Development of an in-situ ß-D-Glucuronidase diagnostic moraxella-based biosensor for potential application in the monitoring of water polluted by faecal material in South Africa
- Authors: Togo, Chamunorwa Aloius
- Date: 2007
- Subjects: Water quality management -- South Africa Water quality bioassay -- South Africa Sewage sludge -- South Africa -- Management Water -- Purification -- Biological treatment -- South Africa Biosensors
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3947 , http://hdl.handle.net/10962/d1004006
- Description: The prevention of outbreaks of waterborne diseases remains a major challenge to public health service providers globally. One of the major obstacles in this effort is the unavailability of on-line and real-time methods for rapid monitoring of faecal pollution to facilitate early warning of contamination of drinking water. The main objective of this study was to develop a β-glucuronidase (GUD)-based method that could be used for the on-line and real-time monitoring of microbial water quality. GUD is a marker enzyme for the faecal indicator bacteria Escherichia coli. This enzyme breaks down the synthetic substrate p-nitrophenyl-β-D-glucuronide (PNPG) to D-glucuronic acid and p-nitrophenol (PNP), which turns yellow under alkaline pH. The enzymatically produced PNP was used to detect GUD activity. In situ GUD assays were performed using running and stagnant water samples from the Bloukrans River, Grahamstown, South Africa. The physico-chemical properties of environmental GUD were determined, after which a liquid bioprobe and a microbial biosensor modified with Moraxella 1A species for the detection of the enzyme activity were developed. In order to determine the reliability and sensitivity of these methods, regression analyses for each method versus E. coli colony forming units (CFU) were performed. The storage stabilities of the bioprobe and biosensor were also investigated. The physico-chemical properties of in situ GUD were different from those of its commercially available counterpart. The temperature optimum for the former was between 35 and 40 °C while for the latter it was 45 °C. Commercial (reference) GUD had a pH optimum of 8.0 while the environmental counterpart exhibited a broad pH optimum of between pH 5.0 and 8.0. The liquid bioprobe had a limit of detection (LOD) of GUD activity equivalent to 2 CFU/100 ml and a detection time of 24 h. The method was less labour intensive and costly than the culturing method. The liquid bioprobe was stable for at least four weeks at room temperature (20 ± 2 °C). The biosensor was prepared by modifying a glassy carbon electrode with PNP degrading Moraxella 1A cells. The biosensor was 100 times more sensitive and rapid (5-20 min) than the spectrophotometric method (24 h), and was also able to detect GUD activity of viable but non-culturable cells. Thus it was more sensitive than the culturing method. Furthermore, the biosensor was selective and costeffective. The possibility of using a Pseudomonas putida JS444 biosensor was also investigated, but it was not as sensitive and selective as the Moraxella 1A biosensor. The Moraxella biosensor, therefore, offered the best option for on-line and real-time microbial water quality monitoring in South African river waters and drinking water supplies.
- Full Text:
- Date Issued: 2007
- Authors: Togo, Chamunorwa Aloius
- Date: 2007
- Subjects: Water quality management -- South Africa Water quality bioassay -- South Africa Sewage sludge -- South Africa -- Management Water -- Purification -- Biological treatment -- South Africa Biosensors
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3947 , http://hdl.handle.net/10962/d1004006
- Description: The prevention of outbreaks of waterborne diseases remains a major challenge to public health service providers globally. One of the major obstacles in this effort is the unavailability of on-line and real-time methods for rapid monitoring of faecal pollution to facilitate early warning of contamination of drinking water. The main objective of this study was to develop a β-glucuronidase (GUD)-based method that could be used for the on-line and real-time monitoring of microbial water quality. GUD is a marker enzyme for the faecal indicator bacteria Escherichia coli. This enzyme breaks down the synthetic substrate p-nitrophenyl-β-D-glucuronide (PNPG) to D-glucuronic acid and p-nitrophenol (PNP), which turns yellow under alkaline pH. The enzymatically produced PNP was used to detect GUD activity. In situ GUD assays were performed using running and stagnant water samples from the Bloukrans River, Grahamstown, South Africa. The physico-chemical properties of environmental GUD were determined, after which a liquid bioprobe and a microbial biosensor modified with Moraxella 1A species for the detection of the enzyme activity were developed. In order to determine the reliability and sensitivity of these methods, regression analyses for each method versus E. coli colony forming units (CFU) were performed. The storage stabilities of the bioprobe and biosensor were also investigated. The physico-chemical properties of in situ GUD were different from those of its commercially available counterpart. The temperature optimum for the former was between 35 and 40 °C while for the latter it was 45 °C. Commercial (reference) GUD had a pH optimum of 8.0 while the environmental counterpart exhibited a broad pH optimum of between pH 5.0 and 8.0. The liquid bioprobe had a limit of detection (LOD) of GUD activity equivalent to 2 CFU/100 ml and a detection time of 24 h. The method was less labour intensive and costly than the culturing method. The liquid bioprobe was stable for at least four weeks at room temperature (20 ± 2 °C). The biosensor was prepared by modifying a glassy carbon electrode with PNP degrading Moraxella 1A cells. The biosensor was 100 times more sensitive and rapid (5-20 min) than the spectrophotometric method (24 h), and was also able to detect GUD activity of viable but non-culturable cells. Thus it was more sensitive than the culturing method. Furthermore, the biosensor was selective and costeffective. The possibility of using a Pseudomonas putida JS444 biosensor was also investigated, but it was not as sensitive and selective as the Moraxella 1A biosensor. The Moraxella biosensor, therefore, offered the best option for on-line and real-time microbial water quality monitoring in South African river waters and drinking water supplies.
