Eco-ethology of shell-dwelling cichlids in Lake Tanganyika
- Authors: Bills, Ian Roger
- Date: 1997
- Subjects: Cichlids -- Africa, East , Cichlids -- Tanganyika, Lake , Fishes -- Behavior
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5265 , http://hdl.handle.net/10962/d1005109 , Cichlids -- Africa, East , Cichlids -- Tanganyika, Lake , Fishes -- Behavior
- Description: Observations of habitats are reported. A series of underwater experiments were conducted in natural habitats to answer questions concerning a) why Lamprologus ocellatus and Lamprologus ornatipinnis bury gastropod shells refuges into the substrate, and b) to examine interspecies differences in shell-using behaviours. Some behaviour patterns were analysed using phylogenetic methods. Lamprologus ocellatus and L. ornatipinnis responded to new shells in a variety of ways, shells were moved, buried (and used) or hidden (buried and not used). How shells are utilised seems to be dependant on a complex of factors such as the size and quality of new the shell and the number already in the territory. Shell use may also be affected by neighbour species, sex, size and predation levels. There are interspecific differences in the size of shells used and the methods of shell use. The latter results in species-characteristic shell orientations, vertical burial in L. ocellatus and horizontal burial in L. ornatipinnis. Shell orientation does affect other species/use of shells. Shell movement and vertical orientation appear to be apomorphic while shell hiding and burial are pleisiomorphic within the genus Lamprologus. Numerous cues are involved in stimulating shell burial. Most of these cues are actively sought by the fish by external and internal inspections. Shell burial therefore appears to be a method of reducing the information gathering ability of potential shell-dwelling competitors. Shell burial can therefore be regarded as an investment process which enhances the residents ability to defend its territory. Males can also control the distribution of open shells within teritories and thus control mate access to shells. This behaviour could be a significant factor in the evolution of marked sexual dichromatism exhibited within the genus.
- Full Text:
- Authors: Bills, Ian Roger
- Date: 1997
- Subjects: Cichlids -- Africa, East , Cichlids -- Tanganyika, Lake , Fishes -- Behavior
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5265 , http://hdl.handle.net/10962/d1005109 , Cichlids -- Africa, East , Cichlids -- Tanganyika, Lake , Fishes -- Behavior
- Description: Observations of habitats are reported. A series of underwater experiments were conducted in natural habitats to answer questions concerning a) why Lamprologus ocellatus and Lamprologus ornatipinnis bury gastropod shells refuges into the substrate, and b) to examine interspecies differences in shell-using behaviours. Some behaviour patterns were analysed using phylogenetic methods. Lamprologus ocellatus and L. ornatipinnis responded to new shells in a variety of ways, shells were moved, buried (and used) or hidden (buried and not used). How shells are utilised seems to be dependant on a complex of factors such as the size and quality of new the shell and the number already in the territory. Shell use may also be affected by neighbour species, sex, size and predation levels. There are interspecific differences in the size of shells used and the methods of shell use. The latter results in species-characteristic shell orientations, vertical burial in L. ocellatus and horizontal burial in L. ornatipinnis. Shell orientation does affect other species/use of shells. Shell movement and vertical orientation appear to be apomorphic while shell hiding and burial are pleisiomorphic within the genus Lamprologus. Numerous cues are involved in stimulating shell burial. Most of these cues are actively sought by the fish by external and internal inspections. Shell burial therefore appears to be a method of reducing the information gathering ability of potential shell-dwelling competitors. Shell burial can therefore be regarded as an investment process which enhances the residents ability to defend its territory. Males can also control the distribution of open shells within teritories and thus control mate access to shells. This behaviour could be a significant factor in the evolution of marked sexual dichromatism exhibited within the genus.
- Full Text:
An evaluation of recirculating artificial stream designs for acute toxicity testing using two South African Ephemeroptera species exposed to sodium sulphate
- Authors: Binder, Markus
- Date: 2000
- Subjects: Water -- Pollution -- Toxicology , Toxicology -- Research , Aquatic organisms -- Effect of water pollution on , Sodium sulfate , Mayflies
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5690 , http://hdl.handle.net/10962/d1005376 , Water -- Pollution -- Toxicology , Toxicology -- Research , Aquatic organisms -- Effect of water pollution on , Sodium sulfate , Mayflies
- Description: Three artificial stream designs, termed Large Artificial Stream Units (LASUs), Raceways, and Channels, at two major scales (1700 L, 12.5 L and 20 L recirculated volume) were developed at the Institute for Water Research, Rhodes University, Grahamstown, in order to explore the possibilities of using indigenous rheophilic macroinvertebrates in routine toxicity tests. This study compared these systems, using 96h-EC50 values from sodium sulphate toxicity tests as the experimental response. Two local Ephemeroptera (Leptophlebiidae: Adenophlebia auriculata Eaton, and Baetidae: Afroptilum sudafricanum Lestage) were evaluated for their suitability in routine toxicity tests; and the possible effects of elevated salinity levels in South African rivers on the test species were assessed. Two sets of experiments with each mayfly species were conducted, following an unreplicated regression design. Dechlorinated tap water was used as the water source. Experiments in the Channels were repeated to determine experimental variability. Results were compared statistically by testing for overlap of 95% confidence limits (95%Cls) of EC50 values. The differences between A. auriculata EC50 values in the different systems were statistically significant (no overlap of 95%CLs), but they were not more variable than has been considered normal for biological systems (Coefficient of variation 20.1 %; ratio of greatest EC50 / smallest EC50 1.63). The differences were not related to the scale or the average current velocity characteristic of each stream design (average current velocity LASUs - Raceways - Channels 0.090 - 0.083 - 0.038 m/s). The Channels proved to be most efficient with regard to practical performance as they are portable and easily transportable, user-friendly, reliable, splash-free, cost effective to construct, and can easily be adapted to specific requirements. These systems are therefore recommended for regular use. The suitability of the two mayfly species for routine toxicity testing was evaluated. A. auriculata EC50 values showed a significant negative correlation with the corresponding average body-size (range 1476 - 1610 μm, mean 1555 μm). The different average body-sizes probably reflected the abundance of a certain size range present in the Palmiet River at the time of collection. Both species reacted similarly to Na₂S0₄ (similar slopes of the toxicity curves), identifying this salt as a slow acting toxicant. A. sudafricanum populations were more sensitive to Na₂S0₄ (EC50 3.404 g/L) than A. auriculata (EC50 8.090 g/L), probably because of its smaller body-size (mean 709 μm) and a lack of extremely tolerant individuals. In comparison to other freshwater macro invertebrates, including the standard toxicity test organism Daphnia spp., both mayfly species seemed to be moderately tolerant of Na₂S0₄; therefore there was no particular advantage to using these indigenous taxa rather than Daphnia spp. An assessment of the effects of elevated salinity/TDS levels on the test taxa yielded preliminary insights. A NaCI-EC50 for A. sudafricanum could be extrapolated and suggested a higher sensitivity to Na₂S0₄ than to NaCl. When Na₂S0₄ EC50 values of both species were compared to selected TDS levels of South African rivers, 4. auriculata would mostly not be affected, but A. sudafricanum might occasionally suffer from sub-lethal effects, depending on the sulphate proportion of the TDS. The South African guideline for TDS seemed to protect both species sufficiently.
- Full Text:
- Authors: Binder, Markus
- Date: 2000
- Subjects: Water -- Pollution -- Toxicology , Toxicology -- Research , Aquatic organisms -- Effect of water pollution on , Sodium sulfate , Mayflies
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5690 , http://hdl.handle.net/10962/d1005376 , Water -- Pollution -- Toxicology , Toxicology -- Research , Aquatic organisms -- Effect of water pollution on , Sodium sulfate , Mayflies
- Description: Three artificial stream designs, termed Large Artificial Stream Units (LASUs), Raceways, and Channels, at two major scales (1700 L, 12.5 L and 20 L recirculated volume) were developed at the Institute for Water Research, Rhodes University, Grahamstown, in order to explore the possibilities of using indigenous rheophilic macroinvertebrates in routine toxicity tests. This study compared these systems, using 96h-EC50 values from sodium sulphate toxicity tests as the experimental response. Two local Ephemeroptera (Leptophlebiidae: Adenophlebia auriculata Eaton, and Baetidae: Afroptilum sudafricanum Lestage) were evaluated for their suitability in routine toxicity tests; and the possible effects of elevated salinity levels in South African rivers on the test species were assessed. Two sets of experiments with each mayfly species were conducted, following an unreplicated regression design. Dechlorinated tap water was used as the water source. Experiments in the Channels were repeated to determine experimental variability. Results were compared statistically by testing for overlap of 95% confidence limits (95%Cls) of EC50 values. The differences between A. auriculata EC50 values in the different systems were statistically significant (no overlap of 95%CLs), but they were not more variable than has been considered normal for biological systems (Coefficient of variation 20.1 %; ratio of greatest EC50 / smallest EC50 1.63). The differences were not related to the scale or the average current velocity characteristic of each stream design (average current velocity LASUs - Raceways - Channels 0.090 - 0.083 - 0.038 m/s). The Channels proved to be most efficient with regard to practical performance as they are portable and easily transportable, user-friendly, reliable, splash-free, cost effective to construct, and can easily be adapted to specific requirements. These systems are therefore recommended for regular use. The suitability of the two mayfly species for routine toxicity testing was evaluated. A. auriculata EC50 values showed a significant negative correlation with the corresponding average body-size (range 1476 - 1610 μm, mean 1555 μm). The different average body-sizes probably reflected the abundance of a certain size range present in the Palmiet River at the time of collection. Both species reacted similarly to Na₂S0₄ (similar slopes of the toxicity curves), identifying this salt as a slow acting toxicant. A. sudafricanum populations were more sensitive to Na₂S0₄ (EC50 3.404 g/L) than A. auriculata (EC50 8.090 g/L), probably because of its smaller body-size (mean 709 μm) and a lack of extremely tolerant individuals. In comparison to other freshwater macro invertebrates, including the standard toxicity test organism Daphnia spp., both mayfly species seemed to be moderately tolerant of Na₂S0₄; therefore there was no particular advantage to using these indigenous taxa rather than Daphnia spp. An assessment of the effects of elevated salinity/TDS levels on the test taxa yielded preliminary insights. A NaCI-EC50 for A. sudafricanum could be extrapolated and suggested a higher sensitivity to Na₂S0₄ than to NaCl. When Na₂S0₄ EC50 values of both species were compared to selected TDS levels of South African rivers, 4. auriculata would mostly not be affected, but A. sudafricanum might occasionally suffer from sub-lethal effects, depending on the sulphate proportion of the TDS. The South African guideline for TDS seemed to protect both species sufficiently.
