Computational search for nature-derived dual-action inhibitors of HIV-1 reverse transcriptase and integrase: a potential strategy to mitigate drug resistance progression
- Authors: Mwiinga, Luyando
- Date: 2024-10-11
- Subjects: HIV (Viruses) , Reverse transcriptase , Antiretroviral agents , RDKit , Drug resistance , Docking
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/463930 , vital:76458
- Description: Human immunodeficiency virus Type 1 (HIV-1) is a devastating viral infection affecting millions worldwide and presents significant challenges in treatment and management. In 2022, approximately 39 million people were living with HIV with Sub-Saharan Africa having two thirds of these infections. Devastatingly, there were approximately 300 000 HIV/AIDS related deaths in Sub-Saharan Africa alone in 2022 alone. Antiretroviral therapy (ART) which is fundamental for HIV treatment, comprises of a combination of drugs such as nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTs), protease inhibitors (PIs) and integrase strand transfer inhibitors (INSTIs). However, although 28.7 million people out of the estimated 38.4 million people living with HIV in 2021 were receiving ART, the emergence of drug-resistant strains further complicates treatment efforts, highlighting the need for novel therapeutic approaches. This study aimed to address the challenges raised by drug resistance and significant side effects by identifying potential dual inhibitors against HIV-1 Reverse Transcriptase (RT) and Integrase (IN) using in silico techniques. RT RNase H and IN were chosen as targets for their shared dependency on Mg2+ ions within their active sites, which are crucial for catalytic activity. The selection of dual inhibitors was motivated by the fact that the virus would need to replicate at two points simultaneously to develop resistance, making it less likely. The objectives of this study included the creation of a natural derivative compound library using RDKit with the aid of SciFinder, utilizing (-)-epigallocatechin-3-O-gallate (EGCG), because of its dual inhibitory effects against RT and IN, as indicated by a study conducted by Sanna et al. 2019. The natural derivatives were chosen to take advantage of their chemical diversity and to explore potential novel therapeutic options for combating HIV drug resistance. The compound library created comprised of 125 203 compounds. Then docking studies were conducted to assess proteinligand binding. After the correlation of the RT and IN docking studies, 288 compounds were filtered to have potential dual inhibitory activity. Then quantitative estimation of druggability (QED) analysis identified three compounds with superior properties compared to EGCG and FDAapproved drug raltegravir (RAL). Molecular docking simulations revealed interactions between the inhibitors and the key active site residues of RT and IN, along with the chelation of at least one 3 Mg2+, suggesting the potential for enzymatic disruption. Furthermore, molecular dynamic (MD) simulations were then conducted to assess protein-ligand system behavior, through RMSD and RMSF analysis. The RMSD analysis uncovered instability in the IN-Sci30703 complex, leading to its exclusion as a potential dual action inhibitor. RMSF analysis for IN showed that all the inhibitors had the ability to limit the flexibility of the catalytic loop which is essential for catalytic activity. Therefore, further in vitro studies are required to evaluate the effectiveness of the remaining two EGCG derivatives (Sci33211 and Sci48919) in inhibiting RT and IN through the chelation of at least one Mg2+ ion to determine if they have superior dual inhibitory effects compared to EGCG. This study adds to the ongoing efforts to develop effective strategies against HIV-1 drug resistance and emphasizes the importance of continued research in this field. , Thesis (MSc) -- Faculty of Science, Biochemistry, Microbiology & Bioinformatics, 2024
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- Date Issued: 2024-10-11
Connecting culture and science: Utilising Oshikundu and Ombidi in biology lessons on diffusion and osmosis
- Authors: Shilomboleni, Helena Ilena Ndilimeke
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/463636 , vital:76427
- Description: Various science curricula including that of Namibia recommend that teaching and learning should start with the knowledge and experiences of learners from their homes and communities. These experiences are the learners’ Indigenous Knowledge (IK) which should be integrated to make science accessible and relevant to learners from diverse sociocultural backgrounds. This suggests that science teachers are encouraged to integrate IK into their teaching to help learners grasp abstract concepts and construct their own understanding of phenomena. However, much literature has revealed that many teachers find it difficult to integrate IK into their science teaching. Some scholars further argue that teachers lack the pedagogical content knowledge to effectively integrate IK. I assume this could be because the science curriculum is not explicit on how IK should be integrated into science teaching. It is against this backdrop that in this study I mobilised the Indigenous technologies of making oshikundu and preserving ombidi to support Grade 10 Biology learners in talking and making sense of diffusion and osmosis. This study was underpinned by the interpretivist and Indigenous research paradigms. Within the Indigenous research paradigm, I focused on Ubuntu as a perspective. A qualitative case study was employed and conducted at a certain school in the northern part of Namibia, in the Ohangwena region. I worked with a group of 35 Grade 10 learners, two Indigenous Knowledge Custodians (KCs) who are the custodians of cultural heritage and one Biology teacher (critical friend) as participants in this study. Qualitative data were collected using a focus group interview (sharing circle), group activity, participatory observation, and learners’ journal reflections. Vygotsky’s sociocultural theory together with Ogunniyi’s Contiguity Argumentative Theory were used as lenses to analyse data. The findings of the study revealed that during the IKCs’ practical demonstrations learners were able to identify science concepts embedded in the cultural practices of making oshikundu and preserving ombidi. The findings further revealed that the practical demonstrations enhanced active participation and interactions among learners. I thus recommend that teachers should tap into IKCs’ cultural heritage by inviting them into their classrooms to make science relevant, accessible and meaningful to learners. , Thesis (MEd) -- Faculty of Education, Secondary and Post-School Education, 2024