- Full Text:
- Date Issued: 2007
Evolutionary and biogeographic studies in the genus Kniphofia moench (Asphodelaceae)
- Authors: Ramdhani, Syd
- Date: 2007
- Subjects: Asphodelaceae Asphodelaceae -- Genetics Cladistic analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4220 , http://hdl.handle.net/10962/d1003789
- Description: Kniphofia, a genus of approximately 71 species, is almost entirely African with two species occurring in Madagascar and one in Yemen. Commonly known as ‘red hot pokers’ they are popular among horticulturists. The genus is also well known for its complex alpha taxonomy. To date, no studies have examined the phylogenetic relationships among species or the evolutionary history of the genus, and little work has been done on their biogeography. The main focus of this study was (i) to review the alpha taxonomy, (ii) to assess diversity and endemism in Kniphofia, (iii) to use DNA sequence data to reconstruct a specieslevel phylogeny to understand intra-generic species relationships and evolutionary processes (iv) to use phylogeographic approaches to study the biogeography and evaluate biogeographical patterns, and (v) to assess anatomical variation and determine if anatomical characters are useful for species delimitation. It was found that the genus has six centres of diversity, five of which are centres of endemism. The South African Centre is the most speciose and is also the largest centre of endemism. Kniphofia shows a strong Afromontane grassland affinity in Tropical and East Africa. In South Africa, it is found from high altitudes to coastal habitats, with the most speciose regions being Afromontane grasslands. It is thus not considered to be an Afromontane element, but rather an Afromontane associate. Five major evolutionary lineages were identified using cpDNA sequence data (trnT-L spacer), four of which are southern African. The fifth lineage is represented by material from Madagascar, East and Tropical Africa. The nuclear ITS region failed to provide resolution, as many sequences were identical. The five lineages recovered using cpDNA showed some congruence with geographic origin rather than the taxonomic arrangement based on morphology. All of the species with multiple samples were non-monophyletic. This could be due to hybridisation and/or incomplete lineage sorting. The nested clade analysis, although preliminary, did not completely agree with the phylogenetic analyses. One of the three third level nested clades appears to show fragmentation between the Cape Region, KwaZulu-Natal and northern parts of southern Africa. Furthermore, another nested clades recovered suggest a range expansion and radiation from the Drakensberg into the adjacent Drakensberg-Maputoland-Pondoland transition. Morphological species of Kniphofia exhibited substantial leaf anatomical variation and anatomical characters do not cluster samples into their morphological species. The anatomical results do not fit any geographic pattern, nor do they correspond to the lineages recovered using molecular markers or the nested clades. Leaf anatomical variation does not appear to be influenced by geographical or environmental factors. However, hybridisation may play a role but was not tested in this study. In light of the above findings it is proposed that the evolutionary and biogeographic history of Kniphofia is strongly linked to tectonic events, and Quaternary climatic cycles and vegetation changes. Tectonic events (viz. uplifts) may have resulted in vicariance events that may account for the five cpDNA lineages recovered in phylogenetic analyses, while Quaternary climatic cycles and vegetation changes may have had a more recent impact on evolution and biogeography. It is hypothesised that the ancestral area for Kniphofia was much more widespread when Afromontane grasslands were more extensive during cooler and drier glacial episodes. Kniphofia on the high mountains of Tropical and East Africa would have tracked Afromontane grasslands as they expanded their ranges in cooler periods. While during wetter and warmer interglacial periods Kniphofia would have retreated into refugia on the mountains of Tropical and East Africa, with no gene flow possible between these refugia. In South Africa, where latitude compensates for altitude, Kniphofia may have maintained a distribution that extended into the lowlands even during interglacials. A cyclic climate change hypothesis implies that populations of Kniphofia (at different phases of the climatic cycle) would have experienced periods of contractions and fragmentation followed by periods of range expansion and coalescence or secondary contact. Altitudinal shifting is proposed to be the most likely mechanism for fragmentation and range expansion, and would would possibly promoted hybridisation. Within the five lineages there is evidence for recent differentiation as the branch lengths are short, there are numerous nonmonophyletic species and numerous identical haplotypes (cpDNA and ITS) which collectively indicate a recent radiation in southern Africa. A recent radiation would also account for the taxonomic confusion and difficulty in differentiating morpho-species. These climatic events may also account for the substantial anatomical variation in southern African Kniphofia species.
- Full Text:
- Date Issued: 2007
- Authors: Ramdhani, Syd
- Date: 2007
- Subjects: Asphodelaceae Asphodelaceae -- Genetics Cladistic analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4220 , http://hdl.handle.net/10962/d1003789
- Description: Kniphofia, a genus of approximately 71 species, is almost entirely African with two species occurring in Madagascar and one in Yemen. Commonly known as ‘red hot pokers’ they are popular among horticulturists. The genus is also well known for its complex alpha taxonomy. To date, no studies have examined the phylogenetic relationships among species or the evolutionary history of the genus, and little work has been done on their biogeography. The main focus of this study was (i) to review the alpha taxonomy, (ii) to assess diversity and endemism in Kniphofia, (iii) to use DNA sequence data to reconstruct a specieslevel phylogeny to understand intra-generic species relationships and evolutionary processes (iv) to use phylogeographic approaches to study the biogeography and evaluate biogeographical patterns, and (v) to assess anatomical variation and determine if anatomical characters are useful for species delimitation. It was found that the genus has six centres of diversity, five of which are centres of endemism. The South African Centre is the most speciose and is also the largest centre of endemism. Kniphofia shows a strong Afromontane grassland affinity in Tropical and East Africa. In South Africa, it is found from high altitudes to coastal habitats, with the most speciose regions being Afromontane grasslands. It is thus not considered to be an Afromontane element, but rather an Afromontane associate. Five major evolutionary lineages were identified using cpDNA sequence data (trnT-L spacer), four of which are southern African. The fifth lineage is represented by material from Madagascar, East and Tropical Africa. The nuclear ITS region failed to provide resolution, as many sequences were identical. The five lineages recovered using cpDNA showed some congruence with geographic origin rather than the taxonomic arrangement based on morphology. All of the species with multiple samples were non-monophyletic. This could be due to hybridisation and/or incomplete lineage sorting. The nested clade analysis, although preliminary, did not completely agree with the phylogenetic analyses. One of the three third level nested clades appears to show fragmentation between the Cape Region, KwaZulu-Natal and northern parts of southern Africa. Furthermore, another nested clades recovered suggest a range expansion and radiation from the Drakensberg into the adjacent Drakensberg-Maputoland-Pondoland transition. Morphological species of Kniphofia exhibited substantial leaf anatomical variation and anatomical characters do not cluster samples into their morphological species. The anatomical results do not fit any geographic pattern, nor do they correspond to the lineages recovered using molecular markers or the nested clades. Leaf anatomical variation does not appear to be influenced by geographical or environmental factors. However, hybridisation may play a role but was not tested in this study. In light of the above findings it is proposed that the evolutionary and biogeographic history of Kniphofia is strongly linked to tectonic events, and Quaternary climatic cycles and vegetation changes. Tectonic events (viz. uplifts) may have resulted in vicariance events that may account for the five cpDNA lineages recovered in phylogenetic analyses, while Quaternary climatic cycles and vegetation changes may have had a more recent impact on evolution and biogeography. It is hypothesised that the ancestral area for Kniphofia was much more widespread when Afromontane grasslands were more extensive during cooler and drier glacial episodes. Kniphofia on the high mountains of Tropical and East Africa would have tracked Afromontane grasslands as they expanded their ranges in cooler periods. While during wetter and warmer interglacial periods Kniphofia would have retreated into refugia on the mountains of Tropical and East Africa, with no gene flow possible between these refugia. In South Africa, where latitude compensates for altitude, Kniphofia may have maintained a distribution that extended into the lowlands even during interglacials. A cyclic climate change hypothesis implies that populations of Kniphofia (at different phases of the climatic cycle) would have experienced periods of contractions and fragmentation followed by periods of range expansion and coalescence or secondary contact. Altitudinal shifting is proposed to be the most likely mechanism for fragmentation and range expansion, and would would possibly promoted hybridisation. Within the five lineages there is evidence for recent differentiation as the branch lengths are short, there are numerous nonmonophyletic species and numerous identical haplotypes (cpDNA and ITS) which collectively indicate a recent radiation in southern Africa. A recent radiation would also account for the taxonomic confusion and difficulty in differentiating morpho-species. These climatic events may also account for the substantial anatomical variation in southern African Kniphofia species.
