Beauty from Ashes:short term postfire regeneration of dune fynbos -thicket vegetation in the South Eastern Cape floristic region
- Authors: Gcina, Sinenjongo
- Date: 2022-12
- Subjects: Fynbos ecology – Nelson Mandela University Campus Nature Reserve , Forest fires -- Prevention and control
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59666 , vital:62319
- Description: Aim This study aims to provide an understanding and a description of the short-term postfire regeneration and ecology of the fynbos-thicket vegetation on a coastal dune system in the southeastern Cape Floristic Region. Another aim is to assess the recovery of the vegetation community by assessing the growth forms and the disturbance response across a range of species from thicket and fynbos communities and to provide a belowground bud bank classification for each of these species in the coastal dune systems. Location The study site was the Mandela University Reserve and the Noordhoek dune fields, situated in Gqeberha, Eastern Cape, which is part of the south-eastern Cape Floristic Region. The study site hosts thicket-fynbos mosaic vegetation type that occurs in a Mediterranean-Climate Ecosystem (MCE). Methods Firstly, field observations and photographs of plant species were taken weekly to assess and identify the species that were emerging and persisting in the dune system in the first 12 months postfire. Phenological events of the species that emerged (e.g., flowering, fruiting, leaf growth, leaf yellowing, and leaf abscission) were also observed and reported on. Secondly, an excavation of 21 species (e.g., Jamesbrittenia microphylla, Euclea racemosa, Olea exasperata, Searsia laevigata) was conducted to classify the species into their belowground bud bank types. Results There was a rapid emergence of resprouters (facultative resprouters and obligate resprouters) in the first 12 months postfire. This was followed by their growth; primarily occurring after the rainfall events. The dominant resprouting species (facultative resprouters and obligate resprouters) entered the system two weeks after the fire. There was a significant difference in the time to the first emergence of the different regeneration groups postfire (P < 0.001): on average, obligate sprouters (OS) appeared 1 month after fire – two months earlier than facultative sprouters (FS) and four months earlier than non-sprouters (NS). There was no statistical evidence to suggest that the FS and non-sprouters NS emerged at different times postfire; however, most NS species were observed in the system 5 months after fire. 4 Exceptions were the NS species Pelargonium grossularoides and Mesembryanthemum aitonis, both annuals that emerged 1 month after fire. A wide range of OS species that appeared earlier in the system (e.g., Euclea racemosa, Olea exasperata, Searsia laevigata, and Lauridia tetragona) exhibit underground lateral growth with belowground bud banks (BBBs) from which they recover after disturbance. In total, six BBB types were recorded in this study, namely: bud-bearing root, rhizome, rhizophore, woody rhizome, stem tuber, and corms. The bud-bearing root from woody species was the dominant BBB in this system. Main conclusion The return of species that occurs in the first year after fire occurs rapidly in the first 2 months as resprouting species (primarily OS) appear in the system. Following this, the rate of return of species decreases as reseeding species (primarily NS) gradually reestablish and occupy the gaps in the dune fynbos-thicket vegetation. This study has shown that plant species in this ecosystem are adapted to fire disturbance as their populations are able to reestablish through various regeneration strategies, including resprouting from different types of BBB. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Gcina, Sinenjongo
- Date: 2022-12
- Subjects: Fynbos ecology – Nelson Mandela University Campus Nature Reserve , Forest fires -- Prevention and control
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59666 , vital:62319
- Description: Aim This study aims to provide an understanding and a description of the short-term postfire regeneration and ecology of the fynbos-thicket vegetation on a coastal dune system in the southeastern Cape Floristic Region. Another aim is to assess the recovery of the vegetation community by assessing the growth forms and the disturbance response across a range of species from thicket and fynbos communities and to provide a belowground bud bank classification for each of these species in the coastal dune systems. Location The study site was the Mandela University Reserve and the Noordhoek dune fields, situated in Gqeberha, Eastern Cape, which is part of the south-eastern Cape Floristic Region. The study site hosts thicket-fynbos mosaic vegetation type that occurs in a Mediterranean-Climate Ecosystem (MCE). Methods Firstly, field observations and photographs of plant species were taken weekly to assess and identify the species that were emerging and persisting in the dune system in the first 12 months postfire. Phenological events of the species that emerged (e.g., flowering, fruiting, leaf growth, leaf yellowing, and leaf abscission) were also observed and reported on. Secondly, an excavation of 21 species (e.g., Jamesbrittenia microphylla, Euclea racemosa, Olea exasperata, Searsia laevigata) was conducted to classify the species into their belowground bud bank types. Results There was a rapid emergence of resprouters (facultative resprouters and obligate resprouters) in the first 12 months postfire. This was followed by their growth; primarily occurring after the rainfall events. The dominant resprouting species (facultative resprouters and obligate resprouters) entered the system two weeks after the fire. There was a significant difference in the time to the first emergence of the different regeneration groups postfire (P < 0.001): on average, obligate sprouters (OS) appeared 1 month after fire – two months earlier than facultative sprouters (FS) and four months earlier than non-sprouters (NS). There was no statistical evidence to suggest that the FS and non-sprouters NS emerged at different times postfire; however, most NS species were observed in the system 5 months after fire. 4 Exceptions were the NS species Pelargonium grossularoides and Mesembryanthemum aitonis, both annuals that emerged 1 month after fire. A wide range of OS species that appeared earlier in the system (e.g., Euclea racemosa, Olea exasperata, Searsia laevigata, and Lauridia tetragona) exhibit underground lateral growth with belowground bud banks (BBBs) from which they recover after disturbance. In total, six BBB types were recorded in this study, namely: bud-bearing root, rhizome, rhizophore, woody rhizome, stem tuber, and corms. The bud-bearing root from woody species was the dominant BBB in this system. Main conclusion The return of species that occurs in the first year after fire occurs rapidly in the first 2 months as resprouting species (primarily OS) appear in the system. Following this, the rate of return of species decreases as reseeding species (primarily NS) gradually reestablish and occupy the gaps in the dune fynbos-thicket vegetation. This study has shown that plant species in this ecosystem are adapted to fire disturbance as their populations are able to reestablish through various regeneration strategies, including resprouting from different types of BBB. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Caring behaviour of midwives who nurse woman during childbirth in Botswana
- Authors: Potokwane, Nancy Osupile
- Date: 2022-12
- Subjects: Midwives , Childbirth, Botswana
- Language: English
- Type: Master's theses , Thesis
- Identifier: http://hdl.handle.net/10948/59821 , vital:62437
- Description: Caring behaviour, which is defined as nurturing another to whom one feels a personal sense of commitment and responsibility, is fundamental to the psychosocial component of midwifery. Midwives ought to show respect, kindness and continuous psychosocial support during childbirth to women, who have the right to receive the highest possible standard of care and to be treated with dignity and respect before, during and after childbirth. However, an increasing number of complaints of the uncaring behaviour of midwives towards labouring women has indicated a problem that has not been fully researched. Therefore, the study aimed to identify and describe caring behaviours in the context of midwives in Botswana; determine the inhibiting factors of caring behaviours of midwives during childbirth; and make recommendations to midwives and unit managers based on Swanson’s (1991) Theory of Caring, which might lead to the optimisation of caring behaviour in Botswana midwives. A quantitative, descriptive research design was followed to answer the research questions, and a structured, self-administered questionnaire, which had been developed by the researcher based on the literature review, was used to capture data. A purposive sampling method was used to select 200 respondents working in maternity units in the Kgatleng and Greater Gaborone regional health districts in Botswana. The questionnaire was evaluated for reliability and validity to ensure that it consistently and accurately measured what it was intended to measure. A pilot study was conducted with 10 midwives who met the criteria for participation in the study to pre-test the feasibility of the questionnaire in preparation for the main study. In addition, ethical principles, such as informed consent, respect for persons, anonymity, confidentiality and beneficence were followed throughout the study. The questionnaire consisted of questions and statements. It was divided into three sections. Section A: Demographic attributes; Section B: Identification and description of the caring behaviour of midwives: Section C: Determination of the inhibiting factors of caring behaviours in midwifery. The data provided by the respondent’s answers to the questionnaires were statistically analysed using the Statistical Package for Social Science (SPSS®): Version 27 with the assistance of a statistician, thereby generating descriptive and inferential statistics. v The results of the data analysis revealed that most of the respondents provided positive responses, thereby indicating caring behaviour in midwifery practice. However, several factors that inhibit the caring behaviour of midwives were identified, such as a shortage of staff, work overload, a poor working environment, the inconsistent selection of staff for professional development, a lack of equipment, the young age of some of the woman (less than 15 years), the parity of the woman (more than one child) and the negative behaviour of many of the women. Therefore, recommendations for midwives and unit managers based on the five processes of Swanson’s (1991) Theory of Caring were made that could be used to optimise midwifery care in Botswana. , Thesis (MNurs)-- Faculty of Faculty of Health Sciences, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Potokwane, Nancy Osupile
- Date: 2022-12
- Subjects: Midwives , Childbirth, Botswana
- Language: English
- Type: Master's theses , Thesis
- Identifier: http://hdl.handle.net/10948/59821 , vital:62437
- Description: Caring behaviour, which is defined as nurturing another to whom one feels a personal sense of commitment and responsibility, is fundamental to the psychosocial component of midwifery. Midwives ought to show respect, kindness and continuous psychosocial support during childbirth to women, who have the right to receive the highest possible standard of care and to be treated with dignity and respect before, during and after childbirth. However, an increasing number of complaints of the uncaring behaviour of midwives towards labouring women has indicated a problem that has not been fully researched. Therefore, the study aimed to identify and describe caring behaviours in the context of midwives in Botswana; determine the inhibiting factors of caring behaviours of midwives during childbirth; and make recommendations to midwives and unit managers based on Swanson’s (1991) Theory of Caring, which might lead to the optimisation of caring behaviour in Botswana midwives. A quantitative, descriptive research design was followed to answer the research questions, and a structured, self-administered questionnaire, which had been developed by the researcher based on the literature review, was used to capture data. A purposive sampling method was used to select 200 respondents working in maternity units in the Kgatleng and Greater Gaborone regional health districts in Botswana. The questionnaire was evaluated for reliability and validity to ensure that it consistently and accurately measured what it was intended to measure. A pilot study was conducted with 10 midwives who met the criteria for participation in the study to pre-test the feasibility of the questionnaire in preparation for the main study. In addition, ethical principles, such as informed consent, respect for persons, anonymity, confidentiality and beneficence were followed throughout the study. The questionnaire consisted of questions and statements. It was divided into three sections. Section A: Demographic attributes; Section B: Identification and description of the caring behaviour of midwives: Section C: Determination of the inhibiting factors of caring behaviours in midwifery. The data provided by the respondent’s answers to the questionnaires were statistically analysed using the Statistical Package for Social Science (SPSS®): Version 27 with the assistance of a statistician, thereby generating descriptive and inferential statistics. v The results of the data analysis revealed that most of the respondents provided positive responses, thereby indicating caring behaviour in midwifery practice. However, several factors that inhibit the caring behaviour of midwives were identified, such as a shortage of staff, work overload, a poor working environment, the inconsistent selection of staff for professional development, a lack of equipment, the young age of some of the woman (less than 15 years), the parity of the woman (more than one child) and the negative behaviour of many of the women. Therefore, recommendations for midwives and unit managers based on the five processes of Swanson’s (1991) Theory of Caring were made that could be used to optimise midwifery care in Botswana. , Thesis (MNurs)-- Faculty of Faculty of Health Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Characterising termite mound spatial patterns in the Eastern Cape Karoo : applying drone remote sensing,GIS and spatial statistics
- Mngcele, Lizalise Sive Nqaba
- Authors: Mngcele, Lizalise Sive Nqaba
- Date: 2022-12
- Subjects: Drone aircraft in remote sensing – Eastern Cape Karoo , Termites , Geographic information Systems – South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59555 , vital:62169
- Description: The Eastern Cape Karoo in South Africa has been earmarked for potential Shale Gas development, which has necessitated the understanding of existing ecosystems to be quantiĄed pre-development, in order to have a baseline against which the exploration can be monitored. Termite mounds as baseline mechanisms, are known to be sensitive to ecosystem disturbance and because of their abundance in the exploration zone, have been studied as indicator species. They are both a surface and subsurface phenomena which makes them an ideal baseline monitoring mechanism. Termite mound height, basal circumference and geospatial data was collected against natural and anthropogenic factors: elevation, vegetation, water, soil, geology, human settlements and roads. Mound distributions were observed across four study sites, and seven plots, using a DJI Phantom 4 Pro drone, an aerial and ground survey. Observed mound data on the drone and aerial survey was compared to that of the ground survey. Overall, the drone survey outperformed the aerial survey in recording accurate termite mound data. This was largely attributed to the scale of the study which gave the drone a competitive advantage. It allowed for drone data to be collected at 40 m altitude with an image resolution of 2-6 cm/pixel on each plot. In addition, drone detection accuracy was improved through the ability to generate digital surface models (DSMs) through point clouds and overlaying them with orthomosaics. Considering observed mound spatial point patterns, both the drone and aerial survey were more than 50% percent consistent with the ground survey, although the drone survey detected 28.57% more accurate mound spatial point patterns than the aerial survey. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Mngcele, Lizalise Sive Nqaba
- Date: 2022-12
- Subjects: Drone aircraft in remote sensing – Eastern Cape Karoo , Termites , Geographic information Systems – South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59555 , vital:62169
- Description: The Eastern Cape Karoo in South Africa has been earmarked for potential Shale Gas development, which has necessitated the understanding of existing ecosystems to be quantiĄed pre-development, in order to have a baseline against which the exploration can be monitored. Termite mounds as baseline mechanisms, are known to be sensitive to ecosystem disturbance and because of their abundance in the exploration zone, have been studied as indicator species. They are both a surface and subsurface phenomena which makes them an ideal baseline monitoring mechanism. Termite mound height, basal circumference and geospatial data was collected against natural and anthropogenic factors: elevation, vegetation, water, soil, geology, human settlements and roads. Mound distributions were observed across four study sites, and seven plots, using a DJI Phantom 4 Pro drone, an aerial and ground survey. Observed mound data on the drone and aerial survey was compared to that of the ground survey. Overall, the drone survey outperformed the aerial survey in recording accurate termite mound data. This was largely attributed to the scale of the study which gave the drone a competitive advantage. It allowed for drone data to be collected at 40 m altitude with an image resolution of 2-6 cm/pixel on each plot. In addition, drone detection accuracy was improved through the ability to generate digital surface models (DSMs) through point clouds and overlaying them with orthomosaics. Considering observed mound spatial point patterns, both the drone and aerial survey were more than 50% percent consistent with the ground survey, although the drone survey detected 28.57% more accurate mound spatial point patterns than the aerial survey. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Converged data and sensing over optical fiber networks
- Authors: Shumane, Vela
- Date: 2022-12
- Subjects: Optical fiber subscriber loops -- South Africa , Chemical detectors , Internet -- Technological innovations
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59968 , vital:62722
- Description: Internet connectivity, data and sensors have become increasingly important across all spheres of business and industry, especially in the mining sector. Recent years have seen deeper mining explorations as a result of the depletion of natural resources in shallow strata. Due to complex and unexpected geological conditions as well as significant ground stresses, deep stratum mining operations encounter a number of difficulties. It is essential that the mining industry be more innovative with their equipment and monitoring systems given the rise in expenses caused by energy consumption, concessions to surface integrity, worldwide freshwater shortage, as well as health and safety of miners. Any attempt to eliminate these mining consequences must start with early discovery. An effective plan to anticipate, prevent, or manage geohazards events must be in place because to these complex and unpredictably occurring geological circumstances. Due to their capacity to combine gigabits of data from remote locations within the mine to a centralized control centre, optical fiber offers a variety of distinctive advantages within the mining industry. In order to attain maximum productivity, modern and effective mining operations use enhanced control techniques and increasing mechanization. Additionally, optical fibers can be utilized in a mine to safely monitor seismic activity, methane, roof collapses, rock bursts, explosions, and dangerous underground mine settings. Multimode or multi-core fibers represent a particularly intriguing alternative for transmissions over small distances, especially for broad band local area networks like LANs, as they enable the use of affordable components. Due to the current state of these issues, there is a drive to create fiber optic communication links that can also function as distributed optical fiber sensors, where each point along the fiber can function as a continuous array of sensors. In this thesis, we suggested and experimentally demonstrated a converged solution for precise vibration sensing and high-speed data in mining applications. With wireless access for people and equipment inside cavities, the solution uses multimode fiber to link nearby mining cavities. To track vibrations and earth tremors causing rock falls, polarization-based vibration sensors over multimode fiber is used. With a modal dispersion penalty of just 1.6 dB, photonic data transmission across 100 m of multimode fiber is successfully accomplished. Successful 1.7 GHz wireless transmission across a distance of 1 m is demonstrated, and vibrations between 50 Hz and 1 kHz may be reliably detected to within 0.02 percent of the true value. , Thesis (MSc) -- Faculty of Science, School of Computer Science, Mathematics, Physics and Statistics, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Shumane, Vela
- Date: 2022-12
- Subjects: Optical fiber subscriber loops -- South Africa , Chemical detectors , Internet -- Technological innovations
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59968 , vital:62722
- Description: Internet connectivity, data and sensors have become increasingly important across all spheres of business and industry, especially in the mining sector. Recent years have seen deeper mining explorations as a result of the depletion of natural resources in shallow strata. Due to complex and unexpected geological conditions as well as significant ground stresses, deep stratum mining operations encounter a number of difficulties. It is essential that the mining industry be more innovative with their equipment and monitoring systems given the rise in expenses caused by energy consumption, concessions to surface integrity, worldwide freshwater shortage, as well as health and safety of miners. Any attempt to eliminate these mining consequences must start with early discovery. An effective plan to anticipate, prevent, or manage geohazards events must be in place because to these complex and unpredictably occurring geological circumstances. Due to their capacity to combine gigabits of data from remote locations within the mine to a centralized control centre, optical fiber offers a variety of distinctive advantages within the mining industry. In order to attain maximum productivity, modern and effective mining operations use enhanced control techniques and increasing mechanization. Additionally, optical fibers can be utilized in a mine to safely monitor seismic activity, methane, roof collapses, rock bursts, explosions, and dangerous underground mine settings. Multimode or multi-core fibers represent a particularly intriguing alternative for transmissions over small distances, especially for broad band local area networks like LANs, as they enable the use of affordable components. Due to the current state of these issues, there is a drive to create fiber optic communication links that can also function as distributed optical fiber sensors, where each point along the fiber can function as a continuous array of sensors. In this thesis, we suggested and experimentally demonstrated a converged solution for precise vibration sensing and high-speed data in mining applications. With wireless access for people and equipment inside cavities, the solution uses multimode fiber to link nearby mining cavities. To track vibrations and earth tremors causing rock falls, polarization-based vibration sensors over multimode fiber is used. With a modal dispersion penalty of just 1.6 dB, photonic data transmission across 100 m of multimode fiber is successfully accomplished. Successful 1.7 GHz wireless transmission across a distance of 1 m is demonstrated, and vibrations between 50 Hz and 1 kHz may be reliably detected to within 0.02 percent of the true value. , Thesis (MSc) -- Faculty of Science, School of Computer Science, Mathematics, Physics and Statistics, 2022
- Full Text:
- Date Issued: 2022-12
Cost control challenges in Eastern Cape Department of Health
- Authors: Ntete, Patrick
- Date: 2022-12
- Subjects: Cost control, Eastern Cape , Health Department
- Language: English
- Type: Master's theses , Thesis
- Identifier: http://hdl.handle.net/10948/59799 , vital:62435
- Description: Cost control in the South African public health sector has become a major concern due to the amount of money that the government loses every financial year. If costs in the South African public health sector are not kept under control there might be catastrophic consequences. Regardless of the challenges in controlling costs in the health sector, scant studies have explored the cost control phenomenon, which is seen as a scientific management technique to control and lessen the cost of conducting business. Keeping costs under control in the health sector became particularly important during the COVID-19 pandemic when the budgetary burden on the health sector worsened. This study aimed to identify challenges to cost control in the Eastern Cape Department of Health and come up with recommendations on how the challenges can be addressed. The study followed a qualitative research approach and an interview schedule with open-ended questions was prepared to answer the research questions that were formulated. The research sample consisted of 13 employees of the Eastern Cape Department of Health with work experience ranging between 11 and 15 years to ensure credible data. The study’s findings revealed that the participants who were interviewed had an idea of the cost control concept; that cost containment committees help reduce costs by monitoring and evaluating expenditure to ensure that the funds that are spent result in added value; that cost control is an important concept in the health sector, as it ensures that expenditure is reduced and that the influx of undocumented immigrants exerts unexpected pressure on the health sector as these people are generally not taken into account when the budget is drawn up. Findings from the study could assist in formulating strategies that can be employed to improve cost control in the health sector, for example, immunisation and educating employees and management about cost control measures and how they can be implemented. , Thesis (MBA) -- Faculty of Business and Economic Science, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Ntete, Patrick
- Date: 2022-12
- Subjects: Cost control, Eastern Cape , Health Department
- Language: English
- Type: Master's theses , Thesis
- Identifier: http://hdl.handle.net/10948/59799 , vital:62435
- Description: Cost control in the South African public health sector has become a major concern due to the amount of money that the government loses every financial year. If costs in the South African public health sector are not kept under control there might be catastrophic consequences. Regardless of the challenges in controlling costs in the health sector, scant studies have explored the cost control phenomenon, which is seen as a scientific management technique to control and lessen the cost of conducting business. Keeping costs under control in the health sector became particularly important during the COVID-19 pandemic when the budgetary burden on the health sector worsened. This study aimed to identify challenges to cost control in the Eastern Cape Department of Health and come up with recommendations on how the challenges can be addressed. The study followed a qualitative research approach and an interview schedule with open-ended questions was prepared to answer the research questions that were formulated. The research sample consisted of 13 employees of the Eastern Cape Department of Health with work experience ranging between 11 and 15 years to ensure credible data. The study’s findings revealed that the participants who were interviewed had an idea of the cost control concept; that cost containment committees help reduce costs by monitoring and evaluating expenditure to ensure that the funds that are spent result in added value; that cost control is an important concept in the health sector, as it ensures that expenditure is reduced and that the influx of undocumented immigrants exerts unexpected pressure on the health sector as these people are generally not taken into account when the budget is drawn up. Findings from the study could assist in formulating strategies that can be employed to improve cost control in the health sector, for example, immunisation and educating employees and management about cost control measures and how they can be implemented. , Thesis (MBA) -- Faculty of Business and Economic Science, 2022
- Full Text:
- Date Issued: 2022-12
Development of a continuous flow process towards the synthesis of fluvastatin: a lipid lowering drug
- Authors: Singata, Mzuvukile
- Date: 2022-12
- Subjects: Drug development – South Africa , Drugs --Law and legislation , Pharmaceutical technology -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59587 , vital:62175
- Description: Access to pharmaceuticals in low to middle income countries affects not only the health of a nation, but also the economy. Global pandemics such as the SARS COVID-19 pandemic have demonstrated this disparity. A potential solution to this crisis is an efficient and reliable means of local production of active pharmaceutical ingredients. Current manufacturing plants heavily rely on batch processes as their primary form of production. Batch production has been shown to have numerous disadvantages that impede the reliable production of drugs. Flow chemistry presents a possible solution to this impediment. Numerous advantages of flow chemistry make this technology a better and safer option for production; these include improved safety, faster reaction times and improved reaction control. In this research we present the use of flow chemistry towards the synthesis of fluvastatin, a lipid lowering drug for individuals that suffer from hypercholesterolemia, which is characterised by elevated levels of low-density lipoprotein that is sometimes referred to as bad cholesterol. Chapter 1 provides a detailed description of the background to the problem of hypercholesterolemia; in this chapter the role of cholesterol in the body and how it can end up becoming a problem is outlined. An in-depth analysis of flow chemistry is provided and finally the problem statement, aims and objectives are presented. Chapter 2 give a detailed description of the instrumentation and chemicals used in the research. A description of experimental methodology towards the synthesis of all intermediates in batch and flow systems is provided. Chapter 3 provides all the data collected from the research and describes a multivariate optimization of the intermediates towards the synthesis of fluvastatin. The first intermediate was successfully synthesized in batch (60%) and flow systems at an improved conversion of 98% in a residence time of 15 minutes. The second intermediate was also obtained in batch (32%) and using continuous flow systems at a conversion of 75% and a residence time of 25 minutes. The third intermediate was successfully cyclized in batch using ZnCl2 (50%) and via continuous flow using Amberlite IR120 in a packed column reactor at a residence time of 15 minutes at 95°C, to obtain a conversion of 98%. The final intermediate was successfully synthesized in batch (62%) and in flow; it was obtained at 98% at a residence time of 15 minutes using continuous flow. Chapter 4 provides a conclusion of the study; it also provides a description of the future work and recommendations for this research. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Development of a continuous flow process towards the synthesis of fluvastatin: a lipid lowering drug
- Authors: Singata, Mzuvukile
- Date: 2022-12
- Subjects: Drug development – South Africa , Drugs --Law and legislation , Pharmaceutical technology -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59587 , vital:62175
- Description: Access to pharmaceuticals in low to middle income countries affects not only the health of a nation, but also the economy. Global pandemics such as the SARS COVID-19 pandemic have demonstrated this disparity. A potential solution to this crisis is an efficient and reliable means of local production of active pharmaceutical ingredients. Current manufacturing plants heavily rely on batch processes as their primary form of production. Batch production has been shown to have numerous disadvantages that impede the reliable production of drugs. Flow chemistry presents a possible solution to this impediment. Numerous advantages of flow chemistry make this technology a better and safer option for production; these include improved safety, faster reaction times and improved reaction control. In this research we present the use of flow chemistry towards the synthesis of fluvastatin, a lipid lowering drug for individuals that suffer from hypercholesterolemia, which is characterised by elevated levels of low-density lipoprotein that is sometimes referred to as bad cholesterol. Chapter 1 provides a detailed description of the background to the problem of hypercholesterolemia; in this chapter the role of cholesterol in the body and how it can end up becoming a problem is outlined. An in-depth analysis of flow chemistry is provided and finally the problem statement, aims and objectives are presented. Chapter 2 give a detailed description of the instrumentation and chemicals used in the research. A description of experimental methodology towards the synthesis of all intermediates in batch and flow systems is provided. Chapter 3 provides all the data collected from the research and describes a multivariate optimization of the intermediates towards the synthesis of fluvastatin. The first intermediate was successfully synthesized in batch (60%) and flow systems at an improved conversion of 98% in a residence time of 15 minutes. The second intermediate was also obtained in batch (32%) and using continuous flow systems at a conversion of 75% and a residence time of 25 minutes. The third intermediate was successfully cyclized in batch using ZnCl2 (50%) and via continuous flow using Amberlite IR120 in a packed column reactor at a residence time of 15 minutes at 95°C, to obtain a conversion of 98%. The final intermediate was successfully synthesized in batch (62%) and in flow; it was obtained at 98% at a residence time of 15 minutes using continuous flow. Chapter 4 provides a conclusion of the study; it also provides a description of the future work and recommendations for this research. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Development of a crumb rubber adsorbent for the sorption of lead and copper from waste water: Optimization of sorption in a binary component system
- Authors: Hlabana,Relebohile Gladys
- Date: 2022-12
- Subjects: Port Elizabeth (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59688 , vital:62356
- Description: Waste tyre is becoming a global environmental problem due to challenges in its disposal management and the fact that it poses fire hazards and metal leaching risk. Waste tyre can also breed mosquitos if it traps water thereby becoming an environmental health problem. There is also a portion of unused tyre in a waste tyre that is valuable and should be recycled. In South Africa waste tyre recycling is in three industries, one of which is crumb rubber where waste tyre is applied into various sized particles called crumb rubber. Crumb rubber can be applied in environmental remediation as an organic and inorganic pollutants’ adsorbent. Considering the fact that South Africa’s environment is loaded with heavy metal pollutants due to contributions by mining and agriculture as examples, it is postulated that crumb rubber produced in South Africa could be used to remediate the environment of these toxic metals. In this study adsorption of Pb (II) and Cu (II) from the synthetic waste water using waste tyre crumb rubber produced in South Africa was studied. Crumb rubber was activated using aqueous NaOH (0.02%) to make crumb rubber wettable followed by it being treated with 0.02 % acidified KMnO4 for modification of its ability to sorp positively charged metal species. Trial sorption experiments on 50 mg / L Cu (II) were successful with sorption of over 70% in 250 minutes. Final optimization of the sorption of combined Pb (II) and Cu (II) was studied using a full factorial design and optimum conditions of sorption were found to be pH 5.4; [metal] 0.617 mmol / L for Pb (II); and pH 5.4; the optimization of Cu (II) was found to be pH 5.4; [metal] 0.777 mmol / L. In Pb (II) sorptions the mechanism of adsorption on the activated crumb rubber was found to be following the Freundlich model indicating that the adsorption seemed to occur on a multilayer adsorbent, while the mechanism of copper(II) adsorption on to the activated crumb rubber was found to be following Langmuir model, indicating that the adsorption seemed to occur on a monolayer. With adsorption percentages ranging from 98.2 % to 100.0 %, for Pb (II), the crumb rubber produced under the conditions of this study, can be used in South Africa for selective removal of Pb (II). , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Hlabana,Relebohile Gladys
- Date: 2022-12
- Subjects: Port Elizabeth (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59688 , vital:62356
- Description: Waste tyre is becoming a global environmental problem due to challenges in its disposal management and the fact that it poses fire hazards and metal leaching risk. Waste tyre can also breed mosquitos if it traps water thereby becoming an environmental health problem. There is also a portion of unused tyre in a waste tyre that is valuable and should be recycled. In South Africa waste tyre recycling is in three industries, one of which is crumb rubber where waste tyre is applied into various sized particles called crumb rubber. Crumb rubber can be applied in environmental remediation as an organic and inorganic pollutants’ adsorbent. Considering the fact that South Africa’s environment is loaded with heavy metal pollutants due to contributions by mining and agriculture as examples, it is postulated that crumb rubber produced in South Africa could be used to remediate the environment of these toxic metals. In this study adsorption of Pb (II) and Cu (II) from the synthetic waste water using waste tyre crumb rubber produced in South Africa was studied. Crumb rubber was activated using aqueous NaOH (0.02%) to make crumb rubber wettable followed by it being treated with 0.02 % acidified KMnO4 for modification of its ability to sorp positively charged metal species. Trial sorption experiments on 50 mg / L Cu (II) were successful with sorption of over 70% in 250 minutes. Final optimization of the sorption of combined Pb (II) and Cu (II) was studied using a full factorial design and optimum conditions of sorption were found to be pH 5.4; [metal] 0.617 mmol / L for Pb (II); and pH 5.4; the optimization of Cu (II) was found to be pH 5.4; [metal] 0.777 mmol / L. In Pb (II) sorptions the mechanism of adsorption on the activated crumb rubber was found to be following the Freundlich model indicating that the adsorption seemed to occur on a multilayer adsorbent, while the mechanism of copper(II) adsorption on to the activated crumb rubber was found to be following Langmuir model, indicating that the adsorption seemed to occur on a monolayer. With adsorption percentages ranging from 98.2 % to 100.0 %, for Pb (II), the crumb rubber produced under the conditions of this study, can be used in South Africa for selective removal of Pb (II). , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Different dimensions of inequality and sustainable economic growth in Africa
- Authors: Raimi, Rasaq Being
- Date: 2022-12
- Subjects: Inequality , Sustainable development , Economic growth -- Africa
- Language: English
- Type: Doctoral's theses , Thesis
- Identifier: http://hdl.handle.net/10948/59884 , vital:62483
- Description: The recent unprecedented economic growth across Africa for over two decades has been anticipated to alleviate poverty and close up the wide inequality gap in the continent; its impact, however, on the vast majority of people in Africa appears to be insignificant as more households are still battling with poverty and the inequality gap is still wide. This has, however, raised empirical questions regarding the nature of the relationship that exists between economic growth and inequality in Africa. Hence, these questions have created gaps in the literature as some studies found positive relationship while other results state otherwise. The study fills the existing gap by re-examining the relationship between dimensions of inequality and sustainable growth in Africa. The study applied parametric and non-parametric econometric techniques on more recent inequalities data covering 28 African countries. Specifically, the study examined the relationship between income inequality and sustainable growth in Africa, investigated the relationship between education inequality and sustainable growth in Africa, analysed the relationship between gender inequality and sustainable growth in Africa and discussed the relationship between land inequality and sustainable growth in Africa. The study covered the period of 1998-2020 and the data for the study were sourced from Standardised World Income Inequality Data Base (SWIID), World Economic Forum, World Bank Online Data Base, Benaabdelaali et al‘s (2012) paper and Ziesemer, THW‘s (2016) paper. Result from the analysis of income inequality and sustainable growth indicates a positive unidirectional relationship between the two variables. The findings suggest that African growth promotes more income inequality and income inequality does not interrupt the growth potential of the continent. The findings further reveal a positive relationship between sustainable growth and poverty rate in African region. This suggests that African economic growth alone cannot solve African poverty problem. Analysis of sustainable growth and education inequality shows an inverse relationship between the two variables. The result indicates that sustainable growth helps in reducing education inequality but a further increase in education inequality drags down sustainable growth in the long-run. Also, poverty is found to have a positive relationship with education inequality in Africa. However, no causal relationship is found between sustainable growth and education inequality but rather a unidirectional causality running from poverty to education inequality in Africa. iv In relation to the gender inequality analysis, the study also found a negative relationship between the two variables. Specifically, while sustainable growth is found to be bridging gender gap, gender inequality on the other hand is found to be a limiting factor on sustainable growth in Africa. The results also indicate a strong positive significant relationship between poverty and gender inequality in Africa. The analysis reveals the vulnerability of African women to poverty and also suggests that many African countries are yet to harness the enormous potentials possessed by women for their regional development. Furthermore, the study found a negative relationship between sustainable growth and land inequality in Africa. The result indicates that unequal land distribution is among the factors preventing Africa from achieving its high dream of sustainable growth and it is further responsible for the severe poverty especially among the rural dwellers where agricultural land is considered as an important determinant of family income. One way causality running from land inequality to sustainable growth is also established via granger causality result which further indicates that uneven land distribution has effects on sustainable growth. Based on these findings, the study concludes that economic growth though has been helping in bridging some dimensions of inequality in Africa, income related inequality is exempted. Also, it is evident that economic growth alone is not adequate to close the wide inequality gap that has persisted for so long in Africa. In conclusion, the study recommended among other things that many African policy makers need to intensify their developmental efforts to reach everyone in their countries so as to effectively fight the ugly poverty situation across the continent. Also, salary structure needs to be revisited across Africa via introduction of appropriate tax to redistribute income and help the low income earners to relatively meet up with the higher income earners. This will help in closing income gap and at the same time alleviate poverty. , Thesis (PHD) -- Faculty of Business and Economic Science, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Raimi, Rasaq Being
- Date: 2022-12
- Subjects: Inequality , Sustainable development , Economic growth -- Africa
- Language: English
- Type: Doctoral's theses , Thesis
- Identifier: http://hdl.handle.net/10948/59884 , vital:62483
- Description: The recent unprecedented economic growth across Africa for over two decades has been anticipated to alleviate poverty and close up the wide inequality gap in the continent; its impact, however, on the vast majority of people in Africa appears to be insignificant as more households are still battling with poverty and the inequality gap is still wide. This has, however, raised empirical questions regarding the nature of the relationship that exists between economic growth and inequality in Africa. Hence, these questions have created gaps in the literature as some studies found positive relationship while other results state otherwise. The study fills the existing gap by re-examining the relationship between dimensions of inequality and sustainable growth in Africa. The study applied parametric and non-parametric econometric techniques on more recent inequalities data covering 28 African countries. Specifically, the study examined the relationship between income inequality and sustainable growth in Africa, investigated the relationship between education inequality and sustainable growth in Africa, analysed the relationship between gender inequality and sustainable growth in Africa and discussed the relationship between land inequality and sustainable growth in Africa. The study covered the period of 1998-2020 and the data for the study were sourced from Standardised World Income Inequality Data Base (SWIID), World Economic Forum, World Bank Online Data Base, Benaabdelaali et al‘s (2012) paper and Ziesemer, THW‘s (2016) paper. Result from the analysis of income inequality and sustainable growth indicates a positive unidirectional relationship between the two variables. The findings suggest that African growth promotes more income inequality and income inequality does not interrupt the growth potential of the continent. The findings further reveal a positive relationship between sustainable growth and poverty rate in African region. This suggests that African economic growth alone cannot solve African poverty problem. Analysis of sustainable growth and education inequality shows an inverse relationship between the two variables. The result indicates that sustainable growth helps in reducing education inequality but a further increase in education inequality drags down sustainable growth in the long-run. Also, poverty is found to have a positive relationship with education inequality in Africa. However, no causal relationship is found between sustainable growth and education inequality but rather a unidirectional causality running from poverty to education inequality in Africa. iv In relation to the gender inequality analysis, the study also found a negative relationship between the two variables. Specifically, while sustainable growth is found to be bridging gender gap, gender inequality on the other hand is found to be a limiting factor on sustainable growth in Africa. The results also indicate a strong positive significant relationship between poverty and gender inequality in Africa. The analysis reveals the vulnerability of African women to poverty and also suggests that many African countries are yet to harness the enormous potentials possessed by women for their regional development. Furthermore, the study found a negative relationship between sustainable growth and land inequality in Africa. The result indicates that unequal land distribution is among the factors preventing Africa from achieving its high dream of sustainable growth and it is further responsible for the severe poverty especially among the rural dwellers where agricultural land is considered as an important determinant of family income. One way causality running from land inequality to sustainable growth is also established via granger causality result which further indicates that uneven land distribution has effects on sustainable growth. Based on these findings, the study concludes that economic growth though has been helping in bridging some dimensions of inequality in Africa, income related inequality is exempted. Also, it is evident that economic growth alone is not adequate to close the wide inequality gap that has persisted for so long in Africa. In conclusion, the study recommended among other things that many African policy makers need to intensify their developmental efforts to reach everyone in their countries so as to effectively fight the ugly poverty situation across the continent. Also, salary structure needs to be revisited across Africa via introduction of appropriate tax to redistribute income and help the low income earners to relatively meet up with the higher income earners. This will help in closing income gap and at the same time alleviate poverty. , Thesis (PHD) -- Faculty of Business and Economic Science, 2022
- Full Text:
- Date Issued: 2022-12
Economic impact assessment of the national youth development agency (NYDA) grant fund in the Eastern Cape
- Authors: Fotoyi, Asanda
- Date: 2022-12
- Subjects: Economic impact analysis , Youth development, Eastern Cape
- Language: English
- Type: Doctoral's theses , Thesis
- Identifier: http://hdl.handle.net/10948/59424 , vital:62093
- Description: Since the dawn of democracy in South Africa, the country has adopted several youth policies aimed at promoting young peoples’ education and training, employment, entrepreneurship, and healthy lifestyles. Still, however, the country battles high youth unemployment rates, which increased from 35.7% in 2010 to 41.0% in 2019. Such issues exist despite the NYDA being established in 2008 to coordinate youth strategies in the country. The NYDA strategic areas include: economic participation, education and skills development, health and wellbeing, knowledge management, and governance. Of note is that NYDA grant funding has been specifically designed to provide young South African entrepreneurs with an opportunity to access both financial and nonfinancial business support to establish or expand their businesses. Over a period of 7 years (2013-2019), the NYDA has grant funded a significant number of youth-owned businesses across the country, to the value of about R204.5 million. Given the large amount of public funds invested through NYDA grant funding, it is important to assess whether or not this investment has been favourable in terms of achieving its goals. This determination is especially important, as governmental resources are scarce and subject to opportunity cost, in that some alternatives have been forgone in favour of the NYDA mandate. The present study was, therefore, necessary, as, since the inception of NYDA grant funding, its economic impact has not been scientifically measured or assessed. As such, there has not been any scientific basis to justify NYDA grant funding. In particular, this thesis assessed the economic impact of the NYDA grant funding in the Eastern Cape. The effectiveness of the NYDA grant funding was examined against its objectives: improved entrepreneurship participation, business skills development and support, and improved creation of job opportunities. The primary hypothesis was tested using a cost-benefit analysis (CBA). The CBA method was adopted as it is a well-established economic assessment method to test policy interventions. This method is also based on methodological rigour and was determined to be most applicable for this present research endeavour. v The notion behind using the CBA was to quantify the net benefits of NYDA grant funding for youth businesses by comparing the benefits with the corresponding costs. In order to determine other factors that influence the turnovers of NYDA grant funding recipients’ businesses, secondary hypotheses were then tested by using a least absolute deviation (LAD) regression analysis in respect to cross-sectional data relating to the respondents’ annual turnovers over the years 2014-2019. The LAD estimation method was used primarily due to its robustness in comparison to ordinary least squares (OLS). This study made use of a questionnaire to collect data on the demographics of the NYDA grant funding beneficiaries. As well as the beneficiaries’ business characteristics, their subjective perceptions on the impact of the NYDA support interventions, and business-specific challenges. The Raosoft calculator was used to calculate the sample size at a 5% margin of error. The scientifically sound sample size of the respondents, according to the Raosoft calculator, was 246 out of 681 clients. The respondents were drawn from the Eastern Cape areas by means of face-to-face survey interviews as well as a digitised research instrument that was circulated using several online platforms (e.g., email, WhatsApp, and Facebook). The total number of respondents who ultimately participated in this study was 253. In order to achieve Denzin’s (1978) triangulation, a focus group session was also conducted with selected NYDA grant beneficiaries. The focus group discussion for this research was held using the online platform, Microsoft Teams (MS Teams). The survey analysis findings of the study suggest that the demographics of the NYDA grant funding beneficiaries are a general reflection of the Eastern Cape’s overall youth entrepreneurship trends. As such, there were more male youth entrepreneurs compared to female youth entrepreneurs. The involvement of youth in business improved in correspondence with age -groups. The population groups of the grant funding beneficiaries also reflected the country’s racial demographics. In terms of business characteristics, the survey analysis findings revealed that NYDA grant funded businesses function across various sectors of the economy and largely operate out of homes. These businesses primarily serve local households and communities, have generally been operating for 3-5 years, and were largely started because the owner recognised an opportunity in the market. In terms of business assistance from vi NYDA, it was found that the grant funding was mostly utilised for asset finance. In terms of business challenges, market conditions were highlighted as negatively impacting youth businesses. However, the participating business owners were primarily inward-looking when seeking to address their challenges. A further finding from the survey analysis revealed that many NYDA grant funded businesses tended to increase the number of jobs created in the period under review. The CBA findings, in turn, showed that the public expenditure in Eastern Cape youth businesses through NYDA grant funding was economically viable during the period under review, since the benefit-cost ratio (BCR) exceeded ‘1’ (the net present value (NPV) was also positive). The regression estimation findings further indicated gender, education, and industry gross domestic product (GDP) growth rates also had a significant impact on the annual turnovers of the youth businesses. The focus group findings highlighted that most beneficiaries derive value from NYDA grant funding. However, there is scope for improvement in the areas of access to finance, turnaround times, access to markets, links to the entrepreneurship ecosystem, administrative burden, mentorship, monitoring and evaluation (M&E), and organisational culture. Based on the findings of the study, this thesis concludes that in the period under review, in the Eastern Cape, NYDA grant funding yields positive social returns; and delivered on its objectives (i.e., improved entrepreneurship participation, business skills development and support, and improved creation of job opportunities). The empirical findings of this study further supports this thesis’s recommendation for: 1) improvements in the NYDA grant funding budget; 2) closing gender gaps in youth entrepreneurship; 3) improving participation of disabled youth in business support services; 4) promoting entrepreneurship as a career path; 5) providing youth business support in rural areas; 6) diversifying economic sectors of youth businesses; 7) offering access to finance for youth business growth and expansion; 8) establishing youth businesses access to markets; 9) reducing administrative burdens for youth businesses; 10) improving accessibility of business development trainings; 11) offering mentorship for youth entrepreneurs, 12) providing infrastructure for youth businesses; 13) extend Covid-19 relief support for youth businesses; and 14) standardising practises in youth business support services. , Thesis (PHD) -- Faculty of Business and Economics Science, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Fotoyi, Asanda
- Date: 2022-12
- Subjects: Economic impact analysis , Youth development, Eastern Cape
- Language: English
- Type: Doctoral's theses , Thesis
- Identifier: http://hdl.handle.net/10948/59424 , vital:62093
- Description: Since the dawn of democracy in South Africa, the country has adopted several youth policies aimed at promoting young peoples’ education and training, employment, entrepreneurship, and healthy lifestyles. Still, however, the country battles high youth unemployment rates, which increased from 35.7% in 2010 to 41.0% in 2019. Such issues exist despite the NYDA being established in 2008 to coordinate youth strategies in the country. The NYDA strategic areas include: economic participation, education and skills development, health and wellbeing, knowledge management, and governance. Of note is that NYDA grant funding has been specifically designed to provide young South African entrepreneurs with an opportunity to access both financial and nonfinancial business support to establish or expand their businesses. Over a period of 7 years (2013-2019), the NYDA has grant funded a significant number of youth-owned businesses across the country, to the value of about R204.5 million. Given the large amount of public funds invested through NYDA grant funding, it is important to assess whether or not this investment has been favourable in terms of achieving its goals. This determination is especially important, as governmental resources are scarce and subject to opportunity cost, in that some alternatives have been forgone in favour of the NYDA mandate. The present study was, therefore, necessary, as, since the inception of NYDA grant funding, its economic impact has not been scientifically measured or assessed. As such, there has not been any scientific basis to justify NYDA grant funding. In particular, this thesis assessed the economic impact of the NYDA grant funding in the Eastern Cape. The effectiveness of the NYDA grant funding was examined against its objectives: improved entrepreneurship participation, business skills development and support, and improved creation of job opportunities. The primary hypothesis was tested using a cost-benefit analysis (CBA). The CBA method was adopted as it is a well-established economic assessment method to test policy interventions. This method is also based on methodological rigour and was determined to be most applicable for this present research endeavour. v The notion behind using the CBA was to quantify the net benefits of NYDA grant funding for youth businesses by comparing the benefits with the corresponding costs. In order to determine other factors that influence the turnovers of NYDA grant funding recipients’ businesses, secondary hypotheses were then tested by using a least absolute deviation (LAD) regression analysis in respect to cross-sectional data relating to the respondents’ annual turnovers over the years 2014-2019. The LAD estimation method was used primarily due to its robustness in comparison to ordinary least squares (OLS). This study made use of a questionnaire to collect data on the demographics of the NYDA grant funding beneficiaries. As well as the beneficiaries’ business characteristics, their subjective perceptions on the impact of the NYDA support interventions, and business-specific challenges. The Raosoft calculator was used to calculate the sample size at a 5% margin of error. The scientifically sound sample size of the respondents, according to the Raosoft calculator, was 246 out of 681 clients. The respondents were drawn from the Eastern Cape areas by means of face-to-face survey interviews as well as a digitised research instrument that was circulated using several online platforms (e.g., email, WhatsApp, and Facebook). The total number of respondents who ultimately participated in this study was 253. In order to achieve Denzin’s (1978) triangulation, a focus group session was also conducted with selected NYDA grant beneficiaries. The focus group discussion for this research was held using the online platform, Microsoft Teams (MS Teams). The survey analysis findings of the study suggest that the demographics of the NYDA grant funding beneficiaries are a general reflection of the Eastern Cape’s overall youth entrepreneurship trends. As such, there were more male youth entrepreneurs compared to female youth entrepreneurs. The involvement of youth in business improved in correspondence with age -groups. The population groups of the grant funding beneficiaries also reflected the country’s racial demographics. In terms of business characteristics, the survey analysis findings revealed that NYDA grant funded businesses function across various sectors of the economy and largely operate out of homes. These businesses primarily serve local households and communities, have generally been operating for 3-5 years, and were largely started because the owner recognised an opportunity in the market. In terms of business assistance from vi NYDA, it was found that the grant funding was mostly utilised for asset finance. In terms of business challenges, market conditions were highlighted as negatively impacting youth businesses. However, the participating business owners were primarily inward-looking when seeking to address their challenges. A further finding from the survey analysis revealed that many NYDA grant funded businesses tended to increase the number of jobs created in the period under review. The CBA findings, in turn, showed that the public expenditure in Eastern Cape youth businesses through NYDA grant funding was economically viable during the period under review, since the benefit-cost ratio (BCR) exceeded ‘1’ (the net present value (NPV) was also positive). The regression estimation findings further indicated gender, education, and industry gross domestic product (GDP) growth rates also had a significant impact on the annual turnovers of the youth businesses. The focus group findings highlighted that most beneficiaries derive value from NYDA grant funding. However, there is scope for improvement in the areas of access to finance, turnaround times, access to markets, links to the entrepreneurship ecosystem, administrative burden, mentorship, monitoring and evaluation (M&E), and organisational culture. Based on the findings of the study, this thesis concludes that in the period under review, in the Eastern Cape, NYDA grant funding yields positive social returns; and delivered on its objectives (i.e., improved entrepreneurship participation, business skills development and support, and improved creation of job opportunities). The empirical findings of this study further supports this thesis’s recommendation for: 1) improvements in the NYDA grant funding budget; 2) closing gender gaps in youth entrepreneurship; 3) improving participation of disabled youth in business support services; 4) promoting entrepreneurship as a career path; 5) providing youth business support in rural areas; 6) diversifying economic sectors of youth businesses; 7) offering access to finance for youth business growth and expansion; 8) establishing youth businesses access to markets; 9) reducing administrative burdens for youth businesses; 10) improving accessibility of business development trainings; 11) offering mentorship for youth entrepreneurs, 12) providing infrastructure for youth businesses; 13) extend Covid-19 relief support for youth businesses; and 14) standardising practises in youth business support services. , Thesis (PHD) -- Faculty of Business and Economics Science, 2022
- Full Text:
- Date Issued: 2022-12
Effects of Lignin on the properties of reclaimed rubber /natural rubber blends
- Makhelema, Junith Matshidiso
- Authors: Makhelema, Junith Matshidiso
- Date: 2022-12
- Subjects: Lignin – South Africa , Tires – Recycling
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59744 , vital:62409
- Description: The environmental challenge posed by waste tyres is a serious global crisis from which South Africa is not isolated. The concept of tyre rubber reclamation into reusable rubber over the years has been on the increase, however, despite the environmental and economic advantages offered by the reclaiming process the main demerits of the resulting rubber include its relatively poor physical and mechanical properties when compared to newly manufactured rubber. The deterioration of these properties arises from the structural changes which occur during the reclaiming process. To overcome this disadvantage, reclaimed rubber can be blended with other rubbers such as natural rubber to compensate for its weak properties. However, there is stilll a limited industrial application of reclaimed/natural rubber blends due to their brittleness and relatively poor impact strength. By adding reinforcing fillers, the mechanical properties of the blends can be significantly improved. In recent years, there has been a renewed interest in the use of lignocellulosic materials as fillers, modifiers and reinforcements in polymeric materials. They are receiving attention because they are the most abundantly available raw materials on earth, and they offer greater versatility compared to inorganic fillers. Being the most abundant renewable agricultural derived lignocellulosic biomass after cellulose, and as far as polymer composites and blends are concerned, lignin has shown promise as a well-received and sustainable alternative to inorganic fillers due to its low-cost production and ability to improve on the biodegradability of polyolefins and minimise loss of ultimate mechanical properties of polymer matrices. The main aim of the research is to investigate the effect of lignin on the properties of reclaimed/natural rubber (RR/NR) blends for possible low-density and eco-friendly rubber materials. The motivation for the research arises from the global challenges that are connected to the dynamic increase of rubber wastes, especially in the form of used tyres and the potential availability of lignin from its annual production from the sugarcane pulp mills, which is regarded as waste. A mixture design of experiments was used to formulate different blends of lignin with reclaimed and natural rubber. The rubber blends and the lignin-rubber , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Makhelema, Junith Matshidiso
- Date: 2022-12
- Subjects: Lignin – South Africa , Tires – Recycling
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59744 , vital:62409
- Description: The environmental challenge posed by waste tyres is a serious global crisis from which South Africa is not isolated. The concept of tyre rubber reclamation into reusable rubber over the years has been on the increase, however, despite the environmental and economic advantages offered by the reclaiming process the main demerits of the resulting rubber include its relatively poor physical and mechanical properties when compared to newly manufactured rubber. The deterioration of these properties arises from the structural changes which occur during the reclaiming process. To overcome this disadvantage, reclaimed rubber can be blended with other rubbers such as natural rubber to compensate for its weak properties. However, there is stilll a limited industrial application of reclaimed/natural rubber blends due to their brittleness and relatively poor impact strength. By adding reinforcing fillers, the mechanical properties of the blends can be significantly improved. In recent years, there has been a renewed interest in the use of lignocellulosic materials as fillers, modifiers and reinforcements in polymeric materials. They are receiving attention because they are the most abundantly available raw materials on earth, and they offer greater versatility compared to inorganic fillers. Being the most abundant renewable agricultural derived lignocellulosic biomass after cellulose, and as far as polymer composites and blends are concerned, lignin has shown promise as a well-received and sustainable alternative to inorganic fillers due to its low-cost production and ability to improve on the biodegradability of polyolefins and minimise loss of ultimate mechanical properties of polymer matrices. The main aim of the research is to investigate the effect of lignin on the properties of reclaimed/natural rubber (RR/NR) blends for possible low-density and eco-friendly rubber materials. The motivation for the research arises from the global challenges that are connected to the dynamic increase of rubber wastes, especially in the form of used tyres and the potential availability of lignin from its annual production from the sugarcane pulp mills, which is regarded as waste. A mixture design of experiments was used to formulate different blends of lignin with reclaimed and natural rubber. The rubber blends and the lignin-rubber , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Evaluation of the mesozooplankton functional diversity indices in Algoa Bay as proxies to assess the stability of the food chain leading to the African penguin (Spheniscus demersus)
- Authors: Rukuni, Praxedes Vimbai
- Date: 2022-12
- Subjects: Marine zooplankton --South Africa -- Algoa bay , Penguins -- South Africa -- Conservation , Fishes -- Predators of -- South Africa -- Algoa Bay
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59956 , vital:62698
- Description: Current unprecedented environmental changes threaten food web and ecosystem stability, which is particularly concerning in areas with vulnerable species such as the endangered African penguin Spheniscus demersus. In this study I adopted a holistic approach integrating many ecosystem levels to better understand African penguin behavioural ecology and food web stability. I investigated interplay between physico-chemical parameters and zooplankton functional diversity at various temporal and spatial scales in Algoa Bay. The study further investigated the applicability of zooplankton functional diversity (FD) in inferring African penguin food web stability by comparing the FD of zooplankton found in anchovies regurgitated from African penguins and the ones found in the Bay PELTER Stations. Gut contents of anchovies regurgitated from Bird Island and St Croix penguins were also evaluated using Frequency of Occurrence and Dominance methods and compared between colonies. Zooplankton FD in Algoa Bay varied over time but not at spatial scales with chlorophyll-a, Sea Surface Temperature (SST), turbidity and salinity being potential drivers of zooplankton FD. Chlorophyll-a and salinity were positively correlated with FD, meanwhile FD was inversely correlated to turbidity and SST. The study also observed minor differences in the physico-chemical parameters associated with African penguin foraging grounds, though different dietary preferences were found between the anchovies regurgitated from penguins from the two colonies. Anchovies regurgitated by penguins from Bird Island targeted large sized groups such as Decapods, meanwhile anchovies from the St Croix penguin colony targeted small sized Ostracods and Cirripedia. This study therefore, provides insight on the influence of fluctuations in physico-chemical parameters in shaping zooplankton communities in Algoa Bay. The study also emphasises anchovy dietary plasticity, switching from selective feeding to opportunistic feeding in instances of prey shortages which contributes to a stable African penguin food web in the face of climate change. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Rukuni, Praxedes Vimbai
- Date: 2022-12
- Subjects: Marine zooplankton --South Africa -- Algoa bay , Penguins -- South Africa -- Conservation , Fishes -- Predators of -- South Africa -- Algoa Bay
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59956 , vital:62698
- Description: Current unprecedented environmental changes threaten food web and ecosystem stability, which is particularly concerning in areas with vulnerable species such as the endangered African penguin Spheniscus demersus. In this study I adopted a holistic approach integrating many ecosystem levels to better understand African penguin behavioural ecology and food web stability. I investigated interplay between physico-chemical parameters and zooplankton functional diversity at various temporal and spatial scales in Algoa Bay. The study further investigated the applicability of zooplankton functional diversity (FD) in inferring African penguin food web stability by comparing the FD of zooplankton found in anchovies regurgitated from African penguins and the ones found in the Bay PELTER Stations. Gut contents of anchovies regurgitated from Bird Island and St Croix penguins were also evaluated using Frequency of Occurrence and Dominance methods and compared between colonies. Zooplankton FD in Algoa Bay varied over time but not at spatial scales with chlorophyll-a, Sea Surface Temperature (SST), turbidity and salinity being potential drivers of zooplankton FD. Chlorophyll-a and salinity were positively correlated with FD, meanwhile FD was inversely correlated to turbidity and SST. The study also observed minor differences in the physico-chemical parameters associated with African penguin foraging grounds, though different dietary preferences were found between the anchovies regurgitated from penguins from the two colonies. Anchovies regurgitated by penguins from Bird Island targeted large sized groups such as Decapods, meanwhile anchovies from the St Croix penguin colony targeted small sized Ostracods and Cirripedia. This study therefore, provides insight on the influence of fluctuations in physico-chemical parameters in shaping zooplankton communities in Algoa Bay. The study also emphasises anchovy dietary plasticity, switching from selective feeding to opportunistic feeding in instances of prey shortages which contributes to a stable African penguin food web in the face of climate change. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Exploring the Methodologies Used by Construction Project Managers in South Africa
- Authors: Ferreira, Ruan
- Date: 2022-12
- Subjects: Construction -- South Africa , Project Management
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59381 , vital:62076
- Description: This study aimed to determine the prevalent project management methodologies (PMMs) are adopted within the South African construction industry. The insight obtained from this study will enhance awareness of the type of methodologies adopted by construction project managers. Moreover, through implementation of these methodologies, control can be enhanced, and the unknown managed. The predominant project management methodology used by construction project managers in South Africa is unknown. A quantitative research approach was used, and an online questionnaire was sent to the South African Council for the Project and Construction Management Professions (SACPCMP). The questionnaire was then administered and sent to the entire population of registered construction project managers (CPMs). The data from 103 respondents were captured onto a database and then statistically analysed to determine the predominant PMMs adopted by CPMs as well as their effect on project success. The study revealed that lean methodology is most widely adopted by CPMs, followed by the critical path method and Waterfall methodology. CPMs strive to reduce waste throughout the building process and organise that materials are only on site when required. Furthermore, a high level of PMM is adopted during the project life cycle phases. As a result, it can favourably impact projects and lead to project success. Adopting PMMs will result in improved control of project goals and scope, a shorter time of project execution, improved and efficient decision making, a higher degree of client satisfaction, and improved knowledge management and information exchange. , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Ferreira, Ruan
- Date: 2022-12
- Subjects: Construction -- South Africa , Project Management
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59381 , vital:62076
- Description: This study aimed to determine the prevalent project management methodologies (PMMs) are adopted within the South African construction industry. The insight obtained from this study will enhance awareness of the type of methodologies adopted by construction project managers. Moreover, through implementation of these methodologies, control can be enhanced, and the unknown managed. The predominant project management methodology used by construction project managers in South Africa is unknown. A quantitative research approach was used, and an online questionnaire was sent to the South African Council for the Project and Construction Management Professions (SACPCMP). The questionnaire was then administered and sent to the entire population of registered construction project managers (CPMs). The data from 103 respondents were captured onto a database and then statistically analysed to determine the predominant PMMs adopted by CPMs as well as their effect on project success. The study revealed that lean methodology is most widely adopted by CPMs, followed by the critical path method and Waterfall methodology. CPMs strive to reduce waste throughout the building process and organise that materials are only on site when required. Furthermore, a high level of PMM is adopted during the project life cycle phases. As a result, it can favourably impact projects and lead to project success. Adopting PMMs will result in improved control of project goals and scope, a shorter time of project execution, improved and efficient decision making, a higher degree of client satisfaction, and improved knowledge management and information exchange. , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2022
- Full Text:
- Date Issued: 2022-12
Factors that impact the use of digital learning in higher education
- Authors: Mtshabe Mxolisi
- Date: 2022-12
- Subjects: Education, Higher , Blended learning , Communication of technical information
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59576 , vital:62174
- Description: Higher Education Institutions (HEIs) have significantly changed the way they execute their pedagogical processes. This is so, mainly because of the innovations and developments in the ICT sector. HEIs have always succeeded in the implementation of ICT tools for the facilitation of the process of teaching and learning; however, they have faced many challenges related to the utilisation of these tools. Digital learning is among the ICT tools considered by the majority of HEIs to be relevant at this age of digitisation. ICT has introduced a variety of tools, not only for teaching and learning environments, but also for socialisation. These tools include social media tools that are used to achieve different activities in response to the demands of society. In making a comparison between social media and pedagogical tools, there is a huge gap in the level of usage of the two. Social media is much more being used than digital learning and this brings major concerns because users seem to be more interested in social media tools to perform academic activities, than in digital learning. This study has explored the factors that could play a role in digital learning and social media usage in HEIs. The study employed quantitative research methods and a survey was developed and distributed to former university students. The purpose of the study was to formulate a checklist that will inform the factors that impact the use of digital learning in HEIs. At the conclusion of the study, the checklist was formulated and recommendations were made. , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Mtshabe Mxolisi
- Date: 2022-12
- Subjects: Education, Higher , Blended learning , Communication of technical information
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59576 , vital:62174
- Description: Higher Education Institutions (HEIs) have significantly changed the way they execute their pedagogical processes. This is so, mainly because of the innovations and developments in the ICT sector. HEIs have always succeeded in the implementation of ICT tools for the facilitation of the process of teaching and learning; however, they have faced many challenges related to the utilisation of these tools. Digital learning is among the ICT tools considered by the majority of HEIs to be relevant at this age of digitisation. ICT has introduced a variety of tools, not only for teaching and learning environments, but also for socialisation. These tools include social media tools that are used to achieve different activities in response to the demands of society. In making a comparison between social media and pedagogical tools, there is a huge gap in the level of usage of the two. Social media is much more being used than digital learning and this brings major concerns because users seem to be more interested in social media tools to perform academic activities, than in digital learning. This study has explored the factors that could play a role in digital learning and social media usage in HEIs. The study employed quantitative research methods and a survey was developed and distributed to former university students. The purpose of the study was to formulate a checklist that will inform the factors that impact the use of digital learning in HEIs. At the conclusion of the study, the checklist was formulated and recommendations were made. , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2022
- Full Text:
- Date Issued: 2022-12
Fixed point theory in metric and normed Spaces
- Authors: Naude, Luan
- Date: 2022-12
- Subjects: Metric spaces – South Africa , Banach spaces – South Africa , Mappings (Mathematics)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59898 , vital:62686
- Description: In this dissertation, we present major results in the theory of fixed points in metric and normed spaces. We start with a review of the Banach fixed point theorem and some of its applications (in systems of linear equations, differential equations, integral equations, and dynamical systems), and then discuss many of its extensions and generalizations. We look at the theorem of Edelstein ([8, Remark 3.1]) in compact metric spaces, and a generalizaton of it proved by Suzuki ([19, Theorem 3]) in 2009. We then give a detailed account of the work of Meyers: In [11], Meyers proved generalizations of the Banach fixed point theorem to uniform local contractions, and, in [10], a converse to the Banach fixed point theorem. Finally, we look at some of Browder’s work in fixed point theory. In [6], he showed the existence of fixed points for nonexpansive mappings on bounded, closed, and convex sets in uniformly convex Banach spaces, and, in [5], he proved similar results in Hilbert spaces using a connection between nonexpansive mappings and monotone operators. Keywords: Fixed point, Functional analysis, Metric spaces, Banach spaces, Hilbert spaces, contractions, Banach fixed point theorem, nonexpansive mappings. , Thesis (MSc) -- Faculty of Science, School of Computer Science, Mathematics, Physics and Statistics, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Naude, Luan
- Date: 2022-12
- Subjects: Metric spaces – South Africa , Banach spaces – South Africa , Mappings (Mathematics)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59898 , vital:62686
- Description: In this dissertation, we present major results in the theory of fixed points in metric and normed spaces. We start with a review of the Banach fixed point theorem and some of its applications (in systems of linear equations, differential equations, integral equations, and dynamical systems), and then discuss many of its extensions and generalizations. We look at the theorem of Edelstein ([8, Remark 3.1]) in compact metric spaces, and a generalizaton of it proved by Suzuki ([19, Theorem 3]) in 2009. We then give a detailed account of the work of Meyers: In [11], Meyers proved generalizations of the Banach fixed point theorem to uniform local contractions, and, in [10], a converse to the Banach fixed point theorem. Finally, we look at some of Browder’s work in fixed point theory. In [6], he showed the existence of fixed points for nonexpansive mappings on bounded, closed, and convex sets in uniformly convex Banach spaces, and, in [5], he proved similar results in Hilbert spaces using a connection between nonexpansive mappings and monotone operators. Keywords: Fixed point, Functional analysis, Metric spaces, Banach spaces, Hilbert spaces, contractions, Banach fixed point theorem, nonexpansive mappings. , Thesis (MSc) -- Faculty of Science, School of Computer Science, Mathematics, Physics and Statistics, 2022
- Full Text:
- Date Issued: 2022-12
Growth performance of three F1 Hybrid tomato (Solanum lycopersicum L.) Cultivars grown in Peat moss and Sawdust
- Authors: Mondile, Landile
- Date: 2022-12
- Subjects: Agricultural development projects -- South Africa -- Eastern Cape , Tomatoes – Irrigation – Management
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59853 , vital:62450
- Description: The tomato is one of the most important and commonly grown vegetable crops cultivated by subsistence as well as commercial farmers in South Africa. Even though hydroponic tomato production has gained popularity, tomato production in South Africa is mostly practised open field. This is because most South African households in rural communities depend on agriculture for their livelihoods. Tomatoes are one of the “cash crops” − meaning cash in hand for many farmers. As a staple food for many rural communities and households, tomatoes are prepared as relish daily. Including tomatoes in the human diet provides the benefit of various important nutrients, such as Vitamin C, amongst others. The main aim of this study was to evaluate the growth performance − by assessing the number of leaves, stem diameter, plant height and number of flowers per plant − in three Solanum lycopersicum L. cultivars, namely: F1 hybrids Star9006, TF4009 and TF4011, grown in peat moss and sawdust respectively. In addition, the research aimed at investigating how peat moss and sawdust, as growing media, influenced the growth performance of the tomato cultivars under study. The study was conducted at the Döhne Agricultural Development Institute near Stutterheim in the Eastern Cape. The experiment was laid out in a split randomized complete block design (RCBD) in which the growing media, namely: sawdust and peat moss, were assigned as main plots and the tomato cultivars to sub-plots, with three replicates. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Mondile, Landile
- Date: 2022-12
- Subjects: Agricultural development projects -- South Africa -- Eastern Cape , Tomatoes – Irrigation – Management
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59853 , vital:62450
- Description: The tomato is one of the most important and commonly grown vegetable crops cultivated by subsistence as well as commercial farmers in South Africa. Even though hydroponic tomato production has gained popularity, tomato production in South Africa is mostly practised open field. This is because most South African households in rural communities depend on agriculture for their livelihoods. Tomatoes are one of the “cash crops” − meaning cash in hand for many farmers. As a staple food for many rural communities and households, tomatoes are prepared as relish daily. Including tomatoes in the human diet provides the benefit of various important nutrients, such as Vitamin C, amongst others. The main aim of this study was to evaluate the growth performance − by assessing the number of leaves, stem diameter, plant height and number of flowers per plant − in three Solanum lycopersicum L. cultivars, namely: F1 hybrids Star9006, TF4009 and TF4011, grown in peat moss and sawdust respectively. In addition, the research aimed at investigating how peat moss and sawdust, as growing media, influenced the growth performance of the tomato cultivars under study. The study was conducted at the Döhne Agricultural Development Institute near Stutterheim in the Eastern Cape. The experiment was laid out in a split randomized complete block design (RCBD) in which the growing media, namely: sawdust and peat moss, were assigned as main plots and the tomato cultivars to sub-plots, with three replicates. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Habitat use by Juvenile coastal fish in subtidal vegetated habitats of Algoa Bay;s shallow water seascape
- Authors: Mkhize,Thembani
- Date: 2022-12
- Subjects: Sailing ships -- South Africa -- Algoa Bay --History , Marine fishes -- Algoa Bay -- South Africa , Fish improvement habitat
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59788 , vital:62429
- Description: Structurally complex habitats such as seagrass, mangroves, and seaweed are important as nurseries in estuaries and nearshore marine environments. While numerous studies have focussed on structurally complex vegetated habitats in estuaries, few studies have focussed on vegetated nursery habitats (particularly seaweed) in coastal areas and their importance. The main aim of this project is to examine the nature of shallow water vegetated habitat (Zostera capensis seagrass and Plocamium corallorhiza macroalga) usage by juvenile fish in the Algoa Bay shallow water seascape (Swartkops Estuary and Flat Rocks nearshore subtidal reef). This aim was achieved by quantifying and comparing complexity of these two habitat-forming species, and the fish assemblages (relative abundance, richness, size structure and behaviour) across the two habitats. To measure and compare complexity of Z. capensis and P. corallorhiza, canopy height, density and leaf/blade width were sampled. In addition to these structural complexity indices, Fractal D (dimensionless complexity indices) was also quantified using Image software. Zostera capensis (1553.3 Indiv/m2 ) had a slightly higher mean density compared to P. corallorhiza (1303.7 Indiv/m2 ) but the difference was not significant. When sites (within each habitat) were compared, density did not show any significant differences between sites. Canopy height of Z. capensis (56.9 cm) was significantly higher compared to P. corallorhiza (16.6 cm), with no significant differences between sites. On the other hand, P. corallorhiza (0.9 cm) blades were significantly wider than Z. capensis (0.2 cm) leaves. Fractal D values followed the same trend as leaf/blade width with, with P. corallorhiza (1.8) having significantly higher Fractal D values than Z. capensis (1.5). The last two indices also had a positive relationship as Fractal D increased with increasing leaf/blade width. These results show that overall P. corallorhiza is more structurally complex than Z. capensis. Calibrated mini stereo underwater remote video systems (mini stereo-RUVs) were optimised in these two studied habitats to ensure they were suitable to measure and identify juvenile fishes. A pilot optimization study found that a minimum of five deployments and a minimum of 45 minutes filming duration is required to study fish assemblages in these two habitats. For comparison of fish assemblages and assessment of habitat use by fish, mini stereo-RUVs were deployed in both habitats (three sites per habitat) and were left to record for 60 minutes for a v total of five sampling occasions in seagrass and seven sampling occasions in macroalga between 7 September 2020 and 9 April 2021. Collected videos were analysed, with relative abundance (MaxN), richness and fish length extracted. Fish behaviour was also analysed and compared between habitats. Mean fish MaxN was slightly higher in Z. capensis (3.4) than in P. corallorhiza (2.9), although this result was not significant. Species diversity was significantly higher in P. corallorhiza as shown by both richness (Z. capensis = 11 and P. corallorhiza = 18) and the Shannon diversity index (Z. capensis = 1.3 and P. corallorhiza = 1.7). Both habitats were dominated by species from the family Sparidae, with six estuary-associated marine species common between the two habitats. Both Z. capensis and P. corallorhiza were dominated by juvenile fish, with more than 70 % of measured fish being juveniles. Fish length was not significantly different in the two habitats. Juvenile fish assemblages were significantly distinct between the two habitats (ANOSIM). In terms of habitat use, fish behaviour analysis showed that fish use both habitats as nurseries. There was no significant difference in fish behaviour in the two habitats and fish behaviours associated with higher levels of habitat use (slow meandering and feeding) were the most common behaviours in both habitats. Although not significant, feeding behaviour (also associated with a high degree of habitat use) was observed more in macroalga than in the seagrass habitat. Although none of the studied complexity indices could be related to abundance, both leaf/blade width and Fractal Dimension showed a positive relationship with the number of species recorded, while canopy height showed a strong negative relationship with the number of species. Overall, this study shows that Plocamium corallorhiza red macroalga in the shallow marine environment may be as important in shallow marine environments as Zostera capensis seagrass in estuaries as nursery habitats for marine fi , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Mkhize,Thembani
- Date: 2022-12
- Subjects: Sailing ships -- South Africa -- Algoa Bay --History , Marine fishes -- Algoa Bay -- South Africa , Fish improvement habitat
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59788 , vital:62429
- Description: Structurally complex habitats such as seagrass, mangroves, and seaweed are important as nurseries in estuaries and nearshore marine environments. While numerous studies have focussed on structurally complex vegetated habitats in estuaries, few studies have focussed on vegetated nursery habitats (particularly seaweed) in coastal areas and their importance. The main aim of this project is to examine the nature of shallow water vegetated habitat (Zostera capensis seagrass and Plocamium corallorhiza macroalga) usage by juvenile fish in the Algoa Bay shallow water seascape (Swartkops Estuary and Flat Rocks nearshore subtidal reef). This aim was achieved by quantifying and comparing complexity of these two habitat-forming species, and the fish assemblages (relative abundance, richness, size structure and behaviour) across the two habitats. To measure and compare complexity of Z. capensis and P. corallorhiza, canopy height, density and leaf/blade width were sampled. In addition to these structural complexity indices, Fractal D (dimensionless complexity indices) was also quantified using Image software. Zostera capensis (1553.3 Indiv/m2 ) had a slightly higher mean density compared to P. corallorhiza (1303.7 Indiv/m2 ) but the difference was not significant. When sites (within each habitat) were compared, density did not show any significant differences between sites. Canopy height of Z. capensis (56.9 cm) was significantly higher compared to P. corallorhiza (16.6 cm), with no significant differences between sites. On the other hand, P. corallorhiza (0.9 cm) blades were significantly wider than Z. capensis (0.2 cm) leaves. Fractal D values followed the same trend as leaf/blade width with, with P. corallorhiza (1.8) having significantly higher Fractal D values than Z. capensis (1.5). The last two indices also had a positive relationship as Fractal D increased with increasing leaf/blade width. These results show that overall P. corallorhiza is more structurally complex than Z. capensis. Calibrated mini stereo underwater remote video systems (mini stereo-RUVs) were optimised in these two studied habitats to ensure they were suitable to measure and identify juvenile fishes. A pilot optimization study found that a minimum of five deployments and a minimum of 45 minutes filming duration is required to study fish assemblages in these two habitats. For comparison of fish assemblages and assessment of habitat use by fish, mini stereo-RUVs were deployed in both habitats (three sites per habitat) and were left to record for 60 minutes for a v total of five sampling occasions in seagrass and seven sampling occasions in macroalga between 7 September 2020 and 9 April 2021. Collected videos were analysed, with relative abundance (MaxN), richness and fish length extracted. Fish behaviour was also analysed and compared between habitats. Mean fish MaxN was slightly higher in Z. capensis (3.4) than in P. corallorhiza (2.9), although this result was not significant. Species diversity was significantly higher in P. corallorhiza as shown by both richness (Z. capensis = 11 and P. corallorhiza = 18) and the Shannon diversity index (Z. capensis = 1.3 and P. corallorhiza = 1.7). Both habitats were dominated by species from the family Sparidae, with six estuary-associated marine species common between the two habitats. Both Z. capensis and P. corallorhiza were dominated by juvenile fish, with more than 70 % of measured fish being juveniles. Fish length was not significantly different in the two habitats. Juvenile fish assemblages were significantly distinct between the two habitats (ANOSIM). In terms of habitat use, fish behaviour analysis showed that fish use both habitats as nurseries. There was no significant difference in fish behaviour in the two habitats and fish behaviours associated with higher levels of habitat use (slow meandering and feeding) were the most common behaviours in both habitats. Although not significant, feeding behaviour (also associated with a high degree of habitat use) was observed more in macroalga than in the seagrass habitat. Although none of the studied complexity indices could be related to abundance, both leaf/blade width and Fractal Dimension showed a positive relationship with the number of species recorded, while canopy height showed a strong negative relationship with the number of species. Overall, this study shows that Plocamium corallorhiza red macroalga in the shallow marine environment may be as important in shallow marine environments as Zostera capensis seagrass in estuaries as nursery habitats for marine fi , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Hooked on Gqom: an ethnographic study of a contemporary urban youth musical identity in South Africa
- Katushabe,Bathandwa Mathias Ngasiirwe
- Authors: Katushabe,Bathandwa Mathias Ngasiirwe
- Date: 2022-12
- Subjects: Electric dance Music -- South Africa -- Durban , Kwaito Music -- Social Ascpects -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59326 , vital:62045
- Description: Gqom music has grown from being a relatively obscure sub-genre of South African Kwaito into a globally recognised sonic identity and social movement. Gqom, as a distinct music style, has featured heavily in mass media since 2015, spawning social phenomena such as viral dance challenges on various social media platforms, and constituting a launch pad for the careers of musicians such as DJ Lag and the Distruction Boyz. Gqom also featured in the international motion picture Black Panther in 2018, and in the 2019 Lion King: The Gift album by American pop icon Beyoncé. Although the origins of Gqom, its characteristics, impact, and ascension to noteworthiness have received attention in the form of newspaper articles, online blogs, YouTube documentaries, etc, and small treatises or academic articles, little literature has been generated about the music and its theoretical construction as sonic and social phenomena. I argue that by using the theoretical scaffolds of ‘identity politics’, specifically ‘identities in music’, one is better able to understand Gqom as reflective of a broader social urban youth identity characterised by partying and political disillusionment. In other words, I make the case for an intergroup identification that prioritises in-group musical identities associated with Gqom and its phenomenology of practices which I have excavated using an ethnographic qualitative method. , Thesis (MMus) -- Faculty of Humanities, School of Visual and performing Arts, 2022
- Full Text:
- Date Issued: 2022-12
Hooked on Gqom: an ethnographic study of a contemporary urban youth musical identity in South Africa
- Authors: Katushabe,Bathandwa Mathias Ngasiirwe
- Date: 2022-12
- Subjects: Electric dance Music -- South Africa -- Durban , Kwaito Music -- Social Ascpects -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59326 , vital:62045
- Description: Gqom music has grown from being a relatively obscure sub-genre of South African Kwaito into a globally recognised sonic identity and social movement. Gqom, as a distinct music style, has featured heavily in mass media since 2015, spawning social phenomena such as viral dance challenges on various social media platforms, and constituting a launch pad for the careers of musicians such as DJ Lag and the Distruction Boyz. Gqom also featured in the international motion picture Black Panther in 2018, and in the 2019 Lion King: The Gift album by American pop icon Beyoncé. Although the origins of Gqom, its characteristics, impact, and ascension to noteworthiness have received attention in the form of newspaper articles, online blogs, YouTube documentaries, etc, and small treatises or academic articles, little literature has been generated about the music and its theoretical construction as sonic and social phenomena. I argue that by using the theoretical scaffolds of ‘identity politics’, specifically ‘identities in music’, one is better able to understand Gqom as reflective of a broader social urban youth identity characterised by partying and political disillusionment. In other words, I make the case for an intergroup identification that prioritises in-group musical identities associated with Gqom and its phenomenology of practices which I have excavated using an ethnographic qualitative method. , Thesis (MMus) -- Faculty of Humanities, School of Visual and performing Arts, 2022
- Full Text:
- Date Issued: 2022-12
Implementation framework for microgrids as an energy solution to uplift rural communities in the Eastern Cape
- Authors: Sibulelokuhle Xulaba
- Date: 2022-12
- Subjects: Renewable energy sources -- Eastern Cape , Microgrids (Smart power grids) -- Eastern Cape
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/60071 , vital:62916
- Description: People in remote areas of most developing countries still face economic and environmental challenges despite our more accessible world of technology. Access to reliable and sustainable electricity is the most challenging developmental issue faced by rural communities in South Africa, as grid expansion has dwindled due to business challenges experiences by the state power producer, Eskom. Unless businesses and homeowners in unconnected areas use generators, which are costly to run and environmentally unfriendly, most will continue to remain without electricity for the foreseeable future. The purpose of this study therefore is to provide a model for the successful implementation of renewable energy microgrids to overcome poverty and promote economic development in rural areas of the Eastern Cape. The data for the study was collected from three district municipalities in the rural areas of the Eastern Cape. The province is divided into homeland and farms, with the farms that were sampled for the study situated on State-owned land. The sample group comprised small businesses operating in these rural areas. The study used a mixed-method research design, the methodology being found to be the most suitable for the study. The study uses a questionnaire-variant convergent design that consisted of a mixture of open-ended and close-ended questions. The sample group was situated in areas with limited electricity or internet access, therefore face-toface structured interviews were conducted. Meta-inference was used for data interpretation, and a combination of quantitative and qualitative data analysis methods was used to analyse the data. For the open-ended questions, thematic analysis was used, whilst descriptive statistics were used for the closed-ended questions. The study found that most businesses operating in rural areas do not have access to electricity. Those businesses which do not have electricity must make use of diesel-powered generators to continue to trade, which makes their businesses unprofitable. They spend a large proportion of their earnings purchasing fuel to achieve the desire thermal comfort for their stock, or to pump water via boreholes or piped dam structures. The study showed that implementing a renewable energy microgrids provides a viable option, supporting the literature reviewed. This will promote economic development in these areas of the Eastern Cape. Developing and enhancing the standard of living can assist in reducing the number of people migrating to urban areas whilst providing an opportunity to increase farm yields, grow rural business and change the lives of the poor for the better. , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Sibulelokuhle Xulaba
- Date: 2022-12
- Subjects: Renewable energy sources -- Eastern Cape , Microgrids (Smart power grids) -- Eastern Cape
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/60071 , vital:62916
- Description: People in remote areas of most developing countries still face economic and environmental challenges despite our more accessible world of technology. Access to reliable and sustainable electricity is the most challenging developmental issue faced by rural communities in South Africa, as grid expansion has dwindled due to business challenges experiences by the state power producer, Eskom. Unless businesses and homeowners in unconnected areas use generators, which are costly to run and environmentally unfriendly, most will continue to remain without electricity for the foreseeable future. The purpose of this study therefore is to provide a model for the successful implementation of renewable energy microgrids to overcome poverty and promote economic development in rural areas of the Eastern Cape. The data for the study was collected from three district municipalities in the rural areas of the Eastern Cape. The province is divided into homeland and farms, with the farms that were sampled for the study situated on State-owned land. The sample group comprised small businesses operating in these rural areas. The study used a mixed-method research design, the methodology being found to be the most suitable for the study. The study uses a questionnaire-variant convergent design that consisted of a mixture of open-ended and close-ended questions. The sample group was situated in areas with limited electricity or internet access, therefore face-toface structured interviews were conducted. Meta-inference was used for data interpretation, and a combination of quantitative and qualitative data analysis methods was used to analyse the data. For the open-ended questions, thematic analysis was used, whilst descriptive statistics were used for the closed-ended questions. The study found that most businesses operating in rural areas do not have access to electricity. Those businesses which do not have electricity must make use of diesel-powered generators to continue to trade, which makes their businesses unprofitable. They spend a large proportion of their earnings purchasing fuel to achieve the desire thermal comfort for their stock, or to pump water via boreholes or piped dam structures. The study showed that implementing a renewable energy microgrids provides a viable option, supporting the literature reviewed. This will promote economic development in these areas of the Eastern Cape. Developing and enhancing the standard of living can assist in reducing the number of people migrating to urban areas whilst providing an opportunity to increase farm yields, grow rural business and change the lives of the poor for the better. , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2022
- Full Text:
- Date Issued: 2022-12
Improving governance and service delivery in Nelson Mandela Metropolitan Municipality: a quest for a clean audit opinion
- Ntsundwana, Kanyisa Zime Dadewabobonke
- Authors: Ntsundwana, Kanyisa Zime Dadewabobonke
- Date: 2022-12
- Subjects: Service delivery -- Nelson Mandela Metropolitan Municipality -- Port Elizabeth , Municipal finance--Auditing--Law and legislation
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59644 , vital:62297
- Description: The purpose of the study was to investigate how Nelson Mandela Bay Metropolitan Municipality (NMBMM) can improve its governance and service delivery to obtain a clean audit outcome. In addition, the study explored the role of auditing in the functioning and performance of municipalities. NMBMM public officials and political office-bearers are expected in terms of Chapter 7 of the Constitution of South Africa Act 108 of 1996 to promote the general welfare of the community by meeting its needs, expectations and demands. The qualitative (non-empirical) research methodology followed in the study was based on a desktop approach. The annual documents reviewed to answer the research objectives were the Consolidated General Reports on the Local Government Audit Outcome and the Reports of the Auditor-General to the Eastern Cape Provincial Legislature and the council on the NMBMM and its municipal entity for the financial period 2015/2016 to 2019/2020. The problem was that the NMBMM did not obtain a clean audit opinion during this period. The study findings indicated that the main contributing factors that prevented the NMBMM from improving governance, service delivery and obtaining a clean audit outcome were as follows: poor management of accounting practices (financial management); inadequate financial statements and performance reports; a lack of adherence to predetermined objectives and legislation; and unauthorised, fruitless, wasteful and irregular expenditure. To attain a clean audit outcome, NMBMM municipal officials and political officebearers need to perform their mandated duties diligently and overcome the challenges that have barred them from obtaining an unqualified audit opinion. Such an endeavour would be consistent with the Operation Clean Audit (OPCA) campaign, which was introduced by the former Minister of Cooperative Governance and Traditional Affairs, Sicelo Shiceka, in 2009. In other words, the NMBMM must improve its governance and service delivery because a municipality without good governance lacks accountability, which has a direct negative impact on the delivery of services to its communities. Hence, it is recommended inter alia that the NMBMM seeks advice from iii the Auditor-General on strategies to improve its audit outcomes, maintains infrastructure, addresses criminal activities and improves its internal controls. , Thesis (MSc) -- Faculty of Humanities, School of Governmental and Social sciences, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Ntsundwana, Kanyisa Zime Dadewabobonke
- Date: 2022-12
- Subjects: Service delivery -- Nelson Mandela Metropolitan Municipality -- Port Elizabeth , Municipal finance--Auditing--Law and legislation
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59644 , vital:62297
- Description: The purpose of the study was to investigate how Nelson Mandela Bay Metropolitan Municipality (NMBMM) can improve its governance and service delivery to obtain a clean audit outcome. In addition, the study explored the role of auditing in the functioning and performance of municipalities. NMBMM public officials and political office-bearers are expected in terms of Chapter 7 of the Constitution of South Africa Act 108 of 1996 to promote the general welfare of the community by meeting its needs, expectations and demands. The qualitative (non-empirical) research methodology followed in the study was based on a desktop approach. The annual documents reviewed to answer the research objectives were the Consolidated General Reports on the Local Government Audit Outcome and the Reports of the Auditor-General to the Eastern Cape Provincial Legislature and the council on the NMBMM and its municipal entity for the financial period 2015/2016 to 2019/2020. The problem was that the NMBMM did not obtain a clean audit opinion during this period. The study findings indicated that the main contributing factors that prevented the NMBMM from improving governance, service delivery and obtaining a clean audit outcome were as follows: poor management of accounting practices (financial management); inadequate financial statements and performance reports; a lack of adherence to predetermined objectives and legislation; and unauthorised, fruitless, wasteful and irregular expenditure. To attain a clean audit outcome, NMBMM municipal officials and political officebearers need to perform their mandated duties diligently and overcome the challenges that have barred them from obtaining an unqualified audit opinion. Such an endeavour would be consistent with the Operation Clean Audit (OPCA) campaign, which was introduced by the former Minister of Cooperative Governance and Traditional Affairs, Sicelo Shiceka, in 2009. In other words, the NMBMM must improve its governance and service delivery because a municipality without good governance lacks accountability, which has a direct negative impact on the delivery of services to its communities. Hence, it is recommended inter alia that the NMBMM seeks advice from iii the Auditor-General on strategies to improve its audit outcomes, maintains infrastructure, addresses criminal activities and improves its internal controls. , Thesis (MSc) -- Faculty of Humanities, School of Governmental and Social sciences, 2022
- Full Text:
- Date Issued: 2022-12
In vitro evaluation of vernonia amygdalina gold nanoparticles to stimulate apoptosis in breast cancer lines
- Authors: Pali, Zenande
- Date: 2022-12
- Subjects: Breast--Cancer--Treatment--Technological innovations , Apoptosis -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59933 , vital:62696
- Description: Breast cancer is the most prevalent cancer in women and triple negative breast cancer (TNBC) is one of the most aggressive breast cancer subtypes. Current breast cancer therapies are associated with several side effects and less effective treatment. Therefore, the development of new approaches to improve cancer treatment are needed. The use of gold nanoparticles (AuNPs) for anti-cancer effects is one of the most promising research areas in the field of nanotechnology, because nanoparticles are readily functionalized and can passively accumulate at the tumor site without affecting healthy tissues. The green synthesis of AuNPs is an eco-friendly, costeffective, fast, and non-toxic method that is associated with fewer side effects.The aim of this study was to evaluate the cytotoxicity and apoptotic activity exhibted by Vernonia amygdalina gold nanoparticles (VA-AuNPs) in triple negative breast cancer cell lines. The aqueous extract of Vernonia amygdalina was used to reduce gold salts to form VA-AuNPs. The VA-AuNPs were synthesised at a temperature of 100 °C and pH 7. The synthesised green AuNPs were charactised using ultraviolet-visible spectroscopy, dynamic light scattering, and high resolution transmission electron microscopy. Spherical VA-AuNPs were succesfully synthesized with average size of 17.11 ± 2.82 nm and a zeta potential of -36.55 ± 1.15 mV. The MTT assay revealed that VA-AuNPs significantly reduced (p cell viability for both mouse (E0771) and human (MDA-MB231) TNBC cell lines in a dose-dependent manner. The PrestoBlue assay showed a consistent decrease in cell numbers over time, suggesting either a decrease in proliferation or increase in apoptosis. The apoptotic effects of VAAuNPs were assessed using the CaspGLOW Fluorescein active caspase-8 staining kit. It indicated that caspase 8 was activated when cells were treated with VA-AuNPs. E0771 and MDA-MB231 cells were compared when treated with VA-AuNP and a similar trend was established. The HRTEM image showed VA-AuNPs in an vesiclelike structure inside the cell. It can be concluded that the VA-AuNPs showed anticancer activities against TNBC cells. Caspase 8 is activated by VA-AuNPs, confirming the induction of apoptosis, but does not exclude the activation of the intrinsic apoptotic pathway or inhibition of proliferation activity. E0771 and MDA-MB231 cells showed similar cytotoxic activity and thus expected that the mechanism of action in these cell lines should also be similar. Future in vivo research, using the mouse model inoculated with E0771 TNBC cells, should be justified and comparable with human TNBC cells. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Pali, Zenande
- Date: 2022-12
- Subjects: Breast--Cancer--Treatment--Technological innovations , Apoptosis -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59933 , vital:62696
- Description: Breast cancer is the most prevalent cancer in women and triple negative breast cancer (TNBC) is one of the most aggressive breast cancer subtypes. Current breast cancer therapies are associated with several side effects and less effective treatment. Therefore, the development of new approaches to improve cancer treatment are needed. The use of gold nanoparticles (AuNPs) for anti-cancer effects is one of the most promising research areas in the field of nanotechnology, because nanoparticles are readily functionalized and can passively accumulate at the tumor site without affecting healthy tissues. The green synthesis of AuNPs is an eco-friendly, costeffective, fast, and non-toxic method that is associated with fewer side effects.The aim of this study was to evaluate the cytotoxicity and apoptotic activity exhibted by Vernonia amygdalina gold nanoparticles (VA-AuNPs) in triple negative breast cancer cell lines. The aqueous extract of Vernonia amygdalina was used to reduce gold salts to form VA-AuNPs. The VA-AuNPs were synthesised at a temperature of 100 °C and pH 7. The synthesised green AuNPs were charactised using ultraviolet-visible spectroscopy, dynamic light scattering, and high resolution transmission electron microscopy. Spherical VA-AuNPs were succesfully synthesized with average size of 17.11 ± 2.82 nm and a zeta potential of -36.55 ± 1.15 mV. The MTT assay revealed that VA-AuNPs significantly reduced (p cell viability for both mouse (E0771) and human (MDA-MB231) TNBC cell lines in a dose-dependent manner. The PrestoBlue assay showed a consistent decrease in cell numbers over time, suggesting either a decrease in proliferation or increase in apoptosis. The apoptotic effects of VAAuNPs were assessed using the CaspGLOW Fluorescein active caspase-8 staining kit. It indicated that caspase 8 was activated when cells were treated with VA-AuNPs. E0771 and MDA-MB231 cells were compared when treated with VA-AuNP and a similar trend was established. The HRTEM image showed VA-AuNPs in an vesiclelike structure inside the cell. It can be concluded that the VA-AuNPs showed anticancer activities against TNBC cells. Caspase 8 is activated by VA-AuNPs, confirming the induction of apoptosis, but does not exclude the activation of the intrinsic apoptotic pathway or inhibition of proliferation activity. E0771 and MDA-MB231 cells showed similar cytotoxic activity and thus expected that the mechanism of action in these cell lines should also be similar. Future in vivo research, using the mouse model inoculated with E0771 TNBC cells, should be justified and comparable with human TNBC cells. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-12