Cleaning of fouled membranes using enzymes from a sulphidogenic bioreactor
- Authors: Melamane, Xolisa
- Date: 2004
- Subjects: Membrane filters , Membrane filters -- Fouling , Enzymes -- Biotechnology , Enzymes -- Purification , Water -- Purification -- Membrane filtration , Ultrafiltration
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4133 , http://hdl.handle.net/10962/d1015764
- Description: Maintenance of membrane performance requires inevitable cleaning or defouling of fouled membranes. Membrane cleaning using enzymes such as proteases, lipases, α-glucosidases from a sulphidogenic bioreactor was investigated. At first, dilute and concentrated enzyme extract were prepared form the sulphidogenic pellet. Enzyme assays on 0.5 % azocaisen, 1 % triacetin and 1 mg/ml ρ-nitrophenyl-α-D-glucopyranoside were performed using the concentrated enzyme extract (0 – 200 mg/ml). For membrane fouling, an abattoir effluent was obtained from Ostritech Pty (Ltd), Grahamstown, South Africa. The effluent was characterised for presence of potential foulants such as lipids, proteins, amino acids and carbohydrates. Static fouling of polysulphone membranes (0.22 μm, 47 mm) was then performed using the abattoir effluent. Cleaning of the fouled membranes was also performed using at first the dilute and then the concentrated form (200 mg/ml) of enzyme extracts. Qualitative and quantitative biochemical analysis for proteins, lipids and carbohydrates was performed to ascertain the presence of foulants on polysulphone membranes and their removal by dilute or concentrated enzyme extracts. The ability of dilute enzyme extracts to remove proteins lipids, and carbohydrates fouling capillary UF membrane module; their ability to restore permeate fluxes and transmembrane pressure after cleaning/defouling was also investigated. Permeate volumes from this UF membrane module were analysed for protein, amino acids, lipids, and carbohydrates concentrations after fouling and defouling. Fouling was further characterized by standard blocking, cake filtration and pore blocking models using stirred UF cell and polyethersulphone membranes with MWCO of 30 000, 100 000 and 300 000. After characterization of fouling, polyethersulphone membranes with MWCO of 30 000 and 300 000 were defouled using the concentrated enzyme extract (100 mg ml). Enzyme activities at 200 mg/ml of enzyme concentration were 8.071 IU, 86.71 IU and 789.02 IU for proteases, lipases and α-glucosidases. The abattoir effluent contained 553 μg/ml of lipid, 301 μg/ml of protein, 141 μg/ml of total carbohydrate, and 0.63 μg/ml of total reducing sugars. Proteins, lipids and carbohydrates fouling polysulphone membranes after a day were removed by 23.4 %, when a dilute enzyme was used. A concentrated enzyme extract of 200 mg/ml was able to remove proteins, lipids and carbohydrates up to 5 days of fouling by 100 %, 82 %, 71 %, 68 % and 76 % respectively. Defouling of dynamically fouled capillary ultrafiltration membranes using sulphidogenic proteases was successful at pH 10, 37°C, within 1 hour. Sulphidogenic proteases activity was 2.1 U/ml and flux Recovery (FR %) was 64. Characterization of fouling revealed that proteins and lipids were major foulants while low concentration of carbohydrates fouled polyethersulphone membranes. Fouling followed standard blocking for 10 minutes in all the membranes; afterwards fouling adopted cake filtration model for membranes with 30 000 MWCO and pore blocking model for membranes with 300 000 MWCO. A concentration of 100 mg/ml of enzyme extract was able to remove fouling from membranes with MWCO of 30 000. Defouling membranes that followed pore blocking model i.e. 300 000 MWCO was not successful due to a mass transfer problem. From the results of defouling of 30 000 and 300 000 MWCO it was concluded that defouling of cake layer fouling (30 000 MWCO) was successful while defouling of pore blocking fouling was unsuccessful due to a mass transfer problem. The ratio of enzymes present in the enzyme extract when calculated based on enzymatic activity for proteases, lipases and α-glucosidases was 1.1 %, 11 % and 87.9 %. It was hypothesized that apart from proteases, lipases, α and β-glucosidases; phosphatases, sulphatases, amonipeptidases etc. from a sulphidogenic bioreactor clean or defoul cake layer fouling by organic foulants and pore blocking fouling provided the mass transfer problem is solved. However, concentration of enzymes from a sulphidogenic bioreactor has not been optimized yet. Other methods of concentrating the enzyme extract can be investigated for example use of organic solvents.
