Having a phone, having style:
- Authors: Schoon, Alette
- Date: 2012
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/159506 , vital:40303 , https://hdl.handle.net/10520/EJC135828
- Description: It was dusk, and a group of young men with a dog trailing behind them, were walking past on a dusty township road in Hooggenoeg, an RDP village on the rim of the Grahamstown bowl. While they ambled along, one was holding up a mobile phone on which music was playing, dancing while he walked. This was exactly the kind of thing I was hoping to observe in my new identity as researcher and participant observer, and was why I had agreed to judge the Hooggenoeg fashion show which turned out to be more of a beauty competition. While I'm not generally fond of these kind of shows and the way they essentialise beauty, I agreed because I wanted to get more of a sense of how mobile phones are integrated into the everyday lives of these young adults.
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- Date Issued: 2012
Henry Williams : The Glasbury Bellfounder, and the production and tuning of bells
- Authors: Lewis, Colin A
- Date: 2012
- Language: English
- Type: Book chapter
- Identifier: vital:6159 , http://hdl.handle.net/10962/d1004779 , Full text available to the end of Chapter 2
- Description: The production and tuning of church bells is described before attention is paid to Henry Williams, the Glasbury bellfounder who died in 1722, and his bells. The bells of TP, his apparent successor are also described. Henry Williams was probably a member of the Williams family of Gwernyfed and was born c 1635. His presumed family connections are described. Bells known to have been cast by Henry Williams between 1677 and 1719 are listed; their distribution depicted; and their qualities assessed. The inventory of Williams' possessions at the time of his death is presented and discussed. Bells cast by TP during a brief foray into bell founding, apparently 1738-40, are described and their qualities assessed. TP was probably Benjamin Tanner (T), the industrialist specialising in iron production who succeeded Williams at the Pipton forge (P) in Aberllynfi parish near Glasbury, or Tanner (T) and Samuel Prichard (P). Prichard was the grandson of Henry Williams and his sole executor. , A complete copy of this book is available at The Whiting Society of Ringers at: http://www.whitingsociety.org.uk
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- Date Issued: 2012
High pressure liquid chromatographic quantification of nitrile biocatalysis
- Authors: Mathiba, Kgama
- Date: 2012
- Subjects: High performance liquid chromatography , Rhodococcus , Biocatalysis , Organic compounds -- Industrial applications
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4129 , http://hdl.handle.net/10962/d1015710
- Description: Nitrile biocatalysts are of use in the chemical and pharmaceutical industries for the synthesis of carboxyamides and carboxylic acids. In particular, the application of biocatalysts in the synthesis of single enantiomer compounds is of increasing interest, but requires novel substrate specific highly stereoselective biocatalysts. Addition to the limited toolbox of known nitrile biocatalysts requires definitive characterisation of the biocatalysts through accurate determination of the substrate profiles and quantification of activity. The accurate quantification of stereoisomers chiral mixtures to determine biocatalyst stereoselectivity remains a significant challenge due to the difficulty in separating stereoisomers by physical methods. The known nitrile metabolising organism, Rhodococcus rhodochrous ATCC BAA-870, was grown in a defined medium and harvested, providing whole cell biocatalyst. Additional biomass was disrupted to provide a cell free enzyme extract, which was put through an enzyme purification protocol to provide a solution with specific activity of 351 U.mg⁻¹. A portion of the enzyme was self immobilised using the SphereZyme™ technique. The nitrile hydratase SphereZymes™ (1.2 U.mg⁻¹ initial activity) that were prepared had pH and temperature optima of 6 and 30°C respectively, and could be recovered by repeated washing. The particles retained activity in the presence of the organic solvents isooctane and n-hexadecane saturated with 50 mM phosphate buffer (pH 7.5). An initial analytical system was devised for quantification of the nitrile hydratase activity using the non-chiral substrate benzonitrile. An improved reversed phase high performance liquid chromatography method was developed to separate and quantify benzamide, benzoic acid and benzonitrile. The mobile phase consisting of 0.1% trifluoroacetic acid in H₂O and acetonitrile (70:30, %v/v), at a flow rate of 0.5 ml.ml⁻¹, 25°C, resolved all three analytes in 3.5 minutes on a Waters X-Terra MS C18 3.5μm column. UV detection was carried out at 210 nm. Analytical methods to determine activity and enantioselectivity of the whole cell biocatalyst were subsequently developed for both β-amino nitriles and β-hydroxy nitrile substrates and hydrolysis products.
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- Date Issued: 2012
Higher education, academic communities, and the intellectual virtues
- Authors: Jones, Ward E
- Date: 2012
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/275647 , vital:55066 , xlink:href="https://doi.org/10.1111/edth.12005"
- Description: Because higher education brings members of academic communities in direct contact with students, the reflective higher education student is in an excellent position for developing two important intellectual virtues: confidence and humility. However, academic communities differ as to whether their members reach consensus, and their teaching practices reflect this difference. In this essay, Ward Jones argues that both consensus-reaching and non-consensus-reaching communities can encourage the development of intellectual confidence and humility in their students, although each will do so in very different ways.
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- Date Issued: 2012
Histopathological changes in male wistar rats maintained on a water-based sutherlandia frutescens extract
- Authors: Wickens, Nicolas John
- Date: 2012
- Subjects: Rats -- Physiology , Rats as laboratory animals
- Language: English
- Type: Thesis , Doctoral , DTech
- Identifier: http://hdl.handle.net/10948/4742 , vital:20670
- Description: In this study a standardized 46 week chronic drinking water toxicity protocol was used to elucidate the toxic potential of Sutherlandia frutescens (S. frutescens) using histopathologic, morphometric and transmission electron microscopic analysis. The histopathologic changes in the duodenum, heart, kidney, liver, lung, pancreas and spleen of male Wistar rats were evaluated. Fifty-four rats were randomly divided into four groups: Group 1 – Normal diet control (ND control), n=7, Group 2 – Normal diet + plant extract (ND + p), n=9, Group 3 – High fat diet control (HFD control), n=19Group 4 – High fat diet + p (HFD + p), n=19In the high fat group male Wistar rats were fed ±55 g/day of a specialised high fat diet over a 46 week period to induce obesity and an insulin resistant state. The treatment groups (groups 2 and 4) received a dose concentration of a tea extract of the S. frutescens plant in their drinking water daily. This study showed that the consumption of S. frutescens significantly reduces weight gain in male Wistar rats on a chronic high fat diet (p≤0.001 vs. HFD control group). S. frutescens appears to propagate periportal and centrilobular glycogen storage in rat hepatocytes in the experimental groups as exemplified by a significantly (p≤0.0001 vs. control groups) increased incidences of Periodic Acid Schiff (PAS) positive staining S. frutescens also reduced intracellular lipid accumulation as made evident by the significantly lower incidence of epicardial adipose tissue (EAT), hepatic steatosis and pancreatic interstitial fat. Obesity was associated with increased fibrotic lesions such as myocardial perivascular fibrosis, centrilobular hepatic fibrosis and pancreatic periductal fibrosis. Obesity associated hypertension contributed to the widespread and significant increase in the average lesion severity of arterial congestion in all organs in the HFD control group. Pulmonary infection was equally prevalent in all rats. Despite the complex histopathology in all groups, differences in the control groups, such as, the presence of a conservative polymorphonuclear leukocyte (PMNL) infiltration, substantial intra-alveolar oedema and focal arterial wall hypertrophy in the control groups was highly suggestive of Sendai viral infection. However histopathologic evidence, in the treatment groups, suggested chronic recurrent viral infection with superimposed Mycoplasma pulmonis (M. pulmonis) bacterial infection. The impact of advanced suppurative pulmonary infection was widespread and exemplified by increased lesion incidences of spontaneous murine progressive cardiomyopathy (MCP) and spontaneous chronic progressive nephropathy (CPN) among others. In conclusion S. frutescens administered for 46 weeks to male Wistar rats significantly lowered intracellular lipid accumulation and obesity associated myocardial, renal, hepatobiliary, pulmonary and pancreatic histopathology. Moreover, duodenal, cardiovascular, hepatobiliary, pulmonary, renal, pancreatic and splenic tissue did not show histopathologic evidence of direct plant extract associated toxicity or carcinogenicity.
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- Date Issued: 2012
Hiv/Aids communication and youth behaviour in South Africa: a study of female high school students in the Eastern Cape Province
- Authors: Mpofu, Nkosinothando
- Date: 2012
- Subjects: HIV infections -- Social aspects -- South Africa , AIDS (Disease) -- Social aspects -- South Africa , Communication in public health -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD (Com)
- Identifier: vital:11368 , http://hdl.handle.net/10353/d1015374 , HIV infections -- Social aspects -- South Africa , AIDS (Disease) -- Social aspects -- South Africa , Communication in public health -- South Africa
- Description: Human Immuno-Deficiency Virus and Acquired Immune-Deficiency Syndrome communication remains one of the most significant tools in the fight against HIV/AIDS given the absence of the cure to fight the growth and spread of the global pandemic. Through the use of information, HIV/AIDS communication seeks to empower recipients or societies with skills that will help reduce their risk of infection. South Africa has seen a high visibility of HIV/AIDS communication programmes or campaigns aimed at empowering different audiences, whilst paying particular emphasis on the most vulnerable. Young people, in particular young women, have a higher prevalence of HIV/AIDS in South Africa. This has prompted the development of many youth focused communication campaigns which have sought to address factors that increase young people`s vulnerability to HIV infection. However, despite the high visibility of HIV/AIDS communication campaigns targeting young people, high risk behaviours are still being seen among young women. Important to note are the high teenage pregnancy rates, growing abuse of substances and even the premature engagement of sexual activities among female youths. This, therefore, raises questions on the effect of HIV/AIDS communication programmes in encouraging protective behaviour against risky behavioural practices amongst young women. Using both quantitative and qualitative research approaches to this study, an investigation was conducted into whether current HIV/AIDS communication campaigns have been limited (in terms of effectiveness) when it comes to communicating with young women on issues relating to HIV/AIDS. Awareness of HIV/AIDS and HIV/AIDS communication programmes, relevance of HIV/AIDS communication programmes, the factors that influence the use and understanding of HIV/AIDS messages and the impact of HIV/AIDS communication on attitude and behaviour change amongst female youths is measured. A total of 350 questionnaire copies were self-administered to 350 participants, with a 100 percent response rate. From the 350 participants, seventy five took part in focus group discussions. Data obtained was analysed using SPSS (for descriptive statistics), and the grounded theory. The results of the study indicate that all participants were aware of HIV/AIDS with at least 60% of the participants aware of at least three HIV/AIDS communication campaigns. When measuring the relevance of HIV/AIDS communication campaigns, participants (58%) indicate that issues discussed in most HIV/AIDS communication campaigns increased the relevance of HIV/AIDS messages to young women although such relevance was, for some respondents, affected by limited access to communication campaigns. Results also show that levels of knowledge and understanding of factors that expose young women to HIV infections differed amongst participants in as much as the factors that hinder the use of advice contained in HIV/AIDS messages also differed. Sixty-nine percent of the participants have knowledge and a better understanding of factors that expose young females to HIV infections. Twenty-five percent of the participants identified peer pressure, whilst 23% identified limited access to HIV/AIDS communication campaigns and another 18% identified a disregard of HIV/AIDS messages as significant factors that limit the ability of individuals to implement advice contained in HIV/AIDS messages. The results also indicate that although HIV/AIDS communication campaigns seem to have played a role in empowering some participants, there is still a significant minority whom communication has not effectively communicated with. Based on these findings, the study suggests, amongst other things, the need to intensify current HIV/AIDS communication campaigns through the provision of consistent messages on appropriate condom use, the identification of easily accessible communication channels and the development of thought provoking and attention grabbing campaigns as well as the need to continue to directly involve young women in the processes of their own development.
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- Date Issued: 2012
Holistic juvenile justice centre: the design of a one-stop juvenile centre in Windhoek, Namibia
- Authors: Saunders, Dylan
- Date: 2012
- Subjects: Juvenile detention homes -- Namibia -- Windhoek -- Designs and plans Juvenile detention homes -- Buildings, structures, etc..
