purposes, among the selected representative sample. Qualitative data was analyzed using thematic analysis and was used to substantiate quantitative findings. The findings of this study prolonged on how injuries, diseases and high death rates of initiates affect Xhosa Traditional Circumcision in Mngqesha village. The findings of this study divulge that carelessness amongst parents of initiates, dehydration, physical abuse and lack of proper screening of initiates have a tremendous contribution to injuries, diseases and high death rates of initiates in Mngqesha villages. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2018
- Full Text:
- Date Issued: 2018-06
- Authors: Qegu, Mzukisi
- Date: 2018-06
- Subjects: Circumcision , Xhosa (African people)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26683 , vital:65851
- Description: The purpose of this study is to investigate the challenges that affect Xhosa traditional circumcision in Mngqesha village. Mngqesha village is situated in Dimbaza, Eastern Cape Province, Republic of South Africa. The study population employed random and purposive sampling to draw a representative sample of 25 research informants traditional leaders, traditional surgeons, traditional nurses, Initiates, elders of the community and Department of Health officials, each group was represented by five research informants from the population of this study. This study employed a mixed research approach. Questionnaires were distributed amongst research informants for quantitative purposes, and interviews were conducted for qualitative purposes, among the selected representative sample. Qualitative data was analyzed using thematic analysis and was used to substantiate quantitative findings. The findings of this study prolonged on how injuries, diseases and high death rates of initiates affect Xhosa Traditional Circumcision in Mngqesha village. The findings of this study divulge that carelessness amongst parents of initiates, dehydration, physical abuse and lack of proper screening of initiates have a tremendous contribution to injuries, diseases and high death rates of initiates in Mngqesha villages. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2018
- Full Text:
- Date Issued: 2018-06
Assessment of the role of continuing professional development on the transformation of practice: a case study of the Advanced Diploma in District Health Management and Leadership curriculum
- Authors: Ndawule, Luvuyo
- Date: 2018-06
- Subjects: Continuing education , Teachers -- Training of -- South Africa , Active learning
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26672 , vital:65848
- Description: This study is located in the field of education, within the domain of Continuing Professional Development CPD and curriculum. The study sought to assess the role of CPD in the transformation of health practice. The media’s complaints about poor service delivery within spheres of government, from local up to national level, triggered interest in a study of this nature. Since the Department of Health falls amongst the essential services, research on current public healthcare with the intention of transforming its practice was seen as imperative. An Advanced Diploma in District Health Management and Leadership ADDHM & L offered by Health Sciences at the University of Fort was used as a case. The healthcare practitioners who attended the stated programme were studied as a second case. The setting is in the public health care centres in the Department of Health within the Amathole region and Nelson Mandela Bay Metropolitan Municipality. An interpretive paradigm was used to ordain the study, the three sets of data were collected from various categories of participants such as graduates of the ADDHM & L programme, their workplace supervisors, as well as the facilitators of the programme. The last set of data was collected from the ADDHM & L documents. Interviews and questionnaires were used as data collection tools and thematic analysis was used for data analysis. The findings revealed that curriculum development and design has a strong bearing on formal continuing professional development programmes. For such programmes to be able to transform practice, the curriculum pedagogies should be designed and developed in such a way that the learning taking place is authentic in nature. It is this type of learning that will influence reflective practice and translation of knowledge. The findings also revealed that institutions of higher learning do play a crucial role in closing the gap that exists between theory and practice through designing programmes that are pedagogically aligned towards authentic learning. The learning instructions, the experiences, the expectations of the learner, the assessment forms that influence the nature of tasks and activities given by facilitators can either enable or hinder the ability to transform practice. , Thesis (PhD) -- Faculty of Education, 2018
- Full Text:
- Date Issued: 2018-06
- Authors: Ndawule, Luvuyo
- Date: 2018-06
- Subjects: Continuing education , Teachers -- Training of -- South Africa , Active learning
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26672 , vital:65848
- Description: This study is located in the field of education, within the domain of Continuing Professional Development CPD and curriculum. The study sought to assess the role of CPD in the transformation of health practice. The media’s complaints about poor service delivery within spheres of government, from local up to national level, triggered interest in a study of this nature. Since the Department of Health falls amongst the essential services, research on current public healthcare with the intention of transforming its practice was seen as imperative. An Advanced Diploma in District Health Management and Leadership ADDHM & L offered by Health Sciences at the University of Fort was used as a case. The healthcare practitioners who attended the stated programme were studied as a second case. The setting is in the public health care centres in the Department of Health within the Amathole region and Nelson Mandela Bay Metropolitan Municipality. An interpretive paradigm was used to ordain the study, the three sets of data were collected from various categories of participants such as graduates of the ADDHM & L programme, their workplace supervisors, as well as the facilitators of the programme. The last set of data was collected from the ADDHM & L documents. Interviews and questionnaires were used as data collection tools and thematic analysis was used for data analysis. The findings revealed that curriculum development and design has a strong bearing on formal continuing professional development programmes. For such programmes to be able to transform practice, the curriculum pedagogies should be designed and developed in such a way that the learning taking place is authentic in nature. It is this type of learning that will influence reflective practice and translation of knowledge. The findings also revealed that institutions of higher learning do play a crucial role in closing the gap that exists between theory and practice through designing programmes that are pedagogically aligned towards authentic learning. The learning instructions, the experiences, the expectations of the learner, the assessment forms that influence the nature of tasks and activities given by facilitators can either enable or hinder the ability to transform practice. , Thesis (PhD) -- Faculty of Education, 2018
- Full Text:
- Date Issued: 2018-06
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