Combining DNA barcoding and morphology to identify larval fishes from the nearshore environment off the south-east coast of South Africa
- Authors: Somana, Zinzi Sinazo
- Date: 2020
- Subjects: Fishes -- Larvae -- South Africa -- Identification , Fishes -- Genetics -- Research -- Technique , Fishes -- South Africa -- Classification , Genetic markers
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/144605 , vital:38362
- Description: The early life history stages of most marine fish species are undescribed. The problem is, most of these fishes have pelagic larvae which are minute, delicate forms. Linking the larval stage to an adult counterpart is extremely challenging as larvae are morphologically different from the adults. Historically, larval fish identification relied solely on distinguishing morphological characteristics and meristic measurements, which has resulted in taxonomic confusion and misidentification. The introduction of the deoxyribonucleic acid (DNA) barcoding technique as an alternative approach has been successful in positively identifying larval fishes. The correct identification of larval specimens is the key to a better understanding of larval ecology, which underpins the success of any adult fish population. This study aimed to positively identify larval fishes of the south-east coast of South Africa using morphological characteristics and DNA barcoding. Larval and eggs specimens for this study were collected from the shallow nearshore waters of the south-east coast of South Africa. A total of 177 larval specimens were used for morphological analysis. Body shape, gut shape, pigmentation and morphometric measurements (such as body depth, preanal length and total body length) were used to identify each specimen to the family level. In addition, a fragment of mitochondrial cytochrome c oxidase subunit 1 gene (COI) was adopted for sequencing to identify larval fish specimens and fish eggs. Sequences generated from this study were compared to those in the Barcode of Life Database (BOLD). When there were no close matches to a sequence, the GenBank nucleic acid sequence database, maintained by the National Center for Biotechnology Information (NCBI), was used as an alternative. A total of 18 different families were identified through morphology. Seventy-seven of the 177 larval specimens were not subjected to morphological identification due to physical damage. The majority of larvae identified using morphological characteristics belonged to either the Sparidae, Tripterygiidae or Gobiesocidae fish families. Through DNA barcoding, 12 fish families, 16 genera and 18 different species were identified. Ten DNA barcodes (categorised as ‘no match’) from 10 different larval specimens were not identified through any of the online databases. Therefore, the 2% threshold value was used to identify members of the same species. The K2P genetic distance relationships were calculated among the no match sequences and downloaded probability matches from NCBI. This resulted in two unknown specimens assigned to the Blenniidae and Gobiidae. All other taxa were identified to species level, except specimens representing the Gobiidae and Tripterygiidae families. Based on the K2P genetic distances Gobiidae representatives were categorised as members of the Caffrogobius genus. Twenty-eight barcodes represented specimens from the Tripterygiidae. DNA barcode data from COI was analysed using the standard phylogenetic procedures in MEGA6 to examine relationships and differentiation among sequences. These could not be identified to the lowest taxonomic rank due to limited sequence data to compare them with. The sequence data from these specimens gave different results in the two online databases. BOLD results were to family level (Tripterygiidae) and NCBI to the species level (Clinidae: Pavoclinus profundus). Results in this study confirmed the efficiency of the DNA barcoding technique in species level identification of fish larvae. The evidence from genetic barcodes of the Tripterygiidae specimens, supported by morphological characteristics, suggests the need for thorough research to identify the individuals to the species level. The fact that this study identified taxonomically problematic Gobiidae and Tripterygiidae specimens suggests that studies similar to this may highlight additional diversity and help to resolve the taxonomy of other species in these families. However, the lack of reference sequence data from the adult specimens, and especially those with cryptic diversity, were both shortcomings for the positive identification of larvae. With that being said, it shows the necessity for more research to be conducted on barcoding of larvae in general as to accommodate all kinds of species from biodiversity to economic perspectives.
- Full Text:
- Authors: Somana, Zinzi Sinazo
- Date: 2020
- Subjects: Fishes -- Larvae -- South Africa -- Identification , Fishes -- Genetics -- Research -- Technique , Fishes -- South Africa -- Classification , Genetic markers
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/144605 , vital:38362
- Description: The early life history stages of most marine fish species are undescribed. The problem is, most of these fishes have pelagic larvae which are minute, delicate forms. Linking the larval stage to an adult counterpart is extremely challenging as larvae are morphologically different from the adults. Historically, larval fish identification relied solely on distinguishing morphological characteristics and meristic measurements, which has resulted in taxonomic confusion and misidentification. The introduction of the deoxyribonucleic acid (DNA) barcoding technique as an alternative approach has been successful in positively identifying larval fishes. The correct identification of larval specimens is the key to a better understanding of larval ecology, which underpins the success of any adult fish population. This study aimed to positively identify larval fishes of the south-east coast of South Africa using morphological characteristics and DNA barcoding. Larval and eggs specimens for this study were collected from the shallow nearshore waters of the south-east coast of South Africa. A total of 177 larval specimens were used for morphological analysis. Body shape, gut shape, pigmentation and morphometric measurements (such as body depth, preanal length and total body length) were used to identify each specimen to the family level. In addition, a fragment of mitochondrial cytochrome c oxidase subunit 1 gene (COI) was adopted for sequencing to identify larval fish specimens and fish eggs. Sequences generated from this study were compared to those in the Barcode of Life Database (BOLD). When there were no close matches to a sequence, the GenBank nucleic acid sequence database, maintained by the National Center for Biotechnology Information (NCBI), was used as an alternative. A total of 18 different families were identified through morphology. Seventy-seven of the 177 larval specimens were not subjected to morphological identification due to physical damage. The majority of larvae identified using morphological characteristics belonged to either the Sparidae, Tripterygiidae or Gobiesocidae fish families. Through DNA barcoding, 12 fish families, 16 genera and 18 different species were identified. Ten DNA barcodes (categorised as ‘no match’) from 10 different larval specimens were not identified through any of the online databases. Therefore, the 2% threshold value was used to identify members of the same species. The K2P genetic distance relationships were calculated among the no match sequences and downloaded probability matches from NCBI. This resulted in two unknown specimens assigned to the Blenniidae and Gobiidae. All other taxa were identified to species level, except specimens representing the Gobiidae and Tripterygiidae families. Based on the K2P genetic distances Gobiidae representatives were categorised as members of the Caffrogobius genus. Twenty-eight barcodes represented specimens from the Tripterygiidae. DNA barcode data from COI was analysed using the standard phylogenetic procedures in MEGA6 to examine relationships and differentiation among sequences. These could not be identified to the lowest taxonomic rank due to limited sequence data to compare them with. The sequence data from these specimens gave different results in the two online databases. BOLD results were to family level (Tripterygiidae) and NCBI to the species level (Clinidae: Pavoclinus profundus). Results in this study confirmed the efficiency of the DNA barcoding technique in species level identification of fish larvae. The evidence from genetic barcodes of the Tripterygiidae specimens, supported by morphological characteristics, suggests the need for thorough research to identify the individuals to the species level. The fact that this study identified taxonomically problematic Gobiidae and Tripterygiidae specimens suggests that studies similar to this may highlight additional diversity and help to resolve the taxonomy of other species in these families. However, the lack of reference sequence data from the adult specimens, and especially those with cryptic diversity, were both shortcomings for the positive identification of larvae. With that being said, it shows the necessity for more research to be conducted on barcoding of larvae in general as to accommodate all kinds of species from biodiversity to economic perspectives.
- Full Text:
Come listen quickly
- Authors: Gouws, Leigh-Anne
- Date: 2020
- Subjects: South African fiction (English) -- 21st century
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/141559 , vital:37985
- Description: Creative work portfolio.
- Full Text:
- Authors: Gouws, Leigh-Anne
- Date: 2020
- Subjects: South African fiction (English) -- 21st century
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/141559 , vital:37985
- Description: Creative work portfolio.
- Full Text:
Commonplaces
- Authors: Orsmond, Joseph Granger
- Date: 2020
- Subjects: South African fiction (English) -- 21st century
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/144061 , vital:38307
- Description: My thesis is a collection of short to medium length poems. All of the subject matter is sustained by reflections, anecdotes or stories. The pieces in the collection are concerned with experiences linked to the seemingly ordinary and mundane. In this regard, I am inspired by how Alan Ziegler (in “Tales of Teaching” and Love at First Sight) and Raymond Carver (All of Us: The collected Poems) find stories in subject matter which is so commonplace, that it is often ignored creatively. Likewise, the lyricism and modes of expression of William Carlos Williams, Federico García Lorca and Luis Cernuda have informed how I write and structure my poetry.
- Full Text:
- Authors: Orsmond, Joseph Granger
- Date: 2020
- Subjects: South African fiction (English) -- 21st century
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/144061 , vital:38307
- Description: My thesis is a collection of short to medium length poems. All of the subject matter is sustained by reflections, anecdotes or stories. The pieces in the collection are concerned with experiences linked to the seemingly ordinary and mundane. In this regard, I am inspired by how Alan Ziegler (in “Tales of Teaching” and Love at First Sight) and Raymond Carver (All of Us: The collected Poems) find stories in subject matter which is so commonplace, that it is often ignored creatively. Likewise, the lyricism and modes of expression of William Carlos Williams, Federico García Lorca and Luis Cernuda have informed how I write and structure my poetry.