- Language: English
- Type: Thesis , Masters , MArch
- Identifier: http://hdl.handle.net/10948/39144 , vital:35048
- Description: The promotion and protection of children's rights has since independence been a 'political commitment within the country to improve the situation of children1.Namibia has ratified the Convention on the Rights of the Child, whilst the Child Care and Protection Bill has been submitted to the Cabinet Committee on Legislation in 20102. Both seek to protect the rights of the child through a holistic approach. However, scepticism and uncertainty still pervades amongst the involved professional fields3. This can be attributed to the history of war and apartheid in Namibia, as well as social pressures of poverty and colonial legislation. Despite the adoption of national legislation respecting children's rights, difficulties in implementing the required procedural framework have placed Namibia at odds with international precedent4. In contrast, South Africa has developed specialised One-Stop Youth Justice Centres intended to promote the efficient operation of the juvenile legal system as part of promoting the rights of children5. Relying on inter-departmental cooperation, these centres are able to render comprehensive services to children in conflict with the law. Children are guided through the legal process after their arrest with the intended result of successful rehabilitation and integration into society. The main goal is to ensure that children are kept out of the conventional criminal justice system and only if rehabilitation has failed, will a child be sent to prison where a traditional sentence will be served. To better effect this these centres are to be located in residential areas. In light of international practises, courthouses are increasingly developing as specialised buildings tailored to the provision of the necessary services relevant to a particular type of civic or criminal offense6. Drawing a parallel to this, One-Stop Centres do not accommodate adult offenders, these centres are focussed on providing the necessary services to juvenile offenders and witnesses and victims of crimes committed by juveniles. This type of 'childfriendly' one-stop approach has unfortunately not permeated into the practices of the Namibian criminal justice system7. It is within this context that the design treatise has been investigated.
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- Date Issued: 2012
How community participation can be enhanced in the creation of sustainable human settlements in Whittlesea : a case study of Whittlesea Mabuyase housing project
- Authors: Penxa, Lungile
- Date: 2012
- Subjects: Political participation -- South Africa -- Eastern Cape , Human settlements -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9211 , http://hdl.handle.net/10948/d1020772
- Description: Development is an idea, an objective and an activity (Kothari and Minogue, 2002). Development emerged as an idea that nations had to adopt in their journey through post-war history. Development is seen as an ambiguous concept (Allen and Thomas, 1992: 23). In other words, it is defined in many ways to suit different purposes. Furthermore, over the long term development implies increased living standards, improved health and well being for all, and the achievement of whatever is regarded as a general good for society at large (Allen and Thomas, 1992: 23). Therefore, from the above development appears as a “one size fits all” concept, because it is an attempt to address the problems in society. Furthermore, development has been defined as a participatory, people-centred process intended to reduce the incidence of poverty and achieve better livelihoods for all (Kingsbury, McKay, and Hunt, 2004: 43). The definition of development as participatory and people-centred is the most applicable for the purposes of this study. Participation has been widely used in the development discourse. Within the development sphere, participation has been associated with the community sector (Gaventa and Valderrama, 1999:2). The definition of participation in development has been located in development projects and programmes (i.e. sustainable human settlements for our study purposes), as a means of strengthening their relevance, quality and sustainability (Gaventa and Valderrama, 1999:2). Strengthening of participation in local governance has to do with the strengthening of directcitizen involvement in decision-making by individuals or groups in public activities, oftenthrough newly established institutional channels, such as monitoring committees, planning processes, etc (Gaventa and Valderrama, 1999:8-9). Hence, participation could be seen as involvement in decision-making in all phases of a project (Gaventa and Valderrama, 1999:2). Citizen participation is about power and its exercise by different social actors in the spacescreated for the interaction between citizens and local authorities. However, the control of thestructure and processes for participation - defining spaces, actors, agendas, procedures - is usuallyin the hands governmental institutions and can become a barrier for effective involvement ofcitizens (Gaventa and Valderrama, 1999:7). The above discussion has been an attempt to show what participatory development or participation in a development process is all about. This has been done through briefly defining and discussing development and participation and also showing the relationship between the two concepts. Now the discussion will focus on enlightening the reader about sustainable human settlements – the main issue of this study. The decision to do research in this area came after the realization that there is still a shortage of houses in the Eastern Cape, and when the government does deliver these houses in a particular area people still have complaints regarding the new houses delivered to them. Then a question that came to mind was whether people are consulted or not before these houses are constructed during the planning stages so that they can give their own views or ideas on houses. Overall the reason for choosing this topic was to look at people’s participation on the development of houses. It is evident in the Provincial Medium Term Sustainable Human Settlement Research Agenda that community participation is lacking in the housing delivery process of the Eastern Cape (Province of the Eastern Cape Human Settlement,2011:9).
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- Date Issued: 2012
How do HIV and AIDS impact the use of natural resources by poor rural populations?: The case of wild animal products
- Authors: Kaschula, Sarah , Shackleton, Charlie M
- Date: 2012
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/182344 , vital:43822 , xlink:href="https://hdl.handle.net/10520/EJC97203"
- Description: As a result of heightened financial and food insecurity, populations adversely affected by HIV and/or AIDS may be more likely to utilise wild natural resources to supplement their diet and livelihoods. Should this effect be pronounced, HIV and AIDS may pose a serious environmental threat. We explored the hypothesis that the presence of factors in the household, such as chronic illness in and recent mortality of individuals in a high HIV-risk age group, as well as the fostering of orphans, are associated with increased utilisation of wild animal products (WAPs) at the household level. We randomly surveyed 519 households from four sites in rural South Africa, recording household socio-economic status, the utilisation of wild animal products and health and demographic factors attributed to HIV or AIDS. Binary logistic regressions were used to test if households with markers of HIV and/or AIDS affliction were more likely to have a higher incidence and frequency of WAP utilisation relative to non-afflicted households, after adjusting for socio-economic and demographic variables. We found that, although households with markers of HIV and/or AIDS were generally poorer and had higher dependency ratios, there was no evidence to support the hypothesis that WAP harvesting was associated with either poverty, or markers of HIV and/or AIDS affliction. Our findings suggest that generalisations about a possible interaction between HIV and/or AIDS and the environment may not uniformly apply to all categories of natural resources or to all user groups.
