In vitro evaluation of antimicrobial and antioxidant activities of olea europaea subsp. africana and euryops brevipapposus used by Cala community folkloric medicine for the management of infections associated with chronic non-communicable diseases
- Authors: Adegborioye, Abiodun
- Date: 2016
- Subjects: Antioxidants , Medicinal plants , Traditional medicine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4869 , vital:28624
- Description: Chronic non-communicable diseses are a global public health challenge that continuously threatens the development and health of humans. Risk factors such as unbalanced diet-the high consumption of processed food or food from animal origin are responsible for NCDs. NCDs result in weakened immune system, making the host susceptible to opportunistic infections. Thus, the NCDs burden is most times chronic and multiple with the illness and suffering of the affected person numerous. The lack of cure for NCDs, the high cost of drugs, their high side-effects, and the emergence of multiple drug resistance has given rise to the investigation of other sources for therapeutic cure such as medicinal plants. The ethanol, n-hexane and ethyl acetate extracts of Olea europaea were analysed for their antioxidant and antimicrobial activities. The essential oil was also analysed for their chemical constituents. The n-hexane extracts of O. europaea exhibited no inhibition against all of the microorganisms tested, while the ethyl acetate and ethanol extracts exhibited inhibition, with minimum inhibitory concentration values between 0.625 mg/ml to 1.25 mg/ml. The ethanol leaf and ethyl acetate stem extracts exhibited significant activity in the inhibition of 2, 2-azinobis-(3-ethylbenzothiazolin - 6-sulfonic acid diammonium salt (ABTS) free radical, the n-hexane leaf extract had the overall significant lipid peroxidation inhibition activity, while in the inhibition of 2, 2- diphenyl-1-picrylhydrazyl radical (DPPH), the ethanol and ethyl acetate leaf extracts had strong activity. Nonanal, phytol, α-Pinene, α-Phellandrene, spatulenol and farnesol were some of chemical components identified after the GC-MS analysis of O. europaea oil. In the final part of the dissertation, Euryops brevipapposus essential oil was assessed for the antioxidant activities using free radical scavenging assays. In addition to this, the antimicrobial activities were assessed and the chemical composition was analysed using GC-MS. The essential oil demonstrated significant antioxidant activity against 2, 2-diphenyl-2-picryl-hydrazyl free radical (DPPH), 2, 2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and lipid peroxides with IC50 value of 0.0000000671 mg/ml, 1.05 mg/ml, and 1.170 mg/ml respectively. The essential oil also showed significant activity against all microorganisms tested with minimum inhibitory concentration (MIC) values between 0.055 mg/ml to 0.5 mg/ml. α-pinene, α- Phellandrene, germacrene D, β-pinene, trans- β.-Ocimene, bicyclogermacrene and β -Phellandrene were some of the chemical compounds identified in E. brevipapposus oil. The study has shown that E. brevipapposus and O. europaea are abundant in phytochemical compounds which were thought to be the root cause for the activities demonstrated. Therefore, these therapeutic properties observed validate and elucidate the traditional usage of the both plants in the treatment /management of diseases.
- Full Text:
- Date Issued: 2016
- Authors: Adegborioye, Abiodun
- Date: 2016
- Subjects: Antioxidants , Medicinal plants , Traditional medicine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4869 , vital:28624
- Description: Chronic non-communicable diseses are a global public health challenge that continuously threatens the development and health of humans. Risk factors such as unbalanced diet-the high consumption of processed food or food from animal origin are responsible for NCDs. NCDs result in weakened immune system, making the host susceptible to opportunistic infections. Thus, the NCDs burden is most times chronic and multiple with the illness and suffering of the affected person numerous. The lack of cure for NCDs, the high cost of drugs, their high side-effects, and the emergence of multiple drug resistance has given rise to the investigation of other sources for therapeutic cure such as medicinal plants. The ethanol, n-hexane and ethyl acetate extracts of Olea europaea were analysed for their antioxidant and antimicrobial activities. The essential oil was also analysed for their chemical constituents. The n-hexane extracts of O. europaea exhibited no inhibition against all of the microorganisms tested, while the ethyl acetate and ethanol extracts exhibited inhibition, with minimum inhibitory concentration values between 0.625 mg/ml to 1.25 mg/ml. The ethanol leaf and ethyl acetate stem extracts exhibited significant activity in the inhibition of 2, 2-azinobis-(3-ethylbenzothiazolin - 6-sulfonic acid diammonium salt (ABTS) free radical, the n-hexane leaf extract had the overall significant lipid peroxidation inhibition activity, while in the inhibition of 2, 2- diphenyl-1-picrylhydrazyl radical (DPPH), the ethanol and ethyl acetate leaf extracts had strong activity. Nonanal, phytol, α-Pinene, α-Phellandrene, spatulenol and farnesol were some of chemical components identified after the GC-MS analysis of O. europaea oil. In the final part of the dissertation, Euryops brevipapposus essential oil was assessed for the antioxidant activities using free radical scavenging assays. In addition to this, the antimicrobial activities were assessed and the chemical composition was analysed using GC-MS. The essential oil demonstrated significant antioxidant activity against 2, 2-diphenyl-2-picryl-hydrazyl free radical (DPPH), 2, 2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and lipid peroxides with IC50 value of 0.0000000671 mg/ml, 1.05 mg/ml, and 1.170 mg/ml respectively. The essential oil also showed significant activity against all microorganisms tested with minimum inhibitory concentration (MIC) values between 0.055 mg/ml to 0.5 mg/ml. α-pinene, α- Phellandrene, germacrene D, β-pinene, trans- β.-Ocimene, bicyclogermacrene and β -Phellandrene were some of the chemical compounds identified in E. brevipapposus oil. The study has shown that E. brevipapposus and O. europaea are abundant in phytochemical compounds which were thought to be the root cause for the activities demonstrated. Therefore, these therapeutic properties observed validate and elucidate the traditional usage of the both plants in the treatment /management of diseases.
- Full Text:
- Date Issued: 2016
Incidence and molecular characterization of Hepatitis E virus from swine herds in the Eastern Cape province, South Africa
- Authors: Adelabu, Olusesan Adeyemi
- Date: 2016
- Subjects: Hepatitis -- South Africa -- Eastern Cape Virology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/13966 , vital:39734
- Description: Hepatitis E virus-mediated infection is a serious public health concern in economically developing nations of the world. Globally, four major genotypes of HEV have been documented and the swine HEV identified to date in different countries belongs to either genotype 3 or 4. It has been reported to cross species barrier and cause infections in humans. Hence, Hepatitis E has been suggested to be zoonotic owing to the increase of evidence through various studies. Thus far, genotypes 1 and 2 are exclusively found in human population. However, little data are available regarding the occurrence of HEV in South Africa, especially in the Eastern Cape Province. Hence, this study was designed to assess the prevalence of Hepatitis E Virus among swine herd in selected communal and commercial farms in the Eastern Cape Province of South Africa. A total of 160 faecal samples were collected from swine herds in Amathole and Chris Hani District Municipalities of Eastern Cape Province and screened for the presence of HEV. Faecal samples were collected, using sterile swabs, from swine between the age of 2 to 6 months old, placed on ice and transported to the laboratory for further analysis. Viral RNA extraction was carried out followed by nested reverse-transcriptase polymerase chain reaction was used for amplification, after which the amplified products were examined by electrophoresis and viewed under ultraviolet illumination. The positive PCR products were sent for sequencing. Of the 160 faecal samples screened, only seven were positive (4.4percent) for HEV. The nucleotide sequence analyses revealed isolates UFT 01-03 sharing 82percent to 99percent identities with other strains with accession numbers KX896664, KX896665 and KX896666 and UMCF 01-04 with KX896667, KX896668, KX896669 and KX896670 from different regions of the world Phylogenetic analysis also showed that all seven isolates from this study clustered with both human and swine HEV from different geographical regions of the world especially with Japan Human and swine strains, Netherland swine HEV strains (AY032758, AF332620) and human HEV strain from France (JQ763611). We conclude that HEV is present among swine in the Eastern Cape Province, albeit in low incidence, and this does have public health implications. There is need for maintenance of high hygienic standards in order to prevent human infections through swine faecal materials and appropriate cooking of pork is highly advised.
- Full Text:
- Date Issued: 2016
- Authors: Adelabu, Olusesan Adeyemi
- Date: 2016
- Subjects: Hepatitis -- South Africa -- Eastern Cape Virology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/13966 , vital:39734
- Description: Hepatitis E virus-mediated infection is a serious public health concern in economically developing nations of the world. Globally, four major genotypes of HEV have been documented and the swine HEV identified to date in different countries belongs to either genotype 3 or 4. It has been reported to cross species barrier and cause infections in humans. Hence, Hepatitis E has been suggested to be zoonotic owing to the increase of evidence through various studies. Thus far, genotypes 1 and 2 are exclusively found in human population. However, little data are available regarding the occurrence of HEV in South Africa, especially in the Eastern Cape Province. Hence, this study was designed to assess the prevalence of Hepatitis E Virus among swine herd in selected communal and commercial farms in the Eastern Cape Province of South Africa. A total of 160 faecal samples were collected from swine herds in Amathole and Chris Hani District Municipalities of Eastern Cape Province and screened for the presence of HEV. Faecal samples were collected, using sterile swabs, from swine between the age of 2 to 6 months old, placed on ice and transported to the laboratory for further analysis. Viral RNA extraction was carried out followed by nested reverse-transcriptase polymerase chain reaction was used for amplification, after which the amplified products were examined by electrophoresis and viewed under ultraviolet illumination. The positive PCR products were sent for sequencing. Of the 160 faecal samples screened, only seven were positive (4.4percent) for HEV. The nucleotide sequence analyses revealed isolates UFT 01-03 sharing 82percent to 99percent identities with other strains with accession numbers KX896664, KX896665 and KX896666 and UMCF 01-04 with KX896667, KX896668, KX896669 and KX896670 from different regions of the world Phylogenetic analysis also showed that all seven isolates from this study clustered with both human and swine HEV from different geographical regions of the world especially with Japan Human and swine strains, Netherland swine HEV strains (AY032758, AF332620) and human HEV strain from France (JQ763611). We conclude that HEV is present among swine in the Eastern Cape Province, albeit in low incidence, and this does have public health implications. There is need for maintenance of high hygienic standards in order to prevent human infections through swine faecal materials and appropriate cooking of pork is highly advised.
- Full Text:
- Date Issued: 2016
Evaluating late payment-induced waste in South Africa through lean construction principles
- Authors: Akuffo-Ntow, Eric Ayesu
- Date: 2016
- Subjects: Construction industry -- South Africa -- Finance , Lean manufacturing -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/8867 , vital:26437
- Description: Central to the sustainable and competitive growth of the South African construction industry is a predictable cash flow through on time payment by clients to contractors. However, chronic late payments, a recurring problem in the industry, especially in the public sector, is bringing untold hardship to contractors by derailing their competitiveness and also eroding the trust among the supply chain partners to the extent that some firms end up closing business in the industry. The situation is viewed from the lean construction perspective as an obstacle that is preventing the accrual of the required value to project parties in the construction industry. This research addresses the causes of late payments to contractors and the role of the quantity surveyor (QS) in terms of influencing early payment from a lean construction perspective. A mixed method approach was used for compiling the primary data for the study. Sequentially, one hundred and two (102) quantitative and twelve (12) interview questionnaires were administered to selected contractors and construction professionals working predominantly on public sector projects. The findings revealed that the approval process for evaluation and certification by the client- appointed agent and intermediary institutions is taking longer than contractually allowed, with parties independently undertaking their evaluation prior to the certification date. This is found to be adverse to achieving consensus on the values of the works for authorisation. Other significant barriers are the lack of funds to authorise payment, random auditor general’s documentation changes, lack of administrators’ system integration and capacity to handle cyclical payment processing. Also, contractors have been found to be in default of timeously submitting complete claim documents. It is therefore recommended that the client agents should partner with the contractor in regular design and documentation reviews, arranging the payment process and should also meet jointly to prepare monthly evaluations. In addition, contractors should be mentored from the onset of the project regarding all the necessary documentation and supporting documents that will be required by the client to ensure that payment approvals are not delayed. The payment authori-sation agent’s capacity should be enhanced through training and improved powers as a singular point of control to perform efficiently and their systems and processes should be integrated to ensure that all parties are implementing the same protocols.
- Full Text:
- Date Issued: 2016
- Authors: Akuffo-Ntow, Eric Ayesu
- Date: 2016
- Subjects: Construction industry -- South Africa -- Finance , Lean manufacturing -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/8867 , vital:26437
- Description: Central to the sustainable and competitive growth of the South African construction industry is a predictable cash flow through on time payment by clients to contractors. However, chronic late payments, a recurring problem in the industry, especially in the public sector, is bringing untold hardship to contractors by derailing their competitiveness and also eroding the trust among the supply chain partners to the extent that some firms end up closing business in the industry. The situation is viewed from the lean construction perspective as an obstacle that is preventing the accrual of the required value to project parties in the construction industry. This research addresses the causes of late payments to contractors and the role of the quantity surveyor (QS) in terms of influencing early payment from a lean construction perspective. A mixed method approach was used for compiling the primary data for the study. Sequentially, one hundred and two (102) quantitative and twelve (12) interview questionnaires were administered to selected contractors and construction professionals working predominantly on public sector projects. The findings revealed that the approval process for evaluation and certification by the client- appointed agent and intermediary institutions is taking longer than contractually allowed, with parties independently undertaking their evaluation prior to the certification date. This is found to be adverse to achieving consensus on the values of the works for authorisation. Other significant barriers are the lack of funds to authorise payment, random auditor general’s documentation changes, lack of administrators’ system integration and capacity to handle cyclical payment processing. Also, contractors have been found to be in default of timeously submitting complete claim documents. It is therefore recommended that the client agents should partner with the contractor in regular design and documentation reviews, arranging the payment process and should also meet jointly to prepare monthly evaluations. In addition, contractors should be mentored from the onset of the project regarding all the necessary documentation and supporting documents that will be required by the client to ensure that payment approvals are not delayed. The payment authori-sation agent’s capacity should be enhanced through training and improved powers as a singular point of control to perform efficiently and their systems and processes should be integrated to ensure that all parties are implementing the same protocols.
- Full Text:
- Date Issued: 2016
The classsification of fuzzy subgroups of some finite Abelian p-groups of rank 3
- Authors: Appiah, Isaac Kwadwo
- Date: 2016
- Subjects: Fuzzy sets Abelian groups Finite groups
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2468 , vital:27845
- Description: An important trend in fuzzy group theory in recent years has been the notion of classification of fuzzy subgroups using a suitable equivalence relation. In this dissertation, we have successfully used the natural equivalence relation defined by Murali and Makamba in [81] and a natural fuzzy isomorphism to classify fuzzy subgroups of some finite abelian p-groups of rank three of the form Zpn + Zp + Zp for any fixed prime integer p and any positive integer n. This was achieved through the usage of a suitable technique of enumerating distinct fuzzy subgroups and non-isomorphic fuzzy subgroups of G. We commence by giving a brief discussion on the theory of fuzzy sets and fuzzy subgroups from the perspective of group theory through to the theory of sets, leading us to establish a linkage among these theories. We have also shown in this dissertation that the converse of theorem 3.1 proposed by Das in [24] is incorrect by giving a counter example and restate the theorem. We have then reviewed and enriched the study conducted by Ngcibi in [94] by characterising the non-isomorphic fuzzy subgroups in that study. We have also developed a formula to compute the crisp subgroups of the under-studied group and provide its proof. Furthermore, we have compared the equivalence relation under which the classification problem is based with various versions of equivalence studied in the literature. We managed to use this counting technique to obtain explicit formulae for the number of maximal chains, distinct fuzzy subgroups, non-isomorphic maximal chains and non-isomorphic fuzzy subgroups of these groups and their proofs are provided.
- Full Text:
- Date Issued: 2016
- Authors: Appiah, Isaac Kwadwo
- Date: 2016
- Subjects: Fuzzy sets Abelian groups Finite groups
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2468 , vital:27845
- Description: An important trend in fuzzy group theory in recent years has been the notion of classification of fuzzy subgroups using a suitable equivalence relation. In this dissertation, we have successfully used the natural equivalence relation defined by Murali and Makamba in [81] and a natural fuzzy isomorphism to classify fuzzy subgroups of some finite abelian p-groups of rank three of the form Zpn + Zp + Zp for any fixed prime integer p and any positive integer n. This was achieved through the usage of a suitable technique of enumerating distinct fuzzy subgroups and non-isomorphic fuzzy subgroups of G. We commence by giving a brief discussion on the theory of fuzzy sets and fuzzy subgroups from the perspective of group theory through to the theory of sets, leading us to establish a linkage among these theories. We have also shown in this dissertation that the converse of theorem 3.1 proposed by Das in [24] is incorrect by giving a counter example and restate the theorem. We have then reviewed and enriched the study conducted by Ngcibi in [94] by characterising the non-isomorphic fuzzy subgroups in that study. We have also developed a formula to compute the crisp subgroups of the under-studied group and provide its proof. Furthermore, we have compared the equivalence relation under which the classification problem is based with various versions of equivalence studied in the literature. We managed to use this counting technique to obtain explicit formulae for the number of maximal chains, distinct fuzzy subgroups, non-isomorphic maximal chains and non-isomorphic fuzzy subgroups of these groups and their proofs are provided.
- Full Text:
- Date Issued: 2016
Thermoluminescence of annealed synthetic quartz
- Atang, Elizabeth Fende Midiki
- Authors: Atang, Elizabeth Fende Midiki
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/420 , vital:19957
- Description: The kinetic and dosimetric features of the main thermoluminescent peak of synthetic quartz have been investigated in quartz ordinarily annealed at 500_C as well as quartz annealed at 500_C for 10 minutes. The main peak is found at 78 _C for the samples annealed at 500_C for 10 minutes irradiated to 10 Gy and heated at 1.0 _C/s. For the samples ordinarily annealed at 500_C the main peak is found at 106 _C after the sample has been irradiated to 30 Gy and heated at 5.0 _C/s. In these samples, the intensity of the main peak is enhanced with repetitive measurement whereas its maximum temperature is unaffected. The peak position of the main peak in the sample is independent of the irradiation dose and this, together with its fading characteristics, are consistent with first-order kinetics. For doses between 5 and 25 Gy, the dose response of the main peak of the annealed sample is superlinear. The half-life of the main TL peak of the annealed sample is about 1 h. The activation energy E of the main peak is around 0.90 eV. For a heating rate of 0.4 _C/s, its order of kinetics b derived from the whole curve method of analysis is 1.0. Following irradiation, preheating and illumination with 470 nm blue light, the main peak in the annealed sample is regenerated during heating. The resulting phototransferred peak occurs at the same temperature as the original peak and has similar kinetic and dosimetric features, with a half-life of about 1 h. For a preheat temperature of 200 _C, the intensity of the phototransferred peak in the sample increases with illumination time up to a maximum and decreases thereafter. At longer illumination times, no further decrease in the intensity of the phototransferred peak is observed. The traps associated with the 325 _C peak are the main source of the electrons responsible for the regenerated peak.
- Full Text:
- Date Issued: 2016
- Authors: Atang, Elizabeth Fende Midiki
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/420 , vital:19957
- Description: The kinetic and dosimetric features of the main thermoluminescent peak of synthetic quartz have been investigated in quartz ordinarily annealed at 500_C as well as quartz annealed at 500_C for 10 minutes. The main peak is found at 78 _C for the samples annealed at 500_C for 10 minutes irradiated to 10 Gy and heated at 1.0 _C/s. For the samples ordinarily annealed at 500_C the main peak is found at 106 _C after the sample has been irradiated to 30 Gy and heated at 5.0 _C/s. In these samples, the intensity of the main peak is enhanced with repetitive measurement whereas its maximum temperature is unaffected. The peak position of the main peak in the sample is independent of the irradiation dose and this, together with its fading characteristics, are consistent with first-order kinetics. For doses between 5 and 25 Gy, the dose response of the main peak of the annealed sample is superlinear. The half-life of the main TL peak of the annealed sample is about 1 h. The activation energy E of the main peak is around 0.90 eV. For a heating rate of 0.4 _C/s, its order of kinetics b derived from the whole curve method of analysis is 1.0. Following irradiation, preheating and illumination with 470 nm blue light, the main peak in the annealed sample is regenerated during heating. The resulting phototransferred peak occurs at the same temperature as the original peak and has similar kinetic and dosimetric features, with a half-life of about 1 h. For a preheat temperature of 200 _C, the intensity of the phototransferred peak in the sample increases with illumination time up to a maximum and decreases thereafter. At longer illumination times, no further decrease in the intensity of the phototransferred peak is observed. The traps associated with the 325 _C peak are the main source of the electrons responsible for the regenerated peak.
- Full Text:
- Date Issued: 2016
Construction project manager health and safety interventions towards improving workers' performance
- Authors: Ayessaki, Winn-Yam Houdou
- Date: 2016
- Subjects: Construction industry -- Management -- Health aspects Construction workers -- Health aspects -- South Africa Construction workers -- Safety measures
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/12275 , vital:27050
- Description: Optimum construction worker (CW) performance is required to achieve project delivery within project parameters. It is not always the case as CWs are regularly exposed to hazards, involved in accidents, their productivity is poor, they suffer from ill health, suffer from work-related musculoskeletal disorders (WMSDs) and contractors lack resources to allocate towards H&S. However, the lack or the absence of health and safety (H&S) measures, which the aforementioned depend on, has a negative impact on workers’ performance. Two descriptive surveys were conducted among professional construction project managers (CPMs) registered with the South African Council for Project and Construction Management Professions (SACPCMP) and general contractors (GCs) registered with the East Cape Master Builders Association (ECMBA). Interviews were also conducted with CPMs registered with the SACPCMP residing in the Nelson Mandela Bay metropolitan area. The salient findings include: • Accidents, which occur on construction sites, impact workers’ performance; • Inadequate H&S implementation affects both CW skills and motivation, which in turn affects their performance; • Poor constructability is relevant to H&S and CWs’ skills; • Provision and maintenance of welfare facilities are commonly inadequate and affect workers’ performance, and • Projects do not benefit from the adequate financial provision for H&S. It was concluded that exposure to hazards, poor site conditions, inadequate provision of welfare facilities, WMSDs, and insufficient financial provision for H&S affect workers’ performance by either incapacitating or demotivating them. Recommendations include : CPMs should make better use of their influence on clients; they should improve communication channels between project stakeholders; legislators need to raise awareness regarding H&S and worker welfare, and training and education institutions need to empower workers and professionals with H&S knowledge.
- Full Text:
- Date Issued: 2016
- Authors: Ayessaki, Winn-Yam Houdou
- Date: 2016
- Subjects: Construction industry -- Management -- Health aspects Construction workers -- Health aspects -- South Africa Construction workers -- Safety measures
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/12275 , vital:27050
- Description: Optimum construction worker (CW) performance is required to achieve project delivery within project parameters. It is not always the case as CWs are regularly exposed to hazards, involved in accidents, their productivity is poor, they suffer from ill health, suffer from work-related musculoskeletal disorders (WMSDs) and contractors lack resources to allocate towards H&S. However, the lack or the absence of health and safety (H&S) measures, which the aforementioned depend on, has a negative impact on workers’ performance. Two descriptive surveys were conducted among professional construction project managers (CPMs) registered with the South African Council for Project and Construction Management Professions (SACPCMP) and general contractors (GCs) registered with the East Cape Master Builders Association (ECMBA). Interviews were also conducted with CPMs registered with the SACPCMP residing in the Nelson Mandela Bay metropolitan area. The salient findings include: • Accidents, which occur on construction sites, impact workers’ performance; • Inadequate H&S implementation affects both CW skills and motivation, which in turn affects their performance; • Poor constructability is relevant to H&S and CWs’ skills; • Provision and maintenance of welfare facilities are commonly inadequate and affect workers’ performance, and • Projects do not benefit from the adequate financial provision for H&S. It was concluded that exposure to hazards, poor site conditions, inadequate provision of welfare facilities, WMSDs, and insufficient financial provision for H&S affect workers’ performance by either incapacitating or demotivating them. Recommendations include : CPMs should make better use of their influence on clients; they should improve communication channels between project stakeholders; legislators need to raise awareness regarding H&S and worker welfare, and training and education institutions need to empower workers and professionals with H&S knowledge.
- Full Text:
- Date Issued: 2016
Localizing selected endocytosis protein candidates in Plasmodium falciparum using GFP-tagged fusion constructs
- Authors: Basson, Travis
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2680 , vital:20316
- Description: Malaria is a mosquito-borne infectious disease caused by several obligate intracellular protozoan parasites in the Plasmodium genus, with Plasmodium falciparum causing the most widespread cases and malaria deaths. In 2013 there were approximately 190 million cases of the disease and between 584,000 and 855,000 deaths. It is essential to identify novel drug targets and develop novel drug candidates due to the increase in resistance of P. falciparum parasites to the current arsenal of antimalarial drugs. Endocytosis is an essential process in eukaryotic cells in which the external environment is internalized by the cell in order to obtain various particles from the extracellular space. This extracellular cytoplasm is internalized in membrane-bound invaginations at the plasma membrane. During the blood stage of malaria infection, the parasite requires nutrients from the host red blood cell. To obtain these nutrients, the parasite internalizes haemoglobin in large amounts and degrades it in an acidic, lysosome-like organelle, known as the digestive vacuole. Whilst the exact molecular mechanism of malaria parasite endocytosis is not yet fully understood, a number of proteins have been suggested to be involved. The most expedient approach in identifying candidate endocytosis proteins is to investigate parasite homologues of proteins known to be involved in endocytosis in mammalian cells. The three proteins selected for investigation in this study were the P. falciparum homologues of coronin, dynamin 2, and μ4. The coding sequences for the candidate endocytosis proteins were amplified by PCR and cloned into the pARL2-GFP expression vector. P. falciparum 3D7 parasites were transfected with these vectors and the episomal expression of full-length GFP-tagged fusion protein was confirmed by Western blot analysis using commercially available anti-GFP antibodies. Microscopic analysis of live parasites using fluorescence and confocal microscopy was used to determine the localization of the candidate endocytosis proteins. Coronin appeared to display diffuse cytoplasmic GFP localization during the trophozoite stage, arguing against a role in endocytosis. However, distinct localization during the schizont stage at what appears to be the inner membrane complex was observed. Coronin is thus likely required to coordinate the formation of the actin network between the merozoite IMC and the plasma membrane on which the glideosome is dependant for generating the motile forces required for the merozoite motility and invasion of RBCs. Dynamin 2 displayed localization at three potential locii: the parasite periphery (plasma membrane), punctuate regions within the cytoplasm (potentially at membrane bound organelles) and at the parasite food vacuole. The data suggested that dynamin 2 is involved in endocytosis and membrane trafficking in a similar manner to classical dynamins, potentially as a vesicle scission molecule at the plasma membrane, mediating vesicle formation at the food vacuole to recycle membrane to the plasma membrane, and possibly mitochondria organelle division. μ4 displayed transient localization, cycling between cytosolic localization, and localization to distinct regions at the plasma membrane and the food vacuole. Localization of Pfμ4 to the plasma membrane is indicative of a role for μ4 as a part of an adaptor protein (AP) complex which may be responsible for recruitment of clathrin to initiate endocytosis in a manner similar to mammalian AP-2. As was observed with PfDYN2, Pfμ4 localizes to the FV, which suggests that Pfμ4 forms part of a coat complex that mediates the formation of vesicles that recycle membrane from the FV to the parasite plasma membrane. This study showed that expressing proteins as full-length GFP-tagged fusion constructs is an effective approach in the early stages of determining the localization and function of P. falciparum proteins in vitro, and distinguishing between candidates that have a potential role in endocytosis and those that are unlikely to do so.
- Full Text:
- Date Issued: 2016
- Authors: Basson, Travis
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2680 , vital:20316
- Description: Malaria is a mosquito-borne infectious disease caused by several obligate intracellular protozoan parasites in the Plasmodium genus, with Plasmodium falciparum causing the most widespread cases and malaria deaths. In 2013 there were approximately 190 million cases of the disease and between 584,000 and 855,000 deaths. It is essential to identify novel drug targets and develop novel drug candidates due to the increase in resistance of P. falciparum parasites to the current arsenal of antimalarial drugs. Endocytosis is an essential process in eukaryotic cells in which the external environment is internalized by the cell in order to obtain various particles from the extracellular space. This extracellular cytoplasm is internalized in membrane-bound invaginations at the plasma membrane. During the blood stage of malaria infection, the parasite requires nutrients from the host red blood cell. To obtain these nutrients, the parasite internalizes haemoglobin in large amounts and degrades it in an acidic, lysosome-like organelle, known as the digestive vacuole. Whilst the exact molecular mechanism of malaria parasite endocytosis is not yet fully understood, a number of proteins have been suggested to be involved. The most expedient approach in identifying candidate endocytosis proteins is to investigate parasite homologues of proteins known to be involved in endocytosis in mammalian cells. The three proteins selected for investigation in this study were the P. falciparum homologues of coronin, dynamin 2, and μ4. The coding sequences for the candidate endocytosis proteins were amplified by PCR and cloned into the pARL2-GFP expression vector. P. falciparum 3D7 parasites were transfected with these vectors and the episomal expression of full-length GFP-tagged fusion protein was confirmed by Western blot analysis using commercially available anti-GFP antibodies. Microscopic analysis of live parasites using fluorescence and confocal microscopy was used to determine the localization of the candidate endocytosis proteins. Coronin appeared to display diffuse cytoplasmic GFP localization during the trophozoite stage, arguing against a role in endocytosis. However, distinct localization during the schizont stage at what appears to be the inner membrane complex was observed. Coronin is thus likely required to coordinate the formation of the actin network between the merozoite IMC and the plasma membrane on which the glideosome is dependant for generating the motile forces required for the merozoite motility and invasion of RBCs. Dynamin 2 displayed localization at three potential locii: the parasite periphery (plasma membrane), punctuate regions within the cytoplasm (potentially at membrane bound organelles) and at the parasite food vacuole. The data suggested that dynamin 2 is involved in endocytosis and membrane trafficking in a similar manner to classical dynamins, potentially as a vesicle scission molecule at the plasma membrane, mediating vesicle formation at the food vacuole to recycle membrane to the plasma membrane, and possibly mitochondria organelle division. μ4 displayed transient localization, cycling between cytosolic localization, and localization to distinct regions at the plasma membrane and the food vacuole. Localization of Pfμ4 to the plasma membrane is indicative of a role for μ4 as a part of an adaptor protein (AP) complex which may be responsible for recruitment of clathrin to initiate endocytosis in a manner similar to mammalian AP-2. As was observed with PfDYN2, Pfμ4 localizes to the FV, which suggests that Pfμ4 forms part of a coat complex that mediates the formation of vesicles that recycle membrane from the FV to the parasite plasma membrane. This study showed that expressing proteins as full-length GFP-tagged fusion constructs is an effective approach in the early stages of determining the localization and function of P. falciparum proteins in vitro, and distinguishing between candidates that have a potential role in endocytosis and those that are unlikely to do so.
- Full Text:
- Date Issued: 2016
Ecological principles for honeybush (Cyclopia spp.) conservation and cultivation
- Authors: Beyers, Andrea
- Date: 2016
- Subjects: Fynbos -- South Africa , Rooibos tea industry -- South Africa , Herbal teas -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/5723 , vital:20975
- Description: Market demand for the fynbos-derived honeybush (Cyclopia spp.) tea is on the rise, and there is an urgent need for better conservation of wild Cyclopia populations and for a shift to sustainable cultivation. Rooibos is another popular fynbos product that underwent exponential market growth over the last two decades but this resulted in large-scale conversion of pristine mountain fynbos to intensively-used crop lands. Adopting a conservation agriculture approach in expanding indigenous products will help in conserving biodiversity and ecosystem processes in this global biodiversity hotspot. Conservation agriculture of indigenous fynbos products grown in the Cape Floristic Region (CFR) should follow five main principles in order to maximise biodiversity conservation alongside agriculture. These principles are: 1) maintain high diversity at all levels, 2) conserve soil structure and processes, 3) conserve and restore natural ecosystem processes, 4) promote ecosystem stability and self-perpetuation, and 5) maintain natural areas as sources of ecosystem components. Given the negative ecological consequences of the industrialisation of the rooibos industry, it would be in the interest of biodiversity conservation, and even of agriculture, to apply these principles to honeybush cultivation. In order to prevent further fynbos habitat loss, cultivation of honeybush could be catalysed on old fields rather than in ploughed or cleared fynbos. Here I compare germination, growth and survival of two species (Cyclopia intermedia E.Mey. and C. subternata Vogel) between different land use types (ploughed lands, old fields and intact fynbos) and treatments (cleared and uncleared) in the Langkloof, Eastern Cape, over one year. Plots of each land use type were subject to each of these two treatments, although the ploughed plot constituted only a cleared treatment. Growth was highest on ploughed land, while survival and growth were lowest in uncleared fynbos. Cleared fynbos had the lowest germination success in both species, with the other sites and treatments coming out roughly the same. These results show that although ploughed lands give highest yields, growth and survival on old fields without ploughing is comparable with that on ploughed lands and is therefore a feasible alternative. Understanding the recruitment dynamics of honeybush is imperative for adequate conservation of wild populations. Furthermore, understanding this ecological aspect of the genus will help in developing the honeybush industry as a sustainable, agroecological market that uses semi-natural populations for production. Although Cyclopia species are early-successional species that germinate after fire, recruitment of Cyclopia pubescens Eckl. & Zeyh. continues throughout the inter-fire period. Cyclopia pubescens populations near Port Elizabeth were described according to their age structure and plant densities to discern possible density-dependent recruitment effects. Results showed that dense stands for the most part do not support high post-fire recruitment. This gives insight into suitable planting densities for new or restore populations and into management guidelines for remnant populations of threatened species. This thesis highlights important issues facing the honeybush industry. Mainstreaming biodiversity conservation into honeybush production is achievable, given that the industry is still relatively young, but this will require further research and strong market leadership.
- Full Text:
- Date Issued: 2016
- Authors: Beyers, Andrea
- Date: 2016
- Subjects: Fynbos -- South Africa , Rooibos tea industry -- South Africa , Herbal teas -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/5723 , vital:20975
- Description: Market demand for the fynbos-derived honeybush (Cyclopia spp.) tea is on the rise, and there is an urgent need for better conservation of wild Cyclopia populations and for a shift to sustainable cultivation. Rooibos is another popular fynbos product that underwent exponential market growth over the last two decades but this resulted in large-scale conversion of pristine mountain fynbos to intensively-used crop lands. Adopting a conservation agriculture approach in expanding indigenous products will help in conserving biodiversity and ecosystem processes in this global biodiversity hotspot. Conservation agriculture of indigenous fynbos products grown in the Cape Floristic Region (CFR) should follow five main principles in order to maximise biodiversity conservation alongside agriculture. These principles are: 1) maintain high diversity at all levels, 2) conserve soil structure and processes, 3) conserve and restore natural ecosystem processes, 4) promote ecosystem stability and self-perpetuation, and 5) maintain natural areas as sources of ecosystem components. Given the negative ecological consequences of the industrialisation of the rooibos industry, it would be in the interest of biodiversity conservation, and even of agriculture, to apply these principles to honeybush cultivation. In order to prevent further fynbos habitat loss, cultivation of honeybush could be catalysed on old fields rather than in ploughed or cleared fynbos. Here I compare germination, growth and survival of two species (Cyclopia intermedia E.Mey. and C. subternata Vogel) between different land use types (ploughed lands, old fields and intact fynbos) and treatments (cleared and uncleared) in the Langkloof, Eastern Cape, over one year. Plots of each land use type were subject to each of these two treatments, although the ploughed plot constituted only a cleared treatment. Growth was highest on ploughed land, while survival and growth were lowest in uncleared fynbos. Cleared fynbos had the lowest germination success in both species, with the other sites and treatments coming out roughly the same. These results show that although ploughed lands give highest yields, growth and survival on old fields without ploughing is comparable with that on ploughed lands and is therefore a feasible alternative. Understanding the recruitment dynamics of honeybush is imperative for adequate conservation of wild populations. Furthermore, understanding this ecological aspect of the genus will help in developing the honeybush industry as a sustainable, agroecological market that uses semi-natural populations for production. Although Cyclopia species are early-successional species that germinate after fire, recruitment of Cyclopia pubescens Eckl. & Zeyh. continues throughout the inter-fire period. Cyclopia pubescens populations near Port Elizabeth were described according to their age structure and plant densities to discern possible density-dependent recruitment effects. Results showed that dense stands for the most part do not support high post-fire recruitment. This gives insight into suitable planting densities for new or restore populations and into management guidelines for remnant populations of threatened species. This thesis highlights important issues facing the honeybush industry. Mainstreaming biodiversity conservation into honeybush production is achievable, given that the industry is still relatively young, but this will require further research and strong market leadership.
- Full Text:
- Date Issued: 2016
Investigating the role of Hsp90 and LRP1 in FN matrix dynamics
- Authors: Boël, Natasha Marie-Eraine
- Date: 2016
- Subjects: Extracellular matrix , Molecular chaperones , Heat shock proteins , Cancer , Fibronectins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2713 , vital:20319
- Description: Fibronectin (FN), a matrix protein responsible for regulating processes including migration and differentiation, is secreted as a soluble dimer which is assembled into an insoluble extracellular matrix. The dynamics of FN matrix assembly and degradation play a large role in cell migration and invasion contributing to the metastatic potential of cancer cells. Previous studies from our group have shown the direct binding of Hsp90 and FN in vitro and that inhibition of Hsp90 with novobiocin (NOV) caused internalisation of the FN matrix. However, the receptor mediating this internalisation is currently unknown. Low density lipoprotein 1 (LRP1) is a likely candidate as it is a ubiquitous receptor responsible for regulating internalisation of diverse ligands and is known to bind both Hsp90 and FN. We used wild type and knockout LRP1 cell lines to study the endocytosis of FN via this receptor. Here, we demonstrate that LRP1-deficient cells accumulated greatly increased levels of FN and were found to be less sensitive to pharmacological inhibition of Hsp90 by NOV. LRP1-expressing MEF-1 and Hs578T breast cancer cells experienced an increase in total FN in response to NOV, at concentrations below the EC50 value, followed by a dose-dependent loss of FN. We attributed greater FN levels to a loss of extracellular FN matrix coupled with increased internalisation of FN. Cell-surface biotinylation and DOC assays showed that loss of extracellular FN was specific to LRP1-expressing MEF-1 cells. Furthermore, we demonstrate that the loss of extracellular FN is not affected by changes in FN mRNA levels as determined by qRT-PCR, and that treatment with NOV resulted in the accelerated degradation of FN in the presence of cycloheximide. Immunoprecipitation studies reveal a putative complex exists between FN, Hsp90 and LRP1 in both cancer and non-cancer cells which is not perturbed by NOV. Western analyses revealed increased proteolytic processing of LRP1 in response to NOV which we proposed, based on literature, to modulate signalling pathways as a potential mechanism for regulating FN turnover. Moreover, using wound healing assays we identified increased migration to be one of the consequences associated with loss of extracellular FN by Hsp90 inhibition but only in cells containing LRP1. In summary, this study provides new insights into the Hsp90-LRP1 mediated loss of FN matrix and also reveals for the first time the functional consequence related to FN turnover by NOV was an increase in migration in LRP1-expressing cells.
- Full Text:
- Date Issued: 2016
- Authors: Boël, Natasha Marie-Eraine
- Date: 2016
- Subjects: Extracellular matrix , Molecular chaperones , Heat shock proteins , Cancer , Fibronectins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2713 , vital:20319
- Description: Fibronectin (FN), a matrix protein responsible for regulating processes including migration and differentiation, is secreted as a soluble dimer which is assembled into an insoluble extracellular matrix. The dynamics of FN matrix assembly and degradation play a large role in cell migration and invasion contributing to the metastatic potential of cancer cells. Previous studies from our group have shown the direct binding of Hsp90 and FN in vitro and that inhibition of Hsp90 with novobiocin (NOV) caused internalisation of the FN matrix. However, the receptor mediating this internalisation is currently unknown. Low density lipoprotein 1 (LRP1) is a likely candidate as it is a ubiquitous receptor responsible for regulating internalisation of diverse ligands and is known to bind both Hsp90 and FN. We used wild type and knockout LRP1 cell lines to study the endocytosis of FN via this receptor. Here, we demonstrate that LRP1-deficient cells accumulated greatly increased levels of FN and were found to be less sensitive to pharmacological inhibition of Hsp90 by NOV. LRP1-expressing MEF-1 and Hs578T breast cancer cells experienced an increase in total FN in response to NOV, at concentrations below the EC50 value, followed by a dose-dependent loss of FN. We attributed greater FN levels to a loss of extracellular FN matrix coupled with increased internalisation of FN. Cell-surface biotinylation and DOC assays showed that loss of extracellular FN was specific to LRP1-expressing MEF-1 cells. Furthermore, we demonstrate that the loss of extracellular FN is not affected by changes in FN mRNA levels as determined by qRT-PCR, and that treatment with NOV resulted in the accelerated degradation of FN in the presence of cycloheximide. Immunoprecipitation studies reveal a putative complex exists between FN, Hsp90 and LRP1 in both cancer and non-cancer cells which is not perturbed by NOV. Western analyses revealed increased proteolytic processing of LRP1 in response to NOV which we proposed, based on literature, to modulate signalling pathways as a potential mechanism for regulating FN turnover. Moreover, using wound healing assays we identified increased migration to be one of the consequences associated with loss of extracellular FN by Hsp90 inhibition but only in cells containing LRP1. In summary, this study provides new insights into the Hsp90-LRP1 mediated loss of FN matrix and also reveals for the first time the functional consequence related to FN turnover by NOV was an increase in migration in LRP1-expressing cells.
- Full Text:
- Date Issued: 2016
The EPR paradox: back from the future
- Authors: Bryan, Kate Louise Halse
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2881 , vital:20338
- Description: The Einstein-Podolsky-Rosen (EPR) thought experiment produced a problem regarding the interpretation of quantum mechanics provided for entangled systems. Although the thought experiment was reformulated mathematically in Bell's Theorem, the conclusion regarding entanglement correlations is still debated today. In an attempt to provide an explanation of how entangled systems maintain their correlations, this thesis investigates the theory of post-state teleportation as a possible interpretation of how information moves between entangled systems without resorting to nonlocal action. Post-state teleportation describes a method of communicating to the past via a quantum information channel. The resulting picture of the EPR thought experiment relied on information propagating backward from a final boundary condition to ensure all correlations were maintained. Similarities were found between this resolution of the EPR paradox and the final state solution to the black hole information paradox and the closely related firewall problem. The latter refers to an apparent conflict between unitary evaporation of a black hole and the strong subadditivity condition. The use of observer complementarity allows this solution of the black hole problem to be shown to be the same as a seemingly different solution known as “ER=EPR", where ‘ER’ refers to an Einstein-Rosen bridge or wormhole.
- Full Text:
- Date Issued: 2016
- Authors: Bryan, Kate Louise Halse
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2881 , vital:20338
- Description: The Einstein-Podolsky-Rosen (EPR) thought experiment produced a problem regarding the interpretation of quantum mechanics provided for entangled systems. Although the thought experiment was reformulated mathematically in Bell's Theorem, the conclusion regarding entanglement correlations is still debated today. In an attempt to provide an explanation of how entangled systems maintain their correlations, this thesis investigates the theory of post-state teleportation as a possible interpretation of how information moves between entangled systems without resorting to nonlocal action. Post-state teleportation describes a method of communicating to the past via a quantum information channel. The resulting picture of the EPR thought experiment relied on information propagating backward from a final boundary condition to ensure all correlations were maintained. Similarities were found between this resolution of the EPR paradox and the final state solution to the black hole information paradox and the closely related firewall problem. The latter refers to an apparent conflict between unitary evaporation of a black hole and the strong subadditivity condition. The use of observer complementarity allows this solution of the black hole problem to be shown to be the same as a seemingly different solution known as “ER=EPR", where ‘ER’ refers to an Einstein-Rosen bridge or wormhole.
- Full Text:
- Date Issued: 2016
An erosion and sediment delivery model for semi-arid catchments
- Authors: Bryson, Louise Kay
- Date: 2016
- Subjects: Sedimentation and deposition , Erosion , Watershed management -- South Africa , Water-supply -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6056 , http://hdl.handle.net/10962/d1020331
- Description: Sedimentation has become a significant environmental threat in South Africa as it intensifies water management problems in the water-scarce semi-arid regions of the country. As South Africa already allocates 98% of available water, the loss of storage capacity in reservoirs and degraded water quality has meant that a reliable water supply is compromised. The overall aim of this thesis was to develop a catchment scale model that represents the sediment dynamics of semi-arid regions of South Africa as a simple and practically applicable tool for water resource managers. Development of a conceptual framework for the model relied on an understanding of both the sediment dynamics of South African catchments and applicable modelling techniques. Scale was an issue in both cases as most of our understanding of the physical processes of runoff generation and sediment transport has been derived from plot scale studies. By identifying defining properties of semi-arid catchments it was possible to consider how temporal and spatial properties at higher levels emerged from properties at lower levels. These properties were effectively represented by using the Pitman rainfall-runoff model disaggregated to a daily timescale, the Modified Universal Soil Loss Equation (MUSLE) model incorporating probability function theory and through the representation of sediment storages across a semi-distributed catchment. The model was tested on two small and one large study catchment in the Karoo, South Africa, with limited observed data. Limitations to the model were found to be the large parameter data set and the dominance of structural constraints with an increase in catchment size. The next steps in model development will require a reduction of the parameter data set and an inclusion of an in-stream component for sub-catchments at a larger spatial scale. The model is applicable in areas such as South Africa where water resource managers need a simple model at the catchment scale in order to make decisions. This type of model provides a simple representation of the stochastic nature of erosion and sediment delivery over large spatial and temporal scales.
- Full Text:
- Date Issued: 2016
- Authors: Bryson, Louise Kay
- Date: 2016
- Subjects: Sedimentation and deposition , Erosion , Watershed management -- South Africa , Water-supply -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6056 , http://hdl.handle.net/10962/d1020331
- Description: Sedimentation has become a significant environmental threat in South Africa as it intensifies water management problems in the water-scarce semi-arid regions of the country. As South Africa already allocates 98% of available water, the loss of storage capacity in reservoirs and degraded water quality has meant that a reliable water supply is compromised. The overall aim of this thesis was to develop a catchment scale model that represents the sediment dynamics of semi-arid regions of South Africa as a simple and practically applicable tool for water resource managers. Development of a conceptual framework for the model relied on an understanding of both the sediment dynamics of South African catchments and applicable modelling techniques. Scale was an issue in both cases as most of our understanding of the physical processes of runoff generation and sediment transport has been derived from plot scale studies. By identifying defining properties of semi-arid catchments it was possible to consider how temporal and spatial properties at higher levels emerged from properties at lower levels. These properties were effectively represented by using the Pitman rainfall-runoff model disaggregated to a daily timescale, the Modified Universal Soil Loss Equation (MUSLE) model incorporating probability function theory and through the representation of sediment storages across a semi-distributed catchment. The model was tested on two small and one large study catchment in the Karoo, South Africa, with limited observed data. Limitations to the model were found to be the large parameter data set and the dominance of structural constraints with an increase in catchment size. The next steps in model development will require a reduction of the parameter data set and an inclusion of an in-stream component for sub-catchments at a larger spatial scale. The model is applicable in areas such as South Africa where water resource managers need a simple model at the catchment scale in order to make decisions. This type of model provides a simple representation of the stochastic nature of erosion and sediment delivery over large spatial and temporal scales.
- Full Text:
- Date Issued: 2016
Foraging ecology of the gentoo penguin, pygoscelis papua, at Marion Island
- Authors: Carpenter-Kling, Tegan
- Date: 2016
- Subjects: Gentoo penguin -- Food
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/4912 , vital:20766
- Description: The distribution and subsequent availability of marine predators’ prey is highly variable and is linked to fluctuating oceanographic parameters. It is well documented that annual breeding success of seabirds is related to the temporal availability of prey. Knowledge of a seabird’s diet and at sea distribution is therefore critical in deducing the effect of a fluctuating environment on a seabirds’ population and, furthermore, to understand seabirds role in the environment. The gentoo penguin population at sub-Antarctic Marion Island experienced a decline of 52% between 1994 and 2012. It has been speculated that long term changes in the local marine environment, due to a southward shift of the sub-Antarctic Front (SAF), and subsequent changes in prey availability is the ultimate causal factor. Therefore, this thesis investigates the temporal variation in the diet these birds over three years, through the use of stomach content samples. It then investigates whether the composition of the diet has changed over the long term (approximately 18 years). Additionally, the at sea distribution of these birds was investigated using temperature-depth recorders (TDRs) and global positioning system (GPS) devices. This study represents the first to investigate variation in the diet over three years and over the long term. It is also the first study to track these birds fine scale movement. Over three years, the diet exhibited a high degree of variability. The birds primarily foraged on the fish, Lepidonotothen larseni and the crustaceans, Nauticaris marionis and Euphausia vallentiniy. Over three years, L. larseni became increasingly more important during the breeding season. This was attributed to an increase in availability of these fish during this time, as this time corresponded to the transition of these fish from the larval pelagic phase to the benthic adult phase. Furthermore, the proportions of N. marionis and E. vallentini in the diet varied significantly among years. This variation is possibly a result of the highly variable latitudinal position of the SAF. The diet changed over the long term. There was significantly less N. marionis and more E. vallentini in the diet during more recent years. The long term southward shift of the SAF is considered to be a leading cause of these significant changes in the diet. The shallow inter-island shelf between Marion and Prince Edward Island was an important foraging area possibly due to predictable prey resources. In addition, these birds exhibited an important and possibly unique foraging strategy. They performed short self-maintenance trips where after they return to land to roost for the night. The following day these trips were followed by a longer foraging trip that was followed by chick provisioning. This strategy is unique among seabirds longer trips are associated with self-maintenance and not chick provisioning. This study has provided important knowledge of the foraging ecology of the gentoo penguin at sub-Antarctic Marion Island. It highlights the necessity for continuation of diet and tracking studies of these birds if we are to fully understand the ultimate factors that are causing the decline in their breeding success and population numbers.
- Full Text:
- Date Issued: 2016
- Authors: Carpenter-Kling, Tegan
- Date: 2016
- Subjects: Gentoo penguin -- Food
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/4912 , vital:20766
- Description: The distribution and subsequent availability of marine predators’ prey is highly variable and is linked to fluctuating oceanographic parameters. It is well documented that annual breeding success of seabirds is related to the temporal availability of prey. Knowledge of a seabird’s diet and at sea distribution is therefore critical in deducing the effect of a fluctuating environment on a seabirds’ population and, furthermore, to understand seabirds role in the environment. The gentoo penguin population at sub-Antarctic Marion Island experienced a decline of 52% between 1994 and 2012. It has been speculated that long term changes in the local marine environment, due to a southward shift of the sub-Antarctic Front (SAF), and subsequent changes in prey availability is the ultimate causal factor. Therefore, this thesis investigates the temporal variation in the diet these birds over three years, through the use of stomach content samples. It then investigates whether the composition of the diet has changed over the long term (approximately 18 years). Additionally, the at sea distribution of these birds was investigated using temperature-depth recorders (TDRs) and global positioning system (GPS) devices. This study represents the first to investigate variation in the diet over three years and over the long term. It is also the first study to track these birds fine scale movement. Over three years, the diet exhibited a high degree of variability. The birds primarily foraged on the fish, Lepidonotothen larseni and the crustaceans, Nauticaris marionis and Euphausia vallentiniy. Over three years, L. larseni became increasingly more important during the breeding season. This was attributed to an increase in availability of these fish during this time, as this time corresponded to the transition of these fish from the larval pelagic phase to the benthic adult phase. Furthermore, the proportions of N. marionis and E. vallentini in the diet varied significantly among years. This variation is possibly a result of the highly variable latitudinal position of the SAF. The diet changed over the long term. There was significantly less N. marionis and more E. vallentini in the diet during more recent years. The long term southward shift of the SAF is considered to be a leading cause of these significant changes in the diet. The shallow inter-island shelf between Marion and Prince Edward Island was an important foraging area possibly due to predictable prey resources. In addition, these birds exhibited an important and possibly unique foraging strategy. They performed short self-maintenance trips where after they return to land to roost for the night. The following day these trips were followed by a longer foraging trip that was followed by chick provisioning. This strategy is unique among seabirds longer trips are associated with self-maintenance and not chick provisioning. This study has provided important knowledge of the foraging ecology of the gentoo penguin at sub-Antarctic Marion Island. It highlights the necessity for continuation of diet and tracking studies of these birds if we are to fully understand the ultimate factors that are causing the decline in their breeding success and population numbers.
- Full Text:
- Date Issued: 2016
Historical perspectives and future directions for access to land, water and related ecosystem services in the Lower Sundays River Valley, South Africa: implications for human well being
- Authors: Chadzingwa, Karabo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/442 , vital:19959
- Description: The legacy of South Africa’s history has facilitated unequal access to land and water resources. In the Lower Sundays River Valley (LSRV), a predominantly commercial farming area, differential access to land and water has impacted aspects of ecosystem service access and human well‐being for disenfranchised families over time. Despite the social, political and economic reform efforts in the past two decades, severe inertia towards efforts attempting to increase equitable access to land and water has been experienced. As a result, communities have mobilized and claimed their land from the government through the land restitution process. Based on a mixed‐methods approach, this research explores the ways in which access to land and water over time has influenced current levels of human well‐being among disenfranchised families. Provisioning and cultural ecosystem services were identified as key areas of loss as a result of forced evictions from land. Freedom of choice was a central and cross‐cutting theme regarding the ability to change levels of human well‐being. Although the loss of ecosystem services associated with land and water had an impact on households, the ecosystem services which are regarded as fundamental to human well‐being do not seem to have been lost. The study recommends the wide use of freedom of choice as an indicator for well‐being in the LSRV, as well as a consideration of subjective, objective and psychological measures of well‐being with regards to natural resources and ecosystem services access. Key agents in fostering desirable pathways toward equity and sustainability in the LSRV are identified with stakeholders as private businesses; inclusive governance; empowered and skilled individuals, as well as NGOs.
- Full Text:
- Date Issued: 2016
- Authors: Chadzingwa, Karabo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/442 , vital:19959
- Description: The legacy of South Africa’s history has facilitated unequal access to land and water resources. In the Lower Sundays River Valley (LSRV), a predominantly commercial farming area, differential access to land and water has impacted aspects of ecosystem service access and human well‐being for disenfranchised families over time. Despite the social, political and economic reform efforts in the past two decades, severe inertia towards efforts attempting to increase equitable access to land and water has been experienced. As a result, communities have mobilized and claimed their land from the government through the land restitution process. Based on a mixed‐methods approach, this research explores the ways in which access to land and water over time has influenced current levels of human well‐being among disenfranchised families. Provisioning and cultural ecosystem services were identified as key areas of loss as a result of forced evictions from land. Freedom of choice was a central and cross‐cutting theme regarding the ability to change levels of human well‐being. Although the loss of ecosystem services associated with land and water had an impact on households, the ecosystem services which are regarded as fundamental to human well‐being do not seem to have been lost. The study recommends the wide use of freedom of choice as an indicator for well‐being in the LSRV, as well as a consideration of subjective, objective and psychological measures of well‐being with regards to natural resources and ecosystem services access. Key agents in fostering desirable pathways toward equity and sustainability in the LSRV are identified with stakeholders as private businesses; inclusive governance; empowered and skilled individuals, as well as NGOs.
- Full Text:
- Date Issued: 2016
Assessing the vulnerability of resource-poor households to disasters associated with climate variability using remote sensing and GIS techniques in the Nkonkobe Local Municipality, Eastern Cape Province, South Africa
- Authors: Chari, Martin Munashe
- Date: 2016
- Subjects: Climatic changes -- Forecasting Natural resources -- Remote sensing Droughts -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2425 , vital:27837
- Description: The main objective of the study was to assess the extent to which resource-poor households in selected villages of Nkonkobe Local Municipality in the Eastern Cape Province of South Africa are vulnerable to drought by using an improvised remote sensing and Geographic Information System (GIS)-based mapping approach. The research methodology was comprised of 1) assessment of vulnerability levels and 2) the calculation of established drought assessment indices comprising the Normalized Difference Vegetation Index (NDVI) and the Normalized Difference Water Index (NDWI) from wet-season Landsat images covering a period of 29 years from 1985 to 2014 in order to objectively determine the temporal recurrence of drought in Nkonkobe Local Municipality. Vulnerability of households to drought was determined by using a multi-step GIS-based mapping approach in which 3 components comprising exposure, sensitivity and adaptive capacity were simultaneously analysed and averaged to determine the magnitude of vulnerability. Thereafter, the Analytical Hierarchy Process (AHP) was used to establish weighted contributions of these components to vulnerability. The weights applied to the AHP were obtained from the 2012 - 2017 Nkonkobe Integrated Development Plan (IDP) and perceptions that were solicited from key informants who were judged to be knowledgeable about the subject. A Kruskal-Wallis H test on demographic data for water access revealed that the demographic results are independent of choice of data acquired from different data providers (χ2(2) = 1.26, p = 0.533, with a mean ranked population scores of 7.4 for ECSECC, 6.8 for Quantec and 9.8 for StatsSA). Simple linear regression analysis revealed strong positive correlations between NDWI and NDVI ((r = 0.99609375, R2 = 1, for 1985), 1995 (r = 0.99609375, R2 = 1 for 1995), (r = 0.99609375, R2 = 1 for 2005) and (r = 0.99609375, R2 = 1 for 2014). The regression analysis proved that vegetation condition depends on surface water arising from rainfall. The results indicate that the whole of Nkonkobe Local Municipality is susceptible to drought with villages in south eastern part being most vulnerable to droughts due to high sensitivity and low adaptive capacity.
- Full Text:
- Date Issued: 2016
- Authors: Chari, Martin Munashe
- Date: 2016
- Subjects: Climatic changes -- Forecasting Natural resources -- Remote sensing Droughts -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2425 , vital:27837
- Description: The main objective of the study was to assess the extent to which resource-poor households in selected villages of Nkonkobe Local Municipality in the Eastern Cape Province of South Africa are vulnerable to drought by using an improvised remote sensing and Geographic Information System (GIS)-based mapping approach. The research methodology was comprised of 1) assessment of vulnerability levels and 2) the calculation of established drought assessment indices comprising the Normalized Difference Vegetation Index (NDVI) and the Normalized Difference Water Index (NDWI) from wet-season Landsat images covering a period of 29 years from 1985 to 2014 in order to objectively determine the temporal recurrence of drought in Nkonkobe Local Municipality. Vulnerability of households to drought was determined by using a multi-step GIS-based mapping approach in which 3 components comprising exposure, sensitivity and adaptive capacity were simultaneously analysed and averaged to determine the magnitude of vulnerability. Thereafter, the Analytical Hierarchy Process (AHP) was used to establish weighted contributions of these components to vulnerability. The weights applied to the AHP were obtained from the 2012 - 2017 Nkonkobe Integrated Development Plan (IDP) and perceptions that were solicited from key informants who were judged to be knowledgeable about the subject. A Kruskal-Wallis H test on demographic data for water access revealed that the demographic results are independent of choice of data acquired from different data providers (χ2(2) = 1.26, p = 0.533, with a mean ranked population scores of 7.4 for ECSECC, 6.8 for Quantec and 9.8 for StatsSA). Simple linear regression analysis revealed strong positive correlations between NDWI and NDVI ((r = 0.99609375, R2 = 1, for 1985), 1995 (r = 0.99609375, R2 = 1 for 1995), (r = 0.99609375, R2 = 1 for 2005) and (r = 0.99609375, R2 = 1 for 2014). The regression analysis proved that vegetation condition depends on surface water arising from rainfall. The results indicate that the whole of Nkonkobe Local Municipality is susceptible to drought with villages in south eastern part being most vulnerable to droughts due to high sensitivity and low adaptive capacity.
- Full Text:
- Date Issued: 2016
Fatty acid composition, colour stability and lipid oxidation of mince produced from fresh and frozen/thawed fallow deer meat
- Authors: Chido, Chakanya
- Date: 2016
- Subjects: Lipids -- Oxidation Meat -- Preservation Mincemeat
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2479 , vital:27874
- Description: The aim of the study was to determine the fatty acid composition, colour stability and lipid oxidation of fresh mince produced from fallow deer and to evaluate the effect of frozen storage duration on the retail display shelf life of the mince. A total of 31 fallow deer carcasses were used in the study. After cooling for 24hrs, the carcasses were deboned, external fat from the fore and hindquarter muscles removed and individually vacuum packed. For the first trial, seven fallow deer carcasses were used. Meat from the hind and fore-quarters of each carcass was divided into two equal batches per animal. One batch was minced (through a 5 mm die) and packed into oxygen permeable overwraps and refrigerated at 4°C for a period of eight days under retail display conditions. The second batch was vacuum packed and frozen at -20°C for 2 months at the end of which mince was also produced and monitored over an eight-day period under the same conditions that were used for the fresh mince. Colour, pH, lipid and myoglobin stability was determined. Proximate and fatty acid composition was also determined. No differences (P>0.05) were noted between proximate composition of fresh and frozen/thawed minced meat. The lipid content of fallow deer was 2.4 percent (±0.04). Total n3 fatty acids differed (P<0.05) between treatments and decreased with increased storage and display day. There were significant (P<0.05) treatment and time interactions on all measured colour parameters, TBARS and myoglobin forms. Fresh mince was lighter and had higher redness (a*) and yellowness (b*) values than mince from two months frozen stored meat. Hue angle for fresh mince remained stable throughout display whereas it increased for frozen/thawed mince. Fresh mince had lower TBARS values than frozen/thawed mince. Minced meat produced from frozen/thawed deer meat had higher surface met-myoglobin and total met-myoglobin percentages. Surface and total oxy-myoglobin percentage was higher in fresh mince. The first trial clearly showed colour and lipid stability differences between fresh mince and mince from frozen/thawed meat. It also showed that fresh mince has a longer retail display life than mince produced from frozen/thawed meat (six days and four days, respectively). In the second trial, the effects of frozen storage duration on colour and lipid stability were investigated. Twenty-four fallow deer were used. Twelve were harvested in June (6male 6female) and the other twelve in August (6 male 6female) of the same year.Twenty four hours after harvesting, the fore and hindquarter muscles of the carcasses were deboned, vacuum packed and kept at -20°C until October (i.e. 2months and 4months frozen storage period). Upon thawing, the meat was processed into mince following the same procedure used for the first trialand displayed for a fiveday period under retail display conditions. Frozen duration and gender had no effect (P>0.05) on the proximate composition of fallow deer meat. The total amount of saturated fatty acids (SFA) increased and total amount of poly unsaturated fatty acids (PUFA) decreased as frozen duration and display day increased (P<0.05). Frozen duration affected (P<0.01) lipid oxidation and percentage oxy-myoglobin. Mince pH and all colour parameters (L*, a*, b*,hue and chroma) differed (P<0.05) between treatments on day zero and three. Display day was a significant factor (P<0.05) on all measured parameters. By day three all parameters except pH showed signs of extended oxidation and discolouration as evidenced by reduced redness, decreased colour intensity and high TBARS values. This study showed that prolonged frozen storage negatively affects the colour and lipid stability of meat and increases oxidation of PUFAs during frozen storage. However, the study also suggests that although frozen/thawed meat has a shorter retail display shelf life; the proximate composition of the meat remains unchanged.
- Full Text:
- Date Issued: 2016
- Authors: Chido, Chakanya
- Date: 2016
- Subjects: Lipids -- Oxidation Meat -- Preservation Mincemeat
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2479 , vital:27874
- Description: The aim of the study was to determine the fatty acid composition, colour stability and lipid oxidation of fresh mince produced from fallow deer and to evaluate the effect of frozen storage duration on the retail display shelf life of the mince. A total of 31 fallow deer carcasses were used in the study. After cooling for 24hrs, the carcasses were deboned, external fat from the fore and hindquarter muscles removed and individually vacuum packed. For the first trial, seven fallow deer carcasses were used. Meat from the hind and fore-quarters of each carcass was divided into two equal batches per animal. One batch was minced (through a 5 mm die) and packed into oxygen permeable overwraps and refrigerated at 4°C for a period of eight days under retail display conditions. The second batch was vacuum packed and frozen at -20°C for 2 months at the end of which mince was also produced and monitored over an eight-day period under the same conditions that were used for the fresh mince. Colour, pH, lipid and myoglobin stability was determined. Proximate and fatty acid composition was also determined. No differences (P>0.05) were noted between proximate composition of fresh and frozen/thawed minced meat. The lipid content of fallow deer was 2.4 percent (±0.04). Total n3 fatty acids differed (P<0.05) between treatments and decreased with increased storage and display day. There were significant (P<0.05) treatment and time interactions on all measured colour parameters, TBARS and myoglobin forms. Fresh mince was lighter and had higher redness (a*) and yellowness (b*) values than mince from two months frozen stored meat. Hue angle for fresh mince remained stable throughout display whereas it increased for frozen/thawed mince. Fresh mince had lower TBARS values than frozen/thawed mince. Minced meat produced from frozen/thawed deer meat had higher surface met-myoglobin and total met-myoglobin percentages. Surface and total oxy-myoglobin percentage was higher in fresh mince. The first trial clearly showed colour and lipid stability differences between fresh mince and mince from frozen/thawed meat. It also showed that fresh mince has a longer retail display life than mince produced from frozen/thawed meat (six days and four days, respectively). In the second trial, the effects of frozen storage duration on colour and lipid stability were investigated. Twenty-four fallow deer were used. Twelve were harvested in June (6male 6female) and the other twelve in August (6 male 6female) of the same year.Twenty four hours after harvesting, the fore and hindquarter muscles of the carcasses were deboned, vacuum packed and kept at -20°C until October (i.e. 2months and 4months frozen storage period). Upon thawing, the meat was processed into mince following the same procedure used for the first trialand displayed for a fiveday period under retail display conditions. Frozen duration and gender had no effect (P>0.05) on the proximate composition of fallow deer meat. The total amount of saturated fatty acids (SFA) increased and total amount of poly unsaturated fatty acids (PUFA) decreased as frozen duration and display day increased (P<0.05). Frozen duration affected (P<0.01) lipid oxidation and percentage oxy-myoglobin. Mince pH and all colour parameters (L*, a*, b*,hue and chroma) differed (P<0.05) between treatments on day zero and three. Display day was a significant factor (P<0.05) on all measured parameters. By day three all parameters except pH showed signs of extended oxidation and discolouration as evidenced by reduced redness, decreased colour intensity and high TBARS values. This study showed that prolonged frozen storage negatively affects the colour and lipid stability of meat and increases oxidation of PUFAs during frozen storage. However, the study also suggests that although frozen/thawed meat has a shorter retail display shelf life; the proximate composition of the meat remains unchanged.
- Full Text:
- Date Issued: 2016
Dynamic service orchestration in heterogeneous internet of things environments
- Authors: Chindenga, Edmore
- Date: 2016
- Subjects: Internet of things
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8001 , vital:31457
- Description: Internet of Things (IoT) presents a dynamic global revolution in the Internet where physical and virtual “things” will communicate and share information. As the number of devices increases, there is a need for a plug-and–interoperate approach of deploying “things” to the existing network with less or no human need for configuration. The plug-and-interoperate approach allows heterogeneous “things” to seamlessly interoperate, interact and exchange information and subsequently share services. Services are represented as functionalities that are offered by the “things”. Service orchestration provides an approach to integration and interoperability that decouples applications from each other, enhancing capabilities to centrally manage and monitor components. This work investigated requirements for semantic interoperability and exposed current challenges in IoT interoperability as a means of facilitating services orchestration in IoT. The research proposes a platform that allows heterogeneous devices to collaborate thereby enabling dynamic service orchestration. The platform provides a common framework for representing semantics allowing for a consistent information exchange format. The information is stored and presented in an ontology thereby preserving semantics and making the information comprehensible to machines allowing for automated addressing, tracking and discovery as well as information representation, storage, and exchange. Process mining techniques were used to discover service orchestrations. Process mining techniques enabled the analysis of runtime behavior of service orchestrations and the semantic breakdown of the service request and creation in real time. This enabled the research to draw observations that led to conclusions presented in this work. The research noted that the use of semantic technologies facilitates interoperability in heterogeneous devices and can be implemented as a means to bypass challenges presented by differences in IoT “things”.
- Full Text:
- Date Issued: 2016
- Authors: Chindenga, Edmore
- Date: 2016
- Subjects: Internet of things
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8001 , vital:31457
- Description: Internet of Things (IoT) presents a dynamic global revolution in the Internet where physical and virtual “things” will communicate and share information. As the number of devices increases, there is a need for a plug-and–interoperate approach of deploying “things” to the existing network with less or no human need for configuration. The plug-and-interoperate approach allows heterogeneous “things” to seamlessly interoperate, interact and exchange information and subsequently share services. Services are represented as functionalities that are offered by the “things”. Service orchestration provides an approach to integration and interoperability that decouples applications from each other, enhancing capabilities to centrally manage and monitor components. This work investigated requirements for semantic interoperability and exposed current challenges in IoT interoperability as a means of facilitating services orchestration in IoT. The research proposes a platform that allows heterogeneous devices to collaborate thereby enabling dynamic service orchestration. The platform provides a common framework for representing semantics allowing for a consistent information exchange format. The information is stored and presented in an ontology thereby preserving semantics and making the information comprehensible to machines allowing for automated addressing, tracking and discovery as well as information representation, storage, and exchange. Process mining techniques were used to discover service orchestrations. Process mining techniques enabled the analysis of runtime behavior of service orchestrations and the semantic breakdown of the service request and creation in real time. This enabled the research to draw observations that led to conclusions presented in this work. The research noted that the use of semantic technologies facilitates interoperability in heterogeneous devices and can be implemented as a means to bypass challenges presented by differences in IoT “things”.
- Full Text:
- Date Issued: 2016
The role of cancer procoagulant on the MTOR pathway
- Authors: Chiuswa, Chengetanai
- Date: 2016
- Subjects: Cancer -- Research , Neovascularization , Biochemistry , Blood coagulation factors -- Biotechnology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/7303 , vital:21316
- Description: Cancer procoagulant (CP) is a cysteine protease found in tumour cells and amnion chorion membranes. The main function of CP is not yet known, but it has potential roles in tumour growth and metastasis. Initially, CP was believed to increase coagulation in cancer patients; however, research has shown that increase in CP concentration does not correlate with an increase in coagulation. The location of CP in amnion chorion membranes and tumour cells only led to the hypothesis that CP might be involved in inducing blood vessel and or lymph vessel formation. CP was shown to induce lymphangiogenesis (lymph vessel formation) in human telomerase reverse transcriptase-human dermal endothelial cells (hTERT-HDLEC) (Tshaka, 2011). CP-induced tube formation was inhibited by rapamycin; indicating that CP may be signalling via the mammalian target of rapamycin (mTOR) pathway. The aim of this study was to investigate the effect of CP on the mTOR signalling pathway using human umbilical endothelial vein cells (HUVECs) as a model. CP was isolated from amnion chorion membranes and purified using two anion exchange chromatography steps. Purified CP (2 μg/ml) was used to induce tube formation in endothelial cells (HUVECs) seeded on growth factor reduced (GFR) Matrigel. In addition, the 2 μg/ml CP was used to treat cultured HUVECs. Sodium dodecyl sulphate polyacrylamide electrophoresis (SDS-PAGE) and western blotting were used to determine phosphorylation levels of protein kinase B (Akt) and ribosomal protein S6 kinase (S6K). CP was successfully isolated and purified using anion exchange chromatography. The effect of CP on tube formation was not significant relative to the control in the HUVEC cell line. The role of CP on Akt and S6K phosphorylation still needs to be verified by using sensitive methods of quantification such as enhanced chemiluminescence (ECL) and enzyme linked immunosorbent assay (ELISA). The levels of CP activity were shown to be higher in early tumour growth than in advanced cancer suggesting that certain physiological factors could be increasing CP activity during early tumour growth. This study investigated the effect of cobalt chloride on CP activity in breast cancer cell lines. Cobalt chloride reduced CP activity in MCF-7 and promoted CP activity in MDA-MB-231.
- Full Text:
- Date Issued: 2016
- Authors: Chiuswa, Chengetanai
- Date: 2016
- Subjects: Cancer -- Research , Neovascularization , Biochemistry , Blood coagulation factors -- Biotechnology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/7303 , vital:21316
- Description: Cancer procoagulant (CP) is a cysteine protease found in tumour cells and amnion chorion membranes. The main function of CP is not yet known, but it has potential roles in tumour growth and metastasis. Initially, CP was believed to increase coagulation in cancer patients; however, research has shown that increase in CP concentration does not correlate with an increase in coagulation. The location of CP in amnion chorion membranes and tumour cells only led to the hypothesis that CP might be involved in inducing blood vessel and or lymph vessel formation. CP was shown to induce lymphangiogenesis (lymph vessel formation) in human telomerase reverse transcriptase-human dermal endothelial cells (hTERT-HDLEC) (Tshaka, 2011). CP-induced tube formation was inhibited by rapamycin; indicating that CP may be signalling via the mammalian target of rapamycin (mTOR) pathway. The aim of this study was to investigate the effect of CP on the mTOR signalling pathway using human umbilical endothelial vein cells (HUVECs) as a model. CP was isolated from amnion chorion membranes and purified using two anion exchange chromatography steps. Purified CP (2 μg/ml) was used to induce tube formation in endothelial cells (HUVECs) seeded on growth factor reduced (GFR) Matrigel. In addition, the 2 μg/ml CP was used to treat cultured HUVECs. Sodium dodecyl sulphate polyacrylamide electrophoresis (SDS-PAGE) and western blotting were used to determine phosphorylation levels of protein kinase B (Akt) and ribosomal protein S6 kinase (S6K). CP was successfully isolated and purified using anion exchange chromatography. The effect of CP on tube formation was not significant relative to the control in the HUVEC cell line. The role of CP on Akt and S6K phosphorylation still needs to be verified by using sensitive methods of quantification such as enhanced chemiluminescence (ECL) and enzyme linked immunosorbent assay (ELISA). The levels of CP activity were shown to be higher in early tumour growth than in advanced cancer suggesting that certain physiological factors could be increasing CP activity during early tumour growth. This study investigated the effect of cobalt chloride on CP activity in breast cancer cell lines. Cobalt chloride reduced CP activity in MCF-7 and promoted CP activity in MDA-MB-231.
- Full Text:
- Date Issued: 2016
An insight into the profile characteristics and technical batting skill of adolescent cricket players
- Authors: Clark, Matthew Edward
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2838 , vital:20331
- Description: Background: Current traditional cricket practices incorporate the use of a netted-off pitch to provide cricket players the opportunity to hone their skills with bat and ball. The lack of ecological validity of these training environments results in the absence of important task and environmental constraints which influences the manner in which the sport is played. Objectives: The purpose of the current research was two-fold: firstly, it aimed to establish a profile database of adolescent cricket players in the Eastern Cape province of South Africa; and secondly, it aimed to investigate the efficacy of a constraints-led training intervention on skill development in adolescent cricket batsmen. Methods: Study I required 90 participants (u13: n = 40; u15: n = 50) to perform measures pertaining to anthropometric, morphological, flexibility and physical performance characteristics. This included stature, mass, body composition, limb length, flexibility, agility, and power measures. Study II was a case-control study (u15: n = 24), whereby the experimental group (n = 12) was exposed to a constraints-led batting protocol during the course of the cricket season. The objective of the protocol was to encourage the manipulation of the ball around the playing field. Pre- and post-intervention measures were performed using a batting skills test, which assessed batsmen’s capability to manipulate the ball to various areas of the playing field. Results: Study I revealed significant differences (p <0.0001) in stature, mass, limb length and power variables when comparing the u15 age group with the u13 age group. No differences were recorded for agility. The u13 age group had greater linear correlations for the variables of interest compared to the u15 age group. Study II revealed no differences in technical skill between the experimental group and the control (p <0.315).. The tests also revealed that adolescent batsmen favoured hitting deliveries through the extra-cover scoring zone. The third-man and fine-leg scoring zones were least preferred to hit deliveries through. A number of limitations to the study design resulted in the inability to determine the efficacy of a constraints-led batting protocol on skill development in an adolescent batting cohort. Conclusion: The results of study I indicated that the onset of puberty played an important role in the measures and performances between the u13 and u15 age groups. The results of study II indicated that adolescent batsmen are not adept at manipulating the ball around the field. Recommendations are provided for both studies.
- Full Text:
- Date Issued: 2016
- Authors: Clark, Matthew Edward
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2838 , vital:20331
- Description: Background: Current traditional cricket practices incorporate the use of a netted-off pitch to provide cricket players the opportunity to hone their skills with bat and ball. The lack of ecological validity of these training environments results in the absence of important task and environmental constraints which influences the manner in which the sport is played. Objectives: The purpose of the current research was two-fold: firstly, it aimed to establish a profile database of adolescent cricket players in the Eastern Cape province of South Africa; and secondly, it aimed to investigate the efficacy of a constraints-led training intervention on skill development in adolescent cricket batsmen. Methods: Study I required 90 participants (u13: n = 40; u15: n = 50) to perform measures pertaining to anthropometric, morphological, flexibility and physical performance characteristics. This included stature, mass, body composition, limb length, flexibility, agility, and power measures. Study II was a case-control study (u15: n = 24), whereby the experimental group (n = 12) was exposed to a constraints-led batting protocol during the course of the cricket season. The objective of the protocol was to encourage the manipulation of the ball around the playing field. Pre- and post-intervention measures were performed using a batting skills test, which assessed batsmen’s capability to manipulate the ball to various areas of the playing field. Results: Study I revealed significant differences (p <0.0001) in stature, mass, limb length and power variables when comparing the u15 age group with the u13 age group. No differences were recorded for agility. The u13 age group had greater linear correlations for the variables of interest compared to the u15 age group. Study II revealed no differences in technical skill between the experimental group and the control (p <0.315).. The tests also revealed that adolescent batsmen favoured hitting deliveries through the extra-cover scoring zone. The third-man and fine-leg scoring zones were least preferred to hit deliveries through. A number of limitations to the study design resulted in the inability to determine the efficacy of a constraints-led batting protocol on skill development in an adolescent batting cohort. Conclusion: The results of study I indicated that the onset of puberty played an important role in the measures and performances between the u13 and u15 age groups. The results of study II indicated that adolescent batsmen are not adept at manipulating the ball around the field. Recommendations are provided for both studies.
- Full Text:
- Date Issued: 2016
Population assessment and feeding ecology of brown hyenas (hyaena brunnea) in Mountain Zebra National Park, Eastern Cape, South Africa
- Authors: Comley, Jessica
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2862 , vital:20336
- Description: The development of many small (<400 km2), enclosed game reserves in the Eastern Cape Province of South Africa over the past 25 years has contributed greatly to the conservation of large carnivores. However, the brown hyena (Hyaena brunnea) is one of the least studied large carnivores in South Africa. Nevertheless, the reintroduction of this species (n=3 in 2008) into Mountain Zebra National Park (MZNP) provided the perfect opportunity to broaden our understanding of the role that this carnivore plays in an enclosed system. Camera trap data was collected for just over a year from April 2014 to April 2015 and brown hyena density estimates were calculated using spatially explicit capture-recapture analysis. Left-side images of brown hyenas were used in the analysis and 12 individuals were positively identified. The best model to estimate brown hyena density included a road covariate and estimated brown hyena density to be 6-10 individuals/100 km2 (an absolute abundance of between 12 and 21 individuals), which is higher than densities calculated for brown hyenas in other arid, open systems. In, addition, brown hyena scat samples were collected over a five year period from April 2011 to June 2015 and standard techniques for scat analysis were used to identify prey items. Cheetah (Acinonyx jubatus) and lion (Panthera leo) kill site data were used to investigate the impacts of these species on the diet of brown hyenas. Before the release of lions brown hyenas predominantly scavenged on medium-sized mammals, which was what the cheetahs mainly killed. However, after the release of the lions, brown hyenas predominantly scavenged on large mammals, which was what the lions primarily killed. The results from my study indicate that brown hyenas are most likely reaching high densities in enclosed systems, due to increased scavenging opportunities provided by other large predators. The rapid increase of brown hyena densities from small founder populations in enclosed reserves could result in inbreeding. Therefore, in order to successfully conserve brown hyenas and other large carnivores in South Africa, continual post-release monitoring and possible implementation of meta-population management schemes is required.
- Full Text:
- Date Issued: 2016
- Authors: Comley, Jessica
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2862 , vital:20336
- Description: The development of many small (<400 km2), enclosed game reserves in the Eastern Cape Province of South Africa over the past 25 years has contributed greatly to the conservation of large carnivores. However, the brown hyena (Hyaena brunnea) is one of the least studied large carnivores in South Africa. Nevertheless, the reintroduction of this species (n=3 in 2008) into Mountain Zebra National Park (MZNP) provided the perfect opportunity to broaden our understanding of the role that this carnivore plays in an enclosed system. Camera trap data was collected for just over a year from April 2014 to April 2015 and brown hyena density estimates were calculated using spatially explicit capture-recapture analysis. Left-side images of brown hyenas were used in the analysis and 12 individuals were positively identified. The best model to estimate brown hyena density included a road covariate and estimated brown hyena density to be 6-10 individuals/100 km2 (an absolute abundance of between 12 and 21 individuals), which is higher than densities calculated for brown hyenas in other arid, open systems. In, addition, brown hyena scat samples were collected over a five year period from April 2011 to June 2015 and standard techniques for scat analysis were used to identify prey items. Cheetah (Acinonyx jubatus) and lion (Panthera leo) kill site data were used to investigate the impacts of these species on the diet of brown hyenas. Before the release of lions brown hyenas predominantly scavenged on medium-sized mammals, which was what the cheetahs mainly killed. However, after the release of the lions, brown hyenas predominantly scavenged on large mammals, which was what the lions primarily killed. The results from my study indicate that brown hyenas are most likely reaching high densities in enclosed systems, due to increased scavenging opportunities provided by other large predators. The rapid increase of brown hyena densities from small founder populations in enclosed reserves could result in inbreeding. Therefore, in order to successfully conserve brown hyenas and other large carnivores in South Africa, continual post-release monitoring and possible implementation of meta-population management schemes is required.
- Full Text:
- Date Issued: 2016
Risk analysis and management systems in South African construction project management practices
- Authors: Cook, Iain Murray
- Date: 2016
- Subjects: Construction projects -- Management , Construction industry -- Risk management , Project management -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/3469 , vital:20433
- Description: Risk management (RM) should be seen as one of the most important functions in the South African built environment. Without the effective management of the risks associated with the industry, the noble vision of a sector that is efficient, profitable, and sustainable cannot be achieved. By embracing tried and tested policies that successfully mitigate risk, industry stakeholders will achieve many project successes, and will outlast any competitors that choose to ignore, or are ignorant of the fact, that the negative impact risk has on projects is inversely proportional to the level of RM employed. Construction Project Management (CPM) practices, realising that there are excellent business opportunities across South Africa’s borders, and faced with a competitive South African market, are engaging with developers and government entities involved in cross border projects in the hope of securing these potentially lucrative African projects. With this move into Africa comes increased uncertainty and risk for these CPM practices, and other project stakeholders. Similarly, CPM practices that have made the strategic decision to remain operational only within South Africa’s borders, are faced with a competitive and complex built environment and industry, made increasingly challenging by a weakening economy, exacerbated by industrial strikes, infrastructure deficiencies and a decrease in industry skill levels. This study reports on Project Managers’ (PMs’) perceptions of project failures and inefficiencies resulting from inadequate RM on projects, including the RM methodologies currently being employed. The study focused on perceptions of PMs who operate within South Africa’s borders, PMs that operate across border into other African countries, as well as PMs who operate exclusively within South Africa’s built environment framework. A study was undertaken incorporating qualitative methodologies via a normative survey. The survey was split into three main phases. Phase one employed the use of a pilot survey executed with the objective of further investigating the main sub-problems to gain more insight into the related issues and challenges. For the pilot survey, PMs were selected based on their engagement in CPM activities within South Africa as well as across South Africa’s borders into other African countries. Phase two of the main survey, with the sample stratum being the Association of Construction Project Managers (ACPM), was aimed at PMs within the ACPM who have engaged, or are engaging, in CPM activities both within South Africa’s borders as well as across South Africa’s borders into other African countries. Phase 3 of the main survey, with the sample stratum being the ACPM, was aimed at PMs within the ACPM who have engaged, or are engaging, in CPM activities within South Africa’s borders only and have not engaged in cross border activities. Survey findings identified the commercial sector and value of the projects undertaken by the practices, the level of risk associated with different client typologies, the link between inadequate RM and project inefficiency and failure, and the importance of RM on projects. Findings also identified that RM methodologies are employed by CPM practices, and that CPM practices generally endeavour to create a culture of risk awareness amongst employees. Further findings indicated that CPM practices may not always understand the risks associated with new industry sectors, regions or countries that they are considering operating within, and that that there is room for improvement regarding the effectiveness of current RM systems. Survey findings also indicated that risk is not always transferred to the correct project stakeholder most suited to managing the risk, and CPM practices are not always able to accurately quantify the costs associated with project risk. Furthermore, it was identified that CPM practices do not always undertaken risk assessments (RAs) at the correct project stage resulting in inadequate risk contingencies allowances, regular risk reviews are not always undertaken for projects, project pre-mortems are seen as valuable tools by CPM practices as a method to reduce future risk, and project post-mortems relative to ‘lessons learnt’ are not always undertaken. Conclusions outline the link between effective RM, project inefficiencies and project failure, as well as the increase or decrease in risk relative to ineffective or effective use of risk identification and management methodologies for time, cost, and quality factors respectively. Conclusions also outline the fact that although CPM practices generally understand the link between RM and project success, they are not always able to fully comprehend the risks associated with new industry sectors, regions or cross border countries. This indicates that without the adequate identification of risk, the RM process or steps that follow the qualitative risk identification process will have little or no value. This is indicative of the requirement for professional associations to consolidate risk data for industry activities with the aim of improving the level of RM industry wide. Recommendations highlight the importance of the compiling of sector specific risk registers, compiled by the South African Council for the Project and Construction Management Professions (SACPCMP) with registered member input, made available to all PMs via the SACPCMPs online database. Further recommendations include: the engendering, by senior management of CPM practices; a healthy ‘risk aware’ culture, by promoting RM practices aligned with best practice methodologies; the implementation of well balanced and formal RM systems throughout the CPM practice, with the aim of achieving effective RM without overburdening PMs with unnecessary documentation or ‘paperwork’; the attendance of risk conferences and workshops by all CPM practices, aimed at specifically identifying challenges that exist with RM and methods that can be employed to improve the status quo; the attendance of formal risk training courses, by all CPM practices, aimed at improving the knowledge base of PMs relative to effective RM, and the appointment of risk professionals, driven by the monetary value and risk levels of the project, to undertake the RM process and unburden PMs from the task, allowing PMs to concentrate on the other project knowledge areas.
- Full Text:
- Date Issued: 2016
- Authors: Cook, Iain Murray
- Date: 2016
- Subjects: Construction projects -- Management , Construction industry -- Risk management , Project management -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/3469 , vital:20433
- Description: Risk management (RM) should be seen as one of the most important functions in the South African built environment. Without the effective management of the risks associated with the industry, the noble vision of a sector that is efficient, profitable, and sustainable cannot be achieved. By embracing tried and tested policies that successfully mitigate risk, industry stakeholders will achieve many project successes, and will outlast any competitors that choose to ignore, or are ignorant of the fact, that the negative impact risk has on projects is inversely proportional to the level of RM employed. Construction Project Management (CPM) practices, realising that there are excellent business opportunities across South Africa’s borders, and faced with a competitive South African market, are engaging with developers and government entities involved in cross border projects in the hope of securing these potentially lucrative African projects. With this move into Africa comes increased uncertainty and risk for these CPM practices, and other project stakeholders. Similarly, CPM practices that have made the strategic decision to remain operational only within South Africa’s borders, are faced with a competitive and complex built environment and industry, made increasingly challenging by a weakening economy, exacerbated by industrial strikes, infrastructure deficiencies and a decrease in industry skill levels. This study reports on Project Managers’ (PMs’) perceptions of project failures and inefficiencies resulting from inadequate RM on projects, including the RM methodologies currently being employed. The study focused on perceptions of PMs who operate within South Africa’s borders, PMs that operate across border into other African countries, as well as PMs who operate exclusively within South Africa’s built environment framework. A study was undertaken incorporating qualitative methodologies via a normative survey. The survey was split into three main phases. Phase one employed the use of a pilot survey executed with the objective of further investigating the main sub-problems to gain more insight into the related issues and challenges. For the pilot survey, PMs were selected based on their engagement in CPM activities within South Africa as well as across South Africa’s borders into other African countries. Phase two of the main survey, with the sample stratum being the Association of Construction Project Managers (ACPM), was aimed at PMs within the ACPM who have engaged, or are engaging, in CPM activities both within South Africa’s borders as well as across South Africa’s borders into other African countries. Phase 3 of the main survey, with the sample stratum being the ACPM, was aimed at PMs within the ACPM who have engaged, or are engaging, in CPM activities within South Africa’s borders only and have not engaged in cross border activities. Survey findings identified the commercial sector and value of the projects undertaken by the practices, the level of risk associated with different client typologies, the link between inadequate RM and project inefficiency and failure, and the importance of RM on projects. Findings also identified that RM methodologies are employed by CPM practices, and that CPM practices generally endeavour to create a culture of risk awareness amongst employees. Further findings indicated that CPM practices may not always understand the risks associated with new industry sectors, regions or countries that they are considering operating within, and that that there is room for improvement regarding the effectiveness of current RM systems. Survey findings also indicated that risk is not always transferred to the correct project stakeholder most suited to managing the risk, and CPM practices are not always able to accurately quantify the costs associated with project risk. Furthermore, it was identified that CPM practices do not always undertaken risk assessments (RAs) at the correct project stage resulting in inadequate risk contingencies allowances, regular risk reviews are not always undertaken for projects, project pre-mortems are seen as valuable tools by CPM practices as a method to reduce future risk, and project post-mortems relative to ‘lessons learnt’ are not always undertaken. Conclusions outline the link between effective RM, project inefficiencies and project failure, as well as the increase or decrease in risk relative to ineffective or effective use of risk identification and management methodologies for time, cost, and quality factors respectively. Conclusions also outline the fact that although CPM practices generally understand the link between RM and project success, they are not always able to fully comprehend the risks associated with new industry sectors, regions or cross border countries. This indicates that without the adequate identification of risk, the RM process or steps that follow the qualitative risk identification process will have little or no value. This is indicative of the requirement for professional associations to consolidate risk data for industry activities with the aim of improving the level of RM industry wide. Recommendations highlight the importance of the compiling of sector specific risk registers, compiled by the South African Council for the Project and Construction Management Professions (SACPCMP) with registered member input, made available to all PMs via the SACPCMPs online database. Further recommendations include: the engendering, by senior management of CPM practices; a healthy ‘risk aware’ culture, by promoting RM practices aligned with best practice methodologies; the implementation of well balanced and formal RM systems throughout the CPM practice, with the aim of achieving effective RM without overburdening PMs with unnecessary documentation or ‘paperwork’; the attendance of risk conferences and workshops by all CPM practices, aimed at specifically identifying challenges that exist with RM and methods that can be employed to improve the status quo; the attendance of formal risk training courses, by all CPM practices, aimed at improving the knowledge base of PMs relative to effective RM, and the appointment of risk professionals, driven by the monetary value and risk levels of the project, to undertake the RM process and unburden PMs from the task, allowing PMs to concentrate on the other project knowledge areas.
- Full Text:
- Date Issued: 2016