Elucidation of a novel role for HSP70/HSP90 organising protein (Hop) in mRNA processing
- Dingle, Laura Margaret Kirkpatrick
- Authors: Dingle, Laura Margaret Kirkpatrick
- Date: 2020
- Language: English
- Type: thesis , text , Doctoral , Ph.D
- Identifier: http://hdl.handle.net/10962/59173 , vital:27449 , doi:10.21504/10962/59173
- Description: Thesis (PhD.)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020.
- Full Text:
- Date Issued: 2020
- Authors: Dingle, Laura Margaret Kirkpatrick
- Date: 2020
- Language: English
- Type: thesis , text , Doctoral , Ph.D
- Identifier: http://hdl.handle.net/10962/59173 , vital:27449 , doi:10.21504/10962/59173
- Description: Thesis (PhD.)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020.
- Full Text:
- Date Issued: 2020
Elucidation of the effect of cannabinoids on ER stress to determine their molecular mechanism of action in breast cancer cells
- Authors: De la Harpe, Amy
- Date: 2020
- Subjects: Endoplasmic reticulum -- Pathophysiology , Breast -- Cancer -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/48213 , vital:40525
- Description: Endoplasmic reticulum (ER) stress is defined as an imbalance between the ER’s protein-folding load and folding capacity. ER stress is induced by various physiological conditions and subsequently triggers the activation of the unfolded protein response (UPR) to re-establish homeostasis and promote cell survival. However, under severe or chronic stress, apoptosis is induced. Normal cells generally do not experience ER stress; however, stressful conditions in the tumour micro-environment facilitates chronic ER stress and UPR activation, which plays a pivotal role in tumour survival. Exacerbation of pre-existing ER stress can trigger cancer cell death, with a minimal effect on normal cells. Currently, no high-throughput method exists to detect and quantify ER stress in cell lines. This study showed that Thioflavin T, a fluorescent dye that binds to misfolded protein aggregates, can be used for the high-throughput detection of ER stress, and provides several advantages over currently used methods. Current literature suggests that cannabinoid treatment may induce cancer cell death via ER stress, however little is known about the mechanism of induction. This study proposed a mechanism that occurs via the influx of Ca2+ via the vanilloid receptor 1 (TRPV1), and subsequent ROS production, which affects protein folding. ER stress was induced using various cannabinoids and measured using Thioflavin T and western blot analysis. The effect of cannabinoid treatment on ROS production and the intracellular Ca2+ concentration was measured. Cannabidiol (CBD) was the most potent ER stress inducer, significantly increasing Ca2+ and ROS accumulation; however, the level of accumulated Ca2+ across cell lines varied, which may be due to the differences in the TRPV1 expression and localization. Concomitant treatment of CBD with an antioxidant significantly increased cell viability and decreased ER stress induction in the MCF7 cell line. Concomitant treatment with a TRPV1 antagonist increased viability in this cell line. In conclusion, the results suggested that CBD may induce ER stress via Ca2+ influx through the TRPV1 receptor, thereby elevating intracellular ROS levels and disrupting protein folding.
- Full Text:
- Date Issued: 2020
- Authors: De la Harpe, Amy
- Date: 2020
- Subjects: Endoplasmic reticulum -- Pathophysiology , Breast -- Cancer -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/48213 , vital:40525
- Description: Endoplasmic reticulum (ER) stress is defined as an imbalance between the ER’s protein-folding load and folding capacity. ER stress is induced by various physiological conditions and subsequently triggers the activation of the unfolded protein response (UPR) to re-establish homeostasis and promote cell survival. However, under severe or chronic stress, apoptosis is induced. Normal cells generally do not experience ER stress; however, stressful conditions in the tumour micro-environment facilitates chronic ER stress and UPR activation, which plays a pivotal role in tumour survival. Exacerbation of pre-existing ER stress can trigger cancer cell death, with a minimal effect on normal cells. Currently, no high-throughput method exists to detect and quantify ER stress in cell lines. This study showed that Thioflavin T, a fluorescent dye that binds to misfolded protein aggregates, can be used for the high-throughput detection of ER stress, and provides several advantages over currently used methods. Current literature suggests that cannabinoid treatment may induce cancer cell death via ER stress, however little is known about the mechanism of induction. This study proposed a mechanism that occurs via the influx of Ca2+ via the vanilloid receptor 1 (TRPV1), and subsequent ROS production, which affects protein folding. ER stress was induced using various cannabinoids and measured using Thioflavin T and western blot analysis. The effect of cannabinoid treatment on ROS production and the intracellular Ca2+ concentration was measured. Cannabidiol (CBD) was the most potent ER stress inducer, significantly increasing Ca2+ and ROS accumulation; however, the level of accumulated Ca2+ across cell lines varied, which may be due to the differences in the TRPV1 expression and localization. Concomitant treatment of CBD with an antioxidant significantly increased cell viability and decreased ER stress induction in the MCF7 cell line. Concomitant treatment with a TRPV1 antagonist increased viability in this cell line. In conclusion, the results suggested that CBD may induce ER stress via Ca2+ influx through the TRPV1 receptor, thereby elevating intracellular ROS levels and disrupting protein folding.
- Full Text:
- Date Issued: 2020
Employee retention as a strategy for the sustainability of rehabilitation services within the Nelson Mandela Bay Health District
- Authors: Campbell, Teneille Dawn
- Date: 2020
- Subjects: Employee retention--South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/47530 , vital:40124
- Description: Health care facilities in the South African public sector have, over time, become notorious for poor service delivery, peppered with issues of poor governance, weak administration capabilities, and ailing working conditions. Often, the consequence hereof is a loss of good talent to both the private sector and health sectors abroad, all the while placing more strain on institutional service delivery capabilities. To achieve the United Nation’s Sustainable Development Goal (SDG) of Universal Health Coverage (UHC) by 2030, the strengthening of health systems is critical. The purpose of this study is therefore to offer supportable solutions to improve and maintain a sustainable rehabilitation service in the Nelson Mandela Metro through successful retention of staff. The aim of this study then is to investigate the influence that Human Resources (HR) processes, working conditions and leadership have had on retention of staff. A review of the available literature was conducted on possible factors that contribute to the problem and the solutions thereof. The study population included all staff that worked in rehabilitation service departments in the public health institutions in the Nelson Mandela Metro, namely; physiotherapy, occupational therapy, speech therapy and audiology. The sample constituted n=51 respondents. Data was collected via a questionnaire as the selected measurement tool. The data was analysed using the statistical data analysis software system, TIBCO Statistica (version 13), and selected methods for quantitative data. The results of the study showed that only HR processes were significantly and positively (r = 0.67, p < 0.05) related to retention. It means that satisfying the HR needs of this population is most likely to retain their employ and improve and maintain rehabilitation service delivery in the selected metro. Descriptive statistics of all the variables were also calculated and reported on. Lastly, the managerial implications of the study findings were reported on.