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- Date Issued: 2012
How does the ungulate community respond to predation risk from cheetah (Acinonyx jubatus) in Samara Private Game Reserve?
- Authors: Makin, Douglas Ferguson
- Date: 2012
- Subjects: Ungulates -- South Africa -- Eastern Cape , Predatory animals -- Ecology -- South Africa -- Eastern Cape , Predation (Biology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10722 , http://hdl.handle.net/10948/d1018461
- Description: Predator reintroductions are becoming increasingly more common for multiple reasons, including assisting with the conservation of a predator species, the restoration of ecosystem functions and the economic benefits of their reintroduction for ecotourism ventures. There remains however, little knowledge on prey species responses to these predator reintroductions. As such, the reintroduction of cheetah (Acinonyx jubatus) into Samara Private Game Reserve provided an opportunity to investigate prey responses to predator reintroduction across a range of spatial and temporal scales. More specifically, the aim of this study was to investigate the effect of cheetah predation risk on habitat use and behavioural responses of the resident ungulate community. Samara is divided into “predator present” and “predator absent” sections, providing the opportunity to conduct a comparative study investigating the effect of cheetah on prey responses. It was hypothesized that different ungulate species would respond differently to the presence of cheetah, depending on differences in perceived vulnerability to cheetah predation. To address this, shifts in habitat use, and behavioural responses of the ungulate community reflected at landscape and patch scale were investigated. Overall, ungulate species have not shifted habitat use since cheetah reintroduction, this was possibly related to life history strategy constraints and the need for individuals to obtain suitable forage and therefore remain in specific habitats. While no shift in habitat use was observed for the majority of ungulate species, a shift in behaviour was observed for kudu (Tragelaphus strepsiceros) at a landscape scale, where kudu dedicated more time to vigilance and less time to foraging within the predator section. In addition, individuals within smaller kudu groups were observed to be more vigilant than individuals within larger groups of kudu within the predator section. Although the other three ungulate species monitored did not increase time spent vigilant within the predator section, they still maintained relatively high levels of vigilance, potentially as a means of social monitoring. At a patch level, ungulate species responded strongly to a predator cue as a proxy for cheetah proximity, and increased time spent vigilant with a trade-off of lower foraging effort. This vigilant response was strongest for kudu. Differences in perceived predation risk were reflected within eland (Tragelaphus oryx) and kudu species demographic classes, with juvenile eland and kudu, adult female kudu and subadult female kudu spending more time vigilant within manipulated patches than respective males of each species. Kudu were also observed adopting fine-scale behavioural responses to minimize predation risk within patches. The asymmetrical prey species response to perceived predation risk from cheetah supports the hypothesis that different species respond differently to the presence of a predator. Furthermore, this study illustrated the importance of measuring prey responses to predation risk across multiple scales and highlighted the need to replicate this study for a number of different sites where predators have been reintroduced, to better understand the range of factors influencing these predator-prey interactions.
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- Date Issued: 2012
How female street vendors in Nelson Mandela Bay are organized
- Authors: Tyida, Vuyokazi Andisiwe
- Date: 2012
- Subjects: Street vendors -- South Africa -- Nelson Mandela Bay , Businesswomen -- South Africa -- Nelson Mandela Bay , Merchants -- South Africa -- Nelson Mandela Bay
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9106 , http://hdl.handle.net/10948/d1012674 , Street vendors -- South Africa -- Nelson Mandela Bay , Businesswomen -- South Africa -- Nelson Mandela Bay , Merchants -- South Africa -- Nelson Mandela Bay
- Description: As one of the most impoverished provinces in South Africa with a high unemployment rate the Eastern Cape (Stats SA, 2011) was more suited for a study of this nature. In executing the research aims and objectives the study will be limited to focus specifically on one of the provinces cities, the Nelson Mandela Bay. The investigation will be focused on women street vendors mainly trading with fruits and vegetables, as well as other perishables. This industry was chosen because according to the Labour Force Survey (2000), there was an estimated 500,000 street traders operating in South Africa and more than 70 percent of them were found to be dealing in the trade of food items (Motala, 2002). The focus on women was driven by mainly by the fact that they constitute a majority of the street traders that are involed in the sale perishables/food items (Skinner, 2008).
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- Date Issued: 2012
Human rights and post-conflict peace building in the Democratic Republic of Congo
- Authors: Juma, Laurence
- Date: 2012
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/128438 , vital:36109 , https://0-hdl.handle.net.wam.seals.ac.za/10520/EJC192855
- Description: This article deals with normative issues that arose in the post-conflict peace-building processes in the Democratic Republic of Congo (DRC). It undertakes an historical review of the major episodes in the DRC peace process and highlights how these episodes yielded to the establishment of the interim government in 2003 and the enactment of the current constitution. It mainly highlights the proprietary consequences of the Lusaka peace Accord, the transformative aspects of the 2005 Constitution and the role that international organs, especially the ICC, have played in consolidating peace in the DRC.