- Full Text:
Competition for attachment of aquaculture candidate probiotic and pathogenic bacteria on fish intestinal mucus:
- Authors: Vine, Niall G , Leukes, Winston D , Kaiser, Horst , Daya, Santylal , Baxter, Jeremy , Hecht, Thomas
- Date: 2004
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142819 , vital:38120 , DOI: 10.1111/j.1365-2761.2004.00542.x
- Description: Probiotics for aquaculture are generally only selected by their ability to produce antimicrobial metabolites; however, attachment to intestinal mucus is important in order to remain within the gut of its host. Five candidate probiotics (AP1–AP5), isolated from the clownfish, Amphiprion percula (Lacepéde), were examined for their ability to attach to fish intestinal mucus and compete with two pathogens, Aeromonas hydrophila and Vibrio alginolyticus. Two different radioactive isotopes were used to quantify competition between pathogens and probionts. Attachment of the pathogens was enhanced by the presence of the candidate probiotics. However, the addition of the candidate probiotics after the pathogens resulted in reduced pathogen attachment.
- Full Text:
Composition portfolio
- Authors: Prince, Lloyd T
- Date: 2004
- Subjects: Music -- South Africa Composition (Music)
- Language: English
- Type: Thesis , Masters , MMus
- Identifier: vital:2652 , http://hdl.handle.net/10962/d1002318
- Full Text:
Concerning secondary thermoluminescence peaks in α-Al_2_O_3_:C
- Authors: Chithambo, Makaiko L
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6793 , http://hdl.handle.net/10962/d1003823
- Description: Thermoluminescence characteristics of two subsidiary glow peaks, one below 100°C and the other above 300°C, have been studied for measurements between 30°C and 500°C in α-Al_2_O_3_:C. The thermoluminescence intensity of the lower temperature peak decreased with storage with a half-life of about 150 s. In contrast, the intensity of the higher temperature peak increased with storage towards some maximum. The peak-temperature of each of the secondary glow peaks was essentially constant with dose, whereas that of the main peak decreased with irradiation. The dose response for the three peaks was similar except for sublinear growth in the higher temperature peak at low dose values. These effects are discussed in terms of changes in the concentration of F+ luminescence precursors brought about by competitive electron retrapping at deep electron-traps or hole-traps. This work refines the physics and application of α-Al_2_O_3_:C luminescence in radiation dosimetry.
- Full Text:
Constructing a cardinal measure of democratic development in a transition polity: the Nigerian example
- Authors: Dinneya, Godson E , Tsegaye, Asrat
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6076 , http://hdl.handle.net/10962/d1003837
- Description: Existing measures of the level of democracy present in a given state treat democracy as a product and therefore place undue emphasis on actual freedoms enjoyed by the citizens of the country. In transition polities where the actual levels of freedom are low despite continuing efforts to democratize, democracy should be seen as a process rather than a product. A measure that dilutes the end product to capture today's struggles against undemocratic structures and policies does so in order to recognize the foundations these inputs lay for future democratic development. Nigeria exemplifies the many polities in transition on the African continent. This essay looks at the major political events that typify the processes of power change, quality of governance, political environment and democratic dividends, and uses them to construct democratization indices to determine the pattern and level of democratization in Nigeria since political independence. This exercise sets the stage for assessing the impacts of various dimensions of democratization on the performance of the Nigerian economy.
- Full Text:
Contested rights : the impact of game farming on farm workers in the Bushmen's River area
- Authors: Luck, Kelly
- Date: 2004
- Subjects: Game farms -- South Africa -- Eastern Cape , Agricultural laborers -- South Africa -- Eastern Cape -- Social conditions Agricultural laborers -- South Africa -- Eastern Cape -- Economic conditions Agricultural laborers -- Political aspects -- South Africa -- Eastern Cape Agricultural laborers -- Legal status, laws, etc. -- South Africa -- Eastern Cape Agricultural laborers -- Protection -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2104 , http://hdl.handle.net/10962/d1004144
- Description: This thesis is an investigation of the impact of commercial game farming on former farm workers in the Bushman's River area of the Eastern Cape. In its examination of the broader economic and political changes that have facilitated a move from agriculture to game farming, it analyses how these changes affect farm workers. The main concern of the thesis is the ways in which farm workers (at the local level) respond to changes at the national and global level (legal and political changes, the advent of tourism, and the injection of foreign capital and businessmen into the area). Lack of knowledge about their rights under the current political dispensation, as well as the perceived need for mediation between themselves and foreign landowners, points to a general sense of powerlessness. Feelings of alienation from local government structures aimed at fulfilling this function indicate a significant gap between the statute at the national level and the local reality. Local reality is informed by a strong conservatism which is generated by African Independent Church structures and local Xhosa perceptions of manhood and respectability. This conservative discourse leads to a frame of reference which is largely informed by pre-1994 interactions with farmers and government. This results in a situation in which farm workers, largely unaware of their rights in the new dispensation, operate as they did in the past; waiting for landowners to decide their fate for them. What ensues is a lack of meaningful interaction with government and landowners, perpetuating their subjugation and cynicism as to whether government structures are in fact working in their interests. The thesis comes to three main conclusions. The first is that game farming has been negatively received by farm workers due to the associated threats of unemployment and eviction. The second is that despite high levels of subjugation, even the very poor are agents to some degree. The creation of a masculine identity which is internally articulated, as opposed to outwardly expressed, and the grounding of reputation in the family suggest that farm workers have developed mechanisms to deal with their disempowered position. Lastly, farm workers are in possession of social capital which has made it possible for them to deal with their low status in the societal hierarchy. This includes the Church, family and fellow community members. These coping strategies have however proved a disadvantage in the current era because they prevent direct communication with landowners, government and NGOs.
- Full Text:
CREWS : a Component-driven, Run-time Extensible Web Service framework
- Authors: Parry, Dominic Charles
- Date: 2004
- Subjects: Component software -- Development , Computer software -- Reusability , Software reengineering , Web services
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4628 , http://hdl.handle.net/10962/d1006501 , Component software -- Development , Computer software -- Reusability , Software reengineering , Web services
- Description: There has been an increased focus in recent years on the development of re-usable software, in the form of objects and software components. This increase, together with pressures from enterprises conducting transactions on the Web to support all business interactions on all scales, has encouraged research towards the development of easily reconfigurable and highly adaptable Web services. This work investigates the ability of Component-Based Software Development (CBSD) to produce such systems, and proposes a more manageable use of CBSD methodologies. Component-Driven Software Development (CDSD) is introduced to enable better component manageability. Current Web service technologies are also examined to determine their ability to support extensible Web services, and a dynamic Web service architecture is proposed. The work also describes the development of two proof-of-concept systems, DREW Chat and Hamilton Bank. DREW Chat and Hamilton Bank are implementations of Web services that support extension dynamically and at run-time. DREW Chat is implemented on the client side, where the user is given the ability to change the client as required. Hamilton Bank is a server-side implementation, which is run-time customisable by both the user and the party offering the service. In each case, a generic architecture is produced to support dynamic Web services. These architectures are combined to produce CREWS, a Component-driven Runtime Extensible Web Service solution that enables Web services to support the ever changing needs of enterprises. A discussion of similar work is presented, identifying the strengths and weaknesses of our architecture when compared to other solutions.
- Full Text:
Decryption of Wire-level Network Protocols for Forensic Inspection
- Authors: Irwin, Barry V W
- Date: 2004
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/428339 , vital:72504 , https://www.researchgate.net/profile/Barry-Irwin/publication/327622612_Decryption_of_Wire-lev-el_Network_Protocols_for_Forensic_Inspection/links/5b9a1220299bf14ad4d6a3b1/Decryption-of-Wire-level-Network-Protocols-for-Forensic-Inspection.pdf
- Description: With the increased use of encrypted transport protocols, the problem of debugging and monitoring the contents of these protocols has in-creased in complexity. This work proposes the development of a unified means of access to the plaintext, through the use of privileged access to the encryption keys, based on the assumption that an administrator has legitimate access to one side of a communication, and is thereby able to gain access to the encryption tokens.
