Storytelling through video game artworks – Twee kante van ’n storie
- Authors: Randall, Tasmin Tania
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425355 , vital:72232
- Description: This mini-thesis serves as a supporting document for my Master of Fine Art (MFA) exhibition, ‘Twee Kante van ‘n Storie’. The exhibition explores my culture and experiences as a ‘Coloured’ woman in Makhanda through storytelling. I use the term ‘Coloured’ with quotation marks to remain respectful to those who do not wish to reclaim the term. My minithesis analyses how video games as artworks can be a mode of storytelling and can encourage sociocultural awareness. In my research, I use storytelling as a tool and autoethnography as a methodology to both discuss and influence my practice. For my MFA installation, I have created a digital interactive website that uses the same language as a video game. Throughout my process, I have used two video games, That Dragon Cancer and Boet Fighter, as case studies, in order to help the building and creation of my autoethnographic art video game. My art video game explores my experiences of my culture and living in a small town. Through the creation of four fictionalised characters, which are loosely based on true life experiences and first-hand observations, I can reveal and unpack cultural experiences and biases that I have observed over the years. Each character in the game grapples with one of three prominent themes; stereotypes, ‘swagger’ and texturism. Furthermore, through using autoethnography as a methodology and the researcher as the phenomenon (Ellis, 2004: 45). This study contributes to the gap in ‘Coloured’ cultural diversities that exist outside the lens of the Western Cape experience. This is a perspective not commonly found within academia. , Thesis (MFA) -- Faculty of Humanities, Fine Art, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Randall, Tasmin Tania
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425355 , vital:72232
- Description: This mini-thesis serves as a supporting document for my Master of Fine Art (MFA) exhibition, ‘Twee Kante van ‘n Storie’. The exhibition explores my culture and experiences as a ‘Coloured’ woman in Makhanda through storytelling. I use the term ‘Coloured’ with quotation marks to remain respectful to those who do not wish to reclaim the term. My minithesis analyses how video games as artworks can be a mode of storytelling and can encourage sociocultural awareness. In my research, I use storytelling as a tool and autoethnography as a methodology to both discuss and influence my practice. For my MFA installation, I have created a digital interactive website that uses the same language as a video game. Throughout my process, I have used two video games, That Dragon Cancer and Boet Fighter, as case studies, in order to help the building and creation of my autoethnographic art video game. My art video game explores my experiences of my culture and living in a small town. Through the creation of four fictionalised characters, which are loosely based on true life experiences and first-hand observations, I can reveal and unpack cultural experiences and biases that I have observed over the years. Each character in the game grapples with one of three prominent themes; stereotypes, ‘swagger’ and texturism. Furthermore, through using autoethnography as a methodology and the researcher as the phenomenon (Ellis, 2004: 45). This study contributes to the gap in ‘Coloured’ cultural diversities that exist outside the lens of the Western Cape experience. This is a perspective not commonly found within academia. , Thesis (MFA) -- Faculty of Humanities, Fine Art, 2023
- Full Text:
- Date Issued: 2023-10-13
The activity and foraging patterns of the western rock elephant shrew (Elephantulus rupestris)
- Authors: Jackson, Emily Anne
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424402 , vital:72150
- Description: The increase, and prolonged periods, of high ambient temperatures are cause for concern for small mammals living in arid areas of Southern Africa. In this study, I aimed to determine the effect of high environmental temperatures on the activity, body temperature maintenance and foraging behaviour in a population of Western rock elephant shrew, Elephantulus rupestris, in the Eastern Cape, South Africa. I found that E. rupestris maintained stable body temperatures (Tb = 37.31 °C ± 0.64) over a range of environmental temperatures (Tair = 5° to 33.9 °C), indicating their ability to remain normothermic during the heat of the day. Under cool, mild and hot air temperatures, E. rupestris were predominantly active in the morning and night, being the least active during midday. This shift in activity time, known as polyphasic activity, is common amongst Macroscelidea species and is a mechanism for escaping thermal extremes. To determine the effect of temperature on foraging behaviour, I ran giving-up density (GUD) experiments on E. rupestris under a range of environmental conditions. GUD experiments yielded no conclusive results as animals did not show a significant preference for foraging under certain thermal conditions, and rather foraged under a variety of air temperatures. Overall, E. rupestris showed risk adverse behaviour, trading-off thermoregulation with foraging and activity for self-preservation during hot periods of the day. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Jackson, Emily Anne
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424402 , vital:72150
- Description: The increase, and prolonged periods, of high ambient temperatures are cause for concern for small mammals living in arid areas of Southern Africa. In this study, I aimed to determine the effect of high environmental temperatures on the activity, body temperature maintenance and foraging behaviour in a population of Western rock elephant shrew, Elephantulus rupestris, in the Eastern Cape, South Africa. I found that E. rupestris maintained stable body temperatures (Tb = 37.31 °C ± 0.64) over a range of environmental temperatures (Tair = 5° to 33.9 °C), indicating their ability to remain normothermic during the heat of the day. Under cool, mild and hot air temperatures, E. rupestris were predominantly active in the morning and night, being the least active during midday. This shift in activity time, known as polyphasic activity, is common amongst Macroscelidea species and is a mechanism for escaping thermal extremes. To determine the effect of temperature on foraging behaviour, I ran giving-up density (GUD) experiments on E. rupestris under a range of environmental conditions. GUD experiments yielded no conclusive results as animals did not show a significant preference for foraging under certain thermal conditions, and rather foraged under a variety of air temperatures. Overall, E. rupestris showed risk adverse behaviour, trading-off thermoregulation with foraging and activity for self-preservation during hot periods of the day. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
The adoption of international financial reporting standards and foreign direct investment inflows: the moderating effect of the institutional environment in Africa
- Authors: Simbi, Chipo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/419230 , vital:71627 , DOI 10.21504/10962/419230
- Description: Globalisation has created a need for an international accounting language to facilitate the smooth flow of trade across countries. In 2003, in an effort to establish a global financial reporting language, the International Accounting Standards Board (IASB) developed a single set of high-quality accounting principles known as the International Financial Reporting Standards (IFRS). Over the last decade, several African countries have adopted IFRS, and Africa has become the second-largest adopting continent after Europe. IFRS promotes improved quality of disclosure of accounting transactions, reduces information asymmetry between preparers and users of financial information, lowers the cost of investing, and breaks down information barriers to cross-border investment. Researchers suggest many benefits of IFRS adoption for macroeconomic indicators such as Foreign Direct Investment (FDI). The reduction in information acquisition and processing costs which translates into the reduction in investment costs, has been cited by most researchers. Researchers have argued, however, that the economic benefits of IFRS in Africa depend on the strength of the institutional environment. They also argue that the Western environment in which the IFRS was developed differs from the African environment. Thus, the universal approach of the IASB may not be appropriate due to the historical, social, economic and political context of African countries. The impact of the adoption of IFRS by African countries requires further examination, particularly as a weak institutional environment confronts many African countries. Three research questions are designed for this study; (1) Is there a significant change in FDI inflows for IFRS adopters in selected African countries after the adoption? (2) Is there a significant change in FDI inflows due to the institutional environment? (3) Does the institutional environment in IFRS-adopting countries moderate the effect of IFRS on FDI in selected African countries? The present study is underpinned by the new institutional theory, the information asymmetry theory, the eclectic theory and the signalling theory, each of which provide reasons why African countries have adopted IFRS. Nine hypotheses are developed, based on the research questions, and tested using the Systems General Method of Moments and the Difference-in-Difference method. The study uses data from 26 African countries, 15 adopting and 11 non-adopting countries, over the period 1996 - 2018. First, the study establishes that the adoption of IFRS positively and significantly affects FDI inflows into the selected sample of African countries. Second, the study concludes that legal enforcement, accounting and auditing standards enforcement, and language origin positively and significantly impact FDI inflows into these countries. Legal origin, however, has a positive but insignificant association with FDI inflows. Third, legal enforcement, historical ties, accounting and auditing enforcement and the quality of the institutional environment are found to moderate the effect of IFRS adoption on FDI inflows. These results indicate that IFRS is a crucial determinant of FDI inflows into African countries, but a supportive institutional environment is needed for African countries to attract FDI inflows after adoption. The results contribute to the accounting and finance literature on FDI into African countries, and may assist the investment community to assess the institutional risk associated with investing in IFRS adopting African countries. , Thesis (PhD) -- Faculty of Commerce, Accounting, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Simbi, Chipo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/419230 , vital:71627 , DOI 10.21504/10962/419230
- Description: Globalisation has created a need for an international accounting language to facilitate the smooth flow of trade across countries. In 2003, in an effort to establish a global financial reporting language, the International Accounting Standards Board (IASB) developed a single set of high-quality accounting principles known as the International Financial Reporting Standards (IFRS). Over the last decade, several African countries have adopted IFRS, and Africa has become the second-largest adopting continent after Europe. IFRS promotes improved quality of disclosure of accounting transactions, reduces information asymmetry between preparers and users of financial information, lowers the cost of investing, and breaks down information barriers to cross-border investment. Researchers suggest many benefits of IFRS adoption for macroeconomic indicators such as Foreign Direct Investment (FDI). The reduction in information acquisition and processing costs which translates into the reduction in investment costs, has been cited by most researchers. Researchers have argued, however, that the economic benefits of IFRS in Africa depend on the strength of the institutional environment. They also argue that the Western environment in which the IFRS was developed differs from the African environment. Thus, the universal approach of the IASB may not be appropriate due to the historical, social, economic and political context of African countries. The impact of the adoption of IFRS by African countries requires further examination, particularly as a weak institutional environment confronts many African countries. Three research questions are designed for this study; (1) Is there a significant change in FDI inflows for IFRS adopters in selected African countries after the adoption? (2) Is there a significant change in FDI inflows due to the institutional environment? (3) Does the institutional environment in IFRS-adopting countries moderate the effect of IFRS on FDI in selected African countries? The present study is underpinned by the new institutional theory, the information asymmetry theory, the eclectic theory and the signalling theory, each of which provide reasons why African countries have adopted IFRS. Nine hypotheses are developed, based on the research questions, and tested using the Systems General Method of Moments and the Difference-in-Difference method. The study uses data from 26 African countries, 15 adopting and 11 non-adopting countries, over the period 1996 - 2018. First, the study establishes that the adoption of IFRS positively and significantly affects FDI inflows into the selected sample of African countries. Second, the study concludes that legal enforcement, accounting and auditing standards enforcement, and language origin positively and significantly impact FDI inflows into these countries. Legal origin, however, has a positive but insignificant association with FDI inflows. Third, legal enforcement, historical ties, accounting and auditing enforcement and the quality of the institutional environment are found to moderate the effect of IFRS adoption on FDI inflows. These results indicate that IFRS is a crucial determinant of FDI inflows into African countries, but a supportive institutional environment is needed for African countries to attract FDI inflows after adoption. The results contribute to the accounting and finance literature on FDI into African countries, and may assist the investment community to assess the institutional risk associated with investing in IFRS adopting African countries. , Thesis (PhD) -- Faculty of Commerce, Accounting, 2023
- Full Text:
- Date Issued: 2023-10-13
The application of statistical classification to predict sovereign default
- Authors: Vele, Rendani
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424563 , vital:72164
- Description: When considering sovereign loans, it is imperative for a financial institution to have a good understanding of the sovereign they are transacting with. Defaults can occur if proper evaluation steps are not considered. To aid in the prediction of potential sovereign defaults, financial institutions, together with grading companies, quantify the risk associated with issuing a loan to a sovereign by developing sovereign default early warning systems (EWS). Various classification models are considered in this study to develop sovereign default EWS. These models are the binary logit, probit, Bayesian additive regression trees, and artificial neural networks. This study investigates the predictive performance of the various classification techniques. Sovereign information is not readily available, so missing data techniques are considered in order to counter the data availability issue. Sovereign defaults are rare, which results in an imbalance in the distribution of the binary dependent variable. To assess data sets with such characteristics, metrics for imbalanced data are considered for model performance comparison. From the findings, the Bayesian additive regression technique generated better results than the other techniques when considering a basic data analysis. Moreover when cross-validation was considered, the neural network technique performed best. In addition, regional models had better results than the global model when considering model predictive capability. The significance of this study is to develop sovereign default prediction models using various classification techniques focused on enhancing previous literature and analysis through the application of Bayesian additive regression trees. , Thesis (MSc) -- Faculty of Science, Statistics, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Vele, Rendani
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424563 , vital:72164
- Description: When considering sovereign loans, it is imperative for a financial institution to have a good understanding of the sovereign they are transacting with. Defaults can occur if proper evaluation steps are not considered. To aid in the prediction of potential sovereign defaults, financial institutions, together with grading companies, quantify the risk associated with issuing a loan to a sovereign by developing sovereign default early warning systems (EWS). Various classification models are considered in this study to develop sovereign default EWS. These models are the binary logit, probit, Bayesian additive regression trees, and artificial neural networks. This study investigates the predictive performance of the various classification techniques. Sovereign information is not readily available, so missing data techniques are considered in order to counter the data availability issue. Sovereign defaults are rare, which results in an imbalance in the distribution of the binary dependent variable. To assess data sets with such characteristics, metrics for imbalanced data are considered for model performance comparison. From the findings, the Bayesian additive regression technique generated better results than the other techniques when considering a basic data analysis. Moreover when cross-validation was considered, the neural network technique performed best. In addition, regional models had better results than the global model when considering model predictive capability. The significance of this study is to develop sovereign default prediction models using various classification techniques focused on enhancing previous literature and analysis through the application of Bayesian additive regression trees. , Thesis (MSc) -- Faculty of Science, Statistics, 2023
- Full Text:
- Date Issued: 2023-10-13
The complexities of transfer pricing methods and the role of advance pricing agreements and tax audits in addressing disputes
- Authors: Ndou, Wavhudi
- Date: 2023-10-13
- Subjects: Transfer pricing Taxation Law and legislation South Africa , Advance pricing agreement , Double taxation , Arms-length transactions , Tax auditing , Advance tax ruling , Organisation for Economic Co-operation and Development , United Nations , World Bank
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419631 , vital:71661
- Description: Base erosion and profit shifting is defined as the use of tax planning strategies by multinational enterprises, often through exploiting gaps and mismatches between the countries in which they operate (OECD, 2021: p. 1). Multinational enterprises exploit these gaps through the use of transfer pricing. Goods and services are exchanged between connected persons or associated enterprises at prices that do not reflect their arm’s length price, in order to shift profits from high tax to low tax jurisdictions. In terms of section 31 of the Income Tax Act, transactions between connected persons or associated enterprises must be reflected at their arm’s length price. Transfer pricing has become an issue due to the difficulties in determining an appropriate arm’s length price. Disputes arise between a taxpayer and a tax administration on the methods to use to determine an appropriate transfer price. The use of Advance Pricing Agreements prevents these disputes from arising and provides tax certainty on the treatment of transactions for both the taxpayer and the tax administration. While the OECD recommends the use of Advance Pricing Agreements as a method to prevent disputes from arising, the OECD also argued that if a country has the resources to conduct an audit, an Advance Pricing Agreement will not lead to increased revenue collection. The research therefore analyses the problems faced in determining an appropriate arm's length price and compares the role that Advance Pricing Agreements and audits play in addressing transfer pricing issues. The possible role of Advance Tax Rulings is also explored, but they are found not to be suitable, except for the most simple transactions. The research applies a legal interpretative, doctrinal research methodology and a qualitative research method. The data comprised of relevant South African tax legislation, OECD Guidelines, the World Bank Handbook, and the UN Manual, together with the writings of acknowledged experts in the field. The study establishes that a proper functioning audit system is crucial to increasing revenue collection once a country implements an Advance Pricing Agreement. The research therefore recommends the adoption of Advance Pricing Agreements in South Africa as a dispute prevention measure. , Thesis (MCom) -- Faculty of Commerce, Accounting, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Ndou, Wavhudi
- Date: 2023-10-13
- Subjects: Transfer pricing Taxation Law and legislation South Africa , Advance pricing agreement , Double taxation , Arms-length transactions , Tax auditing , Advance tax ruling , Organisation for Economic Co-operation and Development , United Nations , World Bank
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419631 , vital:71661
- Description: Base erosion and profit shifting is defined as the use of tax planning strategies by multinational enterprises, often through exploiting gaps and mismatches between the countries in which they operate (OECD, 2021: p. 1). Multinational enterprises exploit these gaps through the use of transfer pricing. Goods and services are exchanged between connected persons or associated enterprises at prices that do not reflect their arm’s length price, in order to shift profits from high tax to low tax jurisdictions. In terms of section 31 of the Income Tax Act, transactions between connected persons or associated enterprises must be reflected at their arm’s length price. Transfer pricing has become an issue due to the difficulties in determining an appropriate arm’s length price. Disputes arise between a taxpayer and a tax administration on the methods to use to determine an appropriate transfer price. The use of Advance Pricing Agreements prevents these disputes from arising and provides tax certainty on the treatment of transactions for both the taxpayer and the tax administration. While the OECD recommends the use of Advance Pricing Agreements as a method to prevent disputes from arising, the OECD also argued that if a country has the resources to conduct an audit, an Advance Pricing Agreement will not lead to increased revenue collection. The research therefore analyses the problems faced in determining an appropriate arm's length price and compares the role that Advance Pricing Agreements and audits play in addressing transfer pricing issues. The possible role of Advance Tax Rulings is also explored, but they are found not to be suitable, except for the most simple transactions. The research applies a legal interpretative, doctrinal research methodology and a qualitative research method. The data comprised of relevant South African tax legislation, OECD Guidelines, the World Bank Handbook, and the UN Manual, together with the writings of acknowledged experts in the field. The study establishes that a proper functioning audit system is crucial to increasing revenue collection once a country implements an Advance Pricing Agreement. The research therefore recommends the adoption of Advance Pricing Agreements in South Africa as a dispute prevention measure. , Thesis (MCom) -- Faculty of Commerce, Accounting, 2023
- Full Text:
- Date Issued: 2023-10-13
The contentious relationship between feminist scholarship and university sexual violence policies: 1980-2021
- Authors: Roberts, Lily May
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425267 , vital:72224
- Description: This thesis tracks the historically contentious relationship between feminist scholarship and university sexual violence policies. It examines the rise of the managerial university in South Africa, and the overlapping development of sexual violence policies through feminist activism from the early 1990s. Through an examination of the sexual violence policies of the University of Cape Town, the University of the Witwatersrand, Rhodes University, Stellenbosch University, and the University of the Western Cape, this thesis argues that these policies are aimed at ‘managing’ the process of reporting, rather than dismantling the broader structures – both within the university and society as a whole – that lead to and enable sexual violence. In this way, sexual violence policies are governed by a managerial discourse, limiting the possibility of addressing the dismantling of structures and discourses that perpetuate sexual violence, which, as I argue, is fundamentally opposed to the intellectual and political project of feminist scholarship from which these sexual violence policies initially emerged. However, this thesis also argues that feminist scholarship is not immune to the influence of managerialism, as discourses of efficiency and effectiveness have shaped Gender and Women’s Studies, where feminist scholarship is often located. I use the #RUReferenceList protests that drew on black feminist scholarship and came under managerial control as an example of the contentious relationship between managerialism and feminist activism. , Thesis (MA) -- Faculty of Humanities, History, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Roberts, Lily May
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425267 , vital:72224
- Description: This thesis tracks the historically contentious relationship between feminist scholarship and university sexual violence policies. It examines the rise of the managerial university in South Africa, and the overlapping development of sexual violence policies through feminist activism from the early 1990s. Through an examination of the sexual violence policies of the University of Cape Town, the University of the Witwatersrand, Rhodes University, Stellenbosch University, and the University of the Western Cape, this thesis argues that these policies are aimed at ‘managing’ the process of reporting, rather than dismantling the broader structures – both within the university and society as a whole – that lead to and enable sexual violence. In this way, sexual violence policies are governed by a managerial discourse, limiting the possibility of addressing the dismantling of structures and discourses that perpetuate sexual violence, which, as I argue, is fundamentally opposed to the intellectual and political project of feminist scholarship from which these sexual violence policies initially emerged. However, this thesis also argues that feminist scholarship is not immune to the influence of managerialism, as discourses of efficiency and effectiveness have shaped Gender and Women’s Studies, where feminist scholarship is often located. I use the #RUReferenceList protests that drew on black feminist scholarship and came under managerial control as an example of the contentious relationship between managerialism and feminist activism. , Thesis (MA) -- Faculty of Humanities, History, 2023
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- Date Issued: 2023-10-13
The development and op timisation of a Theiler’s murine encephalomyelitis virus antiviral assay
- Authors: Naidoo, Urisha Tirah
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424677 , vital:72174
- Description: Picornaviruses belong to the Picornaviridae family which are one of the largest and most diverse family of RNA viruses that cause a broad spectrum of infections in both humans and animals. These diseases range from severe infections such as poliomyelitis, meningitis, myocarditis to mild illnesses such as the common cold. Picornavirus outbreaks are a worldwide threat as they are continuously occurring. A recent outbreak of foot-and-mouth disease caused by a picornavirus occurred in South Africa, resulting in a temporary ban on the movement of cattle. Currently, the FDA has not approved any antiviral drugs against this virus, increasing the urgency for identifying effective antivirals. Picornaviruses have similar genomes and capsid organisation as such, those that are non-hazardous to humans can be used as a model system. A Theiler’s murine encephalomyelitis virus (TMEV) strain GDVII and Baby Hamster Kidney fibroblasts (BHK-21 cells) was used as a replication system to develop and optimise a medium-throughput antiviral screening assay. The TMEV GDVII replication system in BHK-21 cells was validated, and preliminary experiments were performed that were necessary for the development of the TMEV GDVII antiviral assay. This was achieved by conducting a CPE assay to visually monitor the onset and development of CPE induced by TMEV GDVII. Plaque assays accurately quantified the number of infectious virus particles required for calculating the MOI in downstream experiments. Lastly, indirect immunofluorescence and Western blot analysis detected the expression of viral proteins using previously generated antibodies against the TMEV GDVII VP1 capsid and 2C protein, thereby confirming infection in BHK-21 cells. The development of robust and reproducible assays is an essential component in antiviral drug discovery. Therefore, the confirmed replication system was then used as a foundation to develop a medium-throughput CPE-based TMEV GDVII antiviral assay whereby the parameters were optimised to produce one of high quality. Firstly, the quantitation of viral-induced CPE was examined and confirmed in a 96-well plate using resazurin as a cell viability indicator. Each parameter was tested at varying conditions, and the optimal was concluded as 2 % FBS in the assay media, a 15 000 cells/well seeding density, infecting the cells with TMEV GDVII at an MOI of 0.00625 and measuring resazurin at an endpoint of 72 hpi. Furthermore, the parameters were ii validated by calculating the Z’- factor, which consistently produced scores above 0.5, indicative of a reliable, robust, reproducible antiviral assay. Currently, there are no inhibitors against TMEV GDVII that have been reported or confirmed in cell lines, animal models or clinical trials. Therefore, once the optimal assay parameters were selected, it presented an opportunity to assess whether potential compounds, including itraconazole (ITZ) and dipyridamole (DIP), possessed antiviral activity that could firstly, be utilised as a control inhibitor when screening compounds against TMEV GDVII and secondly, contribute to research on this virus. Additionally, the previously produced anti-TMEV GDVII capsid antibody was shown to neutralise viral infection and was also included as a potential control. The sensitivity of the cells towards DMSO, a solution in which the compounds were solubilised, was first investigated. It was found that concentrations above 1 % are toxic to the cells; as such, the final DMSO concentrations were always kept below 1 % when screening compounds. Lastly, the generation of dose-response curves aided in the conclusion that the antibody was the most suitable control inhibitor as it displayed potent antiviral activity and no cytotoxicity towards the cells. In contrast, ITZ and DIP did not possess effective antiviral action and were toxic to cells at high concentrations. Finally, after all the components of the medium-throughput TMEV GDVII antiviral assay were identified, it was possible to screen 24 compounds from a coumarin and marine natural product library for cell cytotoxicity and antiviral activity. After generating dose-response curves, it was concluded that no compound effectively inhibited virus-induced CPE, and most were toxic to cells at relatively high concentrations. In conclusion, this is the first study that describes the development and optimisation of a robust medium-throughput CPE-based antiviral assay that has immense potential to screen other libraries of compounds for antiviral activity against TMEV GDVII. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Naidoo, Urisha Tirah
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424677 , vital:72174
- Description: Picornaviruses belong to the Picornaviridae family which are one of the largest and most diverse family of RNA viruses that cause a broad spectrum of infections in both humans and animals. These diseases range from severe infections such as poliomyelitis, meningitis, myocarditis to mild illnesses such as the common cold. Picornavirus outbreaks are a worldwide threat as they are continuously occurring. A recent outbreak of foot-and-mouth disease caused by a picornavirus occurred in South Africa, resulting in a temporary ban on the movement of cattle. Currently, the FDA has not approved any antiviral drugs against this virus, increasing the urgency for identifying effective antivirals. Picornaviruses have similar genomes and capsid organisation as such, those that are non-hazardous to humans can be used as a model system. A Theiler’s murine encephalomyelitis virus (TMEV) strain GDVII and Baby Hamster Kidney fibroblasts (BHK-21 cells) was used as a replication system to develop and optimise a medium-throughput antiviral screening assay. The TMEV GDVII replication system in BHK-21 cells was validated, and preliminary experiments were performed that were necessary for the development of the TMEV GDVII antiviral assay. This was achieved by conducting a CPE assay to visually monitor the onset and development of CPE induced by TMEV GDVII. Plaque assays accurately quantified the number of infectious virus particles required for calculating the MOI in downstream experiments. Lastly, indirect immunofluorescence and Western blot analysis detected the expression of viral proteins using previously generated antibodies against the TMEV GDVII VP1 capsid and 2C protein, thereby confirming infection in BHK-21 cells. The development of robust and reproducible assays is an essential component in antiviral drug discovery. Therefore, the confirmed replication system was then used as a foundation to develop a medium-throughput CPE-based TMEV GDVII antiviral assay whereby the parameters were optimised to produce one of high quality. Firstly, the quantitation of viral-induced CPE was examined and confirmed in a 96-well plate using resazurin as a cell viability indicator. Each parameter was tested at varying conditions, and the optimal was concluded as 2 % FBS in the assay media, a 15 000 cells/well seeding density, infecting the cells with TMEV GDVII at an MOI of 0.00625 and measuring resazurin at an endpoint of 72 hpi. Furthermore, the parameters were ii validated by calculating the Z’- factor, which consistently produced scores above 0.5, indicative of a reliable, robust, reproducible antiviral assay. Currently, there are no inhibitors against TMEV GDVII that have been reported or confirmed in cell lines, animal models or clinical trials. Therefore, once the optimal assay parameters were selected, it presented an opportunity to assess whether potential compounds, including itraconazole (ITZ) and dipyridamole (DIP), possessed antiviral activity that could firstly, be utilised as a control inhibitor when screening compounds against TMEV GDVII and secondly, contribute to research on this virus. Additionally, the previously produced anti-TMEV GDVII capsid antibody was shown to neutralise viral infection and was also included as a potential control. The sensitivity of the cells towards DMSO, a solution in which the compounds were solubilised, was first investigated. It was found that concentrations above 1 % are toxic to the cells; as such, the final DMSO concentrations were always kept below 1 % when screening compounds. Lastly, the generation of dose-response curves aided in the conclusion that the antibody was the most suitable control inhibitor as it displayed potent antiviral activity and no cytotoxicity towards the cells. In contrast, ITZ and DIP did not possess effective antiviral action and were toxic to cells at high concentrations. Finally, after all the components of the medium-throughput TMEV GDVII antiviral assay were identified, it was possible to screen 24 compounds from a coumarin and marine natural product library for cell cytotoxicity and antiviral activity. After generating dose-response curves, it was concluded that no compound effectively inhibited virus-induced CPE, and most were toxic to cells at relatively high concentrations. In conclusion, this is the first study that describes the development and optimisation of a robust medium-throughput CPE-based antiviral assay that has immense potential to screen other libraries of compounds for antiviral activity against TMEV GDVII. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
The development of a plate-based assay to detect the activation status of ARF1 GTPase in Plasmodium falciparum parasites
- Authors: Du Toit, Skye Carol
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424654 , vital:72172
- Description: The exponential rise in antimalarial drug resistance in the most infectious malaria species, Plasmodium falciparum, has emphasised the urgency to identify and validate novel drug targets that decrease parasite viability upon inhibition. In addition to several publications indicating that the regulation of human Arf1 GTPase activity (mediated by ArfGEFs and ArfGAPs) serves as a pertinent drug target for cancer research, the identification of Arf1 and its regulatory proteins in Plasmodium falciparum led to the question whether these protein homologs could be exploited as drug targets for anti-malarial drug therapies. To investigate this prospect, the establishment of a novel in vitro colorimetric ELISA-based assay was needed to be able to detect changes in the activation status of P. falciparum Arf1 (PfArf1) in parasite cultures exposed to potential Arf1 inhibitors. By exploiting the selective protein interaction that occurs between active GTP-bound Arf1 and its downstream effector, GGA3, an assay protocol was established that could be used to detect the activation status of purified, truncated PfArf1 obtained from E. coli and endogenous PfArf1 sourced from parasite lysates. The assay relies on the use of anti-Arf1 antibodies to detect the binding of active PfArf1 in the lysates of inhibitor-exposed cultured parasites to GST-GGA3 immobilised in glutathione-coated plates. The results from chemical validation experiments conducted using the novel assay developed in this study, using the known ArfGEF inhibitor brefeldin A (BFA) and ArfGAP inhibitors Chem1099 and Chem3050, yielded the anticipated results: decrease in active PfArf1 after parasite incubation with the ArfGEF inhibitor, and increased active PfArf1 after ArfGAP inhibition. The results confirmed PfArf1 as a potential anti-malarial drug target and encourages the further development of this assay format for the identification of subsequent inhibitors in library screening campaigns. Additional pilot experiments were conducted to further explore whether the assay could detect the activation status of human Arf1 using HeLa cell lysates and to provide further evidence that the assay could be exploited as a tool in the identification of Arf1 GTPase inhibitors with BFA and the known ArfGAP inhibitor, QS11. The results suggested that, while the assay can detect the increase in active cellular Arf1 due to the inhibition of human ArfGEF following BFA treatment, subsequent treatment with QS11 showed no evidence of a reduction in active human Arf1 due to ArfGAP inhibition. Further experimentation is required to investigate the ability the assay to confirm inhibition of human Arf1 deactivation by ArfGAP inhibitors and develop the assay as a useful tool to support cancer drug discovery, in addition to antimalarial drug discovery projects aimed at Arf1. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Du Toit, Skye Carol
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424654 , vital:72172
- Description: The exponential rise in antimalarial drug resistance in the most infectious malaria species, Plasmodium falciparum, has emphasised the urgency to identify and validate novel drug targets that decrease parasite viability upon inhibition. In addition to several publications indicating that the regulation of human Arf1 GTPase activity (mediated by ArfGEFs and ArfGAPs) serves as a pertinent drug target for cancer research, the identification of Arf1 and its regulatory proteins in Plasmodium falciparum led to the question whether these protein homologs could be exploited as drug targets for anti-malarial drug therapies. To investigate this prospect, the establishment of a novel in vitro colorimetric ELISA-based assay was needed to be able to detect changes in the activation status of P. falciparum Arf1 (PfArf1) in parasite cultures exposed to potential Arf1 inhibitors. By exploiting the selective protein interaction that occurs between active GTP-bound Arf1 and its downstream effector, GGA3, an assay protocol was established that could be used to detect the activation status of purified, truncated PfArf1 obtained from E. coli and endogenous PfArf1 sourced from parasite lysates. The assay relies on the use of anti-Arf1 antibodies to detect the binding of active PfArf1 in the lysates of inhibitor-exposed cultured parasites to GST-GGA3 immobilised in glutathione-coated plates. The results from chemical validation experiments conducted using the novel assay developed in this study, using the known ArfGEF inhibitor brefeldin A (BFA) and ArfGAP inhibitors Chem1099 and Chem3050, yielded the anticipated results: decrease in active PfArf1 after parasite incubation with the ArfGEF inhibitor, and increased active PfArf1 after ArfGAP inhibition. The results confirmed PfArf1 as a potential anti-malarial drug target and encourages the further development of this assay format for the identification of subsequent inhibitors in library screening campaigns. Additional pilot experiments were conducted to further explore whether the assay could detect the activation status of human Arf1 using HeLa cell lysates and to provide further evidence that the assay could be exploited as a tool in the identification of Arf1 GTPase inhibitors with BFA and the known ArfGAP inhibitor, QS11. The results suggested that, while the assay can detect the increase in active cellular Arf1 due to the inhibition of human ArfGEF following BFA treatment, subsequent treatment with QS11 showed no evidence of a reduction in active human Arf1 due to ArfGAP inhibition. Further experimentation is required to investigate the ability the assay to confirm inhibition of human Arf1 deactivation by ArfGAP inhibitors and develop the assay as a useful tool to support cancer drug discovery, in addition to antimalarial drug discovery projects aimed at Arf1. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
The dissipation of marital assets pending a divorce order in South Africa: a proposal for reform
- Mahlangu, Busisiwe Hlophane Maria
- Authors: Mahlangu, Busisiwe Hlophane Maria
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424130 , vital:72126
- Description: Dissipation of marital assets takes place where one spouses uses the marital assets with the intention to frustrate the equitable distribution at the dissolution of marriage. This kind of behaviour usually occurs when one spouse anticipates a divorce or when the final divorce order is pending. In this context then, this thesis aims to critically analyse the effectiveness of the legal remedies available to spouses who are negatively affected by the dissipation of marital assets in marriage in community of property and marriage out of community of property with accrual. In this thesis the question is asked: does the current law adequately protect one spouse from another spouse unfairly hiding or dissipating assets from their estate to prevent the equitable distribution of these assets to the other spouse on divorce? In order to answer this question, this thesis proceeds by examining each of the remedies in detail, looking specifically at the statutory remedies set out in the Matrimonial Property Act 88 of 1984, specifically those contained in ss 8, 15 and 20 of that Act. It also considers remedies found in the common law. Ultimately, the study finds that these remedies do not provide adequate protection to spouses affected by the dissipation of marital assets and looks at the particular reasons for this finding. The study then concludes by proposing reforms to provide better protection to affected spouses. These legal reforms include: amending the legislative framework, changing the determinative date of the accrual, legislating a requirement for full disclosure of assets in terms s 8(1) of the Matrimonial Property Act and, finally, advocating for a flexible interpretation of the requirements of s 20 of the Matrimonial Property Act and the common law anti-dissipation remedy. , Thesis (LLM) -- Faculty of Law, Law, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mahlangu, Busisiwe Hlophane Maria
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424130 , vital:72126
- Description: Dissipation of marital assets takes place where one spouses uses the marital assets with the intention to frustrate the equitable distribution at the dissolution of marriage. This kind of behaviour usually occurs when one spouse anticipates a divorce or when the final divorce order is pending. In this context then, this thesis aims to critically analyse the effectiveness of the legal remedies available to spouses who are negatively affected by the dissipation of marital assets in marriage in community of property and marriage out of community of property with accrual. In this thesis the question is asked: does the current law adequately protect one spouse from another spouse unfairly hiding or dissipating assets from their estate to prevent the equitable distribution of these assets to the other spouse on divorce? In order to answer this question, this thesis proceeds by examining each of the remedies in detail, looking specifically at the statutory remedies set out in the Matrimonial Property Act 88 of 1984, specifically those contained in ss 8, 15 and 20 of that Act. It also considers remedies found in the common law. Ultimately, the study finds that these remedies do not provide adequate protection to spouses affected by the dissipation of marital assets and looks at the particular reasons for this finding. The study then concludes by proposing reforms to provide better protection to affected spouses. These legal reforms include: amending the legislative framework, changing the determinative date of the accrual, legislating a requirement for full disclosure of assets in terms s 8(1) of the Matrimonial Property Act and, finally, advocating for a flexible interpretation of the requirements of s 20 of the Matrimonial Property Act and the common law anti-dissipation remedy. , Thesis (LLM) -- Faculty of Law, Law, 2023
- Full Text:
- Date Issued: 2023-10-13
The effect of piospheres on the ecology of insectivorous birds and their arthropod prey
- Authors: Balmer, Natasha Louise
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424380 , vital:72148
- Description: Desertification is the degradation of arid ecosystems that result in the loss of biodiversity. Piospheres are areas of local degradation around a central point due to overgrazing and increased herbivore presence. There is a paucity of information regarding the effect of localised degradation on arthropods and insectivorous birds. Both of these organisms play crucial roles in ecosystem functioning and stability and can be used as models to study ecosystem functioning. I investigated the effect of piospheres on arthropods and birds in the Eastern Cape province of South Africa. I found that the abundance and diversity of arthropods were significantly reduced inside the piospheres due to the lack of vegetation. Termites were a group specifically negatively impacted by piospheres, with a significant reduction in their presence inside the piosphere. The family composition of arthropods also changed inside and outside the piospheres, with Caelifera, Diptera and Formicidae being the most dominant groups. The diversity of birds was also significantly reduced due to the degradation inside the piospheres. Looking at insectivorous birds, I found that the reduction in both vegetation and arthropod prey availability resulted in non-random avoidance of piospheres. This shows that piospheres negatively impact both arthropods and birds. The results from my study are supported by other literature studying the effects of habitat degradation associated with desertification. Due to the similarities of degradation between piospheres and desertification I make the argument that piospheres can be studied as localised models of desertification. The decrease in vegetation and arthropod abundance and diversity was found to further impact the feeding success of insectivorous birds. Using piospheres as a model for desertification, I found that the foraging effort of birds is significantly reduced within a degraded area due to the lack of vegetation providing safety to arthropod prey species. In addition to this, the foraging efficiency of insectivorous birds is significantly reduced inside the piospheres due to the decreased arthropod abundance and diversity. This shows that inside the piospheres birds spend less time searching for insects and have fewer successful feeds. This has implications for desertification of arid environments where birds face hyperthermia. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Balmer, Natasha Louise
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424380 , vital:72148
- Description: Desertification is the degradation of arid ecosystems that result in the loss of biodiversity. Piospheres are areas of local degradation around a central point due to overgrazing and increased herbivore presence. There is a paucity of information regarding the effect of localised degradation on arthropods and insectivorous birds. Both of these organisms play crucial roles in ecosystem functioning and stability and can be used as models to study ecosystem functioning. I investigated the effect of piospheres on arthropods and birds in the Eastern Cape province of South Africa. I found that the abundance and diversity of arthropods were significantly reduced inside the piospheres due to the lack of vegetation. Termites were a group specifically negatively impacted by piospheres, with a significant reduction in their presence inside the piosphere. The family composition of arthropods also changed inside and outside the piospheres, with Caelifera, Diptera and Formicidae being the most dominant groups. The diversity of birds was also significantly reduced due to the degradation inside the piospheres. Looking at insectivorous birds, I found that the reduction in both vegetation and arthropod prey availability resulted in non-random avoidance of piospheres. This shows that piospheres negatively impact both arthropods and birds. The results from my study are supported by other literature studying the effects of habitat degradation associated with desertification. Due to the similarities of degradation between piospheres and desertification I make the argument that piospheres can be studied as localised models of desertification. The decrease in vegetation and arthropod abundance and diversity was found to further impact the feeding success of insectivorous birds. Using piospheres as a model for desertification, I found that the foraging effort of birds is significantly reduced within a degraded area due to the lack of vegetation providing safety to arthropod prey species. In addition to this, the foraging efficiency of insectivorous birds is significantly reduced inside the piospheres due to the decreased arthropod abundance and diversity. This shows that inside the piospheres birds spend less time searching for insects and have fewer successful feeds. This has implications for desertification of arid environments where birds face hyperthermia. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
The effectiveness of bank debt financing on the growth of small and medium enterprises (SMEs) in Namibia
- Paulus, Panduleni Hambeleleni
- Authors: Paulus, Panduleni Hambeleleni
- Date: 2023-10-13
- Subjects: Business enterprises Finance , Small business Namibia , Small and medium enterprises , Stakeholder management , Debt financing (Corporations)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419176 , vital:71622
- Description: Globally, SMEs are valued for their contribution to economic growth and development as well as for driving employment. To succeed, small firms require support especially in terms of obtaining funding and financial services that can enable them to meet day to day operational needs. Commercial banks play an important role for the financing of SMEs as small firms generally depend on bank loans to obtain external finance. However, despite the support and contribution, very little attention is given to the actual forms of finance used by small and medium-sized enterprises, the available finance made by lending institutions or investors and the relation between the use of the said debt finance and enterprise performance. Furthermore, several research studies carried out focusing on the effect of debt financing on performance of firms are inconsistent. Thus, this study sought to determine the effectiveness of bank debt finance on the growth of SMEs in Namibia. To achieve the objective of the study, it was important to have it rooted in the pragmatism paradigm; followed by both the deductive and inductive approaches. Interviews were conducted with the six SME owners and structured questionnaires were completed by the seven staff of the selected bank. The study used thematic analysis to analyze primary data from interviews by following three steps namely: reducing the data referred to as coding, analyzing data by creating patterns, and generating themes and drawing conclusion. Data from self-administered questionnaires was populated and textually analyzed aided by tables. The main findings of the study were that: (1) debt financing contributed to the growth and performance of SMEs as all firms under the study who made use of debt had experienced growth in terms of generated profits and acquired assets, (2) during the assessment and approving process, the bank looked at various lending factors and that collateral was not considered as prime to accessing funding, (3) the SMEs were not sufficiently funded and that there was no appropriate funding option for SMEs, (4) in terms of relationships, there was a lack of engagement and support between the bank and the SMEs. The support received from the bank was only in terms of lending. , Thesis (MBA) -- Faculty of Commerce, Rhodes Business School, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Paulus, Panduleni Hambeleleni
- Date: 2023-10-13
- Subjects: Business enterprises Finance , Small business Namibia , Small and medium enterprises , Stakeholder management , Debt financing (Corporations)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419176 , vital:71622
- Description: Globally, SMEs are valued for their contribution to economic growth and development as well as for driving employment. To succeed, small firms require support especially in terms of obtaining funding and financial services that can enable them to meet day to day operational needs. Commercial banks play an important role for the financing of SMEs as small firms generally depend on bank loans to obtain external finance. However, despite the support and contribution, very little attention is given to the actual forms of finance used by small and medium-sized enterprises, the available finance made by lending institutions or investors and the relation between the use of the said debt finance and enterprise performance. Furthermore, several research studies carried out focusing on the effect of debt financing on performance of firms are inconsistent. Thus, this study sought to determine the effectiveness of bank debt finance on the growth of SMEs in Namibia. To achieve the objective of the study, it was important to have it rooted in the pragmatism paradigm; followed by both the deductive and inductive approaches. Interviews were conducted with the six SME owners and structured questionnaires were completed by the seven staff of the selected bank. The study used thematic analysis to analyze primary data from interviews by following three steps namely: reducing the data referred to as coding, analyzing data by creating patterns, and generating themes and drawing conclusion. Data from self-administered questionnaires was populated and textually analyzed aided by tables. The main findings of the study were that: (1) debt financing contributed to the growth and performance of SMEs as all firms under the study who made use of debt had experienced growth in terms of generated profits and acquired assets, (2) during the assessment and approving process, the bank looked at various lending factors and that collateral was not considered as prime to accessing funding, (3) the SMEs were not sufficiently funded and that there was no appropriate funding option for SMEs, (4) in terms of relationships, there was a lack of engagement and support between the bank and the SMEs. The support received from the bank was only in terms of lending. , Thesis (MBA) -- Faculty of Commerce, Rhodes Business School, 2023
- Full Text:
- Date Issued: 2023-10-13
The effectiveness of prolonged exposure therapy for the treatment of PTSD and suicide ideation: a case study
- Authors: Mhlane, Zuziwe Gloria
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424997 , vital:72200
- Description: Post-Traumatic Stress Disorder (PTSD) is regarded as a public health burden in South Africa (SA) (Atwoli et al., 2013; Kagee, Bantjies & Saal 2017; Koenen et al.; 2017; Williams et al., 2007). According to McGowan & Kagee (2013), young adults in SA are a high-risk group of trauma. Exposure to trauma is also associated to comorbid conditions such as depression, anxiety suicidal ideation (SI) experienced by a large population (Bantjes, McGowan, Kagee & Steel, 2016). Prolonged Exposure (PE) is empirically supported and evaluated trauma therapy for PTSD (Foa, Hembree, & Rothbaum, 2007). However, there has been limited to no studies conducted to evaluate the effectiveness of PE in SA. Therefore, this study investigated whether PE is effective treatment for PTSD symptom reduction and suicide ideation in South Africa. Three participants were recruited from Focus on the Family SA (FOTF SA SA) through purposive sampling, and single-case experimental design (SCED) was utilised. PE was implemented online through zoom for 6 sessions on each client. At baseline, all the participants met the criteria for PTSD as per the Post-traumatic stress disorder symptom scale interview for DSM-5 (PSSI-5): Participant 1 (PSSI-5 - 63), Participant 2 (PSSI-5 - 53), Participant 3 (PSSI-5 - 63) and the clinical cut off score is 23. Furthermore, all participants were screened for suicide ideation (SI) using the Beck Suicide Inventory (BSI). All participants’ BSI score ranges were mild to moderate. Participants were also screened for depression using the Beck Depression Inventory second edition (BDI-II) and anxiety using the Beck Anxiety Inventory (BAI). The score ranges for both BDI-II and BAI were moderate to severe. The Clinical Outcomes in Routine Evaluation-Outcome Measure (CORE-OM) was also used to assess general psychological distress. The CORE-OM scores for all the participants for trauma, depression and anxiety were severe. However, the risk to self/SI for all the participants was minimal. During the intervention phase, all the participants scores showed a downtrend of PTSD symptomology, as per the Post-traumatic Stress Disorder Checklist for DSM-5 (PCL-5). The PCL-5 scores went down from PCL-5 score of 58 to final session PCL-5 score of 3. The clinical cut off score is 31-33. They were also screened using the BSI, BDI and BAI and the score ranges were mild. The CORE-OM scores for trauma, anxiety, depression, and risk to self/SI were also minimal. Overall, the preliminary result of this study supports the notion that adults with symptoms of PTSD and SI had improved at the end of a brief 6-session PE intervention. The comorbid depression also showed a downtrend. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mhlane, Zuziwe Gloria
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424997 , vital:72200
- Description: Post-Traumatic Stress Disorder (PTSD) is regarded as a public health burden in South Africa (SA) (Atwoli et al., 2013; Kagee, Bantjies & Saal 2017; Koenen et al.; 2017; Williams et al., 2007). According to McGowan & Kagee (2013), young adults in SA are a high-risk group of trauma. Exposure to trauma is also associated to comorbid conditions such as depression, anxiety suicidal ideation (SI) experienced by a large population (Bantjes, McGowan, Kagee & Steel, 2016). Prolonged Exposure (PE) is empirically supported and evaluated trauma therapy for PTSD (Foa, Hembree, & Rothbaum, 2007). However, there has been limited to no studies conducted to evaluate the effectiveness of PE in SA. Therefore, this study investigated whether PE is effective treatment for PTSD symptom reduction and suicide ideation in South Africa. Three participants were recruited from Focus on the Family SA (FOTF SA SA) through purposive sampling, and single-case experimental design (SCED) was utilised. PE was implemented online through zoom for 6 sessions on each client. At baseline, all the participants met the criteria for PTSD as per the Post-traumatic stress disorder symptom scale interview for DSM-5 (PSSI-5): Participant 1 (PSSI-5 - 63), Participant 2 (PSSI-5 - 53), Participant 3 (PSSI-5 - 63) and the clinical cut off score is 23. Furthermore, all participants were screened for suicide ideation (SI) using the Beck Suicide Inventory (BSI). All participants’ BSI score ranges were mild to moderate. Participants were also screened for depression using the Beck Depression Inventory second edition (BDI-II) and anxiety using the Beck Anxiety Inventory (BAI). The score ranges for both BDI-II and BAI were moderate to severe. The Clinical Outcomes in Routine Evaluation-Outcome Measure (CORE-OM) was also used to assess general psychological distress. The CORE-OM scores for all the participants for trauma, depression and anxiety were severe. However, the risk to self/SI for all the participants was minimal. During the intervention phase, all the participants scores showed a downtrend of PTSD symptomology, as per the Post-traumatic Stress Disorder Checklist for DSM-5 (PCL-5). The PCL-5 scores went down from PCL-5 score of 58 to final session PCL-5 score of 3. The clinical cut off score is 31-33. They were also screened using the BSI, BDI and BAI and the score ranges were mild. The CORE-OM scores for trauma, anxiety, depression, and risk to self/SI were also minimal. Overall, the preliminary result of this study supports the notion that adults with symptoms of PTSD and SI had improved at the end of a brief 6-session PE intervention. The comorbid depression also showed a downtrend. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
- Date Issued: 2023-10-13
The effectiveness of the Therapeutic Sandstory Method as a focused intervention with a child: a descriptive single case study
- Authors: Francis, Staci
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424973 , vital:72198
- Description: This study explores and describes a phenomenological case study, illustrating a postmodern constructionist orientation to sandtray work with a South African child. The Therapeutic Sandstory Method (TSM) involves the innovative integration of a variety of tenets when working with children– constructing sandworlds, storytelling, the reflection of stories and coherent therapeutic documents in therapy collectively facilitating the healing process. This novel method enables children to translate and communicate their own personal experiences and traumas into a concrete form through storytelling and metaphoric connections constructed in their sandworld. It is anticipated that the TSM is a brief yet feasible, child-centered therapeutic engagement designed to continue the therapeutic process outside of the therapy setting, making it ideally suited for children and adolescents from under-resourced communities. The results of the study revealed improved interpersonal functioning and emotional wellbeing after eight sessions. Contextualized narrative and social constructionist findings are discussed and recommendations made pertaining to future research and practice using TSM with children in therapy. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Francis, Staci
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424973 , vital:72198
- Description: This study explores and describes a phenomenological case study, illustrating a postmodern constructionist orientation to sandtray work with a South African child. The Therapeutic Sandstory Method (TSM) involves the innovative integration of a variety of tenets when working with children– constructing sandworlds, storytelling, the reflection of stories and coherent therapeutic documents in therapy collectively facilitating the healing process. This novel method enables children to translate and communicate their own personal experiences and traumas into a concrete form through storytelling and metaphoric connections constructed in their sandworld. It is anticipated that the TSM is a brief yet feasible, child-centered therapeutic engagement designed to continue the therapeutic process outside of the therapy setting, making it ideally suited for children and adolescents from under-resourced communities. The results of the study revealed improved interpersonal functioning and emotional wellbeing after eight sessions. Contextualized narrative and social constructionist findings are discussed and recommendations made pertaining to future research and practice using TSM with children in therapy. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
- Date Issued: 2023-10-13
The effects of different shift patterns on nurses’ sleep-wake behaviours in selected, private healthcare facilities
- Authors: Bell, Emma Catherine
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424219 , vital:72134
- Description: Nurses are required to work shifts to provide 24-hour care, in which they complete physically and mentally demanding tasks. The length and type of shifts, particularly night shifts interfere with the natural sleep-wake behaviours, leading to extended wakefulness and overall reduced sleep, and increase the likelihood of sleepiness during subsequent shifts. This can in turn affected various cognitive processes such attention, vigilance and alertness, which are necessary during the care process. Sleepiness as a result of working shifts has also been associated with an increased risk accidents and error during the delivery of care. Given the unique demands and ways in which workplaces are structured, each context arranges its shifts in unique ways and thus, in order to determine how to manage the effects of shift work, it is important to understand how it affects self-reported fatigue and sleep, of, in this case, nurses. While there has been extensive research on this in the global north, to date, there has been limited research aimed at examining the effects of shift work on nurses’ sleep-wake behaviours and fatigue in the South African context. Therefore, the aim of this study is to characterise shift arrangements in selected private facilities and explore its effects on private healthcare nurses. This study adopted a cross-sectional, survey design using an amended version of Standard Shiftwork Index. The questionnaire included demographic and shift details and explored the impact of the shift systems on nurse sleep-wake behaviours and disturbances and fatigue and workload. It was distributed among shift working nurses registered with the South African Nursing Council across three selected, private, healthcare facilities in the Eastern Cape, over a two-month period. The responses were analysed with descriptive and inferential statistics, with open-ended questions analysed using a thematic analysis. A total of 51 nurses completed the survey. Nurses worked 12-hour shifts which included night shifts and day shifts with fixed start and end times. Over time was commonly reported and generally, nurses reported having very little control over their shift arrangements. Overall, nurses slept less than what they reported they needed on duty days, with nurses working both day and night shifts reporting to sleep less than the globally recommended required sleep. This was compensated for by longer sleep durations during days off. The data collection revealed that three different shift arrangements were in use, including permanent day shifts, permanent night shifts and rotating shift work including nights, with permanent night nurses working significantly more consecutive shifts (seven) than the other two shift types and having significantly more days off (seven) as well. While there were no significant differences in self-reported sleep across the three shift types, permanent night nurses were found to have the shortest sleep. During days off, rotating nurse reported significantly longer sleep times compared to day shift workers which may point to the need to catch up from sleep debt. Rotating nurses experienced the greater total disturbances to their sleep than permanent day and permanent night shift nurses. While not statistically significant, it may point to the fact that rotating shift workers could not obtain regularly timed sleep (due to having to change their schedules) compared to permanent day and night nurses. Workload (physical, emotional, mental and time pressure) did not differ between the shifts (day or night) or the shift types, but did reflect a heavier workload, possibly due to the data collection occurring during the 5th wave of the COVID 19 pandemic. This study highlights that nurses in private healthcare facilities are working extended hours which were associated with reduced total sleep, irrespective of the nature of the shift, with rotating shift nurses experiencing some degree of greater disturbances to their sleep. The number, duration speed and direction of the shifts of rotating nurses needs to be explored further, whilst also exploring the influence of individual factors on sleep-wake behaviours of nurses. It may be beneficial for the healthcare facilities to implement fatigue management strategies to mitigate the negative impacts of shift work, given the impact that this may impact the delivery of care. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Bell, Emma Catherine
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424219 , vital:72134
- Description: Nurses are required to work shifts to provide 24-hour care, in which they complete physically and mentally demanding tasks. The length and type of shifts, particularly night shifts interfere with the natural sleep-wake behaviours, leading to extended wakefulness and overall reduced sleep, and increase the likelihood of sleepiness during subsequent shifts. This can in turn affected various cognitive processes such attention, vigilance and alertness, which are necessary during the care process. Sleepiness as a result of working shifts has also been associated with an increased risk accidents and error during the delivery of care. Given the unique demands and ways in which workplaces are structured, each context arranges its shifts in unique ways and thus, in order to determine how to manage the effects of shift work, it is important to understand how it affects self-reported fatigue and sleep, of, in this case, nurses. While there has been extensive research on this in the global north, to date, there has been limited research aimed at examining the effects of shift work on nurses’ sleep-wake behaviours and fatigue in the South African context. Therefore, the aim of this study is to characterise shift arrangements in selected private facilities and explore its effects on private healthcare nurses. This study adopted a cross-sectional, survey design using an amended version of Standard Shiftwork Index. The questionnaire included demographic and shift details and explored the impact of the shift systems on nurse sleep-wake behaviours and disturbances and fatigue and workload. It was distributed among shift working nurses registered with the South African Nursing Council across three selected, private, healthcare facilities in the Eastern Cape, over a two-month period. The responses were analysed with descriptive and inferential statistics, with open-ended questions analysed using a thematic analysis. A total of 51 nurses completed the survey. Nurses worked 12-hour shifts which included night shifts and day shifts with fixed start and end times. Over time was commonly reported and generally, nurses reported having very little control over their shift arrangements. Overall, nurses slept less than what they reported they needed on duty days, with nurses working both day and night shifts reporting to sleep less than the globally recommended required sleep. This was compensated for by longer sleep durations during days off. The data collection revealed that three different shift arrangements were in use, including permanent day shifts, permanent night shifts and rotating shift work including nights, with permanent night nurses working significantly more consecutive shifts (seven) than the other two shift types and having significantly more days off (seven) as well. While there were no significant differences in self-reported sleep across the three shift types, permanent night nurses were found to have the shortest sleep. During days off, rotating nurse reported significantly longer sleep times compared to day shift workers which may point to the need to catch up from sleep debt. Rotating nurses experienced the greater total disturbances to their sleep than permanent day and permanent night shift nurses. While not statistically significant, it may point to the fact that rotating shift workers could not obtain regularly timed sleep (due to having to change their schedules) compared to permanent day and night nurses. Workload (physical, emotional, mental and time pressure) did not differ between the shifts (day or night) or the shift types, but did reflect a heavier workload, possibly due to the data collection occurring during the 5th wave of the COVID 19 pandemic. This study highlights that nurses in private healthcare facilities are working extended hours which were associated with reduced total sleep, irrespective of the nature of the shift, with rotating shift nurses experiencing some degree of greater disturbances to their sleep. The number, duration speed and direction of the shifts of rotating nurses needs to be explored further, whilst also exploring the influence of individual factors on sleep-wake behaviours of nurses. It may be beneficial for the healthcare facilities to implement fatigue management strategies to mitigate the negative impacts of shift work, given the impact that this may impact the delivery of care. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
- Full Text:
- Date Issued: 2023-10-13
The effects of exploitation on the activity of Chrysoblephus laticeps in a thermally variable environment
- Authors: Mlotshwa, Nonhle Thubelihle
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424346 , vital:72145
- Description: Embargoed. Expected release date 2025. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mlotshwa, Nonhle Thubelihle
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424346 , vital:72145
- Description: Embargoed. Expected release date 2025. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
The effects of inclusive education policies and administrative circulars on small schools in Namibia
- Hamutumua, Teopolina Ndilina-Laudika
- Authors: Hamutumua, Teopolina Ndilina-Laudika
- Date: 2023-10-13
- Subjects: Inclusive education Namibia Kavango West , Education and state Namibia , Small schools Namibia Kavango West , Right to education Namibia Kavango West , Education Social aspects Namibia Kavango West , Business communication
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419737 , vital:71671
- Description: The achievement of the right to education for all children in Namibia is fundamentally dependent upon the quality of education provided to these learners irrespective of their social circumstances. Being a sparsely populated region, nearly half of the primary schools in the Kavango West region have fewer than 100 learners; thus, these small schools are regarded as not economically viable. The educational development in rural regions is shaped by limited access to quality, inclusive education and the persistence of low-quality primary education which has resulted in high repetition rates and dropouts. This research is a Critical Discourse Analysis of the education policies and regulations. As a case study, it unpacks Formal Education Circulars on staffing norms and on the closure of small schools, looking at their affordances/constraints on the provision of inclusive quality education for children schooling at small schools in Namibia. Interviews and document analysis were used to gather data. Critical Theory frames the process of ideological critique which, among other things, identifies inequalities and factors that limit human freedom and how such factors can be alleviated. The research asks the question: what are the explicit and implicit underlying realities experienced at one-man schools, which depict the varied situations in which these schools survive? Factors emerging from the study showed that small schools in Namibia are symbols of inequity and the exclusion of educationally marginalised children and their poor communities. Small schools are hardly able to sustain and draw in the resources required to meet the education needs of marginalised communities which fall under the multidimensional poverty index in Namibia. The study found that at the moment there are no deliberate efforts from the Ministry of Education to bring about the necessary changes to small schools, and therefore one-man schools have continued to operate as isolated sections of an inflexible system. It is my contention that deliberate efforts are still needed to improve small schools. Policy decisions that respond to the current challenges faced by one-man schools may be sufficient to bring about noteworthy changes in the operations of one-man schools and these changes could effectively impact the learners learning. The decision to drive change requires a multilayered approach that articulates a clear vision, which is systematically implemented to improve one-man schools in Namibia. , Thesis (MEd) -- Faculty of Education, Primary and Early Chilhood Education, 2023
- Full Text:
- Date Issued: 2023-10-13
The effects of inclusive education policies and administrative circulars on small schools in Namibia
- Authors: Hamutumua, Teopolina Ndilina-Laudika
- Date: 2023-10-13
- Subjects: Inclusive education Namibia Kavango West , Education and state Namibia , Small schools Namibia Kavango West , Right to education Namibia Kavango West , Education Social aspects Namibia Kavango West , Business communication
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419737 , vital:71671
- Description: The achievement of the right to education for all children in Namibia is fundamentally dependent upon the quality of education provided to these learners irrespective of their social circumstances. Being a sparsely populated region, nearly half of the primary schools in the Kavango West region have fewer than 100 learners; thus, these small schools are regarded as not economically viable. The educational development in rural regions is shaped by limited access to quality, inclusive education and the persistence of low-quality primary education which has resulted in high repetition rates and dropouts. This research is a Critical Discourse Analysis of the education policies and regulations. As a case study, it unpacks Formal Education Circulars on staffing norms and on the closure of small schools, looking at their affordances/constraints on the provision of inclusive quality education for children schooling at small schools in Namibia. Interviews and document analysis were used to gather data. Critical Theory frames the process of ideological critique which, among other things, identifies inequalities and factors that limit human freedom and how such factors can be alleviated. The research asks the question: what are the explicit and implicit underlying realities experienced at one-man schools, which depict the varied situations in which these schools survive? Factors emerging from the study showed that small schools in Namibia are symbols of inequity and the exclusion of educationally marginalised children and their poor communities. Small schools are hardly able to sustain and draw in the resources required to meet the education needs of marginalised communities which fall under the multidimensional poverty index in Namibia. The study found that at the moment there are no deliberate efforts from the Ministry of Education to bring about the necessary changes to small schools, and therefore one-man schools have continued to operate as isolated sections of an inflexible system. It is my contention that deliberate efforts are still needed to improve small schools. Policy decisions that respond to the current challenges faced by one-man schools may be sufficient to bring about noteworthy changes in the operations of one-man schools and these changes could effectively impact the learners learning. The decision to drive change requires a multilayered approach that articulates a clear vision, which is systematically implemented to improve one-man schools in Namibia. , Thesis (MEd) -- Faculty of Education, Primary and Early Chilhood Education, 2023
- Full Text:
- Date Issued: 2023-10-13
The effects of short-duration overnight kraaling on herbaceous vegetation and soils in mesic grassland
- Authors: Mgwali, Nompendulo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424721 , vital:72178
- Description: Land degradation is widespread in communal rangelands in the grassland biome of South Africa, and often attributed to overstocking and lack of coordinated management. Excessive pressure on the herbaceous component has contributed to the uncontrolled spread of opportunistic invasive alien woody species e.g. Acacia mearnsii in many degraded areas, resulting in significant loss of ecosystem service capacity, along with soil and land productivity. Short-duration overnight kraaling has been suggested as a tool for restoring degraded rangelands. Recent studies in semi-arid savannas and shrublands have reported increased grass cover, soil nutrients and palatability and concluded that short-duration kraaling is a low-cost and effective way of restoring degraded rangelands using livestock. However, the response of different plant functional types and communities to such intense livestock impact may vary depending on local context. This study used twelve paired kraal and control sites to investigate the effects of short-duration (7-24 days) overnight kraaling of livestock on herbaceous vegetation and soils in a mesic grassland. The study area is generally considered to be overgrazed but has considerable variation in grass composition and basal cover. Sites included relatively intact natural grassland and sites where wattle infestations had been cleared and where mostly bare ground remained. I tested the hypotheses that overnight kraaling would result in (1) increased basal cover due to introduction of grass seed and stimulation of germination through hoof action, (2) increased infiltration due to hoof action, and (3) increased soil nutrients and organic matter due to dung and urine deposition. I also hypothesized that factors such as a site’s initial grass cover, its slope, the occurrence and amount of rainfall before and during kraaling, and the kraaling intensity (number of livestock and duration of the kraaling event) would influence the magnitude and direction of the kraaling effect. The effect of kraaling on vegetation was strongly dependent on initial condition. Kraaling increased basal cover of grasses when sites had low initial basal cover, but decreased basal cover if initial values were over 50%. Infiltration increased if kraaling took place during or after rain but decreased if kraaling took place when soils were dry. Kraaling increased soil P and K. In mesic grasslands, short-duration overnight kraaling is promising as a tool for rehabilitating degraded sites but should be avoided where the grass sward is relatively intact. I recommend that the suitability of kraaling be further evaluated per vegetation type and local context. , Thesis (MSc) -- Faculty of Science, Botany, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mgwali, Nompendulo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424721 , vital:72178
- Description: Land degradation is widespread in communal rangelands in the grassland biome of South Africa, and often attributed to overstocking and lack of coordinated management. Excessive pressure on the herbaceous component has contributed to the uncontrolled spread of opportunistic invasive alien woody species e.g. Acacia mearnsii in many degraded areas, resulting in significant loss of ecosystem service capacity, along with soil and land productivity. Short-duration overnight kraaling has been suggested as a tool for restoring degraded rangelands. Recent studies in semi-arid savannas and shrublands have reported increased grass cover, soil nutrients and palatability and concluded that short-duration kraaling is a low-cost and effective way of restoring degraded rangelands using livestock. However, the response of different plant functional types and communities to such intense livestock impact may vary depending on local context. This study used twelve paired kraal and control sites to investigate the effects of short-duration (7-24 days) overnight kraaling of livestock on herbaceous vegetation and soils in a mesic grassland. The study area is generally considered to be overgrazed but has considerable variation in grass composition and basal cover. Sites included relatively intact natural grassland and sites where wattle infestations had been cleared and where mostly bare ground remained. I tested the hypotheses that overnight kraaling would result in (1) increased basal cover due to introduction of grass seed and stimulation of germination through hoof action, (2) increased infiltration due to hoof action, and (3) increased soil nutrients and organic matter due to dung and urine deposition. I also hypothesized that factors such as a site’s initial grass cover, its slope, the occurrence and amount of rainfall before and during kraaling, and the kraaling intensity (number of livestock and duration of the kraaling event) would influence the magnitude and direction of the kraaling effect. The effect of kraaling on vegetation was strongly dependent on initial condition. Kraaling increased basal cover of grasses when sites had low initial basal cover, but decreased basal cover if initial values were over 50%. Infiltration increased if kraaling took place during or after rain but decreased if kraaling took place when soils were dry. Kraaling increased soil P and K. In mesic grasslands, short-duration overnight kraaling is promising as a tool for rehabilitating degraded sites but should be avoided where the grass sward is relatively intact. I recommend that the suitability of kraaling be further evaluated per vegetation type and local context. , Thesis (MSc) -- Faculty of Science, Botany, 2023
- Full Text:
- Date Issued: 2023-10-13
The enigmatic subterranean amphipod genus (sternophysinx: sternophysingidae): conservation and systematics
- Authors: Mlungu, Zizile
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424424 , vital:72152
- Description: Sternophysingidae Holsinger, 1992 is a monotypic subterranean amphipod family endemic to Namibia and South Africa. It currently has eight described species under the genus Sternophysinx. The species in this family are considered narrow endemics, having only been identified using morphological characters. However, the apparent wide distribution of certain species, reported in several subterranean waters in different provinces, suggests that our current taxonomic understanding of these species needs to be completed, thus needing revision. Further, detailed knowledge of the distribution and phylogenetic relationship between species of this taxon still needs to be improved. This study aimed to use integrative taxonomy to analyse both morphology and molecular biology to study hidden diversity and to further our understanding of the distribution in this taxon. The available morphological keys were used to identify species, and 12S rRNA was also used to investigate the relationships of Sternophysinx species and delimit species boundaries. The study primarily aimed to use mitochondrial cytochrome c oxidase subunit I (COI). However, due to technical issues such as getting the correct primers, this study's aims were altered, and 12S was used instead of CO1. However, the process of getting CO1 sequences is still ongoing, and the results will be made available elsewhere. The current study aimed to visit all the type localities of the seven species of Sternophysinx reported in South Africa, including all known localities in which these amphipods have been recorded in the past. Nevertheless, this study did not get samples from Nelspruit, De Hoop Nature Reserve, and Nash Nature Reserve, which cover type localities of S. travaleensis, Matlapitse and Chaos cave that harbours S. calceola. Instead, the current study added five new localities Graaff-Reinet, Beaufort West, Hogsback, Makhambathi Nature Reserve and Ntsikeni Nature Reserve, for the distribution, including two new provinces (i.e., Eastern Cape and KwaZulu-Natal) that had records of this genus in the country. Three delimitation methods, Assemble Species by Automatic Partitioning (ASAP), Bayesian implementation of the Poison Tree Processor (bPTP) and Automatic Barcode Gap Discovery (ABGD), were used to identify cryptic species within Sternophysinx. Phylogenetic analyses and three delimitation methods (ASAP, bPTP and ABGD) revealed considerable hidden cryptic diversity within this Sternophysinx. Preliminary morphological examination revealed at least two species as undescribed from Bakwena and Sterkfontein caves, and several that were morphologically identified using the current taxonomic keys did not match with the molecular analysis. For example, S. basilobata from three different systems, Boesmansgat cave (Northern Cape), Beaufort West (Western Cape) and Potchefstroom (North West), was in genetically different putative species. The same is true for S. robertsi and S. alca in Ficus and Peppercorn cave in Limpopo. Overall, the results of the current study demonstrate the need for a comprehensive integrative taxonomic revision of Sternophysinx. These findings suggest that S. basilobata is a complex of distinct lineages and requires further study. On the other hand, species delimitation methods suggested that there are 12- 14 candidate species (Figure 2.5). Lastly, this study investigated the threat posed by the invasive koi fish introduced to an important cave, Boesmansgat cave in the Northern Cape, which is a type locality of two endemic species (S. megacheles and S. basilobata). Stable isotope (δ13C and δ15N) and gut content analyses of the fish were investigated to establish whether this invasive fish feed on these critical creatures, thus endangering their long-term survival. Stable isotope signatures and the stomach gut content proved to be helpful in determining the diet of the koi fish, revealing it to be an omnivorous feeder with high invertebrate preference, including amphipods. These results indicate that the introduction of koi fish in the Boesmansgat cave threatens the long-term sustainability of these amphipods and other invertebrate assemblages in this cave. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mlungu, Zizile
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424424 , vital:72152
- Description: Sternophysingidae Holsinger, 1992 is a monotypic subterranean amphipod family endemic to Namibia and South Africa. It currently has eight described species under the genus Sternophysinx. The species in this family are considered narrow endemics, having only been identified using morphological characters. However, the apparent wide distribution of certain species, reported in several subterranean waters in different provinces, suggests that our current taxonomic understanding of these species needs to be completed, thus needing revision. Further, detailed knowledge of the distribution and phylogenetic relationship between species of this taxon still needs to be improved. This study aimed to use integrative taxonomy to analyse both morphology and molecular biology to study hidden diversity and to further our understanding of the distribution in this taxon. The available morphological keys were used to identify species, and 12S rRNA was also used to investigate the relationships of Sternophysinx species and delimit species boundaries. The study primarily aimed to use mitochondrial cytochrome c oxidase subunit I (COI). However, due to technical issues such as getting the correct primers, this study's aims were altered, and 12S was used instead of CO1. However, the process of getting CO1 sequences is still ongoing, and the results will be made available elsewhere. The current study aimed to visit all the type localities of the seven species of Sternophysinx reported in South Africa, including all known localities in which these amphipods have been recorded in the past. Nevertheless, this study did not get samples from Nelspruit, De Hoop Nature Reserve, and Nash Nature Reserve, which cover type localities of S. travaleensis, Matlapitse and Chaos cave that harbours S. calceola. Instead, the current study added five new localities Graaff-Reinet, Beaufort West, Hogsback, Makhambathi Nature Reserve and Ntsikeni Nature Reserve, for the distribution, including two new provinces (i.e., Eastern Cape and KwaZulu-Natal) that had records of this genus in the country. Three delimitation methods, Assemble Species by Automatic Partitioning (ASAP), Bayesian implementation of the Poison Tree Processor (bPTP) and Automatic Barcode Gap Discovery (ABGD), were used to identify cryptic species within Sternophysinx. Phylogenetic analyses and three delimitation methods (ASAP, bPTP and ABGD) revealed considerable hidden cryptic diversity within this Sternophysinx. Preliminary morphological examination revealed at least two species as undescribed from Bakwena and Sterkfontein caves, and several that were morphologically identified using the current taxonomic keys did not match with the molecular analysis. For example, S. basilobata from three different systems, Boesmansgat cave (Northern Cape), Beaufort West (Western Cape) and Potchefstroom (North West), was in genetically different putative species. The same is true for S. robertsi and S. alca in Ficus and Peppercorn cave in Limpopo. Overall, the results of the current study demonstrate the need for a comprehensive integrative taxonomic revision of Sternophysinx. These findings suggest that S. basilobata is a complex of distinct lineages and requires further study. On the other hand, species delimitation methods suggested that there are 12- 14 candidate species (Figure 2.5). Lastly, this study investigated the threat posed by the invasive koi fish introduced to an important cave, Boesmansgat cave in the Northern Cape, which is a type locality of two endemic species (S. megacheles and S. basilobata). Stable isotope (δ13C and δ15N) and gut content analyses of the fish were investigated to establish whether this invasive fish feed on these critical creatures, thus endangering their long-term survival. Stable isotope signatures and the stomach gut content proved to be helpful in determining the diet of the koi fish, revealing it to be an omnivorous feeder with high invertebrate preference, including amphipods. These results indicate that the introduction of koi fish in the Boesmansgat cave threatens the long-term sustainability of these amphipods and other invertebrate assemblages in this cave. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
The ergonomic assessment of two different pipette models and their effects on biomechanical, performance, and subjective outcomes
- Authors: Masoka, Bonolo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424278 , vital:72139
- Description: While laboratory workers are essential in many industries, they are also at a high risk of developing musculoskeletal disorders (MSDs). Manual pipetting has been identified as the leading cause of MSDs in laboratories because it exposes operators to high forces, repetition, static muscular loading, and awkward wrist postures for prolonged periods. The main contributors to the high risk associated with manual pipetting are the design of manual pipettes and how they are used. A laboratory-based study was conducted to assess and compare the biomechanical, subjective, and performance responses of an ergonomically designed pipette model to those of a traditional model. In a simulated laboratory workstation, 20 individuals performed 40 repetitions of pipetting tasks using each pipette model. Dependent variables consisted of muscle activity (of the Abductor Pollicis Brevis, Opponens Pollicis, Extensor pollicis longus, Abductor pollicis longus, Extensor pollicis brevis, and brachioradialis muscles), wrist postures, time to task completion, perceived muscular exertion (measured using the Borg RPE scale), perceived comfort and usability, and user preference. The results revealed that an ergonomic modification to pipette design has some effect on biomechanical and subjective outcomes. The ergonomic model significantly reduced muscular loading of the Opponens pollicis, and Abductor pollicis brevis muscles. The ergonomic model also reduced radial deviation and was associated with better subjective responses, while the time to task completion did not change. This study found that an ergonomic modification to pipette design can improve user comfort and well-being without compromising performance. Overall, the findings emphasize the importance of addressing pipette design to reduce the MSD risk associated with manual pipetting, and the necessity for a user-centred approach to tool design. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Masoka, Bonolo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424278 , vital:72139
- Description: While laboratory workers are essential in many industries, they are also at a high risk of developing musculoskeletal disorders (MSDs). Manual pipetting has been identified as the leading cause of MSDs in laboratories because it exposes operators to high forces, repetition, static muscular loading, and awkward wrist postures for prolonged periods. The main contributors to the high risk associated with manual pipetting are the design of manual pipettes and how they are used. A laboratory-based study was conducted to assess and compare the biomechanical, subjective, and performance responses of an ergonomically designed pipette model to those of a traditional model. In a simulated laboratory workstation, 20 individuals performed 40 repetitions of pipetting tasks using each pipette model. Dependent variables consisted of muscle activity (of the Abductor Pollicis Brevis, Opponens Pollicis, Extensor pollicis longus, Abductor pollicis longus, Extensor pollicis brevis, and brachioradialis muscles), wrist postures, time to task completion, perceived muscular exertion (measured using the Borg RPE scale), perceived comfort and usability, and user preference. The results revealed that an ergonomic modification to pipette design has some effect on biomechanical and subjective outcomes. The ergonomic model significantly reduced muscular loading of the Opponens pollicis, and Abductor pollicis brevis muscles. The ergonomic model also reduced radial deviation and was associated with better subjective responses, while the time to task completion did not change. This study found that an ergonomic modification to pipette design can improve user comfort and well-being without compromising performance. Overall, the findings emphasize the importance of addressing pipette design to reduce the MSD risk associated with manual pipetting, and the necessity for a user-centred approach to tool design. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
- Full Text:
- Date Issued: 2023-10-13
The impacts of game farming on worker’s livelihoods: a case of a private game reserve in South Africa’s Eastern Cape Province
- Authors: Ngcoza, Nkosiphendule Mbasa
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425182 , vital:72217
- Description: Land use shifts from commercial agricultural farming to game farming through wildlife tourism, has been prevalent in post-apartheid South Africa. Scholars have argued that wildlife tourism to be more profitable than other forms of commercial farming. As such, game farming has been promoted as a community-engaged vehicle for rural development through the creation of better employment opportunities. This study explored the implications of these shifts on the livelihoods of farm workers living on the game farms. Unlike labour tenants, farm workers who live on the private game, earn a wage. However, farm workers’ accommodation on the game farm is dependent on them being employed on the farm. The aim of the study was to explore the impacts of game farms on the livelihoods of farm workers since the shift from commercial agricultural practices to tourism services in the form of private game reserves. A case study research design was used to gather in-depth information about the multiple realities of the participants, not only about their employment relationships, but also their experiences living on the property on which they are employed. I collected data mainly through semi-structured interviews. I also collected and analysed company documents. The study adopted a Sustainable Livelihoods Framework as a theoretical schema to analyse the livelihood assets readily available to the farm dwellers and the various livelihood strategies they used to make ends meet. The findings of the study revealed that residing on the game farm, although it provided access to free accommodation, tended to pose several challenges to the workers, including i) exploitative employment structures and ii) insecure land rights. The findings also revealed human capital development initiatives mandated for employees were not formally documented. As a result, employees were confined in their current employment. Consequently, the study revealed that participants who had been employed by the game farm for over a decade showed little evidence of enhanced livelihoods. The livelihood strategies of the participants were their basic employment wage, the use of money borrowing systems through stokvels, and informal trading such as selling sweets and chips in the game reserve villages where other workers live with their families. , Thesis (MA) -- Faculty of Humanities, Sociology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Ngcoza, Nkosiphendule Mbasa
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425182 , vital:72217
- Description: Land use shifts from commercial agricultural farming to game farming through wildlife tourism, has been prevalent in post-apartheid South Africa. Scholars have argued that wildlife tourism to be more profitable than other forms of commercial farming. As such, game farming has been promoted as a community-engaged vehicle for rural development through the creation of better employment opportunities. This study explored the implications of these shifts on the livelihoods of farm workers living on the game farms. Unlike labour tenants, farm workers who live on the private game, earn a wage. However, farm workers’ accommodation on the game farm is dependent on them being employed on the farm. The aim of the study was to explore the impacts of game farms on the livelihoods of farm workers since the shift from commercial agricultural practices to tourism services in the form of private game reserves. A case study research design was used to gather in-depth information about the multiple realities of the participants, not only about their employment relationships, but also their experiences living on the property on which they are employed. I collected data mainly through semi-structured interviews. I also collected and analysed company documents. The study adopted a Sustainable Livelihoods Framework as a theoretical schema to analyse the livelihood assets readily available to the farm dwellers and the various livelihood strategies they used to make ends meet. The findings of the study revealed that residing on the game farm, although it provided access to free accommodation, tended to pose several challenges to the workers, including i) exploitative employment structures and ii) insecure land rights. The findings also revealed human capital development initiatives mandated for employees were not formally documented. As a result, employees were confined in their current employment. Consequently, the study revealed that participants who had been employed by the game farm for over a decade showed little evidence of enhanced livelihoods. The livelihood strategies of the participants were their basic employment wage, the use of money borrowing systems through stokvels, and informal trading such as selling sweets and chips in the game reserve villages where other workers live with their families. , Thesis (MA) -- Faculty of Humanities, Sociology, 2023
- Full Text:
- Date Issued: 2023-10-13