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- Date Issued: 2012
Hydrological uncertainty analysis and scenario-based streamflow modelling for the Congo River Basin
- Authors: Tshimanga, Raphael Muamba
- Date: 2012
- Subjects: Congo River -- Regulation Hydrological surveys -- Congo River Watershed Water resources development -- Congo River Watershed Water resources development -- Environmental aspects -- Congo River Watershed Water supply -- Congo River Watershed River engineering -- Congo River Watershed
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6029 , http://hdl.handle.net/10962/d1006158
- Description: The effects of climate and environmental change are likely to exacerbate water stress in Africa over the next five decades. It appears obvious, therefore, that large river basins with considerable total renewable water resources will play a prominent role in regional cooperation to alleviate the pressure of water scarcity within Africa. However, managing water resources in the large river basins of Africa involves problems of data paucity, lack of technical resources and the sheer scale of the problem. These river basins are located in regions that are characterized by poverty, low levels of economic development and little food security. The rivers provide multiple goods and services that include hydro-power, water supply, fisheries, agriculture, transportation, and maintenance of aquatic ecosystems. Sustainable water resources management is a critical issue, but there is almost always insufficient data available to formulate adequate management strategies. These basins therefore represent some of the best test cases for the practical application of the science associated with the Predictions in Ungauged Basins (PUB). The thesis presents the results of a process-based hydrological modelling study in the Congo Basin. One of the primary objectives of this study was to establish a hydrological model for the whole Congo Basin, using available historical data. The secondary objective of the study was to use the model and assess the impacts of future environmental change on water resources of the Congo Basin. Given the lack of adequate data on the basin physical characteristics, the preliminary work consisted of assessing available global datasets and building a database of the basin physical characteristics. The database was used for both assessing relationships of similarities between features of physiographic settings in the basin (Chapters 3 and 4), and establishing models that adequately represent the basin hydrology (Chapters 5, 6, and 7). The representative model of the Congo Basin hydrology was then used to assess the impacts of future environmental changes on water resources availability of the Congo Basin (Chapter 8). Through assessment of the physical characteristics of the basin, relationships of similarities were used to determine homogenous regions with regard to rainfall variability, physiographic settings, and hydrological responses. The first observation that comes from this study is that these three categories of regional groups of homogenous characteristics are sensible with regards to their geographical settings, but the overlap and apparent relationships between them are weak. An explanation of this observation is that there are insufficient data, particularly associated with defining sub-surface processes, and it is possible that additional data would have assisted in the discrimination of more homogenous groups and better links between the different datasets. The model application in this study consisted of two phases: model calibration, using a manual approach, and the application of a physically-based a priori parameter estimation approach. While the first approach was designed to assess the general applicability of the model and identify major errors with regard to input data and model structure, the second approach aimed to establish an understanding of the processes and identify useful relationships between the model parameters and the variations in real hydrological processes. The second approach was also designed to quantify the sensitivity of the model outputs to the parameters of the model and to encompass information sharing between the basin physical characteristics and quantifying the parameters of the model. Collectively, the study’s findings show that these two approaches work well and are appropriate to represent the real hydrological processes of Congo Basin. The secondary objective of this study was achieved by forcing the hydrological model developed for the Congo Basin with downscaled Global Climate Model (GCMs) data in order to assess scenarios of change and future possible impacts on water resources availability within the basin. The results provide useful lessons in terms of basin-wide adaptation measures to future climates. The lessons suggest that there is a risk of developing inappropriate adaptation measures to future climate change based on large scale hydrological response, as the response at small scales shows a completely different picture from that which is based on large scale predictions. While the study has concluded that the application of the hydrological model has been successful and can be used with some degree of confidence for enhanced decision making, there remain a number of uncertainties and opportunities to improve the methods used for water resources assessment within the basin. The focus of future activities from the perspective of practical application should be on improved access to data collection to increase confidence in model predictions, on dissemination of the knowledge generated by this study, and on training in the use of the developed water resources assessment techniques.
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- Date Issued: 2012
Identification of novel marine algal compounds with differential anti-cancer activity: towards a cancer stem-cell specific chemotherapy
- Authors: De la Mare, Jo-Anne
- Date: 2012
- Subjects: Breast -- Cancer , Stem cells -- Research , Chemotherapy , Algae -- Biotechnology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4143 , http://hdl.handle.net/10962/d1016250
- Description: Breast cancer remains the leading cause of cancer-related death in women worldwide. Furthermore, it has been demonstrated that the treatment-resistant ER-PR-HER2/neu- sub-type is more common among women of African descent, necessitating the search for novel chemotherapies for this form of the disease. The secondary metabolites produced by marine algae represent a rich source of structurally unique compounds with chemotherapeutic potential, particularly in South Africa, whose oceans allegedly host 15 % of the total number of species in the world. Indeed, a recent study reported the isolation of a range of novel compounds from South African red and brown algae of the Plocamium, Portiera and Sargassum genera which displayed cytotoxicity against oesophageal cancer cells in vitro. The molecular mechanisms mediating this toxicity were unknown, as was the effect of these and similar compounds on metastatic ER-PR-HER2/neu- breast cancer cell lines or breast cancer stem cells. The current study aimed to address these questions by screening a library of twenty-two novel marine algal compounds for the ability to inhibit MDA-MB-231 and Hs578T breast cancer cells, while having no adverse effects on non-cancerous MCF12A breast epithelial cells. While twelve of these were toxic in the micromolar range against breast cancer cells, only the polyhalogenated monoterpenes RU004 and RU007, and the tetraprenylated quinone sargaquinoic acid (SQA) were identified as hit compounds based on the criteria that their cytotoxicity was specific to breast cancer and not healthy breast cells in vitro. On the other hand, the halogenated monoterpene RU015 was found to be highly toxic to both breast cancer and non-cancerous breast cell lines, while the halogenated monoterpene stereoisomers RU017 and RU018 were non-toxic to either of these cell lines. The mode of action of RU004, RU007, RU015 and SQA, together with the previously characterized carotenoid fucoxanthin (FXN), was assessed in terms of the type of cell death induced and the effect on cell cycle distribution of these compounds. Flow cytometric analysis of the extent of Hoescht 33342 and propidium iodide staining along with PARP cleavage studies suggested that SQA induced apoptosis in MDA-MB-231 cells. On the other hand, the highly toxic compound RU015 appeared to induce necrosis as evidenced by 50 kDa PARP cleavage product in MDA-MB-231 cells. The flow cytometry profiles of MDA-MB-231 and Hst578T cells treated with the hit compounds RU004 and RU007 were suggestive of the induction of apoptosis by these compounds. Cell cycle analysis by flow cytometry with propidium iodide staining revealed that both SQA and FXN induced G0-G1 arrest together with an increase in the apoptotic sub-G0 population, which agreed with previous reports in the literature. The molecular mechanism of action of SQA and FXN were further investigated by the identification of specific signal transducer molecules involved in mediating their anti-cancer activities. SQA was found to require the activity of numerous caspases, including caspase-3, -6, -8, -9, -10 and -13, for its cytotoxicity and was demonstrated to decrease the level of the antiapoptotic protein Bcl-2. On the other hand, FXN was shown to require caspase-1, -2, -3, -9 and - 10 for its toxicity. This, together with the ability to decrease the levels of Bcl-2, pointed to the involvement of the intrinsic pathway in particular in mediating the activity of FXN. The screening of algal compounds against non-cancerous breast epithelial cells carried out in this study, together with the investigation into their mechanisms of action, represent one of the few reports in which characterization of algal metabolites goes beyond the initial cytotoxicity assays. Finally, in order to assess the potential anti-cancer stem cell activity of the marine algal compounds, a subset of these was screened using a mammosphere assay technique developed in this study. The cancer stem cell (CSC) theory proposes that cancers arise from and are maintained by a specific subpopulation of cells able to undergo asymmetric cell division and termed CSCs. These CSCs are capable of anchorage-independent growth in serum-free culture conditions, such as those in the mammosphere assay. Using this assay, the novel halogenated monoterpene stereoisomers RU017 and RU018 were demonstrated to possess putative anti- CSC activity as evidenced by their ability to completely eliminate mammosphere formation in vitro. Furthermore, since RU017 and RU018 were non-toxic to both breast cancer and healthy breast cells, it appeared that the activity of the compounds was potentially specific to the CSCs. The results require further validation, but represent the first report of selective anti-CSC activity.
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- Date Issued: 2012
Identifying expansive learning opportunities to foster a more sustainable food economy: a case study of Rhodes University dining halls
- Authors: Agbedahin, Adesuwa Vanessa
- Date: 2012
- Subjects: Food supply -- Education (Higher) -- South Africa -- Case studies Food industry and trade -- Education (Higher) -- Standards -- South Africa -- Case studies Food service employees -- Education (Higher) -- South Africa -- Case studies
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1560 , http://hdl.handle.net/10962/d1003442
- Description: This is a one year half thesis. This research was conducted within the context of the food services sector of Higher Education Institution dining halls and in the midst of the rising global call for food resource management and food waste reduction. The main aim of this research therefore was to identify opportunities for learning and change for a more sustainable food economy, contributing to Education for Sustainable Production and Consumption, and by further implication, Education for Sustainable Development. To achieve this aim, I used Cultural Historical Activity Theory as theoretical and methodological framework; drawing on the second and the third generations of this theory. Implicated in the above research approach is the identification of expansive learning opportunities from the surfacing of ‘tensions’ and ‘contradictions’. In this case study of the Rhodes University Campus Food Services, such tensions and contradictions inhibiting a more sustainable food economy, involving food waste production were identified. To narrow the scope of the study, one dining hall formed the focus of the case, with a two phased research approach whereby one research question and three goals were developed for each phase. The former being the exploration phase and the latter being the initial stages of the expansive phase. Methods used in line with the methodological framework included ten individual interviews with food producers (staff members), nine focus group discussions with food consumers (students), observations of the dining hall activities which lasted for over a month and two ‘Change Laboratory Workshops’. Some of the findings of this research are that food wastage cannot be addressed and appropriately curtailed without an intensive consideration of all the stages of food economy. Multiple contradictions and sources of tensions embedded in the Food Services Sector constituted major causes of food waste. Additionally, the lack of substantial food waste related teaching and learning activities, the presence of disputed rules, institutional structure and traditional practices within the Food Services all exacerbated the tensions and contradictions. More so, prioritizing some of this identified contradictions and tensions hindering a more sustainable food economy and relegating some as unimportant or nonurgent is unproductive. Finally, the non-existence of facilitated deliberation, consultation, dialogue, collaboration between food producers and food consumers has been identified as an obstacle to learning and institutional change. Recommendations abound in re-orienting, re-educating, and re-informing the constituents of the food economy. Re-visiting and revising of rules and regulations guiding conduct of students and kitchen staff members in the RU dining halls, as well as revision of existing learning support materials and mediating tools in use is needed. Recognition and consideration of the concerns and interests of students and kitchen staff members are also needed. Finally, there is a need to continue to address the tensions and contradictions identified in this case study, to further the Expansive Learning Process if a more sustainable food economy at Rhodes University is to be established.
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- Date Issued: 2012
Identifying volatile emissions associated with False Codling Moth infested citrus fruit
- Authors: Van der Walt, Rachel
- Date: 2012
- Subjects: Citrus -- Diseases and pests , Insect pests , Cryptophlebia leucotreta
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10343 , http://hdl.handle.net/10948/d1020056
- Description: False codling moth is a known pest of economic importance to many cultivated crops in South Africa and Africa south of the Sahara, and is particularly severe on citrus. If the fruit is infested just before harvest the chances of detecting signs of infestation are very low. As a result, the risk of packaging infested fruit and exporting them as healthy fruit is high. It is therefore a priority to develop a post-harvest technique for detection of False codling moth in citrus fruit at different levels of infestation in order to reduce phytosanitary risk. Compounds released and detected were indicative of infestation and were not insect produced but naturally produced fruit volatiles emitted at higher levels as a result of the insect within the fruit. Five major volatile compounds of interest were released by the infested oranges. These major volatile compounds include D-limonene, 3,7-dimethyl-1,3,6-octatriene, (E)-4,8-dimethyl-1,3,7-nonatriene, caryophyllene and naphthalene. Limonene was one of the most abundant volatile compounds released by the infested citrus fruit. Naphthalene, which is possibly produced due to larval feeding and development within the fruit maintained higher concentrations than controls throughout the infestation within the fruit. Naphthalene would be a good indicator of False codling moth infestation, however, not primarily for early infestation detection. A significantly higher concentration of D-limonene, 3,7-dimethyl-1,3,6-octatriene, (E)-4,8-dimethyl-1,3,7-nonatriene and naphthalene was detected using the SEP over the SPME technique. The application of an SPME procedure and the utilization of this method for detection of volatiles present in the headspace of intact infested fruit are evaluated and the possible volatile compounds diagnostic of Thaumatotibia leucotreta infestation of orange fruit and differences in volatile compound response in different orange varieties is discussed.
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- Date Issued: 2012
Illustrated medicines information for HIV/AIDS patients: influence on adherence,self-efficacy and health outcomes
- Authors: Barford, Kirsty-Lee
- Date: 2012
- Subjects: AIDS (Disease) -- Treatment -- South Africa , HIV infections -- Treatment -- South Africa , AIDS (Disease) -- Patients -- South Africa , HIV-positive persons -- South Africa , AIDS (Disease) -- Study and teaching -- South Africa , Antiretroviral agents -- South Africa , Communication in medicine -- South Africa , Communication in public health -- South Africa
- Language: English
- Type: Thesis , Masters , MPharm
- Identifier: vital:3863 , http://hdl.handle.net/10962/d1015678
- Description: South Africa has an estimated 920 000 patients on antiretrovirals (ARVs), the largest number of patients in any country. ARV therapy demands adherence levels in excess of 95% to avoid development of drug resistance, but adherence to ARV therapy is estimated to be only between 50% and 70%. Poor medication adherence is acknowledged as a major public health problem, reducing the effectiveness of therapy and promoting resistance to ARVs. More than two thirds of the South African population have marginal reading skills and this significantly influences a patient’s ability to read and understand health-related information. Patient education materials tailored for the South African population could be a useful aid in facilitating communication with patients and perhaps impact positively on their medicine-taking behaviour. This behaviour is influenced by patient knowledge, beliefs, attitudes and expectations and includes self-management, self-efficacy and adherence. Self-efficacy, which refers to patient confidence in the ability to self-manage medicine taking, is a key factor influencing adherence. This study aimed to develop illustrated patient information leaflets (PILs) and medicine labels for all first-line ARV regimens used in the public health sector in South Africa and, using a randomised control study design, to investigate the impact of these illustrated information materials on knowledge, medication-taking behaviours and health outcomes in HIV/AIDS patients taking ARVs. To achieve this aim, the objectives were to assess HIV/AIDS and ARV-related knowledge, as well as self-efficacy and adherence to ARV therapy; to assess the influence of demographic variables on knowledge, adherence and self-efficacy; to assess the influence of the information materials on knowledge, self-efficacy and adherence and to assess the association of knowledge with health outcomes. Medicine labels and PILs, both English and isiXhosa, were developed for ARV regimens 1a, 1b, 1c and 1d. The 8-item Morisky Medication Adherence Scale (MMAS-8) and HIV Treatment Adherence Self Efficacy Scale (HIV-ASES) instruments for measuring respectively adherence and self-efficacy, were modified to optimize clarity, simplicity and cultural acceptability and were translated into isiXhosa using a multi-stage translation-back translation. The questions and the rating scales, for both the MMAS and HIV-ASES, underwent preliminary qualitative evaluation in focus group discussions. Patients were recruited from local Grahamstown clinics. A pilot study to evaluate applicability of the instruments was conducted in 16 isiXhosa AIDS patients on ARVs and the results from this study informed further modifications to the instruments. One hundred and seventeen patients were recruited for the randomised control trial and were randomly allocated to either control group (who received standard care) or experimental group (who received standard care as well as pictogram medicine labels and the illustrated PIL). Interviews were conducted at baseline and at one, three and six months. Data were analysed statistically using the t-test, chi-squared test and ANOVA (Analysis of Variance) at a 5% level of significance. Correlations were determined using Pearson and Spearman rho correlations. Approval was obtained from Rhodes University Ethical Standards Committee, Settlers Hospital Ethics Committee and the Eastern Cape Department of Health. The results of this research showed that illustrated PILs and medicine labels enhanced understanding of HIV/AIDS and ARV information, resulting in a mean overall knowledge score in the experimental group of 96%, which was significantly higher than the 75% measured in the control group. Variable knowledge scores were measured in three areas: baseline knowledge of general HIV/AIDS-related information was good at 87%, whereas knowledge scores relating to ARV-related information (60%) and side-effects (52%) were lower. These scores improved significantly in the experimental group over the 4 interviews during the 6 month trial duration, whereas in the control group, they fluctuated only slightly around the original baseline score. There was no significant influence of gender on knowledge score, whereas health literacy, education level and age tested (at one and three months) had a significant influence on knowledge. Self-efficacy and adherence results were high, indicating that the patients have confidence in their ability to adhere to the ARV therapy and to practice optimal self-care. Age, gender and education, in most cases, significantly influenced self-efficacy, but were found to have no effect on adherence. The CD4 count improved over the trial duration which may have been influenced by a number of factors, including better knowledge of ARVs and improved adherence. No significant parametric correlation was found between knowledge score and change in CD4 count, however, Spearman's rho showed significance (rs=0.498; p=0.022). Both patients and healthcare providers were highly enthusiastic about the illustrated labels and PILs, and indicated their desire for such materials to be routinely available to public sector HIV/AIDS patients. The isiXhosa version of the PIL was preferred by all the patients. These simple, easy-to-read leaflets and illustrated medication labels were shown to increase understanding and knowledge of ARVs and HIV/AIDS in low-literate patients, and their availability in the first-language of the patients was central to making them a highly useful information source.
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- Date Issued: 2012
Imidazole-functionalized polymer microspheres and fibers–useful materials for immobilization of oxovanadium (IV) catalysts
- Authors: Walmsley, Ryan S , Ogunlaja, Adeniyi S , Coombes, Matthew J , Chidawanyika, Wadzanai J U , Litwinski, Christian , Torto, Nelson , Nyokong, Tebello , Tshentu, Zenixole R
- Date: 2012
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/246041 , vital:51431 , xlink:href="https://doi.org/10.1039/C2JM15485D"
- Description: Both polymer microspheres and microfibers containing the imidazole functionality have been prepared and used to immobilize oxovanadium(IV). The average diameters and BET surface areas of the microspheres were 322 μm and 155 m2 g−1 while the fibers were 1.85 μm and 52 m2 g−1, respectively. XPS and microanalysis confirmed the incorporation of imidazole and vanadium in the polymeric materials. The catalytic activity of both materials was evaluated using the hydrogen peroxide facilitated oxidation of thioanisole. The microspheres were applied in a typical laboratory batch reactor set-up and quantitative conversions (>99%) were obtained in under 240 min with turn-over frequencies ranging from 21.89 to 265.53 h−1, depending on the quantity of catalyst and temperature. The microspherical catalysts also proved to be recyclable with no drop in activity being observed after three successive reactions. The vanadium functionalized fibers were applied in a pseudo continuous flow set-up. Factors influencing the overall conversion and product selectivity, including flow rate and catalyst quantity, were investigated. At flow rates of 1–4 mL h−1 near quantitative conversion was maintained over an extended period. Keeping the mass of catalyst constant (0.025 g) and varying the flow rate from 1–6 mL h−1 resulted in a shift in the formation of the oxidation product methyl phenyl sulfone from 60.1 to 18.6%.
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- Date Issued: 2012
Impact of animal traction power on agricultural productivity: case of lowlands of Mohale's Hoek district of Lesotho
- Authors: Rampokanyo, Lepolesa Michael
- Date: 2012
- Subjects: Animal traction -- Lesotho , Agricultural productivity -- Lesotho , Draft animals -- Lesotho , Food security -- Lesotho , Farm produce -- Lesotho
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11178 , http://hdl.handle.net/10353/483 , Animal traction -- Lesotho , Agricultural productivity -- Lesotho , Draft animals -- Lesotho , Food security -- Lesotho , Farm produce -- Lesotho
- Description: Most farming at subsistence level is located in rural areas where the majority of smallholder farmers have low productivity which results in high rate of food insecurity. The areas are characterised by animal traction and poor farming practises, and monoculture is mostly preferred. In light of this, this study analyzed the impact of animal power on agricultural productivity. Smallholder farmers in the lowlands of Mohale’s Hoek district of Lesotho were investigated by means of a case study methodology. The aim of the study was to inform agricultural policy about the level and key determinants of inefficiency in the smallholder farming system so as to contribute to policy designed to raise productivity of smallholder farmers. The sampling frame comprised farmers and extension workers in the lowlands of Mohale’s Hoek district. From this frame, 118 farmers and 4 extension workers were randomly selected from four villages, namely‘Mapotsane, Potsane, Tsoloane and Siloe. The four groups of farmers include; the farmers owning and using cattle for ploughing, farmers owning tractor and cattle and using them for ploughing, farmers owning tractor only and using it for ploughing, and farmers who owned neither cattle nor tractor and normally hire these when ploughing operations are to be done on the farm. The interviews of these farmers and extension workers were conducted by means of semi-structured questionnaire which consisted of both open and close ended questions. The study used the stochastic frontier production model for the production efficiencies and linear regression model for the impact of animal traction on agricultural productivity. Both procedures provided insights into the relative contributions of animal power and traditional systems to poverty alleviation and food security in the project areas. Descriptive statistics were employed for farming systems and challenges facing small scale farmers. Gross Margins analysis was conducted for the animal power and tractor power yield levels for maize crop to compare the two types of power. Some diagnostic tests to detect serial correlation and heteroskedasticity and t-tests were also performed. The significant variables include the area of sorghum ploughed, members of the household that assist with family labour, education, quantity of fertilizer applied, time taken by the farmers in farming, members who are formally employed, household size, area of land ploughed, old age, costs of tractor and animal, marital status, income, area of maize ploughed, area of sorghum ploughed, quantity of fertilizers applied, costs of seeds and fertilizers applied, maize and sorghum yield and amount sold and consumed. The study revealed that monoculture is mainly practised and many smallholder farmers used traditional technologies that fail to replace nutrients in the soil. Nonetheless family labour was not a problem. During the farming season, tractors were used as the main source of power for ploughing. Most farmers hired these for maize production as it is a staple food crop even where animal power is available. It was noted that the tractors were few and in most cases old and malfunctioning. The cost of using animals in farming obviouslyplayed a role in the production of both maize v and sorghum in the lowland areas of Mohale’s Hoek district. Smallholder farmers who owned both tractors and animals produced more but they were mainly affected by high costs of maintaining the aging tractors, generally purchased on the used-equipment market. The increased challenges resulted in lower productivity of the smallholder farmers, including: unhealthy animals, drought, marketing problems, late ploughing, poor soils, lack of extension services, low yields, low income, lack of information, lack of appropriate implements, lack of support services, nutrition inadequacy, inappropriate farming systems. The study recommended the adoption and promotion of low-cost mechanization in the lowlands of Mohale’s Hoek district so as to increase the production of the smallholder farmers. Increased productivity will in turn improve household food security.
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- Date Issued: 2012
Impact of economic freedom on CEMAC countries
- Authors: Ossono NII, Edith Gloria
- Date: 2012
- Subjects: Free enterprise , Economic development , Monetary policy -- Africa, Central , Africa, Central -- Economic integration , Africa, Central -- Economic policy
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:9020 , http://hdl.handle.net/10948/d1019713
- Description: The study aimed to evaluate the impact of economic freedom on economic growth and investments in the Economic and Monetary Community of Central Africa (CEMAC). The region was created in 1994 by the six states of Cameroon, Chad, the Central African Republic, the Republic of Congo, Gabon and Equatorial Guinea. CEMAC countries comprise low and middle-income countries that share the same currency - the CFA Franc. The CEMAC countries were observed between 1995 and 2008 and panel regression methodologies were employed. A positive impact of economic freedom on economic growth was established using fixed effects method and the generalised method of moments. The impact of a unit increase in the economic freedom index on GDP per capita ranged between 72.65 and 124.51 units (dollars) increase on GDP per capita, ceteris paribus. Economic freedom was also found to Granger-cause economic growth. The results underline a significantly positive relationship between economic freedom and economic growth which is consistent with existing literature. The impact of economic freedom on domestic investment and foreign directs investment was then examined. With regard to domestic investment, economic freedom was found to be statistically significant and positive in all specifications of the model, thereby implying that a unit increase in the economic freedom index increases domestic investment by values of between 0.50 and 0.69 dollars in the CEMAC. The results obtained were consistent with most findings on the relationship between economic freedom and investments. With regard to the relationship between economic freedom and foreign direct investment inflows, economic freedom was unexpectedly statistically insignificant in most specifications of the model. The latter implies that economic freedom does not have a significant impact on foreign direct investment in the CEMAC. However, the study revealed that economic freedom Granger-causes foreign direct investment but foreign direct investment does not Granger-cause economic freedom. This means that economic freedom precedes foreign direct investments, and foreign direct investments do not precede economic freedom. The study strongly recommends an improvement of institutions in the CEMAC in order to enjoy greater levels of economic freedom and therefore foster economic growth and domestic investment in the region.
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- Date Issued: 2012