- Full Text:
Design and evaluation of a new pharmaceutical pictogram sequence to convey medicine usage
- Authors: Mansoor, Leila E , Dowse, Roslind
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6402 , http://hdl.handle.net/10962/d1006338
- Description: Pictorials may be used to augment textual instructions in the depiction of safety and warning information on medicines. The objective of this study was to design, develop and evaluate a simple and culturally appropriate pictogram sequence for using nystatin suspension, and to assess its understandability in low-literate Xhosa participants. A new pharmaceutical pictogram sequence was designed through focus group discussions and evaluated in a 2-phase process. The results of Phase 1 (30 participants) identified various problems associated with the new pictogram sequence. It was modified accordingly and re-evaluated in Phase 2 with 20 participants. All participants belonged to the Xhosa group, had between 0 and 7 years of formal schooling and had English as their second language. Acceptance of the new pictogram sequence was based on international standards (ANSI and ISO criterion) for evaluating the comprehensibility of pictograms. In Phase 1, the new pictogram sequence was correctly interpreted by 66.7% of the participants and this complied with the ISO criterion of 67% correct. In Phase 2, 95% of the participants were able to correctly interpret the new pictogram sequence. This result complied with the ANSI criterion of 85% correct, therefore, this new pictogram sequence was considered to be acceptable. This study has illustrated the success of using a consultative approach in the design of new pictograms.
- Full Text:
Design of a performance evaluation tool for multimedia databases with special reference to Oracle
- Authors: Stakemire, Tonia
- Date: 2004
- Subjects: Oracle (Computer file) , Application software -- Evaluation , Database management -- Evaluation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4705 , http://hdl.handle.net/10962/d1015975
- Description: Increased production and use of multimedia data has led to the development of a more advanced Database Management System (DBMS), like an Object Relational Database Management System (ORDBMS). These advanced databases are necessitated by the complexity in structure and the functionality required by multimedia data. Unfortunately, no suitable benchmarks exist with which to test the performance of databases when handling multimedia data. This thesis describes the design of a benchmark to measure the performance of basic functionality found in multimedia databases. The benchmark, called MORD (Multimedia Object Relational Databases), targets Oracle, a well known commercial Object Relational Database Management System (ORDBMS) that can handle multimedia data. Although MORD targets Oracle, it can easily be applied to other Multimedia Database Management System (MMDBMS) as a result of a design that stressed its portability, and simplicity. MORD consists of a database schema, test data, and code to simulate representative queries on multimedia databases. A number of experiments are described that validate MORD and ensure its correct design and that its objectives are met. A by-product of these experiments is an initial understanding of the performance of multimedia databases. The experiments show that with multimedia data the buffer cache should be at least large enough to hold the largest dataset, a bigger block size improves the performance, and turning off logging and caching for bulk loading improves the performance. MORD can be used to compare different ORDBMS or to assist in the configuration of a specific database.
- Full Text:
Determination of 2′, 3′-Dideoxyinosine Using Iron (II) Phthalocyanine Modified Carbon Paste Electrode
- Authors: Ozoemena, Kenneth I , Stefan, Raluca-Ioana , Nyokong, Tebello
- Date: 2004
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/300298 , vital:57914 , xlink:href="https://doi.org/10.1081/AL-200031940"
- Description: An amperometric sensor, based on carbon paste impregnated with iron (II) phthalocyanine complex (FePc), has been constructed for the assay of anti‐HIV agent dideoxyinosine (didanosine, DDI). Using chronoamperometry (E = +1.04 V versus Ag/AgCl) technique, the amperometric sensor can be used reliably for dideoxyinosine assay at pH 7.4 phosphate buffer in the 1.5–9.5 nmol/L concentration range with a detection limit of 5.7 × 10−10 mol/L. The surface of the electrode can easily be regenerated by simple polishing, obtaining a fresh surface ready for use in a new assay. The new amperometric sensor proved to be highly reliable for the assay of dideoxyinosine purity in raw material as well as for the uniformity content test of Videx® tablets.
