Market potential and profitability of improved maize open pollinated varieties in the Eastern Cape, South Africa
- Authors: Sibanda, Melusi
- Date: 2012-04
- Subjects: Hybrid corn , Crops -- Effect of stress on
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24598 , vital:63211
- Description: Maize is one of the most important crops grown by smallholder farmers in Amatole and O.R. Tambo Districts of the Eastern Cape. For this study, there were 13 maize varieties that were evaluated extensively by on-farm trials in selected areas under dry land and irrigated conditions. Nine were newly introduced and improved Open Pollinated Varieties (OPVs), while four were locally grown varieties. Among the locally grown varieties, one was a hybrid-check, while the remaining three were improved OPVs. The objectives of this study were: to investigate the potential marketability of newly introduced maize OPVs, identify factors influencing farmers‟ demand of improved maize OPVs and assess their profitability when compared with hybrids. One hundred and thirty seven sample households were interviewed using a structured questionnaire. The data was analyzed using descriptive statistics (frequency, percentages), gross margins and regression analyses. Data for computing gross margins was taken from the on-farm trials. Results showed that the actual production of improved maize OPVs constituted 0percent in Zanyokwe Irrigation Scheme (ZIS) and 20percent of the farmers in the O.R. Tambo District. At Silwindlala Women‟s Project (SWP), the improved maize OPV (Sahara) was grown. The majority of the farmers in the study areas were growing competitor varieties (hybrids and/or in conjunction with landraces). Results show that, there is potential market for improved maize OPVs in the Eastern Cape. The majority of the farmers, 87percent and 92percent in Amatole (SWP and ZIS) and O.R. Tambo Districts respectively, indicated that they were willing to adopt the newly introduced maize OPVs. Results showed that the hybrid PAN 6479 variety in general performed better than improved maize OPVs whereas in some areas, the improved maize OPVs had better gross margins and gross profit margins than the hybrid variety. The indicated genotypes did not show specific adaptation to selected environments. The demand model that employed a multiple regression model was used and data was analyzed using Statistical Package for the Social Science (SPSS) computer software. Results of the multiple regression model showed that household socio-economic factors (extension contact, access to credit, availability of household income, proportion of land area under cultivation, proportion of land area under (hybrids and landrace) and perceptions on seed color) and an input market factor (availability of the improved maize OPV seeds on the local shops) were the most important factors influencing farmers' decisions to demanding the improved maize OPVs. Extension contact, access to credit, level of household income and proportion of land area under cultivation, positively influenced the demand for the improved maize OPVs whereas the unavailability of the improved maize OPV seeds on the local shops, proportion of land area under hybrids and landraces and perceptions on seed color negatively influenced the demand for the improved maize OPVs. , Thesis (MSc) -- Faculty of Science and Agriculture, 2012
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- Date Issued: 2012-04
An evaluation of stress tolerant open pollinated maize varieties in selected environments of the Eastern Cape Province, South Africa
- Authors: Chimonyo, Vimbayi G P https://orcid.org/0000-0001-9912-9848
- Date: 2011-11
- Subjects: Crops -- Effect of stress on
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24487 , vital:63045
- Description: The use of farmer acceptable, stress tolerant open pollinated maize varieties (OPVs) could be a strategy to help increase maize productivity for resource-poor farmers in the Eastern Cape (EC) Province. The current study investigated the following: a) participatory selection of newly introduced stress tolerant maize OPVs; b) characteristics of maize producing farmers, their production constraints and criteria for variety selections; c) multi-environment yield trials in which genotype and environment interactions (GEI) were investigated, and d) morphological diversity of newly introduced maize varieties. Nine newly introduced maize OPVs were evaluated in this study. These varieties were: ZM 305, ZM 423 ZM 501, ZM 525, Obatanpa, ZM 621, ZM 627, which were from the International Maize and Wheat Improvement Centre (CIMMYT), BR 993, and Comp 4 which from where the International Institute of Tropical Agriculture (IITA). Check varieties, Pan 6479 (a hybrid) and three locally grown OPVs (Okavango, Afric 1 and Nelson‘s Choice) were also included. Participatory variety selection (PVS) was conducted during the 2009/10 summer season to evaluate farmer acceptance of these newly introduced OPVs. The most preferred varieties farmers were Okavango, ZM 305 and ZM 501, and these varieties were not significantly different from the highest yielding variety within each site. Therefore, varieties like ZM 305 and ZM 501 could easily be adopted by farmers, and their use could result in yield improvements. To gather information on farmer characteristics, and perceptions on maize production constraints and maize selection criteria, focus group discussions and household surveys were conducted during the 2009/2010 and 2010/2011 seasons, respectively. Results indicated that, elderly farmers dominated the farming communities. Maize production was generally low, with 98percent of the farmers obtaining less than 1.6 t/ha. The most important constraints affecting maize production were extreme weather events (floods and drought), pests and diseases, and poor access to credit. The most preferred traits that made up farmer selection criteria were ear traits such as taste, long cobs, and big kernels. Other traits, such as, prolificacy, early maturity, retainability of seed and dark leaves, were village specific. Yield trials, assessing genotype and environment interactions, were conducted in eight sites during the 2009/10 and 2010/11 seasons. The genotypes exhibited non-significant crossover and non-crossover GEI over the environments. Okavango, the most stable variety, was generally low yielding (4.28 t/ha) than other stable varieties such as ZM 305, ZM 501, ZM 621 and ZM 423. The later varieties had significantly (p<0.05) higher yields of between 4.46 t/ha and 4.97 t/ha. The highest yielding varieties, Pan 6479 (5.29 t/ha) and ZM 525 (4.87 t/ha), showed specific adaptations to high potential environments, while BR 993 (4.07 t/ha) and Afric 1 (4.24 t/ha) were low yielding, unstable and specifically adapted to low potential environments. New varieties, therefore, exhibited both specific and wide adaptation. Qualitative and quantitative traits were evaluated to establish the morphological diversity of the 13 varieties. Ear height, plant height, days to 50percent anthesis and grain yield contributed the most to variety diversity. Cluster Analysis discriminated varieties into four main clusters. The first cluster consisted of four CIMMYT varieties that were short in height and early maturing (ZM 305, ZM 423, ZM 501 and ZM 525), while hybrid Pan 6479 was placed into cluster two. Nelson‘s Choice and Okavango were grouped into the third cluster, while tall and late maturing varieties, ZM 621, ZM 627, Obatanpa, BR 993, Comp 4 and Afric 1, were placed in the fourth cluster. The segregation of the newly introduced varieties into two distinct groups shows that these varieties can be recommended into more than on cropping system and agro-ecology. Differences in village agro-ecologies resulted in farmers selecting varieties differently. This diversity in agro-ecology also brought about variations on farmer perceptions in selection criteria and production constraints. Most of the new varieties were observed to be superior in yield performance when compared to local check OPVs, exhibiting either wide or specific adaptation. The study also demonstrated that, the study of morphological diversity can be used to suggest varieties to different environmental potentials and cropping systems. Multi-evaluation trials were able to give an insight on variety preferences and performance. These new varieties should, therefore, be introduced to selected farmers living in their respective environments on the basis of results obtained. However, varieties still need to be evaluated under farmer-managed conditions to determine whether they actually bring about yield improvement when compared with current varieties being used. , Thesis (MSc) -- Faculty of Science and Agriculture, 2011
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- Date Issued: 2011-11
Differential expression and regulation of sucrose transporters in rice (Orzya sativa L, cv Nipponbare) during environmental stress conditions
- Authors: Ibraheem, Omodele
- Date: 2011
- Subjects: Crops -- Effect of stress on , Plant molecular genetics , Gene expression , Sucrose , Rice
- Language: English
- Type: Thesis , Doctoral , PhD (Biochemistry)
- Identifier: vital:11249 , http://hdl.handle.net/10353/330 , Crops -- Effect of stress on , Plant molecular genetics , Gene expression , Sucrose , Rice
- Description: Plant productivity is greatly affected by environmental stresses such as drought, salinity and insect herbivory. Plants respond and adapt to these stresses by exhibiting physiological as well as biochemical changes at the cellular and molecular levels in order to survive. Expression of a variety of genes which encode numerous membrane transporters have been demonstrated to be induced by these stresses in a variety of plants. The nutritional status of plants is controlled by these transporters, which are regulated by the transcription of the corresponding genes. In spite of these adverse stress effects on agricultural yield, only a few studies have focused on gene transcriptional and translational regulation of membrane transporters during environmental stress situations. Rice, like other plants, contains a number of sucrose transporters encoded by a family of genes. However, detailed knowledge