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- Date Issued: 2024-10-11
Decolonizing the curriculum: a case study of the Humanities Faculty at Rhodes University
- Authors: Machiha, Nigel
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/466005 , vital:76676
- Description: This thesis explores students’ experiences in the Faculty of Humanities at Rhodes University, specifically focusing on their perspectives on decolonization. The thesis has two central aims: first, to investigate the students’ general understanding of decolonizing the curriculum and second, to examine their experiences within the Humanities Faculty and their perception of how decolonized the faculty is. Through in-depth interviews with students in the faculty, the study aimed to answer questions regarding students’ thoughts on the decolonization of university curricula and their experiences within the Faculty of Humanities at Rhodes University. The findings reveal diverse student views on decolonization, with definitions of decolonization provided by students touching on topics such as the Africanization or Indigenization of the curriculum, systemic transformation, the importance of unlearning colonial ideologies and the relationship between language and decolonization. The students’ perceptions of the level of decolonization they witness in the faculty are categorized along three lines: the views of those who believe the departments they interact with are decolonized, the views of those who think efforts are being made but more progress is needed, and those who see no evidence of decolonization. The thesis highlights that while some students believe that positive steps towards decolonization are being taken, others express scepticism and call for a more diverse representation of scholars and scholarship and a departure from traditional Eurocentric approaches. , Thesis (MA) -- Faculty of Humanities, Political and International Studies, 2024
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- Date Issued: 2024-10-11
Design and fabrication of millifluidic devices for maintenance of tissue cultures under perfusion
- Authors: Buthelezi, Khanyisile Thando Fatima
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464358 , vital:76504
- Description: Restricted access. Expected release in 2026. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2024
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- Date Issued: 2024-10-11
Determining the relationship between perceptions of HIV susceptibility and HIV status of women who have sex with women
- Authors: Zuccarini, Aimee Frances
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/465859 , vital:76660
- Description: Background: Adolescent girls and young women (AGYW) who have sex with women (WSW) are at potential risk for HIV, contradicting the common belief that WSW have low HIV susceptibility, a misperception persists despite evidence to refute it. Objective: This study examined the relationship between perceived HIV susceptibility and actual HIV status among South African AGYW WSW, focusing on those reporting at least one same-sex sexual experience, using data from the DREAMS Evaluation survey. Methods/Design: An archival study employing the Expanded Health Beliefs Model (EHBM) analysed secondary de-identified data from the DREAMS Evaluation Survey with a multistage stratified cluster sampling design. The sample comprised 18,296 AGYW aged 12-24 across four districts in South Africa, including 1,362 identified as WSW. ANOVA was conducted using SPSS 28 to explore the relationship between perceived HIV susceptibility and actual HIV status. Results: WSW’s perceived HIV susceptibility was low (38.5%) to very low (25%), while HIV prevalence in this sample/group was 15.9%. No significant relationship was found between perceived HIV susceptibility and actual HIV status (p = 0.126). Conclusions: WSW do not perceive themselves as being susceptible to contracting HIV despite the high prevalence of the virus in this group. Public health interventions should focus on raising awareness about risk factors, such as low use of barrier protection, substance use, and other behaviours highlighted in the literature, to better inform and protect this population. , Thesis (MA) -- Faculty of Humanities, Psychology, 2024
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- Date Issued: 2024-10-11
Developing transformative community-university partnerships: a participatory assessment of Rhodes University community engagement monitoring and evaluation system
- Authors: Nkwinti, Nosipho
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/463592 , vital:76423