- Full Text:
Computer aided approaches against Human African Trypanosomiasis
- Authors: Kimuda, Magambo Phillip
- Date: 2020
- Subjects: African trypanosomiasis , African trypanosomiasis -- Chemotherapy , Genomics , Macrophage migration inhibitory factor , Trypanosoma brucei , Pteridines , Tetrahydrofolate dehydrogenase , Adenylic acid , Molecular dynamics , Principal components analysis , Bioinformatics , Single nucleotide polymorphisms , Single Nucleotide Variants , Candidate Gene Association Study (CGAS)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/142542 , vital:38089
- Description: The thesis presented here is divided into two parts under a common theme that is the use of computer based tools, genomics, and in vitro experiments to develop innovative ways of tackling Human African Trypanosomiasis (HAT). Part I of this thesis focused on the human host genetic determinants while Part II focused on the discovery of novel chemotherapeutics against the parasite. Part I is further sub-divided into two parts: The first involves a Candidate Gene Association Study (CGAS) on an African population to identify genetic determinants associated with disease and/or susceptibility to HAT. The second involves studying the effects of missense Single Nucleotide Variants (SNVs) on protein structure, dynamics, and function using Macrophage Migration Inhibitory Factor (MIF) as a case study. Part II is also sub-divided into two parts: The first involves a computer based rational drug discovery of potential inhibitors against the Trypanosoma the folate pathway; particularly by targeting Trypanosoma brucei Pteridine Reductase (TbPTR1) which is an enzyme used by trypanosomes to overcome T. brucei Dihydrofolate Reductase (TbDHFR) inhibition. Lastly the derivation of CHARMM force-field parameters that can be used to accurately model the geometry and dynamics of the T. brucei Phosphodiesterase B1 enzyme (TbrPDEB1) bimetallic active site center. The derived parameters were then used in MD simulations to characterise protein-ligand residue interactions that are important in TbrPDEB1 inhibition with the goal of targeting the cyclic Adenosine Monophosphate (cAMP) signalling pathway. In the CGAS we were unable to detect any genetic associations in the Ugandan cohort analysed that passed correction for multiple testing in spite of the study being sufficiently powered. Additionally, our study found no association of the Apo lipoprotein 1 (APOL1) G2 allele association with protection against acute HAT that has been previously reported. Future investigations for example, Genome Wide Association Studies using larger samples sizes (>3000 cases and controls) are required. Macrophage migration inhibitory factor (MIF) is a cytokine that is important in both innate and adaptive immunity that has been shown to play a role in T. brucei pathogenicity using murine models. A total of 27 missense SNVs were modelled using homology modelling to create MIF protein mutants that were investigated using in silico effect prediction tools, molecular dynamics (MD), Principal Component Analysis (PCA), and Dynamic Residue Network (DRN) analysis. Our results demonstrate that mutations P2Q, I5M, P16Q, L23F, T24S, T31I, Y37H, H41P, M48V, P44L, G52C, S54R, I65M, I68T, S75F, N106S, and T113S caused significant conformational changes. Further, DRN analysis showed that residues P2, T31, Y37, G52, I65, I68, S75, N106, and T113S are part of a similar local residue interaction network with functional significance. These results show how polymorphisms such as missense SNVs can affect protein conformation, dynamics, and function. Trypanosomes are auxotrophic for folates and pterins but require them for survival. They scavenge them from their hosts. PTR1 is a multifunctional enzyme that is unique to trypanosomatids that reduces both pterins and folates. In the presence of DHFR inhibitors, PTR1 is over-expressed thus providing an escape from the effects of DHFR inhibition. Both TbPTR1 and TbDHFR are pharmacologically and genetically validated drug targets. In this study 5742 compounds were screened using molecular docking, and 13 promising binding modes were further analysed using MD simulations. The trajectories were analysed using RMSD, Rg, RMSF, PCA, Essential Dynamics Analysis (EDA), Molecular Mechanics Poisson–Boltzmann surface area (MM-PBSA) binding free energy calculations, and DRN analysis. The computational screening approach allowed us to identify five of the compounds, named RUBi004, RUBi007, RUBi014, RUBi016 and RUBi018 that exhibited antitrypanosomal growth activities against trypanosomes in culture with IC50 values of 12.5 ± 4.8 μM, 32.4 ± 4.2 μM, 5.9 ± 1.4 μM, 28.2 ± 3.3 μM, and 9.7 ± 2.1 μM, respectively. Further when used in combination with WR99210 a known TbDHFR inhibitor RUBi004, RUBi007, RUBi014 and RUBi018 showed antagonism while RUBi016 showed an additive effect. These results indicate that the four compounds might be competing with TbDHFR while RUBi016 might be more specific for TbPTR1. These compounds provide scaffolds that can be further optimised to improve their potency and specificity. Lastly, using a systematic approach we derived CHARMM force-field parameters to accurately describe the TbrPDEB1 bi-metal catalytic center. For dynamics, we employed mixed bonded and non-bonded approach. We optimised the structure using a two-layer QM/MM ONIOM (B3LYP/6-31(g): UFF). The TbrPDEB1 bi-metallic center bonds, angles, and dihedrals were parameterized by fitting the energy profiles from Potential Energy Surface (PES) scans to the CHARMM potential energy function. The parameters were validated by means of MD simulations and analysed using RMSD, Rg, RMSF, hydrogen bonding, bond/angle/dihedral evaluations, EDA, PCA, and DRN analysis. The force-field parameters were able to accurately reproduce the geometry and dynamics of the TbrPDEB1 bi-metal catalytic center during MD simulations. Molecular docking was used to identify 6 potential hits, that inhibited trypanosome growth in vitro. The derived force-field parameters were used to simulate the 6 protein-ligand complexes with the aim of elucidating crucial protein-ligand residue interactions. Using the most potent ligand RUBi022 that had an IC50 of 14.96 μM we were able to identify key residue interactions that can be of use in in silico prediction of potential TbrPDEB1 inhibitors. Overall we demonstrate how bioinformatics tools can complement current disease eradication strategies. Future work will focus on identifying variants identified in Genome Wide Association Studies and partnering with wet labs to carry out further enzyme-ligand activity relationship studies, structure determination or characterisation of appropriate protein-ligand complexes by crystallography, and site specific mutation studies
- Full Text:
- Authors: Kimuda, Magambo Phillip
- Date: 2020
- Subjects: African trypanosomiasis , African trypanosomiasis -- Chemotherapy , Genomics , Macrophage migration inhibitory factor , Trypanosoma brucei , Pteridines , Tetrahydrofolate dehydrogenase , Adenylic acid , Molecular dynamics , Principal components analysis , Bioinformatics , Single nucleotide polymorphisms , Single Nucleotide Variants , Candidate Gene Association Study (CGAS)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/142542 , vital:38089
- Description: The thesis presented here is divided into two parts under a common theme that is the use of computer based tools, genomics, and in vitro experiments to develop innovative ways of tackling Human African Trypanosomiasis (HAT). Part I of this thesis focused on the human host genetic determinants while Part II focused on the discovery of novel chemotherapeutics against the parasite. Part I is further sub-divided into two parts: The first involves a Candidate Gene Association Study (CGAS) on an African population to identify genetic determinants associated with disease and/or susceptibility to HAT. The second involves studying the effects of missense Single Nucleotide Variants (SNVs) on protein structure, dynamics, and function using Macrophage Migration Inhibitory Factor (MIF) as a case study. Part II is also sub-divided into two parts: The first involves a computer based rational drug discovery of potential inhibitors against the Trypanosoma the folate pathway; particularly by targeting Trypanosoma brucei Pteridine Reductase (TbPTR1) which is an enzyme used by trypanosomes to overcome T. brucei Dihydrofolate Reductase (TbDHFR) inhibition. Lastly the derivation of CHARMM force-field parameters that can be used to accurately model the geometry and dynamics of the T. brucei Phosphodiesterase B1 enzyme (TbrPDEB1) bimetallic active site center. The derived parameters were then used in MD simulations to characterise protein-ligand residue interactions that are important in TbrPDEB1 inhibition with the goal of targeting the cyclic Adenosine Monophosphate (cAMP) signalling pathway. In the CGAS we were unable to detect any genetic associations in the Ugandan cohort analysed that passed correction for multiple testing in spite of the study being sufficiently powered. Additionally, our study found no association of the Apo lipoprotein 1 (APOL1) G2 allele association with protection against acute HAT that has been previously reported. Future investigations for example, Genome Wide Association Studies using larger samples sizes (>3000 cases and controls) are required. Macrophage migration inhibitory factor (MIF) is a cytokine that is important in both innate and adaptive immunity that has been shown to play a role in T. brucei pathogenicity using murine models. A total of 27 missense SNVs were modelled using homology modelling to create MIF protein mutants that were investigated using in silico effect prediction tools, molecular dynamics (MD), Principal Component Analysis (PCA), and Dynamic Residue Network (DRN) analysis. Our results demonstrate that mutations P2Q, I5M, P16Q, L23F, T24S, T31I, Y37H, H41P, M48V, P44L, G52C, S54R, I65M, I68T, S75F, N106S, and T113S caused significant conformational changes. Further, DRN analysis showed that residues P2, T31, Y37, G52, I65, I68, S75, N106, and T113S are part of a similar local residue interaction network with functional significance. These results show how polymorphisms such as missense SNVs can affect protein conformation, dynamics, and function. Trypanosomes are auxotrophic for folates and pterins but require them for survival. They scavenge them from their hosts. PTR1 is a multifunctional enzyme that is unique to trypanosomatids that reduces both pterins and folates. In the presence of DHFR inhibitors, PTR1 is over-expressed thus providing an escape from the effects of DHFR inhibition. Both TbPTR1 and TbDHFR are pharmacologically and genetically validated drug targets. In this study 5742 compounds were screened using molecular docking, and 13 promising binding modes were further analysed using MD simulations. The trajectories were analysed using RMSD, Rg, RMSF, PCA, Essential Dynamics Analysis (EDA), Molecular Mechanics Poisson–Boltzmann surface area (MM-PBSA) binding free energy calculations, and DRN analysis. The computational screening approach allowed us to identify five of the compounds, named RUBi004, RUBi007, RUBi014, RUBi016 and RUBi018 that exhibited antitrypanosomal growth activities against trypanosomes in culture with IC50 values of 12.5 ± 4.8 μM, 32.4 ± 4.2 μM, 5.9 ± 1.4 μM, 28.2 ± 3.3 μM, and 9.7 ± 2.1 μM, respectively. Further when used in combination with WR99210 a known TbDHFR inhibitor RUBi004, RUBi007, RUBi014 and RUBi018 showed antagonism while RUBi016 showed an additive effect. These results indicate that the four compounds might be competing with TbDHFR while RUBi016 might be more specific for TbPTR1. These compounds provide scaffolds that can be further optimised to improve their potency and specificity. Lastly, using a systematic approach we derived CHARMM force-field parameters to accurately describe the TbrPDEB1 bi-metal catalytic center. For dynamics, we employed mixed bonded and non-bonded approach. We optimised the structure using a two-layer QM/MM ONIOM (B3LYP/6-31(g): UFF). The TbrPDEB1 bi-metallic center bonds, angles, and dihedrals were parameterized by fitting the energy profiles from Potential Energy Surface (PES) scans to the CHARMM potential energy function. The parameters were validated by means of MD simulations and analysed using RMSD, Rg, RMSF, hydrogen bonding, bond/angle/dihedral evaluations, EDA, PCA, and DRN analysis. The force-field parameters were able to accurately reproduce the geometry and dynamics of the TbrPDEB1 bi-metal catalytic center during MD simulations. Molecular docking was used to identify 6 potential hits, that inhibited trypanosome growth in vitro. The derived force-field parameters were used to simulate the 6 protein-ligand complexes with the aim of elucidating crucial protein-ligand residue interactions. Using the most potent ligand RUBi022 that had an IC50 of 14.96 μM we were able to identify key residue interactions that can be of use in in silico prediction of potential TbrPDEB1 inhibitors. Overall we demonstrate how bioinformatics tools can complement current disease eradication strategies. Future work will focus on identifying variants identified in Genome Wide Association Studies and partnering with wet labs to carry out further enzyme-ligand activity relationship studies, structure determination or characterisation of appropriate protein-ligand complexes by crystallography, and site specific mutation studies
- Full Text:
Conceptualisations of and responses to plagiarism in the South African higher education system
- Mphahlele, Martha Matee (Amanda)
- Authors: Mphahlele, Martha Matee (Amanda)
- Date: 2020
- Subjects: Plagiarism , Plagiarism -- Prevention -- South Africa , Education, Higher -- Moral and ethical aspects , Education, Higher -- South Africa , Cheating (Education) -- South Africa , College students -- Legal status, laws, etc. -- South Africa , College discipline -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/162642 , vital:40963
- Description: Violations of academic integrity are a cause for concern in universities around the world and plagiarism is one of the most significant examples of these academic integrity issues with which universities are grappling . The approach taken to managing plagiarism depends to a large extent on the understanding of the phenomenon within institutions. This study investigated how plagiarism is conceptualised and responded to in the South African Higher Education system and how this impacts on teaching and learning. Data was collected from 25 out of the 26 South African public universities; the missing university had just been established and did not yet have policies or processes in place. The data was primarily in the form of documents known in these institutions as ‘plagiarism policies’, along with a wealth of other related policies and reports. This was supplemented by interviews as a means of verifying the document analysis with seven plagiarism committee members from across the three institutional types in South Africa, namely: traditional universities, comprehensive universities, and universities of technology. Using Bhaskar’s (2008) critical realism as a metatheory and Archer’s (1995) social realism as both a substantive theory and analytical framework, the experiences and events of plagiarism management were critically examined. Critical realism consider s these experiences and events at the level of the e mpirical and the actual , in order to identify the mechanisms at the l evel of the r eal from which these emerge. Social realism argues that when undertaking such an analysis in the social world, this entails identifying the emergent properties of both the parts (structure and culture) and people (agents). Therefore, the data was analysed using Archer’s analytical dualism to identify structural, cultural and agential mechanisms shaping the understanding of plagiarism and the practices associated with managing the phenomenon. The study found that dominant in the sector was an un derstanding of plagiarism as always being an intentional act, with implications for teaching and learning practices, which then focused on identifying and punishing incidents of plagiarism in student writing. A legal discourse was found to permeate the universities’ plagiarism management systems, such that most procedures replicated the legal framework. This was seen to undermine the identity of universities as teaching and learning spaces and of students as novice members of the disciplinary fields. The study further highlighted that due to plagiarism being perceived as an intentional act, punishment in almost all universities is prioritised as the key means of attending to plagiarism in the se institutions. This emerged as a structural constraint to students’ acquisition of academic writing norms. Such understandings and approaches were seen to be complementary to the risk-aversion of many institutions in a globalised era of university rankings. As increased bureaucracy has been put in place to attend to incidents of plagiarism, including obligatory reporting thereof, an unintentional consequence emerged, where it was at times simpler for academics to ignore incidences of plagiarism than to act on them. Turnitin was frequently referred to across the data as the preferred text - matching tool, but Turnitin together with other text-matching tools , was often used in a way that complemented the understanding of plagiarism as always being an intentional act. The stu dy found that text - matching software was largely misunderstood to be plagiarism software, where the similarity index was perceived to be a measure of plagiarism. This led to an understanding that students needed to paraphrase texts in order to avoid detect ion by the program me, and this may inadvertently encourage plagiarism , as students are taught to write towards the software. The research found that in those instances where educational responses to plagiarism were in place, they often demonstrated a lack of understanding of academic literacies development and the extent to which norms of knowledge production are disciplinary specific. Most (but not all) of the data about educational responses focused on add-on workshops and the signing of a declaration form, indicating that the student has not plagiarised. The workshops were seen to emphasise technical skills, such as the punctuation norms of referencing, and were often offered in a generic format by people outside of the target disciplines. These workshops were found to ignore the connection between the technical skills of referencing and the norms of knowledge construction, with a potential deleterious effect on the development of authorial identity. Finally, the data showed a few instances where particular institutions acknowledged that plagiarism occurs along a continuum, where on one side is intentional plagiarism associated with cheating and requiring punishment, and on the other side is unintentional plagiarism, which is understood to require an educational response , and was seen to emerge from either a lack of understanding of academic literacy norms , or from negligence. Literacy development with regard to taking on the norms of knowledge-making in the academy was seen to be a complex and lengthy process that was fundamental to educational endeavours of facilitating epistemological access, while cases of negligence were seen to be mainly caused by technical oversight rather than a lack of access to the relevant knowledge production norms. The study concludes by arguing that cases of intentional plagiarism require quick and appropriate punishment, but that there also needs to be an institution-wide understanding that unintentional plagiarism often emerges from students failing to access the specific knowledge-making norms of the discipline. There is thus a need for academics to be aware of the complexities related to taking on literacy practices, and who also understand the role of feedback in this process. But it ought not to be assumed that academics would have such insights simply by virtue of their expertise in the discipline. These academics need to have carefully constructed staff development support, as they take on such pedagogical approaches. The study argues that the dominant conceptualisation of plagiarism in the domain of culture as an intentional act and the complementary policies and processes in the domain of structure as focusing on detecting and punishing incidents of plagiarism, fail to address plagiarism in appropriate educational ways.
- Full Text:
- Authors: Mphahlele, Martha Matee (Amanda)
- Date: 2020
- Subjects: Plagiarism , Plagiarism -- Prevention -- South Africa , Education, Higher -- Moral and ethical aspects , Education, Higher -- South Africa , Cheating (Education) -- South Africa , College students -- Legal status, laws, etc. -- South Africa , College discipline -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/162642 , vital:40963
- Description: Violations of academic integrity are a cause for concern in universities around the world and plagiarism is one of the most significant examples of these academic integrity issues with which universities are grappling . The approach taken to managing plagiarism depends to a large extent on the understanding of the phenomenon within institutions. This study investigated how plagiarism is conceptualised and responded to in the South African Higher Education system and how this impacts on teaching and learning. Data was collected from 25 out of the 26 South African public universities; the missing university had just been established and did not yet have policies or processes in place. The data was primarily in the form of documents known in these institutions as ‘plagiarism policies’, along with a wealth of other related policies and reports. This was supplemented by interviews as a means of verifying the document analysis with seven plagiarism committee members from across the three institutional types in South Africa, namely: traditional universities, comprehensive universities, and universities of technology. Using Bhaskar’s (2008) critical realism as a metatheory and Archer’s (1995) social realism as both a substantive theory and analytical framework, the experiences and events of plagiarism management were critically examined. Critical realism consider s these experiences and events at the level of the e mpirical and the actual , in order to identify the mechanisms at the l evel of the r eal from which these emerge. Social realism argues that when undertaking such an analysis in the social world, this entails identifying the emergent properties of both the parts (structure and culture) and people (agents). Therefore, the data was analysed using Archer’s analytical dualism to identify structural, cultural and agential mechanisms shaping the understanding of plagiarism and the practices associated with managing the phenomenon. The study found that dominant in the sector was an un derstanding of plagiarism as always being an intentional act, with implications for teaching and learning practices, which then focused on identifying and punishing incidents of plagiarism in student writing. A legal discourse was found to permeate the universities’ plagiarism management systems, such that most procedures replicated the legal framework. This was seen to undermine the identity of universities as teaching and learning spaces and of students as novice members of the disciplinary fields. The study further highlighted that due to plagiarism being perceived as an intentional act, punishment in almost all universities is prioritised as the key means of attending to plagiarism in the se institutions. This emerged as a structural constraint to students’ acquisition of academic writing norms. Such understandings and approaches were seen to be complementary to the risk-aversion of many institutions in a globalised era of university rankings. As increased bureaucracy has been put in place to attend to incidents of plagiarism, including obligatory reporting thereof, an unintentional consequence emerged, where it was at times simpler for academics to ignore incidences of plagiarism than to act on them. Turnitin was frequently referred to across the data as the preferred text - matching tool, but Turnitin together with other text-matching tools , was often used in a way that complemented the understanding of plagiarism as always being an intentional act. The stu dy found that text - matching software was largely misunderstood to be plagiarism software, where the similarity index was perceived to be a measure of plagiarism. This led to an understanding that students needed to paraphrase texts in order to avoid detect ion by the program me, and this may inadvertently encourage plagiarism , as students are taught to write towards the software. The research found that in those instances where educational responses to plagiarism were in place, they often demonstrated a lack of understanding of academic literacies development and the extent to which norms of knowledge production are disciplinary specific. Most (but not all) of the data about educational responses focused on add-on workshops and the signing of a declaration form, indicating that the student has not plagiarised. The workshops were seen to emphasise technical skills, such as the punctuation norms of referencing, and were often offered in a generic format by people outside of the target disciplines. These workshops were found to ignore the connection between the technical skills of referencing and the norms of knowledge construction, with a potential deleterious effect on the development of authorial identity. Finally, the data showed a few instances where particular institutions acknowledged that plagiarism occurs along a continuum, where on one side is intentional plagiarism associated with cheating and requiring punishment, and on the other side is unintentional plagiarism, which is understood to require an educational response , and was seen to emerge from either a lack of understanding of academic literacy norms , or from negligence. Literacy development with regard to taking on the norms of knowledge-making in the academy was seen to be a complex and lengthy process that was fundamental to educational endeavours of facilitating epistemological access, while cases of negligence were seen to be mainly caused by technical oversight rather than a lack of access to the relevant knowledge production norms. The study concludes by arguing that cases of intentional plagiarism require quick and appropriate punishment, but that there also needs to be an institution-wide understanding that unintentional plagiarism often emerges from students failing to access the specific knowledge-making norms of the discipline. There is thus a need for academics to be aware of the complexities related to taking on literacy practices, and who also understand the role of feedback in this process. But it ought not to be assumed that academics would have such insights simply by virtue of their expertise in the discipline. These academics need to have carefully constructed staff development support, as they take on such pedagogical approaches. The study argues that the dominant conceptualisation of plagiarism in the domain of culture as an intentional act and the complementary policies and processes in the domain of structure as focusing on detecting and punishing incidents of plagiarism, fail to address plagiarism in appropriate educational ways.
- Full Text:
Conceptualising community radio: from global histories to the Kenyan example
- Authors: Korir, Geoffrey Kiplimo
- Date: 2020
- Subjects: Community radio , Community radio -- Kenya , Community development -- Kenya , Social change -- Kenya , Radio broadcasting -- Kenya
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/165766 , vital:41279
- Description: This study sets out to gain insight into the way that the establishment of community radio in Kenya is located within the global history of ideas about such radio. The first four chapters in this study provide terms of reference for this examination through a series of literature reviews. It sets out, firstly, to locate the internationally shared conceptualisaiton of community radio within an existing spectrum of approaches to social analysis. It proposes that community radio can be located at the critical, emancipatory end of this spectrum. It then maps out the global circulation of ideas about community radio and proposes that such circulation was informed by the broader history of critical, emancipatory social analysis. The final literature review then deals with community radio in Kenya and examines the way in which the establishment of this sector was shaped by the social and political history of this country. It is argued that the establishment of a community radio sector became possible only when support for emancipatory approaches to media became acceptable in Kenya, in context of the establishment of multiparty democracy. It is proposed, further, that the articulation of a vision for community radio in Kenya depended in part on the existence of international support for such radio and in part on the efforts of local actors in civil society. The empirical component then focuses on the way global ideas about community radio have become realised in Kenya. The study achieves this purpose by drawing on qualitative interviews with individuals from within civil society who have participated in the history of the establishment and growth of community radio in Kenya. These individuals demonstrate consciousness of the internationally shared set of principles that can facilitate a successful establishment of community radio. However, they are also sceptical of the assumption that guidelines for community radio are universally applicable. They point, in particular, to the challenges involved in the realisation of these guidelines in an environment in which economic resources are limited, and which is characterised by extreme social inequality and conflict. The study concludes that it is individuals such as these participants, who are embedded within the local context, who are best placed to articulate locally appropriate alternatives to these guidelines.
- Full Text:
- Authors: Korir, Geoffrey Kiplimo
- Date: 2020
- Subjects: Community radio , Community radio -- Kenya , Community development -- Kenya , Social change -- Kenya , Radio broadcasting -- Kenya
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/165766 , vital:41279
- Description: This study sets out to gain insight into the way that the establishment of community radio in Kenya is located within the global history of ideas about such radio. The first four chapters in this study provide terms of reference for this examination through a series of literature reviews. It sets out, firstly, to locate the internationally shared conceptualisaiton of community radio within an existing spectrum of approaches to social analysis. It proposes that community radio can be located at the critical, emancipatory end of this spectrum. It then maps out the global circulation of ideas about community radio and proposes that such circulation was informed by the broader history of critical, emancipatory social analysis. The final literature review then deals with community radio in Kenya and examines the way in which the establishment of this sector was shaped by the social and political history of this country. It is argued that the establishment of a community radio sector became possible only when support for emancipatory approaches to media became acceptable in Kenya, in context of the establishment of multiparty democracy. It is proposed, further, that the articulation of a vision for community radio in Kenya depended in part on the existence of international support for such radio and in part on the efforts of local actors in civil society. The empirical component then focuses on the way global ideas about community radio have become realised in Kenya. The study achieves this purpose by drawing on qualitative interviews with individuals from within civil society who have participated in the history of the establishment and growth of community radio in Kenya. These individuals demonstrate consciousness of the internationally shared set of principles that can facilitate a successful establishment of community radio. However, they are also sceptical of the assumption that guidelines for community radio are universally applicable. They point, in particular, to the challenges involved in the realisation of these guidelines in an environment in which economic resources are limited, and which is characterised by extreme social inequality and conflict. The study concludes that it is individuals such as these participants, who are embedded within the local context, who are best placed to articulate locally appropriate alternatives to these guidelines.
- Full Text:
Conserving land for people: transformative adaptive co-management of sustainable protected areas in the eastern Democratic Republic of Congo
- Authors: Kalyongo, Kujirakwinja Deo
- Date: 2020
- Subjects: Protected areas -- Congo (Democratic Republic) , Protected areas -- Government policy -- Congo (Democratic Republic) , Protected areas -- Management -- Congo (Democratic Republic) , Conservation of natural resources -- Congo (Democratic Republic)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/165912 , vital:41296
- Description: Conservation practices and approaches in the Democratic Republic of Congo (DRC), as in other parts of the world, have evolved from traditional to fortress and collaborative contemporary approaches such as adaptive co-management. These approaches aim to include multiple decision-makers using diversified resources and, existing formal and informal governance structures. Collaborative approaches also consider conflict resolution and external factors that can influence conservation outputs and outcomes. In the DRC context, conflicts over resources are related not only to resource access and power but also to the ineffectiveness of collaborative approaches that exclude stakeholders such as local communities. These conflicts have negatively affected protected areas and weakened the management and governance of protected areas. Therefore, evolving approaches such as adaptive co-management that consider power relations, the multi-scaled involvement of actors and learning loops to adjust strategies are seen as better options to improve the governance of protected areas and minimise the degradation of key ecosystems. My research explored the gazettement processes of three protected areas in the eastern DRC (Itombwe, Kabobo and Okapi Reserves). I focused on the influence of social-political, historical and psychological factors on the management and governance of protected areas in the DRC. In addition, the research reveals the inclusive gazettement processes of protected areas is the foundation of successful co-management approaches. I found that values and good governance practices play a key role in influencing local perceptions and support to conservation interventions. Whilst some conservation practitioners believe that economic benefits to communities are the most predominant motivating factor, I found that good conservation management practices can motivate communities to support protected area management. Bad management practices were related not only to inadequate conservation approaches and practices but also to factors such as corruption, inadequate law enforcement and the inappropriate equipment of rangers. Therefore, I suggest that long term protected area management in DRC should consider how the value of resources for communities and protected areas have been changing throughout the history of conservation, and how to best share power and responsibilities with local resource users and stakeholders. This is only possible if conservation practices and approaches, governance process and institutions are transformed at multiple levels.
- Full Text:
- Authors: Kalyongo, Kujirakwinja Deo
- Date: 2020
- Subjects: Protected areas -- Congo (Democratic Republic) , Protected areas -- Government policy -- Congo (Democratic Republic) , Protected areas -- Management -- Congo (Democratic Republic) , Conservation of natural resources -- Congo (Democratic Republic)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/165912 , vital:41296
- Description: Conservation practices and approaches in the Democratic Republic of Congo (DRC), as in other parts of the world, have evolved from traditional to fortress and collaborative contemporary approaches such as adaptive co-management. These approaches aim to include multiple decision-makers using diversified resources and, existing formal and informal governance structures. Collaborative approaches also consider conflict resolution and external factors that can influence conservation outputs and outcomes. In the DRC context, conflicts over resources are related not only to resource access and power but also to the ineffectiveness of collaborative approaches that exclude stakeholders such as local communities. These conflicts have negatively affected protected areas and weakened the management and governance of protected areas. Therefore, evolving approaches such as adaptive co-management that consider power relations, the multi-scaled involvement of actors and learning loops to adjust strategies are seen as better options to improve the governance of protected areas and minimise the degradation of key ecosystems. My research explored the gazettement processes of three protected areas in the eastern DRC (Itombwe, Kabobo and Okapi Reserves). I focused on the influence of social-political, historical and psychological factors on the management and governance of protected areas in the DRC. In addition, the research reveals the inclusive gazettement processes of protected areas is the foundation of successful co-management approaches. I found that values and good governance practices play a key role in influencing local perceptions and support to conservation interventions. Whilst some conservation practitioners believe that economic benefits to communities are the most predominant motivating factor, I found that good conservation management practices can motivate communities to support protected area management. Bad management practices were related not only to inadequate conservation approaches and practices but also to factors such as corruption, inadequate law enforcement and the inappropriate equipment of rangers. Therefore, I suggest that long term protected area management in DRC should consider how the value of resources for communities and protected areas have been changing throughout the history of conservation, and how to best share power and responsibilities with local resource users and stakeholders. This is only possible if conservation practices and approaches, governance process and institutions are transformed at multiple levels.
- Full Text:
Constructions of United States government development funding in response to the global gag rule
- Molobela, Reabetswe Lee-Anne
- Authors: Molobela, Reabetswe Lee-Anne
- Date: 2020
- Subjects: Pro-choice movement -- South Africa , Abortion -- Law and legislation -- South Africa , Abortion -- Moral and ethical aspects -- South Africa , Abortion -- Psychological aspects , Reproductive rights -- South Africa , Economic assistance, American -- South Africa , Non-governmental organizations -- South Africa , Discourse analysis , Social constructionism , Global Gag Rule
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/148295 , vital:38727
- Description: Despite South Africa’s progressive abortion law, barriers to safe abortion are numerous and exist at both the macro and micro level. Barriers include abortion stigma, discrimination, strong moral judgements against abortion within society and conscientious objection among health care workers. Furthermore, women’s lack of knowledge regarding the legal status of abortion and the voluminous illegal advertisements of back street abortions undermines the legislation and promotes unsafe abortions. Sexual and reproductive health rights (SRHR) non-governmental organizations (NGOs) have served as a link between service and people by providing information about safe abortion to women, especially in rural areas and have received funding from various platforms including United States government. However, the United States government has established the global gag rule which forbids foreign non-governmental organizations receiving United States government funding from using United States government and non-United States funds for abortion related activities. The global gag rule has been reinstated and extended by the current United States president. As such the global gag rule is expected to have an adverse effect on sexual and reproductive health rights in South Africa and on Sexual and reproductive health rights non-governmental organizations. The aim of the study is to highlight the constructions and responses to the global gag rule by sexual and reproductive health rights non-government organization workers in the South African context. This study used semi-structured individual interviews to collect data through purposive and snowball sampling of 10 South African Sexual and reproductive health rights non-governmental organizations workers. The study is situated within the social constructionist framework with emphasis on Fairclough’s three aspects of the constructive effects of discourse as an analytic tool in conjunction with Braun and Clarke’s social constructionist thematic analysis. The results of the study reflect on participants’ construction of United States government as imposing conservative agendas and taking regressive steps towards Sexual and reproductive health rights, which have in turn invoked indirect and direct resistance from non-governmental organizations. Additionally, NGO workers have constructed subject positions that highlight the vulnerability of non-governmental organizations dependency on United States government 1funding as it destabilizes and fragments civil society organization while it compromises the effectiveness of non-governmental organizations in serving the needs of intended communities. United States government is also constructed as strengthening abortion stigma and strengthening barriers to safe abortion that already exist in the country.
- Full Text:
- Authors: Molobela, Reabetswe Lee-Anne
- Date: 2020
- Subjects: Pro-choice movement -- South Africa , Abortion -- Law and legislation -- South Africa , Abortion -- Moral and ethical aspects -- South Africa , Abortion -- Psychological aspects , Reproductive rights -- South Africa , Economic assistance, American -- South Africa , Non-governmental organizations -- South Africa , Discourse analysis , Social constructionism , Global Gag Rule
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/148295 , vital:38727
- Description: Despite South Africa’s progressive abortion law, barriers to safe abortion are numerous and exist at both the macro and micro level. Barriers include abortion stigma, discrimination, strong moral judgements against abortion within society and conscientious objection among health care workers. Furthermore, women’s lack of knowledge regarding the legal status of abortion and the voluminous illegal advertisements of back street abortions undermines the legislation and promotes unsafe abortions. Sexual and reproductive health rights (SRHR) non-governmental organizations (NGOs) have served as a link between service and people by providing information about safe abortion to women, especially in rural areas and have received funding from various platforms including United States government. However, the United States government has established the global gag rule which forbids foreign non-governmental organizations receiving United States government funding from using United States government and non-United States funds for abortion related activities. The global gag rule has been reinstated and extended by the current United States president. As such the global gag rule is expected to have an adverse effect on sexual and reproductive health rights in South Africa and on Sexual and reproductive health rights non-governmental organizations. The aim of the study is to highlight the constructions and responses to the global gag rule by sexual and reproductive health rights non-government organization workers in the South African context. This study used semi-structured individual interviews to collect data through purposive and snowball sampling of 10 South African Sexual and reproductive health rights non-governmental organizations workers. The study is situated within the social constructionist framework with emphasis on Fairclough’s three aspects of the constructive effects of discourse as an analytic tool in conjunction with Braun and Clarke’s social constructionist thematic analysis. The results of the study reflect on participants’ construction of United States government as imposing conservative agendas and taking regressive steps towards Sexual and reproductive health rights, which have in turn invoked indirect and direct resistance from non-governmental organizations. Additionally, NGO workers have constructed subject positions that highlight the vulnerability of non-governmental organizations dependency on United States government 1funding as it destabilizes and fragments civil society organization while it compromises the effectiveness of non-governmental organizations in serving the needs of intended communities. United States government is also constructed as strengthening abortion stigma and strengthening barriers to safe abortion that already exist in the country.
- Full Text:
Creative production and existential thought: a feminist existential analysis of South African visual artist Berni Searle’s artwork
- Authors: Mokwena, Palesa
- Date: 2020
- Subjects: Women artists -- South Africa , Women artists, Black -- South Africa , Existentialism and art , Feminism and art -- South Africa , Searle, Berni
- Language: English
- Type: text , Thesis , Masters , MFA
- Identifier: http://hdl.handle.net/10962/148005 , vital:38701
- Description: Through an analysis of the work of South African artist Berni Searle, my study will investigate feminist existential ideas and concepts that have been explored by various creative producers in and outside of Europe/South Africa through different forms of creative productions and under different epistemological categories. ‘Canons’ of European existentialist/feminist thought often exclude the existence of feminist existential knowledge productions and producers outside of Europe. In conducting this study, I am responding to the past and present separatist and identitarian categorisations of creative productions from black/African creative producers, particularly women creative producers in South Africa, creating an alternative canonisation around their selected works. Although canons have been and can be used to drive separatist and identitarian categorisations, it is my hope to elucidate a discourse around the preservations and acknowledgements of South African creative and knowledge productions through a feminist existential framework that canonises important black feminist existentialist works and thereby brings to light their intellectual contributions over and above their identities. My development of a South African feminist existentialism is an attempt to graft a more intersectional, holistic framework to introduce in the feminist and existential discourses, and to proffer a new intersectional holistic paradigm of discussing categories that do not limit creative productions. To frame this research, I will reflect on the politics of historical and contemporary South African society as it is reflected in the works of the chosen creative producers and theorists and to question how we respond as creative feminist existentialists to contemporary South African struggles and how such a lens can be activated as a creative-theoretical tool of investigation.
- Full Text:
- Authors: Mokwena, Palesa
- Date: 2020
- Subjects: Women artists -- South Africa , Women artists, Black -- South Africa , Existentialism and art , Feminism and art -- South Africa , Searle, Berni
- Language: English
- Type: text , Thesis , Masters , MFA
- Identifier: http://hdl.handle.net/10962/148005 , vital:38701
- Description: Through an analysis of the work of South African artist Berni Searle, my study will investigate feminist existential ideas and concepts that have been explored by various creative producers in and outside of Europe/South Africa through different forms of creative productions and under different epistemological categories. ‘Canons’ of European existentialist/feminist thought often exclude the existence of feminist existential knowledge productions and producers outside of Europe. In conducting this study, I am responding to the past and present separatist and identitarian categorisations of creative productions from black/African creative producers, particularly women creative producers in South Africa, creating an alternative canonisation around their selected works. Although canons have been and can be used to drive separatist and identitarian categorisations, it is my hope to elucidate a discourse around the preservations and acknowledgements of South African creative and knowledge productions through a feminist existential framework that canonises important black feminist existentialist works and thereby brings to light their intellectual contributions over and above their identities. My development of a South African feminist existentialism is an attempt to graft a more intersectional, holistic framework to introduce in the feminist and existential discourses, and to proffer a new intersectional holistic paradigm of discussing categories that do not limit creative productions. To frame this research, I will reflect on the politics of historical and contemporary South African society as it is reflected in the works of the chosen creative producers and theorists and to question how we respond as creative feminist existentialists to contemporary South African struggles and how such a lens can be activated as a creative-theoretical tool of investigation.
- Full Text:
Critical success factors in implementing projects on restituted land parcels in South Africa
- Authors: Whiting, Christoper Whiting
- Date: 2020
- Subjects: Community development -- Management -- South Africa , Community development -- Management -- South Africa -- Case studies , Economic development projects -- Management -- South Africa , Economic development projects -- Management -- South Africa -- Case studies , Land reform -- South Africa -- Economic aspects , Land reform beneficiaries -- South Africa -- Case studies , Restitution -- South Africa -- Case studies
- Language: English
- Type: text , Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10962/167697 , vital:41504
- Description: Currently there is a perception in South Africa that projects undertaken on parcels of restituted land are experiencing a much higher failure than success rate. There are, however, a few projects that are reaping the rewards and uplifting their communities, as intended by the land redistribution program. One community in particular, located in Mpumalanga, called community A, is presently undertaking a development project. This development project is one of the most successful restituted land parcel projects ever in South Africa. The purpose of the study is to determine the critical success factors in this community development project that have allowed it to enjoy such high levels of success. Are these critical success factors different from the generic industry critical success factors and, if so, what are they? As informed through literature, the critical success factors focused on in this study are as follows: the influence of management, project communication, knowledge management, the role of senior management, business strategy, project success measurements, and training and development. For the empirical study, a physical questionnaire was handed to 35 management members currently employed in the community development project. The questionnaire requested the demographics of all participants and asked them to rate, using a five-point Likert scale, their perceptions of the present project activities and of the importance of the project activities mentioned in making the project successful. Participants had to indicate the extent to which they agreed with each statement in the questionnaire. The responses obtained from the survey were presented and analysed. The study found that the critical success factor “role of senior management” was the largest problem area in the restituted land development project. “Training and development opportunities” came in second, with both factor areas showing large variances. Recommendations have been given on how to address both these problematic areas in the development project and how future restituted land parcel projects can better utilize these factors to ensure successful project implementation and long-term project success.
- Full Text:
- Authors: Whiting, Christoper Whiting
- Date: 2020
- Subjects: Community development -- Management -- South Africa , Community development -- Management -- South Africa -- Case studies , Economic development projects -- Management -- South Africa , Economic development projects -- Management -- South Africa -- Case studies , Land reform -- South Africa -- Economic aspects , Land reform beneficiaries -- South Africa -- Case studies , Restitution -- South Africa -- Case studies
- Language: English
- Type: text , Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10962/167697 , vital:41504
- Description: Currently there is a perception in South Africa that projects undertaken on parcels of restituted land are experiencing a much higher failure than success rate. There are, however, a few projects that are reaping the rewards and uplifting their communities, as intended by the land redistribution program. One community in particular, located in Mpumalanga, called community A, is presently undertaking a development project. This development project is one of the most successful restituted land parcel projects ever in South Africa. The purpose of the study is to determine the critical success factors in this community development project that have allowed it to enjoy such high levels of success. Are these critical success factors different from the generic industry critical success factors and, if so, what are they? As informed through literature, the critical success factors focused on in this study are as follows: the influence of management, project communication, knowledge management, the role of senior management, business strategy, project success measurements, and training and development. For the empirical study, a physical questionnaire was handed to 35 management members currently employed in the community development project. The questionnaire requested the demographics of all participants and asked them to rate, using a five-point Likert scale, their perceptions of the present project activities and of the importance of the project activities mentioned in making the project successful. Participants had to indicate the extent to which they agreed with each statement in the questionnaire. The responses obtained from the survey were presented and analysed. The study found that the critical success factor “role of senior management” was the largest problem area in the restituted land development project. “Training and development opportunities” came in second, with both factor areas showing large variances. Recommendations have been given on how to address both these problematic areas in the development project and how future restituted land parcel projects can better utilize these factors to ensure successful project implementation and long-term project success.
- Full Text:
Crossing shades
- Authors: Singh, Shareen
- Date: 2020
- Subjects: South African fiction (English) -- 21st century
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/145049 , vital:38404
- Description: This collection of stories draw on culture, history, memory, musings and imagination. The stories are set primarily in South Africa but includes travels to other countries. I explore journeys to different worlds and minds. I challenge the reader to see how place and time influence our ways of seeing, living and evolving. I use different forms and tones that resonate with the subjective nature of each creative piece. My writing includes formal prose as well as works that experiment with fragments, vignettes and flash fiction.
- Full Text:
- Authors: Singh, Shareen
- Date: 2020
- Subjects: South African fiction (English) -- 21st century
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/145049 , vital:38404
- Description: This collection of stories draw on culture, history, memory, musings and imagination. The stories are set primarily in South Africa but includes travels to other countries. I explore journeys to different worlds and minds. I challenge the reader to see how place and time influence our ways of seeing, living and evolving. I use different forms and tones that resonate with the subjective nature of each creative piece. My writing includes formal prose as well as works that experiment with fragments, vignettes and flash fiction.
- Full Text:
Cyclooxygenase-1 as an anti-stroke target: potential inhibitor identification and non-synonymous single nucleotide polymorphism analysis
- Authors: Muronzi, Tendai
- Date: 2020
- Subjects: Cerebrovascular disease , Cerebrovascular disease -- Treatment , Cerebrovascular disease -- Chemotherapy , Cyclooxygenases , High throughput screening (Drug development) , Drug development , Molecular dynamics , South African Natural Compounds Database , ZINC database
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/143404 , vital:38243
- Description: Stroke is the third leading cause of death worldwide, with 87% of cases being ischemic stroke. The two primary therapeutic strategies to reduce post-ischemic brain damage are cellular and vascular approaches. The vascular strategy aims to rapidly re-open obstructed blood vessels, while the cellular approach aims to interfere with the signalling pathways that facilitate neuron damage and death. Unfortunately, popular vascular treatments have adverse side effects, necessitating the need for alternative chemotherapeutics. In this study, cyclooxygenase-1 (COX-1), which plays a significant role in the post- ischemic neuroinflammation and neuronal death, was targeted for identification of novel drug compounds and to assess the effect of nsSNPs on its structure and function. In a drug discovery part, ligands from the South African Natural Compounds Database (SANCDB-https://sancdb.rubi.ru.ac.za/) and ZINC database (http://zinc15.docking.org/) were used for high-throughput virtual screening (HVTS) against COX-1. Additionally, five nsSNPs were being investigated to assess their impact on protein structure and function. Three of these SNPs were in the COX-1 dimer interface. Molecular docking and molecular dynamics simulations revealed asymmetric nature of the protein. Several ligands, peculiar to each monomer, exhibited favourable binding energies in the respective active sites. SNP analysis indicated effects on inter-monomer interactions and protein stability.
- Full Text:
- Authors: Muronzi, Tendai
- Date: 2020
- Subjects: Cerebrovascular disease , Cerebrovascular disease -- Treatment , Cerebrovascular disease -- Chemotherapy , Cyclooxygenases , High throughput screening (Drug development) , Drug development , Molecular dynamics , South African Natural Compounds Database , ZINC database
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/143404 , vital:38243
- Description: Stroke is the third leading cause of death worldwide, with 87% of cases being ischemic stroke. The two primary therapeutic strategies to reduce post-ischemic brain damage are cellular and vascular approaches. The vascular strategy aims to rapidly re-open obstructed blood vessels, while the cellular approach aims to interfere with the signalling pathways that facilitate neuron damage and death. Unfortunately, popular vascular treatments have adverse side effects, necessitating the need for alternative chemotherapeutics. In this study, cyclooxygenase-1 (COX-1), which plays a significant role in the post- ischemic neuroinflammation and neuronal death, was targeted for identification of novel drug compounds and to assess the effect of nsSNPs on its structure and function. In a drug discovery part, ligands from the South African Natural Compounds Database (SANCDB-https://sancdb.rubi.ru.ac.za/) and ZINC database (http://zinc15.docking.org/) were used for high-throughput virtual screening (HVTS) against COX-1. Additionally, five nsSNPs were being investigated to assess their impact on protein structure and function. Three of these SNPs were in the COX-1 dimer interface. Molecular docking and molecular dynamics simulations revealed asymmetric nature of the protein. Several ligands, peculiar to each monomer, exhibited favourable binding energies in the respective active sites. SNP analysis indicated effects on inter-monomer interactions and protein stability.
- Full Text:
Decent work and informal employment: the case of Bulawayo Metropolitan Province (Central Business District) Zimbabwe
- Authors: Bob, Shaka Keny
- Date: 2020
- Subjects: Labor -- Zimbabwe , Informal sector (Economics) -- Zimbabwe , Job creation -- Zimbabwe , Poor -- Zimbabwe -- Employment , Labor policy -- Zimbabwe , Football coaches -- South Africa
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/115241 , vital:34104
- Description: Zimbabwe, similar to other developing countries experiences a high level of informal employment. However, most informal jobs are situated in very poor working conditions and are characterised by decent work deficits. Despite the fact that various studies have shown the importance of the informal economy in that it provides livelihood earning opportunities for the majority of people in the Global South, it has remained a largely forgotten sector in policy making in most countries. It is, therefore, important that informal work be taken seriously and efforts must be made to improve working conditions for the urban working poor in the developing world. The purpose of this study is to investigate on the self-reported experiences of informal workers to understand their perspectives surrounding the concept of decent work in the Zimbabwean context. The case study is the Bulawayo metropolitan province, and this study targeted informal workers who trade within the central business district. The study also aimed to measure the decent work deficit scores between two economic sectors (food and clothing traders). This was done by testing the suitability of the Edward Webster Decent Work Deficit Index as a methodology of measuring decent work at a micro level. The analysis is based on a mixed methods study which was carried out through the use of a semi-structured survey. The study revealed that decent work for the sampled informal workers meant work related improvements, insurances and risk management, right of expression and business advancement skills which closely resembles the International Labour Organisation's conceptualisation of decent work. The study also highlighted that childcare assistance and disability insurance are concepts which remained excluded in the current conceptualisation of decent work. The thesis offers a new policy angle which shows that to promote decent work the concept of heterogeneity must be adopted because inequalities persist within the informal economy. The study also suggested that the Edward Webster Decent Work Deficit Index can be used as an appropriate methodology of monitoring the progress towards achieving decent work at the micro level i.e. industry or individual level. This is because since the formation of the decent work concept; the International Labour Organisation has only provided a methodology of how to measure the progress of decent work at the county level. The survey findings revealed that food vendors scored more poorly on the decent work deficit index compared to the clothing traders. The study also identified that food vendors and clothing traders are faced with different challenges which suggests that policy makers must take that into consideration when attempting to design policies or programmes which are aimed at assisting informal workers.
- Full Text:
- Authors: Bob, Shaka Keny
- Date: 2020
- Subjects: Labor -- Zimbabwe , Informal sector (Economics) -- Zimbabwe , Job creation -- Zimbabwe , Poor -- Zimbabwe -- Employment , Labor policy -- Zimbabwe , Football coaches -- South Africa
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/115241 , vital:34104
- Description: Zimbabwe, similar to other developing countries experiences a high level of informal employment. However, most informal jobs are situated in very poor working conditions and are characterised by decent work deficits. Despite the fact that various studies have shown the importance of the informal economy in that it provides livelihood earning opportunities for the majority of people in the Global South, it has remained a largely forgotten sector in policy making in most countries. It is, therefore, important that informal work be taken seriously and efforts must be made to improve working conditions for the urban working poor in the developing world. The purpose of this study is to investigate on the self-reported experiences of informal workers to understand their perspectives surrounding the concept of decent work in the Zimbabwean context. The case study is the Bulawayo metropolitan province, and this study targeted informal workers who trade within the central business district. The study also aimed to measure the decent work deficit scores between two economic sectors (food and clothing traders). This was done by testing the suitability of the Edward Webster Decent Work Deficit Index as a methodology of measuring decent work at a micro level. The analysis is based on a mixed methods study which was carried out through the use of a semi-structured survey. The study revealed that decent work for the sampled informal workers meant work related improvements, insurances and risk management, right of expression and business advancement skills which closely resembles the International Labour Organisation's conceptualisation of decent work. The study also highlighted that childcare assistance and disability insurance are concepts which remained excluded in the current conceptualisation of decent work. The thesis offers a new policy angle which shows that to promote decent work the concept of heterogeneity must be adopted because inequalities persist within the informal economy. The study also suggested that the Edward Webster Decent Work Deficit Index can be used as an appropriate methodology of monitoring the progress towards achieving decent work at the micro level i.e. industry or individual level. This is because since the formation of the decent work concept; the International Labour Organisation has only provided a methodology of how to measure the progress of decent work at the county level. The survey findings revealed that food vendors scored more poorly on the decent work deficit index compared to the clothing traders. The study also identified that food vendors and clothing traders are faced with different challenges which suggests that policy makers must take that into consideration when attempting to design policies or programmes which are aimed at assisting informal workers.
- Full Text:
Default in payment, an application of statistical learning techniques
- Authors: Gcakasi, Lulama
- Date: 2020
- Subjects: Credit -- South Africa -- Risk assessment , Risk management -- Statistical methods -- South Africa , Credit -- Management -- Statistical methods , Commercial statistics
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/141547 , vital:37984
- Description: The ability of financial institutions to detect whether a customer will default on their credit card payment is essential for its profitability. To that effect, financial institutions have credit scoring systems in place to be able to estimate the credit risk associated with a customer. Various classification models are used to develop credit scoring systems such as k-nearest neighbours, logistic regression and classification trees. This study aims to assess the performance of different classification models on the prediction of credit card payment default. Credit data is usually of high dimension and as a result dimension reduction techniques, namely principal component analysis and linear discriminant analysis, are used in this study as a means to improve model performance. Two classification models are used, namely neural networks and support vector machines. Model performance is evaluated using accuracy and area under the curve (AUC). The neuarl network classifier performed better than the support vector machine classifier as it produced higher accuracy rates and AUC values. Dimension reduction techniques were not effective in improving model performance but did result in less computationally expensive models.
- Full Text:
- Authors: Gcakasi, Lulama
- Date: 2020
- Subjects: Credit -- South Africa -- Risk assessment , Risk management -- Statistical methods -- South Africa , Credit -- Management -- Statistical methods , Commercial statistics
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/141547 , vital:37984
- Description: The ability of financial institutions to detect whether a customer will default on their credit card payment is essential for its profitability. To that effect, financial institutions have credit scoring systems in place to be able to estimate the credit risk associated with a customer. Various classification models are used to develop credit scoring systems such as k-nearest neighbours, logistic regression and classification trees. This study aims to assess the performance of different classification models on the prediction of credit card payment default. Credit data is usually of high dimension and as a result dimension reduction techniques, namely principal component analysis and linear discriminant analysis, are used in this study as a means to improve model performance. Two classification models are used, namely neural networks and support vector machines. Model performance is evaluated using accuracy and area under the curve (AUC). The neuarl network classifier performed better than the support vector machine classifier as it produced higher accuracy rates and AUC values. Dimension reduction techniques were not effective in improving model performance but did result in less computationally expensive models.
- Full Text:
Design ethnography: methodological considerations for rebalancing socio-technical phenomena in CMS performativity
- Authors: Jordaan, Leandra
- Date: 2020
- Subjects: Database management -- Social aspects , Information technology -- Social aspects , Management information systems -- Social aspects , Database management -- Philosophy , Information technology -- Philosophy , Management information systems -- Philosophy
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164781 , vital:41164
- Description: As a practitioner, I had been part of two Content Management System (CMS) Projects in a particular setting that did not deliver on the expected outcomes. The expected outcomes of the projects included streamlined processes, better access to information, functionalities to drive engagement, reduced time searching for information, and improved reusability of content. As I began to reflect on the projects, a certain discomfort developed when the social aspect was ignored in favour of the technological. I sought a better understanding, but I was unaware of the fact at first. The techno-centricity as a concept found in literature and my experience as content management (CM) practitioner – together informed my interest in rebalancing the social and the technological. Thus, I focused on conduct in performing of tasks and emphasised attention on the practices of experts who gave insight into context of CM and use. The research participants are communication practitioners who have the responsibility to communicate internally and externally on behalf of a publicly-funded university in South Africa. Guided by the abductive argument and literature, the key themes for the research focused on technology, individuals and the organisation. The focus on technology (CMS as a subfield of Information Systems) began with my involvement in two CMS Projects. What came to the fore was that the practitioner perspective, and thus their experiential knowledge are neglected in research and that it may be an over-emphasis on techno-centrism in CMS research. I sought to better balance the social and technological aspects. The second focus on individuals (communication practitioners) was to value the stakeholder who was considered representative of the worldviews of the community. I sought to support corporate communication practices in the task of relationship building with stakeholders. The findings showed that the culture of informatio sharing was low at the University. The third focus on the organisation (the University) was to prioritise the success of the communication undertaken by the University. I interrogated the role of power. I demonstrated how the role of power could be seen as productive and positive in the context of HE, the social reality of CUT, and this IS research itself. I positioned the research as interpretivistic. I used hermeneutics as a mode of analysis because as researcher-practitioner, I am part of the social reality of the research. A safeguard against my subjectivity was the use of Phronesis as an orientation to knowledge. An organisation with a shared understanding of ethics and values will balance instrumental rationality (scientific and technical reasoning) with value-rationality (moral reasoning about right and wrong actions). Phronesis is the virtue that should ensure ethical behaviour in actions, yet over time, social science research, in a quest to mimic the natural sciences, centred on episteme and techne. I constructed the lens by particularising the four value-rational questions of Flyvbjerg’s contemporary Phronesis to the performative perspective of the research. My interest in social reality, as well as socio-technical aspects, have led to design-ethnography (D-E) to emerge as the most suitable and most appropriate approach to the research. D-E asked the prescriptive ‘How’ and descriptive ‘What’ questions, whilst the Flyvbjergian Phronesis lens brought focus on the ‘Why’ question. D-E emphasised the betterment of the interaction and synergy between research conducted and practice. My status as an immersed researcher needed careful consideration, and D-E accepted that a certain level of immersion is necessary, D-E has short interventional fieldwork, and D-E has future-oriented engagement. The contemporary Phronesis used in the research is to enact D-E and not an all-encompassing guide for doing Phronesis research The key contributions to the research are predominantly methodological. The claims draw on evidence focused on the three themes technology, individuals and organisation, and the phenomena of interest which are to rebalance the social and the technological in CM practices. The first claim is that immersion in the context is possible and supported by the specific particularised approaches to the research. The second claim suggests a way to rebalance the socio-technical nature of Information Systems (IS). The third claim suggests that there is a viewpoint for power that is not focused on power’s oppressive nature which could see a diverging focus on power in IS research in future. In this viewpoint, the status quo of social reality is not questioned, nor are their emancipatory ambitions. The fourth and final claim suggests that Phronesis as a lens could enact D-E, which in turn is a method that assists in valuing the experience of the practitioner and the support of collaborative work. The research is recommended for IT/IS practitioners who prefer to value participants’ views in design. The approaches used could be of value for researchers who consider the context of their research to be of higher importance than the generalisation of outcomes. Researchers who find themselves in the position of being immersed in the context but question the correctness of active engagement in the field may discover some value from the research approaches and my experience. Also, researchers or practitioners who have an interest in the balance between social and the technological aspects of IS projects. Researchers or practitioners interested in Phronesis, and perhaps the viewpoint of a lens may also find value in this research.
- Full Text:
- Authors: Jordaan, Leandra
- Date: 2020
- Subjects: Database management -- Social aspects , Information technology -- Social aspects , Management information systems -- Social aspects , Database management -- Philosophy , Information technology -- Philosophy , Management information systems -- Philosophy
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164781 , vital:41164
- Description: As a practitioner, I had been part of two Content Management System (CMS) Projects in a particular setting that did not deliver on the expected outcomes. The expected outcomes of the projects included streamlined processes, better access to information, functionalities to drive engagement, reduced time searching for information, and improved reusability of content. As I began to reflect on the projects, a certain discomfort developed when the social aspect was ignored in favour of the technological. I sought a better understanding, but I was unaware of the fact at first. The techno-centricity as a concept found in literature and my experience as content management (CM) practitioner – together informed my interest in rebalancing the social and the technological. Thus, I focused on conduct in performing of tasks and emphasised attention on the practices of experts who gave insight into context of CM and use. The research participants are communication practitioners who have the responsibility to communicate internally and externally on behalf of a publicly-funded university in South Africa. Guided by the abductive argument and literature, the key themes for the research focused on technology, individuals and the organisation. The focus on technology (CMS as a subfield of Information Systems) began with my involvement in two CMS Projects. What came to the fore was that the practitioner perspective, and thus their experiential knowledge are neglected in research and that it may be an over-emphasis on techno-centrism in CMS research. I sought to better balance the social and technological aspects. The second focus on individuals (communication practitioners) was to value the stakeholder who was considered representative of the worldviews of the community. I sought to support corporate communication practices in the task of relationship building with stakeholders. The findings showed that the culture of informatio sharing was low at the University. The third focus on the organisation (the University) was to prioritise the success of the communication undertaken by the University. I interrogated the role of power. I demonstrated how the role of power could be seen as productive and positive in the context of HE, the social reality of CUT, and this IS research itself. I positioned the research as interpretivistic. I used hermeneutics as a mode of analysis because as researcher-practitioner, I am part of the social reality of the research. A safeguard against my subjectivity was the use of Phronesis as an orientation to knowledge. An organisation with a shared understanding of ethics and values will balance instrumental rationality (scientific and technical reasoning) with value-rationality (moral reasoning about right and wrong actions). Phronesis is the virtue that should ensure ethical behaviour in actions, yet over time, social science research, in a quest to mimic the natural sciences, centred on episteme and techne. I constructed the lens by particularising the four value-rational questions of Flyvbjerg’s contemporary Phronesis to the performative perspective of the research. My interest in social reality, as well as socio-technical aspects, have led to design-ethnography (D-E) to emerge as the most suitable and most appropriate approach to the research. D-E asked the prescriptive ‘How’ and descriptive ‘What’ questions, whilst the Flyvbjergian Phronesis lens brought focus on the ‘Why’ question. D-E emphasised the betterment of the interaction and synergy between research conducted and practice. My status as an immersed researcher needed careful consideration, and D-E accepted that a certain level of immersion is necessary, D-E has short interventional fieldwork, and D-E has future-oriented engagement. The contemporary Phronesis used in the research is to enact D-E and not an all-encompassing guide for doing Phronesis research The key contributions to the research are predominantly methodological. The claims draw on evidence focused on the three themes technology, individuals and organisation, and the phenomena of interest which are to rebalance the social and the technological in CM practices. The first claim is that immersion in the context is possible and supported by the specific particularised approaches to the research. The second claim suggests a way to rebalance the socio-technical nature of Information Systems (IS). The third claim suggests that there is a viewpoint for power that is not focused on power’s oppressive nature which could see a diverging focus on power in IS research in future. In this viewpoint, the status quo of social reality is not questioned, nor are their emancipatory ambitions. The fourth and final claim suggests that Phronesis as a lens could enact D-E, which in turn is a method that assists in valuing the experience of the practitioner and the support of collaborative work. The research is recommended for IT/IS practitioners who prefer to value participants’ views in design. The approaches used could be of value for researchers who consider the context of their research to be of higher importance than the generalisation of outcomes. Researchers who find themselves in the position of being immersed in the context but question the correctness of active engagement in the field may discover some value from the research approaches and my experience. Also, researchers or practitioners who have an interest in the balance between social and the technological aspects of IS projects. Researchers or practitioners interested in Phronesis, and perhaps the viewpoint of a lens may also find value in this research.
- Full Text:
Design, formulation and evalauation of liposomes co-loaded with human serum Albumin and Rifampicin
- Authors: Bapolisi, Alain Murhimalika
- Date: 2020
- Subjects: Liposomes , Serum albumin , Rifampin , Mycobacterium tuberculosis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/161780 , vital:40670
- Description: Tuberculosis (TB) is a devastating infectious disease caused by Mycobacterium tuberculosis and is the leading cause of death from a single infectious agent. The high morbidity and mortality rates of TB are partly due to factors such as the lengthy regimen (of 6–24 months), the development of drug resistance, and the pathogen location within the macrophages. These, with poor physiochemical properties of existing drugs hamper the effectiveness of the treatment despite the existence of potent antibiotics such as Rifampicin (Rif). Hydrophobicity plagues many drugs, including Rif, which are then particularly affected due to inherently poor intracellular availability. Novel drug delivery approaches are therefore needed in order to optimize the cytotoxic potential of said antitubercular drugs. To improve the bioavailability of hydrophobic drugs, numerous delivery strategies have been developed. Amongst these, the coordination of cytotoxic drugs to therapeutic proteins have shown some success for improved efficacy in the management of illnesses including infectious diseases. Of therapeutic proteins, Human Serum Albumin (HSA) is an attractive drug carrier with interestingbenefits such as low immunogenicity, antioxidant properties and improving cellular uptake ofdrugs through HSA-specific binding sites which are expressed on most cells including macrophages, where M. tuberculosis often resides. Hence, combination of Rif to HSA (Rif-HSA)seems a promising approach for improved intracellular delivery of Rif. However, the in vivo stability of colloidal protein-based therapeutics is mostly challenging and an effective vehicle is needed to control the biological fate of such conjugates.Liposomes seem to be appropriate carriers for the Rif-HSA complex due to their reputable applicability for encapsulating diverse materials (i.e., hydrophobic and hydrophilic compounds or small and complex molecules) and preventing chemical and biological degradation of the cargo. Therefore, the main objective of this study was to simultaneously encapsulate Rif and HSA in liposomes, which, to the best of our knowledge, has not been done before. The dual liposomes (Rif-HSA-lip) were made by a modified “Reverse Phase Evaporation” method (REV), following a Design of Experiments (DOE) approach to determine which factors impact the formulation. In addition, liposomes were made from crude soybean lecithin (CSL), rather than expensive and highly purified lipids. The liposomes were fully characterised, and the encapsulation efficiency (î) was monitored using high-performance liquid chromatography (HPLC). The results were correlated with factors such as organic and aqueous phase composition, as well as the in vitro release profile of Rif. Transmission electron microscopy (TEM) results confirmed the formation of spherical dual liposomes nanoparticles of roughly 200 nm. Dynamic light scattering (DLS) and Zeta potential measurements showed a negative charge (<–45 mV) and with satisfactory polydispersity (PDI<0.5). HSA dramatically improved the aqueous solubility of Rif (from1.9 mg/ml in water to around 4.3 mg/ml in HSA 10% solution) mainly due to Rif-HSA hydrophobic interactions. This resulted in a good î of almost 60% for Rif, despite the presence of bulky HSA in the lipid bilayer. These details were confirmed using proton nuclear magnetic resonance (1H NMR) and Fourier-transform infrared spectroscopy (FTIR). Furthermore, energy dispersive X-ray (EDX) and DLS data suggested the presence of HSA poking out on the surface of liposomes, which is encouraging for potential targeted delivery in the future. The in vitro release studies also depicted a substantial improvement in the diffusion of Rif in dual liposomes versus free Rif, from 65% after 12 hours for free Rif to 95% after only 5 hours for Rif- HSA-lip. Finally, stability studies conducted over 30 days at room temperature, showed that the freeze-dried formulations of Rif-HSA-lip exhibited good shelf stability over liposomes with no HSA. This study represents an illustrative example of co-loading of antibiotics and proteins into liposomes, which could encourage further development of novel nanoparticulate tools for the effective management of both drug-susceptible and -resistant infectious diseases such as TB.
- Full Text:
- Authors: Bapolisi, Alain Murhimalika
- Date: 2020
- Subjects: Liposomes , Serum albumin , Rifampin , Mycobacterium tuberculosis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/161780 , vital:40670
- Description: Tuberculosis (TB) is a devastating infectious disease caused by Mycobacterium tuberculosis and is the leading cause of death from a single infectious agent. The high morbidity and mortality rates of TB are partly due to factors such as the lengthy regimen (of 6–24 months), the development of drug resistance, and the pathogen location within the macrophages. These, with poor physiochemical properties of existing drugs hamper the effectiveness of the treatment despite the existence of potent antibiotics such as Rifampicin (Rif). Hydrophobicity plagues many drugs, including Rif, which are then particularly affected due to inherently poor intracellular availability. Novel drug delivery approaches are therefore needed in order to optimize the cytotoxic potential of said antitubercular drugs. To improve the bioavailability of hydrophobic drugs, numerous delivery strategies have been developed. Amongst these, the coordination of cytotoxic drugs to therapeutic proteins have shown some success for improved efficacy in the management of illnesses including infectious diseases. Of therapeutic proteins, Human Serum Albumin (HSA) is an attractive drug carrier with interestingbenefits such as low immunogenicity, antioxidant properties and improving cellular uptake ofdrugs through HSA-specific binding sites which are expressed on most cells including macrophages, where M. tuberculosis often resides. Hence, combination of Rif to HSA (Rif-HSA)seems a promising approach for improved intracellular delivery of Rif. However, the in vivo stability of colloidal protein-based therapeutics is mostly challenging and an effective vehicle is needed to control the biological fate of such conjugates.Liposomes seem to be appropriate carriers for the Rif-HSA complex due to their reputable applicability for encapsulating diverse materials (i.e., hydrophobic and hydrophilic compounds or small and complex molecules) and preventing chemical and biological degradation of the cargo. Therefore, the main objective of this study was to simultaneously encapsulate Rif and HSA in liposomes, which, to the best of our knowledge, has not been done before. The dual liposomes (Rif-HSA-lip) were made by a modified “Reverse Phase Evaporation” method (REV), following a Design of Experiments (DOE) approach to determine which factors impact the formulation. In addition, liposomes were made from crude soybean lecithin (CSL), rather than expensive and highly purified lipids. The liposomes were fully characterised, and the encapsulation efficiency (î) was monitored using high-performance liquid chromatography (HPLC). The results were correlated with factors such as organic and aqueous phase composition, as well as the in vitro release profile of Rif. Transmission electron microscopy (TEM) results confirmed the formation of spherical dual liposomes nanoparticles of roughly 200 nm. Dynamic light scattering (DLS) and Zeta potential measurements showed a negative charge (<–45 mV) and with satisfactory polydispersity (PDI<0.5). HSA dramatically improved the aqueous solubility of Rif (from1.9 mg/ml in water to around 4.3 mg/ml in HSA 10% solution) mainly due to Rif-HSA hydrophobic interactions. This resulted in a good î of almost 60% for Rif, despite the presence of bulky HSA in the lipid bilayer. These details were confirmed using proton nuclear magnetic resonance (1H NMR) and Fourier-transform infrared spectroscopy (FTIR). Furthermore, energy dispersive X-ray (EDX) and DLS data suggested the presence of HSA poking out on the surface of liposomes, which is encouraging for potential targeted delivery in the future. The in vitro release studies also depicted a substantial improvement in the diffusion of Rif in dual liposomes versus free Rif, from 65% after 12 hours for free Rif to 95% after only 5 hours for Rif- HSA-lip. Finally, stability studies conducted over 30 days at room temperature, showed that the freeze-dried formulations of Rif-HSA-lip exhibited good shelf stability over liposomes with no HSA. This study represents an illustrative example of co-loading of antibiotics and proteins into liposomes, which could encourage further development of novel nanoparticulate tools for the effective management of both drug-susceptible and -resistant infectious diseases such as TB.
- Full Text:
Design, formulation and evaluation of liposomes co-loaded with human serum albumin and rifampicin
- Authors: Bapolisi, Alain Murhimalika
- Date: 2020
- Subjects: Liposomes , Rifampin , Antitubercular agents , Serum albumin , Albumins , Tuberculosis -- Treatment
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/163179 , vital:41016
- Description: Tuberculosis (TB) is a devastating infectious disease caused by Mycobacterium tuberculosis and is the leading cause of death from a single infectious agent. The high morbidity and mortality rates of TB are partly due to factors such as the lengthy regimen (of 6–24 months), the development of drug resistance, and the pathogen location within the macrophages. These, with poor physiochemical properties of existing drugs hamper the effectiveness of the treatment despite the existence of potent antibiotics such as Rifampicin (Rif). Hydrophobicity plagues many drugs, including Rif, which are then particularly affected due to inherently poor intracellular availability. Novel drug delivery approaches are therefore needed in order to optimize the cytotoxic potential of said antitubercular drugs. To improve the bioavailability of hydrophobic drugs, numerous delivery strategies have been developed. Amongst these, the coordination of cytotoxic drugs to therapeutic proteins have shown some success for improved efficacy in the management of illnesses including infectious diseases. Of therapeutic proteins, Human Serum Albumin (HSA) is an attractive drug carrier with interesting benefits such as low immunogenicity, antioxidant properties and improving cellular uptake of drugs through HSA-specific binding sites which are expressed on most cells including macrophages, where M. tuberculosis often resides. Hence, combination of Rif to HSA (Rif-HSA) seems a promising approach for improved intracellular delivery of Rif. However, the in vivo stability of colloidal protein-based therapeutics is mostly challenging and an effective vehicle is needed to control the biological fate of such conjugates. Liposomes seem to be appropriate carriers for the Rif-HSA complex due to their reputable applicability for encapsulating diverse materials (i.e., hydrophobic and hydrophilic compounds or small and complex molecules) and preventing chemical and biological degradation of the cargo. Therefore, the main objective of this study was to simultaneously encapsulate Rif and HSA in liposomes, which, to the best of our knowledge, has not been done before. The dual liposomes (Rif-HSA-lip) were made by a modified “Reverse Phase Evaporation” method (REV), following a Design of Experiments (DOE) approach to determine which factors impact the formulation. In addition, liposomes were made from crude soybean lecithin (CSL), rather than expensive and highly purified lipids. iv The liposomes were fully characterised, and the encapsulation efficiency (î) was monitored using high-performance liquid chromatography (HPLC). The results were correlated with factors such as organic and aqueous phase composition, as well as the in vitro release profile of Rif. Transmission electron microscopy (TEM) results confirmed the formation of spherical dual liposomes nanoparticles of roughly 200 nm. Dynamic light scattering (DLS) and Zeta potential measurements showed a negative charge (<–45 mV) and with satisfactory polydispersity (PDI<0.5). HSA dramatically improved the aqueous solubility of Rif (from1.9 mg/ml in water to around 4.3 mg/ml in HSA 10% solution) mainly due to Rif-HSA hydrophobic interactions. This resulted in a good î of almost 60% for Rif, despite the presence of bulky HSA in the lipid bilayer. These details were confirmed using proton nuclear magnetic resonance (1H NMR) and Fourier-transform infrared spectroscopy (FTIR). Furthermore, energy dispersive X-ray (EDX) and DLS data suggested the presence of HSA poking out on the surface of liposomes, which is encouraging for potential targeted delivery in the future. The in vitro release studies also depicted a substantial improvement in the diffusion of Rif in dual liposomes versus free Rif, from 65% after 12 hours for free Rif to 95% after only 5 hours for Rif- HSA-lip. Finally, stability studies conducted over 30 days at room temperature, showed that the freeze-dried formulations of Rif-HSA-lip exhibited good shelf stability over liposomes with no HSA. This study represents an illustrative example of co-loading of antibiotics and proteins into liposomes, which could encourage further development of novel nanoparticulate tools for the effective management of both drug-susceptible and -resistant infectious diseases such as TB.
- Full Text:
- Authors: Bapolisi, Alain Murhimalika
- Date: 2020
- Subjects: Liposomes , Rifampin , Antitubercular agents , Serum albumin , Albumins , Tuberculosis -- Treatment
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/163179 , vital:41016
- Description: Tuberculosis (TB) is a devastating infectious disease caused by Mycobacterium tuberculosis and is the leading cause of death from a single infectious agent. The high morbidity and mortality rates of TB are partly due to factors such as the lengthy regimen (of 6–24 months), the development of drug resistance, and the pathogen location within the macrophages. These, with poor physiochemical properties of existing drugs hamper the effectiveness of the treatment despite the existence of potent antibiotics such as Rifampicin (Rif). Hydrophobicity plagues many drugs, including Rif, which are then particularly affected due to inherently poor intracellular availability. Novel drug delivery approaches are therefore needed in order to optimize the cytotoxic potential of said antitubercular drugs. To improve the bioavailability of hydrophobic drugs, numerous delivery strategies have been developed. Amongst these, the coordination of cytotoxic drugs to therapeutic proteins have shown some success for improved efficacy in the management of illnesses including infectious diseases. Of therapeutic proteins, Human Serum Albumin (HSA) is an attractive drug carrier with interesting benefits such as low immunogenicity, antioxidant properties and improving cellular uptake of drugs through HSA-specific binding sites which are expressed on most cells including macrophages, where M. tuberculosis often resides. Hence, combination of Rif to HSA (Rif-HSA) seems a promising approach for improved intracellular delivery of Rif. However, the in vivo stability of colloidal protein-based therapeutics is mostly challenging and an effective vehicle is needed to control the biological fate of such conjugates. Liposomes seem to be appropriate carriers for the Rif-HSA complex due to their reputable applicability for encapsulating diverse materials (i.e., hydrophobic and hydrophilic compounds or small and complex molecules) and preventing chemical and biological degradation of the cargo. Therefore, the main objective of this study was to simultaneously encapsulate Rif and HSA in liposomes, which, to the best of our knowledge, has not been done before. The dual liposomes (Rif-HSA-lip) were made by a modified “Reverse Phase Evaporation” method (REV), following a Design of Experiments (DOE) approach to determine which factors impact the formulation. In addition, liposomes were made from crude soybean lecithin (CSL), rather than expensive and highly purified lipids. iv The liposomes were fully characterised, and the encapsulation efficiency (î) was monitored using high-performance liquid chromatography (HPLC). The results were correlated with factors such as organic and aqueous phase composition, as well as the in vitro release profile of Rif. Transmission electron microscopy (TEM) results confirmed the formation of spherical dual liposomes nanoparticles of roughly 200 nm. Dynamic light scattering (DLS) and Zeta potential measurements showed a negative charge (<–45 mV) and with satisfactory polydispersity (PDI<0.5). HSA dramatically improved the aqueous solubility of Rif (from1.9 mg/ml in water to around 4.3 mg/ml in HSA 10% solution) mainly due to Rif-HSA hydrophobic interactions. This resulted in a good î of almost 60% for Rif, despite the presence of bulky HSA in the lipid bilayer. These details were confirmed using proton nuclear magnetic resonance (1H NMR) and Fourier-transform infrared spectroscopy (FTIR). Furthermore, energy dispersive X-ray (EDX) and DLS data suggested the presence of HSA poking out on the surface of liposomes, which is encouraging for potential targeted delivery in the future. The in vitro release studies also depicted a substantial improvement in the diffusion of Rif in dual liposomes versus free Rif, from 65% after 12 hours for free Rif to 95% after only 5 hours for Rif- HSA-lip. Finally, stability studies conducted over 30 days at room temperature, showed that the freeze-dried formulations of Rif-HSA-lip exhibited good shelf stability over liposomes with no HSA. This study represents an illustrative example of co-loading of antibiotics and proteins into liposomes, which could encourage further development of novel nanoparticulate tools for the effective management of both drug-susceptible and -resistant infectious diseases such as TB.
- Full Text:
Detecting the transnational space: metaphysical detecting in Kazuo Ishiguro’s When we were orphans, Michiel Heyns’s Lost ground, and Amitav Gosh’s The Calcutta chromosome
- Authors: Botes, Niki
- Date: 2020
- Subjects: Literature and transnationalism , Detective and mystery stories -- History and criticism , Ishiguro, Kazuo, 1954- , Ishiguro, Kazuo, 1954- -- When we were orpahns , Heyns, Michiel , Heyns, Michiel -- Lost ground , Ghosh, Amitav, 1956- , Ghosh, Amitav, 1956- -- The Calcutta chromosome
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/140971 , vital:37933
- Description: The transnational detectives in the three primary texts under scrutiny—Kazuo Ishiguro’s When We Were Orphans, Michiel Heyns’s Lost Ground, and Amitav Ghosh’s The Calcutta Chromosome—are regarded as metaphysical detectives in this thesis. As transnational figures, they criss-cross physical and national borders, but their investigations also often require the crossing of epistemological and ontological borders, as well as thinking outside of binaries. The fictional transnational, postcolonial worlds presented in these texts all feature a lack of change and the persistence of the socio-economic inequalities and discriminations of the colonial or, in the case of Lost Ground, the apartheid past. This thesis will argue that the three texts foreground the inability to break free from conceptual frameworks and a lack in human relationships as two possible reasons why the communities and societies in question have not been able to affect change. To solve the various mysteries in the texts, the transnational detectives need to find new ways of knowing and being. The lessons that these detectives learn and the personal changes they experience serve as synecdoche for how a better tomorrow for the transnational, postcolonial world could possibly be achieved. The methodology employed in this thesis involves a comparative reading of the three primary texts. To analyse and interpret these texts, this thesis draws on the philosophies of postmodern philosophers such as Martin Heidegger, Michel Foucault, Jacques Derrida, and Emmanuel Levinas, as well as postcolonial theorists like Edward Said, Gayatri Chakravorty Spivak, and Homi K. Bhabha. The thesis will contend that especially Derrida’s work on deconstruction, which forms the conceptual basis for Bhabha’s notion of the ‘third space,’ as well as Levinas’s anti-totalitarian approach with regard to the ethical relationship between the self and the other, are viable better ways of knowing and being in the transnational, postcolonial worlds presented in the texts. Derrida’s notion of différance and the trace, as well as Bhabha’s third space, this thesis contends, completely destabilises binary thinking and offers ways to think outside of the limited frameworks of ideological discourse. This thesis also posits that Levinas’s metaphysical and pluralistic interpretation of how the self should, and can, relate to the other, presents a better way of forming human relationships and has the potential to affect positive change.
- Full Text:
- Authors: Botes, Niki
- Date: 2020
- Subjects: Literature and transnationalism , Detective and mystery stories -- History and criticism , Ishiguro, Kazuo, 1954- , Ishiguro, Kazuo, 1954- -- When we were orpahns , Heyns, Michiel , Heyns, Michiel -- Lost ground , Ghosh, Amitav, 1956- , Ghosh, Amitav, 1956- -- The Calcutta chromosome
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/140971 , vital:37933
- Description: The transnational detectives in the three primary texts under scrutiny—Kazuo Ishiguro’s When We Were Orphans, Michiel Heyns’s Lost Ground, and Amitav Ghosh’s The Calcutta Chromosome—are regarded as metaphysical detectives in this thesis. As transnational figures, they criss-cross physical and national borders, but their investigations also often require the crossing of epistemological and ontological borders, as well as thinking outside of binaries. The fictional transnational, postcolonial worlds presented in these texts all feature a lack of change and the persistence of the socio-economic inequalities and discriminations of the colonial or, in the case of Lost Ground, the apartheid past. This thesis will argue that the three texts foreground the inability to break free from conceptual frameworks and a lack in human relationships as two possible reasons why the communities and societies in question have not been able to affect change. To solve the various mysteries in the texts, the transnational detectives need to find new ways of knowing and being. The lessons that these detectives learn and the personal changes they experience serve as synecdoche for how a better tomorrow for the transnational, postcolonial world could possibly be achieved. The methodology employed in this thesis involves a comparative reading of the three primary texts. To analyse and interpret these texts, this thesis draws on the philosophies of postmodern philosophers such as Martin Heidegger, Michel Foucault, Jacques Derrida, and Emmanuel Levinas, as well as postcolonial theorists like Edward Said, Gayatri Chakravorty Spivak, and Homi K. Bhabha. The thesis will contend that especially Derrida’s work on deconstruction, which forms the conceptual basis for Bhabha’s notion of the ‘third space,’ as well as Levinas’s anti-totalitarian approach with regard to the ethical relationship between the self and the other, are viable better ways of knowing and being in the transnational, postcolonial worlds presented in the texts. Derrida’s notion of différance and the trace, as well as Bhabha’s third space, this thesis contends, completely destabilises binary thinking and offers ways to think outside of the limited frameworks of ideological discourse. This thesis also posits that Levinas’s metaphysical and pluralistic interpretation of how the self should, and can, relate to the other, presents a better way of forming human relationships and has the potential to affect positive change.
- Full Text:
Determination of nonlinear optical properties of phthalocyanine regioisomers using computational models
- Date: 2020
- Subjects: Electrochemistry , Phthalocyanines , Nanoparticles , Nonlinear optics , Nonlinear optical spectroscopy , Refraction
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/166197 , vital:41337
- Description: This work investigates the effects of the nonlinear optical properties of four different constitutional isomers (C4h, C2v, Cs, and D2h) of a series of tetrasubstituted phthalocyanines (free-base 3-4-tert-butylphenoxyether phthalocyanines, free-base 4-4-tertbutylphenoxyether phthalocyanines, SnCl2 tetra substituted 3-4-tert-butylphenoxyether phthalocyanine, and SnCl2 tetra substituted 4-4-tert-butylphenoxyether phthalocyanine). The properties investigated were the real and imaginary components of the 3rd order hyperpolarizability, as well as the excited state absorption and refraction cross sections. The investigations were performed with a z-scan over a range of laser beam intensities. This work determined the imaginary component of the 3rd order hyperpolarizability for the free-base and SnCl2 3-4-tert-butylphenoxyether phthalocyanines and 4-4-tert-butylphenoxyether phthalocyanines to be highly dependent on the excited state cross sections. The refraction caused due to the real component of the 3rd order hyperpolarizability of the phthalocyanines was also investigated, however, the values found were strongly dependent on the laser beam intensity and the cause of this was investigated. A Five-level model was developed and run on GPGPU computing devices in order to isolate the absorption and refractive cross sections. Theeffects of the regio substitution on the excited state cross sections were also investigated, and the 1st singlet excited state and 1st triplet state absorption cross sections were calculated for all constitutional isomers. It was found that the symmetry of the constitutional isomers have a disproportionately large effect on the excited state absorption when compared to the ground state absorption. The nonlinear refractive properties of all constitutional isomers were also investigated, and the values of the parametric susceptibility are reported herein. The nonlinear refraction was found to have less effect than was seen in the nonlinear absorption. The 1st singlet excited state and 1st triplet state refractive cross sections of all constitutional isomer was determined. The results indicated that if more than one excited state was present and contributing to the nonlinear refraction, then more data than was collected here would be required. However, the 1st singlet excited state cross section were successfully determined for the free-base constitutional isomers. This work concluded that the region substitution affected the excited states more than the ground state.
- Full Text:
- Date: 2020
- Subjects: Electrochemistry , Phthalocyanines , Nanoparticles , Nonlinear optics , Nonlinear optical spectroscopy , Refraction
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/166197 , vital:41337
- Description: This work investigates the effects of the nonlinear optical properties of four different constitutional isomers (C4h, C2v, Cs, and D2h) of a series of tetrasubstituted phthalocyanines (free-base 3-4-tert-butylphenoxyether phthalocyanines, free-base 4-4-tertbutylphenoxyether phthalocyanines, SnCl2 tetra substituted 3-4-tert-butylphenoxyether phthalocyanine, and SnCl2 tetra substituted 4-4-tert-butylphenoxyether phthalocyanine). The properties investigated were the real and imaginary components of the 3rd order hyperpolarizability, as well as the excited state absorption and refraction cross sections. The investigations were performed with a z-scan over a range of laser beam intensities. This work determined the imaginary component of the 3rd order hyperpolarizability for the free-base and SnCl2 3-4-tert-butylphenoxyether phthalocyanines and 4-4-tert-butylphenoxyether phthalocyanines to be highly dependent on the excited state cross sections. The refraction caused due to the real component of the 3rd order hyperpolarizability of the phthalocyanines was also investigated, however, the values found were strongly dependent on the laser beam intensity and the cause of this was investigated. A Five-level model was developed and run on GPGPU computing devices in order to isolate the absorption and refractive cross sections. Theeffects of the regio substitution on the excited state cross sections were also investigated, and the 1st singlet excited state and 1st triplet state absorption cross sections were calculated for all constitutional isomers. It was found that the symmetry of the constitutional isomers have a disproportionately large effect on the excited state absorption when compared to the ground state absorption. The nonlinear refractive properties of all constitutional isomers were also investigated, and the values of the parametric susceptibility are reported herein. The nonlinear refraction was found to have less effect than was seen in the nonlinear absorption. The 1st singlet excited state and 1st triplet state refractive cross sections of all constitutional isomer was determined. The results indicated that if more than one excited state was present and contributing to the nonlinear refraction, then more data than was collected here would be required. However, the 1st singlet excited state cross section were successfully determined for the free-base constitutional isomers. This work concluded that the region substitution affected the excited states more than the ground state.
- Full Text:
Developing an organisational culture in support of organisational capacity for change: the case of a South African bank
- Authors: Dhoba, Howard
- Date: 2020
- Subjects: Corporate culture , Bank management , Organizational change
- Language: English
- Type: text , Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10962/141247 , vital:37956
- Description: Business organisations are increasingly being exposed to challenging environmental pressures. It has been widely acknowledged and observed that an organisation that crafts an effective organisational culture should be well positioned to react to these threatening changes, and to proactively seize opportunities to adapt and learn. This research study seeks to investigate how an organisational culture that supports organisational capacity for change can be developed. It analyses what a banking organisation’s espoused culture is made up of and how it was formulated. A thematic analysis of how the bank’s aspirational and innovative culture contributes to the organisation’s capacity for change is also presented. Organisational management should take a leading role in developing the values and practices that foster participation, which are then imparted to the entire organisation. The research study thus only focused on analysing the development of organisational culture from the management perspective. An interpretivist research approach was adopted, with a case study design as the research method. Data was gathered through conducting personal interviews with twelve managers from a Retail and Business Banking cluster of one of the big South African banks. Numerous interventions were employed in the organisation to develop espoused values, aspirational culture and to encourage all employees to champion innovations and constantly search for better ways to offer delighting value propositions to clients. The research findings can assist managers and organisational leaders in building their organisational capacity for change through developing an effective organisational culture that supports change initiatives. Since the research study only focused on analysing the development of organisational culture from the perspective of management, further research studies may involve employees as well, due to the fact that everyone is expected to take an active role for an organisation to display the overall capability to effectively respond to a progressively volatile environment. The research was able to reveal how key initiatives that are designed from theoretical constructs can be used to deliver on an innovative organisational culture that contributes to organisational capability for change.
- Full Text:
- Authors: Dhoba, Howard
- Date: 2020
- Subjects: Corporate culture , Bank management , Organizational change
- Language: English
- Type: text , Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10962/141247 , vital:37956
- Description: Business organisations are increasingly being exposed to challenging environmental pressures. It has been widely acknowledged and observed that an organisation that crafts an effective organisational culture should be well positioned to react to these threatening changes, and to proactively seize opportunities to adapt and learn. This research study seeks to investigate how an organisational culture that supports organisational capacity for change can be developed. It analyses what a banking organisation’s espoused culture is made up of and how it was formulated. A thematic analysis of how the bank’s aspirational and innovative culture contributes to the organisation’s capacity for change is also presented. Organisational management should take a leading role in developing the values and practices that foster participation, which are then imparted to the entire organisation. The research study thus only focused on analysing the development of organisational culture from the management perspective. An interpretivist research approach was adopted, with a case study design as the research method. Data was gathered through conducting personal interviews with twelve managers from a Retail and Business Banking cluster of one of the big South African banks. Numerous interventions were employed in the organisation to develop espoused values, aspirational culture and to encourage all employees to champion innovations and constantly search for better ways to offer delighting value propositions to clients. The research findings can assist managers and organisational leaders in building their organisational capacity for change through developing an effective organisational culture that supports change initiatives. Since the research study only focused on analysing the development of organisational culture from the perspective of management, further research studies may involve employees as well, due to the fact that everyone is expected to take an active role for an organisation to display the overall capability to effectively respond to a progressively volatile environment. The research was able to reveal how key initiatives that are designed from theoretical constructs can be used to deliver on an innovative organisational culture that contributes to organisational capability for change.
- Full Text: