Gold nanoparticle–based colorimetric probes for dopamine detection
- Authors: Ngomane, Nokuthula
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4565 , http://hdl.handle.net/10962/d1021261
- Description: Colorimetric probes have become important tools in analysis and biomedical technology. This thesis reports on the development of such probes for the detection of dopamine (DA). Liquid and different solid state probes were developed utilizing un–functionalized gold nanoparticles (UF–AuNPs). The liquid state probe is based on the growth and aggregation of the UF–AuNPs in the presence of DA. Upon addition of the UF–AuNPs to various concentrations of DA, the shape, size and colour change results in spectral shifts from lower to higher wavelengths. The analyte can be easily monitored by the naked eye from 5.0 nM DA with a calculated limit of detection of 2.5 nM (3σ) under optimal pH. Ascorbic acid (AA) has a potential to interfere with DA detection in solution since it is often present in biological fluids, but in this case the interference was limited to solutions where its concentration was beyond 200 times greater than that of DA. Since most of the previously reported colorimetric probes, especially those for DA are solution based, the main focus of the thesis was in the development of a solid state based colorimetric probe in the form of nanofibre mats. To overcome the interference challenges experienced in the solution studies (the interference by high concentrations of AA), the suitability of molecularly imprinted polymers (MIPs) for the selective detection of DA was investigated. The results showed that the MIPs produced did not play a significant role in enhancing the selectivity towards DA. A probe composed of just the UF–AuNPs and Nylon–6 (UF–AuNPs + N6) was also developed. The UF–AuNPs were synthesized following an in situ reduction method. The probe was only selective to DA and insensitive to other catecholamines at physiological pH. Thus, the probe did not require any addition functionalities to achieve selectivity and sensitive to DA. The liquid state probe and the composite UF–AuNPs + N6 nanofibre probe were successfully applied to a whole blood sample and showed good selectivity towards DA. The simple, sensitive and selective probe could be an excellent alternative for on–site and immediate detection of DA without the use of instrumentation. For quantification of DA using the solid state probe, open–source software imageJ was used to assist in the analysis of the nanofibre colours. It was observed that the intensity of the colour increased with the increase in concentration of DA in a linear fashion. The use of imageJ can also be a great alternative where the colour changes are not so clear or for visually impaired people. The solid state probe developed can detect DA qualitatively and quantitatively. The work also forms a good foundation for development of such probes for other analyte.
- Full Text:
- Authors: Ngomane, Nokuthula
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4565 , http://hdl.handle.net/10962/d1021261
- Description: Colorimetric probes have become important tools in analysis and biomedical technology. This thesis reports on the development of such probes for the detection of dopamine (DA). Liquid and different solid state probes were developed utilizing un–functionalized gold nanoparticles (UF–AuNPs). The liquid state probe is based on the growth and aggregation of the UF–AuNPs in the presence of DA. Upon addition of the UF–AuNPs to various concentrations of DA, the shape, size and colour change results in spectral shifts from lower to higher wavelengths. The analyte can be easily monitored by the naked eye from 5.0 nM DA with a calculated limit of detection of 2.5 nM (3σ) under optimal pH. Ascorbic acid (AA) has a potential to interfere with DA detection in solution since it is often present in biological fluids, but in this case the interference was limited to solutions where its concentration was beyond 200 times greater than that of DA. Since most of the previously reported colorimetric probes, especially those for DA are solution based, the main focus of the thesis was in the development of a solid state based colorimetric probe in the form of nanofibre mats. To overcome the interference challenges experienced in the solution studies (the interference by high concentrations of AA), the suitability of molecularly imprinted polymers (MIPs) for the selective detection of DA was investigated. The results showed that the MIPs produced did not play a significant role in enhancing the selectivity towards DA. A probe composed of just the UF–AuNPs and Nylon–6 (UF–AuNPs + N6) was also developed. The UF–AuNPs were synthesized following an in situ reduction method. The probe was only selective to DA and insensitive to other catecholamines at physiological pH. Thus, the probe did not require any addition functionalities to achieve selectivity and sensitive to DA. The liquid state probe and the composite UF–AuNPs + N6 nanofibre probe were successfully applied to a whole blood sample and showed good selectivity towards DA. The simple, sensitive and selective probe could be an excellent alternative for on–site and immediate detection of DA without the use of instrumentation. For quantification of DA using the solid state probe, open–source software imageJ was used to assist in the analysis of the nanofibre colours. It was observed that the intensity of the colour increased with the increase in concentration of DA in a linear fashion. The use of imageJ can also be a great alternative where the colour changes are not so clear or for visually impaired people. The solid state probe developed can detect DA qualitatively and quantitatively. The work also forms a good foundation for development of such probes for other analyte.
- Full Text:
GPU Accelerated protocol analysis for large and long-term traffic traces
- Nottingham, Alastair Timothy
- Authors: Nottingham, Alastair Timothy
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/910 , vital:20002
- Description: This thesis describes the design and implementation of GPF+, a complete general packet classification system developed using Nvidia CUDA for Compute Capability 3.5+ GPUs. This system was developed with the aim of accelerating the analysis of arbitrary network protocols within network traffic traces using inexpensive, massively parallel commodity hardware. GPF+ and its supporting components are specifically intended to support the processing of large, long-term network packet traces such as those produced by network telescopes, which are currently difficult and time consuming to analyse. The GPF+ classifier is based on prior research in the field, which produced a prototype classifier called GPF, targeted at Compute Capability 1.3 GPUs. GPF+ greatly extends the GPF model, improving runtime flexibility and scalability, whilst maintaining high execution efficiency. GPF+ incorporates a compact, lightweight registerbased state machine that supports massively-parallel, multi-match filter predicate evaluation, as well as efficient arbitrary field extraction. GPF+ tracks packet composition during execution, and adjusts processing at runtime to avoid redundant memory transactions and unnecessary computation through warp-voting. GPF+ additionally incorporates a 128-bit in-thread cache, accelerated through register shuffling, to accelerate access to packet data in slow GPU global memory. GPF+ uses a high-level DSL to simplify protocol and filter creation, whilst better facilitating protocol reuse. The system is supported by a pipeline of multi-threaded high-performance host components, which communicate asynchronously through 0MQ messaging middleware to buffer, index, and dispatch packet data on the host system. The system was evaluated using high-end Kepler (Nvidia GTX Titan) and entry level Maxwell (Nvidia GTX 750) GPUs. The results of this evaluation showed high system performance, limited only by device side IO (600MBps) in all tests. GPF+ maintained high occupancy and device utilisation in all tests, without significant serialisation, and showed improved scaling to more complex filter sets. Results were used to visualise captures of up to 160 GB in seconds, and to extract and pre-filter captures small enough to be easily analysed in applications such as Wireshark.
- Full Text:
- Authors: Nottingham, Alastair Timothy
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/910 , vital:20002
- Description: This thesis describes the design and implementation of GPF+, a complete general packet classification system developed using Nvidia CUDA for Compute Capability 3.5+ GPUs. This system was developed with the aim of accelerating the analysis of arbitrary network protocols within network traffic traces using inexpensive, massively parallel commodity hardware. GPF+ and its supporting components are specifically intended to support the processing of large, long-term network packet traces such as those produced by network telescopes, which are currently difficult and time consuming to analyse. The GPF+ classifier is based on prior research in the field, which produced a prototype classifier called GPF, targeted at Compute Capability 1.3 GPUs. GPF+ greatly extends the GPF model, improving runtime flexibility and scalability, whilst maintaining high execution efficiency. GPF+ incorporates a compact, lightweight registerbased state machine that supports massively-parallel, multi-match filter predicate evaluation, as well as efficient arbitrary field extraction. GPF+ tracks packet composition during execution, and adjusts processing at runtime to avoid redundant memory transactions and unnecessary computation through warp-voting. GPF+ additionally incorporates a 128-bit in-thread cache, accelerated through register shuffling, to accelerate access to packet data in slow GPU global memory. GPF+ uses a high-level DSL to simplify protocol and filter creation, whilst better facilitating protocol reuse. The system is supported by a pipeline of multi-threaded high-performance host components, which communicate asynchronously through 0MQ messaging middleware to buffer, index, and dispatch packet data on the host system. The system was evaluated using high-end Kepler (Nvidia GTX Titan) and entry level Maxwell (Nvidia GTX 750) GPUs. The results of this evaluation showed high system performance, limited only by device side IO (600MBps) in all tests. GPF+ maintained high occupancy and device utilisation in all tests, without significant serialisation, and showed improved scaling to more complex filter sets. Results were used to visualise captures of up to 160 GB in seconds, and to extract and pre-filter captures small enough to be easily analysed in applications such as Wireshark.
- Full Text:
Grace “The story of a wise cat and a cruel man.”
- Authors: Phepheng, Maruping
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6005 , http://hdl.handle.net/10962/d1021213
- Description: Morgan, a widower and police detective, is quickly promoted from constable to captain after a series of successful criminal investigations, creating enemies in the process. One of the convicted and incarcerated is Paper, a gang leader with a violent upbringing. After a lucky release from prison, Paper, now Morgan’s fierce foe, plots to hurt him in the worst way possible – by killing his only son. The story is narrated by a cat named Grace, whose presence and companionship comes from another dimension and helps Morgan to keep sane.
- Full Text:
- Authors: Phepheng, Maruping
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6005 , http://hdl.handle.net/10962/d1021213
- Description: Morgan, a widower and police detective, is quickly promoted from constable to captain after a series of successful criminal investigations, creating enemies in the process. One of the convicted and incarcerated is Paper, a gang leader with a violent upbringing. After a lucky release from prison, Paper, now Morgan’s fierce foe, plots to hurt him in the worst way possible – by killing his only son. The story is narrated by a cat named Grace, whose presence and companionship comes from another dimension and helps Morgan to keep sane.
- Full Text:
Gradient high performance liquid chromatographic method for the simultaneous analysis of efavirenz, emtricitabine and tenofovir
- Authors: Koekemoer, Sonya Mariana
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/54679 , vital:26599
- Description: In 2014, approximately 6.8 million people in South Africa were HIV-positive, and the majority of those affected are aged 15 or older. A fixed-dose combination (FDC) antiretroviral (ARV) dosage form containing one non-nucleotide reverse transcriptase inhibitor (efavirenz) and two nucleotide reverse transcriptase inhibitors (emtricitabine and tenofovir) was licensed in South Africa in April 2013. New consolidated guidelines for HIV management and prevention of mother to child transmission (PMTCT) were published by the South African Department of Health in December 2014 and the FDC is now the recommended first-line treatment for HIV-positive patients. According to these guidelines all such people aged 15 and older, and weighing more than 40 kg, with a CD4 count of ≤ 500/ μl will be eligible for antiretroviral therapy (ART) using the FDC. In addition every pregnant and breastfeeding woman is eligible for lifelong ART regardless of CD4 count and EFV can be used as first-line treatment for pregnant women regardless of the length of gestation state of the pregnancy at that time. The use of this simplified regime is likely to promote much needed and improved adherence to therapy. An investigation into the development of a stability-indicating reversed-phase high performance liquid chromatography (RP-HPLC) method for the simultaneous quantitation of EFV, FTC and TNF was undertaken. Isocratic HPLC analysis was found to be unsuitable due to the highly polar FTC molecule eluting in the void. Therefore a gradient HPLC method was developed and validated. The method was validated according to the International Conference on Harmonisation, now known as International Council for Harmonization (ICH). Correlation coefficients > 0.999 were obtained for each assessment of linearity and FTC, TNF and EFV are linear in the range 0.4-40 μg/ml, 0.6-60 μg/ml and 1.2-120 μg/ml. The equation of the best-fit least squares regression lines for FTC, TNF and EFV were y = 0.0191x+0.0007, y = 0.0163x+0.0116 and y = 0.01x+0.016, respectively. The method is accurate as the y-intercept was < 2% of the detector response for all ARV, and the method is precise in terms of intra- and inter-assay precision as all % RSD < 2%. The stability-indicating nature of the method was demonstrated under acidic, alkaline and oxidative stress in addition to UV exposure and elevated temperatures, and the individual chromatograms were overlaid using Empower® 3 Software to establish whether there was interference with the peaks of interest. The forced degradation studies demonstrated the selectivity of the method for the ARV compounds. The method was applied to assay and in vitro dissolution studies of commercially available tablets. The amount of each active ingredient released from Atripla® was determined and compared to the amount of each drug released from Aspen Efavirenz® and Truvada® (a combination of FTC and TNF). The percent FTC released from Atripla® and Truvada® was similar based on the acceptance criteria for immediate-release BCS class 1 compounds. Statistical analysis was undertaken to compare the dissolution profiles of FTC, TNF and EFV. The percent of these compounds released in these studies indicate that bioequivalence testing would be required to declare these products interchangeable. The validated RP-HPLC and in vitro dissolution test method are suitable for routine quality control testing of solid oral dosage forms containing EFV, FTC and TNF, and as the dissolution method can discriminate between different formulations of the same molecule, these tools can also be used for analysis during formulation development studies. The method is not suitable for the analysis of the ARV plasma due to lack of sensitivity and an inability to quantitate the compounds at the required concentration levels. The use of HPLC with mass spectroscopy for quantitation would enhance the sensitivity of the method and may eliminate the quantitation of the molecules in the presence of interference that was observed when using UV detection. Fixed dose combination tablets are convenient for patient therapy and it is likely that in the future more molecules will be formulated into such dosage forms. However formulations such as these can pose significant difficulties when developing and using analytical methods for the quantitation of all compounds in the dosage form at the same time, in particular when the compounds have vastly different physico-chemical properties that impact the quality of a separation and therefore the analysis. Therefore when embarking on the development of FDC product cognisance of the difficulties of developing single methods for the analyses is required and approaches to overcome these difficulties should be considered.
- Full Text:
- Authors: Koekemoer, Sonya Mariana
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/54679 , vital:26599
- Description: In 2014, approximately 6.8 million people in South Africa were HIV-positive, and the majority of those affected are aged 15 or older. A fixed-dose combination (FDC) antiretroviral (ARV) dosage form containing one non-nucleotide reverse transcriptase inhibitor (efavirenz) and two nucleotide reverse transcriptase inhibitors (emtricitabine and tenofovir) was licensed in South Africa in April 2013. New consolidated guidelines for HIV management and prevention of mother to child transmission (PMTCT) were published by the South African Department of Health in December 2014 and the FDC is now the recommended first-line treatment for HIV-positive patients. According to these guidelines all such people aged 15 and older, and weighing more than 40 kg, with a CD4 count of ≤ 500/ μl will be eligible for antiretroviral therapy (ART) using the FDC. In addition every pregnant and breastfeeding woman is eligible for lifelong ART regardless of CD4 count and EFV can be used as first-line treatment for pregnant women regardless of the length of gestation state of the pregnancy at that time. The use of this simplified regime is likely to promote much needed and improved adherence to therapy. An investigation into the development of a stability-indicating reversed-phase high performance liquid chromatography (RP-HPLC) method for the simultaneous quantitation of EFV, FTC and TNF was undertaken. Isocratic HPLC analysis was found to be unsuitable due to the highly polar FTC molecule eluting in the void. Therefore a gradient HPLC method was developed and validated. The method was validated according to the International Conference on Harmonisation, now known as International Council for Harmonization (ICH). Correlation coefficients > 0.999 were obtained for each assessment of linearity and FTC, TNF and EFV are linear in the range 0.4-40 μg/ml, 0.6-60 μg/ml and 1.2-120 μg/ml. The equation of the best-fit least squares regression lines for FTC, TNF and EFV were y = 0.0191x+0.0007, y = 0.0163x+0.0116 and y = 0.01x+0.016, respectively. The method is accurate as the y-intercept was < 2% of the detector response for all ARV, and the method is precise in terms of intra- and inter-assay precision as all % RSD < 2%. The stability-indicating nature of the method was demonstrated under acidic, alkaline and oxidative stress in addition to UV exposure and elevated temperatures, and the individual chromatograms were overlaid using Empower® 3 Software to establish whether there was interference with the peaks of interest. The forced degradation studies demonstrated the selectivity of the method for the ARV compounds. The method was applied to assay and in vitro dissolution studies of commercially available tablets. The amount of each active ingredient released from Atripla® was determined and compared to the amount of each drug released from Aspen Efavirenz® and Truvada® (a combination of FTC and TNF). The percent FTC released from Atripla® and Truvada® was similar based on the acceptance criteria for immediate-release BCS class 1 compounds. Statistical analysis was undertaken to compare the dissolution profiles of FTC, TNF and EFV. The percent of these compounds released in these studies indicate that bioequivalence testing would be required to declare these products interchangeable. The validated RP-HPLC and in vitro dissolution test method are suitable for routine quality control testing of solid oral dosage forms containing EFV, FTC and TNF, and as the dissolution method can discriminate between different formulations of the same molecule, these tools can also be used for analysis during formulation development studies. The method is not suitable for the analysis of the ARV plasma due to lack of sensitivity and an inability to quantitate the compounds at the required concentration levels. The use of HPLC with mass spectroscopy for quantitation would enhance the sensitivity of the method and may eliminate the quantitation of the molecules in the presence of interference that was observed when using UV detection. Fixed dose combination tablets are convenient for patient therapy and it is likely that in the future more molecules will be formulated into such dosage forms. However formulations such as these can pose significant difficulties when developing and using analytical methods for the quantitation of all compounds in the dosage form at the same time, in particular when the compounds have vastly different physico-chemical properties that impact the quality of a separation and therefore the analysis. Therefore when embarking on the development of FDC product cognisance of the difficulties of developing single methods for the analyses is required and approaches to overcome these difficulties should be considered.
- Full Text:
Graphene quantum dots functionalized with 4-amino-2, 2, 6, 6-tetramethylpiperidine-N-oxide as fluorescence “turn-on” nanosensors
- Achadu, Ojodomo J, Britton, Jonathan, Nyokong, Tebello
- Authors: Achadu, Ojodomo J , Britton, Jonathan , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/239854 , vital:50773 , xlink:href="https://doi.org/10.1007/s10895-016-1916-y"
- Description: In this study, we report on the fabrication of simple and rapid graphene quantum dots (GQDs)-based fluorescence “turn-ON” nanoprobes for sensitive and selective detection of ascorbic acid (AA). Pristine GQDs and S and N co-doped-GQDs (SN-GQDs) were functionalized with 4-amino-2,2,6,6-tetramethylpiperidine-N-oxide (4-amino-TEMPO, a nitroxide free radical). The nitroxide free radicals efficiently quenched the fluorescence of the GQDs and upon interaction of the nanoconjugates with ascorbic acid, the quenched fluorescence was restored. The linear ranges recorded were 0.5–5.7 μM and 0.1–5.5 μM for GQDs-4-amino-TEMPO and SN-GQDs-4amino-TEMPO nanoprobes, respectively. Limits of detection were found to be 60 nM and 84 nM for SN-GQDS-4-amino-TEMPO and GQDs-4-amino-TEMPO for AA detection, respectively. This novel fluorescence “turn-ON” technique showed to be highly rapid and selective towards AA detection.
- Full Text:
- Authors: Achadu, Ojodomo J , Britton, Jonathan , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/239854 , vital:50773 , xlink:href="https://doi.org/10.1007/s10895-016-1916-y"
- Description: In this study, we report on the fabrication of simple and rapid graphene quantum dots (GQDs)-based fluorescence “turn-ON” nanoprobes for sensitive and selective detection of ascorbic acid (AA). Pristine GQDs and S and N co-doped-GQDs (SN-GQDs) were functionalized with 4-amino-2,2,6,6-tetramethylpiperidine-N-oxide (4-amino-TEMPO, a nitroxide free radical). The nitroxide free radicals efficiently quenched the fluorescence of the GQDs and upon interaction of the nanoconjugates with ascorbic acid, the quenched fluorescence was restored. The linear ranges recorded were 0.5–5.7 μM and 0.1–5.5 μM for GQDs-4-amino-TEMPO and SN-GQDs-4amino-TEMPO nanoprobes, respectively. Limits of detection were found to be 60 nM and 84 nM for SN-GQDS-4-amino-TEMPO and GQDs-4-amino-TEMPO for AA detection, respectively. This novel fluorescence “turn-ON” technique showed to be highly rapid and selective towards AA detection.
- Full Text:
HealthMessenger: an e-Health service to support ICT deployments in poor areas
- Gremu, Chikumbutso, Terzoli, Alfredo, Tsietsi, Mosiuoa
- Authors: Gremu, Chikumbutso , Terzoli, Alfredo , Tsietsi, Mosiuoa
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/430609 , vital:72703 , 10.1109/ISTAFRICA.2016.7530632
- Description: Development and implementation of appropriate e-services as well as revenue generation are key to deploying and sustaining ICT installa-tions in poor areas in developing countries. The area of e-Health is a promising area for e-Services that are important to populations in poor areas and health service organisations that are already spending mon-ey on different health initiatives in these areas. This paper discusses an e-Health service that facilitates dissemination of health information to people living in poor areas and that is subsequently used to generate revenue to support deployment and development of ICT in the areas. The tool was developed within the context of the Siyakhula Living Lab (SLL), a multi-stakeholder operation that promotes ICT for Development in the Eastern Cape Province of South Africa. Creation of health con-tent for the e-health service and its subsequent consumption happen in two different environments from a technological and social point of view. To enable this to happen, the e-service comprises two component applications that run in a peer-to-peer fashion. The component that is used to disseminate content for end-user consumption is called the HealthMessenger. The HealthMessenger is hosted on an environment called TeleWeaver, an application integration platform developed within the SLL to host software applications targeting people living in poor ar-eas. The platform is customised to support services with a revenue generation component.
- Full Text:
- Authors: Gremu, Chikumbutso , Terzoli, Alfredo , Tsietsi, Mosiuoa
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/430609 , vital:72703 , 10.1109/ISTAFRICA.2016.7530632
- Description: Development and implementation of appropriate e-services as well as revenue generation are key to deploying and sustaining ICT installa-tions in poor areas in developing countries. The area of e-Health is a promising area for e-Services that are important to populations in poor areas and health service organisations that are already spending mon-ey on different health initiatives in these areas. This paper discusses an e-Health service that facilitates dissemination of health information to people living in poor areas and that is subsequently used to generate revenue to support deployment and development of ICT in the areas. The tool was developed within the context of the Siyakhula Living Lab (SLL), a multi-stakeholder operation that promotes ICT for Development in the Eastern Cape Province of South Africa. Creation of health con-tent for the e-health service and its subsequent consumption happen in two different environments from a technological and social point of view. To enable this to happen, the e-service comprises two component applications that run in a peer-to-peer fashion. The component that is used to disseminate content for end-user consumption is called the HealthMessenger. The HealthMessenger is hosted on an environment called TeleWeaver, an application integration platform developed within the SLL to host software applications targeting people living in poor ar-eas. The platform is customised to support services with a revenue generation component.
- Full Text:
Hearing things
- Authors: Claassen, José
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6019 , http://hdl.handle.net/10962/d1021243
- Description: William Carlos Williams wrote: "It isn't what the poet says that counts as a work of art, it's what he makes, with such intensity of purpose that it lives with an intrinsic movement of its own to verify its authenticity." I would like to think that my poems only borrow life from my pen, taking on an identity and music of their own with the help of some ‘making’ on my part. My poems embrace a continuum of human experience from the intrapersonal to the societal. Using imagistic and cinematic forms, they preserve the vitality of their sources, from the music of cityscapes, to the texture of emotions, to the narratives of particular characters.
- Full Text:
- Authors: Claassen, José
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6019 , http://hdl.handle.net/10962/d1021243
- Description: William Carlos Williams wrote: "It isn't what the poet says that counts as a work of art, it's what he makes, with such intensity of purpose that it lives with an intrinsic movement of its own to verify its authenticity." I would like to think that my poems only borrow life from my pen, taking on an identity and music of their own with the help of some ‘making’ on my part. My poems embrace a continuum of human experience from the intrapersonal to the societal. Using imagistic and cinematic forms, they preserve the vitality of their sources, from the music of cityscapes, to the texture of emotions, to the narratives of particular characters.
- Full Text:
Heat accumulation and development rate of massed maggots of the sheep blowfly, Lucilia cuprina (Diptera: Calliphoridae)
- Kotzé, Zanthé, Villet, Martin H, Weldon, Christopher W
- Authors: Kotzé, Zanthé , Villet, Martin H , Weldon, Christopher W
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442168 , vital:73964 , https://doi.org/10.1016/j.jinsphys.2016.09.009
- Description: Blowfly larvae aggregate on exposed carcasses and corpses and pass through three instars before wandering from the carcass and pupating. The developmental landmarks in this process can be used by forensic entomologists to estimate the time since the insects colonised the carcass, which sets a minimum post mortem interval. Large aggregations of feeding larvae generate a microclimate with temperatures up to 15 °C above ambient conditions, which may accelerate larval development and affect forensic estimates of post-mortem intervals. This study investigated the effects of heat accumulated by maggot masses of Lucilia cuprina at aggregations of 20, 50 and 100 larvae, each at incubation temperatures of 18 °C, 24 °C and 30 °C, using body length and life stage as developmental indicators. Aggregation temperatures reached up to 18.7 °C above ambient temperature, with significant effects of both size and temperature of the aggregation on the development time of its larvae. Survivorship was highest for all life stages at 24 °C, which is near the developmental optimum of L. cuprina. The results of this study provide a broadly applicable method of quantifying heat accumulation by aggregations of a wide range of species of forensic importance, and the results obtained from such studies will demonstrate that ambient temperature cannot be considered the only source of heat that blowfly larvae experience when they develop on a carcass. Neglect of temperatures within larval aggregations will result in an overestimation of post-mortem intervals and thus have far-reaching medicolegal consequences.
- Full Text:
- Authors: Kotzé, Zanthé , Villet, Martin H , Weldon, Christopher W
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442168 , vital:73964 , https://doi.org/10.1016/j.jinsphys.2016.09.009
- Description: Blowfly larvae aggregate on exposed carcasses and corpses and pass through three instars before wandering from the carcass and pupating. The developmental landmarks in this process can be used by forensic entomologists to estimate the time since the insects colonised the carcass, which sets a minimum post mortem interval. Large aggregations of feeding larvae generate a microclimate with temperatures up to 15 °C above ambient conditions, which may accelerate larval development and affect forensic estimates of post-mortem intervals. This study investigated the effects of heat accumulated by maggot masses of Lucilia cuprina at aggregations of 20, 50 and 100 larvae, each at incubation temperatures of 18 °C, 24 °C and 30 °C, using body length and life stage as developmental indicators. Aggregation temperatures reached up to 18.7 °C above ambient temperature, with significant effects of both size and temperature of the aggregation on the development time of its larvae. Survivorship was highest for all life stages at 24 °C, which is near the developmental optimum of L. cuprina. The results of this study provide a broadly applicable method of quantifying heat accumulation by aggregations of a wide range of species of forensic importance, and the results obtained from such studies will demonstrate that ambient temperature cannot be considered the only source of heat that blowfly larvae experience when they develop on a carcass. Neglect of temperatures within larval aggregations will result in an overestimation of post-mortem intervals and thus have far-reaching medicolegal consequences.
- Full Text:
Heat shock protein inhibitors: success stories
- McAlpine, Shelli R, Edkins, Adrienne L
- Authors: McAlpine, Shelli R , Edkins, Adrienne L
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66359 , vital:28940 , https://doi.org/10.1007/978-3-319-32607-8
- Description: publisher version , Introduction: Medicinal chemistry is both science and art. The science of medicinal chemistry offers mankind one of its best hopes for improving the quality of life. The art of medicinal chemistry continues to challenge its practitioners with the need for both intuition and experience to discover new drugs. Hence sharing the experience of drug research is uniquely beneficial to the field of medicinal chemistry. Drug research requires interdisciplinary team-work at the interface between chemistry, biology and medicine. Therefore, the topic-related series Topics in Medicinal Chemistry covers all relevant aspects of drug research, e.g. pathobiochemistry of diseases, identification and validation of (emerging) drug targets, structural biology, drugability of targets, drug design approaches, chemogenomics, synthetic chemistry including combinatorial methods, bioorganic chemistry, natural compounds, high-throughput screening, pharmacological in vitro and in vivo investigations, drug-receptor interactions on the molecular level, structure-activity relationships, drug absorption, distribution, metabolism, elimination, toxicology and pharmacogenomics. In general, special volumes are edited by well known guest editors. , This work is based on the research supported by the South African Research Chairs Initiative of the Department of Science and Technology and National Research Foundation of South Africa (Grant No 98566), the Cancer Association of South Africa (CANSA), Medical Research Council South Africa (MRC-SA) and Rhodes University. The views expressed are those of the authors and should not be attributed to the DST, NRF, CANSA, MRC-SA or Rhodes University. We apologize if we have inadvertently missed any important contributions to the field.
- Full Text: false
- Authors: McAlpine, Shelli R , Edkins, Adrienne L
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66359 , vital:28940 , https://doi.org/10.1007/978-3-319-32607-8
- Description: publisher version , Introduction: Medicinal chemistry is both science and art. The science of medicinal chemistry offers mankind one of its best hopes for improving the quality of life. The art of medicinal chemistry continues to challenge its practitioners with the need for both intuition and experience to discover new drugs. Hence sharing the experience of drug research is uniquely beneficial to the field of medicinal chemistry. Drug research requires interdisciplinary team-work at the interface between chemistry, biology and medicine. Therefore, the topic-related series Topics in Medicinal Chemistry covers all relevant aspects of drug research, e.g. pathobiochemistry of diseases, identification and validation of (emerging) drug targets, structural biology, drugability of targets, drug design approaches, chemogenomics, synthetic chemistry including combinatorial methods, bioorganic chemistry, natural compounds, high-throughput screening, pharmacological in vitro and in vivo investigations, drug-receptor interactions on the molecular level, structure-activity relationships, drug absorption, distribution, metabolism, elimination, toxicology and pharmacogenomics. In general, special volumes are edited by well known guest editors. , This work is based on the research supported by the South African Research Chairs Initiative of the Department of Science and Technology and National Research Foundation of South Africa (Grant No 98566), the Cancer Association of South Africa (CANSA), Medical Research Council South Africa (MRC-SA) and Rhodes University. The views expressed are those of the authors and should not be attributed to the DST, NRF, CANSA, MRC-SA or Rhodes University. We apologize if we have inadvertently missed any important contributions to the field.
- Full Text: false
Herman Charles Bosman: A man of profound contradictions
- Authors: Leff, Carol W
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/458524 , vital:75751 , https://hdl.handle.net/10520/EJC190700
- Description: Herman Charles Bosman is best known for his humorous short stories with signature twist endings. He is less well known for his essays and journalistic writing, which illustrate another side of this enigmatic man. This essay focuses on the paradox that Bosman was (and continues to be) and aims to respond to the following questions: How are we to understand the identity of Herman Charles Bosman? How did he contribute towards an understanding of language and culture in South Africa during his lifetime? Was Bosman consistent in expressing his views through his literary works, or is there evidence of incongruity? These complexities are explored by, first, paying close attention to various biographies on Bosman. His hybrid identity is then illustrated by providing examples of the many pseudonyms he employed over a period of time. This is followed by discussion of several of Bosman's non-fiction pieces, highlighting how contradictions are apparent in his beliefs and philosophy. The essay concludes that Bosman vacillated in both his writing and his beliefs, and was indeed a man of contradictions.
- Full Text:
- Authors: Leff, Carol W
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/458524 , vital:75751 , https://hdl.handle.net/10520/EJC190700
- Description: Herman Charles Bosman is best known for his humorous short stories with signature twist endings. He is less well known for his essays and journalistic writing, which illustrate another side of this enigmatic man. This essay focuses on the paradox that Bosman was (and continues to be) and aims to respond to the following questions: How are we to understand the identity of Herman Charles Bosman? How did he contribute towards an understanding of language and culture in South Africa during his lifetime? Was Bosman consistent in expressing his views through his literary works, or is there evidence of incongruity? These complexities are explored by, first, paying close attention to various biographies on Bosman. His hybrid identity is then illustrated by providing examples of the many pseudonyms he employed over a period of time. This is followed by discussion of several of Bosman's non-fiction pieces, highlighting how contradictions are apparent in his beliefs and philosophy. The essay concludes that Bosman vacillated in both his writing and his beliefs, and was indeed a man of contradictions.
- Full Text:
Heterogeneity in virulence relationships between Cryptophlebia leucotreta granulovirus isolates and geographically distinct host populations: Lessons from codling moth resistance to CpGV-M
- Opoku-Debrah, John K, Hill, Martin P, Knox, Caroline M, Moore, Sean D
- Authors: Opoku-Debrah, John K , Hill, Martin P , Knox, Caroline M , Moore, Sean D
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/417998 , vital:71500 , xlink:href="https://doi.org/10.1007/s10526-016-9728-1"
- Description: In South Africa, the baculovirus Cryptophlebia leucotreta granulovirus (CrleGV) is used to control the citrus pest Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae). However, the risk associated with continuous application of a single active ingredient is resistance. In order to manage resistance should it occur in field populations of T. leucotreta in South Africa, five new CrleGV genotypes from geographically distinct insect populations which were shown to exhibit some degree of phenotypic variation were recovered and genetically characterized. In droplet bioassays using seven CrleGV isolates against five T. leucotreta populations, some isolates were found to show higher virulence to some host populations than others. There were marked differences in the LD50 values of isolates and the number of occlusion bodies required per larva ranged between 0.79 and 3.12. The significance of these findings with respect to the application of CrleGV biopesticides and the management of resistance is discussed.
- Full Text:
- Authors: Opoku-Debrah, John K , Hill, Martin P , Knox, Caroline M , Moore, Sean D
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/417998 , vital:71500 , xlink:href="https://doi.org/10.1007/s10526-016-9728-1"
- Description: In South Africa, the baculovirus Cryptophlebia leucotreta granulovirus (CrleGV) is used to control the citrus pest Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae). However, the risk associated with continuous application of a single active ingredient is resistance. In order to manage resistance should it occur in field populations of T. leucotreta in South Africa, five new CrleGV genotypes from geographically distinct insect populations which were shown to exhibit some degree of phenotypic variation were recovered and genetically characterized. In droplet bioassays using seven CrleGV isolates against five T. leucotreta populations, some isolates were found to show higher virulence to some host populations than others. There were marked differences in the LD50 values of isolates and the number of occlusion bodies required per larva ranged between 0.79 and 3.12. The significance of these findings with respect to the application of CrleGV biopesticides and the management of resistance is discussed.
- Full Text:
Historical perspectives and future directions for access to land, water and related ecosystem services in the Lower Sundays River Valley, South Africa: implications for human well being
- Authors: Chadzingwa, Karabo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/442 , vital:19959
- Description: The legacy of South Africa’s history has facilitated unequal access to land and water resources. In the Lower Sundays River Valley (LSRV), a predominantly commercial farming area, differential access to land and water has impacted aspects of ecosystem service access and human well‐being for disenfranchised families over time. Despite the social, political and economic reform efforts in the past two decades, severe inertia towards efforts attempting to increase equitable access to land and water has been experienced. As a result, communities have mobilized and claimed their land from the government through the land restitution process. Based on a mixed‐methods approach, this research explores the ways in which access to land and water over time has influenced current levels of human well‐being among disenfranchised families. Provisioning and cultural ecosystem services were identified as key areas of loss as a result of forced evictions from land. Freedom of choice was a central and cross‐cutting theme regarding the ability to change levels of human well‐being. Although the loss of ecosystem services associated with land and water had an impact on households, the ecosystem services which are regarded as fundamental to human well‐being do not seem to have been lost. The study recommends the wide use of freedom of choice as an indicator for well‐being in the LSRV, as well as a consideration of subjective, objective and psychological measures of well‐being with regards to natural resources and ecosystem services access. Key agents in fostering desirable pathways toward equity and sustainability in the LSRV are identified with stakeholders as private businesses; inclusive governance; empowered and skilled individuals, as well as NGOs.
- Full Text:
- Authors: Chadzingwa, Karabo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/442 , vital:19959
- Description: The legacy of South Africa’s history has facilitated unequal access to land and water resources. In the Lower Sundays River Valley (LSRV), a predominantly commercial farming area, differential access to land and water has impacted aspects of ecosystem service access and human well‐being for disenfranchised families over time. Despite the social, political and economic reform efforts in the past two decades, severe inertia towards efforts attempting to increase equitable access to land and water has been experienced. As a result, communities have mobilized and claimed their land from the government through the land restitution process. Based on a mixed‐methods approach, this research explores the ways in which access to land and water over time has influenced current levels of human well‐being among disenfranchised families. Provisioning and cultural ecosystem services were identified as key areas of loss as a result of forced evictions from land. Freedom of choice was a central and cross‐cutting theme regarding the ability to change levels of human well‐being. Although the loss of ecosystem services associated with land and water had an impact on households, the ecosystem services which are regarded as fundamental to human well‐being do not seem to have been lost. The study recommends the wide use of freedom of choice as an indicator for well‐being in the LSRV, as well as a consideration of subjective, objective and psychological measures of well‐being with regards to natural resources and ecosystem services access. Key agents in fostering desirable pathways toward equity and sustainability in the LSRV are identified with stakeholders as private businesses; inclusive governance; empowered and skilled individuals, as well as NGOs.
- Full Text:
Hoofletter K Kleurling - 'n besonderse Suid-Afrikaanse
- Authors: Thomas, Cornelius
- Date: 2016
- Language: Afrikaans
- Type: book chapter , text
- Identifier: http://hdl.handle.net/10962/59771 , vital:27647 , http://naledi.online/product/ons-kom-van-ver/
- Description: Hoofletter K Kleurling.
- Full Text: false
- Authors: Thomas, Cornelius
- Date: 2016
- Language: Afrikaans
- Type: book chapter , text
- Identifier: http://hdl.handle.net/10962/59771 , vital:27647 , http://naledi.online/product/ons-kom-van-ver/
- Description: Hoofletter K Kleurling.
- Full Text: false
How content analysis may complement and extend the insights of discourse analysis: An example of research on constructions of abortion in South African newspapers 1978–2005
- Feltham-King, Tracey, Macleod, Catriona I
- Authors: Feltham-King, Tracey , Macleod, Catriona I
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/446258 , vital:74485 , xlink:href="https://doi.org/10.1177/1609406915624575"
- Description: Although discourse analysis is a well-established qualitative research methodology, little attention has been paid to how discourse analysis may be enhanced through careful supplementation with the quantification allowed in content analysis. In this article, we report on a research study that involved the use of both Foucauldian discourse analysis (FDA) and directed content analysis based on social constructionist theory and our qualitative research findings. The research focused on the discourses deployed, and the ways in which women were discursively positioned, in relation to abortion in 300 newspaper articles, published in 25 national and regional South African newspapers over 28 years, from 1978 to 2005. While the FDA was able to illuminate the constitutive network of power relations constructing women as subjects of a particular kind, questions emerged that were beyond the scope of the FDA. These questions concerned understanding the relative weightings of various discourses and tracing historical changes in the deployment of these discourses. In this article, we show how the decision to combine FDA and content analysis affected our sampling methodology. Using specific examples, we illustrate the contribution of the FDA to the study. Then, we indicate how subject positioning formed the link between the FDA and the content analysis. Drawing on the same examples, we demonstrate how the content analysis supplemented the FDA through tracking changes over time and providing empirical evidence of the extent to which subject positionings were deployed.
- Full Text:
- Authors: Feltham-King, Tracey , Macleod, Catriona I
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/446258 , vital:74485 , xlink:href="https://doi.org/10.1177/1609406915624575"
- Description: Although discourse analysis is a well-established qualitative research methodology, little attention has been paid to how discourse analysis may be enhanced through careful supplementation with the quantification allowed in content analysis. In this article, we report on a research study that involved the use of both Foucauldian discourse analysis (FDA) and directed content analysis based on social constructionist theory and our qualitative research findings. The research focused on the discourses deployed, and the ways in which women were discursively positioned, in relation to abortion in 300 newspaper articles, published in 25 national and regional South African newspapers over 28 years, from 1978 to 2005. While the FDA was able to illuminate the constitutive network of power relations constructing women as subjects of a particular kind, questions emerged that were beyond the scope of the FDA. These questions concerned understanding the relative weightings of various discourses and tracing historical changes in the deployment of these discourses. In this article, we show how the decision to combine FDA and content analysis affected our sampling methodology. Using specific examples, we illustrate the contribution of the FDA to the study. Then, we indicate how subject positioning formed the link between the FDA and the content analysis. Drawing on the same examples, we demonstrate how the content analysis supplemented the FDA through tracking changes over time and providing empirical evidence of the extent to which subject positionings were deployed.
- Full Text:
How do editors' attitudes and their perceptions of readers' interests combine with other factors to influence the publication of articles on the natural sciences in the Daily Dispatch?
- Authors: Lang, Steven
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3893 , vital:20553
- Description: This half-thesis examines how editorial values and perceptions determine the quantity and nature of science articles published in the Daily Dispatch, a newspaper distributed through large parts of the Eastern Cape in South Africa. It was predicated on the notion that South African media in general does not cover the natural sciences adequately. In order to test this assumption I decided to investigate the production and publication of science content at the Daily Dispatch as a test case. This study‘s theoretical framework draws on the normative roles of the media in a democracy developed by Christians et al. (2009) and the models of science journalism described by Secko et al. (2012) to demonstrate how two parallel conceptions of democracy set diverse journalistic objectives and engender different types of science content. Having applied an essentially political framework, this thesis uses the Hierarchy of Influences Model devised by Reese and Shoemaker‘s (2014) to explore how an array of forces acting inside and outside the news organisation can shape the publication of science articles. A quantitative content analysis is used to ascertain the number of science articles published in the first six months of 2014. It investigates which science fields received the most coverage, and how prominently the articles are positioned. As the Daily Dispatch does not have any staff dedicated to the science beat, the analysis finds out who produces the science articles that are published. The second phase of this research is a series of interviews with senior editorial staff members aimed at probing the editorial thought processes that determine when and whether specific science stories should be covered. The personal views and biases of the editorial leadership are pivotal to this research because although the newspaper commissioned surveys to determine readership preferences, there were no questions about the sciences. Senior reporters were adamant that they worked for a political newspaper and that as a significant proportion of their readership lived in socio-economically deprived circumstances, they were bound to give priority to articles aimed at improving the lot of their readers. The third phase is a qualitative content analysis of selected articles designed to reveal how science articles are constructed. The final element of this thesis, which ultimately provides an answer to the research question, draws together conclusions from the previous phases to demonstrate the linkage between editorial values and the production of science content.
- Full Text:
- Authors: Lang, Steven
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3893 , vital:20553
- Description: This half-thesis examines how editorial values and perceptions determine the quantity and nature of science articles published in the Daily Dispatch, a newspaper distributed through large parts of the Eastern Cape in South Africa. It was predicated on the notion that South African media in general does not cover the natural sciences adequately. In order to test this assumption I decided to investigate the production and publication of science content at the Daily Dispatch as a test case. This study‘s theoretical framework draws on the normative roles of the media in a democracy developed by Christians et al. (2009) and the models of science journalism described by Secko et al. (2012) to demonstrate how two parallel conceptions of democracy set diverse journalistic objectives and engender different types of science content. Having applied an essentially political framework, this thesis uses the Hierarchy of Influences Model devised by Reese and Shoemaker‘s (2014) to explore how an array of forces acting inside and outside the news organisation can shape the publication of science articles. A quantitative content analysis is used to ascertain the number of science articles published in the first six months of 2014. It investigates which science fields received the most coverage, and how prominently the articles are positioned. As the Daily Dispatch does not have any staff dedicated to the science beat, the analysis finds out who produces the science articles that are published. The second phase of this research is a series of interviews with senior editorial staff members aimed at probing the editorial thought processes that determine when and whether specific science stories should be covered. The personal views and biases of the editorial leadership are pivotal to this research because although the newspaper commissioned surveys to determine readership preferences, there were no questions about the sciences. Senior reporters were adamant that they worked for a political newspaper and that as a significant proportion of their readership lived in socio-economically deprived circumstances, they were bound to give priority to articles aimed at improving the lot of their readers. The third phase is a qualitative content analysis of selected articles designed to reveal how science articles are constructed. The final element of this thesis, which ultimately provides an answer to the research question, draws together conclusions from the previous phases to demonstrate the linkage between editorial values and the production of science content.
- Full Text:
How do social and personal identity, sense of place, connectedness to nature and environmental understanding influence the implementation of collective, large-scale biodiversity stewardship initiatives in South Africa?
- Authors: Potts, Tracey Ann
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3663 , vital:20534
- Description: Biodiversity stewardship is a mechanism that is used to conserve high value biodiversity assets. Biodiversity stewardship programmes focus on areas that are under immediate threat from development or under medium to long term threat from degradation or transformation that will result in habitat loss. Biodiversity stewardship provides a solution to the resource crisis being faced by many state and provincial conservation agencies, NGO’s (Non-Governmental Organisations) and PBO’s (Public Benefit Organisations) in that it facilitates the declaration, and the subsequent improved conservation management, of private or communally owned land whilst still retaining the existing tenure. The rate of habitat loss can often be slowed, or even reversed, by proactively securing these areas and facilitating management decision-making with a focus on biodiversity outcomes without the capital investment required by the State to purchase the land. In return for conservation management actions, certain land-use restrictions and the associated opportunity costs, the State offers a suite of incentives and benefits that are, where possible, tailored to meet the needs of the landowner. Particular regions of South Africa lend themselves well to the development of biodiversity stewardship initiatives which are designed to secure ecological processes and ecosystems across a landscape or an ecological feature at scales of tens of thousands of hectares. When developing landscape level biodiversity stewardship initiatives, negotiations tend to be focussed on groups of landowners. This requires collaboration and the collective alignment of natural resource management decision-making and conservation actions amongst neighbours. Gaining a better understanding of how the social constructs of ecological understanding, place attachment, connectedness to nature, occupational identity and social and personal identity influence decision-making, behaviour and group structure is a critically important factor when developing a tool to predict the likelihood of landowners to collectively commit to long-term, legally binding biodiversity stewardship programmes. The overarching hypothesis was that the social constructs listed above influence group dynamics within the context of collective pro-conservation behaviour. Social dynamics associated with large-scale biodiversity stewardship initiatives become complex when multiple landowners are involved. Could social cohesion and group culture be influenced by aspects of identity and do these in turn develop into barriers or motivators to coordinated and sustained conservation efforts? Further influences on the successful implementation of landscape scale biodiversity stewardship initiatives could include ecological understanding, connectedness to nature and place attachment. Structured interviews were held with the landowners engaged in two separate large-scale biodiversity stewardship sites, the Compassberg Protected Environment and the Baviaanskloof Hartland, in the Eastern Cape Province of South Africa. The interviews were followed up with surveys containing psychometric scales related to the influence of ecological understanding, place attachment, connectedness to nature and aspects of identity on commitment to collective long-term, large-scale biodiversity stewardship initiatives. The results from a set of non-parametric (exact) Wilcoxon rank-sum tests showed that scores on the new ecological paradigm scale and the place attachment scale latent variables were significantly different at the two study sites, at the 10% level of significance. Demographic differences between the two study sites influenced group dynamics, collective decision-making and commitment. The relationship between the latent variables (the five psychometric scales measured) and the ancillary variables (the demographic data describing the respondents) cannot be considered conclusive; however they do provide relatively useful insights into the development of a scale or tool to measure conservation opportunity. The thesis concludes with a proposed conservation opportunity assessment tool that can be utilised alongside the existing, well refined, conservation priority assessment tools to assist in decision-making when planning large, landscape scale biodiversity stewardship initiatives in South Africa.
- Full Text:
- Authors: Potts, Tracey Ann
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3663 , vital:20534
- Description: Biodiversity stewardship is a mechanism that is used to conserve high value biodiversity assets. Biodiversity stewardship programmes focus on areas that are under immediate threat from development or under medium to long term threat from degradation or transformation that will result in habitat loss. Biodiversity stewardship provides a solution to the resource crisis being faced by many state and provincial conservation agencies, NGO’s (Non-Governmental Organisations) and PBO’s (Public Benefit Organisations) in that it facilitates the declaration, and the subsequent improved conservation management, of private or communally owned land whilst still retaining the existing tenure. The rate of habitat loss can often be slowed, or even reversed, by proactively securing these areas and facilitating management decision-making with a focus on biodiversity outcomes without the capital investment required by the State to purchase the land. In return for conservation management actions, certain land-use restrictions and the associated opportunity costs, the State offers a suite of incentives and benefits that are, where possible, tailored to meet the needs of the landowner. Particular regions of South Africa lend themselves well to the development of biodiversity stewardship initiatives which are designed to secure ecological processes and ecosystems across a landscape or an ecological feature at scales of tens of thousands of hectares. When developing landscape level biodiversity stewardship initiatives, negotiations tend to be focussed on groups of landowners. This requires collaboration and the collective alignment of natural resource management decision-making and conservation actions amongst neighbours. Gaining a better understanding of how the social constructs of ecological understanding, place attachment, connectedness to nature, occupational identity and social and personal identity influence decision-making, behaviour and group structure is a critically important factor when developing a tool to predict the likelihood of landowners to collectively commit to long-term, legally binding biodiversity stewardship programmes. The overarching hypothesis was that the social constructs listed above influence group dynamics within the context of collective pro-conservation behaviour. Social dynamics associated with large-scale biodiversity stewardship initiatives become complex when multiple landowners are involved. Could social cohesion and group culture be influenced by aspects of identity and do these in turn develop into barriers or motivators to coordinated and sustained conservation efforts? Further influences on the successful implementation of landscape scale biodiversity stewardship initiatives could include ecological understanding, connectedness to nature and place attachment. Structured interviews were held with the landowners engaged in two separate large-scale biodiversity stewardship sites, the Compassberg Protected Environment and the Baviaanskloof Hartland, in the Eastern Cape Province of South Africa. The interviews were followed up with surveys containing psychometric scales related to the influence of ecological understanding, place attachment, connectedness to nature and aspects of identity on commitment to collective long-term, large-scale biodiversity stewardship initiatives. The results from a set of non-parametric (exact) Wilcoxon rank-sum tests showed that scores on the new ecological paradigm scale and the place attachment scale latent variables were significantly different at the two study sites, at the 10% level of significance. Demographic differences between the two study sites influenced group dynamics, collective decision-making and commitment. The relationship between the latent variables (the five psychometric scales measured) and the ancillary variables (the demographic data describing the respondents) cannot be considered conclusive; however they do provide relatively useful insights into the development of a scale or tool to measure conservation opportunity. The thesis concludes with a proposed conservation opportunity assessment tool that can be utilised alongside the existing, well refined, conservation priority assessment tools to assist in decision-making when planning large, landscape scale biodiversity stewardship initiatives in South Africa.
- Full Text:
Hsp40 Co-chaperones as drug targets: towards the development of specific inhibitors
- Pesce, Eva-Rachele, Blatch, Gregory L, Edkins, Adrienne L
- Authors: Pesce, Eva-Rachele , Blatch, Gregory L , Edkins, Adrienne L
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66335 , vital:28937 , https://doi.org/10.1007/7355_2015_92
- Description: publisher version , The heat shock protein 40 (Hsp40/DNAJ) family of co-chaperones modulates the activity of the major molecular chaperone heat shock protein 70 (Hsp70) protein group. Hsp40 stimulates the basal ATPase activity of Hsp70 and hence regulates the affinity of Hsp70 for substrate proteins. The number of Hsp40 genes in most organisms is substantially greater than the number of Hsp70 genes. Therefore, different Hsp40 family members may regulate different activities of the same Hsp70. This fact, along with increasing knowledge of the function of Hsp40 in diseases, has led to certain Hsp40 isoforms being considered promising drug targets. Here we review the role of Hsp40 in human disease and recent developments towards the creation of Hsp40-specific inhibitors.
- Full Text: false
- Authors: Pesce, Eva-Rachele , Blatch, Gregory L , Edkins, Adrienne L
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66335 , vital:28937 , https://doi.org/10.1007/7355_2015_92
- Description: publisher version , The heat shock protein 40 (Hsp40/DNAJ) family of co-chaperones modulates the activity of the major molecular chaperone heat shock protein 70 (Hsp70) protein group. Hsp40 stimulates the basal ATPase activity of Hsp70 and hence regulates the affinity of Hsp70 for substrate proteins. The number of Hsp40 genes in most organisms is substantially greater than the number of Hsp70 genes. Therefore, different Hsp40 family members may regulate different activities of the same Hsp70. This fact, along with increasing knowledge of the function of Hsp40 in diseases, has led to certain Hsp40 isoforms being considered promising drug targets. Here we review the role of Hsp40 in human disease and recent developments towards the creation of Hsp40-specific inhibitors.
- Full Text: false
Hsp90 co-chaperones as drug targets in cancer: current perspectives
- Authors: Edkins, Adrienne L
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66347 , vital:28938 , https://doi.org/10.1007/7355_2015_99
- Description: publisher version , Hsp90 is a molecular chaperone that regulates the function of numerous oncogenic transcription factors and signalling intermediates in the cell. Inhibition of Hsp90 is sufficient to induce the proteosomal degradation of many of these proteins, and as such, the Hsp90 chaperone has been regarded as a promising drug target. The appropriate functioning of the Hsp90 chaperone is dependent on its ATPase activity and interactions with a cohort of non-substrate accessory proteins known as co-chaperones. Co-chaperones associate with Hsp90 at all stages of the chaperone cycle and regulate a range of Hsp90 functions, including ATP hydrolysis and client protein binding and release. Given the ability of co-chaperones to organise the function of the Hsp90 molecular machine, these proteins are now regarded as potential drug targets. Herein the role of selected Hsp90 co-chaperones Hop, Cdc37, p23 and Aha1 as possible drug targets is discussed with a focus on cancer. , This work is based on the research supported by the South African Research Chairs Initiative of the Department of Science and Technology and National Research Foundation of South Africa (Grant No 98566), the Cancer Association of South Africa (CANSA), Medical Research Council South Africa (MRC-SA) and Rhodes University. The views expressed are those of the authors and should not be attributed to the DST, NRF, CANSA, MRC-SA or Rhodes University. We apologize if we have inadvertently missed any important contributions to the field.
- Full Text: false
- Authors: Edkins, Adrienne L
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66347 , vital:28938 , https://doi.org/10.1007/7355_2015_99
- Description: publisher version , Hsp90 is a molecular chaperone that regulates the function of numerous oncogenic transcription factors and signalling intermediates in the cell. Inhibition of Hsp90 is sufficient to induce the proteosomal degradation of many of these proteins, and as such, the Hsp90 chaperone has been regarded as a promising drug target. The appropriate functioning of the Hsp90 chaperone is dependent on its ATPase activity and interactions with a cohort of non-substrate accessory proteins known as co-chaperones. Co-chaperones associate with Hsp90 at all stages of the chaperone cycle and regulate a range of Hsp90 functions, including ATP hydrolysis and client protein binding and release. Given the ability of co-chaperones to organise the function of the Hsp90 molecular machine, these proteins are now regarded as potential drug targets. Herein the role of selected Hsp90 co-chaperones Hop, Cdc37, p23 and Aha1 as possible drug targets is discussed with a focus on cancer. , This work is based on the research supported by the South African Research Chairs Initiative of the Department of Science and Technology and National Research Foundation of South Africa (Grant No 98566), the Cancer Association of South Africa (CANSA), Medical Research Council South Africa (MRC-SA) and Rhodes University. The views expressed are those of the authors and should not be attributed to the DST, NRF, CANSA, MRC-SA or Rhodes University. We apologize if we have inadvertently missed any important contributions to the field.
- Full Text: false
Hungry on arrival
- Authors: Mofokeng, Kabelo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6020 , http://hdl.handle.net/10962/d1021244
- Description: My collection embraces different kinds of poetry, I also write my own journey through my home in Pimville Soweto, the urban sounds and multilingual speech patterns as I move through it. Other poems draw on the Sesotho culture and tradition which still runs strongly in my family, and here I integrate the different kinds of sounds and associations of Sesotho and English in the same poem. I am inspired by the works and lives of artists and poets such as, Mafika Gwala, Jackson Hlungwani, and Herbie Tsoaeli amongst others. Other poems emerging are dense and self-reflective manifesting a transitional period whereby the poet experiments with form and style in the manner of Arthur Nortje and Angifi Dladla.
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- Authors: Mofokeng, Kabelo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6020 , http://hdl.handle.net/10962/d1021244
- Description: My collection embraces different kinds of poetry, I also write my own journey through my home in Pimville Soweto, the urban sounds and multilingual speech patterns as I move through it. Other poems draw on the Sesotho culture and tradition which still runs strongly in my family, and here I integrate the different kinds of sounds and associations of Sesotho and English in the same poem. I am inspired by the works and lives of artists and poets such as, Mafika Gwala, Jackson Hlungwani, and Herbie Tsoaeli amongst others. Other poems emerging are dense and self-reflective manifesting a transitional period whereby the poet experiments with form and style in the manner of Arthur Nortje and Angifi Dladla.
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I have gone away many times
- Metileni, Moses Nzama Khaizen
- Authors: Metileni, Moses Nzama Khaizen
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6010 , http://hdl.handle.net/10962/d1021225
- Description: I search for beauty and for myself amidst the debris and ruin and violence, so my poems are mainly grounded narratives that combine the lyrical and the political, celebration and lament. They reconnect with nature, the now ravaged landscapes that gave me my first impulse to make songs, poetry, and art. My poems also draw on my Xitsonga culture – its folklore, proverbs, idioms, parables, and clan praise songs. Stylistically I am influenced by Mahmoud Darwish’s and Garcia Lorca’s musical structures, while Aimé Césaire has shown me how to write a long poem in both abstract and concrete registers, and Yehuda Amichai how to write with a questioning style, dislodging accepted dogma. Local influences are Mzi Mahola and James Magaisa, with their critique and celebration of culture. I pick up fragments elsewhere, as I go.
- Full Text:
- Authors: Metileni, Moses Nzama Khaizen
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6010 , http://hdl.handle.net/10962/d1021225
- Description: I search for beauty and for myself amidst the debris and ruin and violence, so my poems are mainly grounded narratives that combine the lyrical and the political, celebration and lament. They reconnect with nature, the now ravaged landscapes that gave me my first impulse to make songs, poetry, and art. My poems also draw on my Xitsonga culture – its folklore, proverbs, idioms, parables, and clan praise songs. Stylistically I am influenced by Mahmoud Darwish’s and Garcia Lorca’s musical structures, while Aimé Césaire has shown me how to write a long poem in both abstract and concrete registers, and Yehuda Amichai how to write with a questioning style, dislodging accepted dogma. Local influences are Mzi Mahola and James Magaisa, with their critique and celebration of culture. I pick up fragments elsewhere, as I go.
- Full Text: