Foam drug delivery in dermatology: beyond the scalp
- Purdon, Carryn H, Haigh, John M, Surber, Christian, Smith, Eric W
- Authors: Purdon, Carryn H , Haigh, John M , Surber, Christian , Smith, Eric W
- Date: 2003
- Subjects: Adis International Limited , Drug delivery systems , Skin disorders
- Language: English
- Type: text , Article
- Identifier: vital:6418 , http://hdl.handle.net/10962/d1006541
- Description: Consumers of topical formulations apply a wide spectrum of preparations, both cosmetic and dermatological, to their healthy or diseased skin. These formulations range in physicochemical nature from solid through semisolid to liquid. Pharmaceutical foams are pressurized dosage forms containing one or more active ingredients that, upon valve actuation, emit a fine dispersion of liquid and/or solid materials in a gaseous medium. Foam formulations are generally easier to apply, are less dense, and spread more easily than other topical dosage forms. Foams may be formulated in various ways to provide emollient or drying functions to the skin, depending on the formulation constituents. Therefore, this delivery technology should be a useful addition to the spectrum of formulations available for topical use; however, as yet, only a few are commercially available. Probably the most convincing argument for the use of foams is ease of use by the patient, and consumer acceptance. Most foam dosage forms used in dermatology to date have incorporated corticosteroids, although some products have also been used to deliver antiseptics, antifungal agents, anti-inflammatory agents, local anesthetic agents, skin emollients, and protectants. Although there is no clinical evidence that foam formulations are currently superior to other conventional delivery vehicles, these formulations have a clear application advantage and with continued developments in the science of supersaturation technology, it seems certain that foam delivery systems will retain their place in the dermatological and cosmetic armamentarium.
- Full Text:
- Date Issued: 2003
- Authors: Purdon, Carryn H , Haigh, John M , Surber, Christian , Smith, Eric W
- Date: 2003
- Subjects: Adis International Limited , Drug delivery systems , Skin disorders
- Language: English
- Type: text , Article
- Identifier: vital:6418 , http://hdl.handle.net/10962/d1006541
- Description: Consumers of topical formulations apply a wide spectrum of preparations, both cosmetic and dermatological, to their healthy or diseased skin. These formulations range in physicochemical nature from solid through semisolid to liquid. Pharmaceutical foams are pressurized dosage forms containing one or more active ingredients that, upon valve actuation, emit a fine dispersion of liquid and/or solid materials in a gaseous medium. Foam formulations are generally easier to apply, are less dense, and spread more easily than other topical dosage forms. Foams may be formulated in various ways to provide emollient or drying functions to the skin, depending on the formulation constituents. Therefore, this delivery technology should be a useful addition to the spectrum of formulations available for topical use; however, as yet, only a few are commercially available. Probably the most convincing argument for the use of foams is ease of use by the patient, and consumer acceptance. Most foam dosage forms used in dermatology to date have incorporated corticosteroids, although some products have also been used to deliver antiseptics, antifungal agents, anti-inflammatory agents, local anesthetic agents, skin emollients, and protectants. Although there is no clinical evidence that foam formulations are currently superior to other conventional delivery vehicles, these formulations have a clear application advantage and with continued developments in the science of supersaturation technology, it seems certain that foam delivery systems will retain their place in the dermatological and cosmetic armamentarium.
- Full Text:
- Date Issued: 2003
Paedophagia among cichlid fishes of Lake Victoria and Lake Malawi/Nyasa
- Ribbink, Aanthony J, Ribbink, A C
- Authors: Ribbink, Aanthony J , Ribbink, A C
- Date: 1997
- Language: English
- Type: text , Article
- Identifier: vital:7134 , http://hdl.handle.net/10962/d1011777
- Description: Paedophages, as defined here, are cichlids which exploit the protective tactics of other cichlids to steal the offspring from them. The different tactics are reviewed here and a new form of paedophagy is described, in which retrieval of free-swimming young is stimulated by the paedophage so that the offspring gather at the parent's mouth, where they are readily caught by the paedophage.
- Full Text: false
- Date Issued: 1997
- Authors: Ribbink, Aanthony J , Ribbink, A C
- Date: 1997
- Language: English
- Type: text , Article
- Identifier: vital:7134 , http://hdl.handle.net/10962/d1011777
- Description: Paedophages, as defined here, are cichlids which exploit the protective tactics of other cichlids to steal the offspring from them. The different tactics are reviewed here and a new form of paedophagy is described, in which retrieval of free-swimming young is stimulated by the paedophage so that the offspring gather at the parent's mouth, where they are readily caught by the paedophage.
- Full Text: false
- Date Issued: 1997
African Coelacanth Ecosystem Programme: An overview of the conference contributions
- Ribbink, Anthony J, Roberts, M
- Authors: Ribbink, Anthony J , Roberts, M
- Date: 2006
- Language: English
- Type: text , Article
- Identifier: vital:7127 , http://hdl.handle.net/10962/d1010637
- Description: Latimeria chalumnae is the icon for the multidisciplinary, multinational African Coelacanth Ecosystem Programme (ACEP) dedicated to improving the understanding of biological and other processes that support marine life. This article provides an overview of contributions made at a conference hosted by ACEP at the end of 2003. It also reviews significant developments regarding coelacanth conservation which have taken place since the conference. Delegates at the meeting concluded that the integrated regional,ecosystem approach that had been adopted by ACEP should continue. Underwater observation and exploration, however, should be supplemented by more experimental and technical analyses in order to answer longstanding questions related to coelacanths and other organisms.
- Full Text:
- Date Issued: 2006
- Authors: Ribbink, Anthony J , Roberts, M
- Date: 2006
- Language: English
- Type: text , Article
- Identifier: vital:7127 , http://hdl.handle.net/10962/d1010637
- Description: Latimeria chalumnae is the icon for the multidisciplinary, multinational African Coelacanth Ecosystem Programme (ACEP) dedicated to improving the understanding of biological and other processes that support marine life. This article provides an overview of contributions made at a conference hosted by ACEP at the end of 2003. It also reviews significant developments regarding coelacanth conservation which have taken place since the conference. Delegates at the meeting concluded that the integrated regional,ecosystem approach that had been adopted by ACEP should continue. Underwater observation and exploration, however, should be supplemented by more experimental and technical analyses in order to answer longstanding questions related to coelacanths and other organisms.
- Full Text:
- Date Issued: 2006
Predicting geographic distribution of seven blowfly species (Diptera: Calliphoridae) in South Africa
- Richards, C S, Williams, K A, Villet, Martin H
- Authors: Richards, C S , Williams, K A , Villet, Martin H
- Date: 2009
- Language: English
- Type: text , Article
- Identifier: vital:6854 , http://hdl.handle.net/10962/d1011135
- Description: The predicted geographic distributions of seven forensically important blowfly species are modelled using the computer program Maxent, based on selected climatic variables for South Africa, a country with large climatic and environmental gradients. It is shown that although temperature was hypothesized to most influence the distributions of these ectotherms, moisture, and particularly humidity, was in fact usually paramount. Chrysomya albiceps (Wiedemann) and C. marginalis (Robineau-Desvoidy) had the most widespread geographic and climatic distribution, while the forest-associated C. inclinata(Walker) was the least widespread. Chrysomya putoria (Wiedemann) and C. megacephala (Fabricius) had very similar predicted distributions that were restricted mainly to Limpopo, Mpumalanga, KwaZulu-Natal and the coast of the Eastern Cape. Chrysomya chloropyga (Wiedemann) and Calliphora croceipalpis (Jaennicke) were the only species predicted to occur at high altitudes. Blowfly distributions restricted to part of the map area were predicted better than those that were more widespread in the region, presumably because species with extremely widespread distributions in a study area occupy nearly the whole range of variation of most predictor variables, leaving little variation with which themaximumentropy modelling method can discriminate between presence and absence of the organism.
- Full Text:
- Date Issued: 2009
Predicting geographic distribution of seven blowfly species (Diptera: Calliphoridae) in South Africa
- Authors: Richards, C S , Williams, K A , Villet, Martin H
- Date: 2009
- Language: English
- Type: text , Article
- Identifier: vital:6854 , http://hdl.handle.net/10962/d1011135
- Description: The predicted geographic distributions of seven forensically important blowfly species are modelled using the computer program Maxent, based on selected climatic variables for South Africa, a country with large climatic and environmental gradients. It is shown that although temperature was hypothesized to most influence the distributions of these ectotherms, moisture, and particularly humidity, was in fact usually paramount. Chrysomya albiceps (Wiedemann) and C. marginalis (Robineau-Desvoidy) had the most widespread geographic and climatic distribution, while the forest-associated C. inclinata(Walker) was the least widespread. Chrysomya putoria (Wiedemann) and C. megacephala (Fabricius) had very similar predicted distributions that were restricted mainly to Limpopo, Mpumalanga, KwaZulu-Natal and the coast of the Eastern Cape. Chrysomya chloropyga (Wiedemann) and Calliphora croceipalpis (Jaennicke) were the only species predicted to occur at high altitudes. Blowfly distributions restricted to part of the map area were predicted better than those that were more widespread in the region, presumably because species with extremely widespread distributions in a study area occupy nearly the whole range of variation of most predictor variables, leaving little variation with which themaximumentropy modelling method can discriminate between presence and absence of the organism.
- Full Text:
- Date Issued: 2009
First steps in the development of a water temperature model framework for refining the ecological Reserve in South African rivers
- Rivers-Moore, N A, Hughes, Denis A, Mantel, Sukhmani K, Hill, Trevor R
- Authors: Rivers-Moore, N A , Hughes, Denis A , Mantel, Sukhmani K , Hill, Trevor R
- Date: 2008-10-05
- Language: English
- Type: text , Article
- Identifier: vital:7092 , http://hdl.handle.net/10962/d1012425
- Description: Ecological Reserve determination for rivers in South Africa presently does not include a water temperature component, in spite of its importance in determining species distribution patterns. To achieve this requires an understanding of how lotic thermographs from South African rivers differ from northern hemisphere rivers, to avoid mismanaging rivers based on incorrect regional assumptions. Hourly water temperatures from 20 sites in four river systems, representing a range of latitudes, altitudes and stream orders, were assessed using a range of metrics. These data were analysed using principal component analyses and multiple linear regressions to understand what variables a water temperature model for use in ecoregions within South Africa should include. While temperature data are generally lacking in low- and higher-order South African rivers, data suggest that South African rivers are warmer than northern hemisphere rivers. Water temperatures could be grouped into cool, warm and intermediate types. Based on temperature time series analyses, this paper argues that a suitable water-temperature model for use in ecological Reserve determinations should be dynamic, include flow and air temperature variables, and be adaptive through a heat exchange coefficient term. The inclusion of water temperature in the determination and management of river ecological Reserves would allow for more holistic application of the National Water Act's ecological management provisions. Water temperature guidelines added to the ecological Reserve could be integrated into heuristic aquatic monitoring programmes within priority areas identified in regional conservation plans.
- Full Text:
- Date Issued: 2008-10-05
- Authors: Rivers-Moore, N A , Hughes, Denis A , Mantel, Sukhmani K , Hill, Trevor R
- Date: 2008-10-05
- Language: English
- Type: text , Article
- Identifier: vital:7092 , http://hdl.handle.net/10962/d1012425
- Description: Ecological Reserve determination for rivers in South Africa presently does not include a water temperature component, in spite of its importance in determining species distribution patterns. To achieve this requires an understanding of how lotic thermographs from South African rivers differ from northern hemisphere rivers, to avoid mismanaging rivers based on incorrect regional assumptions. Hourly water temperatures from 20 sites in four river systems, representing a range of latitudes, altitudes and stream orders, were assessed using a range of metrics. These data were analysed using principal component analyses and multiple linear regressions to understand what variables a water temperature model for use in ecoregions within South Africa should include. While temperature data are generally lacking in low- and higher-order South African rivers, data suggest that South African rivers are warmer than northern hemisphere rivers. Water temperatures could be grouped into cool, warm and intermediate types. Based on temperature time series analyses, this paper argues that a suitable water-temperature model for use in ecological Reserve determinations should be dynamic, include flow and air temperature variables, and be adaptive through a heat exchange coefficient term. The inclusion of water temperature in the determination and management of river ecological Reserves would allow for more holistic application of the National Water Act's ecological management provisions. Water temperature guidelines added to the ecological Reserve could be integrated into heuristic aquatic monitoring programmes within priority areas identified in regional conservation plans.
- Full Text:
- Date Issued: 2008-10-05
Derivation of quantitative management objectives for annual instream water temperatures in the Sabie River using a biological index
- Rivers-Moore, N A, Jewitt, G P W, Weeks, D C
- Authors: Rivers-Moore, N A , Jewitt, G P W , Weeks, D C
- Date: 2005
- Language: English
- Type: text , Article
- Identifier: vital:7093 , http://hdl.handle.net/10962/d1012426
- Description: Adaptive management of river systems assumes uncertainty and makes provision for system variability. Inherent within this management approach is that perceived limits of 'acceptable' system variability are regarded not only as testable hypotheses, but also as playing a central role in maintaining biodiversity. While the Kruger National Park currently functions as a flagship conservation area in South Africa, projected increases in air temperatures as a consequence of global climate change present challenges in conserving this biodiversity inside the established land boundaries. Within the rivers of the Kruger National Park, a management goal of maintaining biodiversity requires a clearer understanding of system variability. One component of this is water temperature, an important water quality parameter defining the distribution patterns of aquatic organisms. In this study, Chiloglanis anoterus Crass (1960) (Pisces: Mochokidae) was selected as a biological indicator of changes in annual water temperatures within the Sabie River in the southern Kruger National Park. Relative abundances of C. anoterus were determined using standard electro-fishing surveys. The presence or absence of C. anoterus was linked to cumulative annual heat units using a logistic regression model, and a critical annual cumulative water temperature threshold estimated. A correlative relationship between this temperature threshold and a biological index using a C. anoterus condition factor provides river ecologists with a tool to assess ecologically significant warming trends in Sabie River water temperatures. A similar approach could be applied with relative ease to other Southern African river systems. Further testing of this hypothesis is suggested, as part of the adaptive management cycle.
- Full Text:
- Date Issued: 2005
- Authors: Rivers-Moore, N A , Jewitt, G P W , Weeks, D C
- Date: 2005
- Language: English
- Type: text , Article
- Identifier: vital:7093 , http://hdl.handle.net/10962/d1012426
- Description: Adaptive management of river systems assumes uncertainty and makes provision for system variability. Inherent within this management approach is that perceived limits of 'acceptable' system variability are regarded not only as testable hypotheses, but also as playing a central role in maintaining biodiversity. While the Kruger National Park currently functions as a flagship conservation area in South Africa, projected increases in air temperatures as a consequence of global climate change present challenges in conserving this biodiversity inside the established land boundaries. Within the rivers of the Kruger National Park, a management goal of maintaining biodiversity requires a clearer understanding of system variability. One component of this is water temperature, an important water quality parameter defining the distribution patterns of aquatic organisms. In this study, Chiloglanis anoterus Crass (1960) (Pisces: Mochokidae) was selected as a biological indicator of changes in annual water temperatures within the Sabie River in the southern Kruger National Park. Relative abundances of C. anoterus were determined using standard electro-fishing surveys. The presence or absence of C. anoterus was linked to cumulative annual heat units using a logistic regression model, and a critical annual cumulative water temperature threshold estimated. A correlative relationship between this temperature threshold and a biological index using a C. anoterus condition factor provides river ecologists with a tool to assess ecologically significant warming trends in Sabie River water temperatures. A similar approach could be applied with relative ease to other Southern African river systems. Further testing of this hypothesis is suggested, as part of the adaptive management cycle.
- Full Text:
- Date Issued: 2005
Prediction of water temperature metrics using spatial modelling in the Eastern and Western Cape, South Africa
- Rivers-Moore, N A, Mantel, S, Dallas, H F
- Authors: Rivers-Moore, N A , Mantel, S , Dallas, H F
- Date: 2012
- Language: English
- Type: text , Article
- Identifier: vital:7071 , http://hdl.handle.net/10962/d1003947 , http://www.scielo.org.za/scielo.php?pid=S1816-79502012000200002&script=sci_arttext
- Description: Key aspects of a river's temperature regime are described by magnitudes, timing and durations of thermal events, and frequencies of extreme exceedance events. To understand alterations to thermal regimes, it is necessary to describe thermal time series based on these statistics. Classification of sites based on their thermal metrics, and understanding of spatial patterns of these thermal statistics, provides a powerful approach for comparing study sites against reference sites. Water temperature regime dynamics should be viewed regionally, where regional divisions have an inherent underpinning by an understanding of natural thermal variability. The aim of this research was to link key water temperature metrics to readily-mapped environmental surrogates, and to produce spatial images of temperature metrics: 37 temperature metrics were derived for 12 months of sub-daily water temperatures at 90 sites in the Eastern Cape and Western Cape provinces, South Africa. These metrics were correlated with 16 environmental variables. Correlations enabled development of multiple regression models which facilitated mapping of temperature metrics over the study area. This approach has the potential to be applied at a national scale as more thermal time series are collected nationally. It is argued that the appropriateness of management decisions in rivers can be improved by including guidelines for thermal metrics at a regional scale. Such maps could facilitate incorporation of a temperature component into management guidelines for water resources.
- Full Text:
- Date Issued: 2012
- Authors: Rivers-Moore, N A , Mantel, S , Dallas, H F
- Date: 2012
- Language: English
- Type: text , Article
- Identifier: vital:7071 , http://hdl.handle.net/10962/d1003947 , http://www.scielo.org.za/scielo.php?pid=S1816-79502012000200002&script=sci_arttext
- Description: Key aspects of a river's temperature regime are described by magnitudes, timing and durations of thermal events, and frequencies of extreme exceedance events. To understand alterations to thermal regimes, it is necessary to describe thermal time series based on these statistics. Classification of sites based on their thermal metrics, and understanding of spatial patterns of these thermal statistics, provides a powerful approach for comparing study sites against reference sites. Water temperature regime dynamics should be viewed regionally, where regional divisions have an inherent underpinning by an understanding of natural thermal variability. The aim of this research was to link key water temperature metrics to readily-mapped environmental surrogates, and to produce spatial images of temperature metrics: 37 temperature metrics were derived for 12 months of sub-daily water temperatures at 90 sites in the Eastern Cape and Western Cape provinces, South Africa. These metrics were correlated with 16 environmental variables. Correlations enabled development of multiple regression models which facilitated mapping of temperature metrics over the study area. This approach has the potential to be applied at a national scale as more thermal time series are collected nationally. It is argued that the appropriateness of management decisions in rivers can be improved by including guidelines for thermal metrics at a regional scale. Such maps could facilitate incorporation of a temperature component into management guidelines for water resources.
- Full Text:
- Date Issued: 2012
Oceanographic environment of the Sodwana Bay coelacanths (Latimeria chalumnae), South Africa
- Roberts, M J, Ribbink, Anthony J, Morris, T
- Authors: Roberts, M J , Ribbink, Anthony J , Morris, T
- Date: 2006
- Language: English
- Type: text , Article
- Identifier: vital:7156 , http://hdl.handle.net/10962/d1011883
- Description: Trimix scuba divers discovered coelacanths in Jesser Canyon at a depth of 104 m on the northern KwaZulu-Natal (KZN) coast (Sodwana Bay) in October 2000. The existence of these animals at such a shallow depth and in the swift and powerful Agulhas Current led to a suggestion that this might be an isolated group swept well away from the main population in the Comoros, where they live at depths of 200–350 m with little current. Subsequent observations from three manned submersible surveys and one remotely operated vehicle expedition together with recreational diver observations indicate that the South African population of coelacanths has at least 26 individuals, mostly occupying the depth range of 104–140 m in canyons. Seventeen CTD sections collected during four cruises in 2002 and 2003 indicate the temperature range in this habitat to be similar to that found in the Comoros Islands (that is, 15–22°C cf. 15–19°C in the Comoros). However, a 2.5-month-long time series of hourly data collected by a thermistor array deployed near a known coelacanth cave in Wright Canyon indicated greater variation than anticipated, with temperature changes between 16°C and 24°C occurring in a day. Dissolved oxygen levels in this depth zone were found to range between 3.0 ml l[superscript (–1)] and 4.8 ml l[superscript (–1)] compared to 3.5 ml l[superscript (–1)] in the Comoros. The low oxygen values along this coast are a result of the shallow oxygen minimum, which becomes shallower in the southwest Indian Ocean, particularly in the Agulhas Current, than in tropical latitudes. Current velocities measured using a ship-borne ADCP in the depth range 100–140 m at Sodwana were considerably higher than those measured in the Comoros habitat (20–60 cm s[superscript (–1)] cf. 3–4 cm s[superscript (–1)]) and may be an important factor explaining the coelacanths’ occupation of the canyons found along the northern KZN shelf-break.
- Full Text:
- Date Issued: 2006
- Authors: Roberts, M J , Ribbink, Anthony J , Morris, T
- Date: 2006
- Language: English
- Type: text , Article
- Identifier: vital:7156 , http://hdl.handle.net/10962/d1011883
- Description: Trimix scuba divers discovered coelacanths in Jesser Canyon at a depth of 104 m on the northern KwaZulu-Natal (KZN) coast (Sodwana Bay) in October 2000. The existence of these animals at such a shallow depth and in the swift and powerful Agulhas Current led to a suggestion that this might be an isolated group swept well away from the main population in the Comoros, where they live at depths of 200–350 m with little current. Subsequent observations from three manned submersible surveys and one remotely operated vehicle expedition together with recreational diver observations indicate that the South African population of coelacanths has at least 26 individuals, mostly occupying the depth range of 104–140 m in canyons. Seventeen CTD sections collected during four cruises in 2002 and 2003 indicate the temperature range in this habitat to be similar to that found in the Comoros Islands (that is, 15–22°C cf. 15–19°C in the Comoros). However, a 2.5-month-long time series of hourly data collected by a thermistor array deployed near a known coelacanth cave in Wright Canyon indicated greater variation than anticipated, with temperature changes between 16°C and 24°C occurring in a day. Dissolved oxygen levels in this depth zone were found to range between 3.0 ml l[superscript (–1)] and 4.8 ml l[superscript (–1)] compared to 3.5 ml l[superscript (–1)] in the Comoros. The low oxygen values along this coast are a result of the shallow oxygen minimum, which becomes shallower in the southwest Indian Ocean, particularly in the Agulhas Current, than in tropical latitudes. Current velocities measured using a ship-borne ADCP in the depth range 100–140 m at Sodwana were considerably higher than those measured in the Comoros habitat (20–60 cm s[superscript (–1)] cf. 3–4 cm s[superscript (–1)]) and may be an important factor explaining the coelacanths’ occupation of the canyons found along the northern KZN shelf-break.
- Full Text:
- Date Issued: 2006
A fuzzy classification technique for predicting species' distributions: applications using invasive alien plants and indigenous insects
- Robertson, Mark P, Villet, Martin H, Palmer, Anthony R
- Authors: Robertson, Mark P , Villet, Martin H , Palmer, Anthony R
- Date: 2004
- Language: English
- Type: text , Article
- Identifier: vital:6897 , http://hdl.handle.net/10962/d1011659 , http://dx.doi.org/10.1111/j.1366-9516.2004.00108.x
- Description: A new predictive modelling technique called the fuzzy envelope model (FEM) is introduced. The technique can be used to predict potential distributions of organisms using presence-only locality records and a set of environmental predictor variables. FEM uses fuzzy logic to classify a set of predictor variable maps based on the values associated with presence records and combines the results to produce a potential distribution map for a target species. This technique represents several refinements of the envelope approach used in the BIOCLIM modelling package. These refinements are related to the way in which FEMs deal with uncertainty, the way in which this uncertainty is represented in the resultant potential distribution maps, and the way that these maps can be interpreted and applied. To illustrate its potential use in biogeographical studies, FEM was applied to predicting the potential distribution of three invasive alien plant species (Lantana camara L., Ricinus communis L. and Solanum mauritianum Scop.), and three native cicada species (Capicada decora Germar, Platypleura deusta Thun. and P. capensis L.) in South Africa, Lesotho and Swaziland. These models were quantitatively compared with models produced by means of the algorithm used in the BIOCLIM modelling package, which is referred to as a crisp envelope model (the CEM design). The average performance of models of the FEM design was consistently higher than those of the CEM design. There were significant differences in model performance among species but there was no significant interaction between model design and species. The average maximum kappa value ranged from 0.70 to 0.90 for FEM design and from 0.57 to 0.89 for the CEM design, which can be described as 'good' to 'excellent' using published ranges of agreement for the kappa statistic. This technique can be used to predict species' potential distributions that could be used for identifying regions at risk from invasion by alien species. These predictions could also be used in conservation planning in the case of native species.
- Full Text:
- Date Issued: 2004
- Authors: Robertson, Mark P , Villet, Martin H , Palmer, Anthony R
- Date: 2004
- Language: English
- Type: text , Article
- Identifier: vital:6897 , http://hdl.handle.net/10962/d1011659 , http://dx.doi.org/10.1111/j.1366-9516.2004.00108.x
- Description: A new predictive modelling technique called the fuzzy envelope model (FEM) is introduced. The technique can be used to predict potential distributions of organisms using presence-only locality records and a set of environmental predictor variables. FEM uses fuzzy logic to classify a set of predictor variable maps based on the values associated with presence records and combines the results to produce a potential distribution map for a target species. This technique represents several refinements of the envelope approach used in the BIOCLIM modelling package. These refinements are related to the way in which FEMs deal with uncertainty, the way in which this uncertainty is represented in the resultant potential distribution maps, and the way that these maps can be interpreted and applied. To illustrate its potential use in biogeographical studies, FEM was applied to predicting the potential distribution of three invasive alien plant species (Lantana camara L., Ricinus communis L. and Solanum mauritianum Scop.), and three native cicada species (Capicada decora Germar, Platypleura deusta Thun. and P. capensis L.) in South Africa, Lesotho and Swaziland. These models were quantitatively compared with models produced by means of the algorithm used in the BIOCLIM modelling package, which is referred to as a crisp envelope model (the CEM design). The average performance of models of the FEM design was consistently higher than those of the CEM design. There were significant differences in model performance among species but there was no significant interaction between model design and species. The average maximum kappa value ranged from 0.70 to 0.90 for FEM design and from 0.57 to 0.89 for the CEM design, which can be described as 'good' to 'excellent' using published ranges of agreement for the kappa statistic. This technique can be used to predict species' potential distributions that could be used for identifying regions at risk from invasion by alien species. These predictions could also be used in conservation planning in the case of native species.
- Full Text:
- Date Issued: 2004
The structure of aliphatic amine adducts of uranyl acetylacetonate. III. Dioxobis(2,4-pentanedionato)mono (2-N-isopropylaminopentan-4-one)uranium(VI)
- Rodgers, A L, Nassimbeni, L R, Haigh, John M
- Authors: Rodgers, A L , Nassimbeni, L R , Haigh, John M
- Date: 1977
- Language: English
- Type: text , Article
- Identifier: vital:6419 , http://hdl.handle.net/10962/d1006552
- Description: Introduction: In two earlier structural determinations of compounds of this type we have shown that the conformation of the adduct moiety is dependent on the formation of intramolecular N-H...0 hydrogen bonds (part I: Haigh, Nassimbeni, Pauptit, Rodgers & Sheldrick, 1976; part II: Nassimbeni, Orpen, Pauptit, Rodgers & Haigh, 1977). We have carried out the present analysis to study the conformational effects on the ligand brought about by the steric influence of an isopropyl substituent at N.
- Full Text:
- Date Issued: 1977
- Authors: Rodgers, A L , Nassimbeni, L R , Haigh, John M
- Date: 1977
- Language: English
- Type: text , Article
- Identifier: vital:6419 , http://hdl.handle.net/10962/d1006552
- Description: Introduction: In two earlier structural determinations of compounds of this type we have shown that the conformation of the adduct moiety is dependent on the formation of intramolecular N-H...0 hydrogen bonds (part I: Haigh, Nassimbeni, Pauptit, Rodgers & Sheldrick, 1976; part II: Nassimbeni, Orpen, Pauptit, Rodgers & Haigh, 1977). We have carried out the present analysis to study the conformational effects on the ligand brought about by the steric influence of an isopropyl substituent at N.
- Full Text:
- Date Issued: 1977
The structure of aliphatic amine adducts of uranyl acetylacetonate. IV. Dioxobis(2,4-pentanedionato) mono(2-aminopentan-4-one)uranium(VI)
- Rodgers, A L, Nassimbeni, L R, Pauptit, R A, Orpen, G, Haigh, John M
- Authors: Rodgers, A L , Nassimbeni, L R , Pauptit, R A , Orpen, G , Haigh, John M
- Date: 1977
- Language: English
- Type: text , Article
- Identifier: vital:6420 , http://hdl.handle.net/10962/d1006555
- Description: Introduction: We have shown in three earlier determinations of aliphatic amine adducts of U02(AA)2 (part I: Haigh, Nassimbeni, Pauptit, Rodgers & Sheldrick, 1976; part II: Nassimbeni, Orpen, Pauptit, Rodgers & Haigh, 1977; part III: Rodgers, Nassimbeni & Haigh, 1977) that the conformation of the adduct is dependent on its ability to form hydrogen bonds. The present compound has two H atoms available for hydrogen bonding and may be regarded as the parent of the series.
- Full Text:
- Date Issued: 1977
- Authors: Rodgers, A L , Nassimbeni, L R , Pauptit, R A , Orpen, G , Haigh, John M
- Date: 1977
- Language: English
- Type: text , Article
- Identifier: vital:6420 , http://hdl.handle.net/10962/d1006555
- Description: Introduction: We have shown in three earlier determinations of aliphatic amine adducts of U02(AA)2 (part I: Haigh, Nassimbeni, Pauptit, Rodgers & Sheldrick, 1976; part II: Nassimbeni, Orpen, Pauptit, Rodgers & Haigh, 1977; part III: Rodgers, Nassimbeni & Haigh, 1977) that the conformation of the adduct is dependent on its ability to form hydrogen bonds. The present compound has two H atoms available for hydrogen bonding and may be regarded as the parent of the series.
- Full Text:
- Date Issued: 1977
The development of a geomorphological classification system for the longitudinal zonation of South African rivers
- Rowntree, Kate, Wadeson, Roy A, O'Keeffe, Jay
- Authors: Rowntree, Kate , Wadeson, Roy A , O'Keeffe, Jay
- Date: 2000
- Language: English
- Type: text , Article
- Identifier: vital:7082 , http://hdl.handle.net/10962/d1012338
- Description: The recognition of the Reserve by the new South African Water Law poses new challenges for river scientists. The ecological water requirement or environmental flow is recognised by that part of the Reserve known as the ecological Reserve. If the ecological Reserve is to be implemented, it must first be defined and quantified for all river systems subject to water related developments. Standard procedures are being developed through the Resource Directed Measures (RDM) of the Department of Water Affairs and Forestry while monitoring the health of South Africa's rivers is taking place through the National River Health Programme (NRHP). Cost effective methods of developing spatial frameworks for both the RDM and NRHP are required. Concepts of longitudinal river zonation were developed by river ecologists in the 1960s and 1970s to assist in the classification of ecologically uniform stretches of rivers. This paper reviews the different zonation concepts and presents a geomorphological classification of South African river zones that is being applied to the RDM and NRHP. The classification is tested on three rivers, the Sabie, Buffalo and Olifants.
- Full Text: false
- Date Issued: 2000
- Authors: Rowntree, Kate , Wadeson, Roy A , O'Keeffe, Jay
- Date: 2000
- Language: English
- Type: text , Article
- Identifier: vital:7082 , http://hdl.handle.net/10962/d1012338
- Description: The recognition of the Reserve by the new South African Water Law poses new challenges for river scientists. The ecological water requirement or environmental flow is recognised by that part of the Reserve known as the ecological Reserve. If the ecological Reserve is to be implemented, it must first be defined and quantified for all river systems subject to water related developments. Standard procedures are being developed through the Resource Directed Measures (RDM) of the Department of Water Affairs and Forestry while monitoring the health of South Africa's rivers is taking place through the National River Health Programme (NRHP). Cost effective methods of developing spatial frameworks for both the RDM and NRHP are required. Concepts of longitudinal river zonation were developed by river ecologists in the 1960s and 1970s to assist in the classification of ecologically uniform stretches of rivers. This paper reviews the different zonation concepts and presents a geomorphological classification of South African river zones that is being applied to the RDM and NRHP. The classification is tested on three rivers, the Sabie, Buffalo and Olifants.
- Full Text: false
- Date Issued: 2000
Antiretroviral therapy in a South African public health care setting – facilitating and constraining factors
- Ruud, K W, Srinivas, Sunitha C, Toverud, E-L
- Authors: Ruud, K W , Srinivas, Sunitha C , Toverud, E-L
- Date: 2009
- Language: English
- Type: text , Article
- Identifier: vital:6453 , http://hdl.handle.net/10962/d1006831
- Description: The objective of the study was to identify and document facilitating and constraining factors in the antiretroviral therapy (ART) programme in a public health care setting in the Eastern Cape, South Africa. Observations for the study were carried out in a district hospital and two down-referral clinics in Makana Local Services Area in the Eastern Cape Province. Two discussion groups with key stakeholders were conducted to gather information about opinions and experiences among the health care providers (HCPs). It was found that the operating ART programme in this setting has been integrated in the existing down-referral health care system, based on follow-up in primary health care (PHC) clinics. Treatment is provided free of charge. The treatment programme provides the patients with access to counselling, nutritional assistance, psychosocial support and social welfare evaluation. However, increasing patient numbers and lack of human resources leads to a heavy workload for the HCPs involved with the ART programme. The need for additional, educated health workers is a major constraint for progress in provision of health care to patients who have accepted their HIV status, and are enrolled, or waiting to be enrolled, on the ART. However, delegation of work tasks among available HCPs and good communication between HCPs in the different clinics is a facilitating factor that ensures efficient use of the human resources available. Conclusion: Taking into account the challenges in a resource-constrained setting, this programme shows potential for functioning well as a provider of ART for those who are able and willing to access it. Considering an already heavy workload for HCPs, limitations and challenges still exist in reaching out with adequate treatment to a greater number of people who need ART.
- Full Text:
- Date Issued: 2009
- Authors: Ruud, K W , Srinivas, Sunitha C , Toverud, E-L
- Date: 2009
- Language: English
- Type: text , Article
- Identifier: vital:6453 , http://hdl.handle.net/10962/d1006831
- Description: The objective of the study was to identify and document facilitating and constraining factors in the antiretroviral therapy (ART) programme in a public health care setting in the Eastern Cape, South Africa. Observations for the study were carried out in a district hospital and two down-referral clinics in Makana Local Services Area in the Eastern Cape Province. Two discussion groups with key stakeholders were conducted to gather information about opinions and experiences among the health care providers (HCPs). It was found that the operating ART programme in this setting has been integrated in the existing down-referral health care system, based on follow-up in primary health care (PHC) clinics. Treatment is provided free of charge. The treatment programme provides the patients with access to counselling, nutritional assistance, psychosocial support and social welfare evaluation. However, increasing patient numbers and lack of human resources leads to a heavy workload for the HCPs involved with the ART programme. The need for additional, educated health workers is a major constraint for progress in provision of health care to patients who have accepted their HIV status, and are enrolled, or waiting to be enrolled, on the ART. However, delegation of work tasks among available HCPs and good communication between HCPs in the different clinics is a facilitating factor that ensures efficient use of the human resources available. Conclusion: Taking into account the challenges in a resource-constrained setting, this programme shows potential for functioning well as a provider of ART for those who are able and willing to access it. Considering an already heavy workload for HCPs, limitations and challenges still exist in reaching out with adequate treatment to a greater number of people who need ART.
- Full Text:
- Date Issued: 2009
Analysis of the calling songs of Platypleura hirtipennis (Germar, 1834) and P. plumosa (Germar, 1834) (Hemiptera: Cicadidae)
- Sanborn, Allen F, Phillips, P K, Villet, Martin H
- Authors: Sanborn, Allen F , Phillips, P K , Villet, Martin H
- Date: 2003
- Language: English
- Type: text , Article
- Identifier: vital:6896 , http://hdl.handle.net/10962/d1011656
- Description: [From the introduction]: Most male cicadas produce a calling song in order to attract conspecific females. These songs have been shown to differ in closely related species (e.g. Alexander & Moore 1962; Villet 1988, 1989; Boulard 1995; Marshall & Cooley 2000; Sanborn & Phillips 2001) and in species which share habitats (e.g. Sueur 2002). The former is an inevitable part of the divergence of recognition signals that characterizes the speciation process in animals using acoustic signals (Villet 1995), while the latter would be expected from a signal that acts as a reproductive isolating mechanism (Claridge 1985; Marshall & Cooley 2000). Calling songs are therefore of value in resolving taxonomic problems in the cicadas.
- Full Text:
- Date Issued: 2003
- Authors: Sanborn, Allen F , Phillips, P K , Villet, Martin H
- Date: 2003
- Language: English
- Type: text , Article
- Identifier: vital:6896 , http://hdl.handle.net/10962/d1011656
- Description: [From the introduction]: Most male cicadas produce a calling song in order to attract conspecific females. These songs have been shown to differ in closely related species (e.g. Alexander & Moore 1962; Villet 1988, 1989; Boulard 1995; Marshall & Cooley 2000; Sanborn & Phillips 2001) and in species which share habitats (e.g. Sueur 2002). The former is an inevitable part of the divergence of recognition signals that characterizes the speciation process in animals using acoustic signals (Villet 1995), while the latter would be expected from a signal that acts as a reproductive isolating mechanism (Claridge 1985; Marshall & Cooley 2000). Calling songs are therefore of value in resolving taxonomic problems in the cicadas.
- Full Text:
- Date Issued: 2003
Endothermy in African Platypleurine Cicadas: the influence of body size and habitat (Hemiptera: Cicadidae)
- Sanborn, Allen F, Villet, Martin H, Phillips, P K
- Authors: Sanborn, Allen F , Villet, Martin H , Phillips, P K
- Date: 2004
- Language: English
- Type: text , Article
- Identifier: vital:6925 , http://hdl.handle.net/10962/d1011912
- Description: The platypleurine cicadas have a wide distribution across Africa and southern Asia. We investigate endothermy as a thermoregulatory strategy in 11 South African species from five genera, with comparisons to the lone ectothermic platypleurine we found, in an attempt to ascertain any influence that habitat and/or body size have on the expression of endothermy in the platypleurine cicadas. Field measurements of body temperature (Tb) show that these animals regulate Tb through endogenous heat production. Heat production in the laboratory elevated Tb to the same range as in animals active in the field. Maximum Tb measured during calling activity when there was no access to solar radiation ranged from 13.2° to 22.3°C above ambient temperature in the five species measured. The mean Tb during activity without access to solar radiation did not differ from the mean Tb during diurnal activity. All platypleurines exhibit a unique behavior for cicadas while warming endogenously, a temperature- dependent telescoping pulsation of the abdomen that probably functions in ventilation. Platypleurines generally call from trunks and branches within the canopy and appear to rely on endothermy even when the sun is available to elevate Tb, in contrast to the facultative endothermy exhibited by New World endothermic species. The two exceptions to this generalization we found within the platypleurines are Platypleura wahlbergi and Albanycada albigera, which were the smallest species studied. The small size of P. wahlbergi appears to have altered their thermoregulatory strategy to one of facultative endothermy, whereby they use the sun when it is available to facilitate increases in Tb. Albanycada albigera is the only ectothermic platypleurine we found. The habitat and host plant association of A. albigera appear to have influenced the choice of ectothermy as a thermoregulatory strategy, as the species possesses the metabolic machinery to elevate to the Tb range observed in the endothermic species. Therefore, size and habitat appear to influence the expression of thermoregulatory strategies in African platypleurine cicadas.
- Full Text:
- Date Issued: 2004
- Authors: Sanborn, Allen F , Villet, Martin H , Phillips, P K
- Date: 2004
- Language: English
- Type: text , Article
- Identifier: vital:6925 , http://hdl.handle.net/10962/d1011912
- Description: The platypleurine cicadas have a wide distribution across Africa and southern Asia. We investigate endothermy as a thermoregulatory strategy in 11 South African species from five genera, with comparisons to the lone ectothermic platypleurine we found, in an attempt to ascertain any influence that habitat and/or body size have on the expression of endothermy in the platypleurine cicadas. Field measurements of body temperature (Tb) show that these animals regulate Tb through endogenous heat production. Heat production in the laboratory elevated Tb to the same range as in animals active in the field. Maximum Tb measured during calling activity when there was no access to solar radiation ranged from 13.2° to 22.3°C above ambient temperature in the five species measured. The mean Tb during activity without access to solar radiation did not differ from the mean Tb during diurnal activity. All platypleurines exhibit a unique behavior for cicadas while warming endogenously, a temperature- dependent telescoping pulsation of the abdomen that probably functions in ventilation. Platypleurines generally call from trunks and branches within the canopy and appear to rely on endothermy even when the sun is available to elevate Tb, in contrast to the facultative endothermy exhibited by New World endothermic species. The two exceptions to this generalization we found within the platypleurines are Platypleura wahlbergi and Albanycada albigera, which were the smallest species studied. The small size of P. wahlbergi appears to have altered their thermoregulatory strategy to one of facultative endothermy, whereby they use the sun when it is available to facilitate increases in Tb. Albanycada albigera is the only ectothermic platypleurine we found. The habitat and host plant association of A. albigera appear to have influenced the choice of ectothermy as a thermoregulatory strategy, as the species possesses the metabolic machinery to elevate to the Tb range observed in the endothermic species. Therefore, size and habitat appear to influence the expression of thermoregulatory strategies in African platypleurine cicadas.
- Full Text:
- Date Issued: 2004
Thermal responses in some Eastern Cape African Cicadas (Hemiptera: Cicadidae)
- Sanborn, Allen F, Phillips, P K, Villet, Martin H
- Authors: Sanborn, Allen F , Phillips, P K , Villet, Martin H
- Date: 2003
- Language: English
- Type: text , Article
- Identifier: vital:6918 , http://hdl.handle.net/10962/d1011881
- Description: Thermal responses were measured in cicadas collected in the Eastern Cape Province of South Africa. The thermal responses of 22 species from 5 biomes were determined. Shade-seeking temperature was the most variable and related to the various biomes. Mean shade-seeking temperature was greatest for species inhabiting the thicket biome and lowest for species inhabiting the forest biome. The animals that live in the thicket biome may adapt to the greater thermal stress to take advantage of a habitat that permits lower predation pressure. There is a correlation between body size and shade-seeking temperatures with smaller species exhibiting lower thermal responses within a particular habitat. This may be related to the greater heat exchange in smaller species. Heat torpor temperatures did not differ between the various biomes.
- Full Text:
- Date Issued: 2003
- Authors: Sanborn, Allen F , Phillips, P K , Villet, Martin H
- Date: 2003
- Language: English
- Type: text , Article
- Identifier: vital:6918 , http://hdl.handle.net/10962/d1011881
- Description: Thermal responses were measured in cicadas collected in the Eastern Cape Province of South Africa. The thermal responses of 22 species from 5 biomes were determined. Shade-seeking temperature was the most variable and related to the various biomes. Mean shade-seeking temperature was greatest for species inhabiting the thicket biome and lowest for species inhabiting the forest biome. The animals that live in the thicket biome may adapt to the greater thermal stress to take advantage of a habitat that permits lower predation pressure. There is a correlation between body size and shade-seeking temperatures with smaller species exhibiting lower thermal responses within a particular habitat. This may be related to the greater heat exchange in smaller species. Heat torpor temperatures did not differ between the various biomes.
- Full Text:
- Date Issued: 2003
Hot-blooded singers: endothermy facilitates crepuscular signaling in African platypleurine cicadas (Hemiptera: Cicadidae: Platypleura spp.)
- Sanborn, Allen F, Villet, Martin H, Phillips, P K
- Authors: Sanborn, Allen F , Villet, Martin H , Phillips, P K
- Date: 2003
- Language: English
- Type: text , Article
- Identifier: vital:6919 , http://hdl.handle.net/10962/d1011882
- Description: The cicada genus Platypleura has a wide distribution across Africa and southern Asia. We describe endothermic thermoregulation in four South African species that show crepuscular signaling behavior. This is the first evidence of thermoregulation in platypleurine cicadas. Field measurements of body temperature (Tb) show that these animals regulate Tb through endogenous heat production. Maximum Tb measured was 22.1°C above ambient temperature during calling activity at dusk. The mean Tb during dusk activity did not differ from the mean Tb during diurnal activity. A unique behavior for cicadas, a temperature-dependent telescoping pulsation of the abdomen, was observed in the laboratory during endogenous warm-up. This behavior is part of a unique method of heat generation in endothermic cicadas. Males generally call from trunks and branches within the canopy and appear to use endothermy even when the sun is available to elevate Tb. Endothermy may provide the cicadas with the advantage of decreasing predation and acoustic competition by permitting calling from perches that most complement their cryptic coloration patterns and that ectotherms cannot use due to thermal constraints. In addition, endothermy may permit calling activity during crepuscular hours when atmospheric conditions are optimal for acoustic communication and predation risks are minimal.
- Full Text:
- Date Issued: 2003
- Authors: Sanborn, Allen F , Villet, Martin H , Phillips, P K
- Date: 2003
- Language: English
- Type: text , Article
- Identifier: vital:6919 , http://hdl.handle.net/10962/d1011882
- Description: The cicada genus Platypleura has a wide distribution across Africa and southern Asia. We describe endothermic thermoregulation in four South African species that show crepuscular signaling behavior. This is the first evidence of thermoregulation in platypleurine cicadas. Field measurements of body temperature (Tb) show that these animals regulate Tb through endogenous heat production. Maximum Tb measured was 22.1°C above ambient temperature during calling activity at dusk. The mean Tb during dusk activity did not differ from the mean Tb during diurnal activity. A unique behavior for cicadas, a temperature-dependent telescoping pulsation of the abdomen, was observed in the laboratory during endogenous warm-up. This behavior is part of a unique method of heat generation in endothermic cicadas. Males generally call from trunks and branches within the canopy and appear to use endothermy even when the sun is available to elevate Tb. Endothermy may provide the cicadas with the advantage of decreasing predation and acoustic competition by permitting calling from perches that most complement their cryptic coloration patterns and that ectotherms cannot use due to thermal constraints. In addition, endothermy may permit calling activity during crepuscular hours when atmospheric conditions are optimal for acoustic communication and predation risks are minimal.
- Full Text:
- Date Issued: 2003
Application of satellite-derived rainfall estimates to extend water resource simulation modelling in South Africa
- Sawunyama, Tendai, Hughes, Denis A
- Authors: Sawunyama, Tendai , Hughes, Denis A
- Date: 2008
- Language: English
- Type: text , Article
- Identifier: vital:7089 , http://hdl.handle.net/10962/d1012419
- Description: Spatially interpolated rainfall estimates from rain-gauges are widely used as input to hydrological models, but deriving accurate estimates at appropriate space and time scales remain a major problem. In South Africa there has been a gradual decrease in the number of active rain-gauges over time. Satellite-based estimates of spatial rainfall are becoming more readily available and offer a viable substitute. The paper presents the potential of using Climate Prediction Center African daily precipitation climatology (CPCAPC) satellite-based datasets (2001-2006) to drive a Pitman hydrological model which has been calibrated using gauge-based rainfall data (1920-1990). However, if two sources of rainfall data are to be used together, it is necessary to ensure that they are compatible in terms of their statistical properties. A non-linear frequency of exceedance transformation technique was used to correct the satellite data to be more consistent with historical spatial rainfall estimates. The technique generated simulation results for the 2001 to 2006 period that were greatly improved compared to the direct use of the untransformed satellite data. While there remain some further questions about the use of satellite-derived rainfall data in different parts of the country, they do seem to have the potential to contribute to extending water resource modelling into the future.
- Full Text:
- Date Issued: 2008
- Authors: Sawunyama, Tendai , Hughes, Denis A
- Date: 2008
- Language: English
- Type: text , Article
- Identifier: vital:7089 , http://hdl.handle.net/10962/d1012419
- Description: Spatially interpolated rainfall estimates from rain-gauges are widely used as input to hydrological models, but deriving accurate estimates at appropriate space and time scales remain a major problem. In South Africa there has been a gradual decrease in the number of active rain-gauges over time. Satellite-based estimates of spatial rainfall are becoming more readily available and offer a viable substitute. The paper presents the potential of using Climate Prediction Center African daily precipitation climatology (CPCAPC) satellite-based datasets (2001-2006) to drive a Pitman hydrological model which has been calibrated using gauge-based rainfall data (1920-1990). However, if two sources of rainfall data are to be used together, it is necessary to ensure that they are compatible in terms of their statistical properties. A non-linear frequency of exceedance transformation technique was used to correct the satellite data to be more consistent with historical spatial rainfall estimates. The technique generated simulation results for the 2001 to 2006 period that were greatly improved compared to the direct use of the untransformed satellite data. While there remain some further questions about the use of satellite-derived rainfall data in different parts of the country, they do seem to have the potential to contribute to extending water resource modelling into the future.
- Full Text:
- Date Issued: 2008
Analysis of chromameter results obtained from corticosteroid-induced skin blanching assay: comparison of visual and chromameter data
- Schwarb, Fabian P, Smith, Eric W, Haigh, John M, Surber, Christian
- Authors: Schwarb, Fabian P , Smith, Eric W , Haigh, John M , Surber, Christian
- Date: 1999
- Language: English
- Type: text , Article
- Identifier: vital:6426 , http://hdl.handle.net/10962/d1006562
- Description: In a Guidance document, the American FDA recommends the use of a Minolta chromameter rather than the human eye for the quantitative assessment of the pharmacodynamic blanching response produced by topical application of corticosteroids. The purpose of this study was to compare the appropriateness of the human eye and two models of chromameter for the estimation of skin blanching, in terms of the quality of the data generated by each method. The corticosteroid-induced skin blanching from four different betamethasone 17-valerate cream formulations was compared in a typical human skin blanching trial. The optimized assay methodology routinely practised in our laboratories was utilized. The blanching responses were assessed visually by three trained, independent observers and recorded by two chromameters (Minolta model CR-200 and model CR-300). The topical availability of the four creams was determined using visual scoring and chromameter measurements. All data were manipulated in such a manner as to produce a blanching response versus time profile from which AUBC analysis could be performed. Good correlation was observed between the visual assessments made by three independent observers. In contrast, moderate correlation was determined between visual, CR-200 and CR-300 measurements. Surprisingly, no direct linear relationship between the AUBCs produced by the two chromameters was observed indicating that the quality of the data obtained from the two instruments may not be equal. This investigation also indicated that the use of the chromameter is not completely objective. Visual scoring and chromameter measurement produce data sets that differ in quality. Each procedure needs to be validated and investigators have to be trained for both visual assessment and the operation of the chromameter, particularly with regard to the manipulation of the measuring head of the instrument.
- Full Text:
- Date Issued: 1999
- Authors: Schwarb, Fabian P , Smith, Eric W , Haigh, John M , Surber, Christian
- Date: 1999
- Language: English
- Type: text , Article
- Identifier: vital:6426 , http://hdl.handle.net/10962/d1006562
- Description: In a Guidance document, the American FDA recommends the use of a Minolta chromameter rather than the human eye for the quantitative assessment of the pharmacodynamic blanching response produced by topical application of corticosteroids. The purpose of this study was to compare the appropriateness of the human eye and two models of chromameter for the estimation of skin blanching, in terms of the quality of the data generated by each method. The corticosteroid-induced skin blanching from four different betamethasone 17-valerate cream formulations was compared in a typical human skin blanching trial. The optimized assay methodology routinely practised in our laboratories was utilized. The blanching responses were assessed visually by three trained, independent observers and recorded by two chromameters (Minolta model CR-200 and model CR-300). The topical availability of the four creams was determined using visual scoring and chromameter measurements. All data were manipulated in such a manner as to produce a blanching response versus time profile from which AUBC analysis could be performed. Good correlation was observed between the visual assessments made by three independent observers. In contrast, moderate correlation was determined between visual, CR-200 and CR-300 measurements. Surprisingly, no direct linear relationship between the AUBCs produced by the two chromameters was observed indicating that the quality of the data obtained from the two instruments may not be equal. This investigation also indicated that the use of the chromameter is not completely objective. Visual scoring and chromameter measurement produce data sets that differ in quality. Each procedure needs to be validated and investigators have to be trained for both visual assessment and the operation of the chromameter, particularly with regard to the manipulation of the measuring head of the instrument.
- Full Text:
- Date Issued: 1999
Effect of concentration and degree of saturation of topical fluocinonide formulations on in vitro membrane transport and in vivo availability on human skin
- Schwarb, Fabian P, Imanidis, Georgios, Smith, Eric W, Haigh, John M, Surber, Christian
- Authors: Schwarb, Fabian P , Imanidis, Georgios , Smith, Eric W , Haigh, John M , Surber, Christian
- Date: 1999
- Language: English
- Type: text , Article
- Identifier: vital:6425 , http://hdl.handle.net/10962/d1006560
- Description: Purpose. The thermodynamic acitvity of drugs in topical vehicles is considered to significantly influence topical delivery. In vitro diffusion across a synthetic membrane was shown to be correlated to the degree of saturation of the drug in the applied vehicle and therefore offers a potential for increased topical drug delivery. Fluocinonide a topical corticosteroid, was chosen as a model compound to investigate in vitro and in vivo availability from formulations with different degrees of saturation. Methods. Sub-, as well as, supersaturated drug solutions were prepared using PVP as an antinucleant agent. In vitro membrane diffusion experiments across silicone membrane and in vivo pharmacodynamic activity assessments, using the human skin blanching assay, were carried out. Results. Over the concentration range studied, the in vitro membrane transport of fluocinonide was proportional to the degree of saturation of the respective formulations. The in vivo pharmacodynamic response in the human skin blanching assay was related to the concentration of the drug in the vehicle irrespective of the degree of saturation. Conclusions. From the membrane permeation experiment it can be concluded, that the drug flux might be increased supra-proportionally with increasing donor concentration, drug (super-)saturation (proportional), beyond what would be anticipated based on ideal donor concentration and partition coefficient considerations only. These findings could not be confirmed in the in vivo investigation, probably due to additional vehicle effects (e.g., enhancement, irritation, drug binding) which have to be expected and could have altered the integrity of the stratum corneum and therewith topical bioavailability of the drug.
- Full Text: false
- Date Issued: 1999
- Authors: Schwarb, Fabian P , Imanidis, Georgios , Smith, Eric W , Haigh, John M , Surber, Christian
- Date: 1999
- Language: English
- Type: text , Article
- Identifier: vital:6425 , http://hdl.handle.net/10962/d1006560
- Description: Purpose. The thermodynamic acitvity of drugs in topical vehicles is considered to significantly influence topical delivery. In vitro diffusion across a synthetic membrane was shown to be correlated to the degree of saturation of the drug in the applied vehicle and therefore offers a potential for increased topical drug delivery. Fluocinonide a topical corticosteroid, was chosen as a model compound to investigate in vitro and in vivo availability from formulations with different degrees of saturation. Methods. Sub-, as well as, supersaturated drug solutions were prepared using PVP as an antinucleant agent. In vitro membrane diffusion experiments across silicone membrane and in vivo pharmacodynamic activity assessments, using the human skin blanching assay, were carried out. Results. Over the concentration range studied, the in vitro membrane transport of fluocinonide was proportional to the degree of saturation of the respective formulations. The in vivo pharmacodynamic response in the human skin blanching assay was related to the concentration of the drug in the vehicle irrespective of the degree of saturation. Conclusions. From the membrane permeation experiment it can be concluded, that the drug flux might be increased supra-proportionally with increasing donor concentration, drug (super-)saturation (proportional), beyond what would be anticipated based on ideal donor concentration and partition coefficient considerations only. These findings could not be confirmed in the in vivo investigation, probably due to additional vehicle effects (e.g., enhancement, irritation, drug binding) which have to be expected and could have altered the integrity of the stratum corneum and therewith topical bioavailability of the drug.
- Full Text: false
- Date Issued: 1999