Knowledge, Attitudes and Perceptions Towards HIV Testing Among IsiXhosa-Speaking Men in The Zithulele Catchment Area of The Rural Eastern Cape Province, South Africa
- Authors: Scotcher, Philippa Claire
- Date: 2021-10
- Subjects: HIV infections
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/6842 , vital:51017
- Description: BACKGROUND South Africa carries the largest burden of Human Immunodeficiency Virus (HIV) in the world, with over 7.9 million people infected and over 70 000 HIV-related deaths in 2019. Men are 25% more likely to die from Acquired Immune Deficiency Syndrome (AIDS) compared to women, even though women are more likely to be infected. Despite these numbers, only 24.1% of HIV-positive men compared to 64.8% of HIV-positive women were aware of their status. Statistics indicate that men in South Africa are not testing for HIV until it is too late. In order to focus efforts on the prevention of HIV transmission, there is a need to understand why men are not accessing HIV testing and treatment services earlier. This study aims to explore the knowledge, attitudes, and perceptions towards HIV testing of isiXhosa-speaking men in the Zithulele catchment area of the rural Eastern Cape. METHODS This was a qualitative study using the phenomenological approach. It was conducted among a purposive sample of isiXhosa-speaking men from the Zithulele catchment area, in the OR Tambo district of the Eastern Cape Province. Ten semi-structured interviews and one focus group were conducted in isiXhosa. Interviews were audiorecorded, transcribed, and translated into English. The interviews were thematically analysed using an inductive approach. RESULTS Participants from the study had a good understanding of HIV and HIV transmission. They perceived HIV infection as a death sentence, a consequence of immoral behaviour and an indication of failure as man. Reluctance to test for HIV was due to the perception that testing hastens the onset of symptoms and death, whereas disclosure of an HIV-positive status was described as difficult due to the fear of stigmatization. Some of the barriers to accessing HIV testing services included masculine norms, the belief that sickness is equated with weakness, a perceived lack of confidentiality at health facilities and how female-dominated clinics were not male-friendly spaces. Suggestions to improve HIV testing among men included improving targeted education, home-based testing services and utilizing traditional meetings to address men. CONCLUSION The findings of this study may suggest that healthy men in the Zithulele catchment area of the rural Eastern Cape are not accessing HIV testing and treatment services. The reasons behind this reluctance include false beliefs around HIV and testing, the fear of discrimination, disruption of masculine norms and reluctance to access care at female-dominated health facilities. Further research is needed to explore ways to reach, educate and encourage men to test earlier for HIV. , Thesis (Masters) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-10
- Authors: Scotcher, Philippa Claire
- Date: 2021-10
- Subjects: HIV infections
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/6842 , vital:51017
- Description: BACKGROUND South Africa carries the largest burden of Human Immunodeficiency Virus (HIV) in the world, with over 7.9 million people infected and over 70 000 HIV-related deaths in 2019. Men are 25% more likely to die from Acquired Immune Deficiency Syndrome (AIDS) compared to women, even though women are more likely to be infected. Despite these numbers, only 24.1% of HIV-positive men compared to 64.8% of HIV-positive women were aware of their status. Statistics indicate that men in South Africa are not testing for HIV until it is too late. In order to focus efforts on the prevention of HIV transmission, there is a need to understand why men are not accessing HIV testing and treatment services earlier. This study aims to explore the knowledge, attitudes, and perceptions towards HIV testing of isiXhosa-speaking men in the Zithulele catchment area of the rural Eastern Cape. METHODS This was a qualitative study using the phenomenological approach. It was conducted among a purposive sample of isiXhosa-speaking men from the Zithulele catchment area, in the OR Tambo district of the Eastern Cape Province. Ten semi-structured interviews and one focus group were conducted in isiXhosa. Interviews were audiorecorded, transcribed, and translated into English. The interviews were thematically analysed using an inductive approach. RESULTS Participants from the study had a good understanding of HIV and HIV transmission. They perceived HIV infection as a death sentence, a consequence of immoral behaviour and an indication of failure as man. Reluctance to test for HIV was due to the perception that testing hastens the onset of symptoms and death, whereas disclosure of an HIV-positive status was described as difficult due to the fear of stigmatization. Some of the barriers to accessing HIV testing services included masculine norms, the belief that sickness is equated with weakness, a perceived lack of confidentiality at health facilities and how female-dominated clinics were not male-friendly spaces. Suggestions to improve HIV testing among men included improving targeted education, home-based testing services and utilizing traditional meetings to address men. CONCLUSION The findings of this study may suggest that healthy men in the Zithulele catchment area of the rural Eastern Cape are not accessing HIV testing and treatment services. The reasons behind this reluctance include false beliefs around HIV and testing, the fear of discrimination, disruption of masculine norms and reluctance to access care at female-dominated health facilities. Further research is needed to explore ways to reach, educate and encourage men to test earlier for HIV. , Thesis (Masters) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-10
Adolescent sexual reproductive health and rights in the Alice area, Eastern Cape Province of South Africa
- Authors: Moko, Zukhanye
- Date: 2021-02
- Subjects: Reproductive health , Right to health , HIV infections
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20676 , vital:46423
- Description: Sexual Reproductive Health (SRH) is a significant aspect of adolescent’s growth. Adolescents particularly girls face the risk of exposure to Human Immunodeficiency Virus (HIV), Sexually Transmitted Diseases (STDs), child marriages, high rates of unwanted pregnancy and the risk of those pregnancies can lead to unsafe abortion. In South Africa, considerable progress has been made in achieving improved access to Sexual Reproductive Health and Rights (SRHR) among the general population, however, some factors influencing SRHR of adolescents and young people have been slow to achieve. The study aims to investigate factors influencing Sexual Reproductive Health and Rights of adolescents in Alice, which is located in the Eastern Cape Province of South Africa. The Social-Ecological Model was considered appropriate for this study as it provides a comprehensive understanding of the multiple and interacting determinants of Sexual Reproductive Health and Rights. A qualitative methodology was adopted, involving focus groups with high school learners, in-depth interviews with institutional actors (Department of Health, Basic Education and Social Development), and participant observations. The study reveals that adolescents’ have access to Sexual Reproductive Health services from healthcare centres but only a few utilize or access them due to barriers such as the geographical location, denial and judgement about young people's sexuality limits their access to comprehensive knowledge to protect and promote their Sexual and Reproductive Health. The findings show that the adolescents who were most affected by Sexual Reproductive Health and Rights challenges were those from deep rural areas. They had minimal information/education compared to those residing in areas close to the town of Alice and major roads. Multi-sectoral interventions empowering adolescents and young people to exercise their rights to optimize SRHR service yield better results. , Thesis (MSc) -- Faculty of Science & Agriculture, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Moko, Zukhanye
- Date: 2021-02
- Subjects: Reproductive health , Right to health , HIV infections
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20676 , vital:46423
- Description: Sexual Reproductive Health (SRH) is a significant aspect of adolescent’s growth. Adolescents particularly girls face the risk of exposure to Human Immunodeficiency Virus (HIV), Sexually Transmitted Diseases (STDs), child marriages, high rates of unwanted pregnancy and the risk of those pregnancies can lead to unsafe abortion. In South Africa, considerable progress has been made in achieving improved access to Sexual Reproductive Health and Rights (SRHR) among the general population, however, some factors influencing SRHR of adolescents and young people have been slow to achieve. The study aims to investigate factors influencing Sexual Reproductive Health and Rights of adolescents in Alice, which is located in the Eastern Cape Province of South Africa. The Social-Ecological Model was considered appropriate for this study as it provides a comprehensive understanding of the multiple and interacting determinants of Sexual Reproductive Health and Rights. A qualitative methodology was adopted, involving focus groups with high school learners, in-depth interviews with institutional actors (Department of Health, Basic Education and Social Development), and participant observations. The study reveals that adolescents’ have access to Sexual Reproductive Health services from healthcare centres but only a few utilize or access them due to barriers such as the geographical location, denial and judgement about young people's sexuality limits their access to comprehensive knowledge to protect and promote their Sexual and Reproductive Health. The findings show that the adolescents who were most affected by Sexual Reproductive Health and Rights challenges were those from deep rural areas. They had minimal information/education compared to those residing in areas close to the town of Alice and major roads. Multi-sectoral interventions empowering adolescents and young people to exercise their rights to optimize SRHR service yield better results. , Thesis (MSc) -- Faculty of Science & Agriculture, 2021
- Full Text:
- Date Issued: 2021-02
Genetic characteristics of Human Immunodeficiency Virus-1, and deter-minants of late presentation for care and Diabetes mellitus amongst newly diagnosed Human Immunodeficiency Virus positive patients in the Eastern Cape, South Africa.
- Authors: Sogbanmu, Olufunso Oladipo
- Date: 2019
- Subjects: HIV infections
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/${Handle} , vital:40495
- Description: Phylogenies may help to characterize transmission pairs, enhance contact tracing and outbreak investigations, track the origin and spread of epidemics over place and time, and to identify patterns of onward Human Immunodeficiency-Virus (HIV) transmission among risk groups. If the pattern and evolution of HIV drug resistance can be mapped, this may influence the development of guidelines in the clinical management of HIV especially with issues relating to prevalence of primary drug resistance and its impact on outcomes of present antiretroviral treatment (ART) regimen in use and the ability to trace and track the development of drug resistant strains. The roll-out of the test and treat Programme for newly diagnosed HIV infected pa-tient, seeks to identify HIV infected individuals early and to prevent morbidity and mortality associated with the late presentation for HIV care. The determination of the magnitude of ‘late presentation’ and or ‘presentation with advanced HIV disease’ can be used in very diverse settings and for many purposes. It provides a unified way to define the problem, thereby targeting appropriate interventions to prevent the detrimental outcomes associated with late presentation to care. The subtle relationship between HIV and diabetes mellitus (DM) may also help in formulating better preventive programs to aid the control of non-communicable diseases such as DM. This cross-sectional study includes a purposive selection of 335 HIV positive patients attending the voluntary counselling and Testing (VCT) and HIV Counselling and Testing (HCT) centres and outpatient departments at Cecilia Makiwane Hospital and the HCT sites at the Buffalo District municipality community health centres, Eastern Cape Province, South Africa. Late or delayed presentation of HIV infection was defined as CD4 cell count beneath 350 cells/μL and/or patients presenting with an AIDS-defining event at the first follow-up regardless of the CD4 cell count. Chapter 1 provides the general introduction had an overview of the introduction to the study, the statement of the research problem, hypothesis, the aim and the objectives. xx Chapter 2 looked in-depth at the HIV, case definition, the latest epidemiology of HIV, the HIV genome, the life cycle of HIV, its diagnosis, the classes of antiretroviral drugs, development of drug resistance. Chapter 3 highlighted the prevalence of Transmitted Drug Resistance (TDR) with focus on the protease gene. RNA was extracted from blood samples of 72 newly diagnosed HIV-1 patients attending some HIV testing and counselling clinics from August 2016 to July 2017. Protease fragments were amplified with specific primers by RT-PCR followed by nested PCR. The amplified products were sequenced using the ABI 360 sequencer, edited with Geneious version 9.1.5 and translated into amino acid with BioEdit software. Drug related resistance mutation (DRMs) analysis was performed on all the protease sequences in accordance with the 2009 WHO list of surveillance drug resistance mutations by submitting the edited sequences to Stanford HIV drug interpretation programme and the international AIDS society-USA guidelines for query of drug resistance associated mutations while phylogenetic analysis was performed using MEGA 6 to allocate all viral sequences into subtypes. In the study, a total of 52/72 (71.1%) reliable HIV-1 protease sequences were obtained in which subtyping and drug resistance mutations were performed. Two (3.8%) major Protease resistance associated mutation (V82A/L and L90M) were observed while another polymorphism like L10F, T74S, Q58E, L10I/V and M46V were also identified. Phylogenetic analyses delineated all the sequences as HIV-1 subtype C. Chapter 4 describes the prevalence and the determinants of late presentation amongst newly diagnosed HIV positive individuals in the Eastern Cape. It indicates the extent of the prevalence of patients presenting to care and at what HIV stage they were assessing health care services since the inception of the ‘test and treat’ strategy. It is a cross-sectional study where a total of 335 newly diagnosed patients were recruited consecutively be-tween August 2016 and July 2017. Late presenter for HIV care was defined in accordance with the European Late Presenter Consensus working group as a patient who reports for care when the CD4 count is below 350 cells/μL and/or when there is an established Aids- xxi defining clinical condition, irrespective of CD4 count. Adjusted and unadjusted logistic regression analysis was used to examine the determinants of late HIV diagnosis. The study showed that 60% of patients were late presenters, with 35% presenting with advanced disease. The major determinants identified were being male and low level of education. This led to recommendations directed at ensuring programmes that targets men in identifying their HIV status and assess care at early stage to prevents the morbidity and mortality associated with delayed presentation. Also, it was recommended that effort should be made to improve access to education and also include HIV related topics into the educational curriculum. Chapter 5 aimed to describe the prevalence and determinants of DM amongst newly diagnosed HIV positive individuals. This is a cross-sectional study which recruited 335 patients between August 2016 and September 2017. Definition for diabetes mellitus was made based on the SEDMSA 2015 guideline of HBA1C of above 6.5%. Adjusted and unadjusted logistic regression analysis was used to examine the determinants of abnormal glycated haemoglobin. Findings showed the prevalence of DM at about 6% amongst newly diagnosed HIV positive individuals. This is similar to findings in other study within the country, but a bit lower than what was obtained in the developed countries. The role of older age (above 40 years) as predisposing factor to development of diabetes in newly diagnosed HIV positive individual was well noted and taken. This ensures that screening for DM should be targeted at elderly HIV positive individuals. The grey area of the appropriate mode of diagnostic test to use to diagnose HIV is still debatable, however, a combination of HBA1c and fasting blood sugar (FBS) may improve the diagnosis of DM in this population group. In chapter 6, the general conclusions, recommendations and future perspectives of the study are reflected.
- Full Text:
- Date Issued: 2019
- Authors: Sogbanmu, Olufunso Oladipo
- Date: 2019
- Subjects: HIV infections
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/${Handle} , vital:40495
- Description: Phylogenies may help to characterize transmission pairs, enhance contact tracing and outbreak investigations, track the origin and spread of epidemics over place and time, and to identify patterns of onward Human Immunodeficiency-Virus (HIV) transmission among risk groups. If the pattern and evolution of HIV drug resistance can be mapped, this may influence the development of guidelines in the clinical management of HIV especially with issues relating to prevalence of primary drug resistance and its impact on outcomes of present antiretroviral treatment (ART) regimen in use and the ability to trace and track the development of drug resistant strains. The roll-out of the test and treat Programme for newly diagnosed HIV infected pa-tient, seeks to identify HIV infected individuals early and to prevent morbidity and mortality associated with the late presentation for HIV care. The determination of the magnitude of ‘late presentation’ and or ‘presentation with advanced HIV disease’ can be used in very diverse settings and for many purposes. It provides a unified way to define the problem, thereby targeting appropriate interventions to prevent the detrimental outcomes associated with late presentation to care. The subtle relationship between HIV and diabetes mellitus (DM) may also help in formulating better preventive programs to aid the control of non-communicable diseases such as DM. This cross-sectional study includes a purposive selection of 335 HIV positive patients attending the voluntary counselling and Testing (VCT) and HIV Counselling and Testing (HCT) centres and outpatient departments at Cecilia Makiwane Hospital and the HCT sites at the Buffalo District municipality community health centres, Eastern Cape Province, South Africa. Late or delayed presentation of HIV infection was defined as CD4 cell count beneath 350 cells/μL and/or patients presenting with an AIDS-defining event at the first follow-up regardless of the CD4 cell count. Chapter 1 provides the general introduction had an overview of the introduction to the study, the statement of the research problem, hypothesis, the aim and the objectives. xx Chapter 2 looked in-depth at the HIV, case definition, the latest epidemiology of HIV, the HIV genome, the life cycle of HIV, its diagnosis, the classes of antiretroviral drugs, development of drug resistance. Chapter 3 highlighted the prevalence of Transmitted Drug Resistance (TDR) with focus on the protease gene. RNA was extracted from blood samples of 72 newly diagnosed HIV-1 patients attending some HIV testing and counselling clinics from August 2016 to July 2017. Protease fragments were amplified with specific primers by RT-PCR followed by nested PCR. The amplified products were sequenced using the ABI 360 sequencer, edited with Geneious version 9.1.5 and translated into amino acid with BioEdit software. Drug related resistance mutation (DRMs) analysis was performed on all the protease sequences in accordance with the 2009 WHO list of surveillance drug resistance mutations by submitting the edited sequences to Stanford HIV drug interpretation programme and the international AIDS society-USA guidelines for query of drug resistance associated mutations while phylogenetic analysis was performed using MEGA 6 to allocate all viral sequences into subtypes. In the study, a total of 52/72 (71.1%) reliable HIV-1 protease sequences were obtained in which subtyping and drug resistance mutations were performed. Two (3.8%) major Protease resistance associated mutation (V82A/L and L90M) were observed while another polymorphism like L10F, T74S, Q58E, L10I/V and M46V were also identified. Phylogenetic analyses delineated all the sequences as HIV-1 subtype C. Chapter 4 describes the prevalence and the determinants of late presentation amongst newly diagnosed HIV positive individuals in the Eastern Cape. It indicates the extent of the prevalence of patients presenting to care and at what HIV stage they were assessing health care services since the inception of the ‘test and treat’ strategy. It is a cross-sectional study where a total of 335 newly diagnosed patients were recruited consecutively be-tween August 2016 and July 2017. Late presenter for HIV care was defined in accordance with the European Late Presenter Consensus working group as a patient who reports for care when the CD4 count is below 350 cells/μL and/or when there is an established Aids- xxi defining clinical condition, irrespective of CD4 count. Adjusted and unadjusted logistic regression analysis was used to examine the determinants of late HIV diagnosis. The study showed that 60% of patients were late presenters, with 35% presenting with advanced disease. The major determinants identified were being male and low level of education. This led to recommendations directed at ensuring programmes that targets men in identifying their HIV status and assess care at early stage to prevents the morbidity and mortality associated with delayed presentation. Also, it was recommended that effort should be made to improve access to education and also include HIV related topics into the educational curriculum. Chapter 5 aimed to describe the prevalence and determinants of DM amongst newly diagnosed HIV positive individuals. This is a cross-sectional study which recruited 335 patients between August 2016 and September 2017. Definition for diabetes mellitus was made based on the SEDMSA 2015 guideline of HBA1C of above 6.5%. Adjusted and unadjusted logistic regression analysis was used to examine the determinants of abnormal glycated haemoglobin. Findings showed the prevalence of DM at about 6% amongst newly diagnosed HIV positive individuals. This is similar to findings in other study within the country, but a bit lower than what was obtained in the developed countries. The role of older age (above 40 years) as predisposing factor to development of diabetes in newly diagnosed HIV positive individual was well noted and taken. This ensures that screening for DM should be targeted at elderly HIV positive individuals. The grey area of the appropriate mode of diagnostic test to use to diagnose HIV is still debatable, however, a combination of HBA1c and fasting blood sugar (FBS) may improve the diagnosis of DM in this population group. In chapter 6, the general conclusions, recommendations and future perspectives of the study are reflected.
- Full Text:
- Date Issued: 2019
Psychosocial challenges faced by people living with hiv/aids at Victoria Hospital, Alice, Nkonkobe Municipality, Eastern Cape
- Authors: Sam, Ntombifikile
- Date: 2019
- Subjects: HIV infections
- Language: English
- Type: Thesis , Masters , MSW
- Identifier: http://hdl.handle.net/10353/16210 , vital:40698
- Description: This study investigated the psychosocial challenges faced by people living with HIV/AIDS at Victoria Hospital, Alice, Nkonkobe municipality in the Eastern Cape. This study explored the views, attitudes and perceptions of people living with HIV/AIDS to understand the psychosocial challenges they underwent. The study aimed at achieving the following specific objectives: (i) To investigate the psychosocial challenges faced by people living with HIV and AIDS at the Victoria Hospital. (ii) To assess the impact of families and communities to people living with HIV/AIDS with respect to the lack of psychosocial support provided to people who are infected with HIV and AIDS at the Victoria Hospital in the Alice Area of the province of the Eastern Cape. (iii) To identify possible psychosocial support systems that could be provided by social service practitioners and by social networks for people living with HIV and AIDS and their families. The study used qualitative research approach where in-depth interviews and a focus group with key informants were used as methods of data collection. The study was premised on the systems theory to give a theoretical orientation into understanding the psychosocial challenges of people living with HIV/AIDS and possible support to be given to them. The sample size was made up of 10 patient participants; eight (8) ii females, two (2) males; and three (3) health practitioners. Non-probability sampling technique was used in the study. Specifically, purposive sampling was used to select the participants because the researcher believed that the participants had knowledge and experiences about HIV/AIDS. The data was analysed qualitatively through a content thematic analysis technique. The study found that there are many psychosocial challenges that are faced by people living with HIV/AIDS such as poverty, social support, rejection, depression and stigma and discrimination. The study recommends that the affected communities should have support groups for people living with HIV/AIDS to empower them and their families. The study also recommends the implementation of community engagements in the form of educating communities about HIV/AIDS and the importance of supporting people living with HIV/AIDS. More emphasis on the right to equality and the right for everyone to be treated with dignity and respect, as stated by Chapter 2 of the Bill of Rights of the South African constitution is highly recommended. Based on the evidence provided through the findings, the researcher is optimistic that all the challenges presented will be taken into consideration in future plans and policies.
- Full Text:
- Date Issued: 2019
- Authors: Sam, Ntombifikile
- Date: 2019
- Subjects: HIV infections
- Language: English
- Type: Thesis , Masters , MSW
- Identifier: http://hdl.handle.net/10353/16210 , vital:40698
- Description: This study investigated the psychosocial challenges faced by people living with HIV/AIDS at Victoria Hospital, Alice, Nkonkobe municipality in the Eastern Cape. This study explored the views, attitudes and perceptions of people living with HIV/AIDS to understand the psychosocial challenges they underwent. The study aimed at achieving the following specific objectives: (i) To investigate the psychosocial challenges faced by people living with HIV and AIDS at the Victoria Hospital. (ii) To assess the impact of families and communities to people living with HIV/AIDS with respect to the lack of psychosocial support provided to people who are infected with HIV and AIDS at the Victoria Hospital in the Alice Area of the province of the Eastern Cape. (iii) To identify possible psychosocial support systems that could be provided by social service practitioners and by social networks for people living with HIV and AIDS and their families. The study used qualitative research approach where in-depth interviews and a focus group with key informants were used as methods of data collection. The study was premised on the systems theory to give a theoretical orientation into understanding the psychosocial challenges of people living with HIV/AIDS and possible support to be given to them. The sample size was made up of 10 patient participants; eight (8) ii females, two (2) males; and three (3) health practitioners. Non-probability sampling technique was used in the study. Specifically, purposive sampling was used to select the participants because the researcher believed that the participants had knowledge and experiences about HIV/AIDS. The data was analysed qualitatively through a content thematic analysis technique. The study found that there are many psychosocial challenges that are faced by people living with HIV/AIDS such as poverty, social support, rejection, depression and stigma and discrimination. The study recommends that the affected communities should have support groups for people living with HIV/AIDS to empower them and their families. The study also recommends the implementation of community engagements in the form of educating communities about HIV/AIDS and the importance of supporting people living with HIV/AIDS. More emphasis on the right to equality and the right for everyone to be treated with dignity and respect, as stated by Chapter 2 of the Bill of Rights of the South African constitution is highly recommended. Based on the evidence provided through the findings, the researcher is optimistic that all the challenges presented will be taken into consideration in future plans and policies.
- Full Text:
- Date Issued: 2019
An in-silico investigation of Morita-Baylis-Hillman accessible heterocyclic analogues for applications as novel HIV-1 C protease inhibitors
- Authors: Sigauke, Lester Takunda
- Date: 2015
- Subjects: Protease inhibitors , Heterocyclic compounds , HIV (Viruses) , HIV infections , Drug resistance , Cheminformatics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4152 , http://hdl.handle.net/10962/d1017913
- Description: Cheminformatic approaches have been employed to optimize the bis-coumarin scaffold identified by Onywera et al. (2012) as a potential hit against the protease HIV-1 protein. The Open Babel library of commands was used to access functions that were incorporated into a markov chain recursive program that generated 17750 analogues of the bis-coumarin scaffold. The Morita-Baylis-Hillman accessible heterocycles were used to introduce structural diversity within the virtual library. In silico high through-put virtual screening using AutoDock Vina was used to rapidly screen the virtual library ligand set against 61 protease models built by Onywera et al. (2012). CheS-Mapper computed a principle component analysis of the compounds based on 13 selected chemical descriptors. The compounds were plotted against the principle component analysis within a 3 dimensional chemical space in order to inspect the diversity of the virtual library. The physicochemical properties and binding affinities were used to identify the top 3 performing ligands. ACPYPE was used to inspect the constitutional properties and eliminated virtual compounds that possessed open valences. Chromene based ligand 805 and ligand 6610 were selected as the lead candidates from the high-throughput virtual screening procedure we employed. Molecular dynamic simulations of the lead candidates performed for 5 ns allowed the stability of the ligand protein complexes with protease model 305152. The free energy of binding of the leads with protease model 305152 was computed over the first 50 ps of simulation using the molecular mechanics Poisson-Boltzmann method. Analysis structural features and energy profiles from molecular dynamic simulations of the protein–ligand complexes indicated that although ligand 805 had a weaker binding affinity in terms of docking, it outperformed ligand 6610 in terms of complex stability and free energy of binding. Medicinal chemistry approaches will be used to optimize the lead candidates before their analogues will be synthesized and assayed for in vivo protease activity.
- Full Text:
- Date Issued: 2015
- Authors: Sigauke, Lester Takunda
- Date: 2015
- Subjects: Protease inhibitors , Heterocyclic compounds , HIV (Viruses) , HIV infections , Drug resistance , Cheminformatics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4152 , http://hdl.handle.net/10962/d1017913
- Description: Cheminformatic approaches have been employed to optimize the bis-coumarin scaffold identified by Onywera et al. (2012) as a potential hit against the protease HIV-1 protein. The Open Babel library of commands was used to access functions that were incorporated into a markov chain recursive program that generated 17750 analogues of the bis-coumarin scaffold. The Morita-Baylis-Hillman accessible heterocycles were used to introduce structural diversity within the virtual library. In silico high through-put virtual screening using AutoDock Vina was used to rapidly screen the virtual library ligand set against 61 protease models built by Onywera et al. (2012). CheS-Mapper computed a principle component analysis of the compounds based on 13 selected chemical descriptors. The compounds were plotted against the principle component analysis within a 3 dimensional chemical space in order to inspect the diversity of the virtual library. The physicochemical properties and binding affinities were used to identify the top 3 performing ligands. ACPYPE was used to inspect the constitutional properties and eliminated virtual compounds that possessed open valences. Chromene based ligand 805 and ligand 6610 were selected as the lead candidates from the high-throughput virtual screening procedure we employed. Molecular dynamic simulations of the lead candidates performed for 5 ns allowed the stability of the ligand protein complexes with protease model 305152. The free energy of binding of the leads with protease model 305152 was computed over the first 50 ps of simulation using the molecular mechanics Poisson-Boltzmann method. Analysis structural features and energy profiles from molecular dynamic simulations of the protein–ligand complexes indicated that although ligand 805 had a weaker binding affinity in terms of docking, it outperformed ligand 6610 in terms of complex stability and free energy of binding. Medicinal chemistry approaches will be used to optimize the lead candidates before their analogues will be synthesized and assayed for in vivo protease activity.
- Full Text:
- Date Issued: 2015
Application of Baylis-Hillman methodology in the synthesis of HIV-1 enzyme inhibitors
- Authors: Manyeruke, Meloddy Hlatini
- Date: 2015
- Subjects: HIV infections , Enzyme inhibitors , AZT (Drug) , Bioconjugates
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4540 , http://hdl.handle.net/10962/d1017920
- Description: The application of Baylis-Hillman methodology has afforded access to a range of β-hydroxypropionate ester-AZT conjugates as potential dual-action HIV-1 IN/RT inhibitors. Two families comprising a total of nine β-hydroxypropionate ester-AZT conjugates were synthesised. The first family was accessed using O-benzylated salicylaldehydes and methyl acrylate and the second from unprotected salicylaldehydes using tert-butyl acrylate as the activated alkene. Spectroscopic methods were employed to fully characterize the compounds. Propargylation of the respective Baylis-Hillman adducts was achieved via conjugate addition of propargylamine. The resulting products were then employed in Cu(I)-catalysed “click” reactions with azidothymidine (AZT) to yield the desired β-hydroxypropionate ester-AZT conjugates. Exploratory studies were also conducted to access 4-hydroxycoumarins from Baylis-Hillman derived adducts and to construct customized chiral Baylis-Hillman reaction sites. Many 4- hydroxycoumarins are known to exhibit a wide range of biological activities, and extending Baylis-Hillman methodology to access these systems is an important challenge. Two approaches were investigated. The first involved the formation of a 4-phthalimidocoumarin, aromatisation and hydrolysis of which was expected to lead to the 4-hydroxycoumarin target. The second, a variation of the first, involved the use of 4-(chrolomethyl)coumarin intermediates. Unfortunately, while various intermediates were prepared and characterised, neither approach led ultimately to the desired targets. N-substituted borneol-10-sulfonamides were constructed from camphor-10- sulfonyl chloride as chiral Baylis-Hillman reaction sites. In a preliminary study, however, none of the N-substituted borneol-10-sulfonamides exhibited Baylis-Hillman catalytic activity.
- Full Text:
- Date Issued: 2015
- Authors: Manyeruke, Meloddy Hlatini
- Date: 2015
- Subjects: HIV infections , Enzyme inhibitors , AZT (Drug) , Bioconjugates
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4540 , http://hdl.handle.net/10962/d1017920
- Description: The application of Baylis-Hillman methodology has afforded access to a range of β-hydroxypropionate ester-AZT conjugates as potential dual-action HIV-1 IN/RT inhibitors. Two families comprising a total of nine β-hydroxypropionate ester-AZT conjugates were synthesised. The first family was accessed using O-benzylated salicylaldehydes and methyl acrylate and the second from unprotected salicylaldehydes using tert-butyl acrylate as the activated alkene. Spectroscopic methods were employed to fully characterize the compounds. Propargylation of the respective Baylis-Hillman adducts was achieved via conjugate addition of propargylamine. The resulting products were then employed in Cu(I)-catalysed “click” reactions with azidothymidine (AZT) to yield the desired β-hydroxypropionate ester-AZT conjugates. Exploratory studies were also conducted to access 4-hydroxycoumarins from Baylis-Hillman derived adducts and to construct customized chiral Baylis-Hillman reaction sites. Many 4- hydroxycoumarins are known to exhibit a wide range of biological activities, and extending Baylis-Hillman methodology to access these systems is an important challenge. Two approaches were investigated. The first involved the formation of a 4-phthalimidocoumarin, aromatisation and hydrolysis of which was expected to lead to the 4-hydroxycoumarin target. The second, a variation of the first, involved the use of 4-(chrolomethyl)coumarin intermediates. Unfortunately, while various intermediates were prepared and characterised, neither approach led ultimately to the desired targets. N-substituted borneol-10-sulfonamides were constructed from camphor-10- sulfonyl chloride as chiral Baylis-Hillman reaction sites. In a preliminary study, however, none of the N-substituted borneol-10-sulfonamides exhibited Baylis-Hillman catalytic activity.
- Full Text:
- Date Issued: 2015
Structural studies on yeast eIF5A using biomolecular NMR and molecular dynamics
- Authors: Sigauke, Lester Takunda
- Date: 2015
- Subjects: Molecular dynamics , Reverse transcriptase , HIV (Viruses) , HIV infections , Eukaryotic cells , Yeast
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4547 , http://hdl.handle.net/10962/d1017927
- Description: Eukaryotic initiation factor 5A, eIF5A, is a ubiquitous eukaryotic protein that has been shown to influence the translation initiation of a specific subset of mRNAs. It is the only protein known to undergo hypusination in a two-step post translational modification process involving deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH) enzymes. Hypusination has been shown to influence translation of HIV-1 and HTLV-1 nuclear export signals, while the involvement of active hypusinated eIF5A in induction of IRES mediated processes that initiate pro-apoptotic process have inspired studies into the manipulation of eIF5A in anti-cancer and anti-diabetic therapies. eIF5A oligomerisation in eukaryotic systems has been shown to be influenced by hypusination and the mechanism of dimerisation is RNA dependent. Nuclear magnetic resonance spectroscopy approaches were proposed to solve the structure of the hypusinated eIF5A in solution in order to understand the influence of hypusination on the monomeric arrangement which enhances dimerisation and activates the protein. Cleavage of the 18 kDa protein monomer by introduction of thrombin cleavage site within the flexible domain was thought to give rise to 10 kDa fragments accessible to a 600 MHz NMR spectrometer. Heteronuclear single quantum correlation experiments of the mutated isotopically labelled protein expressed in E. coli showed that the eIF5A protein with a thrombin cleavage insert, eIF5AThr (eIF5A subscript Thr), was unfolded. In silico investigations of the behaviour of eIF5A and eIF5AThr (eIF5A subscript Thr) models in solution using molecular dynamics showed that the mutated model had different solution dynamics to the native model. Chemical shift predictors were used to extract atomic resolution data of solution dynamics and the introduction of rigidity in the flexible loop region of eIF5A affected solution behaviour consistent with lack of in vivo function of eIF5AThr (eIF5A subscript Thr) in yeast. Residual dipolar coupling and T₁ relaxation times were calculated in anticipation of the extraction of experimental data from RDC and relaxation dispersion experiments based on HSQC measurable restraints.
- Full Text:
- Date Issued: 2015
- Authors: Sigauke, Lester Takunda
- Date: 2015
- Subjects: Molecular dynamics , Reverse transcriptase , HIV (Viruses) , HIV infections , Eukaryotic cells , Yeast
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4547 , http://hdl.handle.net/10962/d1017927
- Description: Eukaryotic initiation factor 5A, eIF5A, is a ubiquitous eukaryotic protein that has been shown to influence the translation initiation of a specific subset of mRNAs. It is the only protein known to undergo hypusination in a two-step post translational modification process involving deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH) enzymes. Hypusination has been shown to influence translation of HIV-1 and HTLV-1 nuclear export signals, while the involvement of active hypusinated eIF5A in induction of IRES mediated processes that initiate pro-apoptotic process have inspired studies into the manipulation of eIF5A in anti-cancer and anti-diabetic therapies. eIF5A oligomerisation in eukaryotic systems has been shown to be influenced by hypusination and the mechanism of dimerisation is RNA dependent. Nuclear magnetic resonance spectroscopy approaches were proposed to solve the structure of the hypusinated eIF5A in solution in order to understand the influence of hypusination on the monomeric arrangement which enhances dimerisation and activates the protein. Cleavage of the 18 kDa protein monomer by introduction of thrombin cleavage site within the flexible domain was thought to give rise to 10 kDa fragments accessible to a 600 MHz NMR spectrometer. Heteronuclear single quantum correlation experiments of the mutated isotopically labelled protein expressed in E. coli showed that the eIF5A protein with a thrombin cleavage insert, eIF5AThr (eIF5A subscript Thr), was unfolded. In silico investigations of the behaviour of eIF5A and eIF5AThr (eIF5A subscript Thr) models in solution using molecular dynamics showed that the mutated model had different solution dynamics to the native model. Chemical shift predictors were used to extract atomic resolution data of solution dynamics and the introduction of rigidity in the flexible loop region of eIF5A affected solution behaviour consistent with lack of in vivo function of eIF5AThr (eIF5A subscript Thr) in yeast. Residual dipolar coupling and T₁ relaxation times were calculated in anticipation of the extraction of experimental data from RDC and relaxation dispersion experiments based on HSQC measurable restraints.
- Full Text:
- Date Issued: 2015
Indian secondary school youths' understanding of sexual violence in their community in the age of HIV and AIDS: a participatory video approach
- Authors: Mahadev, Rekha
- Date: 2014
- Subjects: Sex crimes , Indian youth -- Education (Secondary) , AIDS (Disease) , HIV infections
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/7522 , vital:21793
- Description: This study focuses on Indian secondary school youths’ understanding of sexual violence in their community in the age of HIV and AIDS, and the contribution that participatory video can make to address sexual violence. South Africa, apart from having the highest HIV prevalence in the world, also has the highest incidence of sexual violence. South African society often appears complacent about the high levels of sexual violence. In the Indian community, especially among the youth, sexual violence is also cause for concern. Much effort and energy has been expended in educating learners about sexual violence and HIV and AIDS with the objective of raising awareness of the dangers of engaging in risky sexual behaviour, and ultimately to empower and influence positive behaviour change. Because HIV prevalence in the Indian population is on the rise, Indian youth’s particular vulnerability is the reason for focusing on how they understand sexual violence in the age of AIDS. Besides the paucity of research on Indian youth and sexual violence, the methodologies which have been used produced research which is descriptive in nature and hence a methodological shift from traditional methodologies to a participatory visual methodology which has the potential for critically engaging the Indian youth on the issues of sexual violence, could contribute to research which has a social change focus. This qualitative research therefore uses a visual participatory methodology within a critical research paradigm, to explore and contribute to addressing the problem of sexual violence in the age of HIV and AIDS. The participants in the study, a sample of 20 Indian learners (10 boys and 10 girls), from a secondary school in Durban which has predominantly Indian learners, was purposively selected from Grade 11 classes. Participatory video enabled them to voice how they understand sexual violence and in doing so move towards reflecting on their own agency. The theory of triadic influence with its three streams, i.e. cultural attitudinal, social normative and intrapersonal, was used to make meaning of the findings. It also provided a frame for a fourth path, namely the preventive intervene. While participatory video enabled exploring Indian youths’ understanding of sexual violence in their community in the age of AIDS, it at the same time enabled them to reflect on, and perhaps begin to disrupt their understanding of the cultural attitudinal, social normative and the intrapersonal influences and how these influence their thinking about sexual violence. Two a priori themes were established prior to the analysis, to respond to how Indian youths understand sexual violence in the age of AIDS, and how participatory video can address sexual violence. The findings suggest that their understanding of sexual violence stems from a culture of concealment in which veiling sexual violence is the norm; that the vulnerability of youth increases as the experience of pressure from peers to engage in sexual violence increases; and that sexual violence is traumatising. The use of participatory video increased the youths’ reflexivity and created a space for them to explore how to take action. The findings imply that addressing sexual violence with Indian youth should begin with interrogating the cultural norms of masculinities and femininities, and the cultural practices rooted in traditional structures of the family and community which perpetuate gender disparities and the restriction of the autonomy of women and girls. Addressing issues of vulnerability and sexual violence should be the focus of all school and community interventions to ensure learners’ well-being and ability to resist negative peer pressure. These interventions should encourage self-reflection and raise social awareness through active participation and in so doing bring about social change in the Indian community. Vigorous participatory interventions which draw on the voices of the Indian youth as agents of social change in addressing sexual violence in the age of HIV and AIDS, is therefore urgent. The significance of this study as research as intervention is useful in enabling the exploration - with the Indian youth - of how cultural and religious norms, gender disparity, Indian masculinities and femininities, and social peer pressure feed into youth’s understanding of sexual violence and at the same time getting them to begin rethinking, challenging and disrupting these understandings where necessary. The study demonstrates that engaging youth affords them an opportunity to draw from their own experiences and through their own voices and actions create knowledge through participatory video, thereby making a contribution to visual methodologies and research around sexual violence and HIV and AIDS in their world.
- Full Text:
- Date Issued: 2014
- Authors: Mahadev, Rekha
- Date: 2014
- Subjects: Sex crimes , Indian youth -- Education (Secondary) , AIDS (Disease) , HIV infections
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/7522 , vital:21793
- Description: This study focuses on Indian secondary school youths’ understanding of sexual violence in their community in the age of HIV and AIDS, and the contribution that participatory video can make to address sexual violence. South Africa, apart from having the highest HIV prevalence in the world, also has the highest incidence of sexual violence. South African society often appears complacent about the high levels of sexual violence. In the Indian community, especially among the youth, sexual violence is also cause for concern. Much effort and energy has been expended in educating learners about sexual violence and HIV and AIDS with the objective of raising awareness of the dangers of engaging in risky sexual behaviour, and ultimately to empower and influence positive behaviour change. Because HIV prevalence in the Indian population is on the rise, Indian youth’s particular vulnerability is the reason for focusing on how they understand sexual violence in the age of AIDS. Besides the paucity of research on Indian youth and sexual violence, the methodologies which have been used produced research which is descriptive in nature and hence a methodological shift from traditional methodologies to a participatory visual methodology which has the potential for critically engaging the Indian youth on the issues of sexual violence, could contribute to research which has a social change focus. This qualitative research therefore uses a visual participatory methodology within a critical research paradigm, to explore and contribute to addressing the problem of sexual violence in the age of HIV and AIDS. The participants in the study, a sample of 20 Indian learners (10 boys and 10 girls), from a secondary school in Durban which has predominantly Indian learners, was purposively selected from Grade 11 classes. Participatory video enabled them to voice how they understand sexual violence and in doing so move towards reflecting on their own agency. The theory of triadic influence with its three streams, i.e. cultural attitudinal, social normative and intrapersonal, was used to make meaning of the findings. It also provided a frame for a fourth path, namely the preventive intervene. While participatory video enabled exploring Indian youths’ understanding of sexual violence in their community in the age of AIDS, it at the same time enabled them to reflect on, and perhaps begin to disrupt their understanding of the cultural attitudinal, social normative and the intrapersonal influences and how these influence their thinking about sexual violence. Two a priori themes were established prior to the analysis, to respond to how Indian youths understand sexual violence in the age of AIDS, and how participatory video can address sexual violence. The findings suggest that their understanding of sexual violence stems from a culture of concealment in which veiling sexual violence is the norm; that the vulnerability of youth increases as the experience of pressure from peers to engage in sexual violence increases; and that sexual violence is traumatising. The use of participatory video increased the youths’ reflexivity and created a space for them to explore how to take action. The findings imply that addressing sexual violence with Indian youth should begin with interrogating the cultural norms of masculinities and femininities, and the cultural practices rooted in traditional structures of the family and community which perpetuate gender disparities and the restriction of the autonomy of women and girls. Addressing issues of vulnerability and sexual violence should be the focus of all school and community interventions to ensure learners’ well-being and ability to resist negative peer pressure. These interventions should encourage self-reflection and raise social awareness through active participation and in so doing bring about social change in the Indian community. Vigorous participatory interventions which draw on the voices of the Indian youth as agents of social change in addressing sexual violence in the age of HIV and AIDS, is therefore urgent. The significance of this study as research as intervention is useful in enabling the exploration - with the Indian youth - of how cultural and religious norms, gender disparity, Indian masculinities and femininities, and social peer pressure feed into youth’s understanding of sexual violence and at the same time getting them to begin rethinking, challenging and disrupting these understandings where necessary. The study demonstrates that engaging youth affords them an opportunity to draw from their own experiences and through their own voices and actions create knowledge through participatory video, thereby making a contribution to visual methodologies and research around sexual violence and HIV and AIDS in their world.
- Full Text:
- Date Issued: 2014
Management of HIV/AIDS programmes at the workplace: a study of selected organisations in Chris Hani District, Eastern Cape Province
- Authors: Shava, Getrude
- Date: 2013-04
- Subjects: HIV infections
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24834 , vital:63601
- Description: The aim of the study was to investigate the management of HIV /AIDS programmes at the workplace in four selected organisations in Chris Hani District, Eastern Cape Province of South Africa. Four organisations were studied, two public organisations and two private organisations. With the use of triangulation method, two hundred employees were administered a semi- structured questionnaire while for (four) managers, semi structured in-depth interviews were conducted. The major findings of this study outline that all the four organisations studied have HIV/AIDS programmes and policies for their employees. However, there were no budget allocations for these programmes to be fully implemented for effectiveness. From the data, it can be concluded that HIV/AIDS has a negative impact on organisations‟ production like high training costs, high labour turnover and high absenteeism from work. This has been as a result of managers who did not put their total commitment towards HIV/AIDS management at their workplaces in the same way they have done to other core areas of businesses of their organisations. The study therefore recommends the management of these organisations to demonstrate a clear commitment to the HIV/AIDS management strategies by fully implementing the HIV/AIDS management programmes in their workplaces. It is very crucial for employees to see this commitment in a concrete form through non-discrimination and support for the people living with HIV/AIDS. Clear unambiguous commitment will go far in developing mutual trust between employers and employees and facilitate an atmosphere where people are willing to undergo VCT and to possibly disclose their status. Furthermore, managers are recommended to hire quality service providers to carry out intensive de-stigmatisation processes. This will create a supportive environment and adequately address the fears of employees about HIV/AIDS issues at the workplace. All these recommendations will go a long way in assisting organisations achieve their strategic business objectives and reduce the negative impact of HIV/AIDS at their workplaces. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2013
- Full Text:
- Date Issued: 2013-04
- Authors: Shava, Getrude
- Date: 2013-04
- Subjects: HIV infections
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24834 , vital:63601
- Description: The aim of the study was to investigate the management of HIV /AIDS programmes at the workplace in four selected organisations in Chris Hani District, Eastern Cape Province of South Africa. Four organisations were studied, two public organisations and two private organisations. With the use of triangulation method, two hundred employees were administered a semi- structured questionnaire while for (four) managers, semi structured in-depth interviews were conducted. The major findings of this study outline that all the four organisations studied have HIV/AIDS programmes and policies for their employees. However, there were no budget allocations for these programmes to be fully implemented for effectiveness. From the data, it can be concluded that HIV/AIDS has a negative impact on organisations‟ production like high training costs, high labour turnover and high absenteeism from work. This has been as a result of managers who did not put their total commitment towards HIV/AIDS management at their workplaces in the same way they have done to other core areas of businesses of their organisations. The study therefore recommends the management of these organisations to demonstrate a clear commitment to the HIV/AIDS management strategies by fully implementing the HIV/AIDS management programmes in their workplaces. It is very crucial for employees to see this commitment in a concrete form through non-discrimination and support for the people living with HIV/AIDS. Clear unambiguous commitment will go far in developing mutual trust between employers and employees and facilitate an atmosphere where people are willing to undergo VCT and to possibly disclose their status. Furthermore, managers are recommended to hire quality service providers to carry out intensive de-stigmatisation processes. This will create a supportive environment and adequately address the fears of employees about HIV/AIDS issues at the workplace. All these recommendations will go a long way in assisting organisations achieve their strategic business objectives and reduce the negative impact of HIV/AIDS at their workplaces. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2013
- Full Text:
- Date Issued: 2013-04
Understanding the sexual risk behaviours of people living with different mental illnesses and their vulnerability to HIV infection
- Authors: Ndlovu, Deogracious
- Date: 2013
- Subjects: Health -- Social aspects , HIV infections
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/25037 , vital:63899
- Description: This study was primarily carried out in order to assess the sexual risk behaviours of people living with mental illnesses as well as their vulnerability to HIV infection. It sets out the basic realities of the interaction between mental illness and HIV/AIDS and its impact on individuals and communities. This study explores the implications for health policy and practice, and suggests how to provide better support and assistance in the empowerment of people living with mental illness as far as HIV and AIDS is concerned, including community support. It was also the aim of this study to look into the factors that expose this group of people to HIV infection as well as to understand the link between HIV/AIDS and mental illness. It also tested the inclusion of people with mental disabilities in HIV and AIDS prevention and education programmes. The research was conducted on the basis of a qualitative research design. A semi-structured interview schedule was used in broad based interviews to gather information from caregivers or guardians of people with mental disabilities as well as the professionals in the mental health sector. The results of the research revealed that people who are living with mental illnesses are a vulnerable group as regards HIV infection. It also conveys that this group of people presents with sexual risk behaviours, which increase their vulnerability to HIV and AIDS. In addition, there are many barriers against the inclusion of this group of people in HIV and AIDS prevention and education programmes and, as a result, they are often excluded from participating in such programmes. Importantly, the findings of this study acknowledge that there is a two-fold relationship between mental illness and HIV/AIDS. It indicates that mental illnesses are still regarded as mysterious in the community and there is much stigma and discrimination towards the mentally ill, increasing their vulnerability to HIV infection. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2013
- Full Text:
- Date Issued: 2013
- Authors: Ndlovu, Deogracious
- Date: 2013
- Subjects: Health -- Social aspects , HIV infections
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/25037 , vital:63899
- Description: This study was primarily carried out in order to assess the sexual risk behaviours of people living with mental illnesses as well as their vulnerability to HIV infection. It sets out the basic realities of the interaction between mental illness and HIV/AIDS and its impact on individuals and communities. This study explores the implications for health policy and practice, and suggests how to provide better support and assistance in the empowerment of people living with mental illness as far as HIV and AIDS is concerned, including community support. It was also the aim of this study to look into the factors that expose this group of people to HIV infection as well as to understand the link between HIV/AIDS and mental illness. It also tested the inclusion of people with mental disabilities in HIV and AIDS prevention and education programmes. The research was conducted on the basis of a qualitative research design. A semi-structured interview schedule was used in broad based interviews to gather information from caregivers or guardians of people with mental disabilities as well as the professionals in the mental health sector. The results of the research revealed that people who are living with mental illnesses are a vulnerable group as regards HIV infection. It also conveys that this group of people presents with sexual risk behaviours, which increase their vulnerability to HIV and AIDS. In addition, there are many barriers against the inclusion of this group of people in HIV and AIDS prevention and education programmes and, as a result, they are often excluded from participating in such programmes. Importantly, the findings of this study acknowledge that there is a two-fold relationship between mental illness and HIV/AIDS. It indicates that mental illnesses are still regarded as mysterious in the community and there is much stigma and discrimination towards the mentally ill, increasing their vulnerability to HIV infection. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2013
- Full Text:
- Date Issued: 2013
Evaluation of the role of support groups in the lives of HIV positive people at Nontyatyambo and Empilweni Gompo Community Health Centres in East London, Eastern Cape
- Mkhencele, Nontando Precious
- Authors: Mkhencele, Nontando Precious
- Date: 2011
- Subjects: HIV-positive persons , Self-help groups -- South Africa -- Eastern Cape , HIV infections , AIDS (Disease) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Nursing Science)
- Identifier: vital:11894 , http://hdl.handle.net/10353/d1001094 , HIV-positive persons , Self-help groups -- South Africa -- Eastern Cape , HIV infections , AIDS (Disease) -- South Africa -- Eastern Cape
- Description: South Africa has the highest number of people living with HIV/AIDS in the world. The estimated 5,7 million South Africans that are living with HIV need comprehensive and holistic care. Psychosocial support is a vital aspect of care for HIV positive people. Support groups have been identified as a basic form of psychosocial support. The aim of this study was to evaluate the role of support groups in the lives of HIV positive people in East London, Eastern Cape. A qualitative study design was implemented using focus group interviews to explore the role of HIV support groups. The research questions were designed to elicit responses pertaining to the needs, expectations and experiences of HIV positive support group attendees. Activities conducted in support groups as well as the attitude of support group members towards recruiting other HIV positive people to join the group were also explored. Findings revealed that the benefits of attending a support group included emotional and psychological support, sense of belonging to a “family”, assistance with disclosure issues, gaining information about HIV and treatment as well as material benefits such as food parcels and job opportunities. The greatest need of support group attendees was assistance in obtaining a Social Support or Disability Grant. A few negative experiences were reported which included: unfulfilled promises by people outside of the group, unfair allocation of grants and food parcels, as well as negative group dynamics at times. Support group members agreed that even though there were few negative experiences, the benefits clearly outweighed the negative experiences. Most participants agreed that they would recommend the support group to other HIV positive people so that they could also enjoy the stated benefits. In summary, the study concluded that support groups are very helpful in the lives of HIV positive people.
- Full Text:
- Date Issued: 2011
- Authors: Mkhencele, Nontando Precious
- Date: 2011
- Subjects: HIV-positive persons , Self-help groups -- South Africa -- Eastern Cape , HIV infections , AIDS (Disease) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Nursing Science)
- Identifier: vital:11894 , http://hdl.handle.net/10353/d1001094 , HIV-positive persons , Self-help groups -- South Africa -- Eastern Cape , HIV infections , AIDS (Disease) -- South Africa -- Eastern Cape
- Description: South Africa has the highest number of people living with HIV/AIDS in the world. The estimated 5,7 million South Africans that are living with HIV need comprehensive and holistic care. Psychosocial support is a vital aspect of care for HIV positive people. Support groups have been identified as a basic form of psychosocial support. The aim of this study was to evaluate the role of support groups in the lives of HIV positive people in East London, Eastern Cape. A qualitative study design was implemented using focus group interviews to explore the role of HIV support groups. The research questions were designed to elicit responses pertaining to the needs, expectations and experiences of HIV positive support group attendees. Activities conducted in support groups as well as the attitude of support group members towards recruiting other HIV positive people to join the group were also explored. Findings revealed that the benefits of attending a support group included emotional and psychological support, sense of belonging to a “family”, assistance with disclosure issues, gaining information about HIV and treatment as well as material benefits such as food parcels and job opportunities. The greatest need of support group attendees was assistance in obtaining a Social Support or Disability Grant. A few negative experiences were reported which included: unfulfilled promises by people outside of the group, unfair allocation of grants and food parcels, as well as negative group dynamics at times. Support group members agreed that even though there were few negative experiences, the benefits clearly outweighed the negative experiences. Most participants agreed that they would recommend the support group to other HIV positive people so that they could also enjoy the stated benefits. In summary, the study concluded that support groups are very helpful in the lives of HIV positive people.
- Full Text:
- Date Issued: 2011
Exploring ways of assisting Lesotho educators to offer care and support to children orphaned and rendered vulnerable by HIV and AIDS
- Authors: Ntaote, Grace Makeletso
- Date: 2011
- Subjects: Orphans -- Developing Countries -- Social conditions , HIV infections
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9503 , http://hdl.handle.net/10948/1320 , Orphans -- Developing Countries -- Social conditions , HIV infections
- Description: The HIV and AIDS pandemic has resulted in 14 million children being orphaned worldwide. In Lesotho alone, where this study was carried out, there are about 180 000 of these children (UNAIDS, 2007). Teachers, especially in Lesotho‘s primary schools need to be equipped to better deal with the challenges that result from having these children in their classrooms. At the Lesotho College of Education, where I have worked for 12 years as a teacher educator, pre-service and in-service student teachers are not trained to offer care and support to orphans and vulnerable children. They experience problems in the classroom emanating from the needs of these children. This study followed an action research design to find ways to support teachers to better deal with the issues they face as a result of having orphans and vulnerable children in their classes. Using a qualitative approach, educators perceptions, feelings, attitudes and experiences in dealing with orphans and vulnerable children in their schools were identified, and it became apparent that educators were negatively affected on a personal and professional level. It was concluded that the development of resilience in educators would help them to better cope with orphans and vulnerable children in their classes. The chosen intervention Resilient Educators Programme (REds) was implemented and evaluated and findings revealed that it was beneficial in increasing educator resilience. Recommendations, based on the findings of the study, were made for future teacher education in this area.
- Full Text:
- Date Issued: 2011
- Authors: Ntaote, Grace Makeletso
- Date: 2011
- Subjects: Orphans -- Developing Countries -- Social conditions , HIV infections
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9503 , http://hdl.handle.net/10948/1320 , Orphans -- Developing Countries -- Social conditions , HIV infections
- Description: The HIV and AIDS pandemic has resulted in 14 million children being orphaned worldwide. In Lesotho alone, where this study was carried out, there are about 180 000 of these children (UNAIDS, 2007). Teachers, especially in Lesotho‘s primary schools need to be equipped to better deal with the challenges that result from having these children in their classrooms. At the Lesotho College of Education, where I have worked for 12 years as a teacher educator, pre-service and in-service student teachers are not trained to offer care and support to orphans and vulnerable children. They experience problems in the classroom emanating from the needs of these children. This study followed an action research design to find ways to support teachers to better deal with the issues they face as a result of having orphans and vulnerable children in their classes. Using a qualitative approach, educators perceptions, feelings, attitudes and experiences in dealing with orphans and vulnerable children in their schools were identified, and it became apparent that educators were negatively affected on a personal and professional level. It was concluded that the development of resilience in educators would help them to better cope with orphans and vulnerable children in their classes. The chosen intervention Resilient Educators Programme (REds) was implemented and evaluated and findings revealed that it was beneficial in increasing educator resilience. Recommendations, based on the findings of the study, were made for future teacher education in this area.
- Full Text:
- Date Issued: 2011
The development, implementation and evaluation of a locus of control-based training programme for HIV and AIDS risk reduction among university students
- Authors: Gwandure, Calvin
- Date: 2009
- Subjects: AIDS (Disease) -- Prevention , HIV infections , Universities and colleges -- Health promotion services , Risk communication -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9838 , http://hdl.handle.net/10948/1242 , AIDS (Disease) -- Prevention , HIV infections , Universities and colleges -- Health promotion services , Risk communication -- South Africa
- Description: There is an escalation of HIV and AIDS among the youth in South Africa and other developing countries. Research on HIV and AIDS risk factors has tended to focus more on poverty, gender, race, illiteracy, and violence than personality factors that could influence an individual` s health-protective behaviour. Previous studies have also shown that wealth, education, race, and gender may not make an individual more or less vulnerable to HIV infection. This study argued that locus of control could influence an individual` s health-protective behaviour and that external locus of control could be a risk factor in HIV and AIDS risk reduction. The aim of this study was to investigate the efficacy of a locus of control-based training programme in reducing HIV and AIDS risk among university students. The locus of control-based variables that formed the training programme for HIV and AIDS risk reduction among university students were: social systems control, self-control, fatalism, achievement-oriented behaviour, deferment of gratification, personal values and expectancies, and social alienation. These locus of control-based variables were regarded as contexts in which individuals could exhibit health risk behaviours. A sample of 257 first-year university students participated in the study. There were (N = 170) female participants and (N = 87) male participants drawn from the University of the Witwatersrand. The study was a pretest-posttest repeated measures design. Data were analysed using t tests, correlations, multiple regression, structural equation modelling, and repeated measures tests. The results of this study showed significant differences in health risks between participants with an external locus of control and participants with an internal locus of control. There was a significant relationship between locus of control-based variables and HIV and AIDS risk. The locus of control-based training programme significantly modified personality and significantly reduced locus of control-based health risks and HIV and AIDS risk. Directions for future research on locus of control, health risks, and HIV and AIDS risk could focus on the development and implementation of various locus of control-based training programmes in South Africa. Locus of control should be targeted as a health risk factor in HIV and AIDS risk reduction training programmes.
- Full Text:
- Date Issued: 2009
- Authors: Gwandure, Calvin
- Date: 2009
- Subjects: AIDS (Disease) -- Prevention , HIV infections , Universities and colleges -- Health promotion services , Risk communication -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9838 , http://hdl.handle.net/10948/1242 , AIDS (Disease) -- Prevention , HIV infections , Universities and colleges -- Health promotion services , Risk communication -- South Africa
- Description: There is an escalation of HIV and AIDS among the youth in South Africa and other developing countries. Research on HIV and AIDS risk factors has tended to focus more on poverty, gender, race, illiteracy, and violence than personality factors that could influence an individual` s health-protective behaviour. Previous studies have also shown that wealth, education, race, and gender may not make an individual more or less vulnerable to HIV infection. This study argued that locus of control could influence an individual` s health-protective behaviour and that external locus of control could be a risk factor in HIV and AIDS risk reduction. The aim of this study was to investigate the efficacy of a locus of control-based training programme in reducing HIV and AIDS risk among university students. The locus of control-based variables that formed the training programme for HIV and AIDS risk reduction among university students were: social systems control, self-control, fatalism, achievement-oriented behaviour, deferment of gratification, personal values and expectancies, and social alienation. These locus of control-based variables were regarded as contexts in which individuals could exhibit health risk behaviours. A sample of 257 first-year university students participated in the study. There were (N = 170) female participants and (N = 87) male participants drawn from the University of the Witwatersrand. The study was a pretest-posttest repeated measures design. Data were analysed using t tests, correlations, multiple regression, structural equation modelling, and repeated measures tests. The results of this study showed significant differences in health risks between participants with an external locus of control and participants with an internal locus of control. There was a significant relationship between locus of control-based variables and HIV and AIDS risk. The locus of control-based training programme significantly modified personality and significantly reduced locus of control-based health risks and HIV and AIDS risk. Directions for future research on locus of control, health risks, and HIV and AIDS risk could focus on the development and implementation of various locus of control-based training programmes in South Africa. Locus of control should be targeted as a health risk factor in HIV and AIDS risk reduction training programmes.
- Full Text:
- Date Issued: 2009
Prevalence of Escherichia coli O157:H7 in water and meat and meat products and vegetables sold in the Eastern Cape Province of South Africa and its impact on the diarrhoeic conditions of HIV/AIDS patients
- Authors: Abong'o, Benard Omondi
- Date: 2008
- Subjects: Foodborne diseases , Diarrhea , Escherichia coli , HIV infections , AIDS (Disease) , Bacterial diseases
- Language: English
- Type: Thesis , Doctoral , PhD (Microbiology)
- Identifier: vital:11263 , http://hdl.handle.net/10353/87 , Foodborne diseases , Diarrhea , Escherichia coli , HIV infections , AIDS (Disease) , Bacterial diseases
- Description: Water and food borne Escherichia coli O157:H7 could be one of the pathogens posing high health risk to patients suffering from Acquired Immunodeficiency Syndrome (AIDS) because of its incrimination in diarrhoea cases in AIDS patients. The present study, which was conducted between March 2005 and August 2006, investigated the prevalence of E. coli O157:H7 in water, meat and meat products and vegetables and its impact on diarrhoeic conditions of confirmed and non-confirmed HIV/AIDS patients in the Amathole District in the Eastern Cape Province of South Africa. The water samples used in the study were obtained from stand pipes supplying treated drinking water to communities residing in Fort Beaufort, Alice, Dimbaza and Mdantsane whereas borehole waters were sampled from Ngwenya and Kwasaki. The meat and meat products and vegetable samples were purchased from shops, butcheries, supermarkets and open air markets in Fort Beaufort, Alice and Mdantsane. The stool swabs used in the study were obtained from HIV/AIDS and outpatient clinics at Frere Hospital in East London. A total of 180 each of water, meat and meat products and vegetable samples and another 360 stool samples were analyzed for E. coli O157:H7. Presumptive E. coli O157 was isolated from the samples by culture-based methods and confirmed using Polymerase Chain Reaction techniques. Anti-biogram as well as risk assessment were also carried out using standard methods. The viable counts of presumptive E. coli O157 for water samples ranged between 3.3 × 104 and 1.71 × 105 CFU/ml, and between 1.8 × 104 and 5.04 × 106 CFU/g for meat and meat products, whereas those for vegetables ranged between 1.3 × 103 and 1.6 × 106 CFU/g. The counts of presumptive E. coli O157 for the water and vegetable samples were not significantly different whereas those for meat and meat products were found to be significantly different (P ≤ 0.05). The prevalence rates of presumptive E coli O157 in meat and meat products was 35.55 percent (64/180), and 25.55 percent (46/180) and 21.66 percent (39/180) for water and vegetables respectively. Prevalence of presumptive E. coli O157 in the stool samples of HIV/AIDS patients was 36.39 percent (131/360), of which 56.5 percent (74/131) and 43.5 percent (57/131) were from stools of confirmed and non-confirmed HIV/AIDS patients, respectively. Molecular analysis of representative presumptive E. coli O157 indicated that 10.29 percent (4/39) of vegetables; 14.81 percent (4/27) of water and 38.46 percent (5/13) of meat and meat products carried E. coli O157:H7. Also 36 percent (9/25) and 17.24 percent (5/29) of the stool samples were positive for E. coli O157:H7. Antimicrobial susceptibility profile revealed that all of the E. coli O157:H7 isolated from water, meat and meat products and vegetables as well as those isolated from stools of confirmed and non-confirmed HIV/AIDS patients were resistant (R) to gentamycin and erythromycin. However, 75 percent (20/27) of these isolates were resistant (R) to ampicillin and tetracycline whereas approximately 25 percent (6/27) were resistant (R) to nalidixic acid, ceftriaxone, and chloramphenicol. All the isolates (27/27) were susceptible (S) to amikacin. Probability of risk of E. coli O157:H7 infection was high for confirmed HIV/AIDS patients than for the non-confirmed HIV/AIDS patients. Estimated probability of risk of E. coli O157:H7 due to ingestion of water was 1.00 for 100 confirmed and non-confirmed HIV/AIDS patients. Risk due to meat and meat products was estimated at 0.27 and 0.20 and for vegetables at 0.21 and 0.15 per 100 confirmed and non-confirmed HIV/AIDS patients. The findings of this study predicted a possible link between E. coli O157:H7 isolated from drinking water, meat and meat products and vegetables and diarrhoeic conditions in both confirmed and non-confirmed HIV/AIDS patients, and concludes that confirmed HIV/AIDS patients can be at higher risk of contracting water and food borne E. coli O157:H7 than nonconfirmed HIV/AIDS patients. It is thus recommended that proper water treatment and food handling, maximum food and water safety and sanitation as well as personal body hygiene should be maintained, in order to prevent E. coli O157:H7 infections. Education initiatives and active surveillance of E. coli O157:H7 should be taken by all the stake-holders working directly or indirectly towards ensuring enduring sound public health.
- Full Text:
- Date Issued: 2008
- Authors: Abong'o, Benard Omondi
- Date: 2008
- Subjects: Foodborne diseases , Diarrhea , Escherichia coli , HIV infections , AIDS (Disease) , Bacterial diseases
- Language: English
- Type: Thesis , Doctoral , PhD (Microbiology)
- Identifier: vital:11263 , http://hdl.handle.net/10353/87 , Foodborne diseases , Diarrhea , Escherichia coli , HIV infections , AIDS (Disease) , Bacterial diseases
- Description: Water and food borne Escherichia coli O157:H7 could be one of the pathogens posing high health risk to patients suffering from Acquired Immunodeficiency Syndrome (AIDS) because of its incrimination in diarrhoea cases in AIDS patients. The present study, which was conducted between March 2005 and August 2006, investigated the prevalence of E. coli O157:H7 in water, meat and meat products and vegetables and its impact on diarrhoeic conditions of confirmed and non-confirmed HIV/AIDS patients in the Amathole District in the Eastern Cape Province of South Africa. The water samples used in the study were obtained from stand pipes supplying treated drinking water to communities residing in Fort Beaufort, Alice, Dimbaza and Mdantsane whereas borehole waters were sampled from Ngwenya and Kwasaki. The meat and meat products and vegetable samples were purchased from shops, butcheries, supermarkets and open air markets in Fort Beaufort, Alice and Mdantsane. The stool swabs used in the study were obtained from HIV/AIDS and outpatient clinics at Frere Hospital in East London. A total of 180 each of water, meat and meat products and vegetable samples and another 360 stool samples were analyzed for E. coli O157:H7. Presumptive E. coli O157 was isolated from the samples by culture-based methods and confirmed using Polymerase Chain Reaction techniques. Anti-biogram as well as risk assessment were also carried out using standard methods. The viable counts of presumptive E. coli O157 for water samples ranged between 3.3 × 104 and 1.71 × 105 CFU/ml, and between 1.8 × 104 and 5.04 × 106 CFU/g for meat and meat products, whereas those for vegetables ranged between 1.3 × 103 and 1.6 × 106 CFU/g. The counts of presumptive E. coli O157 for the water and vegetable samples were not significantly different whereas those for meat and meat products were found to be significantly different (P ≤ 0.05). The prevalence rates of presumptive E coli O157 in meat and meat products was 35.55 percent (64/180), and 25.55 percent (46/180) and 21.66 percent (39/180) for water and vegetables respectively. Prevalence of presumptive E. coli O157 in the stool samples of HIV/AIDS patients was 36.39 percent (131/360), of which 56.5 percent (74/131) and 43.5 percent (57/131) were from stools of confirmed and non-confirmed HIV/AIDS patients, respectively. Molecular analysis of representative presumptive E. coli O157 indicated that 10.29 percent (4/39) of vegetables; 14.81 percent (4/27) of water and 38.46 percent (5/13) of meat and meat products carried E. coli O157:H7. Also 36 percent (9/25) and 17.24 percent (5/29) of the stool samples were positive for E. coli O157:H7. Antimicrobial susceptibility profile revealed that all of the E. coli O157:H7 isolated from water, meat and meat products and vegetables as well as those isolated from stools of confirmed and non-confirmed HIV/AIDS patients were resistant (R) to gentamycin and erythromycin. However, 75 percent (20/27) of these isolates were resistant (R) to ampicillin and tetracycline whereas approximately 25 percent (6/27) were resistant (R) to nalidixic acid, ceftriaxone, and chloramphenicol. All the isolates (27/27) were susceptible (S) to amikacin. Probability of risk of E. coli O157:H7 infection was high for confirmed HIV/AIDS patients than for the non-confirmed HIV/AIDS patients. Estimated probability of risk of E. coli O157:H7 due to ingestion of water was 1.00 for 100 confirmed and non-confirmed HIV/AIDS patients. Risk due to meat and meat products was estimated at 0.27 and 0.20 and for vegetables at 0.21 and 0.15 per 100 confirmed and non-confirmed HIV/AIDS patients. The findings of this study predicted a possible link between E. coli O157:H7 isolated from drinking water, meat and meat products and vegetables and diarrhoeic conditions in both confirmed and non-confirmed HIV/AIDS patients, and concludes that confirmed HIV/AIDS patients can be at higher risk of contracting water and food borne E. coli O157:H7 than nonconfirmed HIV/AIDS patients. It is thus recommended that proper water treatment and food handling, maximum food and water safety and sanitation as well as personal body hygiene should be maintained, in order to prevent E. coli O157:H7 infections. Education initiatives and active surveillance of E. coli O157:H7 should be taken by all the stake-holders working directly or indirectly towards ensuring enduring sound public health.
- Full Text:
- Date Issued: 2008
Studies directed towards the synthesis of chromone carbaldehyde-derived HIV-1 protease inhibitors
- Authors: Molefe, Duduzile Mabel
- Date: 2008
- Subjects: Protease Inhibitors , HIV infections , HIV (Viruses) , AIDS (Disease) , Proteolytic enzymes , Heterocyclic compounds -- Derivatives , Chemical kinetics , Nuclear magnetic resonance spectroscopy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4526 , http://hdl.handle.net/10962/d1015542
- Description: A series of chromone-3-carbaldehydes have been prepared using Vilsmeier-Haack methodology while a corresponding series of chromone-2-carbaldeydes have been synthesized via the Kostanecki-Robinson reaction. Baylis-Hillman reactions have been conducted on both series of chromone carbaldehydes using three different catalysts, viz., 1,4-diazabicyclo(2.2.2]octane (DABCO), 1,8-diazabicyclo[5.4.0]undec- 7-ene (DBU) and 3-hydroxyquinuclidine (3HQ), and acrylonitrile, methyl acrylate and methyl vinyl ketone as the activated alkenes. These reactions have typically (but not always!) afforded both normal Baylis-Hillman and dimeric products. Attention has also been given to the use of 1-methyl-2-pyrrolidine (1-NMP), an ionic liquid, to replace normal organic solvents, and it has been found that, in the presence of DABCO, chromone-3-carbaldehydes afford the dimeric products alone. Reactions of chromone-3-carbaldehydes with methyl vinyl ketone have yielded unexpected, novel adducts, which appear to arise from preferential attack at C(2) in the chromone nucleus. Research on chromone-2-carbaldeydes under Baylis-Hillman conditions has also resulted in the formation of some interesting products instead of the expected Baylis-Hillman adducts. The Baylis-Hillman products have been explored as substrates for aza-Michael reactions using various amino derivatives including protected amino acids in the presence of the tetrabutylammonium bromide (TBAB) and the ionic liquid, 3-butyl-1- methylimidazoleboranetetrafluoride (BmimBF₄), as catalysts. The aza-Michael products have been targeted as truncated ritonavir analogues for investigation as potential HIV -1 protease inhibitors, and representative compounds have been subjected to enzyme inhibition assays to explore the extent and type of inhibition. Lineweaver-Burk and Dixon plots have indicated competitive inhibition in one case as well as non-competitive inhibition in another, and the inhibition constants (Ki) have been compared with that of the ritonavir. Computer modelling studies have also been conducted on selected chromonecontaining derivatives, using the ACCELRYS Cerius² platform. Interactive docking of the chromone-containing ligands into the HIV -1 protease receptor site, using the Ligandfit module, has indicated the importance of hydrogen-bonding interactions mediated by bridging water molecules situated in the receptor cavity. NMR spectroscopy has been used to elucidate complex and competing mechanistic pathways involved in the Baylis-Hillman reactions of selected 2-nitrobenzaldehydes with MVK in the presence of DABCO - reactions which afford the normal BaylisHillman product, the MVK dimer and syn- and anti-Baylis-Hillman type diadducts. The kinetic data confirm the concomitant operation of two pathways and reveal that, in the initial stage of the reaction, the product distribution is kinetically controlled, whereas in the latter stage, thermodynamic control results in the consumption of the normal Baylis-Hillman product and predominance of the anti-diadduct.
- Full Text:
- Date Issued: 2008
- Authors: Molefe, Duduzile Mabel
- Date: 2008
- Subjects: Protease Inhibitors , HIV infections , HIV (Viruses) , AIDS (Disease) , Proteolytic enzymes , Heterocyclic compounds -- Derivatives , Chemical kinetics , Nuclear magnetic resonance spectroscopy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4526 , http://hdl.handle.net/10962/d1015542
- Description: A series of chromone-3-carbaldehydes have been prepared using Vilsmeier-Haack methodology while a corresponding series of chromone-2-carbaldeydes have been synthesized via the Kostanecki-Robinson reaction. Baylis-Hillman reactions have been conducted on both series of chromone carbaldehydes using three different catalysts, viz., 1,4-diazabicyclo(2.2.2]octane (DABCO), 1,8-diazabicyclo[5.4.0]undec- 7-ene (DBU) and 3-hydroxyquinuclidine (3HQ), and acrylonitrile, methyl acrylate and methyl vinyl ketone as the activated alkenes. These reactions have typically (but not always!) afforded both normal Baylis-Hillman and dimeric products. Attention has also been given to the use of 1-methyl-2-pyrrolidine (1-NMP), an ionic liquid, to replace normal organic solvents, and it has been found that, in the presence of DABCO, chromone-3-carbaldehydes afford the dimeric products alone. Reactions of chromone-3-carbaldehydes with methyl vinyl ketone have yielded unexpected, novel adducts, which appear to arise from preferential attack at C(2) in the chromone nucleus. Research on chromone-2-carbaldeydes under Baylis-Hillman conditions has also resulted in the formation of some interesting products instead of the expected Baylis-Hillman adducts. The Baylis-Hillman products have been explored as substrates for aza-Michael reactions using various amino derivatives including protected amino acids in the presence of the tetrabutylammonium bromide (TBAB) and the ionic liquid, 3-butyl-1- methylimidazoleboranetetrafluoride (BmimBF₄), as catalysts. The aza-Michael products have been targeted as truncated ritonavir analogues for investigation as potential HIV -1 protease inhibitors, and representative compounds have been subjected to enzyme inhibition assays to explore the extent and type of inhibition. Lineweaver-Burk and Dixon plots have indicated competitive inhibition in one case as well as non-competitive inhibition in another, and the inhibition constants (Ki) have been compared with that of the ritonavir. Computer modelling studies have also been conducted on selected chromonecontaining derivatives, using the ACCELRYS Cerius² platform. Interactive docking of the chromone-containing ligands into the HIV -1 protease receptor site, using the Ligandfit module, has indicated the importance of hydrogen-bonding interactions mediated by bridging water molecules situated in the receptor cavity. NMR spectroscopy has been used to elucidate complex and competing mechanistic pathways involved in the Baylis-Hillman reactions of selected 2-nitrobenzaldehydes with MVK in the presence of DABCO - reactions which afford the normal BaylisHillman product, the MVK dimer and syn- and anti-Baylis-Hillman type diadducts. The kinetic data confirm the concomitant operation of two pathways and reveal that, in the initial stage of the reaction, the product distribution is kinetically controlled, whereas in the latter stage, thermodynamic control results in the consumption of the normal Baylis-Hillman product and predominance of the anti-diadduct.
- Full Text:
- Date Issued: 2008
The changing knowledge and expectations of public health nurses in a HIV/AIDS training programme for managers
- Authors: Harry, Karuna
- Date: 2001
- Subjects: HIV infections , HIV infections -- Nursing , HIV-positive persons -- Counseling of
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2986 , http://hdl.handle.net/10962/d1002495 , HIV infections , HIV infections -- Nursing , HIV-positive persons -- Counseling of
- Description: This research project examined the changing expectations and knowledge of Managers in the Department of Health who attended the Sexual Health Counselling Project offered by Rhodes University, East London, South Africa. These managers came from contexts in which the hierarchical medical model is firmly entrenched. The Sexual Health Counselling Project, drawing on theoretical principles from Narrative and other theories,presented a challenge to the standard management practices used by the managers. It also challenged how they dealt with clients. This research explored changes in expectations and knowledge prior to and during a two- week training course that the managers attended. A personal awareness and shift in knowledge occurred for many managers who examined their current practices. Some managers, who were firmly entrenched in the hierarchical model,found it difficult to change their ways of working.
- Full Text:
- Date Issued: 2001
- Authors: Harry, Karuna
- Date: 2001
- Subjects: HIV infections , HIV infections -- Nursing , HIV-positive persons -- Counseling of
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2986 , http://hdl.handle.net/10962/d1002495 , HIV infections , HIV infections -- Nursing , HIV-positive persons -- Counseling of
- Description: This research project examined the changing expectations and knowledge of Managers in the Department of Health who attended the Sexual Health Counselling Project offered by Rhodes University, East London, South Africa. These managers came from contexts in which the hierarchical medical model is firmly entrenched. The Sexual Health Counselling Project, drawing on theoretical principles from Narrative and other theories,presented a challenge to the standard management practices used by the managers. It also challenged how they dealt with clients. This research explored changes in expectations and knowledge prior to and during a two- week training course that the managers attended. A personal awareness and shift in knowledge occurred for many managers who examined their current practices. Some managers, who were firmly entrenched in the hierarchical model,found it difficult to change their ways of working.
- Full Text:
- Date Issued: 2001
HIV and metaphor: an imaginative response to illness
- Authors: Cardo, Julia Claire
- Date: 1998
- Subjects: HIV infections , Metaphor -- Psychological aspects , Imagery (Psychology) -- Therapeutic use
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2945 , http://hdl.handle.net/10962/d1002454 , HIV infections , Metaphor -- Psychological aspects , Imagery (Psychology) -- Therapeutic use
- Description: The medical model has been criticised for its failure to attend to individuals' experience of illness and the meaning they attribute to illness. HIV / AIDS has challenged its adequacy and brought the question of meaning in illness into sharp focus. This study aimed to understand what it means to live with HIV by exploring the fantasies, images and metaphors that make up the depth of such an experience. Phenomenology was deemed the appropriate approach, as it assigns epistemological significance to metaphor and ontological primacy to the lifeworld. An interview guide was fashioned from existing phenomenological literature and in-depth interviews were conducted with eight HIV -infected individuals. Five protocols were selected to constitute the study. In addition, an audio tape recording of one individual's metaphorical dialogue with HIV was obtained and transcribed. The three protocols with the richest content of imagery and metaphor were subjected to phenomenological explication. The remaining two protocols were used to support and clarify emergent meaning. A phenomenological explication of the data revealed a number of salient metaphors and themes. Upon being diagnosed with HIV, individuals were confronted with a socially and institutionally prescribed understanding of the disease; HIV as synonymous with AIDS and immediate death, HIV as sexual deviance, and HIV as myth. These metaphors influenced their conceptualisation and handling of HIV. Individual embodied metaphors included: embodying a heart of stone to live with HIV and perceiving HIV as a punishment from God, a demon from the Devil, a death sentence and a torture. Affectively, the experience of HIV was constituted as fear of physical disfigurement and exposure, anxiety, vulnerability, anger, betrayal, injustice and isolation. In a process of resolution and transformation, individuals imbibed positive metaphors with which to continue living with HIV. In order to cope with HIV, individuals seemed to negotiate a metaphorical space in which to dwell with their virus. This entailed establishing some form of dialogue with HIV or a Higher Power. This study revealed that metaphorical thinking about HIV /AIDS has a powerful impact on individuals' embodiment of their world. Metaphor is also an effective means Clf conveying and eliciting meaning in the experience of illness. Based upon these findings, it was suggested that metaphor be a prime focus for future research endeavours.
- Full Text:
- Date Issued: 1998
- Authors: Cardo, Julia Claire
- Date: 1998
- Subjects: HIV infections , Metaphor -- Psychological aspects , Imagery (Psychology) -- Therapeutic use
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2945 , http://hdl.handle.net/10962/d1002454 , HIV infections , Metaphor -- Psychological aspects , Imagery (Psychology) -- Therapeutic use
- Description: The medical model has been criticised for its failure to attend to individuals' experience of illness and the meaning they attribute to illness. HIV / AIDS has challenged its adequacy and brought the question of meaning in illness into sharp focus. This study aimed to understand what it means to live with HIV by exploring the fantasies, images and metaphors that make up the depth of such an experience. Phenomenology was deemed the appropriate approach, as it assigns epistemological significance to metaphor and ontological primacy to the lifeworld. An interview guide was fashioned from existing phenomenological literature and in-depth interviews were conducted with eight HIV -infected individuals. Five protocols were selected to constitute the study. In addition, an audio tape recording of one individual's metaphorical dialogue with HIV was obtained and transcribed. The three protocols with the richest content of imagery and metaphor were subjected to phenomenological explication. The remaining two protocols were used to support and clarify emergent meaning. A phenomenological explication of the data revealed a number of salient metaphors and themes. Upon being diagnosed with HIV, individuals were confronted with a socially and institutionally prescribed understanding of the disease; HIV as synonymous with AIDS and immediate death, HIV as sexual deviance, and HIV as myth. These metaphors influenced their conceptualisation and handling of HIV. Individual embodied metaphors included: embodying a heart of stone to live with HIV and perceiving HIV as a punishment from God, a demon from the Devil, a death sentence and a torture. Affectively, the experience of HIV was constituted as fear of physical disfigurement and exposure, anxiety, vulnerability, anger, betrayal, injustice and isolation. In a process of resolution and transformation, individuals imbibed positive metaphors with which to continue living with HIV. In order to cope with HIV, individuals seemed to negotiate a metaphorical space in which to dwell with their virus. This entailed establishing some form of dialogue with HIV or a Higher Power. This study revealed that metaphorical thinking about HIV /AIDS has a powerful impact on individuals' embodiment of their world. Metaphor is also an effective means Clf conveying and eliciting meaning in the experience of illness. Based upon these findings, it was suggested that metaphor be a prime focus for future research endeavours.
- Full Text:
- Date Issued: 1998
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