The scope of environmental protection under the legal framework of the World Trade Organisation : an evaluation of the issues and implications for developing countries
- Authors: Umenze, Nnamdi Stanislaus
- Date: 2018
- Subjects: Environmental protection -- Developing countries Environmental law -- Developing countries
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: http://hdl.handle.net/10353/10802 , vital:35762
- Description: Over the years, the extent to which the legal framework of the World Trade Organisation (WTO) makes provision for environmental protection has been a major issue within the organisation. While trade liberalisation and environmental protection are fundamental objectives of the WTO to be pursued in line with the sustainable development goals as enshrined in the Preamble of the Marrakesh Agreement, the organisation does not have a specific agreement on the environment. Moreover, efforts by the member states of the WTO to reform the environmental protection regime of the global trading system have achieved little. Nevertheless, under the current legal framework of the WTO, members are allowed, subject to a number of conditions, to adopt trade-related measures aimed at protecting the environment. Employing documentary research methodology, this study critically evaluates the relationship between trade and the environment, the provisions made for environmental protection in selected WTO agreements and the extent to which trade-related environmental protection measures are permissible under the legal framework of the WTO. It also evaluates the implications of unilateral and the Multilateral Environmental Agreements (MEA)-based environmental trade measures as well as the possibility of a clash of policy objectives between the WTO Agreements and the MEAs, given that some of the MEAs contain environmental trade measures prohibited by the WTO free trade rules. The findings made in this study suggest that states generally prefer to enforce unilateral environmental trade measures against foreign goods. This has allowed states, in some instances, to hide under the guise of environmental protection to pursue protectionist’s interests, eco-imperialism, etc., leading to the trade and environment-related disputes at the WTO. Moreover, the WTO is made up of member states that are at different levels of development and possess different environmental protection standards. Hence, there is concern from developing countries, on the one hand, that their economic interests are being frustrated by the stringent application of unilateral environmental trade measures in the developed countries, and, on the other hand, that the environmental burden of international trade is being shifted to the developing countries as the developed countries tighten their environmental standards. The study concludes that the environmental protection regime of the WTO as it stands is not adequate to guarantee the balance of trade and environmental protection objectives as well as the balance of interests between the developed and developing countries. The study, therefore, recommends reform in the environmental protection regime of the WTO to ensure that the environmental protection measures are adequately provided for and that balance of interests between the developed and developing countries is maintained in order to ensure sustainable global trade. It further recommends that a mutually reinforcing relationship should be established between the WTO and the MEAs to avoid a possible clash of policy objectives.
- Full Text:
- Date Issued: 2018
- Authors: Umenze, Nnamdi Stanislaus
- Date: 2018
- Subjects: Environmental protection -- Developing countries Environmental law -- Developing countries
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: http://hdl.handle.net/10353/10802 , vital:35762
- Description: Over the years, the extent to which the legal framework of the World Trade Organisation (WTO) makes provision for environmental protection has been a major issue within the organisation. While trade liberalisation and environmental protection are fundamental objectives of the WTO to be pursued in line with the sustainable development goals as enshrined in the Preamble of the Marrakesh Agreement, the organisation does not have a specific agreement on the environment. Moreover, efforts by the member states of the WTO to reform the environmental protection regime of the global trading system have achieved little. Nevertheless, under the current legal framework of the WTO, members are allowed, subject to a number of conditions, to adopt trade-related measures aimed at protecting the environment. Employing documentary research methodology, this study critically evaluates the relationship between trade and the environment, the provisions made for environmental protection in selected WTO agreements and the extent to which trade-related environmental protection measures are permissible under the legal framework of the WTO. It also evaluates the implications of unilateral and the Multilateral Environmental Agreements (MEA)-based environmental trade measures as well as the possibility of a clash of policy objectives between the WTO Agreements and the MEAs, given that some of the MEAs contain environmental trade measures prohibited by the WTO free trade rules. The findings made in this study suggest that states generally prefer to enforce unilateral environmental trade measures against foreign goods. This has allowed states, in some instances, to hide under the guise of environmental protection to pursue protectionist’s interests, eco-imperialism, etc., leading to the trade and environment-related disputes at the WTO. Moreover, the WTO is made up of member states that are at different levels of development and possess different environmental protection standards. Hence, there is concern from developing countries, on the one hand, that their economic interests are being frustrated by the stringent application of unilateral environmental trade measures in the developed countries, and, on the other hand, that the environmental burden of international trade is being shifted to the developing countries as the developed countries tighten their environmental standards. The study concludes that the environmental protection regime of the WTO as it stands is not adequate to guarantee the balance of trade and environmental protection objectives as well as the balance of interests between the developed and developing countries. The study, therefore, recommends reform in the environmental protection regime of the WTO to ensure that the environmental protection measures are adequately provided for and that balance of interests between the developed and developing countries is maintained in order to ensure sustainable global trade. It further recommends that a mutually reinforcing relationship should be established between the WTO and the MEAs to avoid a possible clash of policy objectives.
- Full Text:
- Date Issued: 2018
Exploring the standardization of musical and dancing styles in relation to worship styles in the Church of Central Africa Presbyterian, Blantyre Synod, Malawi
- Authors: University of Fort Hare
- Date: 2018
- Subjects: Presbyterian Church -- Malawi Presbyterians -- Malawi Church music -- Malawi
- Language: English
- Type: Thesis , Masters , Music
- Identifier: http://hdl.handle.net/10353/10442 , vital:35468
- Description: The study sought to explore the standardization of music and dance styles in relation to worship styles in the CCAP Blantyre Synod in Malawi. The study further sought to understand the factors that influence the selection of songs during worship, and strategies for standardizing the existing songs in the church. The research was guided by the qualitative research approach. The interpretive research paradigm guided the interpretation of data in this study. A case study research design was used to get an in- depth understanding of the issues related to music and dance in the CCAP. The participants were purposively selected and interviews were conducted. Focus group interviews and observation supplemented the data collected through one on one interviews. Data analysis was achieved through a detailed narration of the participants‟ responses and, thereafter, themes were identified for further discussion. The main findings of the study were: factors influencing selection of music during worship services; lack or availability of music selection guidelines; age of choir members; commercialization and instrumentation of music; the use of traditional and classical music in the church; mixed dancing styles being introduced in the church; and the suggestion to use the Bible as framework for resolving conflicts in the church; and the need to train church members on church music. The study recommends the use of detailed and appropriate music guidelines in selecting songs. In cases of conflicting tastes in music, the study has proposed a process of resolving such conflicts within the CCAP of Blantyre Synod. The study also examined music and dance styles but not on other aspects of worship such as prayer and offering. The findings of this study may be useful in advising and resolving challenges associated with the incorporation of music in the church.
- Full Text:
- Date Issued: 2018
- Authors: University of Fort Hare
- Date: 2018
- Subjects: Presbyterian Church -- Malawi Presbyterians -- Malawi Church music -- Malawi
- Language: English
- Type: Thesis , Masters , Music
- Identifier: http://hdl.handle.net/10353/10442 , vital:35468
- Description: The study sought to explore the standardization of music and dance styles in relation to worship styles in the CCAP Blantyre Synod in Malawi. The study further sought to understand the factors that influence the selection of songs during worship, and strategies for standardizing the existing songs in the church. The research was guided by the qualitative research approach. The interpretive research paradigm guided the interpretation of data in this study. A case study research design was used to get an in- depth understanding of the issues related to music and dance in the CCAP. The participants were purposively selected and interviews were conducted. Focus group interviews and observation supplemented the data collected through one on one interviews. Data analysis was achieved through a detailed narration of the participants‟ responses and, thereafter, themes were identified for further discussion. The main findings of the study were: factors influencing selection of music during worship services; lack or availability of music selection guidelines; age of choir members; commercialization and instrumentation of music; the use of traditional and classical music in the church; mixed dancing styles being introduced in the church; and the suggestion to use the Bible as framework for resolving conflicts in the church; and the need to train church members on church music. The study recommends the use of detailed and appropriate music guidelines in selecting songs. In cases of conflicting tastes in music, the study has proposed a process of resolving such conflicts within the CCAP of Blantyre Synod. The study also examined music and dance styles but not on other aspects of worship such as prayer and offering. The findings of this study may be useful in advising and resolving challenges associated with the incorporation of music in the church.
- Full Text:
- Date Issued: 2018
Laccase production by selected bacteria species isolated from some aquatic and terrestrial milieu of the Eastern Cape : applications in wastewater treatment
- Authors: Unuofin, John Onolame
- Date: 2018
- Subjects: Laccase Sewage -- Purification -- Biological treatment Water -- Purification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9626 , vital:34810
- Description: Aromatic pollutants are a diverse group of chemicals which are continuously produced from industrialization, urbanization and sophistication in technological advancement. Pristine water source polluted by these chemicls makes the water unsafe for human consumption, and as well disrupts the trophic structure of the aquatic milieu. Physico-chemical treatment techniques employed so far have been accompanied by major drawbacks which have overriden the relative successes recorded, hence, greener, simpler and more efficient methods of pollutant transformation is imperative. The prospect of enzymatic treatment of pollutants has gradually been receiving growing attention in contemporary times due to the their environmental friendliness and production economic feasiblity. Laccase, a multicopper oxidase has heightened its appeal towards environmental and biotechnological applications due to its broad substrate specificity and its requirement of atmospheric molecular oxygen as a cosubstrate and the discharge of water as the byproduct. Hence, this present study was designed to evaluate the biotechnological potentials of laccases produced by some bacteria species from some aquatic biomes of the Eastern Cape Province, South Africa. The laccase-producing bacteria were isolated from selected environmental samples by selective enrichment using selective aromatic compounds as sole carbon source and subsequently, laccase-screening phenolic substrates. The laccase-producing bacteria were identified by molecular techniques as proteobacteria belonging to the following genera: Achromobacter, Bordetella, Citrobacter, Pseudomonas and Stenotrophomonas. Optimisation of laccase production in a submerged fermentation was by traditional and statistical methods, where four isolates (Hb9c; Achromobacter xylosoxidans HWN16, Hb16c; Bordetella bronchisepta HSO16, Berl11b2; Stenotrophomonas maltophilia BIJ16, Ie1c; Citrobacter freundii LLJ16) were evaluated for the fermentative production of laccase from lignocellulosic agroindustrial residues. Predictions from statistical optimisation showed that weakly acidic conditions (pH 5) and low agitation speed (100 rpm) were required for maximum laccase production from mandarin peelings (0.5 g/200 mL) and NaNO3 (0.25 g/200 mL) in Hb9c, maize stover (0.50 g/200 mL) and NaNO3 (0.050 g/200mL) in Berl11b2 while a lower agitation speed (50 rpm) was required for maximum laccase output from 2.0 g/200 mL maize stover and 0.050 g/200 mL KNO3 in Ie1c. However, 2.50 g/200 mL wheat bran, 0.050 g/200 mL yeast extract and 50 rpm agitiation under acidic conditions (pH3) yielded maximum laccase titres in Hb16c. Further characterisation of Hb9c and Ie1c laccase secretions portrayed their polyextremotolerant capacities. They were active at a broad range of tempertaure (0-90 degreesC); with optima at 70°C (Hb9c) and 60°C (Ie1c), pH (3-11); with optima at pH 6 (Ie1c) and pH 8 (Hb9c), respectively, and were equally thermo- and pH-stable. Their activities were either improved or left unabated by high concentrations of cations, detergents, and chloride. In addition, catalytic activities of Hb9c and Ie1c laccase secretions increased when they were preincubated with 2 – 20 percent of fluoride, a potent inhibitor. Consequently, a molecular perspective depicted the isolates to have multiple homologous laccase encoding genes. The enzymes were successfully immobilised on solid supports comprising gelatin and Na-alginate with a recovery of cca. 85 percent residual activity after 8 cycles of oprertional stability experiments. The immobilised laccases were remarkable in the decolourisation of synthetic dyes, albeit, free forms also elicited satisfactory performances. Ultimately, the application of free laccases in denim bleaching, individually or with a blend of a mediator, ABTS, showed that denim colours could be bleached without the need for chemical bleaching agents. The results obtained suggest the bacteria laccases produced from lignocellulosic wastes may serve as potent degraders of phenolic pollutants in water and, may also contribute to the bioeconomy and promote greener techniques for industrial applications.
- Full Text:
- Date Issued: 2018
- Authors: Unuofin, John Onolame
- Date: 2018
- Subjects: Laccase Sewage -- Purification -- Biological treatment Water -- Purification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9626 , vital:34810
- Description: Aromatic pollutants are a diverse group of chemicals which are continuously produced from industrialization, urbanization and sophistication in technological advancement. Pristine water source polluted by these chemicls makes the water unsafe for human consumption, and as well disrupts the trophic structure of the aquatic milieu. Physico-chemical treatment techniques employed so far have been accompanied by major drawbacks which have overriden the relative successes recorded, hence, greener, simpler and more efficient methods of pollutant transformation is imperative. The prospect of enzymatic treatment of pollutants has gradually been receiving growing attention in contemporary times due to the their environmental friendliness and production economic feasiblity. Laccase, a multicopper oxidase has heightened its appeal towards environmental and biotechnological applications due to its broad substrate specificity and its requirement of atmospheric molecular oxygen as a cosubstrate and the discharge of water as the byproduct. Hence, this present study was designed to evaluate the biotechnological potentials of laccases produced by some bacteria species from some aquatic biomes of the Eastern Cape Province, South Africa. The laccase-producing bacteria were isolated from selected environmental samples by selective enrichment using selective aromatic compounds as sole carbon source and subsequently, laccase-screening phenolic substrates. The laccase-producing bacteria were identified by molecular techniques as proteobacteria belonging to the following genera: Achromobacter, Bordetella, Citrobacter, Pseudomonas and Stenotrophomonas. Optimisation of laccase production in a submerged fermentation was by traditional and statistical methods, where four isolates (Hb9c; Achromobacter xylosoxidans HWN16, Hb16c; Bordetella bronchisepta HSO16, Berl11b2; Stenotrophomonas maltophilia BIJ16, Ie1c; Citrobacter freundii LLJ16) were evaluated for the fermentative production of laccase from lignocellulosic agroindustrial residues. Predictions from statistical optimisation showed that weakly acidic conditions (pH 5) and low agitation speed (100 rpm) were required for maximum laccase production from mandarin peelings (0.5 g/200 mL) and NaNO3 (0.25 g/200 mL) in Hb9c, maize stover (0.50 g/200 mL) and NaNO3 (0.050 g/200mL) in Berl11b2 while a lower agitation speed (50 rpm) was required for maximum laccase output from 2.0 g/200 mL maize stover and 0.050 g/200 mL KNO3 in Ie1c. However, 2.50 g/200 mL wheat bran, 0.050 g/200 mL yeast extract and 50 rpm agitiation under acidic conditions (pH3) yielded maximum laccase titres in Hb16c. Further characterisation of Hb9c and Ie1c laccase secretions portrayed their polyextremotolerant capacities. They were active at a broad range of tempertaure (0-90 degreesC); with optima at 70°C (Hb9c) and 60°C (Ie1c), pH (3-11); with optima at pH 6 (Ie1c) and pH 8 (Hb9c), respectively, and were equally thermo- and pH-stable. Their activities were either improved or left unabated by high concentrations of cations, detergents, and chloride. In addition, catalytic activities of Hb9c and Ie1c laccase secretions increased when they were preincubated with 2 – 20 percent of fluoride, a potent inhibitor. Consequently, a molecular perspective depicted the isolates to have multiple homologous laccase encoding genes. The enzymes were successfully immobilised on solid supports comprising gelatin and Na-alginate with a recovery of cca. 85 percent residual activity after 8 cycles of oprertional stability experiments. The immobilised laccases were remarkable in the decolourisation of synthetic dyes, albeit, free forms also elicited satisfactory performances. Ultimately, the application of free laccases in denim bleaching, individually or with a blend of a mediator, ABTS, showed that denim colours could be bleached without the need for chemical bleaching agents. The results obtained suggest the bacteria laccases produced from lignocellulosic wastes may serve as potent degraders of phenolic pollutants in water and, may also contribute to the bioeconomy and promote greener techniques for industrial applications.
- Full Text:
- Date Issued: 2018
The generation and characterisation of neutralising antibodies against the Theiler’s murine encephalomyelitis virus (TMEV) GDVII capsid reveals the potential binding site of the host cell co-receptor, heparan sulfate:
- Upfold, Nicole, Ross, Caroline J, Tastan Bishop, Özlem, Luke, Garry A, Knox, Caroline M
- Authors: Upfold, Nicole , Ross, Caroline J , Tastan Bishop, Özlem , Luke, Garry A , Knox, Caroline M
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148181 , vital:38717 , DOI: 10.1016/j.virusres.2017.11.017
- Description: The early stages of picornavirus capsid assembly and the host factors involved are poorly understood. Since the localisation of viral proteins in infected cells can provide information on their function, antibodies against purified Theiler's murine encephalomyelitis virus (TMEV) GDVII capsids were generated by immunisation of rabbits. The resultant anti-TMEV capsid antibodies recognised a C-terminal region of VP1 but not VP2 or VP3 by Western analysis. Examination of the sites of TMEV capsid assembly by indirect immunofluorescence and confocal microscopy showed that at 5 h post infection, capsid signal was diffusely cytoplasmic with strong perinuclear staining and moved into large punctate structures from 6 to 8 h post infection.
- Full Text:
- Date Issued: 2018
- Authors: Upfold, Nicole , Ross, Caroline J , Tastan Bishop, Özlem , Luke, Garry A , Knox, Caroline M
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148181 , vital:38717 , DOI: 10.1016/j.virusres.2017.11.017
- Description: The early stages of picornavirus capsid assembly and the host factors involved are poorly understood. Since the localisation of viral proteins in infected cells can provide information on their function, antibodies against purified Theiler's murine encephalomyelitis virus (TMEV) GDVII capsids were generated by immunisation of rabbits. The resultant anti-TMEV capsid antibodies recognised a C-terminal region of VP1 but not VP2 or VP3 by Western analysis. Examination of the sites of TMEV capsid assembly by indirect immunofluorescence and confocal microscopy showed that at 5 h post infection, capsid signal was diffusely cytoplasmic with strong perinuclear staining and moved into large punctate structures from 6 to 8 h post infection.
- Full Text:
- Date Issued: 2018
Interaction of catechol O-methyltransferase with gold and silver nanoparticles
- Authors: Usman, Aminu
- Date: 2018
- Subjects: Parkinson's disease , Methyltransferases , Catechol , Nanoparticles
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/61818 , vital:28063 , DOI https://doi.org/10.21504/10962/61818
- Description: Catechol O-methyltransferase (S-adenosyl-Z-methionine: catechol O-methyltransferase; COMT; EC 2.1.1.6) is a ubiquitous enzyme that catalyses the transfer of a methyl group from the cofactor, S-adenosyl-Z-methionine (SAM) to a hydroxyl group of endogenous and exogenous catechol-containing moieties. The physiological role of this enzyme is the methylation and thereby inactivation of the catechol-containing bio-active and bio-toxic compounds, including catechol-neurotransmitters, catechol-estrogens and catechol-containing drugs. Activity of this enzyme is implicated in the treatment of Parkinson’s disease and is associated with other diseases including breast cancer and an array neuropsychological disorders, such as schizophrenia. This thesis explores the use of gold and silver nanoparticles (NPs) (AuNPs and AgNPs) to inhibit the catalytic activity of mammalian COMT. Because of its accessibility and availability, we initially investigated bovine soluble COMT (BSCOMT) from liver tissue. Bioinformatic analyses and structural modeling revealed high (>90%) sequence similarity between BSCOMT and human soluble COMT (HSCOMT). BSCOMT was partially purified to 7.78 fold, 1.65% yield and had a specific activity of 0.052 U/mg. It had pH and temperature optima of 8.5 and 40oC, respectively. The Km, Vmax, Kcat and Kcat/Km towards esculetin methylation were respectively 1.475±0.130 pM, 0.0353±0.001 pmol/ml/min, 1.748 x 10-2±5.0x10-4 min-1 and 1.18x10-2 M-1. min-1. HSCOMT was expressed in Escherichia coli BL21(DE3) which showed optimal activity for esculetin methylation at pH and temperature of 7.0 and 30°C, respectively. It was purified to 5.62 fold, 22.6% yield with a specific activity of 3.85 U/mg. HSCOMT kinetic plots, upon incubation of the reaction mixture at 30°C for 5 min before addition of SAM was hyperbolic with Km, Vmax, Kcat and Kcat/Km values of 1.79 pM, 0.412 pmol/ml/min, 2.08 min-1 and 1.165 M-1. min-1, respectively. AuNPs and AgNPs showed a concentration dependent inhibition of HSCOMT activity upon increasing the 5 min incubation time to 1 h. Interestingly, HSCOMT kinetics, with 1 h incubation at 30°C, showed a sigmoidal curve, as well as increased activity. Incubation of the reaction mixture in the presence of 60 pM AuNPs and/or AgNPs for 1 hreversed the observed sigmoidal to a hyperbolic curve, with kinetic parameters comparable to those of 5 min incubation. SDS-PAGE analyses of HSCOMT after the kinetic experiments showed the enzyme incubated for 5 min as a monomer, while that which was incubated for 1 h migrated substantially as dimer. However, the HSCOMT incubated for 1 h in the presence of 60 pM AuNPs and/or AgNPs migrated as a monomer. This indicated that the extension of the incubation period allowed the dimerization of HSCOMT, which exhibited sigmoidal kinetics and higher activity. The presence of NPs impeded the HSCOMT dimerization which decreased the activity. Varying the concentration of SAM suggested that SAM had an allosteric modulatory effect on HSCOMT. Absorption spectroscopy indicated adsorption of HSCOMT on the gold and silver NP surfaces and the formation of NPs-HSCOMT corona. Fluorescence spectroscopy showed that the interaction of HSCOMT with both gold and silver NPs was governed by a static quenching mechanism, implying the formation of a non-fluorescent fluorophore-NP complex at the ground state. Further fluorometric analyses indicated that both gold and silver NPs had contact with Trp143; that the interactions were spontaneous and were driven by electrostatic interactions. Fourier transform infrared spectroscopic studies showed the adsorption of HSCOMT of the NPs surfaces to cause relaxation of the enzyme’s B-sheet structures. Molecular docking studies indicated involvement of largely hydrophilic amino acids, with the interacting distances of less than 3.5A. These findings signify the potential of nanotechnology in the control of COMT catalytic activity for the management of the COMT-related disorders. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
- Authors: Usman, Aminu
- Date: 2018
- Subjects: Parkinson's disease , Methyltransferases , Catechol , Nanoparticles
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/61818 , vital:28063 , DOI https://doi.org/10.21504/10962/61818
- Description: Catechol O-methyltransferase (S-adenosyl-Z-methionine: catechol O-methyltransferase; COMT; EC 2.1.1.6) is a ubiquitous enzyme that catalyses the transfer of a methyl group from the cofactor, S-adenosyl-Z-methionine (SAM) to a hydroxyl group of endogenous and exogenous catechol-containing moieties. The physiological role of this enzyme is the methylation and thereby inactivation of the catechol-containing bio-active and bio-toxic compounds, including catechol-neurotransmitters, catechol-estrogens and catechol-containing drugs. Activity of this enzyme is implicated in the treatment of Parkinson’s disease and is associated with other diseases including breast cancer and an array neuropsychological disorders, such as schizophrenia. This thesis explores the use of gold and silver nanoparticles (NPs) (AuNPs and AgNPs) to inhibit the catalytic activity of mammalian COMT. Because of its accessibility and availability, we initially investigated bovine soluble COMT (BSCOMT) from liver tissue. Bioinformatic analyses and structural modeling revealed high (>90%) sequence similarity between BSCOMT and human soluble COMT (HSCOMT). BSCOMT was partially purified to 7.78 fold, 1.65% yield and had a specific activity of 0.052 U/mg. It had pH and temperature optima of 8.5 and 40oC, respectively. The Km, Vmax, Kcat and Kcat/Km towards esculetin methylation were respectively 1.475±0.130 pM, 0.0353±0.001 pmol/ml/min, 1.748 x 10-2±5.0x10-4 min-1 and 1.18x10-2 M-1. min-1. HSCOMT was expressed in Escherichia coli BL21(DE3) which showed optimal activity for esculetin methylation at pH and temperature of 7.0 and 30°C, respectively. It was purified to 5.62 fold, 22.6% yield with a specific activity of 3.85 U/mg. HSCOMT kinetic plots, upon incubation of the reaction mixture at 30°C for 5 min before addition of SAM was hyperbolic with Km, Vmax, Kcat and Kcat/Km values of 1.79 pM, 0.412 pmol/ml/min, 2.08 min-1 and 1.165 M-1. min-1, respectively. AuNPs and AgNPs showed a concentration dependent inhibition of HSCOMT activity upon increasing the 5 min incubation time to 1 h. Interestingly, HSCOMT kinetics, with 1 h incubation at 30°C, showed a sigmoidal curve, as well as increased activity. Incubation of the reaction mixture in the presence of 60 pM AuNPs and/or AgNPs for 1 hreversed the observed sigmoidal to a hyperbolic curve, with kinetic parameters comparable to those of 5 min incubation. SDS-PAGE analyses of HSCOMT after the kinetic experiments showed the enzyme incubated for 5 min as a monomer, while that which was incubated for 1 h migrated substantially as dimer. However, the HSCOMT incubated for 1 h in the presence of 60 pM AuNPs and/or AgNPs migrated as a monomer. This indicated that the extension of the incubation period allowed the dimerization of HSCOMT, which exhibited sigmoidal kinetics and higher activity. The presence of NPs impeded the HSCOMT dimerization which decreased the activity. Varying the concentration of SAM suggested that SAM had an allosteric modulatory effect on HSCOMT. Absorption spectroscopy indicated adsorption of HSCOMT on the gold and silver NP surfaces and the formation of NPs-HSCOMT corona. Fluorescence spectroscopy showed that the interaction of HSCOMT with both gold and silver NPs was governed by a static quenching mechanism, implying the formation of a non-fluorescent fluorophore-NP complex at the ground state. Further fluorometric analyses indicated that both gold and silver NPs had contact with Trp143; that the interactions were spontaneous and were driven by electrostatic interactions. Fourier transform infrared spectroscopic studies showed the adsorption of HSCOMT of the NPs surfaces to cause relaxation of the enzyme’s B-sheet structures. Molecular docking studies indicated involvement of largely hydrophilic amino acids, with the interacting distances of less than 3.5A. These findings signify the potential of nanotechnology in the control of COMT catalytic activity for the management of the COMT-related disorders. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
The relationship between employability and labour market participation
- Uwuoruya, Nosayaba Frederick
- Authors: Uwuoruya, Nosayaba Frederick
- Date: 2018
- Subjects: Job hunting , Labor market -- Africa College graduates -- Employment -- Africa Career development
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/36101 , vital:33892
- Description: Unemployment is an urgent socio- economic issue facing not just Sub-Saharan Africa but also the global world at large (Yu, 2013). Globally, the International Labour Organisation (ILO) estimates that 201 million people were unemployed in 2014. Almost 74 million of those unemployed were young people (aged 15-24) in 2014. The ILO report further states that the number of people seeking employment was over 30 million more than in 2008, because several people lost their jobs due to the world economic recession. One of the results recorded from the ILO’s World Employment and Social Outlook 2016, show that youth unemployment is still three times that of the adult unemployment rate at 13.1% (ILO, 2016). Unemployed youths are estimated to be 73.3 million, a 3.3% decline from the global crisis in 2008 (ILO,2016:6). Crisp and Powell, (2016) indicate youth are vulnerable due to the economic fragility of the labour market and uneven recovery over the past years.
- Full Text:
- Date Issued: 2018
- Authors: Uwuoruya, Nosayaba Frederick
- Date: 2018
- Subjects: Job hunting , Labor market -- Africa College graduates -- Employment -- Africa Career development
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/36101 , vital:33892
- Description: Unemployment is an urgent socio- economic issue facing not just Sub-Saharan Africa but also the global world at large (Yu, 2013). Globally, the International Labour Organisation (ILO) estimates that 201 million people were unemployed in 2014. Almost 74 million of those unemployed were young people (aged 15-24) in 2014. The ILO report further states that the number of people seeking employment was over 30 million more than in 2008, because several people lost their jobs due to the world economic recession. One of the results recorded from the ILO’s World Employment and Social Outlook 2016, show that youth unemployment is still three times that of the adult unemployment rate at 13.1% (ILO, 2016). Unemployed youths are estimated to be 73.3 million, a 3.3% decline from the global crisis in 2008 (ILO,2016:6). Crisp and Powell, (2016) indicate youth are vulnerable due to the economic fragility of the labour market and uneven recovery over the past years.
- Full Text:
- Date Issued: 2018
The relationship between employability and labour market participation
- Uwuoruya, Nosayaba Frederick
- Authors: Uwuoruya, Nosayaba Frederick
- Date: 2018
- Subjects: Labor supply -- South Africa , Unemployment -- South Africa Work environment Work -- Social aspects
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/36111 , vital:33894
- Description: Unemployment is an urgent socio- economic issue facing not just Sub-Saharan Africa but also the global world at large (Yu, 2013). Globally, the International Labour Organisation (ILO) estimates that 201 million people were unemployed in 2014. Almost 74 million of those unemployed were young people (aged 15-24) in 2014. The ILO report further states that the number of people seeking employment was over 30 million more than in 2008, because several people lost their jobs due to the world economic recession. One of the results recorded from the ILO’s World Employment and Social Outlook 2016, show that youth unemployment is still three times that of the adult unemployment rate at 13.1% (ILO, 2016). Unemployed youths are estimated to be 73.3 million, a 3.3% decline from the global crisis in 2008 (ILO,2016:6). Crisp and Powell, (2016) indicate youth are vulnerable due to the economic fragility of the labour market and uneven recovery over the past years.The case of Sub-Saharan Africa is no different. South Africa is one of the countries grappling with high rates of unemployment, especially among youths (Burger & Fintel, 2009). According to a report by Statistics South Africa in 2015, 5.2 million of the labour force were unemployed, while 15.1 million South Africans are not economically active- resulting in an unemployment rate of 24.5% in the fourth quarter of 2015 (Statistics South Africa, 2015). Since- 2010, the first quarter of each year recorded increases in unemployment levels. The number of unemployed individuals had increase by more than half a million (626000, 521000) in the first quarter of 2015 and 2016 respectively (Statistics South Africa, 2016). The youth unemployment rate in South Africa decreased to 47.60% in the third quarter of 2016 from 53.70% in the second quarter of 2016.
- Full Text:
- Date Issued: 2018
- Authors: Uwuoruya, Nosayaba Frederick
- Date: 2018
- Subjects: Labor supply -- South Africa , Unemployment -- South Africa Work environment Work -- Social aspects
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/36111 , vital:33894
- Description: Unemployment is an urgent socio- economic issue facing not just Sub-Saharan Africa but also the global world at large (Yu, 2013). Globally, the International Labour Organisation (ILO) estimates that 201 million people were unemployed in 2014. Almost 74 million of those unemployed were young people (aged 15-24) in 2014. The ILO report further states that the number of people seeking employment was over 30 million more than in 2008, because several people lost their jobs due to the world economic recession. One of the results recorded from the ILO’s World Employment and Social Outlook 2016, show that youth unemployment is still three times that of the adult unemployment rate at 13.1% (ILO, 2016). Unemployed youths are estimated to be 73.3 million, a 3.3% decline from the global crisis in 2008 (ILO,2016:6). Crisp and Powell, (2016) indicate youth are vulnerable due to the economic fragility of the labour market and uneven recovery over the past years.The case of Sub-Saharan Africa is no different. South Africa is one of the countries grappling with high rates of unemployment, especially among youths (Burger & Fintel, 2009). According to a report by Statistics South Africa in 2015, 5.2 million of the labour force were unemployed, while 15.1 million South Africans are not economically active- resulting in an unemployment rate of 24.5% in the fourth quarter of 2015 (Statistics South Africa, 2015). Since- 2010, the first quarter of each year recorded increases in unemployment levels. The number of unemployed individuals had increase by more than half a million (626000, 521000) in the first quarter of 2015 and 2016 respectively (Statistics South Africa, 2016). The youth unemployment rate in South Africa decreased to 47.60% in the third quarter of 2016 from 53.70% in the second quarter of 2016.
- Full Text:
- Date Issued: 2018
A sociological investigation of popular music consumption among Nigerian youths living at Summerstrand and Humewood
- Uwuoruya, Osabuohien Clifford
- Authors: Uwuoruya, Osabuohien Clifford
- Date: 2018
- Subjects: Popular culture -- South Africa -- Port Elizabeth , Music and youth -- Africa Africa -- In popular culture Music and globalization -- Africa Socialism
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/36738 , vital:34049
- Description: The focus of this research study is on investigating how certain Nigerian youths expression of their agency at consuming popular music as a cultural product could be constrained by certain identified institutions and their gender in Nigeria. Using General Systems Theory (GST) as a theoretical framework, the country is presented as a social system with identified institutions which function within it that guides and constrains the agency of the individuals who live in Nigeria. A review of academic narratives relevant to the research is presented in this dissertation to provide a context for the study. A critical realist epistemology is adopted for this exploratory study, within the qualitative research tradition. A sample of twenty Nigerian youths living at Summerstrand and Humewood, is selected by the researcher using purposive sampling, and semi-structured interviews are conducted with these participants to gather their opinions and experiences regarding the research study. The narratives are analysed using thematic analysis, and the researcher presents an interpretation of these narratives under various topics in the dissertation. The findings from this study shows how some of these Nigerian youths believe their expression of agency at consuming popular music in Nigeria, to be constrained by certain institutions, and their gender in the country which they are part of, although this constraint could sometimes prove to be positive for some individuals. The recommendations from this research study are relevant to improving youth agency at consuming popular music in Nigeria, and advancing sociological inquiry into popular music and youth agency within an African context.
- Full Text:
- Date Issued: 2018
- Authors: Uwuoruya, Osabuohien Clifford
- Date: 2018
- Subjects: Popular culture -- South Africa -- Port Elizabeth , Music and youth -- Africa Africa -- In popular culture Music and globalization -- Africa Socialism
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/36738 , vital:34049
- Description: The focus of this research study is on investigating how certain Nigerian youths expression of their agency at consuming popular music as a cultural product could be constrained by certain identified institutions and their gender in Nigeria. Using General Systems Theory (GST) as a theoretical framework, the country is presented as a social system with identified institutions which function within it that guides and constrains the agency of the individuals who live in Nigeria. A review of academic narratives relevant to the research is presented in this dissertation to provide a context for the study. A critical realist epistemology is adopted for this exploratory study, within the qualitative research tradition. A sample of twenty Nigerian youths living at Summerstrand and Humewood, is selected by the researcher using purposive sampling, and semi-structured interviews are conducted with these participants to gather their opinions and experiences regarding the research study. The narratives are analysed using thematic analysis, and the researcher presents an interpretation of these narratives under various topics in the dissertation. The findings from this study shows how some of these Nigerian youths believe their expression of agency at consuming popular music in Nigeria, to be constrained by certain institutions, and their gender in the country which they are part of, although this constraint could sometimes prove to be positive for some individuals. The recommendations from this research study are relevant to improving youth agency at consuming popular music in Nigeria, and advancing sociological inquiry into popular music and youth agency within an African context.
- Full Text:
- Date Issued: 2018
Transformation through engagement: developing Grade 9 leadership opportunities through activity system using change laboratory intervention in a secondary school in Omusati region of Namibia
- Authors: Vaino, Loide Mwasheka
- Date: 2018
- Subjects: School management and organization Namibia Omusati , Student participation in administration Namibia Omusati , Educational leadership Namibia Omusati , Student government Namibia Omusati
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/61569 , vital:28038
- Description: The evolution of traditional educational leadership theory to contemporary leadership theory came as a response to an increased demand for better services in schools by creating platforms for learner leadership such as distributed leadership where learner leadership is located. This study of learner leadership is conducted in a secondary school in Omusati region of Namibia. Drawing on distributed leadership theory, the study sought to promote the distribution of leadership opportunities amongst all educational stakeholders, including learners, as provided for by policy and projected by educational leadership theory. The motivation of this study was the need to understand the problems associated with learner leadership as identified by past research. In addition, this study hoped to address the gap in the literature by exploring learner leadership development opportunities developing agency in learners through a Change Laboratory (CL) intervention. This study was as a transformative case study, how the grade 9s in the case study school were involved in leadership, the constraining factors that hindered the involvement of grade 9 learners in leadership, the enhancement of learner leadership at school, the positive contributions of CL workshops to participants and the leadership growth brought about by an intervention. This study generated data through observation, questionnaires, semi-structured interviews, focus group interviews, and Change Laboratory Workshops. The findings revealed that the grade 9 learners were marginally involved in leadership at the school. The most substantial challenge relates to traditional and outdated views of leadership on the part of teachers and educational managers. Additionally, the findings from the Change Laboratory workshops revealed that despite grade 9 learners being the youngest at school, they have the potential to be learner leaders. Hence in addition to several practical recommendations, the study recommends a change of mindset towards learner leadership so that opportunities are provided to contribute to the growth and development of learners. Finally, these research study findings will help my professional colleagues and policy makers in education to better understand the significance role of learner leadership involvements in schools.
- Full Text:
- Date Issued: 2018
- Authors: Vaino, Loide Mwasheka
- Date: 2018
- Subjects: School management and organization Namibia Omusati , Student participation in administration Namibia Omusati , Educational leadership Namibia Omusati , Student government Namibia Omusati
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/61569 , vital:28038
- Description: The evolution of traditional educational leadership theory to contemporary leadership theory came as a response to an increased demand for better services in schools by creating platforms for learner leadership such as distributed leadership where learner leadership is located. This study of learner leadership is conducted in a secondary school in Omusati region of Namibia. Drawing on distributed leadership theory, the study sought to promote the distribution of leadership opportunities amongst all educational stakeholders, including learners, as provided for by policy and projected by educational leadership theory. The motivation of this study was the need to understand the problems associated with learner leadership as identified by past research. In addition, this study hoped to address the gap in the literature by exploring learner leadership development opportunities developing agency in learners through a Change Laboratory (CL) intervention. This study was as a transformative case study, how the grade 9s in the case study school were involved in leadership, the constraining factors that hindered the involvement of grade 9 learners in leadership, the enhancement of learner leadership at school, the positive contributions of CL workshops to participants and the leadership growth brought about by an intervention. This study generated data through observation, questionnaires, semi-structured interviews, focus group interviews, and Change Laboratory Workshops. The findings revealed that the grade 9 learners were marginally involved in leadership at the school. The most substantial challenge relates to traditional and outdated views of leadership on the part of teachers and educational managers. Additionally, the findings from the Change Laboratory workshops revealed that despite grade 9 learners being the youngest at school, they have the potential to be learner leaders. Hence in addition to several practical recommendations, the study recommends a change of mindset towards learner leadership so that opportunities are provided to contribute to the growth and development of learners. Finally, these research study findings will help my professional colleagues and policy makers in education to better understand the significance role of learner leadership involvements in schools.
- Full Text:
- Date Issued: 2018
Vachellia erioloba (camel thorn) and microbial interactions
- Authors: Van Aswegen, Sunet
- Date: 2018
- Subjects: Vesicular-arbuscular mycorrhizas , Cadmium , Rhizobacteria , Plant growth-promoting rhizobacteria , Acacia erioloba
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/63716 , vital:28475
- Description: Vachellia erioloba (camel thorn) is one of South Africa’s economically important tree species and therefore requires further investigation to improve its health and growth. Beneficial soil microbes have positive effects on plants through various mechanisms such as nitrogen fixation, phosphate solubilisation, indole acetic acid and siderophore production and biofilm formation. These traits enhance plant growth and protect the host plant against parasitic organisms that are present in soil. The arbuscular mycorrhizal (AM) fungi are well known for their beneficial symbiotic effects on host plants. The objective of this study was to determine the role of AM fungi and associated beneficial rhizobacteria in improving the growth of V. erioloba seedlings. Soil and root samples were collected from a farm in the Northern Cape, South Africa. Fifty-seven bacterial cultures were isolated from the soil and tested for plant growth promoting characteristics. Fourteen isolates showing at least four beneficial traits were molecularly identified using the GenBank database. The AM fungal and bacterial populations in the soil samples were assessed using Illumina sequencing. Sequences were identified using the MaarJAM and GenBank databases, respectively. Three separate pot trials were conducted to determine; 1) the effects of cadmium (Cd) on seedling growth; 2) the individual effects of three selected bacterial isolates and AM fungi alone and combined on seedling growth, and 3) the combined effects of the selected bacteria on AM fungal inoculated and uninoculated seedlings. Of the fourteen isolates the Enterobacter genera was the dominant species identified, with Acinetobacter, Pantoea and Bacillus each having one isolate. All were described as plant growth promoting rhizobacteria. One isolate from each genus, excluding Pantoea, was used in the pot trials. Three genera were identified in the AM fungal population that was assessed, namely Ambispora, Paraglomus and Glomus with Ambispora being the dominant genus. The bacterial population assessed showed a high diversity of bacteria from the Actinobacteria phylum being the dominant group. The results of the heavy metal pot trial showed that the symbiotic relationship between the seedlings and AM fungi increased the seedlings’ health and growth during heavy metal stress. The combination of bacteria and AM fungi increased growth parameters in all the inoculated seedlings, but not when compared to uninoculated seedlings indicating a possible competition for nutrients. The results were influenced by the presence of a nematode, which was suspected to have been seed borne. Further investigations on these interactions are required. Inoculation of AM fungi and selected PGPR is recommended for V. erioloba seedling production. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
- Authors: Van Aswegen, Sunet
- Date: 2018
- Subjects: Vesicular-arbuscular mycorrhizas , Cadmium , Rhizobacteria , Plant growth-promoting rhizobacteria , Acacia erioloba
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/63716 , vital:28475
- Description: Vachellia erioloba (camel thorn) is one of South Africa’s economically important tree species and therefore requires further investigation to improve its health and growth. Beneficial soil microbes have positive effects on plants through various mechanisms such as nitrogen fixation, phosphate solubilisation, indole acetic acid and siderophore production and biofilm formation. These traits enhance plant growth and protect the host plant against parasitic organisms that are present in soil. The arbuscular mycorrhizal (AM) fungi are well known for their beneficial symbiotic effects on host plants. The objective of this study was to determine the role of AM fungi and associated beneficial rhizobacteria in improving the growth of V. erioloba seedlings. Soil and root samples were collected from a farm in the Northern Cape, South Africa. Fifty-seven bacterial cultures were isolated from the soil and tested for plant growth promoting characteristics. Fourteen isolates showing at least four beneficial traits were molecularly identified using the GenBank database. The AM fungal and bacterial populations in the soil samples were assessed using Illumina sequencing. Sequences were identified using the MaarJAM and GenBank databases, respectively. Three separate pot trials were conducted to determine; 1) the effects of cadmium (Cd) on seedling growth; 2) the individual effects of three selected bacterial isolates and AM fungi alone and combined on seedling growth, and 3) the combined effects of the selected bacteria on AM fungal inoculated and uninoculated seedlings. Of the fourteen isolates the Enterobacter genera was the dominant species identified, with Acinetobacter, Pantoea and Bacillus each having one isolate. All were described as plant growth promoting rhizobacteria. One isolate from each genus, excluding Pantoea, was used in the pot trials. Three genera were identified in the AM fungal population that was assessed, namely Ambispora, Paraglomus and Glomus with Ambispora being the dominant genus. The bacterial population assessed showed a high diversity of bacteria from the Actinobacteria phylum being the dominant group. The results of the heavy metal pot trial showed that the symbiotic relationship between the seedlings and AM fungi increased the seedlings’ health and growth during heavy metal stress. The combination of bacteria and AM fungi increased growth parameters in all the inoculated seedlings, but not when compared to uninoculated seedlings indicating a possible competition for nutrients. The results were influenced by the presence of a nematode, which was suspected to have been seed borne. Further investigations on these interactions are required. Inoculation of AM fungi and selected PGPR is recommended for V. erioloba seedling production. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
Graphite: origin, deposits and economics : an exploration study of the Orom Graphite project
- Van den Berg, Jacobus Petrus
- Authors: Van den Berg, Jacobus Petrus
- Date: 2018
- Subjects: Graphite , Ore deposits , Geophysics , Graphite mines and mining Economic aspects Africa, East , Trenches , Project management
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63786 , vital:28489
- Description: Developing exploration projects successfully requires that the Reasonable Prospects for Eventual Economic Extraction (RPEEE) be confirmed and based on the global market perception and trend. The exploration methods applied in the attempt to establish this RPEEE must be based on a key management framework that assures the results, and eventually the conclusion, are obtained with best practical and technical approaches whilst managing the risks and capitalizing on each result. The Orom Graphite project is located within the East African Orogenic belt, a suture zone between the Congo craton and the SLAMIN shield, formed during the formation of Gondwana during the late Proterozoic to early-Phanerozoic era. The closing of the Mozambique ocean, and the eventual collision between the craton and shield, occurred along the paleo-earths equator and migrated towards lower latitudes. This, along with the period’s biodiversity boom, provided the perfect deposition environment for carbonaceous sediments which were later metamorphosed to amphibolite and granulites grade metamorphism, resulting in the carbonization and the eventual graphitization of these carbonaceous sediments. The project is located within a poorly developed part of Uganda with the closest port situated some 1 500 km to the east in Kenya. The poorly developed infrastructure along with probable high logistical cost assigns a low competitivity index if compared to the economic costs of peer projects. However, the potential resources of the Orom Graphite project suggest that the Life of Mine (LOM) can rival the largest resource currently reported within the market. The current market conditions suggest that a possible oversupply of graphite concentrate will dominate the market within the next 4 to 10 years. This suggests that new graphite projects such as the Orom Graphite project are likely to develop into the production phase once the global supply and demand stabilize. This requires the Orom Graphite project to develop from its current scoping study level to a project development study level associated with a definitive feasibility study. To date, the project developed through mapping, reconnaissance drilling, geophysical survey and trenching programs increasing the Net Present Value (NPV) considerably based upon a Cost-Based Valuation approach using Prospectivity Enhancement Multiplier (PEM). The metallurgical studies could however not produce a graphite concentrate product within industrial grade standards. The risk associated with developing the project further into the Mineral Resource Estimation (MRE) phase was quantified and risk was evaluated by implementing a point decision tree and calculating the Expected Monetary Value (EMV). Due to the unfavourable metallurgical results obtained to date, the risk associated with undertaking an additional metallurgical test is considerable with a slight chance of producing a negative project value estimated at 65%. JP van den Berg Rhodes University Overall, the Orom Graphite project contains favourable geological formations with a potential large resource. Market trends indicate that a considerable resource is currently being developed and can supply the global market for the next 4 to 10 years. The project’s location within a landlocked country decreases its economic competitiveness with peer project and the unfavourable, but not conclusive, metallurgical results obtained during the scoping phase do not instil confidence that the project will develop into a productive mine soon. Managing the project development with future graphite demand in mind is the key to determining whether the project still has future value.
- Full Text:
- Date Issued: 2018
- Authors: Van den Berg, Jacobus Petrus
- Date: 2018
- Subjects: Graphite , Ore deposits , Geophysics , Graphite mines and mining Economic aspects Africa, East , Trenches , Project management
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63786 , vital:28489
- Description: Developing exploration projects successfully requires that the Reasonable Prospects for Eventual Economic Extraction (RPEEE) be confirmed and based on the global market perception and trend. The exploration methods applied in the attempt to establish this RPEEE must be based on a key management framework that assures the results, and eventually the conclusion, are obtained with best practical and technical approaches whilst managing the risks and capitalizing on each result. The Orom Graphite project is located within the East African Orogenic belt, a suture zone between the Congo craton and the SLAMIN shield, formed during the formation of Gondwana during the late Proterozoic to early-Phanerozoic era. The closing of the Mozambique ocean, and the eventual collision between the craton and shield, occurred along the paleo-earths equator and migrated towards lower latitudes. This, along with the period’s biodiversity boom, provided the perfect deposition environment for carbonaceous sediments which were later metamorphosed to amphibolite and granulites grade metamorphism, resulting in the carbonization and the eventual graphitization of these carbonaceous sediments. The project is located within a poorly developed part of Uganda with the closest port situated some 1 500 km to the east in Kenya. The poorly developed infrastructure along with probable high logistical cost assigns a low competitivity index if compared to the economic costs of peer projects. However, the potential resources of the Orom Graphite project suggest that the Life of Mine (LOM) can rival the largest resource currently reported within the market. The current market conditions suggest that a possible oversupply of graphite concentrate will dominate the market within the next 4 to 10 years. This suggests that new graphite projects such as the Orom Graphite project are likely to develop into the production phase once the global supply and demand stabilize. This requires the Orom Graphite project to develop from its current scoping study level to a project development study level associated with a definitive feasibility study. To date, the project developed through mapping, reconnaissance drilling, geophysical survey and trenching programs increasing the Net Present Value (NPV) considerably based upon a Cost-Based Valuation approach using Prospectivity Enhancement Multiplier (PEM). The metallurgical studies could however not produce a graphite concentrate product within industrial grade standards. The risk associated with developing the project further into the Mineral Resource Estimation (MRE) phase was quantified and risk was evaluated by implementing a point decision tree and calculating the Expected Monetary Value (EMV). Due to the unfavourable metallurgical results obtained to date, the risk associated with undertaking an additional metallurgical test is considerable with a slight chance of producing a negative project value estimated at 65%. JP van den Berg Rhodes University Overall, the Orom Graphite project contains favourable geological formations with a potential large resource. Market trends indicate that a considerable resource is currently being developed and can supply the global market for the next 4 to 10 years. The project’s location within a landlocked country decreases its economic competitiveness with peer project and the unfavourable, but not conclusive, metallurgical results obtained during the scoping phase do not instil confidence that the project will develop into a productive mine soon. Managing the project development with future graphite demand in mind is the key to determining whether the project still has future value.
- Full Text:
- Date Issued: 2018
Vulnerabilities of Coastal Tourism destinations to climate change related incidents: a case study selected holiday resorts along the wildcoast, Eastern Cape, South Africa
- Authors: Van Der Byl, Athina
- Date: 2018
- Subjects: Coastal Tourism destinations -- Wildcoast Wildcoast -- Holiday resorts -- South Africa -- Eastern Cape Climate change -- Tourism
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1337 , vital:32259
- Description: Climate change is a global phenomenon with major impacts on coastlines, leaving coastal areas vulnerable to conditions such as sea level rise, flooding as well as storm surges, which results in increased damage to or loss of coastal property and infrastructure. The study examined the vulnerability of the Eastern Cape’s Wild Coast resorts to climate change related impacts. Both qualitative and quantitative methods aided by questionnaires and GIS mapping were used to identify vulnerable settlements as well as their impacts. A meta analysis of the identified vulnerabilities was studied and strategies employed to reduce the impacts was also done. Results indicated that almost 80% of the Wild Coast resorts occur within the low-lying areas of the coast and these areas were mostly affected by impacts such as sea level rise, heavy rainfall and floods accompanied by storm surges. Another alarming challenge faced by the King Sabata Dalindyebo Municipality was controlling unplanned developments within these low-lying zones of the coast. Therefore, it is recommended that relevant departments provide awareness through various platforms such as workshops, programmes and campaigns to ensure that people understand the risk of climate change on low-lying areas as well as mainstreaming climate change in long term development planning. This study highlights a need for monitoring of coastal environments vulnerable to the impact of climate change along a South African coastline.
- Full Text:
- Date Issued: 2018
- Authors: Van Der Byl, Athina
- Date: 2018
- Subjects: Coastal Tourism destinations -- Wildcoast Wildcoast -- Holiday resorts -- South Africa -- Eastern Cape Climate change -- Tourism
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1337 , vital:32259
- Description: Climate change is a global phenomenon with major impacts on coastlines, leaving coastal areas vulnerable to conditions such as sea level rise, flooding as well as storm surges, which results in increased damage to or loss of coastal property and infrastructure. The study examined the vulnerability of the Eastern Cape’s Wild Coast resorts to climate change related impacts. Both qualitative and quantitative methods aided by questionnaires and GIS mapping were used to identify vulnerable settlements as well as their impacts. A meta analysis of the identified vulnerabilities was studied and strategies employed to reduce the impacts was also done. Results indicated that almost 80% of the Wild Coast resorts occur within the low-lying areas of the coast and these areas were mostly affected by impacts such as sea level rise, heavy rainfall and floods accompanied by storm surges. Another alarming challenge faced by the King Sabata Dalindyebo Municipality was controlling unplanned developments within these low-lying zones of the coast. Therefore, it is recommended that relevant departments provide awareness through various platforms such as workshops, programmes and campaigns to ensure that people understand the risk of climate change on low-lying areas as well as mainstreaming climate change in long term development planning. This study highlights a need for monitoring of coastal environments vulnerable to the impact of climate change along a South African coastline.
- Full Text:
- Date Issued: 2018
The secret life of public sculpture: an explorative case study on what the “voting line” does in Central, Port Elizabeth
- Authors: Van der Merwe, Joy
- Date: 2018
- Subjects: Public art , Public art -- South Africa -- Port Elizabeth Art and society Sculpture -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/36750 , vital:34050
- Description: To date, there has been little contemporary academic study on public art in Port Elizabeth. There have however been major art-led urban regeneration projects in the last ten years. This raises the question of what these artworks have been ‘doing’ in their relatively new homes. This study explores the function of the Voting Line, which was commissioned by the Mandela Bay Development Agency and made by Anthony Harris and Konrad Geel. The Voting Line is discussed as a key example of public artwork produced for Route 67. Using a case study design, four different approaches have been applied in this exploratory study. These include: document analysis, observation, interviews and a South African data making process called the Mmogo Method. The findings of this study suggest that the Voting Line is broadly considered as contributing towards nation building; cultural heritage tourism, and developing city identity.
- Full Text:
- Date Issued: 2018
- Authors: Van der Merwe, Joy
- Date: 2018
- Subjects: Public art , Public art -- South Africa -- Port Elizabeth Art and society Sculpture -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/36750 , vital:34050
- Description: To date, there has been little contemporary academic study on public art in Port Elizabeth. There have however been major art-led urban regeneration projects in the last ten years. This raises the question of what these artworks have been ‘doing’ in their relatively new homes. This study explores the function of the Voting Line, which was commissioned by the Mandela Bay Development Agency and made by Anthony Harris and Konrad Geel. The Voting Line is discussed as a key example of public artwork produced for Route 67. Using a case study design, four different approaches have been applied in this exploratory study. These include: document analysis, observation, interviews and a South African data making process called the Mmogo Method. The findings of this study suggest that the Voting Line is broadly considered as contributing towards nation building; cultural heritage tourism, and developing city identity.
- Full Text:
- Date Issued: 2018
Yeast-baculovirus synergism: investigating mixed infections for improved management of the false codling moth, Thaumatotibia leucotreta
- Authors: Van der Merwe, Marcel
- Date: 2018
- Subjects: Cryptophlebia leucotreta , Baculoviruses , Yeast , Citrus Diseases and pests , Biological pest control agents , Pests Integrated control
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/62963 , vital:28347
- Description: Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) or otherwise commonly known as the false codling moth is an indigenous pest of the citrus industry in southern Africa. The pest is highly significant as it impacts negatively on the export of fresh citrus fruits from South Africa to international markets. To control T. leucotreta in South Africa, an integrated pest management (IPM) programme has been implemented. One component of this programme is the baculovirus Cryptophlebia leucotreta granulovirus (CrleGV-SA) which has been formulated into the products Cryptogran™ and Cryptex®. It has previously been reported that there is a mutualistic association between Cydia pomonella (L.) (Lepidoptera: Tortricidae) also known as codling moth, and epiphytic yeasts. Cydia pomonella larval feeding galleries were colonised by yeasts and this, in turn, reduced larval mortality and enhanced larval development. It has been demonstrated in laboratory assays and field trials that combining yeast and brown cane sugar with Cydia pomonella granulovirus (CpGV) significantly increased larval mortality and lowered the proportion of injured apple fruit. This suggests that yeasts can enhance the effectiveness of an insect virus in managing pest larvae. In this study, we proposed to determine which species of yeast occur naturally in the digestive tract, frass and on the epidermis of T. leucotreta larvae and to examine whether any of these yeasts, when combined with the CrleGV-SA, have a synergistic effect in increasing mortality of T. leucotreta larvae. Firstly, Navel oranges infested with T. leucotreta larvae were collected from orchards in Sundays River Valley in Eastern Cape of South Africa. Larvae were extracted and analysed for the presence of yeast on their surface, or in their gut and frass. Four yeasts were isolated from T. leucotreta larvae and identified down to species level via PCR amplification and sequencing of internal transcribed spacer (ITS) region and D1/D2 domain of the large subunit (LSU) of rDNA region. These yeasts were isolated from the frass, epidermis and digestive tract of T. leucotreta larvae. The yeast isolates were identified as Meyerozyma caribbica, Pichia kluyveri, Pichia kudriavzevii and Hanseniaspora opuntiae. A yeast preference assay was conducted on female T. leucotreta moths to examine whether any of the isolated yeast species affected their oviposition preference. Navel oranges were inoculated with the isolated yeast species at a concentration of 6 × 108 cells.ml-1. The assay also included a Brewer’s yeast and distilled water control. Pichia kudriavzevii was shown to be the preferred yeast species for oviposition, as significantly more eggs were deposited on Navel oranges inoculated with this yeast compared to the other treatments. Lastly, a detached fruit bioassay was performed to evaluate the efficacy of mixing P. kudriavzevii with CrleGV-SA to enhance T. leucotreta larvae mortality. Pichia kudriavzevii was selected as it was demonstrated as having an effect on the oviposition preference of female T. leucotreta moths. The concentration at which P. kudriavzevii was applied remained the same as in the preference assay while CrleGV-SA was applied at lethal concentration required to kill 50 % of the population (9.31 × 107 OBs.ml-1). Although an increase in larval mortality was observed between CrleGV-SA being applied alone and the yeast/virus mixture, this result was determined not to be statistically significant. The experiments performed in this study provide a platform for further research into the application of a yeast-virus combination as a novel control option for T. leucotreta in the field. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
- Authors: Van der Merwe, Marcel
- Date: 2018
- Subjects: Cryptophlebia leucotreta , Baculoviruses , Yeast , Citrus Diseases and pests , Biological pest control agents , Pests Integrated control
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/62963 , vital:28347
- Description: Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) or otherwise commonly known as the false codling moth is an indigenous pest of the citrus industry in southern Africa. The pest is highly significant as it impacts negatively on the export of fresh citrus fruits from South Africa to international markets. To control T. leucotreta in South Africa, an integrated pest management (IPM) programme has been implemented. One component of this programme is the baculovirus Cryptophlebia leucotreta granulovirus (CrleGV-SA) which has been formulated into the products Cryptogran™ and Cryptex®. It has previously been reported that there is a mutualistic association between Cydia pomonella (L.) (Lepidoptera: Tortricidae) also known as codling moth, and epiphytic yeasts. Cydia pomonella larval feeding galleries were colonised by yeasts and this, in turn, reduced larval mortality and enhanced larval development. It has been demonstrated in laboratory assays and field trials that combining yeast and brown cane sugar with Cydia pomonella granulovirus (CpGV) significantly increased larval mortality and lowered the proportion of injured apple fruit. This suggests that yeasts can enhance the effectiveness of an insect virus in managing pest larvae. In this study, we proposed to determine which species of yeast occur naturally in the digestive tract, frass and on the epidermis of T. leucotreta larvae and to examine whether any of these yeasts, when combined with the CrleGV-SA, have a synergistic effect in increasing mortality of T. leucotreta larvae. Firstly, Navel oranges infested with T. leucotreta larvae were collected from orchards in Sundays River Valley in Eastern Cape of South Africa. Larvae were extracted and analysed for the presence of yeast on their surface, or in their gut and frass. Four yeasts were isolated from T. leucotreta larvae and identified down to species level via PCR amplification and sequencing of internal transcribed spacer (ITS) region and D1/D2 domain of the large subunit (LSU) of rDNA region. These yeasts were isolated from the frass, epidermis and digestive tract of T. leucotreta larvae. The yeast isolates were identified as Meyerozyma caribbica, Pichia kluyveri, Pichia kudriavzevii and Hanseniaspora opuntiae. A yeast preference assay was conducted on female T. leucotreta moths to examine whether any of the isolated yeast species affected their oviposition preference. Navel oranges were inoculated with the isolated yeast species at a concentration of 6 × 108 cells.ml-1. The assay also included a Brewer’s yeast and distilled water control. Pichia kudriavzevii was shown to be the preferred yeast species for oviposition, as significantly more eggs were deposited on Navel oranges inoculated with this yeast compared to the other treatments. Lastly, a detached fruit bioassay was performed to evaluate the efficacy of mixing P. kudriavzevii with CrleGV-SA to enhance T. leucotreta larvae mortality. Pichia kudriavzevii was selected as it was demonstrated as having an effect on the oviposition preference of female T. leucotreta moths. The concentration at which P. kudriavzevii was applied remained the same as in the preference assay while CrleGV-SA was applied at lethal concentration required to kill 50 % of the population (9.31 × 107 OBs.ml-1). Although an increase in larval mortality was observed between CrleGV-SA being applied alone and the yeast/virus mixture, this result was determined not to be statistically significant. The experiments performed in this study provide a platform for further research into the application of a yeast-virus combination as a novel control option for T. leucotreta in the field. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
Factors affecting effective ecological restoration of Portulacaria afra (spekboom)-rich subtropical thicket and aboveground carbon endpoint projections
- Van der Vyver, Marius Lodewyk
- Authors: Van der Vyver, Marius Lodewyk
- Date: 2018
- Subjects: Restoration ecology , Plant conservation -- South Africa Portulacaria afra -- South Africa Shrubs -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23250 , vital:30468
- Description: Among the requirements to achieve effective ecological restoration on a landscape scale are a scientific underpinning, strong adaptive management and the setting of realistic endpoints. Efficacy and success depend on a complex interplay of factors of both local and regional influence. Biome-wide restoration experiments are rare, but valuable for evaluating efficacy of different protocols according to local context. The Thicket-wide Plot (TWP) experiment was initiated by the Subtropical Thicket Restoration Programme to inform landscape-scale ecological restoration of degraded Portulacaria afra (spekboom)-rich Subtropical Thicket. Thirteen different planting treatments of Portulacaria afra (spekboom) truncheons were planted as replicated rows on 0.25 ha fenced plots across the distribution range of spekboom-rich thicket habitat to better establish effective restoration protocols. I used a rule-based learning ensemble algorithm to evaluate 60 different predictors that potentially impact effective restoration, covering a range of climatic, topographic, edaphic, ecological and management related factors observed at 227 of these TWP plots. Percentage survivorship and aboveground biomass carbon sequestration rate (ABCsr) were taken as proxies for efficacy derived from subsequent monitoring data gathered 2 - 5 years after establishment, and a new allometric model I developed for spekboom. I found herbivory and identification of target habitat the two most important predictors of restoration efficacy, both well within the control of the practitioner. Highest survivorship and ABCsr were associated with larger truncheons planted deeper. The only exception to this pattern was found amongst the two smallest size truncheon categories. The application of external rooting hormone or watering treatment to truncheons were found of no significance to effective restoration, while all the pruned treatments were, in association with lesser planting depth, found to be significantly less effective than untreated truncheons planted deeper. When the restoration protocol is optimal (largest truncheons planted deepest in target habitats and protected from herbivory), my results show higher annual carbon sequestration rates (1.46 - 3.7 t C ha−1 yr−1 in aboveground biomass) than most older restoration sites that have been identified as benchmarks of success. In order to project ABC endpoints, I generated 40 new species-specific allometric models and used them to estimate biomass and carbon of intact and degraded stands on five sites spread out across the region. The highest AGC for stands in both intact and degraded states were estimated at 42.96 t Cha−1 and 12.98 t C ha−1 respectively, and the lowest at 26.32 t C ha−1 and 2.52 t C ha−1. Large canopy dominant (LCD) species contributed the largest AGC portion at three intact stands. The second largest portion was recorded for spekboom. The difference between paired stands on each sample site ranged between 23.8 and 32.8 t C ha−1, which provide a realistic target for biome-wide restoration that, when adopted together with current protocols, may be reached within three decades of inception.
- Full Text:
- Date Issued: 2018
- Authors: Van der Vyver, Marius Lodewyk
- Date: 2018
- Subjects: Restoration ecology , Plant conservation -- South Africa Portulacaria afra -- South Africa Shrubs -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23250 , vital:30468
- Description: Among the requirements to achieve effective ecological restoration on a landscape scale are a scientific underpinning, strong adaptive management and the setting of realistic endpoints. Efficacy and success depend on a complex interplay of factors of both local and regional influence. Biome-wide restoration experiments are rare, but valuable for evaluating efficacy of different protocols according to local context. The Thicket-wide Plot (TWP) experiment was initiated by the Subtropical Thicket Restoration Programme to inform landscape-scale ecological restoration of degraded Portulacaria afra (spekboom)-rich Subtropical Thicket. Thirteen different planting treatments of Portulacaria afra (spekboom) truncheons were planted as replicated rows on 0.25 ha fenced plots across the distribution range of spekboom-rich thicket habitat to better establish effective restoration protocols. I used a rule-based learning ensemble algorithm to evaluate 60 different predictors that potentially impact effective restoration, covering a range of climatic, topographic, edaphic, ecological and management related factors observed at 227 of these TWP plots. Percentage survivorship and aboveground biomass carbon sequestration rate (ABCsr) were taken as proxies for efficacy derived from subsequent monitoring data gathered 2 - 5 years after establishment, and a new allometric model I developed for spekboom. I found herbivory and identification of target habitat the two most important predictors of restoration efficacy, both well within the control of the practitioner. Highest survivorship and ABCsr were associated with larger truncheons planted deeper. The only exception to this pattern was found amongst the two smallest size truncheon categories. The application of external rooting hormone or watering treatment to truncheons were found of no significance to effective restoration, while all the pruned treatments were, in association with lesser planting depth, found to be significantly less effective than untreated truncheons planted deeper. When the restoration protocol is optimal (largest truncheons planted deepest in target habitats and protected from herbivory), my results show higher annual carbon sequestration rates (1.46 - 3.7 t C ha−1 yr−1 in aboveground biomass) than most older restoration sites that have been identified as benchmarks of success. In order to project ABC endpoints, I generated 40 new species-specific allometric models and used them to estimate biomass and carbon of intact and degraded stands on five sites spread out across the region. The highest AGC for stands in both intact and degraded states were estimated at 42.96 t Cha−1 and 12.98 t C ha−1 respectively, and the lowest at 26.32 t C ha−1 and 2.52 t C ha−1. Large canopy dominant (LCD) species contributed the largest AGC portion at three intact stands. The second largest portion was recorded for spekboom. The difference between paired stands on each sample site ranged between 23.8 and 32.8 t C ha−1, which provide a realistic target for biome-wide restoration that, when adopted together with current protocols, may be reached within three decades of inception.
- Full Text:
- Date Issued: 2018
The visual communication of environmental awareness issues in Jeff Orlowski's,Chasing Ice (2012) and Yann Arthurs -Bertrand's home (2009)
- Authors: Van der Walt, Nadia Elize
- Date: 2018
- Subjects: Visual communication , Environmentalism in motion pictures Documentary films -- History and critism
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/45547 , vital:38665
- Description: This study presents an investigation into the visual communication complexities within the genre of documentary film, specifically aimed at the development of a set of criteria of cinematic techniques for the visual communication of environmental awareness issues. This process utilises a theoretical approach to understanding the development and communicative possibilities of documentary film, as well as an analytical interpretation structured on semiotic film theory. The theoretical investigation reveals Bill Nichols’ (2010) documentary modes as an established analytical model. This study engages with four of Nichols’ six modes – namely, the poetic, the expository, the observational and the participatory modes, as the criteria for the extraction of scenes and/or images from Chasing Ice (2012) by Jeff Orlowski and Home (2009) by Yann Arthurs-Bertrand. Once Nichols’ modes have been identified, a semiotic reading is conducted. Gillian Rose’s (2016) visual analysis framework underpinned by Pieter J. Fourie’s (1988) sociological approach to film analysis, is utilised to read the selected film texts. A comparative analysis of Chasing Ice (2012) and Home (2009) reveals that the inclusion of different and multiple modes constructs the visualisation of environmental awareness issues in the documentary film genre. The cinematic techniques specific to the documentary modes represented in Chasing Ice (2012) and Home (2009) are appropriated in the construction of three film shorts within the researcher’s documentary film study, Karoo (2017). This combination of theory and practice yields the researcher a considered and informed approach to constructing documentary imagery aimed at visualising the current environment of the Karoo Basin prior to the possibility of shale gas exploration and/or exploitation.
- Full Text:
- Date Issued: 2018
- Authors: Van der Walt, Nadia Elize
- Date: 2018
- Subjects: Visual communication , Environmentalism in motion pictures Documentary films -- History and critism
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/45547 , vital:38665
- Description: This study presents an investigation into the visual communication complexities within the genre of documentary film, specifically aimed at the development of a set of criteria of cinematic techniques for the visual communication of environmental awareness issues. This process utilises a theoretical approach to understanding the development and communicative possibilities of documentary film, as well as an analytical interpretation structured on semiotic film theory. The theoretical investigation reveals Bill Nichols’ (2010) documentary modes as an established analytical model. This study engages with four of Nichols’ six modes – namely, the poetic, the expository, the observational and the participatory modes, as the criteria for the extraction of scenes and/or images from Chasing Ice (2012) by Jeff Orlowski and Home (2009) by Yann Arthurs-Bertrand. Once Nichols’ modes have been identified, a semiotic reading is conducted. Gillian Rose’s (2016) visual analysis framework underpinned by Pieter J. Fourie’s (1988) sociological approach to film analysis, is utilised to read the selected film texts. A comparative analysis of Chasing Ice (2012) and Home (2009) reveals that the inclusion of different and multiple modes constructs the visualisation of environmental awareness issues in the documentary film genre. The cinematic techniques specific to the documentary modes represented in Chasing Ice (2012) and Home (2009) are appropriated in the construction of three film shorts within the researcher’s documentary film study, Karoo (2017). This combination of theory and practice yields the researcher a considered and informed approach to constructing documentary imagery aimed at visualising the current environment of the Karoo Basin prior to the possibility of shale gas exploration and/or exploitation.
- Full Text:
- Date Issued: 2018
Exploring and implementing Open Linked Data record creation on the Rhodes Digital Commons
- Authors: Van der Walt, Wynand
- Date: 2018-07-04
- Subjects: Linked data , Academic libraries -- Automation -- South Africa
- Language: English
- Type: text , conference materials
- Identifier: http://hdl.handle.net/10962/65066 , vital:28661
- Description: Presentation at the NeDICC Meeting, held on the 4th of July 2018 at the CSIR, Pretoria. The purpose of the presentation was to inform the NeDICC community members of preliminary investigations into Linked Data and the potential impact on the Rhodes Digital Commons (Rhodes' Institutional Repository).
- Full Text:
- Date Issued: 2018-07-04
- Authors: Van der Walt, Wynand
- Date: 2018-07-04
- Subjects: Linked data , Academic libraries -- Automation -- South Africa
- Language: English
- Type: text , conference materials
- Identifier: http://hdl.handle.net/10962/65066 , vital:28661
- Description: Presentation at the NeDICC Meeting, held on the 4th of July 2018 at the CSIR, Pretoria. The purpose of the presentation was to inform the NeDICC community members of preliminary investigations into Linked Data and the potential impact on the Rhodes Digital Commons (Rhodes' Institutional Repository).
- Full Text:
- Date Issued: 2018-07-04
The relationship between psychological capital, life satisfaction and employee retention
- Authors: Van Greuning, Hester
- Date: 2018
- Subjects: Employee Retention Work -- Psychological aspects Job satisfaction
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/23847 , vital:30625
- Description: In order to achieve sustainable employer environments, South African organisations are faced with the challenge of retaining key and critical employee cohorts. Externally focused methods and models of turnover and retention fail to comprehensively explain the lack of highly skilled employees and the inability of organisations to successfully retain these employee groupings. This non experimental correlational study investigated the relationship and interaction between the positive psychological constructs of psychological capital and life satisfaction and employee turnover intention of a South African sample of specialist and management level employees (n=150). The survey method was utilised to gather data for the study with a biographical questionnaire, the Psychological Capital Questionnaire-12 (PCQ-12), the Satisfaction with Life Scale (SWLS) and the Turnover Intention Scale-6 (TIS-6). Data analysis was conducted in the form of descriptive and inferential statistics including frequency tables, Cronbach’s Alpha testing, exploratory factory analysis, correlation analysis, t-tests, analysis of variance and structural equation modelling. The results indicated that significant relationships exist between psychological capital, life satisfaction and employee turnover intention. A substantial negative relationship was found between psychological capital and employee turnover intention, as well as a small, yet definite negative relationship between life satisfaction and turnover intention. Even though the results indicated a substantial positive relationship between psychological capital and life satisfaction, the relationship with employee turnover intention was insignificant when the two variables were correlated. This study adds to the current body of knowledge regarding employee retention in South Africa, and allows for a number of future research avenues to be explored.
- Full Text:
- Date Issued: 2018
- Authors: Van Greuning, Hester
- Date: 2018
- Subjects: Employee Retention Work -- Psychological aspects Job satisfaction
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/23847 , vital:30625
- Description: In order to achieve sustainable employer environments, South African organisations are faced with the challenge of retaining key and critical employee cohorts. Externally focused methods and models of turnover and retention fail to comprehensively explain the lack of highly skilled employees and the inability of organisations to successfully retain these employee groupings. This non experimental correlational study investigated the relationship and interaction between the positive psychological constructs of psychological capital and life satisfaction and employee turnover intention of a South African sample of specialist and management level employees (n=150). The survey method was utilised to gather data for the study with a biographical questionnaire, the Psychological Capital Questionnaire-12 (PCQ-12), the Satisfaction with Life Scale (SWLS) and the Turnover Intention Scale-6 (TIS-6). Data analysis was conducted in the form of descriptive and inferential statistics including frequency tables, Cronbach’s Alpha testing, exploratory factory analysis, correlation analysis, t-tests, analysis of variance and structural equation modelling. The results indicated that significant relationships exist between psychological capital, life satisfaction and employee turnover intention. A substantial negative relationship was found between psychological capital and employee turnover intention, as well as a small, yet definite negative relationship between life satisfaction and turnover intention. Even though the results indicated a substantial positive relationship between psychological capital and life satisfaction, the relationship with employee turnover intention was insignificant when the two variables were correlated. This study adds to the current body of knowledge regarding employee retention in South Africa, and allows for a number of future research avenues to be explored.
- Full Text:
- Date Issued: 2018
The relationship between psychological capital,life satisfaction and employee retention
- Authors: Van Greuning, Hester
- Date: 2018
- Subjects: Employee Retention , Work -- Psychological aspects , Job satisfaction
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/45712 , vital:38957
- Description: South African organisations are faced with the challenge of retaining key and critical employee cohorts. externally focused methods and models of turnover and retention fail to comprehensively explain the lack of highly skilled employees and the inability of organisations to successfully retain these employee groupings. This nonexperimental correlational study investigated the relationship and interaction between the positive psychological constructs of psychological capital and life satisfaction and employee turnover intention of a South African sample of specialist and management level employees (n=150). The survey method was utilised to gather data for the study with a biographical questionnaire, the Psychological Capital Questionnaire-12 (PCQ-12), the Satisfaction with Life Scale (SWLS) and the Turnover Intention Scale-6 (TIS-6). Data analysis was conducted in the form of descriptive and inferential statistics including frequency tables, Cronbach’s Alpha testing, exploratory factory analysis, correlation analysis, t-tests, analysis of variance and structural equation modelling. The results indicated that significant relationships exist between psychological capital, life satisfaction and employee turnover intention. A substantial negative relationship was found between psychological capital and employee turnover intention, as well as a small, yet definite negative relationship between life satisfaction and turnover intention. Even though the results indicated a substantial positive relationship between psychological capital and life satisfaction, the relationship with employee turnover intention was insignificant when the two variables were correlated. This study adds to the current body of knowledge regarding employee retention in South Africa, and allows for a number of future research avenues to be explored.
- Full Text:
- Date Issued: 2018
- Authors: Van Greuning, Hester
- Date: 2018
- Subjects: Employee Retention , Work -- Psychological aspects , Job satisfaction
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/45712 , vital:38957
- Description: South African organisations are faced with the challenge of retaining key and critical employee cohorts. externally focused methods and models of turnover and retention fail to comprehensively explain the lack of highly skilled employees and the inability of organisations to successfully retain these employee groupings. This nonexperimental correlational study investigated the relationship and interaction between the positive psychological constructs of psychological capital and life satisfaction and employee turnover intention of a South African sample of specialist and management level employees (n=150). The survey method was utilised to gather data for the study with a biographical questionnaire, the Psychological Capital Questionnaire-12 (PCQ-12), the Satisfaction with Life Scale (SWLS) and the Turnover Intention Scale-6 (TIS-6). Data analysis was conducted in the form of descriptive and inferential statistics including frequency tables, Cronbach’s Alpha testing, exploratory factory analysis, correlation analysis, t-tests, analysis of variance and structural equation modelling. The results indicated that significant relationships exist between psychological capital, life satisfaction and employee turnover intention. A substantial negative relationship was found between psychological capital and employee turnover intention, as well as a small, yet definite negative relationship between life satisfaction and turnover intention. Even though the results indicated a substantial positive relationship between psychological capital and life satisfaction, the relationship with employee turnover intention was insignificant when the two variables were correlated. This study adds to the current body of knowledge regarding employee retention in South Africa, and allows for a number of future research avenues to be explored.
- Full Text:
- Date Issued: 2018
Major security incidents since 2014: an African perspective
- Van Heerden, Renier, Von Solms, Sune, Vorster, Johannes
- Authors: Van Heerden, Renier , Von Solms, Sune , Vorster, Johannes
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68291 , vital:29234 , https://ieeexplore.ieee.org/abstract/document/8417326/
- Description: Publisher version , The integration of technology in the modern society provides many benefits, but with increased connectivity comes increased risk where governments, businesses and individuals are vulnerable to a variety of cyber-attacks. Many of the large information security attacks of the last decade can be seen as an attack on 'foreign” systems or individuals when viewed from an African perspective, with no direct impact on an individual in Africa. However, information security experts in Africa states that although some of these attacks might not have had a direct impact of the African individual, but never the less should not be ignored as it does indirectly influence the African individual. The experts state that even if the individuals or businesses are not directly influenced by an attack, it should not be ignored as similar attacks might influence them in the future. They emphasise that these attacks should improve their cybersecurity awareness and behaviour, in order to prevent similar attacks from impacting them.
- Full Text: false
- Date Issued: 2018
- Authors: Van Heerden, Renier , Von Solms, Sune , Vorster, Johannes
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68291 , vital:29234 , https://ieeexplore.ieee.org/abstract/document/8417326/
- Description: Publisher version , The integration of technology in the modern society provides many benefits, but with increased connectivity comes increased risk where governments, businesses and individuals are vulnerable to a variety of cyber-attacks. Many of the large information security attacks of the last decade can be seen as an attack on 'foreign” systems or individuals when viewed from an African perspective, with no direct impact on an individual in Africa. However, information security experts in Africa states that although some of these attacks might not have had a direct impact of the African individual, but never the less should not be ignored as it does indirectly influence the African individual. The experts state that even if the individuals or businesses are not directly influenced by an attack, it should not be ignored as similar attacks might influence them in the future. They emphasise that these attacks should improve their cybersecurity awareness and behaviour, in order to prevent similar attacks from impacting them.
- Full Text: false
- Date Issued: 2018