- Full Text:
- Date Issued: 2020
- Authors: Campbell, Teneille Dawn
- Date: 2020
- Subjects: Employee retention--South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/47530 , vital:40124
- Description: Health care facilities in the South African public sector have, over time, become notorious for poor service delivery, peppered with issues of poor governance, weak administration capabilities, and ailing working conditions. Often, the consequence hereof is a loss of good talent to both the private sector and health sectors abroad, all the while placing more strain on institutional service delivery capabilities. To achieve the United Nation’s Sustainable Development Goal (SDG) of Universal Health Coverage (UHC) by 2030, the strengthening of health systems is critical. The purpose of this study is therefore to offer supportable solutions to improve and maintain a sustainable rehabilitation service in the Nelson Mandela Metro through successful retention of staff. The aim of this study then is to investigate the influence that Human Resources (HR) processes, working conditions and leadership have had on retention of staff. A review of the available literature was conducted on possible factors that contribute to the problem and the solutions thereof. The study population included all staff that worked in rehabilitation service departments in the public health institutions in the Nelson Mandela Metro, namely; physiotherapy, occupational therapy, speech therapy and audiology. The sample constituted n=51 respondents. Data was collected via a questionnaire as the selected measurement tool. The data was analysed using the statistical data analysis software system, TIBCO Statistica (version 13), and selected methods for quantitative data. The results of the study showed that only HR processes were significantly and positively (r = 0.67, p < 0.05) related to retention. It means that satisfying the HR needs of this population is most likely to retain their employ and improve and maintain rehabilitation service delivery in the selected metro. Descriptive statistics of all the variables were also calculated and reported on. Lastly, the managerial implications of the study findings were reported on.
- Full Text:
- Date Issued: 2020
Enabling violence: the ethics of writing and reading rape in South Africa
- Authors: Lloyd, Dylan Reumen
- Date: 2020
- Subjects: Rape -- South Africa , Rape -- Fiction , Rape in literature , Rape in literature -- South Africa , Psychic trauma in literature , Post-traumatic stress disorder in literature , Dystopias in literature , Coetzee, J. M., 1940- Disgrace , South African fiction (English) -- History and criticism
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/166173 , vital:41335
- Description: This thesis is concerned with describing the stakes of reading, writing and criticising fictional depictions of rape in a country plagued by high levels of sexual violence. I consider the capacity of rape representations to cause harm to women and rape survivors, and worsen the various injuries suffered by survivors as a direct or indirect consequence of rape. The possibility of such harm prompts me to examine the role and responsibilities of readers and critics in facilitating or preventing such harm. I further discuss the potential strategies of harm prevention that readers of novelistic portrayals of rape might adopt as well as the positive outcomes that such reading strategies make possible, and which might balance out the risks that accompany them. My description of the potential harm of rape representations combines postmodern critical feminist analysis with Miranda Fricker’s work on epistemic justice and Judith Herman’s work on trauma in order to illustrate the way that these representations shape our conception of rape in a manner that affects everything from how it is enacted to our treatment of survivors to the possibility of their recovery from posttraumatic stress disorder. In order to situate my analysis in the context of South African literature and to explore the notion of responsibility in relation to the writing of scenes of rape, I utilise a close reading of J.M. Coetzee’s Disgrace. Furthermore, I discuss the utility and limits of the critical feminist strategy of using a normative critical approach to rape representations in order to prevent harm. Ultimately, I argue that the use of such a strategy, along with the development of a purpose-honed adaptive critical style, is essential to the fulfilment of our responsibilities as readers and to the prevention of further suffering.
- Full Text:
- Date Issued: 2020
- Authors: Lloyd, Dylan Reumen
- Date: 2020
- Subjects: Rape -- South Africa , Rape -- Fiction , Rape in literature , Rape in literature -- South Africa , Psychic trauma in literature , Post-traumatic stress disorder in literature , Dystopias in literature , Coetzee, J. M., 1940- Disgrace , South African fiction (English) -- History and criticism
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/166173 , vital:41335
- Description: This thesis is concerned with describing the stakes of reading, writing and criticising fictional depictions of rape in a country plagued by high levels of sexual violence. I consider the capacity of rape representations to cause harm to women and rape survivors, and worsen the various injuries suffered by survivors as a direct or indirect consequence of rape. The possibility of such harm prompts me to examine the role and responsibilities of readers and critics in facilitating or preventing such harm. I further discuss the potential strategies of harm prevention that readers of novelistic portrayals of rape might adopt as well as the positive outcomes that such reading strategies make possible, and which might balance out the risks that accompany them. My description of the potential harm of rape representations combines postmodern critical feminist analysis with Miranda Fricker’s work on epistemic justice and Judith Herman’s work on trauma in order to illustrate the way that these representations shape our conception of rape in a manner that affects everything from how it is enacted to our treatment of survivors to the possibility of their recovery from posttraumatic stress disorder. In order to situate my analysis in the context of South African literature and to explore the notion of responsibility in relation to the writing of scenes of rape, I utilise a close reading of J.M. Coetzee’s Disgrace. Furthermore, I discuss the utility and limits of the critical feminist strategy of using a normative critical approach to rape representations in order to prevent harm. Ultimately, I argue that the use of such a strategy, along with the development of a purpose-honed adaptive critical style, is essential to the fulfilment of our responsibilities as readers and to the prevention of further suffering.
- Full Text:
- Date Issued: 2020
Enactment of hands-on practical activities through using easily accessible resources in a Grade 10 Physical Science classroom
- Authors: Ndevahoma, Martha Kashike
- Date: 2020
- Subjects: Physical science -- Study and teaching (Secondary) -- Namibia
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/110229 , vital:33251
- Description: The Namibian National Subject Policy Guide for Physical Science Grade 8-12 (MoE, 2010) indicates that science teachers should be creative and innovative in producing their own teaching and learning support materials that are linked to practice. Practical activities play a major role in the learning of science. Due to the lack of science laboratory equipment, however, minimal practical activities are done in some schools especially under-resourced disadvantaged schools. It is against this background that this case study investigated the possibilities and challenges associated with the use of easily accessible resources to carry out hands-on practical activities. Underpinned by an interpretive paradigm, this study used a mixed-method approach by generating both quantitative and qualitative data. It was carried out in the Otjozondjupa region in Namibia at Wato Secondary School (pseudonym). It was conducted with a Grade 10 Physical Science class consisting of 30 learners. To generate data, questionnaires, focus group interviews, lesson observations, stimulated recall interviews, as well as learners’ journal reflections were used. Conceptions, dispositions, interest and sense-making were used as conceptual lenses while Vygotsky’s social constructivism was the theoretical framework that informed this study. Quantitative data were analysed using the conceptual lenses and were subsequently presented in tables and graphs. That is, a deductive approach to analysis was used. On the other hand, a thematic approach was used to analyse the qualitative data. That is, qualitative data were inductively analysed to come up with sub-themes and themes. The findings from this study revealed significant shifts in learners’ conceptions, dispositions and interest towards science after the use of easily accessible resources to do hands-on practical activities. Similarly, learners’ sense-making of science concepts improved. This study thus recommends that science teachers should, where possible, make use of easily accessible resources to carry out hands-on practical activities during their science lessons. During such hands-on practicals, however, emphasis should be on the development of scientific concepts.
