Assessing invasive alien aquatic plant species, phytoremediation effects using biological indicators in the Swartkops River system
- Authors: Tshithukhe, Getrude
- Date: 2020
- Subjects: Aquatic weeds -- Biological control -- South Africa -- Eastern Cape , Alien plants -- Biological control -- South Africa -- Eastern Cape , Phytoremediation -- South Africa
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167665 , vital:41501
- Description: Pollution effluents in freshwater ecosystems are becoming increasingly ubiquitous as a result of cumulative anthropogenic activities, such as wastewater treatments works, and industrial, agricultural and mining activities. These activities are more noticeable in urban river atchments where there is greater human population densities and industrial developments. The ecological effects of anthropogenic activities on freshwater ecosystems include: excessive deposition of contaminants such as nutrients, pharmaceuticals, microplastics and other chemicals, which change physicochemical properties, causing a decline in aquatic biodiversity. These effects, impact negatively on the resilience of freshwater, making the systems vulnerable to invasion by alien aquatic plants. Ultimately, the loss of local biodiversity associated with the invasive alien aquatic plants (IAAP) results in a loss of some ecosystem goods and services. The Swartkops River system, Eastern Cape Province, drains most of the neighbouring formal and informal settlements, agricultural lands and industries, and hence is exposed to water pollution from human activities along the river catchment. Various water quality assessments are needed to evaluate the extent of pollutants and their impacts on the river ecosystem. Phytoremediation is one approach employed internationally for removing harmful nutrients and chemicals in freshwater ecosystems. Most studies measure the success of phytoremediation through measuring the reduction of contaminants in water or soil chemistry in mesocosm settings, which may not take into account all the important environmental factors that exist in the field. The present study assesses the phytoremediation potential of Pontederia (Eichhornia) crassipes and Salvinia molesta by evaluating water and sediment chemistry, periphyton and aquatic macroinvertebrate community recovery along seven field sites (excluding IAAP species mats sites) located upstream and downstream IAAP species mats on the Swartkops River between April and September 2018. Water and sediment samples were collected once monthly on ten seven field sites, including the IAAP species mat sites. Periphyton and aquatic macroinvertebrates were collected on seven sites, excluding the IAAP mat sites. Pontederia crassipes and S. molesta infestation in Swartkops River showed positive phytoremediation results and improved some water and sediment chemistry in the downstream treatments as compared to upstream treatments. Although there were some fluctuations with some variables, important water and sediment chemistry were reduced downstream. By contrast, biological assessment results did not show any response to the presence of IAAP species and phytoremediation. Periphyton and aquatic macroinvertebrates diversity and community assemblages were more influenced by water quality. Although IAAP species did provide improvement in water and sediments chemistry, multiple effluent point and non-point sources in Swartkops outpaced phytoremediation. Taxa evenness and relative taxa abundance showed significant differences between the upstream and downstream sites, however taxa richness and Shannon’s diversity showed no significant differences, indicating no relative recovery in biodiversity for either periphyton or aquatic macroinvertebrates. Similarly, the upstream and downstream sites showed similar periphyton and aquatic macroinvertebrates assemblages structure, all dominated by pollution tolerant taxa, thus indicating no functional diversity improvement down river; because of improvement in water chemistry downstream sites, it was expected that periphyton and aquatic macroinvertebrates assemblage structure would also improve at these downstream sites.It is possible that the phytoremediation process is outpaced by effluent discharges, given the multiple sources and distance between upstream and downstream mat sites. The study showed phytoremediation potential but the results were not indicated by biological indicators. A replica study conducted on a moderately disturbed river system is recommended to measure the success and recovery of biological indicators and assemblage composition following IAAP species phytoremediation; a moderately disturbed river compared to a largely disturbed river will broaden findings and look at differences for a wider application of phytoremediation.
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- Date Issued: 2020
Initiating biological control for Nymphaea mexicana zuccarini (Nymphaeaceae) in South Africa
- Authors: Reid, Megan Kim
- Date: 2020
- Subjects: Nymphaea mexicana zuccarini -- Biological control -- South Africa , Nymphaeaceae -- Biological control -- South Africa , Invasive plants -- Biological control -- South Africa
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/144510 , vital:38352
- Description: Nymphaea mexicana Zuccarini (Nymphaeaceae) is an aquatic plant originating from south-eastern USA that is becoming increasingly invasive in South Africa as other invasive aquatic plants are being managed successfully through biological control. Mechanical and chemical control of aquatic weeds is expensive, damaging to the environment, and only effective in the short term, so biological control is more desirable as a management strategy for N. mexicana. The biological control of invasive alien plants requires that agents are host specific so that non-target risks are mitigated. For success to be achieved, it is important to ensure that the genetic structure of invasive populations is clarified so that agents can be collected from populations in the native range that match genetically to populations in the invasive range. This is especially important in cases where the morphology of invasive alien plants does not reflect genetic differences between populations. A previous study of the genetic structure of the invasive populations of N. mexicana in South Africa suggests the presence of hybrid forms of the plant in South Africa, with only one of these populations matching with samples from the native range. However, the study only used samples from two sites in the native range using amplified fragment length polymorphisms (AFLPs), so it was necessary to conduct further genetic analyses using samples from more sites in the native range. Hence, the first aim of this study was to develop a better understanding of the genetic structure of N. mexicana populations in the native and invaded range. Genetic samples were collected from sites in the native range during field surveys for potential biological control agents, and inter-simple sequence repeats (ISSRs) were used to compare the genetic structure of invasive and native populations of N. mexicana in South Africa. The results from these analyses suggest that seven of the 14 investigated invasive populations of N. mexicana in South Africa are genetically similar to populations in the native range, while the remaining seven populations are likely to be hybrid forms of the plant. This knowledge will be useful to target populations for biological control and highlights the need for further genetic analyses to determine the parentage of these hybrids so that biological control efforts are more likely to be successful. The initiation of a biological control programme requires that a series of steps are taken in order to maximise the likelihood that this form of intervention will be successful. The first few steps include: identification of the target weed and its genetic structure; exploration in the native range for potential biological control agents; and prioritisation of these agents based on factors such as climatic and genetic compatibility, feeding damage, abundance, and likely host range. Hence, the second aim of this study was to conduct surveys for potential biological control agents in the native range of N. mexicana, and to prioritise these agents. Field surveys were conducted between August and October in 2018 at 17 sites in Florida, Louisiana, and Texas, USA. Sites were selected based on climatic similarity of native sites compared to invasive sites by use of MaxEnt modelling. Native N. mexicana plants were searched for natural enemies, and these were prioritised based on feeding damage, abundance, incidence, and observations of field host range. Two species were prioritised: Bagous americanus LeConte (Coleoptera: Curculionidae) and Megamelus toddi Beamer (Hemiptera: Delphacidae). These species will be imported into quarantine facilities at Rhodes University for host specificity tests to be conducted. Understanding the factors that contribute to the successful establishment of biological control agents is important to improve the efficiency and reduce the costs incurred during the initiation of biological control programmes. Acquiring knowledge of the factors that predict the efficacy of biological control agents is similarly important, and these factors are discussed in the last chapter of this study. The challenges of the biological control of hybrids are also considered, and recommendations are made for the control of N. mexicana and other plants in South Africa.
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- Date Issued: 2020
Post release evaluation of the distribution and efficacy of Eccritotarsus catarinensis and Eccritotarsus eichhorniae on Pontederia crassipes in South Africa
- Authors: Maseko, Zolile
- Date: 2020
- Subjects: Water hyacinth -- Biological control -- South Africa , Weeds -- Biological control -- South Africa , Miridae -- South Africa , Insects as biological pest control agents -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/143046 , vital:38196
- Description: Biological control involves the release of new species into the environment and therefore, needs to be carefully monitored through post-release assessments which have been largely neglected in the science. Post-release evaluations of biological control programmes reveal whether the control agent has established and if it impacts weed demography, while cost-benefit analyses require a different set of data that show the magnitude on return on investment. The biological control effort on Pontederia crassipes in South Africa uses, amongst others, two species of mirid, Eccritotarsus catarinensis and E. eichhorniae. Initially, they were released as a single species, but were recently divided using molecular techniques. Eccritotarsus catarinensis was released in 1999, and E. eichhorniae in 2007. After many releases over two decades, there was need to assess where each species was established in the country. Molecular techniques proved to be valuable in identifying the two species as they are morphologically indistinguishable in the field. Therefore, molecular techniques should be routinely used for screening biocontrol agents, whether new or as re-introductions. Annual surveys of the mirid release sites around South Africa were undertaken between 2016 and 2019. At each site both insect and plant parameters were measured. Only E. catarinensis is established in the field in South Africa despite the multiple releases of E. eichhorniae at over 70 sites across the country, and E. catarinensis has established at only 22 of the 45 release sites accessed during this study. This thesis tested climate, interaction with other agents already on P. crassipes, and direct competition between the two mirid species as reasons for the lack of establishment of E. eichhorniae. The results of the country-wide surveys showed that climate and water trophic status were the major determinants in the establishment of E. catarinensis. Most of the establishment was recorded in the warmer regions of the country, however, a few populations of the mirid also established in cooler areas, thus demonstrating a degree of thermal plasticity, and possible microclimates as the mirids persisted at sites shaded by riparian vegetation. Stochastic events such as active herbicide campaigns, winter frosts, droughts and floods were responsible for the absence of the mirid at some sites. At some of the eutrophic sites, despite the abundance of E. catarinensis, plants still proliferated as the water trophic status facilitated plant growth, thus, plants were able to compensate for the damage inflicted by the mirid. A more intensive, monthly, post-release evaluation was conducted on the Kubusi River, Eastern Cape Province between 2016 and 2019. This is regarded as one of the cooler water hyacinth sites. Populations of biological control agents at this site fluctuated seasonally. At this site, cold winters caused frosting of the leaves of P. crassipes with the exception of plants growing under overhanging vegetation that provided a refuge for the mirid. But, cool temperatures in the winter months (May to August) severely reduced the populations of E. catarinensis that required a long recovery phase in spring. The consequence of this was that the plants grew unchecked from the onset of the growing season forming dense mats. Of the four agents at the Kubusi River site, Eccritotarsus catarinensis recovered slowest after winter, with lag phases ranging from two months to several months of the three-year period. The release of a suite of agents has implications on the agents themselves, where interactions between the agents can be important. Interactions between pairs and even multiple agents can have implications for biocontrol, where agents are either complimentary or interfere with each other. In this case, because E. catarinensis recovered the slowest of the four agents at the site, plants were of a poor quality by mid-summer resulting in low mirid populations. Competition in weed biological control could be expected to be strongest between pairs of agents that share the same niche, and this could be the reason why E. eichhorniae failed to establish at sites where E. catarinensis had already been established for several years. When the two mirids were combined in manipulated trials in a polytunnel, populations were lower compared to when the two mirids occurred separately. Under warm conditions, it is likely that E. eichhorniae would be the superior agent compared to E. catarinensis. The evaluations discussed in this thesis highlighted gaps in agent release methodology in multispecies settings, as well as the need for strategic augmentation pre- and post-winter. It is important to release agents that will complement each other rather than compete, therefore, when releasing agents in a multispecies setting, niche differentiation needs to be considered. Here it is concluded that the best practice for dealing with the mirids is that they should be released individually, and at sites that have no other biological control agents in order to ultimately assess their efficacy. Landscape level, long-term monitoring of biological control programmes shows the impact of the control programme at a broader scale and, are far more informative than short-term studies and at fewer sites. Long-term post-release evaluations should be mandatory in biological control programmes. Furthermore, these assessments will help develop new strategies or improve on existing ones, thus achieve greater success in control.
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- Date Issued: 2020
Sexual attraction and mating compatibility between Thaumatotibia leucotreta populations and implications for semiochemical dependent technologies
- Authors: Upfold, Jennifer Kate
- Date: 2020
- Subjects: Cryptophlebia leucotreta -- South Africa , Cryptophlebia leucotreta -- Reproduction , Citrus -- Disease and pests -- Control -- South Africa , Insect sterilization -- South Africa , Pheromones , Pheromone traps
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/148526 , vital:38747
- Description: False codling moth (FCM), Thaumatotibia leucotreta (Meyrick), is the most important pest for the cultivation of citrus in South Africa. False codling moth is indigenous to southern Africa and is a regulated pest of many international markets for phytosanitary concerns. Considerable research efforts have been invested in the past decades to develop semiochemcial technologies, such as monitoring with sex pheromones, attract-and-kill, mating disruption and the sterile insect technique. One of the potential obstacles identified with semiochemical control is the differences in the ratio of the compounds comprising the sex pheromone at different geographical locations, resulting in what is known as regional attraction. This has been identified in FCM populations from three different countries, however, regional attraction within South African FCM populations was unknown. Therefore, the study assessed the genetic integrity of five laboratory-reared FCM populations originating from geographically isolated populations in South Africa using the AFLP technique in order to assess regional attractiveness within the country. The results found isolated populations from Addo, Citrusdal, Marble Hall, Nelspruit and a fifth group found to be closely related to Addo and Citrusdal called the ‘Old’ colony. These five genetically isolated populations as well as a population from Xsit (Pty) Ltd, used for the sterile insect technique (SIT), were used in regional attractiveness trials. Males were significantly (P = <0.05) more attracted to females originating from the same population. No significant attraction could be determined from the sterile males, as the recapture rates in the trap were too low. Furthermore, regional attractiveness was assessed through choice/ no-choice mating compatibility trials. Significant sexual isolation (ISI) occurred between mating combinations Addo × Nelspruit (ISI = 0,13; t2 = 6.23; p = 0.02), Addo × Marble Hall (ISI = 0,11; t2 = 4.72; p = 0.04), Citrusdal × Nelspruit (ISI = 0,11; t2 = 4.95; p = 0.04), and Citrusdal × Marble Hall (ISI = 0,12; t2 = 4.31; p = 0.04). In these combinations, Addo and Citrusdal males were found to have outcompeted Nelspruit and Marble Hall males for more mating events. Significant sexual isolation was also recorded for Sterile × Marble Hall (ISI = 0.12; t2 = 4.98; p =0.01) and Sterile × Citrusdal (ISI = 0.13; t2 = 3.96; p = 0.01) populations. The male relative performance index was significant in both combinations, indicating that non-sterile laboratory males outcompeted the sterile males in these two combinations. When given no choice, evaluated as spermatophore transfer/ female/ 48h, all males (including sterile) were successful in transferring spermatophores to all FCM populations, with no significant differences. These results indicate that there may be incipient pre-isolation mechanisms affected by local natural selection, resulting in localised sexual attraction via differences in the sex pheromone ratios. These findings provide important information for semiochemical technologies and the implication of these results with regard to monitoring with sex pheromones, attract-and-kill, mating disruption and sterile insect technique are discussed.
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- Date Issued: 2020
The effects of elevated atmospheric CO2 on the biological control of invasive aquatic weeds in South Africa
- Authors: Baso, Nompumelelo Catherine
- Date: 2020
- Subjects: Aquatic weeds -- Biological control -- South Africa , Plants -- Effect of atmospheric carbon dioxide on , Atmospheric carbon dioxide -- Environmental aspects
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/140772 , vital:37917
- Description: There has been a rapid increase in atmospheric CO2 concentration, from pre-industrial values of 280 ppm to more than 400 ppm currently, and this is expected to more than double by the end of the 21st century. Studies have shown that plants grown above 600 ppm tend to have an increased growth rate and invest more in carbon-based defences. This has important implications for the management of invasive alien plants, especially for the field of biological control which is mostly dependent on herbivorous insects. This is because insects reared on such plants have been shown to have reduced overall fitness. Nevertheless, most of the studies on potential changes in plant-insect interactions under elevated CO2 are based on agricultural systems, with only a limited number of these types of studies conducted on alien invasive weeds. However, climate change and invasive species are two of the most prevalent features of global environmental change. Therefore, this also warrants active research and experimental studies to better understand how these systems will be affected by future climates. Thus, the aim of this study was to investigate the effects of elevated atmospheric CO2 on the biological control of four invasive aquatic weeds (Azolla filiculoides, Salvinia molesta, Pistia stratiotes, and Myriophyllum aquaticum). These species are a threat to natural resources in South Africa but are currently under successful control by their biological control agents (Stenopelmus rufinasus, Cyrtobagous salviniae, Neohydronomus affinis, and Lysathia n. sp.). To achieve this, the selected plant species were grown in a three-factor experimental design in winter (CO2 X nutrients X herbivory), and another two-factorial design in summer (CO2 X herbivory). Atmospheric CO2 concentrations were set at ambient (400 ppm) or elevated (800 ppm), as per the predictions of the IPCC. As per my hypothesis, the results suggest that these species will become more challenging in future due to increased biomass production, asexual reproduction and a higher C: N ratio which is evident under high CO2 concentrations. Although the biological control agents were in some instances able to reduce this CO2 fertilisation effect, their efficacy was significantly reduced compared with the levels of control observed at ambient CO2. These results suggest that additional biological control agents and other management methods may be needed for continued control of these invasive macrophytes, both in South Africa and further afield where they are problematic.
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- Date Issued: 2020