of their roles, localization and regulation during environmental stress conditions is lacking. Bioinformatic tools were used to identify putative cis-acting regulatory elements that may be involved in the regulation of rice and Arabidopsis thaliana sucrose transporters. The possible cis-acting regulatory elements were predicted by scanning genomic sequences 1.5 kbp upstream of the sucrose transporter genes translational start sites, using Plant CARE, PLACE and Genomatix Matinspector professional data bases. Several cis-acting regulatory elements that are associated with plant development, plant hormonal regulation and stress response were identified, and were present in varying frequencies within the 1.5 kbp of 5′ regulatory region. The putative cis-acting regulatory elements that possibly are involved in the expression and regulation of sucrose transporter gene families in rice and Arabidopsis thaliana during cellular development or environmental stress conditions were identified as: A-box, RY, CAT, Pyrimidine-box, Sucrose-box, ABRE, ARF, ERE, GARE, Me-JA, ARE, DRE, GA-motif, GATA, GT-1, MYC, MYB, W-box, and I-box. Expression analysis was used to elucidate the role of rice (Oryza sativa L. cv Nipponbare) sucrose transporter (OsSUT) genes during drought and salinity treatments of three week old rice plants ( at four leaf stage) over a 10 days. Among the five rice OsSUT genes identified, only OsSUT2 was observed to be progressively up-regulated during drought and salinity treatments, while OsSUT1, OsSUT4 and OsSUT5 were expressed at low levels, and OsSUT3 showed no detectable transcript expression. Sucrose transport will be essential to meet the cellular energy demands and also for osmoprotectant activities during drought and salinity stresses. It therefore indicates that OsSUT2 which facilitates transport of sucrose from photosynthetic cells will be III essential for rice plants to cope with drought and salinity stresses, and cultivars with a higher OsSUT2 expression should be able to tolerate these environmental stresses better. The role of OsSUT in assimilate transport during rusty plum aphids (Hysteroneura setariae; Thomas) infestation on the leaves of three week old rice (Orzya sativa L. cv Nipponbare) cultivar plants, over a time-course of 1 to 10 days of treatments, was also examined by combination of gene expression and β-glucuronidase (GUS) reporter gene analysis. Real Time PCR analysis of the five OsSUT genes revealed that the expression of OsSUT1 was progressively up-regulated during the course of aphid infestation. OsSUT2 and OsSUT4 expression were comparatively low in both the control and treated plants. OsSUT5 showed no clear difference in transcript expression in both control and treated plants, while no detectable transcript expression of OsSUT3 could be found. The up-regulation of OsSUT1 gene was verified at protein level by western blot analysis in both the control and treated plants. OsSUT1 protein expression was found to increase with time during aphid infestation. A similar trend was noticeable in the control plants, however at a lower expression level. These demonstrate that the cellular expression of OsSUT1is regulated by both developmental and environmental factors. OsSUT1-promoter:::GUS reporter gene expression was observed within the vascular parenchyma and/or companion cells associated with phloem sieve elements of the large and small bundles in the phloem tissues of the flag leaf blade regions where feeding aphids were confined, which progressively increased with time of infestation. It is suggested that OsSUT1 may primarily play an essential role in phloem transport of assimilate to wounded tissues from adjacent health tissues or may be involved in the retrieval of assimilate back into the phloem to minimize loss caused by the infestation. Some OsSUT1-promoter:::GUS expression was also found in the metaxylem at 10 days after infestation, which could signify a recovery system in which sucrose lost into the xylem as a result of aphids feeding are retrieved back into the phloem through the vascular parenchyma. This was supported by the exposure of cut ends of matured OsSUT1-promoter:::GUS rice plant leaf to 2% sucrose solution. OsSUT1-promoter:::GUS expression was observed within the protoxylem, xylem and phloem parenchyma tissues. This indicates that sucrose translocating within the xylem tissues are retrieved into the phloem via the OsSUT1 localized within the parenchyma tissues. In conclusion, the differential expression and regulation of rice (Orzya sativa L. cv Nipponbare) sucrose transporters as reported here suggest that OsSUT2 and OsSUT1 were constitutively expressed compared to other rice sucrose transporters during drought and salinity, and rusty plum aphids (Hysteroneura setariae; Thomas) infestation stresses respectively. Thus, the expression and regulation of the sucrose transporters could be related to the physiological and nutritional requirements of the cells during plant developmental or environmental stress state that allows their differential expression.
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- Date Issued: 2011