- Description: This thesis comprehensively explores transformative community engagement (CE) in higher education, focusing on the context of collaborative community-university partnerships (CUPs) in Makhanda, South Africa. The study aimed to assess and revise the monitoring and evaluation (M&E) system of the Rhodes University Community Engagement division to foster a transformation in CUPs. The research employs an exploratory design to understand the complexities of CUPs and improve the M&E systems by generating new concepts and ideas. A variety of qualitative methods, including focus groups, interviews, and document analysis, were utilized to engage community partners, student leaders, and Rhodes University Community Engagement staff. The participatory approach to research was critical, involving stakeholders in finalizing the M&E framework to ensure it met the shared objective. Data analysis was conducted inductively, with themes emerging from the data to facilitate a bottom-up understanding of the research questions. The study found that diverse community engagement projects at Rhodes University enhanced partnerships between the university and the local community in Makhanda. Participants shared varied understandings and experiences of Community Engagement. They saw CE as crucial for bridging the university-community divide and aiding local development. Furthermore, the research identified the need for a Monitoring and Evaluation system that supports collaborative Community-University Partnerships. Participants collaboratively developed and refined this M&E system, ensuring it aligns with community needs and promotes sustained development in Makhanda. Challenges such as diverse perspectives, logistical issues in focus groups, and power dynamics were also highlighted and addressed through an inclusive and participatory approach. This research highlights the elaborate distinctions of community engagement and the importance of such partnerships in fostering sustainable societal development. The framework developed has practical implications for shaping policies, fostering inclusivity, and promoting responsive university-community engagement that is aligned with community needs. The research concludes by suggesting the value of inclusivity, communication, and a flexible adaptation of practices to maximize the benefits of such partnerships. Future research could explore the longitudinal impacts of university-community partnerships, examine the scalability of the current framework to other universities, and evaluate the long-term socio-economic outcomes of the engaged citizen program on the community. , Thesis (MEd) -- Faculty of Education, Secondary and Post-School Education, 2024
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- Date Issued: 2024-10-11
Developing transformative community-university partnerships: a participatory assessment of Rhodes University community engagement monitoring and evaluation system
- Authors: Nkwinti, Nosipho
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/463603 , vital:76424
- Description: This thesis comprehensively explores transformative community engagement (CE) in higher education, focusing on the context of collaborative community-university partnerships (CUPs) in Makhanda, South Africa. The study aimed to assess and revise the monitoring and evaluation (M&E) system of the Rhodes University Community Engagement division to foster a transformation in CUPs. The research employs an exploratory design to understand the complexities of CUPs and improve the M&E systems by generating new concepts and ideas. A variety of qualitative methods, including focus groups, interviews, and document analysis, were utilized to engage community partners, student leaders, and Rhodes University Community Engagement staff. The participatory approach to research was critical, involving stakeholders in finalizing the M&E framework to ensure it met the shared objective. Data analysis was conducted inductively, with themes emerging from the data to facilitate a bottom-up understanding of the research questions. The study found that diverse community engagement projects at Rhodes University enhanced partnerships between the university and the local community in Makhanda. Participants shared varied understandings and experiences of Community Engagement. They saw CE as crucial for bridging the university-community divide and aiding local development. Furthermore, the research identified the need for a Monitoring and Evaluation system that supports collaborative Community-University Partnerships. Participants collaboratively developed and refined this M&E system, ensuring it aligns with community needs and promotes sustained development in Makhanda. Challenges such as diverse perspectives, logistical issues in focus groups, and power dynamics were also highlighted and addressed through an inclusive and participatory approach. This research highlights the elaborate distinctions of community engagement and the importance of such partnerships in fostering sustainable societal development. The framework developed has practical implications for shaping policies, fostering inclusivity, and promoting responsive university-community engagement that is aligned with community needs. The research concludes by suggesting the value of inclusivity, communication, and a flexible adaptation of practices to maximize the benefits of such partnerships. Future research could explore the longitudinal impacts of university-community partnerships, examine the scalability of the current framework to other universities, and evaluate the long-term socio-economic outcomes of the engaged citizen program on the community. , Thesis (MEd) -- Faculty of Education, Secondary and Post-School Education, 2024
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- Date Issued: 2024-10-11
Development of a novel agar-based microtitre plate system for construction of consistent biofilm models and subsequent use in antimicrobial screening
- Authors: Davies, Michaela Roxanne
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464369 , vital:76505
- Description: Restricted access. Expected release in 2026. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2024
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- Date Issued: 2024-10-11
Development of efficient protocols to generate breast cancer spheroids for application in drug discovery
- Authors: Ntsoyi, Nomaphelo Ntombikayise
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464380 , vital:76506
- Description: Restricted access. Expected release in 2026. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2024
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- Date Issued: 2024-10-11
Do the Cytochrome P450 1A2 allele variations have an effect on the structure and function of the protein?
- Authors: Mondile, Queenie
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/463907 , vital:76456
- Description: Access restricted. Expected release in 2025. , Thesis (MSc) -- Faculty of Science, Biochemistry, Microbiology & Bioinformatics, 2024
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- Date Issued: 2024-10-11
Drug development and animal welfare: bioethical analysis of emergency situations and the research settings
- Authors: Msimang, Asante
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/461852 , vital:76245
- Description: Access restricited. Expected release date 2026. , Thesis (Msc (Pharmacy)) -- Faculty of Pharmacy, Pharmacy, 2024
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- Date Issued: 2024-10-11
Eastern Cape jazz heritage: the jazz tradition and veteran musicians of East London and Zwelitsha
- Authors: Giyose, Thandikile Qhawekazi
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/465964 , vital:76672
- Description: This thesis explores the memories of musicians and music collectors as evident in the performance of traditional jazz songs in East London and Zwelitsha. I ask, how may research on musicians and their songs develop new knowledge about the aesthetics of jazz in the Eastern Cape and contribute to transforming notions of memories and archives? I argue that these songs are repositories of collective memory about the musical pasts of Eastern Cape modernity. We commemorate cities such as Johannesburg and Cape Town for nurturing South African jazz but what is known of the jazz musicians who remained in the country, and those musicians whose musicality was nurtured in the Eastern Cape? Where are the stories of their musical journeys and compositions located? How did these musicians contribute to the development of a form of South African jazz, which now represents and forms a large part of our heritage and our various identities as musicians, fans and performers in South Africa? In-depth and semi-structured interviews were conducted with veteran jazz musicians, jazz music collectors and members of the younger generation of jazz musicians who are originally from or reside in East London and Zwelitsha, Eastern Cape. The research follows a qualitative methodology, using an exploratory case study with a focused ethnographic approach, to understand how these musicians’ songs have retained memories of their lived experiences. The research is derived from oral histories of musicians to understand how the songs survive in the collective memory of musicians and their fans, contributing to the preservation of Eastern Cape’s jazz heritage. , Thesis (MA) -- Faculty of Humanities, Music and Musicology, 2024
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- Date Issued: 2024-10-11
Elucidating the effect of potential mitochondrial toxicants on glucose handling and insulin signalling in skeletal muscle and liver cell lines in vitro
- Authors: Kuretu, Auxiliare
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/461830 , vital:76243
- Description: Access restricited. Expected release date 2026. , Thesis (Msc (Pharmacy)) -- Faculty of Pharmacy, Pharmacy, 2024
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- Date Issued: 2024-10-11
Encroaching species are stronger anisohydric “water spenders” under elevated CO2 conditions: implications for savanna seedling establishment rates
- Authors: Reynolds, Liam Macleod
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/465091 , vital:76572
- Description: Plant water transport systems play a fundamental role in the productivity and survival of terrestrial plants due to the vascular architecture placing a physical limit on metabolic function. Savannas have high variability in rainfall, leading multiple studies to suggest that plant water-use strategies are key mechanisms affecting seedling establishment rates. Many savannas are seeing a directional shift towards an increase in the abundance of certain woody species through a process known as bush encroachment, which has been largely attributed to the fertilising effect of rising atmospheric [CO2] on C3 trees. These species are classified as encroachers. While there have been multiple studies investigating changes in the physiology of savanna species under elevated CO2 (eCO2), few have examined how climate and eCO2 affects the fundamental water-use strategies in the seedling stage, a crucial demographic bottleneck. Here, this research provides valuable insights into the mechanisms behind bush encroachment in the context of eCO2 using results from a pot experiment at the Rhodes University Elevated CO2 facility and a field experiment. All species showed water use strategies characteristic of anisohydric “water-spenders”, however, the vulnerability to embolism and rates of water-use were different between encroachers and non-encroachers. Encroachers are better at taking advantage of water pulses, particularly under eCO2 and grass competition. This comes at the cost of higher xylem vulnerability during drought, leading to reductions in conductance when exposed to heavy water stress. The response of the photosynthetic parameters mirrored this, with encroaching species had higher rates of photosynthesis and photosystem II quantum yield than non-encroachers under the well-watered treatments. Field experiments revealed that small trees are particularly vulnerable to drought stress, when compared to medium and large trees. The outcomes of this complex response will largely depend on the extent of changes to biotic and abiotic factors across spatial and temporal zones caused by climate change. This research highlights potential hydraulic mechanisms contributing to the increase in bush encroachment, as well as providing important insights into the determinant factors that make a savanna species capable of encroachment. , Thesis (MSc) -- Faculty of Science, Botany, 2024
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- Date Issued: 2024-10-11
Enhancing licence plate recognition for a robust vehicle re-identification system
- Authors: Boby, Alden Zachary
- Date: 2024-10-11
- Subjects: Automobile theft South Africa , Deep learning (Machine learning) , Object detection , YOLOv7 , YOLO , Pattern recognition systems , Image processing Digital techniques , Automobile license plates
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464322 , vital:76501
- Description: Vehicle security is a growing concern for citizens of South Africa. Law enforcement relies on reports and security camera footage for vehicle identification but struggles to match the increasing number of carjacking incidents and low vehicle recovery rates. Security camera footage offers an accessible means to identify stolen vehicles, yet it often poses hurdles like anamorphic plates and low resolution. Furthermore, depending on human operators proves inefficient, requiring faster processes to improve vehicle recovery rates and trust in law enforcement. The integration of deep learning has revolutionised object detection algorithms, increasing the popularity of vehicle tracking for security purposes. This thesis investigates advanced deep-learning methods for a comprehensive vehicle search and re-identification system. It enhances YOLOv7’s algorithmic capabilities and employs preprocessing techniques like super-resolution and perspective correction via the Improved Warped Planar Object Detection network for more effective licence plate optical character recognition. Key contributions include a specifically annotated dataset for training object detection models, an optical character recognition model based on YOLOv7, and a method for identifying vehicles in unrestricted data. The system detected rectangular and square licence plates without prior shape knowledge, achieving a 98.7% character recognition rate compared to 95.31% in related work. Moreover, it outperformed traditional optical character recognition by 28.25% and deep-learning EasyOCR by 14.18%. Its potential applications in law enforcement, traffic management, and parking systems can improve surveillance and security through automation. , Thesis (MSc) -- Faculty of Science, Computer Science, 2024
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- Date Issued: 2024-10-11
Evaluating baculovirus mixtures against false codling moth Thaumatotibia leucotreta Meyrick. (Lepidoptera: Tortricidae)
- Authors: Tole, Siviwe
- Date: 2024-10-11
- Subjects: False codling moth Biological control , Baculoviruses , Integrated pest management , Natural pesticides , Granulovirus
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/463996 , vital:76464
- Description: False codling moth (FCM), Thaumatotibia leucotreta, is an important pest of citrus, stone fruit, avocados, peppers, and other important agricultural crops in southern Africa. Baculovirus-based biopesticides are components in an integrated pest management (IPM) programme to manage the pest in the field. Cryptogran™ and Cryptex™ which are CrleGV-SA based-biopesticides have been effective in the control of T. leucotreta for the past 15 years. Recently, CrpeNPV-based Multimax™ and Codlmax™ have been commercialised to control T. leucotreta and other important agricultural pests. Despite these viruses being relatively host-specific and safe to humans and animals in comparison to chemical insecticides, their application is hindered by their slow speed of kill, sensitivity to UV light, and the potential for insect resistance. Research investigating the effects of mixed baculoviral interactions against target pests has been a growing field of interest due to their potential to overcome such shortcomings. Previous studies using a combination of CrleGV-SA and CrpeNPV against T. leucotreta observed a reduction in lethal concentration in laboratory bioassays, indicating that such mixtures may have the potential for application in the field. This has led to the motivation to investigate further interactions between CrleGV-SA in combination with CrpeNPV, CpGV-M, and HearNPV-Au to understand better how these viruses interact and to determine whether synergistic, additive, or antagonistic interactions can occur against T. leucotreta. The outcome of these interactions will inform researchers and farmers about best practices concerning these viruses should they be combined against T. leucotreta in the future. Prior to performing mixed baculovirus infections in laboratory bioassays, oligonucleotides targeting unique regions in the viral genomes of CrleGV-SA, CrpeNPV, CpGV-M, and HearNPV-Au were designed using Primer-BLAST. The specificity of these oligonucleotides was further tested in silico using Geneious R11 software (11.1.5). The stocks of CrpeNPV, CpGV-M, and HearNPV-Au were purified using crude OB extraction from diseased C. peltastica, C. pomonella, and H. armigera larval cadavers provided by River Bioscience (Pty) Ltd (Gqeberha, South Africa). The stock of CrleGV-SA was purified using crude OB extraction from infected T. leucotreta cadavers. Subsequently, the unique oligonucleotides were used in PCR assays to detect if the samples contained the baculoviruses of interest. Amplicons of the expected sizes were generated indicating the presence of CrleGV-SA, CrpeNPV, CpGV-M, and HearNPV-Au in each of the samples. The OBs were counted using darkfield microscopy and a counting chamber before the single and mixed infections were initiated against T. leucotreta neonate larvae. Surface-dose biological assays were used to evaluate the relative virulence in terms of lethal concentration of CrleGV-SA, CrpeNPV, and CpGV-M, alone against T. leucotreta. After 7 days, the dose mortality data was analysed using “drc” in R studio and the LC50 and LC90 were compared amongst each virus. The CrleGV-SA treatment was estimated to be the most virulent in comparison to CrpeNPV and CpGV-M. A dose discriminate assay confirmed that HearNPV does not cause mortality in T. leucotreta. Similarly, the relative virulence in terms of lethal concentration of CrleGV-SA in various ratios in combination with CrpeNPV, CpGV-M, and HearNPV-Au was determined using 7-day surface dose biological assays. The CrleGV/CrpeNPV was the most virulent mixture with lower LC50 and LC90 values measured in comparison to CrleGV/CpGV and CrleGV/HearNPV, respectively. The Tammes Bakuniak graphic method confirmed the CrleGV/CrpeNPV, CrleGV/CpGV, and CrleGV/HearNPV mixtures to be antagonistic against T. leucotreta neonate larvae in terms of lethal concentration. The last aspect of the study was to determine the probable cause of larval death. A modified CTAB protocol was used to extract genomic DNA from neonate-sized T. leucotreta cadavers collected in single and mixed infection assays. The gDNA served as templates in PCR assays using the unique oligonucleotides. In single infections, the presence of CrleGV-SA in CrpeNPV and HearNPV inoculated larvae was observed. The results suggest possible covert infections of CrleGV-SA in the T. leucotreta colony which may be caused by virus infection or an unknown stress factor. The results from the mixed infections showed the presence of each virus in all replicates except for the CrleGV/CpGV and CrleGV/HearNPV mixtures. In the CrleGV/CpGV mixture, only CrleGV-SA was present in the last replicate, suggesting a possible competition for host resources. In the CrleGV/HearNPV mixture, only CrleGV-SA was detected in all 3 replicates, suggesting that HearNPV did not have any effect and the larvae died of the CrleGV-SA infection. This is the first study to report mixtures of CrleGV-SA in combination with CpGV-M and HearNPV-Au against T. leucotreta neonate larvae. Despite the antagonistic interactions observed in the evaluated mixtures, this study has laid a foundation to further investigate how these viruses interact in dual infections for the improved control of T. leucotreta. This may be done by evaluating different ratios and combinations of baculoviruses to those used in this study. , Thesis (MSc) -- Faculty of Science, Biochemistry, Microbiology & Bioinformatics, 2024
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- Date Issued: 2024-10-11
Evaluation of education quality in the Edulink education for sustainable development training programme in Namibia
- Authors: Johannes, Maria Nandehanda
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/463702 , vital:76433
- Description: Restricted access. Expected release 2026. , Thesis (MEd) -- Faculty of Education, Primary and Early Childhood Education, 2024
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- Date Issued: 2024-10-11
Evaluation of screening methods for aptamer-target affinity: towards biosensors for the detection of histamine and human chorionic gonadotropin
- Authors: Yu, Jialin
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464395 , vital:76507
- Description: Restricted access. Expected release in 2026. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2024
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- Date Issued: 2024-10-11
Evaluation of traditional and residual momentum strategies during the Covid period on the Johannesburg Stock Exchange
- Authors: Yengwa, Mphathi Lubabalo
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/462834 , vital:76339
- Description: Traditional momentum is a concept which was first discovered by Jegadeesh and Titman (1993), defined as a tendency of stocks to experience a continuation in their relative performance. A stock that performed relatively well will continue to perform relatively well, and vice versa. It has been observed by other researchers that during market crises, traditional momentum tends to produce large negative returns for investors, defined as a momentum crash. To mitigate momentum crashes, many researchers have developed new momentum strategies which have better performance than traditional momentum during market crises; such strategies include residual momentum. While both residual and traditional momentum have been studied in international markets and locally, the performance of both the residual and traditional momentum strategies have not been examined in the most recent Covid-fuelled financial crisis on the Johannesburg Stock Exchange. The study compares the performance of hypothetical long-only winner traditional and residual momentum portfolios (from 2018–2022) using various risk metrics, which include the tracking error, Sharpe ratio, Jensen’s alpha and information ratio. To compare the statistical significance of the difference in mean returns of residual and traditional momentum strategies to the benchmark (FTSE/Johannesburg Stock Exchange (JSE) Top 40) the study uses Welch’s t-test. The study uses an Auto regressive distributed lag (ARDL) regression to examine the effect that various market conditions (bull market, bear market and extreme volatility) have on the returns of residual and traditional momentum strategies. Given the limited period examined in this study, the Monte Carlo simulation was used to extrapolate potential outcomes of how the momentum strategies might perform under different market conditions (as mentioned) in 1 000 iterations of each condition. The simple return analysis undertaken in this research revealed that traditional momentum outperformed residual momentum both before and throughout the COVID period. In the risk-adjusted performance measures, traditional momentum outperformed at all four risk indicators during the 2020 COVID year. The statistical significance tests, which compared the strategies' mean returns to the benchmark, demonstrated no statistically significant difference in returns over the COVID year. Furthermore, when evaluating the strategies over a five-year period (2018-2022), the difference in mean returns was shown to be statistically insignificant. However, statistical significance in returns was shown in some individual years. The ARDL regression findings show that bull, bear, and volatility factors explain relatively little of the returns for both momentum strategies, which is consistent with previous research. The Monte Carlo simulation, using the bear variable, forecasted that traditional momentum would result in negative returns during market declines, but residual momentum would provide positive returns and surpass traditional momentum with a probability of 26%. When using the bull variable, the simulation discovered that both traditional and residual momentum strategies resulted in positive returns. However, the residual momentum strategy outperformed in terms of returns and had an 84% likelihood of outperforming the traditional momentum strategy across 1,000 iterations. Nevertheless, when the simulation included the volatility variable, it projected negative returns for residual momentum and positive returns for traditional momentum. Additionally, it estimated a 14% probability of residual momentum surpassing traditional momentum under volatile market circumstances. , Thesis (MCom) -- Faculty of Commerce, Economics and Economic History, 2024
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- Date Issued: 2024-10-11
Exploring a new model for science engagement through documentary video production
- Authors: Mchunu, Nontokozo Philile
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/465897 , vital:76666
- Description: This study presents a model for science engagement that incorporates journalistic approaches. This model emerges from a project that aimed to create engagement and communication around a genetically modified sugar cane variety, developed by the South African Sugarcane Research Institute (SASRI). In this project, we engaged with small scale growers in the North and South Coast region of KwaZulu-Natal. This study’s methodology is based in action research and an iterative approach to science communication and engagement. It draws and reflects on an action research cycle captured through video documentary as a way to effectively and thoroughly collect, analyse and interpret data and produce through the case study. Based on this action research process a model for science engagement is proposed and reflected on and interrogated by scientists and science communicators through a focus group engagement (see model here as part of this thesis): https://drive.google.com/file/d/14UD1qd4fPcqIZrkESq5v2wgJ1Y2Q7hON/view?usp=sharing. I reflect, using video, on this model and how it incorporates principles and techniques for public engagement drawn from different approaches to journalism and communication studies, I argue that science engagement can benefit from drawing from journalistic approaches to public engagement such as those emerging from development communication, public journalism and development journalism. The submission of this thesis includes various other videos as part of the overall thesis. , Thesis (MA) -- Faculty of Humanities, Journalism and Media Studies, 2024
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- Date Issued: 2024-10-11