- Full Text:
Developing evidence-based practice: the role of case-based research
- Authors: Edwards, David J A , Dattilio, F M , Bromley, D B
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6243 , http://hdl.handle.net/10962/d1007856
- Description: How can practitioners engage in evidence-based practice when the evidence for effectiveness of psychological treatments comes from randomized controlled trials using patient populations different from those encountered in everyday settings and treatment manuals that seem oversimplified and inflexible? The authors argue that important evidence about best practice comes from case-based research, which builds knowledge in a clinically useful manner and complements what is achieved by multivariate research methods. A multidimensional model of the research process is provided that includes clinical practice and case-based research as significant contributors. The authors summarize the principles of case-based research and provide examples of recent technical advances. Finally, the authors suggest ways in which practitioners can apply the case-based approach in researching and publishing their own cases, perhaps in collaboration with university-based researchers.
- Full Text:
Development of a hydantoin-hydrolysing biocatalyst for the production of optically pure amino acids using Agrobacterium tumefaciens strain RU-ORPN1
- Authors: Foster, Ingrid Margaret
- Date: 2004
- Subjects: Agrobacterium tumefaciens Amino acids Hydantoin Hydrolysis Enzymes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3943 , http://hdl.handle.net/10962/d1004002
- Description: A calcium alginate bead-immobilised biocatalyst was developed utilising the D-hydantoinase and D-N-carbamoylase from a novel, mutant Agrobacterium tumefaciens strain RU-ORPN1. The growth conditions for the inducer-independent strain were optimised for production of hydantoinase and N-carbamoylase activities. Methods for the preparation of crude enzyme extracts were evaluated in terms of hydantoinase and N-carbamoylase activities produced. After comparison of the enzyme activities and stabilities in various extracts from fresh and frozen cells, sonication of frozen cells for 5 minutes was found to be the best method for the production of the enzyme extract. The optimal pH and temperature for the hydantoinase activity were pH 10 and 30°C, respectively, while pH 9 and 40°C were optimal for Ncarbamoylase activity. The hydantoinase activity was enhanced by the addition of Mg^(2+) ions to the enzyme extract and the N-carbamoylase was enhanced by the addition of Mg^(2+), Mn^(2+) or Zn^(2+) ions to the enzyme extract. The enzyme activities increased in the presence of ATP suggesting that the enzymes may be ATP-dependent. The addition of DTT and PMSF to the enzyme extract enhanced the hydantoinase activity but had no effect on the N-carbamoylase activity. The N-carbamoylase was unstable at 40°C and was almost completely inactivated after 24 hours incubation at this temperature. The hydantoinase and N-carbamoylase appeared to be insoluble. Various techniques were investigated for the solubilisation of the enzymes including various cell lysis methods, cell lysis at extremes of pH and ionic strength, addition of a reducing agent and protease inhibitors, and treatment with hydrolysing enzymes and detergents. Treatment with Triton X-100 was most effective for the solubilisation of the enzymes indicating that the enzymes were membrane-bound. Hydropathy and transmembrane prediction plots of the predicted amino acid sequences for two identified N-carbamoylase genes from A. tumefaciens RU-ORPN1 revealed possible transmembrane regions in the amino acid sequences, and thus supported the hypothesis that the enzymes were membrane-bound. Various methods were evaluated for the immobilisation of the enzymes in whole cells and enzyme extracts. Immobilisation of the enzyme extract in calcium alginate beads was found to be the best method in terms of enzyme activity retention and stability. The hydantoinase retained 55% activity while the N-carbamoylase exhibited a remarkable sevenfold increase in activity after immobilisation by this method. Furthermore, the hydantoinase activity increased after storage at 4°C for 21 days, while the N-carbamoylase retained 30% activity after this storage period. The calcium alginate bead-immobilised enzymes were further biochemically characterised and then applied in a bioreactor system for the production of D-hydroxyphenylglycine (D-HPG) from D,L-5-hydroxyphenylhydantoin (D,L-5-HPH). The pH and temperature optima for the immobilised hydantoinase were pH 7 and 50°C, respectively, while pH 8 and 40°C were optimal for the immobilised N-carbamoylase enzyme. The immobilised enzymes showed improved thermostability at 40°C in comparison to the free enzymes and retained high levels of activity after five repeated batch reactions. Low levels of conversion were obtained in a packed-bed bioreactor containing the A. tumefaciens RU-ORPN1 biocatalyst due to the low hydantoinase activity present in the strain, relative to N-carbamoylase. A novel, packed-bed bioreactor system was therefore developed for the production of D-HPG from D,L-5-HPH using the A. tumefaciens biocatalyst in combination with a Pseudomonas sp. biocatalyst having high hydantoinase activity. A conversion yield of 22 to 30% was achieved for the production of D-HPG from D,L-5-HPH over 5 days operation demonstrating that the hydantoin-hydrolysing enzymes from A. tumefaciens RU-ORPN1 could be stabilised by immobilisation and, in combination with a biocatalyst with high hydantoinase activity, could be applied to the fully enzymatic conversion of D,L-5-HPH to D-HPG.
- Full Text:
Discourse domination?: the role of gender in seminar interaction
- Authors: De Klerk, Vivian A , Hunt, Sally
- Date: 2004
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/139160 , vital:37710 , https://www.ajol.info/index.php/salas/article/view/6501
- Description: This paper focuses on the effect of the gender of participants on the discourse patterns of university seminars, and compares the interaction patterns in two undergraduate seminars with those in two post-graduate seminars at Rhodes University. In the undergraduate seminars, two different groups of students at first year level in different disciplines were videotaped. The major difference was in terms of composition: in one seminar, there were equal numbers of male and female students, while in the other, female students dominated numerically (75%). In addition, five of the six formed a close-knit group of friends, which proved to be an important factor in the analysis. At the postgraduate level, the student participants were identical and met in the same venue and at the same time on two successive days to discuss the same topic (affirmative action in the workplace). The important difference between the two classes was the fact that the first seminar was led by a female tutor and the second seminar by a male tutor, both under 40 years of age and white, both members of staff in the department, and both known to the students. The discourse patterns in these two sets of seminars were analysed in order to investigate the ways in which the gender of tutors and students influenced the floor-winning and floor-holding conventions of participants.
- Full Text:
Distinct kimberlite pipe classes with contrasting eruption processes
- Authors: Skinner, E M W , Marsh, Julian S
- Date: 2004
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/150603 , vital:38988 , https://doi.org/10.1016/j.lithos.2004.03.044
- Description: Field and Scott Smith [Field, M., Scott Smith, B.H., 1999. Contrasting geology and near-surface emplacement of kimberlite pipes in southern Africa and Canada. Proc. 7th Int. Kimb. Conf. (Eds. Gurney et al.) 1, 214–237.] propose that kimberlite pipes can be grouped into three types or classes. Classical or Class 1 pipes are the only class with characteristic low temperature, diatreme-facies kimberlite in addition to hypabyssal- and crater-facies kimberlite. Class 2 and 3 pipes are characterized only by hypabyssal-and crater-facies kimberlite.
- Full Text: false
Drimia chalumnensis (Hyacinthaceae - Urgineoideae), a new species from Eastern Cape, South Africa
- Authors: Dold, Anthony P , Brink, Estelle
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6516 , http://hdl.handle.net/10962/d1005944
- Description: Drimia chalumnensis, a new inconspicuous dwarf species from the Albany Centre of Floristic Endemism in Eastern Cape, South Africa, is restricted to the coastal plains of the Chalumna river in small scattered aggregated colonies on exposed sandstone sheets in pockets of loose sandy soil. It is distinguished by its capitate inflorescence, separate, petiolate bulb scales, and prostrate, coriaceous, linear-lanceolate leaves with cartilaginous margins.
- Full Text:
DRUBIS : a distributed face-identification experimentation framework - design, implementation and performance issues
- Authors: Ndlangisa, Mboneli
- Date: 2004
- Subjects: Principal components analysis , Human face recognition (Computer science) , Image processing , Biometric identification
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4704 , http://hdl.handle.net/10962/d1015768
- Description: We report on the design, implementation and performance issues of the DRUBIS (Distributed Rhodes University Biometric Identification System) experimentation framework. The Principal Component Analysis (PCA) face-recognition approach is used as a case study. DRUBIS is a flexible experimentation framework, distributed over a number of modules that are easily pluggable and swappable, allowing for the easy construction of prototype systems. Web services are the logical means of distributing DRUBIS components and a number of prototype applications have been implemented from this framework. Different popular PCA face-recognition related experiments were used to evaluate our experimentation framework. We extract recognition performance measures from these experiments. In particular, we use the framework for a more indepth study of the suitability of the DFFS (Difference From Face Space) metric as a means for image classification in the area of race and gender determination.
- Full Text:
Early development of water quality methods and approaches in ecological Reserve assessments
- Authors: Palmer, Carolyn G , Scherman, Patricia , Muller, Nikite W J , Rossouw, J N , Malan, H L , Jooste, S
- Date: 2004
- Subjects: To be catalogued
- Language: English
- Type: text , report
- Identifier: http://hdl.handle.net/10962/437840 , vital:73416 , ISBN 1-77005-227-5 , https://wrcwebsite.azurewebsites.net/wp-content/uploads/mdocs/1108-1-041.pdf
- Description: This project was initiated in 2000 to allow the ecological Reserve team for water quality to undertake additional research while working on eco-logical Reserve determinations funded by the Department of Water Af-fairs and Forestry. The results of this work form the basis of the meth-ods reported in the Water Research Commission report Hughes DA (Ed)(in press).
- Full Text:
Economic feasibility of an experimental octopus fishery in South Africa
- Authors: Oosthuizen, Ané
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6760 , http://hdl.handle.net/10962/d1007773
- Description: Octopus vulgaris was identified as a new marine resource to be commercially exploited through an experimental fishery. A recent policy on developing fisheries in South Africa emphasizes the importance of investigating the economic feasibility of a fishery as part of its management framework. The study reported here generated baseline information necessary in the design of the experimental fishery, giving guidelines as to which vessels, fishing gear and markets would be most feasible. The proposed fishery, gear and vessel type, fishing techniques and expected catch rates are described, the results of market research are outlined, and the cost of fishing is estimated. The potential business should consist of small and medium-sized vessels deploying unbaited pots attached to long lines. The longline pot fishery could be economically feasible, provided a 30 percent catch in 6600 pots/month is attained. Only existing, debt-free vessels should be used. The minimum catch per unit effort (CPUE) for various fishing operation scenarios was calculated to determine economic feasibility. This is an estimated minimum CPUE, based on assumptions that cannot be confirmed until the fishery starts. Furthermore, this economic analysis also needs to be assessed by stakeholders with experience of fishery operations , Rhodes Centenary issue of: South African Journal of Science.
- Full Text:
Effects of substituents and solvents on the photochemical properties of zinc phthalocyanine complexes and their protonated derivatives
- Authors: Ogunsipe, Abimbola Olukayode , Nyokong, Tebello
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6585 , http://hdl.handle.net/10962/d1004161
- Description: Zinc phthalocyanine derivatives containing various ring substituents and axial ligands were studied with respect to the effect of substituents on protonation. Aggregation resulted in failure of some of the ZnPc derivatives to protonate. In fact addition of protonating agents to aggregated ZnPc derivatives resulted in increase in aggregation followed by slow degradation. Axial ligands were lost on protonation. Photobleaching, fluorescence and singlet oxygen quantum yields of the protonated derivatives were compared with those of the unprotonated derivatives. In all cases protonation decreases the singlet oxygen and fluorescence quantum yields. However, photobleaching quantum yields decreased (i.e. stability increased) for the protonated derivatives except when axial ligands were present, where protonation resulted in decrease in stability.
- Full Text: