Hop as an anti-cancer drug target
- Vaaltyn, Michaelone Chantelle
- Authors: Vaaltyn, Michaelone Chantelle
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164704 , vital:41156 , doi:10.21504/10962/164704
- Description: Thesis (PhD)--Rhodes University, Biochemistry and Microbiology, 2020
- Full Text:
- Authors: Vaaltyn, Michaelone Chantelle
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164704 , vital:41156 , doi:10.21504/10962/164704
- Description: Thesis (PhD)--Rhodes University, Biochemistry and Microbiology, 2020
- Full Text:
Multiplexed Mass Spectrometry: Single, On-Bead, Detection Analysis Using MALDI-TOF MS
- Authors: Twala, Busisiwe Victoria
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164693 , vital:41155 , doi:10.21504/10962/164693
- Description: Thesis (PhD)--Rhodes University, Biochemistry and Microbiology, 2020
- Full Text:
- Authors: Twala, Busisiwe Victoria
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164693 , vital:41155 , doi:10.21504/10962/164693
- Description: Thesis (PhD)--Rhodes University, Biochemistry and Microbiology, 2020
- Full Text:
A narrative study of students’ and staff’s experiences of living with HIV and AIDS at Rhodes University
- Authors: Tsope, Lindiwe
- Date: 2021-04
- Subjects: AIDS (Disease) South Africa Makhanda , HIV infections South Africa Makhanda , College students Health and hygiene South Africa Makhanda , Universities and colleges South Africa Makhanda Employees Health and hygiene , Stigma (Social psychology) , AIDS (Disease) Social aspects South Africa Makhanda , HIV infections Social aspects South Africa Makhanda , AIDS (Disease) Psychological aspects , HIV infections Psychological aspects , Health counseling South Africa Makhanda , Discourse analysis, Narrative
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/176894 , vital:42769 , 10.21504/10962/176894
- Description: A narrative study of students’ and staff’s experiences of living with HIV and AIDS at Rhodes University Research on HIV and AIDS in university settings, especially research exploring the experience of living with the disease, has been minimal. As a response to the knowledge and research gaps, this thesis is a qualitative study involving students and staff living with HIV (LWH) and accessing treatment (ART) at the Rhodes University Health Care Centre. This study explored the personal and social symbolisms as well as meanings attached to living with HIV, through in-depth interviews with ten students and staff living with HIV, all purposively sampled and recruited through the Rhodes University Health Care Centre. Using social constructionism, symbolic interactionism and the theory of biographical disruption, the narratives revealed a positive and inspirational side of living with HIV and AIDS – especially emphasizing that PLWHA do not have to surrender to the deadly narrative of the disease. It became evident that stigma, both internal and external, largely influences illness narratives. Furthermore, the study revealed the social reconstruction of life narratives both in order to understand the illness in terms of past social experiences and to reaffirm the impression that life has a course and the self has a purpose. All participants found that accessing treatment from the Rhodes University Health Care Centre positively influenced their experiences of adherence and reconstruction of narratives. The study indicates that HIV-related interventions in place at the university need to pay more attention to the psychosocial needs of PLWH, involvement of PWLH, as well as keeping up with the continuously changing global HIV narrative. The study argues for more attention to in-depth experiences and personal narratives in HIV and AIDS, and PLWHA education at Rhodes University. , Thesis (PhD) -- Humanities, Department of Sociology, 2021
- Full Text:
- Authors: Tsope, Lindiwe
- Date: 2021-04
- Subjects: AIDS (Disease) South Africa Makhanda , HIV infections South Africa Makhanda , College students Health and hygiene South Africa Makhanda , Universities and colleges South Africa Makhanda Employees Health and hygiene , Stigma (Social psychology) , AIDS (Disease) Social aspects South Africa Makhanda , HIV infections Social aspects South Africa Makhanda , AIDS (Disease) Psychological aspects , HIV infections Psychological aspects , Health counseling South Africa Makhanda , Discourse analysis, Narrative
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/176894 , vital:42769 , 10.21504/10962/176894
- Description: A narrative study of students’ and staff’s experiences of living with HIV and AIDS at Rhodes University Research on HIV and AIDS in university settings, especially research exploring the experience of living with the disease, has been minimal. As a response to the knowledge and research gaps, this thesis is a qualitative study involving students and staff living with HIV (LWH) and accessing treatment (ART) at the Rhodes University Health Care Centre. This study explored the personal and social symbolisms as well as meanings attached to living with HIV, through in-depth interviews with ten students and staff living with HIV, all purposively sampled and recruited through the Rhodes University Health Care Centre. Using social constructionism, symbolic interactionism and the theory of biographical disruption, the narratives revealed a positive and inspirational side of living with HIV and AIDS – especially emphasizing that PLWHA do not have to surrender to the deadly narrative of the disease. It became evident that stigma, both internal and external, largely influences illness narratives. Furthermore, the study revealed the social reconstruction of life narratives both in order to understand the illness in terms of past social experiences and to reaffirm the impression that life has a course and the self has a purpose. All participants found that accessing treatment from the Rhodes University Health Care Centre positively influenced their experiences of adherence and reconstruction of narratives. The study indicates that HIV-related interventions in place at the university need to pay more attention to the psychosocial needs of PLWH, involvement of PWLH, as well as keeping up with the continuously changing global HIV narrative. The study argues for more attention to in-depth experiences and personal narratives in HIV and AIDS, and PLWHA education at Rhodes University. , Thesis (PhD) -- Humanities, Department of Sociology, 2021
- Full Text:
The development of an ionospheric storm-time index for the South African region
- Authors: Tshisaphungo, Mpho
- Date: 2021-04
- Subjects: Ionospheric storms -- South Africa , Global Positioning System , Neural networks (Computer science) , Regression analysis , Ionosondes , Auroral electrojet , Geomagnetic indexes , Magnetic storms -- South Africa
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178409 , vital:42937 , 10.21504/10962/178409
- Description: This thesis presents the development of a regional ionospheric storm-time model which forms the foundation of an index to provide a quick view of the ionospheric storm effects over South African mid-latitude region. The model is based on the foF2 measurements from four South African ionosonde stations. The data coverage for the model development over Grahamstown (33.3◦S, 26.5◦E), Hermanus (34.42◦S, 19.22◦E), Louisvale (28.50◦S, 21.20◦E), and Madimbo (22.39◦S, 30.88◦E) is 1996-2016, 2009-2016, 2000-2016, and 2000-2016 respectively. Data from the Global Positioning System (GPS) and radio occultation (RO) technique were used during validation. As the measure of either positive or negative storm effect, the variation of the critical frequency of the F2 layer (foF2) from the monthly median values (denoted as _foF2) is modeled. The modeling of _foF2 is based on only storm time data with the criteria of Dst 6 -50 nT and Kp > 4. The modeling methods used in the study were artificial neural network (ANN), linear regression (LR) and polynomial functions. The approach taken was to first test the modeling techniques on a single station before expanding the study to cover the regional aspect. The single station modeling was developed based on ionosonde data over Grahamstown. The inputs for the model which related to seasonal variation, diurnal variation, geomagnetic activity and solar activity were considered. For the geomagnetic activity, three indices namely; the symmetric disturbance in the horizontal component of the Earth’s magnetic field (SYM − H), the Auroral Electrojet (AE) index and local geomagnetic index A, were included as inputs. The performance of a single station model revealed that, of the three geomagnetic indices, SYM − H index has the largest contribution of 41% and 54% based on ANN and LR techniques respectively. The average correlation coefficients (R) for both ANN and LR models was 0.8, when validated during the selected storms falling within the period of model development. When validated using storms that fall outside the period of model development, the model gave R values of 0.6 and 0.5 for ANN and LR respectively. In addition, the GPS total electron content (TEC) derived measurements were used to estimate foF2 data. This is because there are more GPS receivers than ionosonde locations and the utilisation of this data increases the spatial coverage of the regional model. The estimation of foF2 from GPS TEC was done at GPS-ionosonde co-locations using polynomial functions. The average R values of 0.69 and 0.65 were obtained between actual and derived _foF2 over the co-locations and other GPS stations respectively. Validation of GPS TEC derived foF2 with RO data over regions out of ionospheric pierce points coverage with respect to ionosonde locations gave R greater than 0.9 for the selected storm period of 4-8 August 2011. The regional storm-time model was then developed based on the ANN technique using the four South African ionosonde stations. The maximum and minimum R values of 0.6 and 0.5 were obtained over ionosonde and GPS locations respectively. This model forms the basis towards the regional ionospheric storm-time index. , Thesis (PhD) -- Faculty of Science, Physics and Electronics, 2021
- Full Text:
- Authors: Tshisaphungo, Mpho
- Date: 2021-04
- Subjects: Ionospheric storms -- South Africa , Global Positioning System , Neural networks (Computer science) , Regression analysis , Ionosondes , Auroral electrojet , Geomagnetic indexes , Magnetic storms -- South Africa
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178409 , vital:42937 , 10.21504/10962/178409
- Description: This thesis presents the development of a regional ionospheric storm-time model which forms the foundation of an index to provide a quick view of the ionospheric storm effects over South African mid-latitude region. The model is based on the foF2 measurements from four South African ionosonde stations. The data coverage for the model development over Grahamstown (33.3◦S, 26.5◦E), Hermanus (34.42◦S, 19.22◦E), Louisvale (28.50◦S, 21.20◦E), and Madimbo (22.39◦S, 30.88◦E) is 1996-2016, 2009-2016, 2000-2016, and 2000-2016 respectively. Data from the Global Positioning System (GPS) and radio occultation (RO) technique were used during validation. As the measure of either positive or negative storm effect, the variation of the critical frequency of the F2 layer (foF2) from the monthly median values (denoted as _foF2) is modeled. The modeling of _foF2 is based on only storm time data with the criteria of Dst 6 -50 nT and Kp > 4. The modeling methods used in the study were artificial neural network (ANN), linear regression (LR) and polynomial functions. The approach taken was to first test the modeling techniques on a single station before expanding the study to cover the regional aspect. The single station modeling was developed based on ionosonde data over Grahamstown. The inputs for the model which related to seasonal variation, diurnal variation, geomagnetic activity and solar activity were considered. For the geomagnetic activity, three indices namely; the symmetric disturbance in the horizontal component of the Earth’s magnetic field (SYM − H), the Auroral Electrojet (AE) index and local geomagnetic index A, were included as inputs. The performance of a single station model revealed that, of the three geomagnetic indices, SYM − H index has the largest contribution of 41% and 54% based on ANN and LR techniques respectively. The average correlation coefficients (R) for both ANN and LR models was 0.8, when validated during the selected storms falling within the period of model development. When validated using storms that fall outside the period of model development, the model gave R values of 0.6 and 0.5 for ANN and LR respectively. In addition, the GPS total electron content (TEC) derived measurements were used to estimate foF2 data. This is because there are more GPS receivers than ionosonde locations and the utilisation of this data increases the spatial coverage of the regional model. The estimation of foF2 from GPS TEC was done at GPS-ionosonde co-locations using polynomial functions. The average R values of 0.69 and 0.65 were obtained between actual and derived _foF2 over the co-locations and other GPS stations respectively. Validation of GPS TEC derived foF2 with RO data over regions out of ionospheric pierce points coverage with respect to ionosonde locations gave R greater than 0.9 for the selected storm period of 4-8 August 2011. The regional storm-time model was then developed based on the ANN technique using the four South African ionosonde stations. The maximum and minimum R values of 0.6 and 0.5 were obtained over ionosonde and GPS locations respectively. This model forms the basis towards the regional ionospheric storm-time index. , Thesis (PhD) -- Faculty of Science, Physics and Electronics, 2021
- Full Text:
The effect of various substrate pretreatment methods on the enzymatic degradability of a Eucalyptus sp. – a potential feedstock for producing fermentable sugars
- Authors: Thoresen, Mariska
- Date: 2021-04
- Subjects: To be added
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178580 , vital:42952
- Description: Access restricted until April 2022. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Authors: Thoresen, Mariska
- Date: 2021-04
- Subjects: To be added
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178580 , vital:42952
- Description: Access restricted until April 2022. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
The Influence of Clientelism on the Informal Sector in Zimbabwe : a Case Study of Glen View 8 Complex, Harare
- Authors: Tandire, Justin
- Date: 2021-04
- Subjects: Patron and client -- Zimbabwe -- Harare , Informal sector (Economics) -- Zimbabwe -- Harare , Informal sector (Economics) -- Political aspects -- Zimbabwe -- Harare , Patronage, Political -- Zimbabwe -- Harare , ZANU-PF (Organization : Zimbabwe) , Zimbabwe -- Social conditions , Glen View Complex 8 (Zimbabwe)
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177986 , vital:42896 , 10.21504/10962/177986
- Description: This study focuses on the influence of clientelism in the informal sector of Zimbabwe in Glen View 8 (Complex). The study used the case of Glen view 8 (complex) in Harare Province. The study focused on political dynamics in the informal sector; livelihood strategies employed by informal sector operators; manifestation of “Big Men”, social networks in the informal sector; and different strategies employed by operators to overcome the problems of political manipulation, clientelism and patronage. It employs a qualitative research methodology to enable a nuanced comprehension of the clientelistic relationships that take place in the informal sector of Zimbabwe. Through in-depth interviews, focus group discussions, narratives and in-depth interviews with key informants, the study explored the clientelistic nature of the informal sector. The major findings of the study are that the informal sector in Zimbabwe is influenced by political patronage. It was established that patronage influences the informal sector in Zimbabwe in general and at Glen View Complex 8 in particular. Some of the operators revealed that patronage negatively affects their business as they are sometimes forced to attend political party meetings either at the complex or at ZANU-PF star rallies in town. The operators experience a plethora of problems such as lack of security, poor sanitation, stiff competition, poor infrastructure, lack of insurance and fire outbreaks. It has been revealed that most of the problems experienced at the complex are a result of the politicisation of the informal sector particularly by the ZANU-PF party. Operators at the complex have described the politicisation of the informal sector as a major drawback to their efforts of realising maximum benefits from their work. Therefore, the thrust of this thesis is premised on the de-politicisation of the informal sector as the starting point in the transformation of the activities of the operators. , Thesis (PhD) -- Faculty of Humanities, Sociology, 2021
- Full Text:
- Authors: Tandire, Justin
- Date: 2021-04
- Subjects: Patron and client -- Zimbabwe -- Harare , Informal sector (Economics) -- Zimbabwe -- Harare , Informal sector (Economics) -- Political aspects -- Zimbabwe -- Harare , Patronage, Political -- Zimbabwe -- Harare , ZANU-PF (Organization : Zimbabwe) , Zimbabwe -- Social conditions , Glen View Complex 8 (Zimbabwe)
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177986 , vital:42896 , 10.21504/10962/177986
- Description: This study focuses on the influence of clientelism in the informal sector of Zimbabwe in Glen View 8 (Complex). The study used the case of Glen view 8 (complex) in Harare Province. The study focused on political dynamics in the informal sector; livelihood strategies employed by informal sector operators; manifestation of “Big Men”, social networks in the informal sector; and different strategies employed by operators to overcome the problems of political manipulation, clientelism and patronage. It employs a qualitative research methodology to enable a nuanced comprehension of the clientelistic relationships that take place in the informal sector of Zimbabwe. Through in-depth interviews, focus group discussions, narratives and in-depth interviews with key informants, the study explored the clientelistic nature of the informal sector. The major findings of the study are that the informal sector in Zimbabwe is influenced by political patronage. It was established that patronage influences the informal sector in Zimbabwe in general and at Glen View Complex 8 in particular. Some of the operators revealed that patronage negatively affects their business as they are sometimes forced to attend political party meetings either at the complex or at ZANU-PF star rallies in town. The operators experience a plethora of problems such as lack of security, poor sanitation, stiff competition, poor infrastructure, lack of insurance and fire outbreaks. It has been revealed that most of the problems experienced at the complex are a result of the politicisation of the informal sector particularly by the ZANU-PF party. Operators at the complex have described the politicisation of the informal sector as a major drawback to their efforts of realising maximum benefits from their work. Therefore, the thrust of this thesis is premised on the de-politicisation of the informal sector as the starting point in the transformation of the activities of the operators. , Thesis (PhD) -- Faculty of Humanities, Sociology, 2021
- Full Text:
A novel Arf GTPase assay for antimalarial drug discovery
- Authors: Swart, Tarryn
- Date: 2021-04
- Subjects: To be added
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178558 , vital:42950
- Description: Access restricted until April 2022. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Authors: Swart, Tarryn
- Date: 2021-04
- Subjects: To be added
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178558 , vital:42950
- Description: Access restricted until April 2022. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
Investigating the factors that influence use of ICTs for citizen engagement in Malawi
- Authors: Sibande, Rachel Chavula
- Date: 2021-04
- Subjects: Information technology -- Malawi , Political participation -- Malawi , Mobile apps -- Malawi , UTAUT , Mzinda
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177019 , vital:42782 , 10.21504/10962/177019
- Description: Literature has suggested that Malawians are keen to participate. Malawian’s willingness to participate is evident as the country has recorded high voter turnouts during the elections in recent decades. However, literature also suggests that there is minimal citizen engagement in between elections. Elsewhere, Information and Communication Technologies (ICTs) have been used to enhance citizen engagement, but ICT led citizen engagement is still an emerging field and yet to be explored as an area of research particularly in Malawi. We thus sought to explore if the use of ICTs could improve citizen engagement with councils, councilors, and utility companies that provide water and electricity in Malawi. We developed and deployed an ICT platform called Mzinda which means My location in Malawi’s populous Chichewa language. The platform provided various channels for citizens and duty bearers to engage via SMS, USSD, web and a mobile application. We sought to understand the factors that influence citizen’s behavior intention to use an ICT platform to engage. We applied the modified UTAUT model by including Attitude and Self Efficacy social constructs that have among others been cited as limitations of the UTAUT model. We conducted factor loadings of six social constructs of the modified UTAUT model to validate content and reexamine the model in the context of citizen engagement using ICTs in Malawi. We found that, Attitude and Self Efficacy were not significant determinants of the Behaviour Intention for citizens to use the ICT platform. However, 75% of the Behaviour Intention was influenced by Perfomance Expectancy and Effort Expectancy as moderated by age and gender. Empirical evidence showed that responsiveness and actionability of councils and councillors had improved. We also learned that citizens believed that service delivery had improved and that they had more influence over councils, councillors, and the utility companies because of using the ICT platform. We conclude by noting that improvements in service delivery; enhanced responsiveness and actionability of councils, councillors and the utility companies were not necessarily as a result of the ICT platform alone; but a combination of ICTs and non-technology mechanisms of engaging the stakeholders through community campaigns, radio programs, print media engagement, community meetings and debates among others. It is evident that ICTs are not the panacea of all citizen engagement problems. This research can be useful to researchers and practitioners in the technology and citizen engagement domains. , Thesis (MSc) -- Faculty of Science, Department of Computer Science, 2021
- Full Text:
- Authors: Sibande, Rachel Chavula
- Date: 2021-04
- Subjects: Information technology -- Malawi , Political participation -- Malawi , Mobile apps -- Malawi , UTAUT , Mzinda
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177019 , vital:42782 , 10.21504/10962/177019
- Description: Literature has suggested that Malawians are keen to participate. Malawian’s willingness to participate is evident as the country has recorded high voter turnouts during the elections in recent decades. However, literature also suggests that there is minimal citizen engagement in between elections. Elsewhere, Information and Communication Technologies (ICTs) have been used to enhance citizen engagement, but ICT led citizen engagement is still an emerging field and yet to be explored as an area of research particularly in Malawi. We thus sought to explore if the use of ICTs could improve citizen engagement with councils, councilors, and utility companies that provide water and electricity in Malawi. We developed and deployed an ICT platform called Mzinda which means My location in Malawi’s populous Chichewa language. The platform provided various channels for citizens and duty bearers to engage via SMS, USSD, web and a mobile application. We sought to understand the factors that influence citizen’s behavior intention to use an ICT platform to engage. We applied the modified UTAUT model by including Attitude and Self Efficacy social constructs that have among others been cited as limitations of the UTAUT model. We conducted factor loadings of six social constructs of the modified UTAUT model to validate content and reexamine the model in the context of citizen engagement using ICTs in Malawi. We found that, Attitude and Self Efficacy were not significant determinants of the Behaviour Intention for citizens to use the ICT platform. However, 75% of the Behaviour Intention was influenced by Perfomance Expectancy and Effort Expectancy as moderated by age and gender. Empirical evidence showed that responsiveness and actionability of councils and councillors had improved. We also learned that citizens believed that service delivery had improved and that they had more influence over councils, councillors, and the utility companies because of using the ICT platform. We conclude by noting that improvements in service delivery; enhanced responsiveness and actionability of councils, councillors and the utility companies were not necessarily as a result of the ICT platform alone; but a combination of ICTs and non-technology mechanisms of engaging the stakeholders through community campaigns, radio programs, print media engagement, community meetings and debates among others. It is evident that ICTs are not the panacea of all citizen engagement problems. This research can be useful to researchers and practitioners in the technology and citizen engagement domains. , Thesis (MSc) -- Faculty of Science, Department of Computer Science, 2021
- Full Text:
CO2-induced woody thickening depends strongly on interacting abiotic and biotic factors
- Authors: Raubenheimer, Sarah Lynn
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164646 , vital:41151 , doi:10.21504/10962/164646
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Botany, 2020
- Full Text:
- Authors: Raubenheimer, Sarah Lynn
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164646 , vital:41151 , doi:10.21504/10962/164646
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Botany, 2020
- Full Text:
Application of In Vitro Release Testing (IVRT) and a dermatopharmacokinetic approach (tape stripping) for the assessment of Metronidazole topical formulations
- Rath, Seeprarani Prabirkumar
- Authors: Rath, Seeprarani Prabirkumar
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164617 , vital:41148 , doi:10.21504/10962/164617
- Description: Thesis (PhD)--Rhodes University, Faculty of Pharmacy, Pharmaceutics, 2020
- Full Text:
- Authors: Rath, Seeprarani Prabirkumar
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164617 , vital:41148 , doi:10.21504/10962/164617
- Description: Thesis (PhD)--Rhodes University, Faculty of Pharmacy, Pharmaceutics, 2020
- Full Text:
Accountability in the global health regime : a critical examination of the institutional policy and practice of the global fund to fight HIV/AIDS, tuberculosis and malaria partnership programme in Ghana
- Authors: Onokwai, John Chukwuemeka
- Date: 2021-04
- Subjects: World health , Global Fund to Fight AIDS, Tuberculosis, and Malaria -- Administration , Public-private sector cooperation -- Political aspects , Public-private sector cooperation -- Social aspects , Public-private sector cooperation -- Administration , Medical policy -- Ghana , Public health laws, International , Ghana -- Social conditions
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177099 , vital:42790 , 10.21504/10962/177099
- Description: The overarching objective of this thesis is to undertake a critical examination of the institutional accountability policy and practice of the Global Fund to Fight AIDS, Tuberculosis and Malaria (Global Fund) in the context of its partnership programme in Ghana. The Global Fund is a global public-private partnership (GPPP) in health engaged in public health policy processes worldwide. As a GPPP, the policy mandate that underpins its global response to fight the aforementioned diseases requires it to enter into partnerships with recipient countries to finance their national health policy responses and strategies to tackle these diseases. Situating accountability within the context of the shift from an international health to a global health regime, the study argues that the emergence of GPPPs in health and the formal policy mandate and decision-making powers they exercise have had knock-on consequences for understanding accountability in the global health regime. This is because while the understanding of accountability for public health policy processes in the international health regime revolved solely around state-based and state-led accountability processes, it is no longer so in current global health regime. Since these GPPPs are not states, they derive their understanding of accountability from the nature and character of their individual policy and practice arrangements. However, despite contestation around the Global Fund’s accountability in global health literature, this literature has little to say on the question of how the Global Fund itself (as a partnership organisation) understands accountability in policy and how this understanding informs its practice in specific settings of global health. Thus, this study contributes to literature on GPPPs’ accountability in global health by specifically exploring how the Global Fund understands accountability in policy and how this understanding informs its accountability in practice, in particular in relationship to its implications for country ownership of health policy in Ghana. Drawing on fieldwork undertaken in Ghana, and guided by a critical political economy approach, this study will demonstrate how: 1) the Global Fund’s institutional policy and practice arrangements undermine accountability to the government and to those affected by their activities; 2) the Global Fund’s practice of country ownership is reflective of conditional ownership despite the fact that the Global Fund claims to promote country ownership as a core principle of its accountability practice in aid recipient countries; and 3) the accountability policy and practice instruments of the Global Fund are not politically neutral, but are rather a function of relations of power. To improve the ability of Ghana (and other recipient countries) to own their developmental policies, a reordering of global economic relations is needed, with a renewed emphasis and focus on economic justice and human rights. Such a reordering will improve the material capabilities (control of and access to global centres of production, finance and technology) of aid recipient countries. This will empower Ghana (and other recipient countries) to play a more dominant, rather than a subsidiary role in how the global health landscape is organised and financed and in policy processes undertaken by global health policy institutions like the Global Fund. In this way, Ghana (and other developing countries) will be able to limit and mitigate the dominance and influence of powerful donors who shape the institutional policy and practice arrangements of global health policy institutions like the Global Fund. , Thesis (PhD) -- Faculty of Humanities, Department of Political and International Studies, 2021
- Full Text:
- Authors: Onokwai, John Chukwuemeka
- Date: 2021-04
- Subjects: World health , Global Fund to Fight AIDS, Tuberculosis, and Malaria -- Administration , Public-private sector cooperation -- Political aspects , Public-private sector cooperation -- Social aspects , Public-private sector cooperation -- Administration , Medical policy -- Ghana , Public health laws, International , Ghana -- Social conditions
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177099 , vital:42790 , 10.21504/10962/177099
- Description: The overarching objective of this thesis is to undertake a critical examination of the institutional accountability policy and practice of the Global Fund to Fight AIDS, Tuberculosis and Malaria (Global Fund) in the context of its partnership programme in Ghana. The Global Fund is a global public-private partnership (GPPP) in health engaged in public health policy processes worldwide. As a GPPP, the policy mandate that underpins its global response to fight the aforementioned diseases requires it to enter into partnerships with recipient countries to finance their national health policy responses and strategies to tackle these diseases. Situating accountability within the context of the shift from an international health to a global health regime, the study argues that the emergence of GPPPs in health and the formal policy mandate and decision-making powers they exercise have had knock-on consequences for understanding accountability in the global health regime. This is because while the understanding of accountability for public health policy processes in the international health regime revolved solely around state-based and state-led accountability processes, it is no longer so in current global health regime. Since these GPPPs are not states, they derive their understanding of accountability from the nature and character of their individual policy and practice arrangements. However, despite contestation around the Global Fund’s accountability in global health literature, this literature has little to say on the question of how the Global Fund itself (as a partnership organisation) understands accountability in policy and how this understanding informs its practice in specific settings of global health. Thus, this study contributes to literature on GPPPs’ accountability in global health by specifically exploring how the Global Fund understands accountability in policy and how this understanding informs its accountability in practice, in particular in relationship to its implications for country ownership of health policy in Ghana. Drawing on fieldwork undertaken in Ghana, and guided by a critical political economy approach, this study will demonstrate how: 1) the Global Fund’s institutional policy and practice arrangements undermine accountability to the government and to those affected by their activities; 2) the Global Fund’s practice of country ownership is reflective of conditional ownership despite the fact that the Global Fund claims to promote country ownership as a core principle of its accountability practice in aid recipient countries; and 3) the accountability policy and practice instruments of the Global Fund are not politically neutral, but are rather a function of relations of power. To improve the ability of Ghana (and other recipient countries) to own their developmental policies, a reordering of global economic relations is needed, with a renewed emphasis and focus on economic justice and human rights. Such a reordering will improve the material capabilities (control of and access to global centres of production, finance and technology) of aid recipient countries. This will empower Ghana (and other recipient countries) to play a more dominant, rather than a subsidiary role in how the global health landscape is organised and financed and in policy processes undertaken by global health policy institutions like the Global Fund. In this way, Ghana (and other developing countries) will be able to limit and mitigate the dominance and influence of powerful donors who shape the institutional policy and practice arrangements of global health policy institutions like the Global Fund. , Thesis (PhD) -- Faculty of Humanities, Department of Political and International Studies, 2021
- Full Text:
Development of paper-based aptasensors for detection of Plasmodium falciparum lactate dehydrogenase in malaria
- Ogunmolasuyi, Adewoyin Martin
- Authors: Ogunmolasuyi, Adewoyin Martin
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164601 , vital:41147 , doi:10.21504/10962/164601
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biotechnology Innovation Centre, 2020
- Full Text:
- Authors: Ogunmolasuyi, Adewoyin Martin
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164601 , vital:41147 , doi:10.21504/10962/164601
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biotechnology Innovation Centre, 2020
- Full Text:
Bioinformatic analysis of Aminoacyl tRNA Synthetases as potential antimalarial drug targets
- Authors: Nyamai, Dorothy Wavinya
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164579 , vital:41142 , doi:10.21504/10962/164579
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020
- Full Text:
- Authors: Nyamai, Dorothy Wavinya
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164579 , vital:41142 , doi:10.21504/10962/164579
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020
- Full Text:
A social realist study of employability development in engineering education
- Authors: Nudelman, Gabrielle Reeve
- Date: 2018
- Subjects: Critical realism , Electrical engineering -- Study and teaching (Higher) -- South Africa -- Cape Town , Employability , Career education -- South Africa -- Cape Town , School-to-work transition -- South Africa -- Cape Town
- Language: English
- Type: thesis , text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62884 , vital:28307
- Description: This qualitative case study of a course pairing offered to final-year electrical engineering students at the University of Cape Town in 2015 was undertaken in order to better understand the ways in which participation in undergraduate courses can prepare engineering students for the workplace. The course pairing consisted of New Venture Planning and Professional Communication Studies. While the former aimed to expose students to the knowledge relating to starting a new business, the latter focused on teaching students how to create written and oral texts to support such an endeavour. Using Roy Bhaskar’s critical realism as a theoretical underlabourer, the study develops understandings regarding the generative mechanisms at work during the two courses. In support of this, the study posits an understanding of employability that moves beyond the acquisition of discrete workplace skills. Rather, employability is conceptualised as discursive transformation, with students being deemed “work-ready” when they develop discursive identities as engineers. Data generation took place by means of focus group and individual interviews, ethnographic observation and documentary research. Margaret Archer’s social realist tools – in particular, analytical dualism and the morphogenetic framework were used to trace the students’ transformations over the course pairing. It was argued that those students who developed discursive identities of engineers were those who, in Archer’s terms, emerged as social actors at the end of the course pairing. Two characteristics of the courses were found to enable this transformation: those parts that promoted deepened understanding of what the role of “engineer” entailed and the parts that provided spaces for students to develop their own personal identities. The findings of the study indicated that discursive identities as engineers were more likely to be developed through the group work and spaces for reflection engendered by the courses than as a result of the formal curriculum. The implications of the research are that, while a focus on employability in engineering education is valid and productive, this needs to be supported by opportunities for authentic learning experiences which afford students the opportunity to engage in learning that promotes real-life application of knowledge. , Thesis (PhD)--Rhodes University, Faculty of Education, Education, 2018
- Full Text:
- Authors: Nudelman, Gabrielle Reeve
- Date: 2018
- Subjects: Critical realism , Electrical engineering -- Study and teaching (Higher) -- South Africa -- Cape Town , Employability , Career education -- South Africa -- Cape Town , School-to-work transition -- South Africa -- Cape Town
- Language: English
- Type: thesis , text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62884 , vital:28307
- Description: This qualitative case study of a course pairing offered to final-year electrical engineering students at the University of Cape Town in 2015 was undertaken in order to better understand the ways in which participation in undergraduate courses can prepare engineering students for the workplace. The course pairing consisted of New Venture Planning and Professional Communication Studies. While the former aimed to expose students to the knowledge relating to starting a new business, the latter focused on teaching students how to create written and oral texts to support such an endeavour. Using Roy Bhaskar’s critical realism as a theoretical underlabourer, the study develops understandings regarding the generative mechanisms at work during the two courses. In support of this, the study posits an understanding of employability that moves beyond the acquisition of discrete workplace skills. Rather, employability is conceptualised as discursive transformation, with students being deemed “work-ready” when they develop discursive identities as engineers. Data generation took place by means of focus group and individual interviews, ethnographic observation and documentary research. Margaret Archer’s social realist tools – in particular, analytical dualism and the morphogenetic framework were used to trace the students’ transformations over the course pairing. It was argued that those students who developed discursive identities of engineers were those who, in Archer’s terms, emerged as social actors at the end of the course pairing. Two characteristics of the courses were found to enable this transformation: those parts that promoted deepened understanding of what the role of “engineer” entailed and the parts that provided spaces for students to develop their own personal identities. The findings of the study indicated that discursive identities as engineers were more likely to be developed through the group work and spaces for reflection engendered by the courses than as a result of the formal curriculum. The implications of the research are that, while a focus on employability in engineering education is valid and productive, this needs to be supported by opportunities for authentic learning experiences which afford students the opportunity to engage in learning that promotes real-life application of knowledge. , Thesis (PhD)--Rhodes University, Faculty of Education, Education, 2018
- Full Text:
Formulation, development and assessment of devil’s claw loaded phyto-elastosomes in thermo-responsive hydrogels
- Authors: Ntemi, Pascal Vitalis
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164568 , vital:41139 , doi:10.21504/10962/164568
- Description: Thesis (PhD)--Rhodes University, Faculty of Pharmacy, Pharmaceutics, 2020
- Full Text:
- Authors: Ntemi, Pascal Vitalis
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164568 , vital:41139 , doi:10.21504/10962/164568
- Description: Thesis (PhD)--Rhodes University, Faculty of Pharmacy, Pharmaceutics, 2020
- Full Text:
The Trope of the Child: Rereading Trauma, Subjectivity and Embodiment in Contemporary Child-Centred African Narratives by Ahmadou Kourouma, Chris Abani, K. Sello Duiker and Yvonne Vera
- Authors: Njovane, Thandokazi
- Date: 2019
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164557 , vital:41134 , doi:10.21504/10962/164557
- Description: Thesis (PhD)--Rhodes University, Humanities, Literary Studies in English, 2019
- Full Text:
- Authors: Njovane, Thandokazi
- Date: 2019
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164557 , vital:41134 , doi:10.21504/10962/164557
- Description: Thesis (PhD)--Rhodes University, Humanities, Literary Studies in English, 2019
- Full Text:
A double-edged sword : the minimum wage and agrarian labour in the Eastern Cape, South Africa, 2003–2014
- Authors: Naidoo, Lalitha
- Date: 2021-04
- Subjects: Minimum wage -- South Africa -- Eastern Cape , Agricultural laborers -- South Africa -- Eastern Cape , Labor laws and legislation -- South Africa , Agricultural wages -- South Africa -- Eastern Cape , Agricultural laborers -- South Africa -- Eastern Cape -- Social conditions , Agricultural laborers -- Legal status, laws, etc. -- South Africa -- Eastern Cape , Agricultural laws and legislation -- South Africa -- Eastern Cape , Work environment -- South Africa -- Eastern Cape , Economics -- Sociological aspects , South Africa -- Politics and government -- 1994-
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177111 , vital:42791 , 10.21504/10962/177115
- Description: Statutory wage setting and the extension of labour laws to the South African agrarian labour market are path-breaking events that altered the unilateral power which agrarian employers had, under apartheid, to set low wages and harsh employment conditions. Yet, economic sociologists have shown little interest in the agrarian minimum wage in South Africa. Consequently, little to no sociological perspectives are available on the way in which statutory wages shape the setting of actual agrarian wages, employment conditions, labour relations and working and living conditions. At the same time, economic sociology’s neglect of agrarian minimum wages perpetuates the dominance of economics in minimum wage research, as well as the narrow cost-benefit analysis, and firm and employer-centric focus, deployed by both opponents of minimum wages in the neoclassical economics camp and supporters in the heterodox economics camp. The firm-centric focus also applies to the few labour relations scholars who focus on minimum wages. As a result, a wide body of empirical information is available and concentrated in the Global North, on low-waged, urban-based firms’ employment and labour relations strategies with the occasioning of minimum wage laws, with scant to non-existing information on rural-based workers’ experiences in the Global South, at least in the case of South Africa. This thesis addresses the lacuna in existing research, specifically by concentrating on agrarian workers’ narratives of the outcomes of minimum wages on actual wages and conditions, and experiences at the site of production and in the sphere of expanded social reproduction. The conceptual framework of the thesis is rooted in a critical realist meta-theory which directs inquiry towards the search for underlying causes of events with a sensitivity to the interaction of structure and agency, so as to develop explanations of events, which in turn encourage emancipatory thought and praxis. Within this framework, a political economy perspective of the agrarian minimum wage is charted, founded on an inter-disciplinary approach that incorporates economic sociology perspectives, which view markets as socio-political constructs, alongside a Marxist analysis of wages and the distinction between the value of labour and the value of labour power. Also relevant are segmentation labour market models where the focus is on segmentation in labour supply, demand and regulation, and institutional economics that highlights labour’s weak bargaining power in low-waged labour markets. Based on this analytical perspective, the South African agrarian minimum wage is seen as a necessary intervention stemming from post-apartheid uneven neoliberal restructuring processes, to address extremely low agrarian wages that pose threats to the ongoing generation of agrarian labour power. Low agrarian wages are located in unequal power relations in the workplace and are embedded in the totality of the low-waged agrarian labour market, composed of particular features in the supply-side of the labour market (the sphere of social reproduction of labour), the demand-side of the labour market (the site of production), and the forces of regulation (the labour relations regime). The thesis explores new ways of conceptualising minimum wages in the South African context, placing emphasis on the local agrarian labour market, and it highlights the agency of agrarian labour by revealing their struggles, working life and living conditions. In so doing, the research expands inquiry beyond economic “impact” at the level of the firm/employer to examine: (a) workers’ employment trends before and after the minimum wage was introduced, (b) the extent of changes in working and living conditions and labour relations, (c) the scope for workers in animating changes and their struggles and challenges, and (d) shifts in actual wages in relation to prescribed wage rates. Focussing on the aforementioned aspects represents an attempt in this thesis to build on themes, raised in heterodox economics studies, of minimum wages and their relationship to the social devaluation of low-waged work, inequalities in bargaining power, and justice. Based on a stratified sample of workers that included, among other variables, sex, geographical area and agricultural sub-sectors, original data was collected through 52 in-depth interviews, two focus group interviews (comprised of 10 workers), and 501 surveyed workers. The research did not find widespread job losses when minimum wages were introduced, as per neoclassical economics’ predictions. Nor did it find transitions from low- to high-road approaches in employment strategies and labour relations, as claimed by certain heterodox economists. Instead, the findings at the sites of production corroborate the uneven, mixed and contradictory findings of applied heterodox minimum wage studies on employment strategies, labour relations and wage settings. In this light, it is concluded that the agrarian minimum wage had layered outcomes for workers based on key findings, which include: (a) the minimum wage became the maximum wage as actual wages increased and clustered at the prescribed wage rate; (b) a level of gender wage parity close to the level of the prevailing prescribed minimum wage was found, but an overall gendered pattern to low-waged employment surfaced and manifested differently at sub-sector and enterprise levels; (c) though no changes were found in the way work was organised and how workers executed their tasks alongside no fundamental changes in the social relations of production, statutory minimum wages and limitations on working hours did reduce the hours of work and the existence of unpaid overtime work in certain sub-sectors such as livestock and dairy workplaces, through worker and employer initiatives (yet, at the same time, work intensification in compressed working hours appeared in the sample in other worksites, for example citrus workplaces); (d) authoritarian labour relations existed in varying depths and forms, based on sub-sector and enterprise characteristics, which shaped the propensity and scope for worker action; and (e) the layered outcomes of the agrarian minimum wage were felt at the site of social reproduction, where it brought a measure of relief for sampled workers; however, it was chronically inadequate to allow workers to meet their subsistence needs comprehensively. The research findings also highlight sub-sectoral complexities in changing employment and labour relations strategies from low- to high-road approaches in the agrarian sector. The layered outcomes of minimum wages for agrarian workers stems from the combined and uneven amalgamation of pre-existing and new conditions and relations consequent to neoliberalising processes in the agrarian political economy as well as the low minimum wage-setting. The thesis thus argues that the mixed outcomes reflect the layered character of the minimum wage as a conversion factor, which in turn equates to a layered notion of justice. This is because, on the one hand, the minimum wage ameliorates the plight of agrarian labour but, on the other hand, and given the bulwark of authoritarianism, pre-existing conditions and neoliberalising processes, the collective vulnerabilities in the agrarian labour market have expanded and may be intensifying. The agrarian minimum wage acts as a double-edged sword in contexts of mixed and layered outcomes for agrarian labour. A layered perspective of the conversion factor of a minimum wage exposes the possibilities and limitations of statutory wages as a conversion factor, based on context, and identifies the limits and possibilities for worker mobilisation and action. In the case of this research, the agrarian minimum wage deals in limited fashion with the value of labour power because of the initial and subsequent low settings; the minimum wage does not deal with class exploitation and the value of labour, although it sets the frame for instigating basic labour standards. The implications of a layered conversion potential of low minimum wage-settings are profound for conceptualising, theorising and researching the link between statutory wages and justice, with respect to the value of labour power and the value of labour. Future research on the minimum wage based on a Marxist reading of wages and located in real labour markets, strengthens heterodox approaches by deepening theories on the relationship between statutory wages, justice and production. , Thesis (PhD) -- Faculty of Humanities, Department of Sociology, 2021
- Full Text:
- Authors: Naidoo, Lalitha
- Date: 2021-04
- Subjects: Minimum wage -- South Africa -- Eastern Cape , Agricultural laborers -- South Africa -- Eastern Cape , Labor laws and legislation -- South Africa , Agricultural wages -- South Africa -- Eastern Cape , Agricultural laborers -- South Africa -- Eastern Cape -- Social conditions , Agricultural laborers -- Legal status, laws, etc. -- South Africa -- Eastern Cape , Agricultural laws and legislation -- South Africa -- Eastern Cape , Work environment -- South Africa -- Eastern Cape , Economics -- Sociological aspects , South Africa -- Politics and government -- 1994-
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177111 , vital:42791 , 10.21504/10962/177115
- Description: Statutory wage setting and the extension of labour laws to the South African agrarian labour market are path-breaking events that altered the unilateral power which agrarian employers had, under apartheid, to set low wages and harsh employment conditions. Yet, economic sociologists have shown little interest in the agrarian minimum wage in South Africa. Consequently, little to no sociological perspectives are available on the way in which statutory wages shape the setting of actual agrarian wages, employment conditions, labour relations and working and living conditions. At the same time, economic sociology’s neglect of agrarian minimum wages perpetuates the dominance of economics in minimum wage research, as well as the narrow cost-benefit analysis, and firm and employer-centric focus, deployed by both opponents of minimum wages in the neoclassical economics camp and supporters in the heterodox economics camp. The firm-centric focus also applies to the few labour relations scholars who focus on minimum wages. As a result, a wide body of empirical information is available and concentrated in the Global North, on low-waged, urban-based firms’ employment and labour relations strategies with the occasioning of minimum wage laws, with scant to non-existing information on rural-based workers’ experiences in the Global South, at least in the case of South Africa. This thesis addresses the lacuna in existing research, specifically by concentrating on agrarian workers’ narratives of the outcomes of minimum wages on actual wages and conditions, and experiences at the site of production and in the sphere of expanded social reproduction. The conceptual framework of the thesis is rooted in a critical realist meta-theory which directs inquiry towards the search for underlying causes of events with a sensitivity to the interaction of structure and agency, so as to develop explanations of events, which in turn encourage emancipatory thought and praxis. Within this framework, a political economy perspective of the agrarian minimum wage is charted, founded on an inter-disciplinary approach that incorporates economic sociology perspectives, which view markets as socio-political constructs, alongside a Marxist analysis of wages and the distinction between the value of labour and the value of labour power. Also relevant are segmentation labour market models where the focus is on segmentation in labour supply, demand and regulation, and institutional economics that highlights labour’s weak bargaining power in low-waged labour markets. Based on this analytical perspective, the South African agrarian minimum wage is seen as a necessary intervention stemming from post-apartheid uneven neoliberal restructuring processes, to address extremely low agrarian wages that pose threats to the ongoing generation of agrarian labour power. Low agrarian wages are located in unequal power relations in the workplace and are embedded in the totality of the low-waged agrarian labour market, composed of particular features in the supply-side of the labour market (the sphere of social reproduction of labour), the demand-side of the labour market (the site of production), and the forces of regulation (the labour relations regime). The thesis explores new ways of conceptualising minimum wages in the South African context, placing emphasis on the local agrarian labour market, and it highlights the agency of agrarian labour by revealing their struggles, working life and living conditions. In so doing, the research expands inquiry beyond economic “impact” at the level of the firm/employer to examine: (a) workers’ employment trends before and after the minimum wage was introduced, (b) the extent of changes in working and living conditions and labour relations, (c) the scope for workers in animating changes and their struggles and challenges, and (d) shifts in actual wages in relation to prescribed wage rates. Focussing on the aforementioned aspects represents an attempt in this thesis to build on themes, raised in heterodox economics studies, of minimum wages and their relationship to the social devaluation of low-waged work, inequalities in bargaining power, and justice. Based on a stratified sample of workers that included, among other variables, sex, geographical area and agricultural sub-sectors, original data was collected through 52 in-depth interviews, two focus group interviews (comprised of 10 workers), and 501 surveyed workers. The research did not find widespread job losses when minimum wages were introduced, as per neoclassical economics’ predictions. Nor did it find transitions from low- to high-road approaches in employment strategies and labour relations, as claimed by certain heterodox economists. Instead, the findings at the sites of production corroborate the uneven, mixed and contradictory findings of applied heterodox minimum wage studies on employment strategies, labour relations and wage settings. In this light, it is concluded that the agrarian minimum wage had layered outcomes for workers based on key findings, which include: (a) the minimum wage became the maximum wage as actual wages increased and clustered at the prescribed wage rate; (b) a level of gender wage parity close to the level of the prevailing prescribed minimum wage was found, but an overall gendered pattern to low-waged employment surfaced and manifested differently at sub-sector and enterprise levels; (c) though no changes were found in the way work was organised and how workers executed their tasks alongside no fundamental changes in the social relations of production, statutory minimum wages and limitations on working hours did reduce the hours of work and the existence of unpaid overtime work in certain sub-sectors such as livestock and dairy workplaces, through worker and employer initiatives (yet, at the same time, work intensification in compressed working hours appeared in the sample in other worksites, for example citrus workplaces); (d) authoritarian labour relations existed in varying depths and forms, based on sub-sector and enterprise characteristics, which shaped the propensity and scope for worker action; and (e) the layered outcomes of the agrarian minimum wage were felt at the site of social reproduction, where it brought a measure of relief for sampled workers; however, it was chronically inadequate to allow workers to meet their subsistence needs comprehensively. The research findings also highlight sub-sectoral complexities in changing employment and labour relations strategies from low- to high-road approaches in the agrarian sector. The layered outcomes of minimum wages for agrarian workers stems from the combined and uneven amalgamation of pre-existing and new conditions and relations consequent to neoliberalising processes in the agrarian political economy as well as the low minimum wage-setting. The thesis thus argues that the mixed outcomes reflect the layered character of the minimum wage as a conversion factor, which in turn equates to a layered notion of justice. This is because, on the one hand, the minimum wage ameliorates the plight of agrarian labour but, on the other hand, and given the bulwark of authoritarianism, pre-existing conditions and neoliberalising processes, the collective vulnerabilities in the agrarian labour market have expanded and may be intensifying. The agrarian minimum wage acts as a double-edged sword in contexts of mixed and layered outcomes for agrarian labour. A layered perspective of the conversion factor of a minimum wage exposes the possibilities and limitations of statutory wages as a conversion factor, based on context, and identifies the limits and possibilities for worker mobilisation and action. In the case of this research, the agrarian minimum wage deals in limited fashion with the value of labour power because of the initial and subsequent low settings; the minimum wage does not deal with class exploitation and the value of labour, although it sets the frame for instigating basic labour standards. The implications of a layered conversion potential of low minimum wage-settings are profound for conceptualising, theorising and researching the link between statutory wages and justice, with respect to the value of labour power and the value of labour. Future research on the minimum wage based on a Marxist reading of wages and located in real labour markets, strengthens heterodox approaches by deepening theories on the relationship between statutory wages, justice and production. , Thesis (PhD) -- Faculty of Humanities, Department of Sociology, 2021
- Full Text:
Indigenous knowledge of ecosystem services in rural communities of the Eastern Cape, South Africa
- Authors: Murata, Chenai
- Date: 2021-04
- Subjects: Ethnoscience -- South Africa -- Eastern Cape , Ecosystem management -- South Africa -- Eastern Cape , Nature -- Effect of human beings on -- South Africa -- Eastern Cape , Human ecology -- South Africa -- Eastern Cape , Human beings -- Effect of environment on -- South Africa -- Eastern Cape , South Africa -- Eastern Cape -- Rural conditions
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177929 , vital:42891 , 10.21504/10962/177929
- Description: This thesis is on indigenous knowledge of ecosystem services. The ecosystem service framework and its associated concepts are fairly young, having been introduced in the ecological discipline in the 1980s. The ecosystem service framework posits that the wellbeing of humans and their communities is dependent on services supplied by ecosystems. It emphasises that for the ecosystems to be able to supply the services, they need to be in a well-functioning state. This idea of well-functioning is predicated on the argument that the ecosystem service framework enjoins resource users to exercise responsible stewardship to prevent degradation and overharvesting. Moreover, the concept of dependence suggests that ecosystem services are of value to humans. The dominant means of measuring the value of ecosystem services has been the economic valuation method in which the contribution that each service makes to human wellbeing is quantified into monetary units. The framework disaggregates the services into four groups, namely provisioning, cultural, supporting and regulatory and seeks to all the pillars of human wellbeing including health, subsistence and spirituality into each of these groups. In doing all this, the framework significantly reconfigures the way we look at and present human-nature relations. This change has the potential to influence significant shifts in how ecological research and intervention programmes are conducted in the foreseeable future. However, the reality that the ecosystem service framework was formulated within, and is informed by the scientific epistemology begs the question: what do traditional rural communities who depend mainly on indigenous knowledge systems (IKS) for shaping and interoperating their relations with nature know of the ecosystem service concept? Rural communities are the ones who interact directly with nature on a day-to-day basis. This makes them a very critical component in the ecosystem service framework. Although South Africa has had studies in the ecosystem service theme, little has been done to attempt to investigate and document indigenous knowledge of ecosystem services that rural communities possess. By focusing only on scientific knowledge of ecosystem services, the South African literature does not do justice to the plural epistemologies of the ecosystem service users in the country. More importantly, the continued dearth of public information on indigenous knowledge of ecosystem services can potentially obstruct implementation of locally sensitive intervention programmes because nothing is known about how the local communities conceptualise the ecosystem service framework. All this presents a crucial gap in the South African research; one that unless effort is made to contribute towards filling it, our knowledge of how communities experience the ecosystem service framework in South Africa will remain skewed. This study set out to investigate and document indigenous knowledge of ecosystem services in order to contribute towards filling this gap. Indigenous knowledge system is an umbrella epistemic system that includes lay ecological knowledge (LEK), traditional ecological knowledge (TEK) and many other related organized systems of knowing. Although the thesis has a chapter on LEK, its primary focus was TEK because the thesis was interested in unravelling how aspects of tradition including taboos, customs, traditional rules and belief in ancestral forces influence local communities’ knowledge of some key aspects of the ecosystem service framework including knowledge of various ecosystem services, valuation of ecosystem services, management of ecosystem services and perceptions of the management practices. The decision to focus on TEK was based on the reasoning that rural communities of the Eastern Cape boast a strong reputation of being traditional, recognizing ancestral spirits, legends and taboos as critical tools of knowledge generation and transmission. Using both mixed methods in some chapters and the qualitative approach alone in others, the study collected data in five villages of Mgwalana, Mahlungulu, Colana, Gogela and Nozitshena located in the north eastern part of Eastern Cape Province of South Africa, a region formerly called Transkei. The data were collected not on ecosystem services; but on the local people’s knowledge of ecosystem services. Although literature was consulted, the study regarded respondents as the primary source of data, hence the findings and conclusions presented in this thesis are about what local people know about ecosystem services. The study uses critical realist theoretical lenses to interpret respondents’ reports. The lenses included the principle of the separation between ontology and epistemology, the iceberg metaphor of ontology, epistemological pluralism and the hermeneutic dimension. These lenses were used to make sense of both the knowledge system of local people and the things about which their knowledge was. As part of discussing the local people’s knowledge, the study sometimes refers to science. This is not because I expected them to demonstrate knowledge similar to science. Instead, it was a critical realist dialectical way of explaining what something is by demonstrating what it is not. The study made a couple of key findings that can potentially enhance the growth of the South African ecosystem service discipline. First, respondents demonstrated knowledge of ecosystem services by mentioning a range of them such as drinking water, medicinal plants, cultural plants and fuelwood and how they affect the wellbeing of humans. However, what they did not have good knowledge of is that nature services can be classified into the four groups of supporting, regulatory, cultural and provisioning. Among the four ecosystem services groups, respondents could identify two only; provisional and cultural. Second, local communities depend heavily on ecosystem services for their well-being. The services include fuelwood, construction timber, medicinal plants, wild fruits, wild fish, cultural services and thatch grass. Although they appreciate that ecosystem services have value to their wellbeing, local people found it difficult to represent the value in monetary units. The conditions that make it difficult for local people to perceive ecosystem services as commodities include the absence of well-defined property system, lack of a quantitative consumer tradition and absence of an economic conception of nature. Third, local people understand the need to keep ecosystems in a well-functioning state hence they implement several traditional practices to manage ecosystem services. These practices include taboos, designating certain resources as sacred, legends, customary law, as well as some secular practices including gelesha and stone terracing. However, it is not easy to understand how traditional management practices work because they are not empirically observable. Fourth, local people possess knowledge of the reality that if not well managed, ecosystems can undergo degradation and hence fail to supply the services needed for human wellbeing. However, they explain the causes of degradation in terms of changes observable at the empirical level and the invisible causal power of supernatural forces. The inclusion of natural forces in degradation explanations marks a departure from the scientific explanations that revolve around biophysical processes. Fifth, the use of traditional management practices such as taboos to management ecosystems is under threat at the local communities. The threat can be attributed to three groups of causes, namely changes in worldviews due to adoption of formal education and Christianity, institutional disharmony playing out between the state and local traditional leadership, and lifestyle changes. These challenges constrain the opportunity for local people to apply traditional management practices to prevent the degradation of ecosystems. The net implication of this is that it renders it difficult for researchers and policy makers to assess the effectiveness of traditional management practices because they are not being implemented in full. In light of all these findings, the thesis concludes that TEK is underlabouring for the ecosystem service framework in the sense that it is used by local communities to generate knowledge of ecological concepts and phenomena. This means that TEK does not exist for the sake of its own self. Drawing from this finding, the study proposes a framework of analysing TEK as an underlabourer for social-ecological triggers or issues. Nonetheless, there are few factors that can be sources of limitation to the study. These include the reality that it was difficult to access pure traditional knowledge because over the years the local communities have received many state-sponsored ecological intervention programmes and a possible personal bias given the reality that I grew up in a traditional household and my father was a key holder or TEK. , Thesis (PhD) -- Faculty of Science, Environmental Science, 2021 , Thesis chapter to be published in 'Green and Low-Carbon Economy'. Journal available: https://ojs.bonviewpress.com/index.php/GLCE/index
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- Authors: Murata, Chenai
- Date: 2021-04
- Subjects: Ethnoscience -- South Africa -- Eastern Cape , Ecosystem management -- South Africa -- Eastern Cape , Nature -- Effect of human beings on -- South Africa -- Eastern Cape , Human ecology -- South Africa -- Eastern Cape , Human beings -- Effect of environment on -- South Africa -- Eastern Cape , South Africa -- Eastern Cape -- Rural conditions
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/177929 , vital:42891 , 10.21504/10962/177929
- Description: This thesis is on indigenous knowledge of ecosystem services. The ecosystem service framework and its associated concepts are fairly young, having been introduced in the ecological discipline in the 1980s. The ecosystem service framework posits that the wellbeing of humans and their communities is dependent on services supplied by ecosystems. It emphasises that for the ecosystems to be able to supply the services, they need to be in a well-functioning state. This idea of well-functioning is predicated on the argument that the ecosystem service framework enjoins resource users to exercise responsible stewardship to prevent degradation and overharvesting. Moreover, the concept of dependence suggests that ecosystem services are of value to humans. The dominant means of measuring the value of ecosystem services has been the economic valuation method in which the contribution that each service makes to human wellbeing is quantified into monetary units. The framework disaggregates the services into four groups, namely provisioning, cultural, supporting and regulatory and seeks to all the pillars of human wellbeing including health, subsistence and spirituality into each of these groups. In doing all this, the framework significantly reconfigures the way we look at and present human-nature relations. This change has the potential to influence significant shifts in how ecological research and intervention programmes are conducted in the foreseeable future. However, the reality that the ecosystem service framework was formulated within, and is informed by the scientific epistemology begs the question: what do traditional rural communities who depend mainly on indigenous knowledge systems (IKS) for shaping and interoperating their relations with nature know of the ecosystem service concept? Rural communities are the ones who interact directly with nature on a day-to-day basis. This makes them a very critical component in the ecosystem service framework. Although South Africa has had studies in the ecosystem service theme, little has been done to attempt to investigate and document indigenous knowledge of ecosystem services that rural communities possess. By focusing only on scientific knowledge of ecosystem services, the South African literature does not do justice to the plural epistemologies of the ecosystem service users in the country. More importantly, the continued dearth of public information on indigenous knowledge of ecosystem services can potentially obstruct implementation of locally sensitive intervention programmes because nothing is known about how the local communities conceptualise the ecosystem service framework. All this presents a crucial gap in the South African research; one that unless effort is made to contribute towards filling it, our knowledge of how communities experience the ecosystem service framework in South Africa will remain skewed. This study set out to investigate and document indigenous knowledge of ecosystem services in order to contribute towards filling this gap. Indigenous knowledge system is an umbrella epistemic system that includes lay ecological knowledge (LEK), traditional ecological knowledge (TEK) and many other related organized systems of knowing. Although the thesis has a chapter on LEK, its primary focus was TEK because the thesis was interested in unravelling how aspects of tradition including taboos, customs, traditional rules and belief in ancestral forces influence local communities’ knowledge of some key aspects of the ecosystem service framework including knowledge of various ecosystem services, valuation of ecosystem services, management of ecosystem services and perceptions of the management practices. The decision to focus on TEK was based on the reasoning that rural communities of the Eastern Cape boast a strong reputation of being traditional, recognizing ancestral spirits, legends and taboos as critical tools of knowledge generation and transmission. Using both mixed methods in some chapters and the qualitative approach alone in others, the study collected data in five villages of Mgwalana, Mahlungulu, Colana, Gogela and Nozitshena located in the north eastern part of Eastern Cape Province of South Africa, a region formerly called Transkei. The data were collected not on ecosystem services; but on the local people’s knowledge of ecosystem services. Although literature was consulted, the study regarded respondents as the primary source of data, hence the findings and conclusions presented in this thesis are about what local people know about ecosystem services. The study uses critical realist theoretical lenses to interpret respondents’ reports. The lenses included the principle of the separation between ontology and epistemology, the iceberg metaphor of ontology, epistemological pluralism and the hermeneutic dimension. These lenses were used to make sense of both the knowledge system of local people and the things about which their knowledge was. As part of discussing the local people’s knowledge, the study sometimes refers to science. This is not because I expected them to demonstrate knowledge similar to science. Instead, it was a critical realist dialectical way of explaining what something is by demonstrating what it is not. The study made a couple of key findings that can potentially enhance the growth of the South African ecosystem service discipline. First, respondents demonstrated knowledge of ecosystem services by mentioning a range of them such as drinking water, medicinal plants, cultural plants and fuelwood and how they affect the wellbeing of humans. However, what they did not have good knowledge of is that nature services can be classified into the four groups of supporting, regulatory, cultural and provisioning. Among the four ecosystem services groups, respondents could identify two only; provisional and cultural. Second, local communities depend heavily on ecosystem services for their well-being. The services include fuelwood, construction timber, medicinal plants, wild fruits, wild fish, cultural services and thatch grass. Although they appreciate that ecosystem services have value to their wellbeing, local people found it difficult to represent the value in monetary units. The conditions that make it difficult for local people to perceive ecosystem services as commodities include the absence of well-defined property system, lack of a quantitative consumer tradition and absence of an economic conception of nature. Third, local people understand the need to keep ecosystems in a well-functioning state hence they implement several traditional practices to manage ecosystem services. These practices include taboos, designating certain resources as sacred, legends, customary law, as well as some secular practices including gelesha and stone terracing. However, it is not easy to understand how traditional management practices work because they are not empirically observable. Fourth, local people possess knowledge of the reality that if not well managed, ecosystems can undergo degradation and hence fail to supply the services needed for human wellbeing. However, they explain the causes of degradation in terms of changes observable at the empirical level and the invisible causal power of supernatural forces. The inclusion of natural forces in degradation explanations marks a departure from the scientific explanations that revolve around biophysical processes. Fifth, the use of traditional management practices such as taboos to management ecosystems is under threat at the local communities. The threat can be attributed to three groups of causes, namely changes in worldviews due to adoption of formal education and Christianity, institutional disharmony playing out between the state and local traditional leadership, and lifestyle changes. These challenges constrain the opportunity for local people to apply traditional management practices to prevent the degradation of ecosystems. The net implication of this is that it renders it difficult for researchers and policy makers to assess the effectiveness of traditional management practices because they are not being implemented in full. In light of all these findings, the thesis concludes that TEK is underlabouring for the ecosystem service framework in the sense that it is used by local communities to generate knowledge of ecological concepts and phenomena. This means that TEK does not exist for the sake of its own self. Drawing from this finding, the study proposes a framework of analysing TEK as an underlabourer for social-ecological triggers or issues. Nonetheless, there are few factors that can be sources of limitation to the study. These include the reality that it was difficult to access pure traditional knowledge because over the years the local communities have received many state-sponsored ecological intervention programmes and a possible personal bias given the reality that I grew up in a traditional household and my father was a key holder or TEK. , Thesis (PhD) -- Faculty of Science, Environmental Science, 2021 , Thesis chapter to be published in 'Green and Low-Carbon Economy'. Journal available: https://ojs.bonviewpress.com/index.php/GLCE/index
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Nutrient impacts on grasses and legumes growing in communal pasture soil in relation to mycorrhizal activity
- Authors: Mkile, Zolani
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164512 , vital:41125 , doi:10.21504/10962/164512
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020
- Full Text:
- Authors: Mkile, Zolani
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164512 , vital:41125 , doi:10.21504/10962/164512
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020
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An evaluation of synergistic interactions between feruloyl esterases and xylanases during the hydrolysis of various pre-treated agricultural residues
- Authors: Mkabayi, Lithalethu
- Date: 2021-04
- Subjects: Esterases , Xylanases , Hydrolysis , Agricultural wastes -- Recycling , Enzymes , Lignocellulose -- Biodegradation , Escherichia coli , Oligosaccharides , Hydroxycinnamic acids
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178224 , vital:42922 , 10.21504/10962/178224
- Description: Agricultural residues are readily available and inexpensive renewable resources that can be used as raw materials for the production of value-added chemicals. The application of enzymes to facilitate the degradation of agricultural residues has long been considered the most environmentally friendly strategy for converting this material into good quality value-added chemicals. However, agricultural residues are typically lignocellulosic in composition and recalcitrant to enzymatic hydrolysis. Due to this recalcitrant nature, the complete degradation of biomass residues requires the synergistic action of a broad range of enzymes. The development and optimisation of synergistic enzyme cocktails is an effective approach for achieving high hydrolysis efficiency of lignocellulosic biomass. The aim of the current study was to evaluate the synergistic interactions between two termite metagenome-derived feruloyl esterases (FAE6 and FAE5) and endo-xylanases for the production of hydroxycinnamic acids and xylo-oligosaccharides (XOS) from model substrates, and untreated and pre-treated agricultural residues. Firstly, the two fae genes were heterologously expressed in Escherichia coli, and the recombinant enzymes were purified to homogeneity. The biochemical properties of the purified recombinant FAEs and xylanases (XT6 and Xyn11) were then assessed to determine the factors which influenced their activities and to select suitable operating conditions for synergy studies. An optimal protein loading ratio of xylanases to FAEs required to maximise the release of both reducing sugar and ferulic acid (FA) was established using 0.5% (w/v) insoluble wheat arabinoxylan (a model substrate). The enzyme combination of 66% xylanase and 33% FAE (on a protein loading basis) produced the highest amounts of reducing sugars and FA. The enzyme combination of XT6 (GH10 xylanase) and FAE5 or FAE6 liberated the highest amount of FA while a combination of Xyn11 (GH11 xylanase) and FAE5 or FAE6 produced the highest reducing sugar content. The synergistic interactions which were established between the xylanases and FAEs were further investigated using agricultural residues (corn cobs, rice straw and sugarcane bagasse). The three substrates were subjected to hydrothermal and dilute acid pre-treatment prior to synergy studies. It is generally known that, during pre-treatment, many compounds can be produced which may influence enzymatic hydrolysis. The effects of these by-products were assessed and it was found that lignin and its degradation products were the most inhibitory to the FAEs. The optimised enzyme cocktail was then applied to 1% (w/v) of untreated and pre-treated substrates for the efficient production of XOS and hydroxycinnamic acids. A significant improvement in xylanase substrate degradation was observed, especially with the combination of 66% Xyn11 and 33% FAE6 which displayed an improvement in reducing sugars of approximately 1.9-fold and 3.4-fold for hydrothermal and acid pre-treated corn cobs (compared to when Xyn11 was used alone), respectively. The study demonstrated that pre-treatment substantially enhanced the enzymatic hydrolysis of corn cobs and rice straw. Analysis of the hydrolysate product profiles revealed that the optimised enzyme cocktail displayed great potential for releasing XOS with a low degree of polymerisation. In conclusion, this study provided significant insights into the mechanism of synergistic interactions between xylanases and metagenome-derived FAEs during the hydrolysis of various substrates. The study also demonstrated that optimised enzyme cocktails combined with low severity pre-treatment can facilitate the potential use of xylan-rich lignocellulosic biomass for the production of valuable products in the future. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Authors: Mkabayi, Lithalethu
- Date: 2021-04
- Subjects: Esterases , Xylanases , Hydrolysis , Agricultural wastes -- Recycling , Enzymes , Lignocellulose -- Biodegradation , Escherichia coli , Oligosaccharides , Hydroxycinnamic acids
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178224 , vital:42922 , 10.21504/10962/178224
- Description: Agricultural residues are readily available and inexpensive renewable resources that can be used as raw materials for the production of value-added chemicals. The application of enzymes to facilitate the degradation of agricultural residues has long been considered the most environmentally friendly strategy for converting this material into good quality value-added chemicals. However, agricultural residues are typically lignocellulosic in composition and recalcitrant to enzymatic hydrolysis. Due to this recalcitrant nature, the complete degradation of biomass residues requires the synergistic action of a broad range of enzymes. The development and optimisation of synergistic enzyme cocktails is an effective approach for achieving high hydrolysis efficiency of lignocellulosic biomass. The aim of the current study was to evaluate the synergistic interactions between two termite metagenome-derived feruloyl esterases (FAE6 and FAE5) and endo-xylanases for the production of hydroxycinnamic acids and xylo-oligosaccharides (XOS) from model substrates, and untreated and pre-treated agricultural residues. Firstly, the two fae genes were heterologously expressed in Escherichia coli, and the recombinant enzymes were purified to homogeneity. The biochemical properties of the purified recombinant FAEs and xylanases (XT6 and Xyn11) were then assessed to determine the factors which influenced their activities and to select suitable operating conditions for synergy studies. An optimal protein loading ratio of xylanases to FAEs required to maximise the release of both reducing sugar and ferulic acid (FA) was established using 0.5% (w/v) insoluble wheat arabinoxylan (a model substrate). The enzyme combination of 66% xylanase and 33% FAE (on a protein loading basis) produced the highest amounts of reducing sugars and FA. The enzyme combination of XT6 (GH10 xylanase) and FAE5 or FAE6 liberated the highest amount of FA while a combination of Xyn11 (GH11 xylanase) and FAE5 or FAE6 produced the highest reducing sugar content. The synergistic interactions which were established between the xylanases and FAEs were further investigated using agricultural residues (corn cobs, rice straw and sugarcane bagasse). The three substrates were subjected to hydrothermal and dilute acid pre-treatment prior to synergy studies. It is generally known that, during pre-treatment, many compounds can be produced which may influence enzymatic hydrolysis. The effects of these by-products were assessed and it was found that lignin and its degradation products were the most inhibitory to the FAEs. The optimised enzyme cocktail was then applied to 1% (w/v) of untreated and pre-treated substrates for the efficient production of XOS and hydroxycinnamic acids. A significant improvement in xylanase substrate degradation was observed, especially with the combination of 66% Xyn11 and 33% FAE6 which displayed an improvement in reducing sugars of approximately 1.9-fold and 3.4-fold for hydrothermal and acid pre-treated corn cobs (compared to when Xyn11 was used alone), respectively. The study demonstrated that pre-treatment substantially enhanced the enzymatic hydrolysis of corn cobs and rice straw. Analysis of the hydrolysate product profiles revealed that the optimised enzyme cocktail displayed great potential for releasing XOS with a low degree of polymerisation. In conclusion, this study provided significant insights into the mechanism of synergistic interactions between xylanases and metagenome-derived FAEs during the hydrolysis of various substrates. The study also demonstrated that optimised enzyme cocktails combined with low severity pre-treatment can facilitate the potential use of xylan-rich lignocellulosic biomass for the production of valuable products in the future. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text: