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Babe uNkulunkulu

- Siswati church music workshop participants, Ngomozulu, Steven T., Dargie, Dave

Babe uNkulunkulu

- Siswati church music workshop participants, Composer not specfied, Dargie, Dave

Babe wethu

- Siswati church music workshop participants, Kabete, Themba T., Dargie, Dave

Bacteriophage growth on stationary phase achromabacter strains

- Robb, Susan Mary

  • Authors: Robb, Susan Mary
  • Date: 1980
  • Subjects: Bacteriophages , Strains and stresses
  • Language: English
  • Type: Thesis , Doctoral , PhD
  • Identifier: vital:4125 , http://hdl.handle.net/10962/d1014131
  • Description: Achromobacter w.t. and strain 14 both support phage α3a growth in stationary phase, but unlike the w.t. strain, exponential phase cultures of strain 14 block phage development. A standard method was developed for determining phage growth in stationary phase cultures. Lyophilised cells were used to eliminate variations due to the unstable phenotype of Achromobacter strain 14 cells. Phage α3a growth in stationary phase was characterized by a long and variable latent period of 6 to 9 h and an increased burst size of 709 p.f.u. per cell as compared with 153 p.f.u. per cell in exponential wild type cells. During the latent period the infected cells were very sensitive to changes in growth conditions and in particular, dilution. Pre-conditioning of the bacterial cells by allowing them to stand for 24 h after shaking for 3 days was an important aspect of the stationary phase phage growth system. Cells which had been allowed to stand retained the ability to be infected and to support phage growth for at least 16 days. Shaking cultures gradually lost the ability to support phage growth but the phage could persist in the host cell for 10 days until removal from shaking when the lytic cycle could proceed after allowing the cultures to stand. In comparison the latent period and burst size in Achromobacter w.t. stationary phase cells were reduced to less than 2 h and less than 200 respectively. Stationary phase cultures differed physiologically and morphologically depending on the aeration conditions. In comparison with non-aerated standing cultures, vigorously aerated cultures showed a decrease in viability, RNA synthesis, membrane transport, intracellular ATP levels, UV resistance and heat resistance but had markedly higher protein synthesis levels. Aerated cells were small non-motile rods which did not support phage growth. They developed into large motile rods under conditions of limited aeration and were able to propagate phage. It was proposed that changes in the host control mechanisms for macromolecular synthesis may be instrumental in either blocking or permitting phage development. A spontaneous mutant of Achromobacter strain 14 (14x) which liberated phage and was resistant to superinfection was isolated. The phage-host relationship was unstable and similar to the phage carrier state. The liberated phage were able to grow in exponential strain 14 cells. It was proposed that strain 14 was a defective lysogen and that an immunity phase shift model may account for the differential phage growth in exponential and stationary phase cells. Host transcriptional control appears to be implicated in control of phage development in exponential and stationary phase cells. Achromobacter Lp only supported phage in exponential phase but a rifampicin resistant mutant of this strain was able to propagate phage in stationary phase. In vitro RNA synthesis assays showed that the rifampicin resistance was caused by an alteration in the RNA polymerase. Preliminary experiments to determine intracellular phage macromolecular synthesis were carried out using exponential Achromobacter w.t. cells which had been irradiated with UV prior to infection. In irradiated cells, infection with phage resulted in stimulation of DNA synthesis but no stimulation of protein synthesis. Phage production was drastically reduced in cells which had been treated with very low UV doses. It was proposed that α3a development may rely heavily on host cell functions which are destroyed by UV. Achromobacter mutants with defective leucine transport systems were isolated. Mutants which lost the leucine uptake system completely were totally resistant to phage infection and were unable to adsorb phage α3a. This is the first report to implicate an amino-acid transport system in phage adsorption.
  • Full Text:
  • Date Issued: 1980

Bakriste

- Music workshop participants, Lucy, Sister, HCK, Mahlatsi, Monica, Mahlatsi, Emelia, Motwa, David, Letswalo, Anastacia, Mothwa, David, Letswalo, Chris, Tlhaka, William, Dargie, Dave

Baudelaire, nature and the artist in society

- Howell, Jane

  • Authors: Howell, Jane
  • Date: 1980
  • Subjects: Baudelaire, Charles, 1821-1867
  • Language: English
  • Type: Thesis , Masters , MFA
  • Identifier: vital:2466 , http://hdl.handle.net/10962/d1009432
  • Description: From Conclusion: The Artist can regard Baudelaire as a touchstone, as so many of his ideals and maxims are the ideals and maxims of the Artist himself. He teaches us many invaluable secrets of the universe and his lucid rendering of their explanations give us a clear insight into its mystery. He believed that Art was the ‘brainchild’ of Nature’s inspiration and that through its means and ways Natures mysteries will be revealed to us. He fought against all that the modern-day Artist is stiII fighting against. He rebelled against society1s false reasoning and its false morals. He became ‘self-exiled’ so that he could retain his individuality and reasoning. Like the Artist, his most valuable quality was his spontaneity and inspiration, given to him when his spirit moved him. His poems stand complete in themselves and yet all have a mysterious quality binding them. Likewise our paintings must also stand complete, they must be an end in themselves, each with its own singular message and yet a unity must prevaiI throughout. We must strive for that eternal quality that is so obvious in Baudelaire1s work. He can be read today at the distance of a century as if he had written for the present generation, with a knowledge of its problems and interests. His appeal is still vital because he was not fettered by the fashionable opinions and evanescent whims of his own age, and he made no concessions to the spirit of his own time in order to gain popularity.
  • Full Text:
  • Date Issued: 1980

Bawo wethu

- Lumko church choir for Silver Jubilee of father Oswald and father Fritz, Composer not specified, Dargie, Dave

Bawo wethu

- Lumko church choir for Silver Jubilee of father Oswald and father Fritz, Composer not specified, Dargie, Dave

Bawo wethu

- Lumko church choir for Silver Jubilee of father Oswald and father Fritz, Composer not specified, Dargie, Dave

Bawo Wethu

- Lumko Church Congregation, Composer not specified, Dargie, Dave

Bawo Wethu

- Lumko Church Congregation, Composer not specified, Dargie, Dave

Bawo Wethu

- Lumko Church Congregation, Composer not specified, Dargie, Dave

Bawo wethu

- Lumko church choir for Silver Jubilee of father Oswald and father Fritz, Composer not specified, Dargie, Dave

Bawo Wethu

- Siswati church music workshop participants, Mabuto, Nomahlozi R., Dargie, Dave

Bawo wethu = Our Father

- Manzini church congregation, Composer not specified, Dargie, Dave

Bawo wethu = Our Father

- Church congregation, Composer not specified, Dargie, Dave

Bayete

- Siswati church music workshop participants, Masango, M., Dargie, Dave

Big hill with houses in front a gravel road and fencing

Black urban employment and Coloured labour preference

- Bekker, S B, Coetzee, Johannes Hendrik

  • Authors: Bekker, S B , Coetzee, Johannes Hendrik
  • Date: 1980
  • Subjects: Black people -- Employment -- South Africa -- Cape Town Colored people (South Africa) -- Employment -- South Africa -- Cape Town Labor supply -- South Africa -- Cape Town Health services administration -- South Africa
  • Language: English
  • Type: Book , Text
  • Identifier: http://hdl.handle.net/10962/2216 , vital:20266 , ISBN 0868100420
  • Description: State control over the labour market usually results in the creation of different categories of labour, each having partial and unequal access to that market. In the Third World, typically, justification for control arises from an over-supply of unskilled rural labour and a small demand for such labour in the wage economy. To reduce massive urban unemployment, the state attempts to control the process of rural-urban migration by, inter alia, manipulating the labour market. ^ In South Africa, the civilised labour policy of the Pact government and the highly sophisticated system of black influx control introduced after the Second World War are two examples of such state control. In these cases, racial categories of labour, having differential access to the labour market, were created. The policy of Coloured Labour Preference is another South African example which is of particular interest since it is applied solely to one region of the Republic. Since 1962, when it was administratively coordinated for the first time, this policy has been applied in the Western Cape, a region comprising the 68 magisterial districts situated south-west of Port Elizabeth, Kimberley and the Orange River. In this region, black work-seekers' access to the labour market is severely curtailed. A series of regulations, particular to the Western Cape, are applied with the object of restricting the number of blacks resident in the region; denying blacks permanent rights of sojourn in the region; restricting the scope of employment for blacks in the region; and favouring coloured above black work-seekers throughout the region. In short, the policy aims to replace black by coloured labour and thereby aims to reduce to a minimum the number of blacks in the region. , Digitised by Rhodes University Library on behalf of the Institute of Social and Economic Research (ISER)
  • Full Text:
  • Date Issued: 1980

Butterworth, 1980

- Ingle, Pauline Cornwell, 1915-1999

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