- Full Text:
Human-river relationships in the Kat River catchment and the implications for integrated water resource management (IWRM) : an exploraratory study
- Authors: Birkholz, Sharon Alice
- Date: 2009
- Subjects: Aquatic ecology -- South Africa -- Kat River , Rivers -- South Africa -- Kat River -- Sociological aspects , Rivers -- South Africa -- Kat River , Water supply -- South Africa -- Kat River -- Management , Human ecology -- South Africa -- Kat River , Stream ecology -- South Africa -- Kat River
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: vital:4838 , http://hdl.handle.net/10962/d1005514
- Description: Through out this study ‘relational scenarios’ were seen as the possible outcomes of the expression of human-river relationships in a catchment. Working within Inglis’s (2008, pg. 10) comment that ‘the Human World Relationship is expressed through a person’s views and behaviour towards the natural world, which can be either constructive or destructive’, two relational scenarios were selected to represent these two predicted outcomes of human-environment (or in the case of this thesis human-river) relationships: IWRM, seen as constructive and mutually beneficial to both the social and ecological system, and the Tragedy of the Commons, seen as destructive and parasitic in nature. In respect to this assertion, a conceptual framework or model was developed and used to guide the inductive process of this research. Through a social survey (administered via semi-structured interviews) of stakeholders (water-users) in the Kat River Catchment, Eastern Cape, South Africa, social attitudes towards the Kat River were explored. These attitudes were considered as variables that relate to the expression of ‘Human World Relationships’ namely ‘values and behaviour’ (Inglis, 2008, pg. 10). The findings of this thesis confirm that attitudes are influential components of human-river relationships in the Kat River Catchment. It was observed from the findings that the attitudes individuals have towards the Kat River have the potential to influence the ways people interact with the River and its associated resources, and are in turn influenced by the condition of the River and connections people have or make with the River within their daily lives. Such relationships in turn affect the management of the River and its water resources and influence how individuals approach water-related issues and undertake the associated activities. Given this understanding it was then proposed that attitudes could provide information that enables the prediction of likely ‘relational scenarios’ (IWRM or the Tragedy of the Commons) in a catchment. The findings appear to support this proposal, and three main attitude groups (utilitarian, associative and dissociative) were identified and used to further the discussion into how this knowledge could be used in predicting possible relational scenarios based on human-river relationships. Further exploration of the identified attitude groups suggested that these variables are significantly influenced by environmental ethics and place attachment (present in the community) and a series of guiding frameworks were developed to facilitate the exploration of the attitude groups relative to these concepts. The frameworks were generated from the hypothesis that knowledge of attitude groups in a catchment, in connection with the relative degrees to which moral values and worldviews are expressed, could provide insight into the readiness of a catchment system for the implementation of IWRM and/or the facilitative steps needed to shift the influence of unfavourable attitude groups (i.e. dissociative attitudes). Such steps would most likely involve extensive educational, awareness and capacity building programs. Finally, given the above theoretical frameworks, inductively developed from the findings of the social survey and related literature, the conceptual model was reevaluated and extended to include the thesis findings and hypotheses. It is suggested that in a catchment where there is a balance between the expression of utilitarian and associative attitude groups, then there is a high probability of finding present evidence of mutually beneficial human-river relationships already in play, as well as ‘fertile soil’ for the promotion of IWRM and philosophies and skills that generate such relationships. Alternatively in an area with a strong expression of dissociative attitudes, it is more likely to find evidence of destructive, more parasitic-like relationships being expressed, and a lack of commitment and interest in being involved in changing the status quo. It is likely that where both associative and dissociative attitudes are present there will be an overlap of elements of both scenarios – a situation that is probable in most catchments – depending on the strength of expression of either attitude group the balance will shift (be shifting) between the two relational scenarios.
- Full Text:
- Authors: Birkholz, Sharon Alice
- Date: 2009
- Subjects: Aquatic ecology -- South Africa -- Kat River , Rivers -- South Africa -- Kat River -- Sociological aspects , Rivers -- South Africa -- Kat River , Water supply -- South Africa -- Kat River -- Management , Human ecology -- South Africa -- Kat River , Stream ecology -- South Africa -- Kat River
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: vital:4838 , http://hdl.handle.net/10962/d1005514
- Description: Through out this study ‘relational scenarios’ were seen as the possible outcomes of the expression of human-river relationships in a catchment. Working within Inglis’s (2008, pg. 10) comment that ‘the Human World Relationship is expressed through a person’s views and behaviour towards the natural world, which can be either constructive or destructive’, two relational scenarios were selected to represent these two predicted outcomes of human-environment (or in the case of this thesis human-river) relationships: IWRM, seen as constructive and mutually beneficial to both the social and ecological system, and the Tragedy of the Commons, seen as destructive and parasitic in nature. In respect to this assertion, a conceptual framework or model was developed and used to guide the inductive process of this research. Through a social survey (administered via semi-structured interviews) of stakeholders (water-users) in the Kat River Catchment, Eastern Cape, South Africa, social attitudes towards the Kat River were explored. These attitudes were considered as variables that relate to the expression of ‘Human World Relationships’ namely ‘values and behaviour’ (Inglis, 2008, pg. 10). The findings of this thesis confirm that attitudes are influential components of human-river relationships in the Kat River Catchment. It was observed from the findings that the attitudes individuals have towards the Kat River have the potential to influence the ways people interact with the River and its associated resources, and are in turn influenced by the condition of the River and connections people have or make with the River within their daily lives. Such relationships in turn affect the management of the River and its water resources and influence how individuals approach water-related issues and undertake the associated activities. Given this understanding it was then proposed that attitudes could provide information that enables the prediction of likely ‘relational scenarios’ (IWRM or the Tragedy of the Commons) in a catchment. The findings appear to support this proposal, and three main attitude groups (utilitarian, associative and dissociative) were identified and used to further the discussion into how this knowledge could be used in predicting possible relational scenarios based on human-river relationships. Further exploration of the identified attitude groups suggested that these variables are significantly influenced by environmental ethics and place attachment (present in the community) and a series of guiding frameworks were developed to facilitate the exploration of the attitude groups relative to these concepts. The frameworks were generated from the hypothesis that knowledge of attitude groups in a catchment, in connection with the relative degrees to which moral values and worldviews are expressed, could provide insight into the readiness of a catchment system for the implementation of IWRM and/or the facilitative steps needed to shift the influence of unfavourable attitude groups (i.e. dissociative attitudes). Such steps would most likely involve extensive educational, awareness and capacity building programs. Finally, given the above theoretical frameworks, inductively developed from the findings of the social survey and related literature, the conceptual model was reevaluated and extended to include the thesis findings and hypotheses. It is suggested that in a catchment where there is a balance between the expression of utilitarian and associative attitude groups, then there is a high probability of finding present evidence of mutually beneficial human-river relationships already in play, as well as ‘fertile soil’ for the promotion of IWRM and philosophies and skills that generate such relationships. Alternatively in an area with a strong expression of dissociative attitudes, it is more likely to find evidence of destructive, more parasitic-like relationships being expressed, and a lack of commitment and interest in being involved in changing the status quo. It is likely that where both associative and dissociative attitudes are present there will be an overlap of elements of both scenarios – a situation that is probable in most catchments – depending on the strength of expression of either attitude group the balance will shift (be shifting) between the two relational scenarios.
- Full Text:
Age, growth, reproduction and sexual dimorphism of the striped dolphin, Stenella coeruleoalba, off the south-east coast of southern Africa
- Authors: Bishop, Amy Ruth
- Date: 2014
- Subjects: Striped dolphin -- Conservation -- Africa, Southern , Striped dolphin -- Development -- Africa, Southern , Striped dolphin -- Africa, Southern -- Reproduction , Striped dolphin -- Age determination -- Africa, Southern , Dolphins -- Conservation -- Africa, Southern , Dolphins -- Development -- Africa, Southern , Dolphins -- Africa, Southern -- Reproduction , Dolphins -- Age determination -- Africa, Southern , Dimorphism (Animals)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5848 , http://hdl.handle.net/10962/d1011600 , Striped dolphin -- Conservation -- Africa, Southern , Striped dolphin -- Development -- Africa, Southern , Striped dolphin -- Africa, Southern -- Reproduction , Striped dolphin -- Age determination -- Africa, Southern , Dolphins -- Conservation -- Africa, Southern , Dolphins -- Development -- Africa, Southern , Dolphins -- Africa, Southern -- Reproduction , Dolphins -- Age determination -- Africa, Southern , Dimorphism (Animals)
- Description: The striped dolphin, Stenella coeruleoalba, is an oceanic dolphin found worldwide in tropical to warm temperate waters. Globally populations are threatened through direct fishing, bycatch and pollution. Little is known about the life history of this species in South African waters and it is currently listed as ‘Data Deficient’ by the International Union for Conservation of Nature (IUCN). Critical to the conservation and management of this species is an understanding of basic life history parameters. For the present study, metadata and tissues from 101 stranded S. coeruleoalba along the south-east coast of southern Africa between 1969 and 2012 were analyzed to examine age and growth, male and female reproduction and sexual dimorphism. Age and growth were determined by counting the number of growth layer groups (GLG’s) present in their teeth. A von Bertalanffy growth curve gave the best fit to the data and indicated that physical maturity was reached around 21 years in males and 18 years in females. Additionally, asymptotic lengths were reached at 231cm and 224cm in males and females, respectively. The maximum ages obtained were 21 years for males and 20 years for females. Length at birth was estimated to be between 116cm and 119cm for males and between 112cm and 114cm for females. The reproductive status of the adult dolphins was assessed by histological and macroscopic analyses of gonadal tissue. Sexual maturity was attained before physical maturity in both sexes. The males reached sexual maturity between 8 and 12 years of age and between 209 and 224cm in total body length. The maximum combined testis weight comprised about 0.24% of the total body weight and sexual maturity was reached between a combined testis weight of 16 and 41g, a mean testis length of 8 and 10cm, and a mean seminiferous tubule diameter of 44 and 70μm. Females attained sexual maturity between 7 and 8 years of age and between 213 and 216 cm in total body length. The ovulation rate was 0.32 per year and the majority of ovulations occurred in the left ovary, with ovulations occurring in the right ovary only in individuals older than 10 years of age. The annual pregnancy rate was calculated to be 26%. Reproductive seasonality could not be established due to the small sample size and the absence of samples throughout the year. However, both conceptions and births appear to occur in both August and December/January. Additionally, testis weight and seminiferous tubule diameters appear to fluctuate throughout the year, which could suggest reproductive seasonality in this species. Sexual size dimorphism in mature adults was evident for a number of external measurements, however, sexual shape dimorphism was not present in this species. Mature males had significantly longer and wider pectoral fins, dorsal fins and flukes than females (p<0.05 in all cases). It was suggested that the larger appendages are required for male on male combat during intraspecific competition for females. Additionally, larger appendages aid in thermoregulation, propulsion and maneuverability. Females had a significantly greater distance between the tip upper jaw and the genital aperture and anus, which was expected due to differences in anatomy between male and female genital openings. Overall, S. coeruleoalba did not exhibit distinct sexual dimorphism and together with the relatively small testes in relation to the total body weight (0.24%) it was proposed that this species demonstrates a less extreme form of polygyny. Additionaly, the results of this study are important to the understanding of the basic biology of the species.
- Full Text:
- Authors: Bishop, Amy Ruth
- Date: 2014
- Subjects: Striped dolphin -- Conservation -- Africa, Southern , Striped dolphin -- Development -- Africa, Southern , Striped dolphin -- Africa, Southern -- Reproduction , Striped dolphin -- Age determination -- Africa, Southern , Dolphins -- Conservation -- Africa, Southern , Dolphins -- Development -- Africa, Southern , Dolphins -- Africa, Southern -- Reproduction , Dolphins -- Age determination -- Africa, Southern , Dimorphism (Animals)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5848 , http://hdl.handle.net/10962/d1011600 , Striped dolphin -- Conservation -- Africa, Southern , Striped dolphin -- Development -- Africa, Southern , Striped dolphin -- Africa, Southern -- Reproduction , Striped dolphin -- Age determination -- Africa, Southern , Dolphins -- Conservation -- Africa, Southern , Dolphins -- Development -- Africa, Southern , Dolphins -- Africa, Southern -- Reproduction , Dolphins -- Age determination -- Africa, Southern , Dimorphism (Animals)
- Description: The striped dolphin, Stenella coeruleoalba, is an oceanic dolphin found worldwide in tropical to warm temperate waters. Globally populations are threatened through direct fishing, bycatch and pollution. Little is known about the life history of this species in South African waters and it is currently listed as ‘Data Deficient’ by the International Union for Conservation of Nature (IUCN). Critical to the conservation and management of this species is an understanding of basic life history parameters. For the present study, metadata and tissues from 101 stranded S. coeruleoalba along the south-east coast of southern Africa between 1969 and 2012 were analyzed to examine age and growth, male and female reproduction and sexual dimorphism. Age and growth were determined by counting the number of growth layer groups (GLG’s) present in their teeth. A von Bertalanffy growth curve gave the best fit to the data and indicated that physical maturity was reached around 21 years in males and 18 years in females. Additionally, asymptotic lengths were reached at 231cm and 224cm in males and females, respectively. The maximum ages obtained were 21 years for males and 20 years for females. Length at birth was estimated to be between 116cm and 119cm for males and between 112cm and 114cm for females. The reproductive status of the adult dolphins was assessed by histological and macroscopic analyses of gonadal tissue. Sexual maturity was attained before physical maturity in both sexes. The males reached sexual maturity between 8 and 12 years of age and between 209 and 224cm in total body length. The maximum combined testis weight comprised about 0.24% of the total body weight and sexual maturity was reached between a combined testis weight of 16 and 41g, a mean testis length of 8 and 10cm, and a mean seminiferous tubule diameter of 44 and 70μm. Females attained sexual maturity between 7 and 8 years of age and between 213 and 216 cm in total body length. The ovulation rate was 0.32 per year and the majority of ovulations occurred in the left ovary, with ovulations occurring in the right ovary only in individuals older than 10 years of age. The annual pregnancy rate was calculated to be 26%. Reproductive seasonality could not be established due to the small sample size and the absence of samples throughout the year. However, both conceptions and births appear to occur in both August and December/January. Additionally, testis weight and seminiferous tubule diameters appear to fluctuate throughout the year, which could suggest reproductive seasonality in this species. Sexual size dimorphism in mature adults was evident for a number of external measurements, however, sexual shape dimorphism was not present in this species. Mature males had significantly longer and wider pectoral fins, dorsal fins and flukes than females (p<0.05 in all cases). It was suggested that the larger appendages are required for male on male combat during intraspecific competition for females. Additionally, larger appendages aid in thermoregulation, propulsion and maneuverability. Females had a significantly greater distance between the tip upper jaw and the genital aperture and anus, which was expected due to differences in anatomy between male and female genital openings. Overall, S. coeruleoalba did not exhibit distinct sexual dimorphism and together with the relatively small testes in relation to the total body weight (0.24%) it was proposed that this species demonstrates a less extreme form of polygyny. Additionaly, the results of this study are important to the understanding of the basic biology of the species.
- Full Text:
The feeding ecology, habitat selection and hunting behaviour of re-introduced cheetah on Kwandwe Private Game Reserve, Eastern Cape Province
- Authors: Bissett, Charlene
- Date: 2005
- Subjects: Kwandwe Game Reserve (South Africa) , Cheetah -- Behavior -- South Africa -- Eastern Cape , Cheetah -- Nutrition -- South Africa -- Eastern Cape , Cheetah -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5698 , http://hdl.handle.net/10962/d1005384 , Kwandwe Game Reserve (South Africa) , Cheetah -- Behavior -- South Africa -- Eastern Cape , Cheetah -- Nutrition -- South Africa -- Eastern Cape , Cheetah -- South Africa -- Eastern Cape
- Description: The re-introduction of cheetah onto small reserves in the Eastern Cape Province has created the opportunity to study this species in an environment (Valley Bushveld) in which it has not previously been studied and which is quite different from the less wooded habitats in which previous studies have occurred. Thus the aim of this study is to add to the growing number of studies of cheetah in more wooded habitats and to contribute a better understanding of the level of specialization or adaptability in space use, habitat selection, diet and hunting behaviour that the cheetah possesses. The research was conducted on Kwandwe Private Game Reserve from February 2003 to August 2004. Direct observations, scat analysis and continuous observations were used to avoid bias towards large sized prey found when only using direct observations to study diet. The cheetah killed 15 species and four of the five most important prey species (kudu, springbok, grey duiker and bushbuck) were hunted according to their abundance on the reserve, while impala, the fifth most important prey species, was avoided (i.e. preference index less than one) by the cheetah. The cheetah’s main prey was medium sized ungulates, although the three male coalition killed larger prey than females and females with cubs. The daily food intake per cheetah per day was calculated to range from 5.3kg/cheetah/day for the coalition to 8kg/cheetah/day for a solitary female. Home range areas ranged from 11.1 km² for female cheetah with cubs in a den to 65.6 km² for single female cheetah and core areas ranged from 6 km² for the three male coalition to 26.5 km² for independent cubs. Habitat selection by cheetah on Kwandwe varied between the social groups depending on their susceptibility to predation by lions, their need for cover and need for water, and was similar to what has been previously reported. The home range of the coalition incorporated the most open vegetation type (karroid shrubland) with surrounding denser vegetation, while females occupied areas of denser vegetation. Activity patterns and hunting behaviour varied between different cheetah social groups with female cheetah being more active during day light hours compared to males which made 38% of their kills after dark. The average chase distances for the various cheetah groups varied considerably, and the chase distance for successful hunts was longer than for unsuccessful for all groups except single female cheetah. The percentage of kills’ kleptoparasitised on Kwandwe was very low compared to other studies possibly due to the low density of direct competitors, which in turn lead to longer mean kill retention times. These results suggest that cheetah are more adaptable than previously thought and this adaptability may have important implications for their conservation.
- Full Text:
- Authors: Bissett, Charlene
- Date: 2005
- Subjects: Kwandwe Game Reserve (South Africa) , Cheetah -- Behavior -- South Africa -- Eastern Cape , Cheetah -- Nutrition -- South Africa -- Eastern Cape , Cheetah -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5698 , http://hdl.handle.net/10962/d1005384 , Kwandwe Game Reserve (South Africa) , Cheetah -- Behavior -- South Africa -- Eastern Cape , Cheetah -- Nutrition -- South Africa -- Eastern Cape , Cheetah -- South Africa -- Eastern Cape
- Description: The re-introduction of cheetah onto small reserves in the Eastern Cape Province has created the opportunity to study this species in an environment (Valley Bushveld) in which it has not previously been studied and which is quite different from the less wooded habitats in which previous studies have occurred. Thus the aim of this study is to add to the growing number of studies of cheetah in more wooded habitats and to contribute a better understanding of the level of specialization or adaptability in space use, habitat selection, diet and hunting behaviour that the cheetah possesses. The research was conducted on Kwandwe Private Game Reserve from February 2003 to August 2004. Direct observations, scat analysis and continuous observations were used to avoid bias towards large sized prey found when only using direct observations to study diet. The cheetah killed 15 species and four of the five most important prey species (kudu, springbok, grey duiker and bushbuck) were hunted according to their abundance on the reserve, while impala, the fifth most important prey species, was avoided (i.e. preference index less than one) by the cheetah. The cheetah’s main prey was medium sized ungulates, although the three male coalition killed larger prey than females and females with cubs. The daily food intake per cheetah per day was calculated to range from 5.3kg/cheetah/day for the coalition to 8kg/cheetah/day for a solitary female. Home range areas ranged from 11.1 km² for female cheetah with cubs in a den to 65.6 km² for single female cheetah and core areas ranged from 6 km² for the three male coalition to 26.5 km² for independent cubs. Habitat selection by cheetah on Kwandwe varied between the social groups depending on their susceptibility to predation by lions, their need for cover and need for water, and was similar to what has been previously reported. The home range of the coalition incorporated the most open vegetation type (karroid shrubland) with surrounding denser vegetation, while females occupied areas of denser vegetation. Activity patterns and hunting behaviour varied between different cheetah social groups with female cheetah being more active during day light hours compared to males which made 38% of their kills after dark. The average chase distances for the various cheetah groups varied considerably, and the chase distance for successful hunts was longer than for unsuccessful for all groups except single female cheetah. The percentage of kills’ kleptoparasitised on Kwandwe was very low compared to other studies possibly due to the low density of direct competitors, which in turn lead to longer mean kill retention times. These results suggest that cheetah are more adaptable than previously thought and this adaptability may have important implications for their conservation.
- Full Text:
Gold mineralization in a high grade metamorphic terrane in the Handeni District, Eastern Tanzania
- Bitesigirwe, Godfrey Stephen
- Authors: Bitesigirwe, Godfrey Stephen
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54756 , vital:26609
- Description: Most orogenic type gold deposits are formed under low greenschist facies to mid amphibolite facies metamorphic conditions and deposition is either structurally or lithologically controlled. A few known gold deposits found in high grade metamorphic terranes include those in the Yilgarn craton in Australia, Renco in Zimbabwe, Hemlo in Canada and the recently discovered Handeni deposit in Tanzania. Within Tanzania, gold deposits are mainly hosted in Archaean low grade metamorphic rocks commonly known as the Lake Victoria greenstone belt. The greenstone belts of Tanzania are of Nyanzian age (> 2.5Ga) and are located to the south and east of Lake Victoria on the Tanzania craton. The Tanzania Craton is surrounded by Usagaran 1.9 Ga rocks (the east African orogenic belt (EAO) better known as the Mozambique belt) to the east and the Ubendian belt to the south and west. Published reports show that the eastern part of the Tanzania Craton is dominated by the fragments of Archaean rocks. Metamorphism along East Africa and the Tanzania Craton is due to several geological events. These geological events include the intrusion of granites in the Archaean Tanzania Craton (3 Ga), subduction of ocean plate resulted to the formation of Usagaran belt (1.9 Ga), opening and closure of Mozambique Ocean, which resulted in the formation of the Mozambique belt between 700 – 800 Ma and the Pan African orogeny at 640 – 620 Ma, which is associated with the formation of Gondwana. It is believed that fragments from the Archaean Tanzania craton were re - metamorphosed during these events. The Handeni project (the focus of this thesis) is located in the northern portion of the eastern part of the Usagaran belt (1.9 Ga) comprising the eastern part of Archaean Tanzania Craton. The area is characterized by Proterozic rocks of basaltic composition. The documented 2.7 Ga rocks at the Kilindi Handeni Superterrane at the northern part of the Usagaran belt correlate well with 2.7 Ga of Nyanzian rocks of Archaean Tanzania craton. The Handeni project area is geologically dominated by metamorphosed and deformed units of quartzofeldspathic gneisses, migmatitic gneiss, garnet silicified rock, garnetiferous amphibolite, garnetiferous granulite, graphitic schist and hornblende pyroxenite. Intensive deformation features that were developed include folds (sheath folds, micro and macro scales), faults, shears and regional thrusts. This thesis focuses on identifying the protolith of the rocks, alteration minerals, and metamorphic assemblages in the project area in order to understand the timing of gold mineralization. Geological investigation of core, ore petrology and mineralogy, mineral composition by using JEOL microprobe analysis and XRF analysis of bulk rocks were utilized. All the analytical work was done at the Geology laboratory, Rhodes University. Petrographic analysis shows that the rocks sampled in the study area are characterized by alteration minerals such as calcite, dolomite and sericite. Sulphide minerals including chalcopyrite, pyrrhotite, pyrite, pentlandite and gersdorffite were identified. Gold mineralization is associated with disseminated sulphides in association with trace amounts of base metals. Four rock types were proposed as host rocks for the mineralization, namely garnet silicified rock with superimposed quartz veins, garnetiferous amphibolite, garnetiferous granulite and hornblende pyroxenite. Fold troughs, filled fractures associated with episodes of folding, quartz veins and shear zones are suggested as gold precipitation sites. The presence of high grade metamorphic rocks containing gold, intermediate to low grade assemblages with sulphides and associated hydrothermal alteration as well as a complex deformation history suggests that the Handeni mineralization took place over an extended time period stretching from a ductile to a brittle environment.
- Full Text:
- Authors: Bitesigirwe, Godfrey Stephen
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54756 , vital:26609
- Description: Most orogenic type gold deposits are formed under low greenschist facies to mid amphibolite facies metamorphic conditions and deposition is either structurally or lithologically controlled. A few known gold deposits found in high grade metamorphic terranes include those in the Yilgarn craton in Australia, Renco in Zimbabwe, Hemlo in Canada and the recently discovered Handeni deposit in Tanzania. Within Tanzania, gold deposits are mainly hosted in Archaean low grade metamorphic rocks commonly known as the Lake Victoria greenstone belt. The greenstone belts of Tanzania are of Nyanzian age (> 2.5Ga) and are located to the south and east of Lake Victoria on the Tanzania craton. The Tanzania Craton is surrounded by Usagaran 1.9 Ga rocks (the east African orogenic belt (EAO) better known as the Mozambique belt) to the east and the Ubendian belt to the south and west. Published reports show that the eastern part of the Tanzania Craton is dominated by the fragments of Archaean rocks. Metamorphism along East Africa and the Tanzania Craton is due to several geological events. These geological events include the intrusion of granites in the Archaean Tanzania Craton (3 Ga), subduction of ocean plate resulted to the formation of Usagaran belt (1.9 Ga), opening and closure of Mozambique Ocean, which resulted in the formation of the Mozambique belt between 700 – 800 Ma and the Pan African orogeny at 640 – 620 Ma, which is associated with the formation of Gondwana. It is believed that fragments from the Archaean Tanzania craton were re - metamorphosed during these events. The Handeni project (the focus of this thesis) is located in the northern portion of the eastern part of the Usagaran belt (1.9 Ga) comprising the eastern part of Archaean Tanzania Craton. The area is characterized by Proterozic rocks of basaltic composition. The documented 2.7 Ga rocks at the Kilindi Handeni Superterrane at the northern part of the Usagaran belt correlate well with 2.7 Ga of Nyanzian rocks of Archaean Tanzania craton. The Handeni project area is geologically dominated by metamorphosed and deformed units of quartzofeldspathic gneisses, migmatitic gneiss, garnet silicified rock, garnetiferous amphibolite, garnetiferous granulite, graphitic schist and hornblende pyroxenite. Intensive deformation features that were developed include folds (sheath folds, micro and macro scales), faults, shears and regional thrusts. This thesis focuses on identifying the protolith of the rocks, alteration minerals, and metamorphic assemblages in the project area in order to understand the timing of gold mineralization. Geological investigation of core, ore petrology and mineralogy, mineral composition by using JEOL microprobe analysis and XRF analysis of bulk rocks were utilized. All the analytical work was done at the Geology laboratory, Rhodes University. Petrographic analysis shows that the rocks sampled in the study area are characterized by alteration minerals such as calcite, dolomite and sericite. Sulphide minerals including chalcopyrite, pyrrhotite, pyrite, pentlandite and gersdorffite were identified. Gold mineralization is associated with disseminated sulphides in association with trace amounts of base metals. Four rock types were proposed as host rocks for the mineralization, namely garnet silicified rock with superimposed quartz veins, garnetiferous amphibolite, garnetiferous granulite and hornblende pyroxenite. Fold troughs, filled fractures associated with episodes of folding, quartz veins and shear zones are suggested as gold precipitation sites. The presence of high grade metamorphic rocks containing gold, intermediate to low grade assemblages with sulphides and associated hydrothermal alteration as well as a complex deformation history suggests that the Handeni mineralization took place over an extended time period stretching from a ductile to a brittle environment.
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Ericoid mycorrhizal fungi and potential for inoculation of commercial berry species (Vaccinium corymbosium L.)
- Authors: Bizabani, Christine
- Date: 2011
- Subjects: Ericaceae , Mycorrhizas , Fynbos
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4136 , http://hdl.handle.net/10962/d1016127
- Description: Ericaceous plants are the richest growth form of the fynbos vegetation of South Africa. The fynbos is characterized by highly leached acidic soils, low mineral nutrients and climatically it is a winter rainfall and dry summer region. Ericoid mycorrhizal fungi associate with Erica species enhancing their ability to access essential nutrients for survival under unfavourable growth conditions. The aim of this study was to select local Ericaceae plant species and to isolate, identify and characterize the ericoid endophytes and assess these isolates as potential inocula for commercial berry species. Two ericaceous plants Erica cerinthoides L. and Erica demmissa Klotzsch ex Benth. were identified from the Mountain Drive area of Grahamstown, Eastern Cape. Root staining was used to confirm the mycorrhizal status of both plants. Hyphal coils typical of ericoid association were observed within the epidermal cells of the hair roots under a light microscope. The endophytes were successfully isolated in pure culture on 2% malt extract agar (MEA) and modified Fontana medium. Cultural morphology and microscopy were used for initial identification. Two slow growing isolates were selected. These isolates were further subjected to molecular identification; extracted DNA was amplified using ITS1 and ITS4 fungal primers. The rDNA gene internal transcriber spacer (ITS) was then sequenced and analyzed by comparison to sequences in the GenBank. On the basis of percentage sequence identity Lachnum Retz. species and Cadophora Lagerb. & Melin species were identified as the ericoid endophytes of E. cerinthoides and E. demmissa respectively. The optimum growth parameters of the fungal isolates were determined in 2% MEA incubated at varying temperatures and pH. It was established that both species had optimum growth at 27⁰C and pH 5. The Ericaceae species are sometimes found in metal contaminated sites were ericoid fungi have been proved to alleviate toxicity of their host. The fungal isolates were grown in increasing concentration of Cu²⁺ and Zn²⁺ in 2% MEA. The growth of Lachnum species decreased with increasing Zn²⁺ ions above 2.7 mM while Cadophora species showed a change in morphology and also decreased in growth with increased ion concentration. However there were no significant differences recorded in the growth of Cadophora and Lachnum species on increasing Cu²⁺ concentration. Lachnum and Cadophora isolates were formulated into a semi solid inoculum and inoculated onto micropropagated Vaccinni corymbosum L. plantlets of 5 different varieties. Colonization was low for all varieties, Elliott and Brightwell varieties recorded the highest colonization of 35% and 31% respectively. Lachnum species infected roots showed potential ericoid structures while the Cadophora inoculated plantlets had hyphal coils within the cortical cells typical of ericoid mycorrhizas. Inoculation significantly enhanced the shoot growth of Brightwell and Elliott varieties. The Chandler variety inoculated with Lachnum species showed improved shoot dry weight. The Bluecrop and Elliott varieties inoculated with Cadophora and Lachnum accumulated more root biomass. All inoculated Bluecrop plantlets had an improved canopy growth index. Brightwell plantlets inoculated with Lachnum species also had an enhanced canopy growth index. The growth responses were variable within varieties and between varieties. Treatments with the Cadophora and Lachnum have shown potential in the promotion of growth of the Blueberry species. The findings indicate the need to conduct trials under conditions which simulate the commercial growth conditions so as explore the optimum potential of the isolates.
- Full Text:
- Authors: Bizabani, Christine
- Date: 2011
- Subjects: Ericaceae , Mycorrhizas , Fynbos
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4136 , http://hdl.handle.net/10962/d1016127
- Description: Ericaceous plants are the richest growth form of the fynbos vegetation of South Africa. The fynbos is characterized by highly leached acidic soils, low mineral nutrients and climatically it is a winter rainfall and dry summer region. Ericoid mycorrhizal fungi associate with Erica species enhancing their ability to access essential nutrients for survival under unfavourable growth conditions. The aim of this study was to select local Ericaceae plant species and to isolate, identify and characterize the ericoid endophytes and assess these isolates as potential inocula for commercial berry species. Two ericaceous plants Erica cerinthoides L. and Erica demmissa Klotzsch ex Benth. were identified from the Mountain Drive area of Grahamstown, Eastern Cape. Root staining was used to confirm the mycorrhizal status of both plants. Hyphal coils typical of ericoid association were observed within the epidermal cells of the hair roots under a light microscope. The endophytes were successfully isolated in pure culture on 2% malt extract agar (MEA) and modified Fontana medium. Cultural morphology and microscopy were used for initial identification. Two slow growing isolates were selected. These isolates were further subjected to molecular identification; extracted DNA was amplified using ITS1 and ITS4 fungal primers. The rDNA gene internal transcriber spacer (ITS) was then sequenced and analyzed by comparison to sequences in the GenBank. On the basis of percentage sequence identity Lachnum Retz. species and Cadophora Lagerb. & Melin species were identified as the ericoid endophytes of E. cerinthoides and E. demmissa respectively. The optimum growth parameters of the fungal isolates were determined in 2% MEA incubated at varying temperatures and pH. It was established that both species had optimum growth at 27⁰C and pH 5. The Ericaceae species are sometimes found in metal contaminated sites were ericoid fungi have been proved to alleviate toxicity of their host. The fungal isolates were grown in increasing concentration of Cu²⁺ and Zn²⁺ in 2% MEA. The growth of Lachnum species decreased with increasing Zn²⁺ ions above 2.7 mM while Cadophora species showed a change in morphology and also decreased in growth with increased ion concentration. However there were no significant differences recorded in the growth of Cadophora and Lachnum species on increasing Cu²⁺ concentration. Lachnum and Cadophora isolates were formulated into a semi solid inoculum and inoculated onto micropropagated Vaccinni corymbosum L. plantlets of 5 different varieties. Colonization was low for all varieties, Elliott and Brightwell varieties recorded the highest colonization of 35% and 31% respectively. Lachnum species infected roots showed potential ericoid structures while the Cadophora inoculated plantlets had hyphal coils within the cortical cells typical of ericoid mycorrhizas. Inoculation significantly enhanced the shoot growth of Brightwell and Elliott varieties. The Chandler variety inoculated with Lachnum species showed improved shoot dry weight. The Bluecrop and Elliott varieties inoculated with Cadophora and Lachnum accumulated more root biomass. All inoculated Bluecrop plantlets had an improved canopy growth index. Brightwell plantlets inoculated with Lachnum species also had an enhanced canopy growth index. The growth responses were variable within varieties and between varieties. Treatments with the Cadophora and Lachnum have shown potential in the promotion of growth of the Blueberry species. The findings indicate the need to conduct trials under conditions which simulate the commercial growth conditions so as explore the optimum potential of the isolates.
- Full Text:
Search engine poisoning and its prevalence in modern search engines
- Authors: Blaauw, Pieter
- Date: 2013
- Subjects: Web search engines Internet searching World Wide Web Malware (Computer software) Computer viruses Rootkits (Computer software) Spyware (Computer software)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4572 , http://hdl.handle.net/10962/d1002037
- Description: The prevalence of Search Engine Poisoning in trending topics and popular search terms on the web within search engines is investigated. Search Engine Poisoning is the act of manipulating search engines in order to display search results from websites infected with malware. Research done between February and August 2012, using both manual and automated techniques, shows us how easily the criminal element manages to insert malicious content into web pages related to popular search terms within search engines. In order to provide the reader with a clear overview and understanding of the motives and the methods of the operators of Search Engine Poisoning campaigns, an in-depth review of automated and semi-automated web exploit kits is done, as well as looking into the motives for running these campaigns. Three high profile case studies are examined, and the various Search Engine Poisoning campaigns associated with these case studies are discussed in detail to the reader. From February to August 2012, data was collected from the top trending topics on Google’s search engine along with the top listed sites related to these topics, and then passed through various automated tools to discover if these results have been infiltrated by the operators of Search Engine Poisoning campaings, and the results of these automated scans are then discussed in detail. During the research period, manual searching for Search Engine Poisoning campaigns was also done, using high profile news events and popular search terms. These results are analysed in detail to determine the methods of attack, the purpose of the attack and the parties behind it
- Full Text:
- Authors: Blaauw, Pieter
- Date: 2013
- Subjects: Web search engines Internet searching World Wide Web Malware (Computer software) Computer viruses Rootkits (Computer software) Spyware (Computer software)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4572 , http://hdl.handle.net/10962/d1002037
- Description: The prevalence of Search Engine Poisoning in trending topics and popular search terms on the web within search engines is investigated. Search Engine Poisoning is the act of manipulating search engines in order to display search results from websites infected with malware. Research done between February and August 2012, using both manual and automated techniques, shows us how easily the criminal element manages to insert malicious content into web pages related to popular search terms within search engines. In order to provide the reader with a clear overview and understanding of the motives and the methods of the operators of Search Engine Poisoning campaigns, an in-depth review of automated and semi-automated web exploit kits is done, as well as looking into the motives for running these campaigns. Three high profile case studies are examined, and the various Search Engine Poisoning campaigns associated with these case studies are discussed in detail to the reader. From February to August 2012, data was collected from the top trending topics on Google’s search engine along with the top listed sites related to these topics, and then passed through various automated tools to discover if these results have been infiltrated by the operators of Search Engine Poisoning campaings, and the results of these automated scans are then discussed in detail. During the research period, manual searching for Search Engine Poisoning campaigns was also done, using high profile news events and popular search terms. These results are analysed in detail to determine the methods of attack, the purpose of the attack and the parties behind it
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Software quality assurance in a remote client/contractor context
- Authors: Black, Angus Hugh
- Date: 2006
- Subjects: Computer software -- Quality control , Software engineering , Information technology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4648 , http://hdl.handle.net/10962/d1006615 , Computer software -- Quality control , Software engineering , Information technology
- Description: With the reliance on information technology and the software that this technology utilizes increasing every day, it is of paramount importance that software developed be of an acceptable quality. This quality can be achieved through the utilization of various software engineering standards and guidelines. The question is, to what extent do these standards and guidelines need to be utilized and how are these standards and guidelines implemented? This research focuses on how guidelines developed by standardization bodies and the unified process developed by Rational can be integrated to achieve a suitable process and version control system within the context of a remote client/contractor small team environment.
- Full Text:
- Authors: Black, Angus Hugh
- Date: 2006
- Subjects: Computer software -- Quality control , Software engineering , Information technology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4648 , http://hdl.handle.net/10962/d1006615 , Computer software -- Quality control , Software engineering , Information technology
- Description: With the reliance on information technology and the software that this technology utilizes increasing every day, it is of paramount importance that software developed be of an acceptable quality. This quality can be achieved through the utilization of various software engineering standards and guidelines. The question is, to what extent do these standards and guidelines need to be utilized and how are these standards and guidelines implemented? This research focuses on how guidelines developed by standardization bodies and the unified process developed by Rational can be integrated to achieve a suitable process and version control system within the context of a remote client/contractor small team environment.
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Cleavage of the precursor coat protein of black beetle virus strain w17 in rabbit reticulocyte lysate
- Authors: Blackhurst, Diane Mary
- Date: 1988
- Subjects: Beetles , Insects -- Viruses
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3880 , http://hdl.handle.net/10962/d1001614
- Description: Black beetle virus (BBV) is a bipartite single-stranded RNA virus belonging to the family Nodaviridae. Its host range has been found to be limited to insects. RNA 1, the larger of the two RNA molecules, with a MW of 1,15 x 10⁶ and the smaller RNA 2 with a MW of 0,46 x 10⁶, are both packaged in the same virus particle. The two RNA molecules are translated separately, with RNA 1 coding for protein A of MW 105 x 10³ and RNA 2 coding for protein α of MW 47 x 10³. Protein α is the major capsid protein precursor, which during in vivo maturation is cleaved to form the coat protein β of MW 43 x 10³, and protein γ of MW 5 x 10³. Cell-free translation of BBV (strain W17) mRNA was carried out in rabbit reticulocyte lysates. Protein α was detectable between 0 and 30 minutes after RNA addition. A protein 'β', which was found to co-electrophorese on polyacrylamide gels with authentic β and which was immunoprecipitated by anti-BBV antiserum, was detectable after 30 minutes. Results of this work show that the formation of 'β' could be prevented by the addition of RNase to the lysate, indicating that intact RNA is necessary for α to β cleavage. Arresting protein synthesis by the addition of cycloheximide to the lysate mix did not inhibit the cleavage. The formation of β could also be prevented by cooling the lysate mix to 1°C. Cleavage of α to β still occurred when RNA 2, without the presence of RNA 1, was translated. Therefore the cleavage is not dependent on a translation product of RNA 1. Sedimentation of lysate on sucrose density gradients showed that α to β cleavage was not accompanied by assembly of BBV RNA and protein lnto a viral substructure as has been shown to occur with some viruses, for example certain picornaviruses. Serial dilution of lysate containing α showed that the level of β decreased with increasing dilution, indicating that the cleavage is not mediated by autocatalysis, but by some other unknown factor. Although much work has been carried out on black beetle virus, no work has been published to date concerning α to β cleavage as an indication of assembly in rabbit reticulocyte lysates. Results of these cell-free translation experiments thus indicate that BBV coat protein precursor α, in association with its messenger RNA 2, undergoes a maturation cleavage in the lysate to produce BBV coat protein β. In addition, this cleavage seems to occur without assembly into any intermediate viral structure
- Full Text:
- Authors: Blackhurst, Diane Mary
- Date: 1988
- Subjects: Beetles , Insects -- Viruses
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3880 , http://hdl.handle.net/10962/d1001614
- Description: Black beetle virus (BBV) is a bipartite single-stranded RNA virus belonging to the family Nodaviridae. Its host range has been found to be limited to insects. RNA 1, the larger of the two RNA molecules, with a MW of 1,15 x 10⁶ and the smaller RNA 2 with a MW of 0,46 x 10⁶, are both packaged in the same virus particle. The two RNA molecules are translated separately, with RNA 1 coding for protein A of MW 105 x 10³ and RNA 2 coding for protein α of MW 47 x 10³. Protein α is the major capsid protein precursor, which during in vivo maturation is cleaved to form the coat protein β of MW 43 x 10³, and protein γ of MW 5 x 10³. Cell-free translation of BBV (strain W17) mRNA was carried out in rabbit reticulocyte lysates. Protein α was detectable between 0 and 30 minutes after RNA addition. A protein 'β', which was found to co-electrophorese on polyacrylamide gels with authentic β and which was immunoprecipitated by anti-BBV antiserum, was detectable after 30 minutes. Results of this work show that the formation of 'β' could be prevented by the addition of RNase to the lysate, indicating that intact RNA is necessary for α to β cleavage. Arresting protein synthesis by the addition of cycloheximide to the lysate mix did not inhibit the cleavage. The formation of β could also be prevented by cooling the lysate mix to 1°C. Cleavage of α to β still occurred when RNA 2, without the presence of RNA 1, was translated. Therefore the cleavage is not dependent on a translation product of RNA 1. Sedimentation of lysate on sucrose density gradients showed that α to β cleavage was not accompanied by assembly of BBV RNA and protein lnto a viral substructure as has been shown to occur with some viruses, for example certain picornaviruses. Serial dilution of lysate containing α showed that the level of β decreased with increasing dilution, indicating that the cleavage is not mediated by autocatalysis, but by some other unknown factor. Although much work has been carried out on black beetle virus, no work has been published to date concerning α to β cleavage as an indication of assembly in rabbit reticulocyte lysates. Results of these cell-free translation experiments thus indicate that BBV coat protein precursor α, in association with its messenger RNA 2, undergoes a maturation cleavage in the lysate to produce BBV coat protein β. In addition, this cleavage seems to occur without assembly into any intermediate viral structure
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The ichthyofauna and piscivorous avifauna in a small temporarily open/closed Eastern Cape estuary, South Africa
- Authors: Blake, Justin David
- Date: 2010
- Subjects: Estuarine fishes -- South Africa -- Eastern Cape , Bird populations -- South Africa -- Riet River Estuary , Estuaries -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Riet River Estuary , Estuarine animals -- South Africa -- Riet River Estuary , Birds -- South Africa -- Riet River Estuary , Riet River Estuary (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5709 , http://hdl.handle.net/10962/d1005395 , Estuarine fishes -- South Africa -- Eastern Cape , Bird populations -- South Africa -- Riet River Estuary , Estuaries -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Riet River Estuary , Estuarine animals -- South Africa -- Riet River Estuary , Birds -- South Africa -- Riet River Estuary , Riet River Estuary (South Africa)
- Description: The spatial and temporal patterns in selected components of the ichthyofauna and piscivorous avifauna in the small temporarily open/closed Riet River Estuary located on the eastern seaboard of southern Africa was investigated monthly over the period August 2005 to July 2006. The ichthyofauna within the littoral zone of the estuary was sampled using a 5 m seine net (8 stations) while a 30 m seine net (4 stations) was employed to sample the fish in the channel. Bird counts were made along repeat transects along the length of the estuary. Total ichthyofaunal abundances and biomass ranged between 1.60 and 8.67 individuals m⁻² and 0.45 to 21.76 g wwt m⁻² within the littoral zone, and between 0.08 and 0.44 individuals m⁻² and 0.58 and 36.52 g wwt m⁻² in the channel of the estuary. The highest values were generally recorded during the summer months. Results of the numerical analysis indicated that the breaching events recorded over the study period did not lead to a common trend in the ichthyofaunal community. In the absence of a link to the marine environment, the ichthyofaunal community in the littoral zone was numerically dominated by the estuarine resident species, Gilchristella aestuaria and to a lesser extent by Glossogobius callidus, which collectively accounted for ca. 54% of the total ichthyofauna sampled. The establishment of a link to the marine environment coincided with increased numbers of marine breeding species including Atherina breviceps and Rhabdosargus holubi to total fish counts within the estuary. Hierarchical cluster analysis did not identify any spatial patterns in the community structure of the ichthyofauna in the littoral zone or channel zone of the estuary, which could likely be linked to the absence of any distinct horizontal patterns in salinity and temperature within the system. A total of thirteen piscivorous bird species was recorded over the study period. Of the recorded species, six species were wading piscivores, four species were aerial divers and the remaining three species were pursuit swimmers. There were no significant correlations between the estimates of the ichthyofaunal abundance and biomass and bird numbers evident during the study (P> 0.05 in both cases). The Reed Cormorant (Phalacrocorax africanus) was the dominant species throughout the study, with a mean of 8.25 (SD ± 7.90) individuals per count. Mean values of the Pied Kingfisher (Ceryle rudis) and Giant Kingfisher (Megaceryle maximus) were 3.42 (SD ± 1.20) and 1.17 (SD ± 0.60) individuals per count, respectively. The remaining species revealed mean values < 0.5 individuals per count. The highest bird numbers were recorded in winter reflecting the migration of large numbers of the Reed Cormorant into the system. Breaching events were associated with a decrease in total bird numbers, which was most likely due to loss of potential foraging habitat (littoral zone) for waders resulting from reduced water levels. Monthly food consumption by all piscivorous birds revealed large temporal variability, ranging from 26.35 to 140.58 kg per month. The observed variability could be linked to mouth phase and bird numbers.
- Full Text:
- Authors: Blake, Justin David
- Date: 2010
- Subjects: Estuarine fishes -- South Africa -- Eastern Cape , Bird populations -- South Africa -- Riet River Estuary , Estuaries -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Riet River Estuary , Estuarine animals -- South Africa -- Riet River Estuary , Birds -- South Africa -- Riet River Estuary , Riet River Estuary (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5709 , http://hdl.handle.net/10962/d1005395 , Estuarine fishes -- South Africa -- Eastern Cape , Bird populations -- South Africa -- Riet River Estuary , Estuaries -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Riet River Estuary , Estuarine animals -- South Africa -- Riet River Estuary , Birds -- South Africa -- Riet River Estuary , Riet River Estuary (South Africa)
- Description: The spatial and temporal patterns in selected components of the ichthyofauna and piscivorous avifauna in the small temporarily open/closed Riet River Estuary located on the eastern seaboard of southern Africa was investigated monthly over the period August 2005 to July 2006. The ichthyofauna within the littoral zone of the estuary was sampled using a 5 m seine net (8 stations) while a 30 m seine net (4 stations) was employed to sample the fish in the channel. Bird counts were made along repeat transects along the length of the estuary. Total ichthyofaunal abundances and biomass ranged between 1.60 and 8.67 individuals m⁻² and 0.45 to 21.76 g wwt m⁻² within the littoral zone, and between 0.08 and 0.44 individuals m⁻² and 0.58 and 36.52 g wwt m⁻² in the channel of the estuary. The highest values were generally recorded during the summer months. Results of the numerical analysis indicated that the breaching events recorded over the study period did not lead to a common trend in the ichthyofaunal community. In the absence of a link to the marine environment, the ichthyofaunal community in the littoral zone was numerically dominated by the estuarine resident species, Gilchristella aestuaria and to a lesser extent by Glossogobius callidus, which collectively accounted for ca. 54% of the total ichthyofauna sampled. The establishment of a link to the marine environment coincided with increased numbers of marine breeding species including Atherina breviceps and Rhabdosargus holubi to total fish counts within the estuary. Hierarchical cluster analysis did not identify any spatial patterns in the community structure of the ichthyofauna in the littoral zone or channel zone of the estuary, which could likely be linked to the absence of any distinct horizontal patterns in salinity and temperature within the system. A total of thirteen piscivorous bird species was recorded over the study period. Of the recorded species, six species were wading piscivores, four species were aerial divers and the remaining three species were pursuit swimmers. There were no significant correlations between the estimates of the ichthyofaunal abundance and biomass and bird numbers evident during the study (P> 0.05 in both cases). The Reed Cormorant (Phalacrocorax africanus) was the dominant species throughout the study, with a mean of 8.25 (SD ± 7.90) individuals per count. Mean values of the Pied Kingfisher (Ceryle rudis) and Giant Kingfisher (Megaceryle maximus) were 3.42 (SD ± 1.20) and 1.17 (SD ± 0.60) individuals per count, respectively. The remaining species revealed mean values < 0.5 individuals per count. The highest bird numbers were recorded in winter reflecting the migration of large numbers of the Reed Cormorant into the system. Breaching events were associated with a decrease in total bird numbers, which was most likely due to loss of potential foraging habitat (littoral zone) for waders resulting from reduced water levels. Monthly food consumption by all piscivorous birds revealed large temporal variability, ranging from 26.35 to 140.58 kg per month. The observed variability could be linked to mouth phase and bird numbers.
- Full Text:
The geology and evaluation of the "A"-reef at No.3 shaft, Western Holdings Mine, Welkom goldfield
- Blamey, Nigel John Frederick
- Authors: Blamey, Nigel John Frederick
- Date: 1992
- Subjects: Gold mines and mining -- South Africa -- Free State , Gold ores -- Geology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4957 , http://hdl.handle.net/10962/d1005569 , Gold mines and mining -- South Africa -- Free State , Gold ores -- Geology -- South Africa
- Description: The "A" -Reef occurs within the Aandenk Formation of the Central Rand Group, Witwatersrand Supergroup, in the Welkom Goldfield. It comprises the Witpan and Uitsig Reefs which are both oligomictic conglomerates, and are exploited for their gold content by Anglo American Corporation. The main Witpan channel complex is orientated in a NW-SE direction and occurs close to No.3 Shaft of Western Holdings Mine where it is currently being mined. The Witpan Reef varies in thickness from 7-220cm, with lateral facies changes controlling the thickness. Within the reef, gold is associated with degradation surfaces, carbonaceous material, increase in pebble sphericity, and channel edges. Two channel edges have yielded the best gold values on No.3 Shaft although the potential for further payable gold lies in the recognition of sieve conditions. The potential also exists for extensions of the "carbon"-bearing Uitsig channel currently being mined on President Steyn Mine. The palaeo-environment proposed for formation of the Witpan Reef is a braidplain that was partly reworked by a brief transgression. Ore evaluation using geostatistics was considered a valid technique as the dataset is sufficiently well structured. Semi-variograms in the channel and across-channel directions differ markedly. It was found that variograms of gold in cmg/t lacked sufficient structure for modelling, however, log semi-variogram modelling followed by simple log-kriging and back-transformation, proved to be the most successful method. Owing to the morphology and distribution of gold within the reef, a geologically based geostatistical valuation method is proposed. The potential for further exploration of "A" -Reef depends on a substantially higher gold price. In this event, exploration of Uitsig Reef to the southwest of the current mining area is recommended as well as a new exploration strategy for Witpan Reef.
- Full Text:
- Authors: Blamey, Nigel John Frederick
- Date: 1992
- Subjects: Gold mines and mining -- South Africa -- Free State , Gold ores -- Geology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4957 , http://hdl.handle.net/10962/d1005569 , Gold mines and mining -- South Africa -- Free State , Gold ores -- Geology -- South Africa
- Description: The "A" -Reef occurs within the Aandenk Formation of the Central Rand Group, Witwatersrand Supergroup, in the Welkom Goldfield. It comprises the Witpan and Uitsig Reefs which are both oligomictic conglomerates, and are exploited for their gold content by Anglo American Corporation. The main Witpan channel complex is orientated in a NW-SE direction and occurs close to No.3 Shaft of Western Holdings Mine where it is currently being mined. The Witpan Reef varies in thickness from 7-220cm, with lateral facies changes controlling the thickness. Within the reef, gold is associated with degradation surfaces, carbonaceous material, increase in pebble sphericity, and channel edges. Two channel edges have yielded the best gold values on No.3 Shaft although the potential for further payable gold lies in the recognition of sieve conditions. The potential also exists for extensions of the "carbon"-bearing Uitsig channel currently being mined on President Steyn Mine. The palaeo-environment proposed for formation of the Witpan Reef is a braidplain that was partly reworked by a brief transgression. Ore evaluation using geostatistics was considered a valid technique as the dataset is sufficiently well structured. Semi-variograms in the channel and across-channel directions differ markedly. It was found that variograms of gold in cmg/t lacked sufficient structure for modelling, however, log semi-variogram modelling followed by simple log-kriging and back-transformation, proved to be the most successful method. Owing to the morphology and distribution of gold within the reef, a geologically based geostatistical valuation method is proposed. The potential for further exploration of "A" -Reef depends on a substantially higher gold price. In this event, exploration of Uitsig Reef to the southwest of the current mining area is recommended as well as a new exploration strategy for Witpan Reef.
- Full Text:
MEQSILHOUETTE: a mm-VLBI observation and signal corruption simulator
- Authors: Blecher, Tariq
- Date: 2017
- Subjects: Large astronomical telescopes , Very long baseline interferometry , MEQSILHOUETTE (Software) , Event horizon telescope
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/40713 , vital:25019
- Description: The Event Horizon Telescope (EHT) aims to resolve the innermost emission of nearby supermassive black holes, Sgr A* and M87, on event horizon scales. This emission is predicted to be gravitationally lensed by the black hole which should produce a shadow (or silhouette) feature, a precise measurement of which is a test of gravity in the strong-field regime. This emission is also an ideal probe of the innermost accretion and jet-launch physics, offering the new insights into this data-limited observing regime. The EHT will use the technique of Very Long Baseline Interferometry (VLBI) at (sub)millimetre wavelengths, which has a diffraction limited angular resolution of order ~ 10 µ-arcsec. However, this technique suffers from unique challenges, including scattering and attenuation in the troposphere and interstellar medium; variable source structure; as well as antenna pointing errors comparable to the size of the primary beam. In this thesis, we present the meqsilhouette software package which is focused towards simulating realistic EHT data. It has the capability to simulate a time-variable source, and includes realistic descriptions of the effects of the troposphere, the interstellar medium as well as primary beams and associated antenna pointing errors. We have demonstrated through several examples simulations that these effects can limit the ability to measure the key science parameters. This simulator can be used to research calibration, parameter estimation and imaging strategies, as well as gain insight into possible systematic uncertainties.
- Full Text:
- Authors: Blecher, Tariq
- Date: 2017
- Subjects: Large astronomical telescopes , Very long baseline interferometry , MEQSILHOUETTE (Software) , Event horizon telescope
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/40713 , vital:25019
- Description: The Event Horizon Telescope (EHT) aims to resolve the innermost emission of nearby supermassive black holes, Sgr A* and M87, on event horizon scales. This emission is predicted to be gravitationally lensed by the black hole which should produce a shadow (or silhouette) feature, a precise measurement of which is a test of gravity in the strong-field regime. This emission is also an ideal probe of the innermost accretion and jet-launch physics, offering the new insights into this data-limited observing regime. The EHT will use the technique of Very Long Baseline Interferometry (VLBI) at (sub)millimetre wavelengths, which has a diffraction limited angular resolution of order ~ 10 µ-arcsec. However, this technique suffers from unique challenges, including scattering and attenuation in the troposphere and interstellar medium; variable source structure; as well as antenna pointing errors comparable to the size of the primary beam. In this thesis, we present the meqsilhouette software package which is focused towards simulating realistic EHT data. It has the capability to simulate a time-variable source, and includes realistic descriptions of the effects of the troposphere, the interstellar medium as well as primary beams and associated antenna pointing errors. We have demonstrated through several examples simulations that these effects can limit the ability to measure the key science parameters. This simulator can be used to research calibration, parameter estimation and imaging strategies, as well as gain insight into possible systematic uncertainties.
- Full Text:
Clays derived from the Lower Dwyka Shales, occurring in the vicinity of Grahamstown
- Authors: Blignaut, J J G
- Date: 1928
- Subjects: Clay -- South Africa -- Grahamstown Clay -- Economic aspects -- South Africa -- Grahamstown Geology -- South Africa -- Grahamstown Mineralogy -- South Africa -- Grahamstown
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4935 , http://hdl.handle.net/10962/d1005547
- Description: The Lower Dwyka Shales in South Africa oocur south of latitude 32⁰; resting conformably upon the Witteberg quartzites, which extend as coastal mountain rangee along the length of the South Coast. The shales, being less resistant to weathering than the quartzites below, and the Dwyka Tillite immediately above, are consequently found in valleys between these two formations. In one of these valleys Grahamstown is situated; where the Lower Dwyka Shales have been eroded by the various rivulets forming the eource of the Blaauwkrantz River. A geological map of the country round Grahamstown was prepared in eome detail, showing all the various rock formations occurring in the area, the full extent of the Lower Dwyka Shales being mapped with the Dwyka Tillite and the Witteberg Quartzite along its margins. The map includes an area of about six miles by three, with the Cathedral spire occupying an approximately central position, and is prepared on a scale of 5.65 inches to one mile. Traversing was done by means of prismatic compass and pacing.
- Full Text:
- Authors: Blignaut, J J G
- Date: 1928
- Subjects: Clay -- South Africa -- Grahamstown Clay -- Economic aspects -- South Africa -- Grahamstown Geology -- South Africa -- Grahamstown Mineralogy -- South Africa -- Grahamstown
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4935 , http://hdl.handle.net/10962/d1005547
- Description: The Lower Dwyka Shales in South Africa oocur south of latitude 32⁰; resting conformably upon the Witteberg quartzites, which extend as coastal mountain rangee along the length of the South Coast. The shales, being less resistant to weathering than the quartzites below, and the Dwyka Tillite immediately above, are consequently found in valleys between these two formations. In one of these valleys Grahamstown is situated; where the Lower Dwyka Shales have been eroded by the various rivulets forming the eource of the Blaauwkrantz River. A geological map of the country round Grahamstown was prepared in eome detail, showing all the various rock formations occurring in the area, the full extent of the Lower Dwyka Shales being mapped with the Dwyka Tillite and the Witteberg Quartzite along its margins. The map includes an area of about six miles by three, with the Cathedral spire occupying an approximately central position, and is prepared on a scale of 5.65 inches to one mile. Traversing was done by means of prismatic compass and pacing.
- Full Text:
The effect of water temperature on the distribution of the Eastern Cape redfin minnow, Pseudobarbus afer (Peters, 1864)
- Authors: Bloy, Lesley Elizabeth
- Date: 2018
- Subjects: Minnows -- South Africa -- Eastern Cape , Cyprinidae -- South Africa -- Eastern Cape , Minnows -- Effect of temperature on -- South Africa -- Eastern Cape , Minnows -- Effect of exotic animals on -- South Africa -- Eastern Cape , Introduced organisms -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62017 , vital:28096
- Description: The main objective of this MSc study was to disentangle whether temperature or the presence of non-native fish was limiting the distribution of Pseudobarbus afer (Peters, 1864) in the Blindekloof stream, Eastern Cape, South Africa. The aims were to: i) describe the thermal regime of the Blindekloof stream; ii) conduct experiments to determine the preferred and critical temperatures of P. afer and; iii) use snorkel surveys to contextualise the distribution of both native and non-native fishes in the Blindekloof stream to assess whether the downstream distribution of P. afer was likely to be influenced by temperature or by the presence of non-native, predatory fishes. To describe the thermal regime, year-long temperature data from four long term monitoring sites in the Blindekloof stream were collected using Hobo temperature loggers and analysed in order to better understand the thermal profile, the thermal variation and the rate of temperature change in the stream. The warmest temperatures were recorded in late December 2015 (absolute maximum of 29.4 °C). The coolest water temperatures were recorded in early August 2015 (absolute minimum of 9.5 °C). There is both seasonal and diel variation in temperature with mean, minimum, maximum, 7 day mean, 7 day maximum and temperature ranges differing significantly between sites. With knowledge of the thermal regime of a monitored reach of the Blindekloof stream, the thermal tolerance and preference of P. afer were investigated. The thermal tolerance of P. afer was investigated using the Critical Thermal Method (CTM) which uses non-lethal endpoints (the loss of equilibrium). At low acclimatization temperatures (11.9 ± 0.7 °C), the mean CTmax of P. afer was found to be 29.9 ± 0.7 °C, while at a higher acclimatization temperature (19.9 ± 0.1 °C), the mean CTmax was 35.1 ± 0.6°C. Custom-built thermal choice tanks were used to investigate the thermal preference of P. afer in both summer and winter. The preferred median temperatures for the summer experiments ranged from 22.4 - 29.3 °C while the winter preferred median temperatures ranged from 18.5 - 23.1 °C. The thermal tolerance of P. afer was compared to the thermal regime of the stream and the results suggest that temperature is not limiting the distribution of P. afer. Snorkel surveys were used to determine the distribution of fishes in the Blindekloof stream. Analysis of the distribution data suggests that, in the absence of non-native predatory species, native fishes have the potential to inhabit pools throughout the system right to the confluence, thus predatory fishes and not temperature was limiting P. afer distributions.
- Full Text:
- Authors: Bloy, Lesley Elizabeth
- Date: 2018
- Subjects: Minnows -- South Africa -- Eastern Cape , Cyprinidae -- South Africa -- Eastern Cape , Minnows -- Effect of temperature on -- South Africa -- Eastern Cape , Minnows -- Effect of exotic animals on -- South Africa -- Eastern Cape , Introduced organisms -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62017 , vital:28096
- Description: The main objective of this MSc study was to disentangle whether temperature or the presence of non-native fish was limiting the distribution of Pseudobarbus afer (Peters, 1864) in the Blindekloof stream, Eastern Cape, South Africa. The aims were to: i) describe the thermal regime of the Blindekloof stream; ii) conduct experiments to determine the preferred and critical temperatures of P. afer and; iii) use snorkel surveys to contextualise the distribution of both native and non-native fishes in the Blindekloof stream to assess whether the downstream distribution of P. afer was likely to be influenced by temperature or by the presence of non-native, predatory fishes. To describe the thermal regime, year-long temperature data from four long term monitoring sites in the Blindekloof stream were collected using Hobo temperature loggers and analysed in order to better understand the thermal profile, the thermal variation and the rate of temperature change in the stream. The warmest temperatures were recorded in late December 2015 (absolute maximum of 29.4 °C). The coolest water temperatures were recorded in early August 2015 (absolute minimum of 9.5 °C). There is both seasonal and diel variation in temperature with mean, minimum, maximum, 7 day mean, 7 day maximum and temperature ranges differing significantly between sites. With knowledge of the thermal regime of a monitored reach of the Blindekloof stream, the thermal tolerance and preference of P. afer were investigated. The thermal tolerance of P. afer was investigated using the Critical Thermal Method (CTM) which uses non-lethal endpoints (the loss of equilibrium). At low acclimatization temperatures (11.9 ± 0.7 °C), the mean CTmax of P. afer was found to be 29.9 ± 0.7 °C, while at a higher acclimatization temperature (19.9 ± 0.1 °C), the mean CTmax was 35.1 ± 0.6°C. Custom-built thermal choice tanks were used to investigate the thermal preference of P. afer in both summer and winter. The preferred median temperatures for the summer experiments ranged from 22.4 - 29.3 °C while the winter preferred median temperatures ranged from 18.5 - 23.1 °C. The thermal tolerance of P. afer was compared to the thermal regime of the stream and the results suggest that temperature is not limiting the distribution of P. afer. Snorkel surveys were used to determine the distribution of fishes in the Blindekloof stream. Analysis of the distribution data suggests that, in the absence of non-native predatory species, native fishes have the potential to inhabit pools throughout the system right to the confluence, thus predatory fishes and not temperature was limiting P. afer distributions.
- Full Text:
In silico characterization of plasmodial transketolases as potential malaria drug target
- Authors: Boateng, Rita Afriyie
- Date: 2018
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63540 , vital:28433
- Description: Expected release date-April 2019
- Full Text:
- Authors: Boateng, Rita Afriyie
- Date: 2018
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63540 , vital:28433
- Description: Expected release date-April 2019
- Full Text:
Developing a form-process framework to describe the functioning of semi-arid alluvial fans in the Baviaanskloof Valley, South Africa
- Authors: Bobbins, Kerry Leigh
- Date: 2012
- Subjects: Alluvial fans -- Research -- South Africa Sedimentation and deposition -- Research -- South Africa Geomorphology -- Research -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4825 , http://hdl.handle.net/10962/d1005500
- Description: The Baviaanskloof catchment is a semi-arid catchment located in the Cape Fold Mountains of South Africa. Little is known about the functioning of the complicated Baviaanskloof fluvial system and the role alluvial fans in the fluvial landscape. This thesis will contribute to field of geomorphology and, more specifically, to the field of fan morphometry by producing a standalone fan framework outlining methods to investigate the influence of external and internal control variables on alluvial fans. In this thesis, outcomes of the applied framework and case study are used to develop fan restoration guidelines for the Baviaanskloof Valley. The framework incorporates external and internal fan control variables at a valley-wide and local fan scale. External control variables include accommodation space, base-level change, and drainage basin inputs. Internal control variables include fan style, morphometry and fan channels. In order to apply the framework, fan morphometry data was required. This data was collected by creating a spatial plan of fans and basins in the valley. Outcomes of the applied framework include; an understanding of baselevel change on fans, relationships between fan basin characteristics and the fan surface and insight into fan channel processes. Results of the applied framework are investigated further using bivariate (correlation matrix) and multivariate (principle component analysis and regression analysis) analysis techniques. Significant relationships identified are: drainage basin area versus fan area, fan area and fan slope and drainage basin ruggedness and basin size. The primary outcomes of this thesis include an alluvial fan form-process framework, key considerations to be included in alluvial fan restoration projects and fan restoration guidelines. Contributions of this thesis to broader alluvial fan morphology science includes new insights into general fan literature by compiling a form-process alluvial fan classification framework to identify external and internal fan control variables and identify fan form. Additions have been made to Clarke’s (2010) evolutionary stages to describe stages 4 and 5 of fan evolution that has been adapted to describe fan evolution and differentiate between stages of mature fan evolution. This thesis has also contributed to the study of alluvial fans in South Africa, particularly in the Baviaanskloof Valley. The layout of the procedural guidelines and key considerations for an alluvial fan project provides a guide for rapid fan assessment for maximum cost and time benefits for stakeholders.
- Full Text:
- Authors: Bobbins, Kerry Leigh
- Date: 2012
- Subjects: Alluvial fans -- Research -- South Africa Sedimentation and deposition -- Research -- South Africa Geomorphology -- Research -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4825 , http://hdl.handle.net/10962/d1005500
- Description: The Baviaanskloof catchment is a semi-arid catchment located in the Cape Fold Mountains of South Africa. Little is known about the functioning of the complicated Baviaanskloof fluvial system and the role alluvial fans in the fluvial landscape. This thesis will contribute to field of geomorphology and, more specifically, to the field of fan morphometry by producing a standalone fan framework outlining methods to investigate the influence of external and internal control variables on alluvial fans. In this thesis, outcomes of the applied framework and case study are used to develop fan restoration guidelines for the Baviaanskloof Valley. The framework incorporates external and internal fan control variables at a valley-wide and local fan scale. External control variables include accommodation space, base-level change, and drainage basin inputs. Internal control variables include fan style, morphometry and fan channels. In order to apply the framework, fan morphometry data was required. This data was collected by creating a spatial plan of fans and basins in the valley. Outcomes of the applied framework include; an understanding of baselevel change on fans, relationships between fan basin characteristics and the fan surface and insight into fan channel processes. Results of the applied framework are investigated further using bivariate (correlation matrix) and multivariate (principle component analysis and regression analysis) analysis techniques. Significant relationships identified are: drainage basin area versus fan area, fan area and fan slope and drainage basin ruggedness and basin size. The primary outcomes of this thesis include an alluvial fan form-process framework, key considerations to be included in alluvial fan restoration projects and fan restoration guidelines. Contributions of this thesis to broader alluvial fan morphology science includes new insights into general fan literature by compiling a form-process alluvial fan classification framework to identify external and internal fan control variables and identify fan form. Additions have been made to Clarke’s (2010) evolutionary stages to describe stages 4 and 5 of fan evolution that has been adapted to describe fan evolution and differentiate between stages of mature fan evolution. This thesis has also contributed to the study of alluvial fans in South Africa, particularly in the Baviaanskloof Valley. The layout of the procedural guidelines and key considerations for an alluvial fan project provides a guide for rapid fan assessment for maximum cost and time benefits for stakeholders.
- Full Text:
The metallogeny of the Upington and Kenhardt area, northern Cape
- Authors: Boelema, Robert
- Date: 1995
- Subjects: Metallogeny -- South Africa , Metallogeny -- South Africa -- Northern Cape , Plate tectonics -- South Africa -- Northern Cape , Geology, Structural -- South Africa -- Northern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4975 , http://hdl.handle.net/10962/d1005587 , Metallogeny -- South Africa , Metallogeny -- South Africa -- Northern Cape , Plate tectonics -- South Africa -- Northern Cape , Geology, Structural -- South Africa -- Northern Cape
- Description: In the Upington region, there are three major- tectonic crustal provinces; namely the Kaapvaal Craton, Kheis and Namaqua tectonic provinces. The Eburnian-aged (early Proterozoic) Kheis Province developed along the western flank of the Archaean Kaapvaal Craton while the Kibaran-aged (middle Proterozoic) Namaqua Metamorphic Province, superimposed on the Eburnian-aged basement, developed to the east of the Kheis Province. The Namaqua Metamorphic Province is divided into the Gordonia and Bushmanland Subprovinces, the former being further subdivided into various tectonostratigraphic terranes. These are termed, from west to east, the Kakamas, Areachap, and Upington Terranes. The Upington Terrane includes fault bounded grabens with accompanied bimodal volcanism and sedimentation of the Wilgenhoutsdrif and Koras Groups. The Areachap Terrane consists predominantly of amphibolites generated in an island arc environment while the Kakamas Terrane is characterised by volcano-sedimentary sequences which have been extensively intruded by syn to late-tectonic predominantly I-type Keimoes Suite granitoids. The main styles of mineralisation correlate well with the various tectonostratigraphic terranes. Sedimentary exhalative massive sulphide deposits are characteristic of the Bushmanland Subprovince and are thought to be associated with the deposits at Aggeneys and Putsberg to the west of the area under investigation. These deposits are considered to have been deposited in an east-west-elongated intracontinental basin. The Kakamas Terrane is typified by granite-related mineralisation. In the eastern portion of the Kakamas Terrane, Sn-Wand base metal-bearing veins occur while pegmatites are developed in the western portion. These two styles of granite-related mineralisation is considered to reflect differing depths of formation due mainly to varying degrees of thrusting. The Areachap Terrane consists of volcanogenic massive sulphide deposits of the Besshi-type and is considered to have formed in a back-arc environment. In the Upington Terrane, the Wilgenhoutsdrif and Koras Groups consists essentially of minor Cu occurrences mainly disseminated within basalts and in structural trap sites. The possibility for sediment-hosted Cu deposits is not ruled out. More recent surface processes have led to uranium and gypsum deposits in pans, river beds and calcretes. Eburnian aged tectonic setting remains enigmatic. Kibaran-aged tectonics which best fits the metallogeny of the area under investigation is considered to be of a subduction zone from west to east formed by the collision of the Bushmanland "microcontinent" against the Kaapvaal Craton. Subduction fbrmed an island arc setting in which the massive sulphide deposits were formed in the Areachap Terrane while the Wilgenhoutsdrif Groups developed in a marginal basin. Further convergence led to collision of the two continents and underriding of the Bushmanland "microcontinent" which generated predominantly I-type granitoids represented by the Keimoes Suite. The level of emplacement of these granitoids is a reflection of the degree of foreland thrusting and produced shallower level Sn-W and base metal vein-type mineralisation closer to the suture zone and deeper level pegmatites further from the suture zone to the west. The final period of deformation is represented by northward lateral movement which created "pull apart" fault-bounded basins into which the Koras Group was deposited.
- Full Text:
- Authors: Boelema, Robert
- Date: 1995
- Subjects: Metallogeny -- South Africa , Metallogeny -- South Africa -- Northern Cape , Plate tectonics -- South Africa -- Northern Cape , Geology, Structural -- South Africa -- Northern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4975 , http://hdl.handle.net/10962/d1005587 , Metallogeny -- South Africa , Metallogeny -- South Africa -- Northern Cape , Plate tectonics -- South Africa -- Northern Cape , Geology, Structural -- South Africa -- Northern Cape
- Description: In the Upington region, there are three major- tectonic crustal provinces; namely the Kaapvaal Craton, Kheis and Namaqua tectonic provinces. The Eburnian-aged (early Proterozoic) Kheis Province developed along the western flank of the Archaean Kaapvaal Craton while the Kibaran-aged (middle Proterozoic) Namaqua Metamorphic Province, superimposed on the Eburnian-aged basement, developed to the east of the Kheis Province. The Namaqua Metamorphic Province is divided into the Gordonia and Bushmanland Subprovinces, the former being further subdivided into various tectonostratigraphic terranes. These are termed, from west to east, the Kakamas, Areachap, and Upington Terranes. The Upington Terrane includes fault bounded grabens with accompanied bimodal volcanism and sedimentation of the Wilgenhoutsdrif and Koras Groups. The Areachap Terrane consists predominantly of amphibolites generated in an island arc environment while the Kakamas Terrane is characterised by volcano-sedimentary sequences which have been extensively intruded by syn to late-tectonic predominantly I-type Keimoes Suite granitoids. The main styles of mineralisation correlate well with the various tectonostratigraphic terranes. Sedimentary exhalative massive sulphide deposits are characteristic of the Bushmanland Subprovince and are thought to be associated with the deposits at Aggeneys and Putsberg to the west of the area under investigation. These deposits are considered to have been deposited in an east-west-elongated intracontinental basin. The Kakamas Terrane is typified by granite-related mineralisation. In the eastern portion of the Kakamas Terrane, Sn-Wand base metal-bearing veins occur while pegmatites are developed in the western portion. These two styles of granite-related mineralisation is considered to reflect differing depths of formation due mainly to varying degrees of thrusting. The Areachap Terrane consists of volcanogenic massive sulphide deposits of the Besshi-type and is considered to have formed in a back-arc environment. In the Upington Terrane, the Wilgenhoutsdrif and Koras Groups consists essentially of minor Cu occurrences mainly disseminated within basalts and in structural trap sites. The possibility for sediment-hosted Cu deposits is not ruled out. More recent surface processes have led to uranium and gypsum deposits in pans, river beds and calcretes. Eburnian aged tectonic setting remains enigmatic. Kibaran-aged tectonics which best fits the metallogeny of the area under investigation is considered to be of a subduction zone from west to east formed by the collision of the Bushmanland "microcontinent" against the Kaapvaal Craton. Subduction fbrmed an island arc setting in which the massive sulphide deposits were formed in the Areachap Terrane while the Wilgenhoutsdrif Groups developed in a marginal basin. Further convergence led to collision of the two continents and underriding of the Bushmanland "microcontinent" which generated predominantly I-type granitoids represented by the Keimoes Suite. The level of emplacement of these granitoids is a reflection of the degree of foreland thrusting and produced shallower level Sn-W and base metal vein-type mineralisation closer to the suture zone and deeper level pegmatites further from the suture zone to the west. The final period of deformation is represented by northward lateral movement which created "pull apart" fault-bounded basins into which the Koras Group was deposited.
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Profiling for volatile compounds in the Kgalagadi desert truffle
- Authors: Bogopa, Juda
- Date: 2013
- Subjects: Desert plants -- Botswana -- Kgalagadi District Supercritical fluid extraction Gas chromatography Mass spectrometry Tuberaceae -- Analysis -- Botswana -- Kgalagadi District Truffles -- Analysis -- Botswana -- Kgalagadi District
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4280 , http://hdl.handle.net/10962/d1002006
- Description: The thesis focuses on ‘green’ sample preparation techniques that utilise minimal or no organic solvents thereby producing negligible volumes of organic waste, to ensure safety both to user and the environment. Volatile compounds were extracted and pre-concentrated from Kgalagadi desert truffles (kalaharituber) by headspace solid phase microextraction (HSSPME) and supercritical fluid extraction (SFE). PHWE was employed for the extraction of amino acids and fatty acids. Subsequent analysis of volatile compounds was carried out by gas chromatography coupled with mass spectrometry. Four types of HS-SPME fibers (PDMS 100 μm, PDMS 7 μm, Polyacrylate 85 μm, CAR/DVB/PDMS 50/30 μm) were evaluated. A total of 24 volatile compounds with a molecular weight range from 110 to 354, the most prominent peak being 2-t-Butyl-2,3-dimethyl-3-buten-1-ol (C₁₀H₂₀O, MW 156) were detected after sampling with a PDMS 100 μm fiber. Less volatile compounds were detected after SFE with CO2. A total of 16 amino acids were identified while 17 fatty acids (MW from 132.12 to 367.49) were also identified. The characteristic profile of the Kgalagadi desert truffle was found to contain mainly fatty acid methyl esters and unsaturated aliphatic hydrocarbons. The most prominent compound peaks identified were; 2-t-butyl-2,3-dimethyl- 3-buten-1-ol, disulfide, ethyl benzoic acid 2-4-dhydroxy-3,6-dimethyl-methyl ester, 8,11- octadecanoic acid methyl ester, benzoic acid, 2,4-dihydroxy-3,6-dimethyl-methyl ester, isoquinoline, 1 butyl-3,4-dihydro and 3-heptanone, 6 methyl. Optimization results indicated that fresh slices from the heart of truffles were the best to use for HS-SPME-GCMS volatile compound analysis as they showed a higher sensitivity
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- Authors: Bogopa, Juda
- Date: 2013
- Subjects: Desert plants -- Botswana -- Kgalagadi District Supercritical fluid extraction Gas chromatography Mass spectrometry Tuberaceae -- Analysis -- Botswana -- Kgalagadi District Truffles -- Analysis -- Botswana -- Kgalagadi District
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4280 , http://hdl.handle.net/10962/d1002006
- Description: The thesis focuses on ‘green’ sample preparation techniques that utilise minimal or no organic solvents thereby producing negligible volumes of organic waste, to ensure safety both to user and the environment. Volatile compounds were extracted and pre-concentrated from Kgalagadi desert truffles (kalaharituber) by headspace solid phase microextraction (HSSPME) and supercritical fluid extraction (SFE). PHWE was employed for the extraction of amino acids and fatty acids. Subsequent analysis of volatile compounds was carried out by gas chromatography coupled with mass spectrometry. Four types of HS-SPME fibers (PDMS 100 μm, PDMS 7 μm, Polyacrylate 85 μm, CAR/DVB/PDMS 50/30 μm) were evaluated. A total of 24 volatile compounds with a molecular weight range from 110 to 354, the most prominent peak being 2-t-Butyl-2,3-dimethyl-3-buten-1-ol (C₁₀H₂₀O, MW 156) were detected after sampling with a PDMS 100 μm fiber. Less volatile compounds were detected after SFE with CO2. A total of 16 amino acids were identified while 17 fatty acids (MW from 132.12 to 367.49) were also identified. The characteristic profile of the Kgalagadi desert truffle was found to contain mainly fatty acid methyl esters and unsaturated aliphatic hydrocarbons. The most prominent compound peaks identified were; 2-t-butyl-2,3-dimethyl- 3-buten-1-ol, disulfide, ethyl benzoic acid 2-4-dhydroxy-3,6-dimethyl-methyl ester, 8,11- octadecanoic acid methyl ester, benzoic acid, 2,4-dihydroxy-3,6-dimethyl-methyl ester, isoquinoline, 1 butyl-3,4-dihydro and 3-heptanone, 6 methyl. Optimization results indicated that fresh slices from the heart of truffles were the best to use for HS-SPME-GCMS volatile compound analysis as they showed a higher sensitivity
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Immunolocalisation of, and ultrastructural changes in the LH gonadotropes of Miniopterus schreibersii and Rhinolophus capensis (Mammalia: Chiroptera) in relation to their reproductive cycles
- Authors: Bojarski, Christina
- Date: 1990
- Subjects: Bats -- Reproduction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5589 , http://hdl.handle.net/10962/d1002039
- Description: LH gonadotropes are successfully identified in the anterior pituitary of Minioptems schreibersii and Rhinolophus capensis, using immunogold labelling at the light and electron microscopy level. The gonadotropes are oval to polygonal in shape and possess numerous small secretory granules, which contain LH throughout the year. Their distribution is similar in both species, although the abundance varies slightly between species and sexes. Seasonal changes are detected in gonadotrope ultrastructure, pituitary LH levels, and plasma hormone levels, and activity pattern of LH gonadotropes generally coincide with reproductive activity in both bat species. In female M. schreibersii gonadotrope activity, pituitary LH, and plasma LH levels coincide with development, implantation and gestation. During the delayed implantation gonadotropes are inactive and and high follicular period of plasma LH levels are low, coinciding with corpus luteum inactivity and low plasma progesterone levels. Implantation coincides with increased activity of the gonadotrope activity, increased plasma LH levels, reactivation of the corpus luteum and elevated plasma progesterone levels. Activation of LH gonadotropes towards the end of hibernation may be initiated by the winter solstice, which marks the change to increasing daylength. In female R capensis gonadotrope activity and high pituitary and plasma LH levels occur towards the end of follicular development. During hibernation gonadotrope activity and plasma LH levels decrease. Ovulation coincides with increasing gonadotrope activity (which follows the winter solstice), although a preovulatory peak in plasma LH is not detected. In male M. schreibersii and R. capensis, gonadotrope activity and high plasma LH and testosterone levels coincide with spermatogenesis, except during a period of reproductive inactivity in spring in male M. schreibersii, where gonadotropes appear active and plasma LH and testosterone levels are high. The reason for this apparent activity is not known. Male sperm storage during hibernation in male R. capensis coincides with low gonadotrope activity and low plasma LH and testosterone levels. Factors initiating gonadotrope activity and hence spermatogenesis are probably decreasing daylength (summer solstice) in M. schreibersii and increasing ambient temperatures and food abundance (following the hibernation period) in R.capensis.
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- Authors: Bojarski, Christina
- Date: 1990
- Subjects: Bats -- Reproduction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5589 , http://hdl.handle.net/10962/d1002039
- Description: LH gonadotropes are successfully identified in the anterior pituitary of Minioptems schreibersii and Rhinolophus capensis, using immunogold labelling at the light and electron microscopy level. The gonadotropes are oval to polygonal in shape and possess numerous small secretory granules, which contain LH throughout the year. Their distribution is similar in both species, although the abundance varies slightly between species and sexes. Seasonal changes are detected in gonadotrope ultrastructure, pituitary LH levels, and plasma hormone levels, and activity pattern of LH gonadotropes generally coincide with reproductive activity in both bat species. In female M. schreibersii gonadotrope activity, pituitary LH, and plasma LH levels coincide with development, implantation and gestation. During the delayed implantation gonadotropes are inactive and and high follicular period of plasma LH levels are low, coinciding with corpus luteum inactivity and low plasma progesterone levels. Implantation coincides with increased activity of the gonadotrope activity, increased plasma LH levels, reactivation of the corpus luteum and elevated plasma progesterone levels. Activation of LH gonadotropes towards the end of hibernation may be initiated by the winter solstice, which marks the change to increasing daylength. In female R capensis gonadotrope activity and high pituitary and plasma LH levels occur towards the end of follicular development. During hibernation gonadotrope activity and plasma LH levels decrease. Ovulation coincides with increasing gonadotrope activity (which follows the winter solstice), although a preovulatory peak in plasma LH is not detected. In male M. schreibersii and R. capensis, gonadotrope activity and high plasma LH and testosterone levels coincide with spermatogenesis, except during a period of reproductive inactivity in spring in male M. schreibersii, where gonadotropes appear active and plasma LH and testosterone levels are high. The reason for this apparent activity is not known. Male sperm storage during hibernation in male R. capensis coincides with low gonadotrope activity and low plasma LH and testosterone levels. Factors initiating gonadotrope activity and hence spermatogenesis are probably decreasing daylength (summer solstice) in M. schreibersii and increasing ambient temperatures and food abundance (following the hibernation period) in R.capensis.
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