- Full Text:
- Date Issued: 2020
- Authors: Ndevahoma, Martha Kashike
- Date: 2020
- Subjects: Physical science -- Study and teaching (Secondary) -- Namibia
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/110229 , vital:33251
- Description: The Namibian National Subject Policy Guide for Physical Science Grade 8-12 (MoE, 2010) indicates that science teachers should be creative and innovative in producing their own teaching and learning support materials that are linked to practice. Practical activities play a major role in the learning of science. Due to the lack of science laboratory equipment, however, minimal practical activities are done in some schools especially under-resourced disadvantaged schools. It is against this background that this case study investigated the possibilities and challenges associated with the use of easily accessible resources to carry out hands-on practical activities. Underpinned by an interpretive paradigm, this study used a mixed-method approach by generating both quantitative and qualitative data. It was carried out in the Otjozondjupa region in Namibia at Wato Secondary School (pseudonym). It was conducted with a Grade 10 Physical Science class consisting of 30 learners. To generate data, questionnaires, focus group interviews, lesson observations, stimulated recall interviews, as well as learners’ journal reflections were used. Conceptions, dispositions, interest and sense-making were used as conceptual lenses while Vygotsky’s social constructivism was the theoretical framework that informed this study. Quantitative data were analysed using the conceptual lenses and were subsequently presented in tables and graphs. That is, a deductive approach to analysis was used. On the other hand, a thematic approach was used to analyse the qualitative data. That is, qualitative data were inductively analysed to come up with sub-themes and themes. The findings from this study revealed significant shifts in learners’ conceptions, dispositions and interest towards science after the use of easily accessible resources to do hands-on practical activities. Similarly, learners’ sense-making of science concepts improved. This study thus recommends that science teachers should, where possible, make use of easily accessible resources to carry out hands-on practical activities during their science lessons. During such hands-on practicals, however, emphasis should be on the development of scientific concepts.
- Full Text:
- Date Issued: 2020
Encapsulation and physicochemical evaluation of efavirenz in liposomes
- Okafor, Nnamdi Ikemefuna, Nkanga, Christian I, Walker, Roderick B, Noundou, Xavier S, Krause, Rui W M
- Authors: Okafor, Nnamdi Ikemefuna , Nkanga, Christian I , Walker, Roderick B , Noundou, Xavier S , Krause, Rui W M
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/183414 , vital:43988 , xlink:href="https://doi.org/10.1007/s40005-019-00458-8"
- Description: Antiretroviral therapy remains the most efective means of managing the human immune defciency virus/acquired immune defciency syndrome (HIV/AIDS). Application of therapeutics has been hampered by factors including poor bioavailability of most anti-retroviral compounds (ARV), side efects and an alarming emergence of drug resistant strains of the virus. Recent developments and use of drug delivery systems (DDS) has shown potential for improving the pharmacological profle of ARV. Amongst these complex DDS, liposomes have been explored for delivery of ARV. In this study, we have aimed at exploring efcient encapsulation of efavirenz (EFV), a potent ARV using diferent mass ratios of crude soybean lecithin and cholesterol. The EFV-loaded liposomes (EFL) were prepared using thin flm hydration and evaluated for particle size, zeta potential (ZP), encapsulation efciency (EE%), morphology and drug release studies. Diferential scanning calorimetry (DSC), X-ray difraction (XRD), energy dispersity spectroscopy (EDS) and Fourier transform infrared (FTIR) spectroscopy were used for comprehensive physicochemical characterization of EFL. EFL exhibited high encapsulation (99%) in 1:1 crude lecithin to cholesterol mass ratio. The average particle size and Zeta Potential of EFL were found to be 411.10±7.40 nm and −53.5.3±0.06 mV, respectively. EFL showed a relatively controlled EFV release behaviour that was similar to the dissolution profle of un-encapsulated EFV. This suggests that EFL represents a promising vehicle for efective EFV delivery while providing the advantages of a nano-scaled delivery system
- Full Text:
- Date Issued: 2020
- Authors: Okafor, Nnamdi Ikemefuna , Nkanga, Christian I , Walker, Roderick B , Noundou, Xavier S , Krause, Rui W M
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/183414 , vital:43988 , xlink:href="https://doi.org/10.1007/s40005-019-00458-8"
- Description: Antiretroviral therapy remains the most efective means of managing the human immune defciency virus/acquired immune defciency syndrome (HIV/AIDS). Application of therapeutics has been hampered by factors including poor bioavailability of most anti-retroviral compounds (ARV), side efects and an alarming emergence of drug resistant strains of the virus. Recent developments and use of drug delivery systems (DDS) has shown potential for improving the pharmacological profle of ARV. Amongst these complex DDS, liposomes have been explored for delivery of ARV. In this study, we have aimed at exploring efcient encapsulation of efavirenz (EFV), a potent ARV using diferent mass ratios of crude soybean lecithin and cholesterol. The EFV-loaded liposomes (EFL) were prepared using thin flm hydration and evaluated for particle size, zeta potential (ZP), encapsulation efciency (EE%), morphology and drug release studies. Diferential scanning calorimetry (DSC), X-ray difraction (XRD), energy dispersity spectroscopy (EDS) and Fourier transform infrared (FTIR) spectroscopy were used for comprehensive physicochemical characterization of EFL. EFL exhibited high encapsulation (99%) in 1:1 crude lecithin to cholesterol mass ratio. The average particle size and Zeta Potential of EFL were found to be 411.10±7.40 nm and −53.5.3±0.06 mV, respectively. EFL showed a relatively controlled EFV release behaviour that was similar to the dissolution profle of un-encapsulated EFV. This suggests that EFL represents a promising vehicle for efective EFV delivery while providing the advantages of a nano-scaled delivery system
- Full Text:
- Date Issued: 2020
Energy management as an element of a financial efficiency strategy : a case of the City Tshwane
- Authors: Maubane, Lekgatla Donald
- Date: 2020
- Subjects: Industries -- Energy conservation -- South Africa -- City of Tshwane Metropolitan Municipality
- Language: English
- Type: Thesis , Masters , MSBE
- Identifier: http://hdl.handle.net/10948/49507 , vital:41727
- Description: The costs of operating buildings and facilities which the City of Tshwane utilises for conducting business have substantially increased due to the cost of electricity more than quadrupling in the past decade in South Africa, worsening the already constrained budgets. Research has shown that implementation of successful and sustainable energy management of built environment assets is effective in reducing costs due to resulting reduction in energy consumption and is therefore fitting to include it as an element of a financial efficiency strategy in organisations. This study aims to investigate how the City of Tshwane utilises energy management of its built environment assets to support financial efficiency and to make recommendations where necessary. Building on existing literature on energy management, it asks: How does the City of Tshwane utilise energy management of its built environment assets to support financial efficiency? In this context, energy management is defined as the continuous process of managing behavioural, organisational and technical change to improve an organisation’s energy performance. An instrument suitable for collecting qualitative data was constructed based on theories found in the reviewed literature. Interview participants were purposively sampled from departments which have direct influence on the energy management chain in the city. Relevant documents provided by the city were also reviewed to support triangulation of data. An analysis of data demonstrated that the city utilises energy management of its built environment assets to support financial efficiency. The results also indicated that there are several deficiencies in the manner in which the city currently manages energy. On this basis, it is recommended that the city installs meters for all energy consuming built environment assets; abandons the practice of accounting for energy costs as part of overheads and adopt the practice of direct costs accounting; and implements measures to review relevant strategy documents at planned intervals in order to ensure their continuing suitability.
- Full Text:
- Date Issued: 2020
- Authors: Maubane, Lekgatla Donald
- Date: 2020
- Subjects: Industries -- Energy conservation -- South Africa -- City of Tshwane Metropolitan Municipality
- Language: English
- Type: Thesis , Masters , MSBE
- Identifier: http://hdl.handle.net/10948/49507 , vital:41727
- Description: The costs of operating buildings and facilities which the City of Tshwane utilises for conducting business have substantially increased due to the cost of electricity more than quadrupling in the past decade in South Africa, worsening the already constrained budgets. Research has shown that implementation of successful and sustainable energy management of built environment assets is effective in reducing costs due to resulting reduction in energy consumption and is therefore fitting to include it as an element of a financial efficiency strategy in organisations. This study aims to investigate how the City of Tshwane utilises energy management of its built environment assets to support financial efficiency and to make recommendations where necessary. Building on existing literature on energy management, it asks: How does the City of Tshwane utilise energy management of its built environment assets to support financial efficiency? In this context, energy management is defined as the continuous process of managing behavioural, organisational and technical change to improve an organisation’s energy performance. An instrument suitable for collecting qualitative data was constructed based on theories found in the reviewed literature. Interview participants were purposively sampled from departments which have direct influence on the energy management chain in the city. Relevant documents provided by the city were also reviewed to support triangulation of data. An analysis of data demonstrated that the city utilises energy management of its built environment assets to support financial efficiency. The results also indicated that there are several deficiencies in the manner in which the city currently manages energy. On this basis, it is recommended that the city installs meters for all energy consuming built environment assets; abandons the practice of accounting for energy costs as part of overheads and adopt the practice of direct costs accounting; and implements measures to review relevant strategy documents at planned intervals in order to ensure their continuing suitability.
- Full Text:
- Date Issued: 2020
Enhanced Light-Driven Antimicrobial Activity of Cationic Poly (oxanorbornene) s by Phthalocyanine Incorporation into Polymer as Pendants
- Ahmetali, Erem, Sen, Pinar, Süer, N Ceren, Aksu, Burak, Nyokong, Tebello, Eren, Tarik, Sener, Kasim M
- Authors: Ahmetali, Erem , Sen, Pinar , Süer, N Ceren , Aksu, Burak , Nyokong, Tebello , Eren, Tarik , Sener, Kasim M
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/185976 , vital:44453 , xlink:href=" https://doi.org/10.1002/macp.202000386"
- Description: Amphiphilic poly(oxanorbornene)s are promising synthetic polymers that mimic the structural properties and antimicrobial functions of naturally occurring antimicrobial peptides. Here, poly(oxanorbornene)s bearing pendant zinc(II) phthalocyanine and triphenyl(ethyl) phosphonium functionalities are synthesized by ring-opening metathesis polymerization (ROMP). Fluorescence, singlet oxygen and triplet quantum yields of polymers are measured in dimethyl sulfoxide and aqueous medium. The singlet oxygen quantum yields of copolymers with the highest triphenyl and triethyl phosphonium content are found to be 0.29 and 0.41, respectively. Then, antimicrobial activities of polymers against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) are investigated under both dark and light conditions. Synergistic effect of zinc(II) phthalocyanine and phosphonium-containing poly(oxanorbornene) is observed that the conjugate possessing the most triphenyl phosphonium side chains has the highest activity under light against both gram-positive and gram-negative bacterial strains after 80 min irradiation, reducing the survival of E. coli or S. aureus by 99.9999%. Hemolytic concentrations of the copolymers are found between 8 and 512 µg mL−1. Scanning electron microscopy (SEM) proves that the bacteria membrane deforms after contacting with the biocidal polymer.
- Full Text:
- Date Issued: 2020
- Authors: Ahmetali, Erem , Sen, Pinar , Süer, N Ceren , Aksu, Burak , Nyokong, Tebello , Eren, Tarik , Sener, Kasim M
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/185976 , vital:44453 , xlink:href=" https://doi.org/10.1002/macp.202000386"
- Description: Amphiphilic poly(oxanorbornene)s are promising synthetic polymers that mimic the structural properties and antimicrobial functions of naturally occurring antimicrobial peptides. Here, poly(oxanorbornene)s bearing pendant zinc(II) phthalocyanine and triphenyl(ethyl) phosphonium functionalities are synthesized by ring-opening metathesis polymerization (ROMP). Fluorescence, singlet oxygen and triplet quantum yields of polymers are measured in dimethyl sulfoxide and aqueous medium. The singlet oxygen quantum yields of copolymers with the highest triphenyl and triethyl phosphonium content are found to be 0.29 and 0.41, respectively. Then, antimicrobial activities of polymers against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) are investigated under both dark and light conditions. Synergistic effect of zinc(II) phthalocyanine and phosphonium-containing poly(oxanorbornene) is observed that the conjugate possessing the most triphenyl phosphonium side chains has the highest activity under light against both gram-positive and gram-negative bacterial strains after 80 min irradiation, reducing the survival of E. coli or S. aureus by 99.9999%. Hemolytic concentrations of the copolymers are found between 8 and 512 µg mL−1. Scanning electron microscopy (SEM) proves that the bacteria membrane deforms after contacting with the biocidal polymer.
- Full Text:
- Date Issued: 2020
Enhanced photodynamic antimicrobial activity of surface modified SiNPs doped with zinc (II) phthalocyanines: The effect of antimicrobial ampicillin and extra charges from a sultone
- Magadla, Aviwe, Nyokong, Tebello
- Authors: Magadla, Aviwe , Nyokong, Tebello
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/186034 , vital:44457 , xlink:href="https://doi.org/10.1016/j.pdpdt.2020.101996"
- Description: 1-(2-Methoxyethyl) piperidine sustituted Zn phthalocyanine complex (2) is synthesised and quartenised (3). These complexes are loaded into silica nanoparticles (SiNPs) that are futher surface modified with ampicillin and 1.3-propanesultone. The photophysical and photochemical properties of the complexes and their doped conjugates were investigated in dimethylsulfoxide. The cationic complex (3) is used for photodynamic antimicrobial activity. Log reduction values of above 9 are obtained towards the photoiactivation of Staphyloccocus aureus.
- Full Text:
- Date Issued: 2020
- Authors: Magadla, Aviwe , Nyokong, Tebello
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/186034 , vital:44457 , xlink:href="https://doi.org/10.1016/j.pdpdt.2020.101996"
- Description: 1-(2-Methoxyethyl) piperidine sustituted Zn phthalocyanine complex (2) is synthesised and quartenised (3). These complexes are loaded into silica nanoparticles (SiNPs) that are futher surface modified with ampicillin and 1.3-propanesultone. The photophysical and photochemical properties of the complexes and their doped conjugates were investigated in dimethylsulfoxide. The cationic complex (3) is used for photodynamic antimicrobial activity. Log reduction values of above 9 are obtained towards the photoiactivation of Staphyloccocus aureus.
- Full Text:
- Date Issued: 2020
Enhanced photodynamic antimicrobial activity of surface modified SiNPs doped with zinc (II) phthalocyanines: the effect of antimicrobial ampicillin and extra charges from a sultone
- Magadla, Aviwe, Nyokong, Tebello
- Authors: Magadla, Aviwe , Nyokong, Tebello
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/160511 , vital:40452 , https://doi.org/10.1016/j.pdpdt.2020.101996
- Description: 1-(2-Methoxyethyl)piperidine sustituted Zn phthalocyanine complex (2) is synthesised and quartenised (3). These complexes are loaded into silica nanoparticles (SiNPs) that are futher surface modified with ampicillin and 1.3-propanesultone. The photophysical and photochemical properties of the complexes and their doped conjugates were investigated in dimethylsulfoxide. The cationic complex (3) is used for photodynamic antimicrobial activity. Log reduction values of above 9 are obtained towards the photoiactivation of Staphyloccocus aureus.
- Full Text:
- Date Issued: 2020
- Authors: Magadla, Aviwe , Nyokong, Tebello
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/160511 , vital:40452 , https://doi.org/10.1016/j.pdpdt.2020.101996
- Description: 1-(2-Methoxyethyl)piperidine sustituted Zn phthalocyanine complex (2) is synthesised and quartenised (3). These complexes are loaded into silica nanoparticles (SiNPs) that are futher surface modified with ampicillin and 1.3-propanesultone. The photophysical and photochemical properties of the complexes and their doped conjugates were investigated in dimethylsulfoxide. The cationic complex (3) is used for photodynamic antimicrobial activity. Log reduction values of above 9 are obtained towards the photoiactivation of Staphyloccocus aureus.
- Full Text:
- Date Issued: 2020
Enhancement of photodynamic antimicrobialtherapy through the use of cationic indium porphyrin conjugated to Ag/CuFe2O4 nanoparticles
- Makola, Collen L, Managa, Muthumuni, Nyokong, Tebello
- Authors: Makola, Collen L , Managa, Muthumuni , Nyokong, Tebello
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/186233 , vital:44475 , xlink:href="https://doi.org/10.1016/j.pdpdt.2020.101736"
- Description: Photodynamic antimicrobial chemotherapy (PACT) is being actively researched as a possible alternative for antimicrobial agents. This study focuses on the application of neutral indium 5-p-carboxyphenyl-10-15-20-(tri-4-pyridyl)porphyrin and cationic indium 5-p-carboxyphenyl-10-15-20-(tris-4-methylpyridyl)-porphyrin triiodide conjugated to 6-mercapo-1-hexanol functionalized Ag/CuFe2O4 magnetic nanoparticles for photo-inactivation of S. aureus bacteria. Comparative studies were done on quaternized and unquaternized indium porphyrin complexes conjugated to Ag/CuFe2O4, where log reduction of 9.27 was obtained for quaternized conjugate and 0.83 for unquaternized conjugate.
- Full Text:
- Date Issued: 2020
- Authors: Makola, Collen L , Managa, Muthumuni , Nyokong, Tebello
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/186233 , vital:44475 , xlink:href="https://doi.org/10.1016/j.pdpdt.2020.101736"
- Description: Photodynamic antimicrobial chemotherapy (PACT) is being actively researched as a possible alternative for antimicrobial agents. This study focuses on the application of neutral indium 5-p-carboxyphenyl-10-15-20-(tri-4-pyridyl)porphyrin and cationic indium 5-p-carboxyphenyl-10-15-20-(tris-4-methylpyridyl)-porphyrin triiodide conjugated to 6-mercapo-1-hexanol functionalized Ag/CuFe2O4 magnetic nanoparticles for photo-inactivation of S. aureus bacteria. Comparative studies were done on quaternized and unquaternized indium porphyrin complexes conjugated to Ag/CuFe2O4, where log reduction of 9.27 was obtained for quaternized conjugate and 0.83 for unquaternized conjugate.
- Full Text:
- Date Issued: 2020
Enriching my teaching around the inverse order relationship in unit fractions at the Grade 5 level through the inclusion of musical activities: an action research case study
- Authors: Lovemore, Tarryn Shirley
- Date: 2020
- Subjects: Mathematics -- Study and teaching (Elementary) -- South Africa -- Case studies , Interdisciplinary approach in education -- South Africa -- Case studies , Music -- Study and teaching (Elementary) -- South Africa -- Case studies , Music -- Mathematics , Musical notation -- Study and teaching (Elementary)
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/142431 , vital:38079
- Description: This research study explored how the use of musical activities, specifically based on note values, might enrich my teaching around the inverse order relation of unit fractions in my Grade 5 mathematics classroom. Much research has identified fractions as a problematic concept to teach in primary schools. Curriculum expectations of mathematics and music, as well as prior research, recognise a link between these two subject areas. Based on these connections between mathematics and music, I make the case for further research into practical ways in which music and mathematics can be integrated to support teaching and learning in the South African context. This study is located within a qualitative and interpretive framework. The concepts of integration, learning style theory, Gardner’s multiple intelligences and Bresler’s styles of arts integration guide the research. I made use of practical and theoretical activities relating to note values in music. I explored these strategies and the use of learning support materials through an action research case study, in which I engaged in cycles of trialing, reflecting, adjusting and re-trialing within the ‘case’ of my own mathematics class. Data sources comprised of my reflective journal, learner feedback, video- and audio-recorded lessons, examples of learners’ work and interviews with critical peers who observed critical moments from the video recordings. I was guided through the analysis phase by two analytical frameworks: Karsenty and Arcavi’s Six Lense Framework (SLF) and Adler and Ronda’s Mathematics Discourse in Instruction (MDI) framework which I adapted. Using three key lessons, I provide detailed descriptions of how the lessons progressed and then identify and discuss some of the key findings and recurring themes in relation to my study’s research question and goals. Through this process, I show that my integration of mathematics and musical activities helped promote active engagement amongst learners and provided them with co-equivalent opportunities to appreciate the importance of note values in music, and relate this back to understandings around the inverse order relation of unit fractions.
- Full Text:
- Date Issued: 2020
- Authors: Lovemore, Tarryn Shirley
- Date: 2020
- Subjects: Mathematics -- Study and teaching (Elementary) -- South Africa -- Case studies , Interdisciplinary approach in education -- South Africa -- Case studies , Music -- Study and teaching (Elementary) -- South Africa -- Case studies , Music -- Mathematics , Musical notation -- Study and teaching (Elementary)
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/142431 , vital:38079
- Description: This research study explored how the use of musical activities, specifically based on note values, might enrich my teaching around the inverse order relation of unit fractions in my Grade 5 mathematics classroom. Much research has identified fractions as a problematic concept to teach in primary schools. Curriculum expectations of mathematics and music, as well as prior research, recognise a link between these two subject areas. Based on these connections between mathematics and music, I make the case for further research into practical ways in which music and mathematics can be integrated to support teaching and learning in the South African context. This study is located within a qualitative and interpretive framework. The concepts of integration, learning style theory, Gardner’s multiple intelligences and Bresler’s styles of arts integration guide the research. I made use of practical and theoretical activities relating to note values in music. I explored these strategies and the use of learning support materials through an action research case study, in which I engaged in cycles of trialing, reflecting, adjusting and re-trialing within the ‘case’ of my own mathematics class. Data sources comprised of my reflective journal, learner feedback, video- and audio-recorded lessons, examples of learners’ work and interviews with critical peers who observed critical moments from the video recordings. I was guided through the analysis phase by two analytical frameworks: Karsenty and Arcavi’s Six Lense Framework (SLF) and Adler and Ronda’s Mathematics Discourse in Instruction (MDI) framework which I adapted. Using three key lessons, I provide detailed descriptions of how the lessons progressed and then identify and discuss some of the key findings and recurring themes in relation to my study’s research question and goals. Through this process, I show that my integration of mathematics and musical activities helped promote active engagement amongst learners and provided them with co-equivalent opportunities to appreciate the importance of note values in music, and relate this back to understandings around the inverse order relation of unit fractions.
- Full Text:
- Date Issued: 2020
Entrepreneurship education in the agro-biodiversity sector: a case study of G-Tech PLA roll planter value creation for a youth community of practice in Gauteng
- Authors: Thupana, Mabora
- Date: 2020
- Subjects: G-Tech PLA roll planter , Entrepreneurship -- South Africa -- Case studies , Entrepreneurship -- Study and teaching -- South Africa , Sustainable agriculture -- South Africa , Agriculture -- Environmental aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/167620 , vital:41497
- Description: Entrepreneurship is widely recognised as a basic skill that can be learned through practice. The main focus of this study was to investigate entrepreneurship education and the type of biodiversity entrepreneurial practices and opportunities that could create value for youth via the use of green technology: the PLA Roll Planter in the biodiversity and agriculture sector. The study was inspired by the need to identify and analyse knowledge, skills and entrepreneurial aspects associated with the use of green technology to strengthen entrepreneurial learning for youth participation in the green economy and green work. The study employed the use of Communities of Practice (COP) and Value Creation adopted from Wenger as theories and a focal lens to portray the contribution of technological practices to youth sustainable livelihoods and green work in the biodiversity sector. Research on the GTECH technology that was in focus in this study, the PLA Roll Planter, shows that newly introduced green innovations can increase agricultural outputs without depleting presently available resources. The study applied qualitative research approaches in a case study of the G-Tech PLA Roll Planter training and participation of a youth Community of Practice (COP) in Gauteng. It started by examining the knowledge capital as potential value embedded in the training and knowledge, skills and entrepreneurial aspects of the G-Tech PLA Roll Planter practices. The study then constructed four value creation stories of youth in the COP to understand other forms of value that were created for youth. The findings of the study depicted that the adoption of improved agricultural technology (PLA Roll Planter) has positive impacts and knowledge capital that can translate into other forms of value to support the emergence of viable economic activity in the agricultural sector. This can help increase food security and the ability to withstand risk of damaging the environment. The study shows that different forms of value were created for youth, and offers insight into how learning in communities of practice can help to advance entrepreneursip education in the biodiversity and especially the agro-ecological production sector.
- Full Text:
- Date Issued: 2020
- Authors: Thupana, Mabora
- Date: 2020
- Subjects: G-Tech PLA roll planter , Entrepreneurship -- South Africa -- Case studies , Entrepreneurship -- Study and teaching -- South Africa , Sustainable agriculture -- South Africa , Agriculture -- Environmental aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/167620 , vital:41497
- Description: Entrepreneurship is widely recognised as a basic skill that can be learned through practice. The main focus of this study was to investigate entrepreneurship education and the type of biodiversity entrepreneurial practices and opportunities that could create value for youth via the use of green technology: the PLA Roll Planter in the biodiversity and agriculture sector. The study was inspired by the need to identify and analyse knowledge, skills and entrepreneurial aspects associated with the use of green technology to strengthen entrepreneurial learning for youth participation in the green economy and green work. The study employed the use of Communities of Practice (COP) and Value Creation adopted from Wenger as theories and a focal lens to portray the contribution of technological practices to youth sustainable livelihoods and green work in the biodiversity sector. Research on the GTECH technology that was in focus in this study, the PLA Roll Planter, shows that newly introduced green innovations can increase agricultural outputs without depleting presently available resources. The study applied qualitative research approaches in a case study of the G-Tech PLA Roll Planter training and participation of a youth Community of Practice (COP) in Gauteng. It started by examining the knowledge capital as potential value embedded in the training and knowledge, skills and entrepreneurial aspects of the G-Tech PLA Roll Planter practices. The study then constructed four value creation stories of youth in the COP to understand other forms of value that were created for youth. The findings of the study depicted that the adoption of improved agricultural technology (PLA Roll Planter) has positive impacts and knowledge capital that can translate into other forms of value to support the emergence of viable economic activity in the agricultural sector. This can help increase food security and the ability to withstand risk of damaging the environment. The study shows that different forms of value were created for youth, and offers insight into how learning in communities of practice can help to advance entrepreneursip education in the biodiversity and especially the agro-ecological production sector.
- Full Text:
- Date Issued: 2020
Environment and sustainability education research as policy engagement: (re-) invigorating ‘politics as potentia’ in South Africa
- Lotz-Sisitka, Heila, Rosenberg, Eureta, Ramsarup, Presha
- Authors: Lotz-Sisitka, Heila , Rosenberg, Eureta , Ramsarup, Presha
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158230 , vital:40164 , https://0-doi.org.wam.seals.ac.za/10.1080/13504622.2020.1759511
- Description: Using a meta-review approach organized historically in relation to critical policy incidents, this paper critically reviews the process of developing and (re) invigorating Environment and Sustainability Education (ESE) (policy) research as ESE policy engagement over a 30+ year period in a rapidly transforming society, South Africa. It offers an example of long term policy-research meta-review in a context of policy flux. It adds to a body of international ESE policy studies that are seeking to understand and develop the ESE research/policy interface as this relation emerges under more complex conditions. In particular, we respond to the finding in the systematic review of ESE policy research undertaken by Aikens, McKenzie and Vaughter (2016) which reports a geographic under-representation of Africa (amongst other places) in ESE policy studies, and González-Gaudiano (2016, 118)’s insight that ESE policy research in current neo-liberally dominated political conditions and as political process, is essentially an “open, unsteady, incomplete, and relational process”.
- Full Text:
- Date Issued: 2020
- Authors: Lotz-Sisitka, Heila , Rosenberg, Eureta , Ramsarup, Presha
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158230 , vital:40164 , https://0-doi.org.wam.seals.ac.za/10.1080/13504622.2020.1759511
- Description: Using a meta-review approach organized historically in relation to critical policy incidents, this paper critically reviews the process of developing and (re) invigorating Environment and Sustainability Education (ESE) (policy) research as ESE policy engagement over a 30+ year period in a rapidly transforming society, South Africa. It offers an example of long term policy-research meta-review in a context of policy flux. It adds to a body of international ESE policy studies that are seeking to understand and develop the ESE research/policy interface as this relation emerges under more complex conditions. In particular, we respond to the finding in the systematic review of ESE policy research undertaken by Aikens, McKenzie and Vaughter (2016) which reports a geographic under-representation of Africa (amongst other places) in ESE policy studies, and González-Gaudiano (2016, 118)’s insight that ESE policy research in current neo-liberally dominated political conditions and as political process, is essentially an “open, unsteady, incomplete, and relational process”.
- Full Text:
- Date Issued: 2020
Environmental health promotion at a National Science Festival: An experiential-education based approach
- Duxbury, Theodore O, Bradshaw, Karen L, Khamanga, Sandile, Tandlich, Roman, Srinivas, Sunitha C
- Authors: Duxbury, Theodore O , Bradshaw, Karen L , Khamanga, Sandile , Tandlich, Roman , Srinivas, Sunitha C
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/440274 , vital:73762 , xlink:href="https://doi.org/10.1080/1533015X.2019.1567406"
- Description: To increase individual and communal environmental health awareness through an experiential-education project. A computer-based pre- and postintervention quiz; an educational poster; an interactive board game; and a take-home information leaflet were utilized for a school learners-centered health promotion exhibit at a National Science Festival in Grahamstown, South Africa. Out of all the participants, 55.7% were female, and 76.5% attended or had attended a government school. Participants showed significant improvement in their pre- and post educational intervention. The exhibit was effective in educating participants on environmental health, natural resources, and the impact environmental pollution has on their quality of life.
- Full Text:
- Date Issued: 2020
- Authors: Duxbury, Theodore O , Bradshaw, Karen L , Khamanga, Sandile , Tandlich, Roman , Srinivas, Sunitha C
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/440274 , vital:73762 , xlink:href="https://doi.org/10.1080/1533015X.2019.1567406"
- Description: To increase individual and communal environmental health awareness through an experiential-education project. A computer-based pre- and postintervention quiz; an educational poster; an interactive board game; and a take-home information leaflet were utilized for a school learners-centered health promotion exhibit at a National Science Festival in Grahamstown, South Africa. Out of all the participants, 55.7% were female, and 76.5% attended or had attended a government school. Participants showed significant improvement in their pre- and post educational intervention. The exhibit was effective in educating participants on environmental health, natural resources, and the impact environmental pollution has on their quality of life.
- Full Text:
- Date Issued: 2020
Epistemic Cartography: Evaluating Net-Map as a Frontline Tool for Navigating Informal Knowledge Networks
- Meterlerkamp, Luke, Schiffer, Eva
- Authors: Meterlerkamp, Luke , Schiffer, Eva
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/388009 , vital:68298 , xlink:href="https://www.ajol.info/index.php/sajee/article/view/188690"
- Description: Sustainability transitions are dependent on the development and diffusion of transformative skills and competencies. However, the prevailing notion that learning for sustainability transitions will be led by universities, technical colleges and other similar institutions is practically not feasible in much of the global south. Net-Map is a social network analysis tool that uses interviews and mapping to help people understand, visualise, discuss, and improve situations in which many different actors influence outcomes (Schiffer and Hauck, 2010). In response to the pressing need for new approaches to the development and diffusion of sustainability skills, this paper evaluates Net-Map’s suitability as a methodological tool for educators, knowledge brokers and students seeking to enhance the navigability of the often complex and uncharted occupational pathways they encounter. To do this, the research applied Net-Map to an emerging sustainability niche within the food system in order to map the learning pathways of successful sustainability pioneers. We found that Net-Map was helpful in identifying diffuse informal knowledge networks and teaching resources. Being free, quick to learn and easy to use, Net-Map is a potentially low-cost method for circumventing traditionally costly approaches to curriculum development and accreditation – assisting community-based actors to make sense of the informal knowledge and competency networks that support emerging career fields. In emerging career fields such as organic farming, where pioneer knowledge is fragmented, poorly documented and often disregarded by mainstream-science, Net-Map could be useful in the preparatory phase of curriculum planning and design, providing training designers, course conveners and facilitators with contextually informed insights.
- Full Text:
- Date Issued: 2020
- Authors: Meterlerkamp, Luke , Schiffer, Eva
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/388009 , vital:68298 , xlink:href="https://www.ajol.info/index.php/sajee/article/view/188690"
- Description: Sustainability transitions are dependent on the development and diffusion of transformative skills and competencies. However, the prevailing notion that learning for sustainability transitions will be led by universities, technical colleges and other similar institutions is practically not feasible in much of the global south. Net-Map is a social network analysis tool that uses interviews and mapping to help people understand, visualise, discuss, and improve situations in which many different actors influence outcomes (Schiffer and Hauck, 2010). In response to the pressing need for new approaches to the development and diffusion of sustainability skills, this paper evaluates Net-Map’s suitability as a methodological tool for educators, knowledge brokers and students seeking to enhance the navigability of the often complex and uncharted occupational pathways they encounter. To do this, the research applied Net-Map to an emerging sustainability niche within the food system in order to map the learning pathways of successful sustainability pioneers. We found that Net-Map was helpful in identifying diffuse informal knowledge networks and teaching resources. Being free, quick to learn and easy to use, Net-Map is a potentially low-cost method for circumventing traditionally costly approaches to curriculum development and accreditation – assisting community-based actors to make sense of the informal knowledge and competency networks that support emerging career fields. In emerging career fields such as organic farming, where pioneer knowledge is fragmented, poorly documented and often disregarded by mainstream-science, Net-Map could be useful in the preparatory phase of curriculum planning and design, providing training designers, course conveners and facilitators with contextually informed insights.
- Full Text:
- Date Issued: 2020
Essential medicine selection during the COVID-19 pandemic: Enabling access in uncharted territory
- Authors: A G Parrish
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3364 , vital:43319 , http://www.samj.org.za/index.php/samj/article/view/13086
- Description: The COVID-19 pandemic requires urgent decisions regarding treatment policy in the face of rapidly evolving evidence. In response, the South African Essential Medicines List Committee established a subcommittee to systematically review and appraise emerging evidence, within very short timelines, in order to inform the National Department of Health COVID-19 treatment guidelines. To date, the subcommittee has reviewed 14 potential treatments, and made recommendations based on local context, feasibility, resource requirements and equity. Here we describe the rapid review and evidence-to-decision process, using remdesivir and dexamethasone as examples. Our experience is that conducting rapid reviews is a practical and efficient way to address medicine policy questions under pandemic conditions.
- Full Text:
- Date Issued: 2020
- Authors: A G Parrish
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3364 , vital:43319 , http://www.samj.org.za/index.php/samj/article/view/13086
- Description: The COVID-19 pandemic requires urgent decisions regarding treatment policy in the face of rapidly evolving evidence. In response, the South African Essential Medicines List Committee established a subcommittee to systematically review and appraise emerging evidence, within very short timelines, in order to inform the National Department of Health COVID-19 treatment guidelines. To date, the subcommittee has reviewed 14 potential treatments, and made recommendations based on local context, feasibility, resource requirements and equity. Here we describe the rapid review and evidence-to-decision process, using remdesivir and dexamethasone as examples. Our experience is that conducting rapid reviews is a practical and efficient way to address medicine policy questions under pandemic conditions.
- Full Text:
- Date Issued: 2020
Essential services in light of the 2014 Labour Law amendments
- Authors: Seshoka, Lesiba Job
- Date: 2020
- Subjects: Labor laws and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: http://hdl.handle.net/10948/47427 , vital:39990
- Description: The topic of the treatise is “Essential Services in light of the 2014 Labour Law amendments”. It aims to investigate the state of essential services in the wake of the 2014 changes to the Labour Relations Act. As Pillay1 puts it, Furthermore, 15 years under our new essential services regime, it is time for reflection to preserve and improve what works and to fix what does not. The treatment of essential services is not playing as planned. In the meantime, notwithstanding the prohibition of strikes in essential services, they occur with disconcerting frequency, duration and intensity”. From these, one can deduce that prior to the changes effected in 2014, there have been serious challenges facing essential service personnel and employers which necessitated a change in legislation. The question, which this treatise tries to answer, is whether such change in legislation would in all likelihood addresses the challenges, which faced essential service employers and employees before it was enacted.
- Full Text:
- Date Issued: 2020
- Authors: Seshoka, Lesiba Job
- Date: 2020
- Subjects: Labor laws and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: http://hdl.handle.net/10948/47427 , vital:39990
- Description: The topic of the treatise is “Essential Services in light of the 2014 Labour Law amendments”. It aims to investigate the state of essential services in the wake of the 2014 changes to the Labour Relations Act. As Pillay1 puts it, Furthermore, 15 years under our new essential services regime, it is time for reflection to preserve and improve what works and to fix what does not. The treatment of essential services is not playing as planned. In the meantime, notwithstanding the prohibition of strikes in essential services, they occur with disconcerting frequency, duration and intensity”. From these, one can deduce that prior to the changes effected in 2014, there have been serious challenges facing essential service personnel and employers which necessitated a change in legislation. The question, which this treatise tries to answer, is whether such change in legislation would in all likelihood addresses the challenges, which faced essential service employers and employees before it was enacted.
- Full Text:
- Date Issued: 2020
Establishment of an information technology risk management framework within food manufacturing enterprises in South Africa
- Authors: Sandi, Siyabulela
- Date: 2020
- Subjects: Business enterprises -- Computer networks -- Security measures , Information technology -- Security measures Computer security Risk management
- Language: English
- Type: Thesis , Masters , MPhil
- Identifier: http://hdl.handle.net/10948/47128 , vital:39812
- Description: Enterprises of all kinds, regardless of the sector, are directly or indirectly dependent on Information Technology (IT) to carry out their daily activities. With this in mind, and correlated with the problem statement that it is “the lack of IT governance principles that lead to exposing enterprises to IT-related threats, vulnerabilities, and risks”, the objective of this study was to establish an Information Technology Risk Management Framework for enterprises within the Food manufacturing industry in South Africa that will ensure that IT-related threats, vulnerabilities, and risks are properly managed. In order to accomplish this, the research followed a process called design science research. The design science research paradigm was used to create a design artificial artefact in the form of a framework. The Nelson Mandela University – Design Science Framework Methodology (NMU-DSFM) was adopted since the objective of the study was to develop a framework. The study has revealed that enterprises within the sector are indeed lagging behind in terms of IT governance principles, hence an artefact called the IT Risk Management Framework for Enterprises within Food Manufacturing Industries in South Africa was developed.
- Full Text:
- Date Issued: 2020
- Authors: Sandi, Siyabulela
- Date: 2020
- Subjects: Business enterprises -- Computer networks -- Security measures , Information technology -- Security measures Computer security Risk management
- Language: English
- Type: Thesis , Masters , MPhil
- Identifier: http://hdl.handle.net/10948/47128 , vital:39812
- Description: Enterprises of all kinds, regardless of the sector, are directly or indirectly dependent on Information Technology (IT) to carry out their daily activities. With this in mind, and correlated with the problem statement that it is “the lack of IT governance principles that lead to exposing enterprises to IT-related threats, vulnerabilities, and risks”, the objective of this study was to establish an Information Technology Risk Management Framework for enterprises within the Food manufacturing industry in South Africa that will ensure that IT-related threats, vulnerabilities, and risks are properly managed. In order to accomplish this, the research followed a process called design science research. The design science research paradigm was used to create a design artificial artefact in the form of a framework. The Nelson Mandela University – Design Science Framework Methodology (NMU-DSFM) was adopted since the objective of the study was to develop a framework. The study has revealed that enterprises within the sector are indeed lagging behind in terms of IT governance principles, hence an artefact called the IT Risk Management Framework for Enterprises within Food Manufacturing Industries in South Africa was developed.
- Full Text:
- Date Issued: 2020
Ethics amongst public sector built environment professionals: Gauteng Provincial Government
- Authors: Magabuko, Thando Zuko
- Date: 2020
- Subjects: Civil service ethics -- South Africa -- Gauteng Public administration -- Moral and ethical aspects -- South Africa -- Gauteng
- Language: English
- Type: Thesis , Masters , MSBE
- Identifier: http://hdl.handle.net/10948/49484 , vital:41725
- Description: This study sought to establish whether there are merits to the perceptions of lack of professional, personal and corporate ethics amongst Built Environment professionals in Gauteng Provincial Government (GPG), and to decipher the variance in the lack of ethics per built environment discipline. It also sought to propose ways to address the lack thereof, if it exists. The method that was utilised in this study is the quantitative research technique. Descriptive statistical techniques were used to analyse the data. The gathering of the primary data was done through the use of semi-structured questionnaires. The questionnaire was divided into three sections, with A being the demographical information, B the closed questions on the type of ethics (namely professional, personal and corporate) and C the open-ended questions on the type of ethics (namely professional, personal and corporate). This study has revealed that the lack of ethics amongst the professionals is marginal; but enforcement of the code of conduct and the willingness of senior management to address the ethical issues indeed varies per GPG department. The implications of the findings are that although unethical conduct is not rampant amongst built-environmental professionals in the Gauteng Provincial Government, it needs to be addressed and contained through mechanisms, such as training, the conducting of awareness campaigns and enforcement by senior management. If management can enforce the department’s code of conduct; and if professional bodies conduct awareness campaigns, the issue of unethical conduct could be reduced.
- Full Text:
- Date Issued: 2020
- Authors: Magabuko, Thando Zuko
- Date: 2020
- Subjects: Civil service ethics -- South Africa -- Gauteng Public administration -- Moral and ethical aspects -- South Africa -- Gauteng
- Language: English
- Type: Thesis , Masters , MSBE
- Identifier: http://hdl.handle.net/10948/49484 , vital:41725
- Description: This study sought to establish whether there are merits to the perceptions of lack of professional, personal and corporate ethics amongst Built Environment professionals in Gauteng Provincial Government (GPG), and to decipher the variance in the lack of ethics per built environment discipline. It also sought to propose ways to address the lack thereof, if it exists. The method that was utilised in this study is the quantitative research technique. Descriptive statistical techniques were used to analyse the data. The gathering of the primary data was done through the use of semi-structured questionnaires. The questionnaire was divided into three sections, with A being the demographical information, B the closed questions on the type of ethics (namely professional, personal and corporate) and C the open-ended questions on the type of ethics (namely professional, personal and corporate). This study has revealed that the lack of ethics amongst the professionals is marginal; but enforcement of the code of conduct and the willingness of senior management to address the ethical issues indeed varies per GPG department. The implications of the findings are that although unethical conduct is not rampant amongst built-environmental professionals in the Gauteng Provincial Government, it needs to be addressed and contained through mechanisms, such as training, the conducting of awareness campaigns and enforcement by senior management. If management can enforce the department’s code of conduct; and if professional bodies conduct awareness campaigns, the issue of unethical conduct could be reduced.
- Full Text:
- Date Issued: 2020