Quantifying the training demands of an amateur women’s Rugby union team using Global Positioning System
- Authors: Mbane, Nomabhelu
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424289 , vital:72140
- Description: Rugby union is a dynamic sport that has grown to not only be played by men but by women too. The professionalisation of the sport that happened in 1995 developed the game immeasurably including the focus given to its scientific research. This is important as literature on the demands of the game has become vital in improving the performance of Rugby union players (Deutch et al., 1998; Kay and Gill, 2004; Kruger, 2012). Scientific evidence-based data on the physical and physiological demands is imperative in the planning and setting up of training regimes (Deutch et al., 1998; Kay and Gill, 2004; Kruger, 2012). Unfortunately, the growth in the participation of women’s Rugby union has not been matched with scientific evidence-based literature that seeks to improve the performance of this population of players. Therefore, due to the unavailability of match-play during the COVID-19 outbreak, this study aimed to quantify the physical and physiological demands of training sessions of amateur women’s Rugby union players from the Sarah Baartman District, South Africa using Global Positioning System (GPS). The training’ data collected was compared to previous literature, including studies that also focused on match-play. Furthermore, players were categorized into forwards and backs, allowing for the comparison between the two playing categories. An exploratory, descriptive, observational, and comparative research design was used for the current study. The physical and physiological demands of eighteen women Rugby union players (eight forwards and ten backs) aged between 18 – 36 years old were assessed during training (for a total of nine sessions). To inform the participants’ characteristics, the players’ demographic, and anthropometric characteristics (age, stature, and body mass) were obtained. Using GPS and Zephyr technology the players’ training physical (time spent in speed zones, maximum speed, total distance travelled, number of impacts in the various impact zones) and physiological (heart rate) demands were explored. Statistical analysis methods including descriptive, independent t-test, one-way and two-way ANOVA tests were employed to analyse the data and evaluate the differences between forwards and backs. The mean age and stature for all players was 25,44±5,6 years and 1,60±0,06 m respectively, with no significant differences found between backs and forwards. With regards to body mass, forwards weighed 82,0±9,4 kg while backs weighed significantly lighter at 58,0±5,8 kg. Significant differences (p < 0,05) were found for all players in the time spent in speed zones and the number of impacts in impact zones. When all forwards’ and backs’ physical and physiological demands were compared the results showed no significant differences. The average time spent in all six speed zones combined was 11,41 and 11,62 mins for forwards and backs, respectively. The maximum speeds reached by forwards (21,35±5,72 km/h) and backs (22,85±3,88 km/h) were similar. All players combined travelled an average total distance of 2,14±0,76 km. The total number of impacts in all six impact zones, totalled 116 impacts for forwards and 97 for backs. Heart rate responses recorded for forwards and backs were 139±15 bpm and 134±13 bpm. Based on the findings of this study, the training intensity of amateur women’s Rugby union was lower than the demands experienced in the training and match-play of previous research. It was also observed that there was no specificity with regards to the training programs allocated between forwards and backs of this study. Further research on amateur women’s Rugby union is required to characterise training and match-play demands of these players. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
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- Date Issued: 2023-10-13
Quantifying the work demands of municipal waste workers to determine physical workload and perception
- Authors: Mamabolo, Lerato
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424267 , vital:72138
- Description: Objective: This study aimed to quantify physical and perceptual workloads through the nature of the work demands of municipal waste workers. Waste workers investigated were waste collectors, street sweepers [or public cleansers], drivers and mechanics. Methods: 154 City of Tshwane municipal waste workers volunteered to participate in the study. Observation-based tools were used to measure physical workload namely, the RAMP 1 and 2, REBA and RULA, and the hand-held dynamometer was used for pushing and pulling tasks. Subjective perceptual questionnaires were used to measure Perceptual load namely the NASA TLX and the QWIQ. Results: Differences in physical workload were evident between the occupation groups. Round bin collection was the waste collection type pertaining to the highest work-related risk of musculoskeletal development. Public cleansers and flatbed truck collectors' results displayed lower task-related risks associated in relation to the other occupations. Significant differences were found between the groups, particularly between waste collectors and drivers [had high workload outputs] in relation to public cleansers [low workload] for both perceptual workload tools. A significant difference was found in the mental and physical demands, as well as the organisational constraints and quantitative workloads. Mechanics showed no significant differences between groups but had high levels of frustration. Conclusion: A relationship between physical and perceived workload intensity is evident. development of musculoskeletal diseases and injury shall incur if measures are not reinstated. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
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- Date Issued: 2023-10-13
The dissipation of marital assets pending a divorce order in South Africa: a proposal for reform
- Authors: Mahlangu, Busisiwe Hlophane Maria
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424130 , vital:72126
- Description: Dissipation of marital assets takes place where one spouses uses the marital assets with the intention to frustrate the equitable distribution at the dissolution of marriage. This kind of behaviour usually occurs when one spouse anticipates a divorce or when the final divorce order is pending. In this context then, this thesis aims to critically analyse the effectiveness of the legal remedies available to spouses who are negatively affected by the dissipation of marital assets in marriage in community of property and marriage out of community of property with accrual. In this thesis the question is asked: does the current law adequately protect one spouse from another spouse unfairly hiding or dissipating assets from their estate to prevent the equitable distribution of these assets to the other spouse on divorce? In order to answer this question, this thesis proceeds by examining each of the remedies in detail, looking specifically at the statutory remedies set out in the Matrimonial Property Act 88 of 1984, specifically those contained in ss 8, 15 and 20 of that Act. It also considers remedies found in the common law. Ultimately, the study finds that these remedies do not provide adequate protection to spouses affected by the dissipation of marital assets and looks at the particular reasons for this finding. The study then concludes by proposing reforms to provide better protection to affected spouses. These legal reforms include: amending the legislative framework, changing the determinative date of the accrual, legislating a requirement for full disclosure of assets in terms s 8(1) of the Matrimonial Property Act and, finally, advocating for a flexible interpretation of the requirements of s 20 of the Matrimonial Property Act and the common law anti-dissipation remedy. , Thesis (LLM) -- Faculty of Law, Law, 2023
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- Date Issued: 2023-10-13
The effectiveness of the Therapeutic Sandstory Method as a focused intervention with a child: a descriptive single case study
- Authors: Francis, Staci
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424973 , vital:72198
- Description: This study explores and describes a phenomenological case study, illustrating a postmodern constructionist orientation to sandtray work with a South African child. The Therapeutic Sandstory Method (TSM) involves the innovative integration of a variety of tenets when working with children– constructing sandworlds, storytelling, the reflection of stories and coherent therapeutic documents in therapy collectively facilitating the healing process. This novel method enables children to translate and communicate their own personal experiences and traumas into a concrete form through storytelling and metaphoric connections constructed in their sandworld. It is anticipated that the TSM is a brief yet feasible, child-centered therapeutic engagement designed to continue the therapeutic process outside of the therapy setting, making it ideally suited for children and adolescents from under-resourced communities. The results of the study revealed improved interpersonal functioning and emotional wellbeing after eight sessions. Contextualized narrative and social constructionist findings are discussed and recommendations made pertaining to future research and practice using TSM with children in therapy. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
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- Date Issued: 2023-10-13
The ergonomic assessment of two different pipette models and their effects on biomechanical, performance, and subjective outcomes
- Authors: Masoka, Bonolo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424278 , vital:72139
- Description: While laboratory workers are essential in many industries, they are also at a high risk of developing musculoskeletal disorders (MSDs). Manual pipetting has been identified as the leading cause of MSDs in laboratories because it exposes operators to high forces, repetition, static muscular loading, and awkward wrist postures for prolonged periods. The main contributors to the high risk associated with manual pipetting are the design of manual pipettes and how they are used. A laboratory-based study was conducted to assess and compare the biomechanical, subjective, and performance responses of an ergonomically designed pipette model to those of a traditional model. In a simulated laboratory workstation, 20 individuals performed 40 repetitions of pipetting tasks using each pipette model. Dependent variables consisted of muscle activity (of the Abductor Pollicis Brevis, Opponens Pollicis, Extensor pollicis longus, Abductor pollicis longus, Extensor pollicis brevis, and brachioradialis muscles), wrist postures, time to task completion, perceived muscular exertion (measured using the Borg RPE scale), perceived comfort and usability, and user preference. The results revealed that an ergonomic modification to pipette design has some effect on biomechanical and subjective outcomes. The ergonomic model significantly reduced muscular loading of the Opponens pollicis, and Abductor pollicis brevis muscles. The ergonomic model also reduced radial deviation and was associated with better subjective responses, while the time to task completion did not change. This study found that an ergonomic modification to pipette design can improve user comfort and well-being without compromising performance. Overall, the findings emphasize the importance of addressing pipette design to reduce the MSD risk associated with manual pipetting, and the necessity for a user-centred approach to tool design. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
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- Date Issued: 2023-10-13
The heterologous expression and in vitro biochemical characterization of the Hsp70 escort protein 1 and mitochondrial Hsp70 partner proteins of the Trypanosoma brucei parasite and humans
- Authors: Mahlalela, Maduma Ernst
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431832 , vital:72807 , DOI 10.21504/10962/431832
- Description: The 70 kDa family of heat shock proteins (Hsp70) plays a central role in the maintenance of cellular proteostasis, with paralogues occurring in all the major compartments of the eukaryotic cell. Hsp70s act in conjunction with proteins known as co-chaperones, as part of the larger molecular chaperone network. In the mitochondrion, Hsp70 (mtHsp70) is responsible for the import of proteins synthesized in the cytosol, protein folding in the matrix and the maintenance of the iron-sulphur cluster. In human cells mtHsp70 (HSPA9) is also referred to as mortalin, as the knockdown of the protein leads to cell mortality. Trypanosoma brucei is the causative agent of sleeping sickness in humans and nagana in animals. In the T. brucei parasite there are three identical mtHsp70 (TbmtHsp70) proteins that are produced, forming part of the Hsp70 machinery that is essential for parasite survival. In humans, the levels of HSPA9 are often elevated in non-communicable diseases such as cancer and neurodegeneration. Despite their vital cellular roles, mtHsp70s are characteristically prone to self-aggregation. The binding of the Hsp70 escort protein (Hep1) is required to prevent the aggregation of mtHsp70 proteins, enabling the proteins to function. In many non-communicable diseases, mtHsp70 and other molecular chaperones such as heat shock protein 90 (Hsp90) are being investigated as potential drug targets. Existing anti-trypanosomal drugs for treating sleeping sickness are toxic, having adverse side effects that are potentially lethal. Investigations into Hsp70s, and other molecular chaperones, form part of the research into the discovery of novel and efficacious therapeutics. This is the first study to characterise Hep1 and investigate its partnership with mtHsp70 in T. brucei. The overall aim of this study was to comparatively assess the T. brucei and human mtHsp70/Hep1 partnerships. The putative T. brucei Hep1 (TbHep1) orthologue was analysed in silico, and it was found to possess a zinc finger domain consisting of anti-parallel β-sheets that are characteristic of canonical Hep1 proteins, whilst the N-terminal domain was unstructured. Based on sequence analysis, the regions outside of the zinc finger domains lacked conservation. Despite the lack of sequence conservation, the N- and C-terminal regions of TbHep1 shared segments of similarity with Hep1 orthologues of other kinetoplastid and trypanosomal orthologues. The same held true for the N- and C-termini of human Hep1 (HsHep1) when compared to other Hep1 orthologues of mammalian origin. Biochemical analysis revealed TbmtHsp70 and HSPA9 to be prone to self-aggregation, which was reduced by co-expression with TbHep1 and HsHep1, respectively. Recently Hep1 proteins have been determined to be present in the cytosol. In this study, TbHep1 and HsHep1 also interacted with the cytosolic Hsp70s, HSPA1A and TbHsp70, by preventing their thermally induced aggregation and stimulating their ATPase activities. TbHep1 and HsHep1 also suppressed the thermally induced aggregation of the model substrates malate dehydrogenase and citrate synthase, independently of Hsp70. To date, only two Hep1 orthologues, HsHep1 and LbHep1, have been found to function in a similar manner to a J-protein co-chaperone by stimulating the ATPase activities of their partner mtHsp70 proteins. In this study, TbHep1 stimulated the ATPase activity of TbmtHsp70. HsHep1 also stimulated the ATPase activity of TbmtHsp70. However, the mechanism of action still needs to be determined. This study also explored the potential of the Hep1 orthologues to be functionally activated by oxidative stress, which is prevalent in mitochondria. The abilities of TbHep1 and HsHep1 to reduce the thermally induced aggregation of malate dehydrogenase were enhanced under oxidative conditions. Disrupting the function of Hep1 has been found to eventually lead to cell death, and given the critical role played by mtHsp70 in the cell, this partnership could be exploited as a potential drug target. In conclusion, this study demonstrated that TbHep1 and HsHep1 functionally interact with mtHsp70s, whilst also possessing independent chaperone activities that are also potentially influenced by the environmental redox state. , Thesis (PhD) -- Faculty of Science, Biotechnology Innovation Centre, 2023
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- Date Issued: 2023-10-13
Wildlife-vehicle collisions mitigation measures using road ecological data and deep learning
- Authors: Nandutu, Irene
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431907 , vital:72814
- Description: Access restricted. Expected release in 2025. , Thesis (PhD) -- Faculty of Science, Mathematics, 2023
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- Date Issued: 2023-10-13