- Full Text:
- Date Issued: 2007
Field and laboratory analyses of manual tasks in the South African automotive industry
- Authors: James, Jonathan Peter
- Date: 2007
- Subjects: Automobile industry and trade -- South Africa -- Safety measures , Human engineering -- South Africa , Automobile industry workers -- South Africa -- Health risk assessment , Industrial safety -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5121 , http://hdl.handle.net/10962/d1005199 , Automobile industry and trade -- South Africa -- Safety measures , Human engineering -- South Africa , Automobile industry workers -- South Africa -- Health risk assessment , Industrial safety -- South Africa
- Description: The present study adopted a “field-laboratory-field” approach in the assessment of the efficacy of ergonomics interventions specific to two selected tasks evaluated in a South African automotive industry. Initial field testing was conducted in an Eastern Cape (South Africa) automotive plant where high risk areas were identified during walkthrough ergonomics surveys in conjunction with interaction with operators. Temporal factors and working postures of 12 industrial workers were recorded and observed, while physiological and perceptual responses were assessed. Two priority areas were focused upon for analysis, namely the Paintshop and Bodyshop with the former identified as being the more taxing of the two tasks. Responses of 30 students participating in rigourously controlled laboratory simulations were subsequently collected while completing the two tasks, namely the Paintshop Trolley Transfer (PTT) and Car Door Carriage (CDC) for participants. Working postures, kinematic, physiological and perceptual responses were assessed pre- and post-intervention. Following the laboratory experimentation a basic re-evaluation was conducted at the plant to assess whether the proposed changes had a positive effect on working postures, physiological and perceptual responses. The results of the preliminary field investigation revealed a prevalence of awkward working postures and excessive manual work in both areas. Laboratory experimentation revealed a notable reduction in task demands pre- versus post-intervention. The PTT mean lean angle for two-handed pre-intervention pulling observations of 23.7° (±3.51) was reduced to 13.9° (±2.21) post-intervention. Low back disorder (LBD) risk was reduced during the two-handed pull intervention (from 36.8% ±8.03 to 21.7% ±5.31). A significant decrement in heart rate responses from 103 bt.min-1 (±11.62) to 93 bt.min[superscript -1] (±11.77) was recorded during the two-handed symmetrical pushing intervention. The electromyography (EMG) responses for one-handed pushing and pulling pre-intervention showed the highest levels of muscular activity in the right medial deltoid due to an awkward and asymmetrical posture. CDC responses demonstrated that minor changes in the storage height of the door resulted in a significant reduction in sagittal flexion from 28.0° (±4.78) to 20.7° (±5.65). Predictions of average probability of LBD risk were significantly reduced from 50.3% (±5.91) to 39.8% (±5.10) for post-intervention car door lifting. In addition, the greatest reduction in EMG activity as a %MVC was achieved during sub-task ii (reduced from 35.1 to 13.7% and 30.5 to 13.9% for left and right erector spinae respectively) which was associated with the introduction of the transfer trolley for the door transfer phase of the CDC. Re-evaluation in the automotive plant revealed that the most notable change has been the implementation of automated ride on trolleys in the Paintshop. The Bodyshop area has also been modified to allow more effective job rotation and the step into the storage bin has been reduced via a “low-cost” stepping platform. Mean heart rate recordings were reduced from 94 (±9.77) bt.min[superscript -1] to 81 (±3.72) bt.min[superscript -1] in the Paintshop. Overall the results demonstrate the effect of “low-cost” interventions in reducing the physical stresses placed on workers in the automotive industry where much of the work is still done manually.
- Full Text:
- Date Issued: 2007
- Authors: James, Jonathan Peter
- Date: 2007
- Subjects: Automobile industry and trade -- South Africa -- Safety measures , Human engineering -- South Africa , Automobile industry workers -- South Africa -- Health risk assessment , Industrial safety -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5121 , http://hdl.handle.net/10962/d1005199 , Automobile industry and trade -- South Africa -- Safety measures , Human engineering -- South Africa , Automobile industry workers -- South Africa -- Health risk assessment , Industrial safety -- South Africa
- Description: The present study adopted a “field-laboratory-field” approach in the assessment of the efficacy of ergonomics interventions specific to two selected tasks evaluated in a South African automotive industry. Initial field testing was conducted in an Eastern Cape (South Africa) automotive plant where high risk areas were identified during walkthrough ergonomics surveys in conjunction with interaction with operators. Temporal factors and working postures of 12 industrial workers were recorded and observed, while physiological and perceptual responses were assessed. Two priority areas were focused upon for analysis, namely the Paintshop and Bodyshop with the former identified as being the more taxing of the two tasks. Responses of 30 students participating in rigourously controlled laboratory simulations were subsequently collected while completing the two tasks, namely the Paintshop Trolley Transfer (PTT) and Car Door Carriage (CDC) for participants. Working postures, kinematic, physiological and perceptual responses were assessed pre- and post-intervention. Following the laboratory experimentation a basic re-evaluation was conducted at the plant to assess whether the proposed changes had a positive effect on working postures, physiological and perceptual responses. The results of the preliminary field investigation revealed a prevalence of awkward working postures and excessive manual work in both areas. Laboratory experimentation revealed a notable reduction in task demands pre- versus post-intervention. The PTT mean lean angle for two-handed pre-intervention pulling observations of 23.7° (±3.51) was reduced to 13.9° (±2.21) post-intervention. Low back disorder (LBD) risk was reduced during the two-handed pull intervention (from 36.8% ±8.03 to 21.7% ±5.31). A significant decrement in heart rate responses from 103 bt.min-1 (±11.62) to 93 bt.min[superscript -1] (±11.77) was recorded during the two-handed symmetrical pushing intervention. The electromyography (EMG) responses for one-handed pushing and pulling pre-intervention showed the highest levels of muscular activity in the right medial deltoid due to an awkward and asymmetrical posture. CDC responses demonstrated that minor changes in the storage height of the door resulted in a significant reduction in sagittal flexion from 28.0° (±4.78) to 20.7° (±5.65). Predictions of average probability of LBD risk were significantly reduced from 50.3% (±5.91) to 39.8% (±5.10) for post-intervention car door lifting. In addition, the greatest reduction in EMG activity as a %MVC was achieved during sub-task ii (reduced from 35.1 to 13.7% and 30.5 to 13.9% for left and right erector spinae respectively) which was associated with the introduction of the transfer trolley for the door transfer phase of the CDC. Re-evaluation in the automotive plant revealed that the most notable change has been the implementation of automated ride on trolleys in the Paintshop. The Bodyshop area has also been modified to allow more effective job rotation and the step into the storage bin has been reduced via a “low-cost” stepping platform. Mean heart rate recordings were reduced from 94 (±9.77) bt.min[superscript -1] to 81 (±3.72) bt.min[superscript -1] in the Paintshop. Overall the results demonstrate the effect of “low-cost” interventions in reducing the physical stresses placed on workers in the automotive industry where much of the work is still done manually.
- Full Text:
- Date Issued: 2007
Genesis of environmental education policy in Botswana: construction and interpretation
- Ketlhoilwe, Mphemelang Joseph
- Authors: Ketlhoilwe, Mphemelang Joseph
- Date: 2007
- Subjects: Environmental education -- Botswana Education and state -- Botswana
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1549 , http://hdl.handle.net/10962/d1003431
- Description: This study is based on the 1994 Revised National Policy on Education (Botswana Government, 1994) that introduced environmental education into the Botswana’s education system. The main goals of this study were to understand the genealogy of and to critically analyze governmentality associated with environmental education policy in Botswana. Drawing on a post-structural genealogical approach to the subject matter (following Foucault) global historical events and their influence on policy in Botswana, views on environmental education and interpretation, and power relations in environmental education policy discourses were investigated. An investigation was conducted through document analysis, interviews, focus group discussions and observations. The analysis revealed that power relations have historically transcended environmental education policy discourses from global, regional to national levels. The exercise of power through international bodies, and bilateral and multilateral agreements has impacted on Botswana enabling her to enact policies to address socio-ecological crises or regulating them to sustainably utilize natural resources. However, evidence has shown that although Botswana accepted and introduced environmental education, structures were not ready for its implementation and hence some contextual problems are experienced by teachers in schools. The Revised National Policy on Education (RNPE) was constructed through a consultative process, but the final decision on what goes into the policy text was decided from the top (i.e. by the Ministry of Education senior officials). It emerged from this study that Botswana has inconsistently adopted sustainability and conservation-protection discourses in environmental education policy. The mix of the two discourses shows continuity of the protectionist-conservationist discourses and emergence of the current sustainable use discourse, creating a complex discourse environment. The study also revealed that in including these primarily western scientific discourses, other discourses were marginalized or excluded, which revealed continuity with colonial education discourses. The findings also revealed variance in the understanding of environmental education. The majority of the teachers understood and normalised new knowledge in environmental education as Environmental Science or Science, and equated environmental management activities with environmental education. Teachers deployed new governmentalities and normalizing strategies by following the traditional conservation and science epistemological and pedagogical discourses. They exercised various self-governing strategies to respond to the RNPE requirement regarding environmental education. The findings highlight the need for re-conceptualization of environmental education at macro(at Ministry of Education) and micro level. There is a need to harmonize the variation in policy interpretations and clarification of the conservation/environmental education and sustainability discourses running parallel in schools or to work more explicitly with multiple discourses. It has also emerged that teacher support mechanisms need review to enhance policy implementation. The study recommends that further and explicit analysis of environmental education discourses is critical for shaping the future of environmental education policy development and interpretation within Botswana’s education system.
- Full Text:
- Date Issued: 2007
- Authors: Ketlhoilwe, Mphemelang Joseph
- Date: 2007
- Subjects: Environmental education -- Botswana Education and state -- Botswana
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1549 , http://hdl.handle.net/10962/d1003431
- Description: This study is based on the 1994 Revised National Policy on Education (Botswana Government, 1994) that introduced environmental education into the Botswana’s education system. The main goals of this study were to understand the genealogy of and to critically analyze governmentality associated with environmental education policy in Botswana. Drawing on a post-structural genealogical approach to the subject matter (following Foucault) global historical events and their influence on policy in Botswana, views on environmental education and interpretation, and power relations in environmental education policy discourses were investigated. An investigation was conducted through document analysis, interviews, focus group discussions and observations. The analysis revealed that power relations have historically transcended environmental education policy discourses from global, regional to national levels. The exercise of power through international bodies, and bilateral and multilateral agreements has impacted on Botswana enabling her to enact policies to address socio-ecological crises or regulating them to sustainably utilize natural resources. However, evidence has shown that although Botswana accepted and introduced environmental education, structures were not ready for its implementation and hence some contextual problems are experienced by teachers in schools. The Revised National Policy on Education (RNPE) was constructed through a consultative process, but the final decision on what goes into the policy text was decided from the top (i.e. by the Ministry of Education senior officials). It emerged from this study that Botswana has inconsistently adopted sustainability and conservation-protection discourses in environmental education policy. The mix of the two discourses shows continuity of the protectionist-conservationist discourses and emergence of the current sustainable use discourse, creating a complex discourse environment. The study also revealed that in including these primarily western scientific discourses, other discourses were marginalized or excluded, which revealed continuity with colonial education discourses. The findings also revealed variance in the understanding of environmental education. The majority of the teachers understood and normalised new knowledge in environmental education as Environmental Science or Science, and equated environmental management activities with environmental education. Teachers deployed new governmentalities and normalizing strategies by following the traditional conservation and science epistemological and pedagogical discourses. They exercised various self-governing strategies to respond to the RNPE requirement regarding environmental education. The findings highlight the need for re-conceptualization of environmental education at macro(at Ministry of Education) and micro level. There is a need to harmonize the variation in policy interpretations and clarification of the conservation/environmental education and sustainability discourses running parallel in schools or to work more explicitly with multiple discourses. It has also emerged that teacher support mechanisms need review to enhance policy implementation. The study recommends that further and explicit analysis of environmental education discourses is critical for shaping the future of environmental education policy development and interpretation within Botswana’s education system.
- Full Text:
- Date Issued: 2007
Hermeneutic and empirical analyses of graphically inspired metamathematics that reflect critical consciousness within perspectives of personal and social justice
- Authors: Van Jaarsveld, Pieter Paul
- Date: 2007
- Subjects: Mathematics -- Study and teaching -- South Africa Mathematics -- Study and teaching -- Social aspects -- South Africa Algorithms -- Study and teaching -- South Africa Critical pedagogy Metacognition Hermeneutics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1842 , http://hdl.handle.net/10962/d1004376
- Description: My involvement with mathematics education amongst township educators and learners over the past seven years has highlighted the absence of sustained meaning and meaning making of mathematical concepts. It appears though that this instrumental rather than relational understanding of FET mathematics is not unique to township learners but is encountered amongst learners of all socio-economic classes and is representative of many FET mathematics learners. Given that the language of learning and teaching is a major contributory factor in the South African education system, it appears that the language of mathematics itself is a greater exacerbating factor for many learners of mathematics. The exclusive algorithmic approach to classroom mathematics further seems to alienate many learners from the essence of the meaning of mathematical tasks. This research undertakes to determine whether metateaching and metalearning as forerunners to metacognition facilitates the acquisition of the sustained meaning of mathematical concepts. Metateaching and metalearning refer to the acute and deliberate awareness by educator and learner as to what constitutes concepts. Teaching and learning therefore presupposes the deconstruction of concepts into its subsumed derivative roots. It also assumes an awareness of the tacit degrees of abstraction that characterise tasks and the content of tasks. This in turn has implications for the educator's adopted sequence of topics for instruction. Metacognition implies awareness on the part of the learner (and educator) as to how material is learned and a further awareness as to how that learning can be sustained. Whether we ascribe meaningful learning to radical or social constructivism, or to associationist didactive approaches, or a combination of these, we are making assumptions about how learners acquire and sustain mathematical meaning because mathematics is, by and large a symbolic language often devoid of affective connotation. Furthermore our assessments of learners' tasks amount to clinical corrections of austere formulae wrapped in algorithmic procedures which manifest nothing of a learner's experience of mathematics or the deeper understanding (or misunderstandings) which characterise a learning and/or assessment episode. To this end the research design of this interpretive case study requires learners to expound in textual accounts their thoughts as they describe the evolution of a mathematical process as they approach a solution and eventually interpret it. The textual account exposes the concept definition for what it really is in a learner's understanding of it and it is the expressiveness of language that indicates whether the understanding of a learner is approaching the concept image. The textual accounts vary in richness in terms of mathematical register and this in turn reflects the conceptual depth. The mechanism which seems to promote the conversion from concept definition to concept image is the graphical representation of the mathematical task or procedure, possibly because of its greater concreteness as opposed to the abstraction of its algebraic form.
- Full Text:
- Date Issued: 2007
- Authors: Van Jaarsveld, Pieter Paul
- Date: 2007
- Subjects: Mathematics -- Study and teaching -- South Africa Mathematics -- Study and teaching -- Social aspects -- South Africa Algorithms -- Study and teaching -- South Africa Critical pedagogy Metacognition Hermeneutics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1842 , http://hdl.handle.net/10962/d1004376
- Description: My involvement with mathematics education amongst township educators and learners over the past seven years has highlighted the absence of sustained meaning and meaning making of mathematical concepts. It appears though that this instrumental rather than relational understanding of FET mathematics is not unique to township learners but is encountered amongst learners of all socio-economic classes and is representative of many FET mathematics learners. Given that the language of learning and teaching is a major contributory factor in the South African education system, it appears that the language of mathematics itself is a greater exacerbating factor for many learners of mathematics. The exclusive algorithmic approach to classroom mathematics further seems to alienate many learners from the essence of the meaning of mathematical tasks. This research undertakes to determine whether metateaching and metalearning as forerunners to metacognition facilitates the acquisition of the sustained meaning of mathematical concepts. Metateaching and metalearning refer to the acute and deliberate awareness by educator and learner as to what constitutes concepts. Teaching and learning therefore presupposes the deconstruction of concepts into its subsumed derivative roots. It also assumes an awareness of the tacit degrees of abstraction that characterise tasks and the content of tasks. This in turn has implications for the educator's adopted sequence of topics for instruction. Metacognition implies awareness on the part of the learner (and educator) as to how material is learned and a further awareness as to how that learning can be sustained. Whether we ascribe meaningful learning to radical or social constructivism, or to associationist didactive approaches, or a combination of these, we are making assumptions about how learners acquire and sustain mathematical meaning because mathematics is, by and large a symbolic language often devoid of affective connotation. Furthermore our assessments of learners' tasks amount to clinical corrections of austere formulae wrapped in algorithmic procedures which manifest nothing of a learner's experience of mathematics or the deeper understanding (or misunderstandings) which characterise a learning and/or assessment episode. To this end the research design of this interpretive case study requires learners to expound in textual accounts their thoughts as they describe the evolution of a mathematical process as they approach a solution and eventually interpret it. The textual account exposes the concept definition for what it really is in a learner's understanding of it and it is the expressiveness of language that indicates whether the understanding of a learner is approaching the concept image. The textual accounts vary in richness in terms of mathematical register and this in turn reflects the conceptual depth. The mechanism which seems to promote the conversion from concept definition to concept image is the graphical representation of the mathematical task or procedure, possibly because of its greater concreteness as opposed to the abstraction of its algebraic form.
- Full Text:
- Date Issued: 2007
Isolation and characterization of genes encoding heat shock protein 70s (hsp 70s) from two species of the coelacanth, Latimeria chalumnae and Latimeria menadoensis
- Modisakeng, Keoagile William
- Authors: Modisakeng, Keoagile William
- Date: 2007
- Subjects: Coelacanth Coelacanth -- Genetics Heat shock proteins Molecular chaperones Proteins -- Analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3971 , http://hdl.handle.net/10962/d1004030
- Description: The extant coelacanths have a close resemblance to the coelacanth fossil records dating back to 230mya. Like their predecessors, the extant coelacanths inhabit rocky caves at a depth of 100-300m below sea level. In the Comoros, the water temperature at these depths is estimated to fluctuate between 14-20°C. High-level adaptation to these environment and lack of competition are thought to have led to the morphological uniformity and slow change throughout the history of the coelacanths. Under stress conditions, proteins unfold or misfold leading to the formation of aggregates. Molecular chaperones facilitate the correct folding of other proteins so that they can attain a stable tertiary structure. In addition, molecular chaperones aid the refolding of denatured proteins and the degradation of terminally misfolded protein after cellular stress. Heat shock proteins form one of the major classes of molecular chaperones. Here we show that, despite high-level adaptation to a unique habitat and slow change, the genome of the coelacanth encodes the major and highly conserved molecular chaperone, Hsp70. Latimeria menadoensis and Latimeria chalumnae contain intronless hsp70 genes encoding Hsp70 proteins archetypal of known Hsp70s. Based on the coelacanth codon usage, we have shown that bacterial protein expression systems, particularly Escherichia coli, may not be appropriate for the overproduction of coelacanth Hsp70s and coelacanth proteins in general. Also interesting, was the discovery that like the rat Hsc70, the L. menadoensis Hsp70 could not reverse thermal sensitivity in a temperate sensitive E. coli DnaK mutant strain, BB2362. We also report the successful isolation of a 1.2 kb region of L. menadoensis hsp70 upstream regulatory region. This region contain three putative heat shock elements, a TATA- box and two CAAT-boxes. This regulatory region resembled the Xenopus, mouse, and particularly tilapia hsp70 promoters, all of which have been shown to drive the expression of reporter genes in a heat dependent manner. Taken together, this data is the first to strongly suggest an inducible Hsp70-base cytoprotection mechanism in the coelacanth. It further provides basis to formulate testable predictions about the regulation, structure and function of Hsp70s in the living fossil, Latimeria.
- Full Text:
- Date Issued: 2007
- Authors: Modisakeng, Keoagile William
- Date: 2007
- Subjects: Coelacanth Coelacanth -- Genetics Heat shock proteins Molecular chaperones Proteins -- Analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3971 , http://hdl.handle.net/10962/d1004030
- Description: The extant coelacanths have a close resemblance to the coelacanth fossil records dating back to 230mya. Like their predecessors, the extant coelacanths inhabit rocky caves at a depth of 100-300m below sea level. In the Comoros, the water temperature at these depths is estimated to fluctuate between 14-20°C. High-level adaptation to these environment and lack of competition are thought to have led to the morphological uniformity and slow change throughout the history of the coelacanths. Under stress conditions, proteins unfold or misfold leading to the formation of aggregates. Molecular chaperones facilitate the correct folding of other proteins so that they can attain a stable tertiary structure. In addition, molecular chaperones aid the refolding of denatured proteins and the degradation of terminally misfolded protein after cellular stress. Heat shock proteins form one of the major classes of molecular chaperones. Here we show that, despite high-level adaptation to a unique habitat and slow change, the genome of the coelacanth encodes the major and highly conserved molecular chaperone, Hsp70. Latimeria menadoensis and Latimeria chalumnae contain intronless hsp70 genes encoding Hsp70 proteins archetypal of known Hsp70s. Based on the coelacanth codon usage, we have shown that bacterial protein expression systems, particularly Escherichia coli, may not be appropriate for the overproduction of coelacanth Hsp70s and coelacanth proteins in general. Also interesting, was the discovery that like the rat Hsc70, the L. menadoensis Hsp70 could not reverse thermal sensitivity in a temperate sensitive E. coli DnaK mutant strain, BB2362. We also report the successful isolation of a 1.2 kb region of L. menadoensis hsp70 upstream regulatory region. This region contain three putative heat shock elements, a TATA- box and two CAAT-boxes. This regulatory region resembled the Xenopus, mouse, and particularly tilapia hsp70 promoters, all of which have been shown to drive the expression of reporter genes in a heat dependent manner. Taken together, this data is the first to strongly suggest an inducible Hsp70-base cytoprotection mechanism in the coelacanth. It further provides basis to formulate testable predictions about the regulation, structure and function of Hsp70s in the living fossil, Latimeria.
- Full Text:
- Date Issued: 2007
Management of invasive aquatic weeds with emphasis on biological control in Senegal
- Authors: Diop, Ousseynou
- Date: 2007
- Subjects: Aquatic weeds -- Biological control -- Senegal Invasive plants -- Biological control -- Senegal Aquatic plants -- Biological control -- Senegal
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5728 , http://hdl.handle.net/10962/d1005414
- Description: In 1985 the Diama Dam was built near the mouth of the Senegal River to regulate flows during the rainy season and prevent the intrusion of seawater during the dry season. This created ideal conditions upstream of the dam wall for invasion by two highly invasive aquatic weeds, first by water lettuce Pistia stratiotes Linnaeus (Araceae) in 1993, and then by salvinia Salvinia molesta D.S. Mitchell (Salviniaceae) in 1999. This study was focused on the management of P. stratiotes and S. molesta. Following successes that were achieved elsewhere in the world, biological control programmes involving two weevil species were inaugurated against both weeds and research was focused on several aspects. These included pre-release studies to determine the weevils' host-specificity and impact on the plants in the laboratory, their subsequent mass-rearing and releases at selected sites and post-release evaluations on their impact on the weed populations in the field. Both programmes, which reprepresented the first biocontrol efforts against aquatic weeds in Senegal, proved highly successful with severe damage inflicted on the weed populations and complete control achieved within a relatively short time span. A laboratory exclusion experiment with N. affinis on P. stratiotes showed that in treated tubs, the weevil strongly depressed plant performance as measured by the plant growth parameters: mass, rosette diameter, root length, number of leaves and daughter plants whereas control plants were healthy. Field releases started in September 1994 and water coverage by P. stratiotes at Lake Guiers was reduced by 25% in January 1995 and 50% in April 1995. A general decline of 65% in water coverage by P. stratiotes was observed in June 1995 and by August 1995, eight months after releases P. stratiotes mats were destroyed. Further, although no releases were made there, good results were obtained within 18 months at Djoudj Park water bodies, located 150 km NW from Lake Guiers indicating the potential of the weevil to disperse long distances. In 2005, P. stratiotes reappeared and the weevil N. affinis has located and controlled all of these P. stratiotes recurrences after new releases. In 1999, S. molesta covered an estimated area of 18 000 ha on the Senegal River Left Bank and tributaries (Senegal) and 7 840 ha on the Senegal River Right Bank (Mauritania). Military and Civil Development Committee (CCMAD) and community volunteers made an effort to control S. molesta using physical removal, but this costly and labour-intensive approach was unsustainable. Hence, biological control was adopted by Senegal and Mauritania to manage the weed. Host range tests to assess feeding by C. salviniae on S. molesta and non-target plants and carried out on 13 crop species showed that no feeding damage was observed on the latter and weevils only fed on S. molesta. Field releases of some 48 953 weevils at 270 sites were made from early January 2002 to August 2002. Within one year, weevils were established and were being recovered up to 50 km from the release sites. In a case study conducted at one of the release sites, the S. molesta infestation was reduced from 100% to less than 3% 24 months after release. These results are discussed in the context of the weeds’ negative impact on aquatic systems and riverside communities, and in the involvement of these communities in the programmes.
- Full Text:
- Date Issued: 2007
- Authors: Diop, Ousseynou
- Date: 2007
- Subjects: Aquatic weeds -- Biological control -- Senegal Invasive plants -- Biological control -- Senegal Aquatic plants -- Biological control -- Senegal
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5728 , http://hdl.handle.net/10962/d1005414
- Description: In 1985 the Diama Dam was built near the mouth of the Senegal River to regulate flows during the rainy season and prevent the intrusion of seawater during the dry season. This created ideal conditions upstream of the dam wall for invasion by two highly invasive aquatic weeds, first by water lettuce Pistia stratiotes Linnaeus (Araceae) in 1993, and then by salvinia Salvinia molesta D.S. Mitchell (Salviniaceae) in 1999. This study was focused on the management of P. stratiotes and S. molesta. Following successes that were achieved elsewhere in the world, biological control programmes involving two weevil species were inaugurated against both weeds and research was focused on several aspects. These included pre-release studies to determine the weevils' host-specificity and impact on the plants in the laboratory, their subsequent mass-rearing and releases at selected sites and post-release evaluations on their impact on the weed populations in the field. Both programmes, which reprepresented the first biocontrol efforts against aquatic weeds in Senegal, proved highly successful with severe damage inflicted on the weed populations and complete control achieved within a relatively short time span. A laboratory exclusion experiment with N. affinis on P. stratiotes showed that in treated tubs, the weevil strongly depressed plant performance as measured by the plant growth parameters: mass, rosette diameter, root length, number of leaves and daughter plants whereas control plants were healthy. Field releases started in September 1994 and water coverage by P. stratiotes at Lake Guiers was reduced by 25% in January 1995 and 50% in April 1995. A general decline of 65% in water coverage by P. stratiotes was observed in June 1995 and by August 1995, eight months after releases P. stratiotes mats were destroyed. Further, although no releases were made there, good results were obtained within 18 months at Djoudj Park water bodies, located 150 km NW from Lake Guiers indicating the potential of the weevil to disperse long distances. In 2005, P. stratiotes reappeared and the weevil N. affinis has located and controlled all of these P. stratiotes recurrences after new releases. In 1999, S. molesta covered an estimated area of 18 000 ha on the Senegal River Left Bank and tributaries (Senegal) and 7 840 ha on the Senegal River Right Bank (Mauritania). Military and Civil Development Committee (CCMAD) and community volunteers made an effort to control S. molesta using physical removal, but this costly and labour-intensive approach was unsustainable. Hence, biological control was adopted by Senegal and Mauritania to manage the weed. Host range tests to assess feeding by C. salviniae on S. molesta and non-target plants and carried out on 13 crop species showed that no feeding damage was observed on the latter and weevils only fed on S. molesta. Field releases of some 48 953 weevils at 270 sites were made from early January 2002 to August 2002. Within one year, weevils were established and were being recovered up to 50 km from the release sites. In a case study conducted at one of the release sites, the S. molesta infestation was reduced from 100% to less than 3% 24 months after release. These results are discussed in the context of the weeds’ negative impact on aquatic systems and riverside communities, and in the involvement of these communities in the programmes.
- Full Text:
- Date Issued: 2007
Molecular characterisation of the chaperone properties of Plasmodium falciparum heat shock protein 70
- Authors: Shonhai, Addmore
- Date: 2007
- Subjects: Heat shock proteins Plasmodium falciparum Protein folding Proteins -- Purification Molecular chaperones Malaria -- Prevention
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3977 , http://hdl.handle.net/10962/d1004036
- Description: Heat shock protein 70 (called DnaK in prokaryotes) is one of the most prominent groups of chaperones whose role is to prevent and reverse protein misfolding. PfHsp70 is a heatinducible cytoplasm/nuclear localised Plasmodium falciparum Hsp70. PfHsp70 is thought to confer chaperone cytoprotection to P. falciparum during the development of malaria fever. The objective of this study was to examine the chaperone properties of PfHsp70 using a bioinformatics approach, coupled to in vivo and in vitro analysis. Structural motifs that qualify PfHsp70 as a typical Hsp70 chaperone were identified. Although PfHsp70 has a higher similarity to human Hsc70 than E. coli DnaK, in vivocomplementation assays showed that PfHsp70 was able to reverse the thermosensitivity of E. coli dnaK756 (a temperature sensitive strain whose DnaK is functionally compromised). Two residues (V401 and Q402) in the linker region of PfHsp70 that are critical for its in vivo function were identified. Constructs were generated that encoded the ATPase domain of PfHsp70 and the peptide binding domain of E. coli DnaK (to generate PfK chimera); and the ATPase domain of E. coli DnaK fused to the peptide binding domain of PfHsp70 (KPf). The two chimeras were tested for their ability to reverse the thermosensitivity of E. coli dnaK756 cells. Whilst KPf was able to reverse the thermosensitivity of the E. coli dnaK756 cells, PfK could not. Previously, PfHsp70 purification involved urea denaturation. Using a detergent, polyethylenimine (PEI), PfHsp70 was natively purified. Natively purified PfHsp70 had a basal ATPase activity approximately two times higher than the previously reported activity for the protein purified through urea denaturation. PfJ4, a type II Hsp40, could not stimulate the ATPase activity of PfHsp70 in vitro. Arch and hydrophobic pocket substitutions (A419Y, Y444A and V451F) were introduced in the PfHsp70 peptide binding domain. Similar substitutions were also introduced in the KPf chimera. PfHsp70-V451F (hydrophobic pocket mutant) had marginally compromised in vivo function. However, a similar mutation (V436F), introduced in KPf abrogated the in vivo function of this chimera. The arch and hydrophobic pocket derivatives of PfHsp70 exhibited marginally compromised in vivo function, whilst equivalent mutations in KPf did not affect its in vivo function. The ability of PfHsp70 and its arch/hydrophobic pocket mutants to suppress the heatinduced aggregation of malate dehydrogenase (MDH) in vitro was investigated. Whilst PfHsp70 arch mutants displayed marginal functional loss in vivo, data from in vitro studies revealed that their functional deficiencies were more severe. This is the first study in which an Hsp70 from a parasitic eukaryote was able to suppress the thermosensitivity of an E. coli DnaK mutant strain. Findings from the in vivo and in vitro assays conducted on PfHsp70 suggest that this protein plays a key role in the life-cycle of P. falciparum. Furthermore, this study raised insights that are pertinent to the current dogma on the Hsp70 mechanism of action.
- Full Text:
- Date Issued: 2007
- Authors: Shonhai, Addmore
- Date: 2007
- Subjects: Heat shock proteins Plasmodium falciparum Protein folding Proteins -- Purification Molecular chaperones Malaria -- Prevention
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3977 , http://hdl.handle.net/10962/d1004036
- Description: Heat shock protein 70 (called DnaK in prokaryotes) is one of the most prominent groups of chaperones whose role is to prevent and reverse protein misfolding. PfHsp70 is a heatinducible cytoplasm/nuclear localised Plasmodium falciparum Hsp70. PfHsp70 is thought to confer chaperone cytoprotection to P. falciparum during the development of malaria fever. The objective of this study was to examine the chaperone properties of PfHsp70 using a bioinformatics approach, coupled to in vivo and in vitro analysis. Structural motifs that qualify PfHsp70 as a typical Hsp70 chaperone were identified. Although PfHsp70 has a higher similarity to human Hsc70 than E. coli DnaK, in vivocomplementation assays showed that PfHsp70 was able to reverse the thermosensitivity of E. coli dnaK756 (a temperature sensitive strain whose DnaK is functionally compromised). Two residues (V401 and Q402) in the linker region of PfHsp70 that are critical for its in vivo function were identified. Constructs were generated that encoded the ATPase domain of PfHsp70 and the peptide binding domain of E. coli DnaK (to generate PfK chimera); and the ATPase domain of E. coli DnaK fused to the peptide binding domain of PfHsp70 (KPf). The two chimeras were tested for their ability to reverse the thermosensitivity of E. coli dnaK756 cells. Whilst KPf was able to reverse the thermosensitivity of the E. coli dnaK756 cells, PfK could not. Previously, PfHsp70 purification involved urea denaturation. Using a detergent, polyethylenimine (PEI), PfHsp70 was natively purified. Natively purified PfHsp70 had a basal ATPase activity approximately two times higher than the previously reported activity for the protein purified through urea denaturation. PfJ4, a type II Hsp40, could not stimulate the ATPase activity of PfHsp70 in vitro. Arch and hydrophobic pocket substitutions (A419Y, Y444A and V451F) were introduced in the PfHsp70 peptide binding domain. Similar substitutions were also introduced in the KPf chimera. PfHsp70-V451F (hydrophobic pocket mutant) had marginally compromised in vivo function. However, a similar mutation (V436F), introduced in KPf abrogated the in vivo function of this chimera. The arch and hydrophobic pocket derivatives of PfHsp70 exhibited marginally compromised in vivo function, whilst equivalent mutations in KPf did not affect its in vivo function. The ability of PfHsp70 and its arch/hydrophobic pocket mutants to suppress the heatinduced aggregation of malate dehydrogenase (MDH) in vitro was investigated. Whilst PfHsp70 arch mutants displayed marginal functional loss in vivo, data from in vitro studies revealed that their functional deficiencies were more severe. This is the first study in which an Hsp70 from a parasitic eukaryote was able to suppress the thermosensitivity of an E. coli DnaK mutant strain. Findings from the in vivo and in vitro assays conducted on PfHsp70 suggest that this protein plays a key role in the life-cycle of P. falciparum. Furthermore, this study raised insights that are pertinent to the current dogma on the Hsp70 mechanism of action.
- Full Text:
- Date Issued: 2007
Networking: enabling professional development and institutionalisation of environmental education courses in Southern Africa
- Authors: Lupele, Justin Kalaba
- Date: 2007
- Subjects: SADC Regional Environmental Education Programme Education -- Africa, Southern Environmental education -- Africa, Southern Environmental education -- Study and teaching -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1678 , http://hdl.handle.net/10962/d1003561
- Description: This study was aimed at understanding how networking can enable or constrain professional development and institutionalisation of environmental education courses in southern Africa in the context of the Course Development Network (CDN), a project of the Southern Africa Development Community Regional Environmental Education Programme (SADC REEP). The study involved 12 institutions (each institution was represented in the CDN by a staff member) in eight SADC member states. It was contextualized through a review of social, political, environmental and educational developments in southern Africa, with specific reference to regionalization processes, as the SADC REEF is constituted under this post-colonial political framework. Relational philosophy informed the research process and methodology. The philosophy underpinned three distinct, yet related theoretical perspectives namely: critical realism, which provided the ontological perspectives of the study; Actor Network Theory; and Community of Practice, which provided the epistemological perspectives. Data was generated during a 33-month period in southern Africa and 12 months in the United Kingdom (and on a one week visit to Italy). During a 12 month Split Site Commonwealth Scholarship award, tenable at Manchester Metropolitan University in the United Kingdom, I studied the Environment and School Initiative (ENSI) and the Sustainability Education for European Primary Schools (SEEPS), to examine whether the same mechanisms that made them successful would apply to the CDN in the context of southern Africa. While these two case studies provided useful insight into the relationship between networking, professional development and institutionalization of environmental education programmes, they were not the main focus of the research. Data analysis was mainly through inductive, abductive and retroductive modes of inference. Inductive data analysis was done by means of Nvivo – a computer software package used for qualitative data analysis. The software aided in revealing features and relationships in the data in more depth as it allowed flexibility in working with data. Abduction is the interpretation of a phenomenon by means of a conceptual framework. In this study, I used Actor Network Theory (ANT) (Latour & Woolgar, 1979; Callon, 1986) and Community of Practice (COP) (Lave, 1988; Lave & Wenger, 1991; Fullan, 2003) as analytical conceptual frameworks to probe networking and professional development respectively. Data on institutionalisation of environmental education courses was analysed by means of retroductive mode of inference, which is a thought operation that enables the understanding of social reality beyond what is empirically observable or experienced. This study reveals that there were a number of necessary (internal) and contingent (external) factors that enabled or constrained networking, professional development and institutionalisation of environmental education courses in the context of the CDN. The key factors included existing cultural capital, donor political economy, power relations, poverty related factors and social transformation trends. The study found that relational approaches and the use of three relational theoretical lenses provided a broader lens which enabled this study to identify different dynamics, greater ontological depth and understanding of the relational dynamics and relations at play in the CDN beyond the participants' experience and observable events. The study also contends that networking can provide a support structure for social transformation and change in environmental education.
- Full Text:
- Date Issued: 2007
- Authors: Lupele, Justin Kalaba
- Date: 2007
- Subjects: SADC Regional Environmental Education Programme Education -- Africa, Southern Environmental education -- Africa, Southern Environmental education -- Study and teaching -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1678 , http://hdl.handle.net/10962/d1003561
- Description: This study was aimed at understanding how networking can enable or constrain professional development and institutionalisation of environmental education courses in southern Africa in the context of the Course Development Network (CDN), a project of the Southern Africa Development Community Regional Environmental Education Programme (SADC REEP). The study involved 12 institutions (each institution was represented in the CDN by a staff member) in eight SADC member states. It was contextualized through a review of social, political, environmental and educational developments in southern Africa, with specific reference to regionalization processes, as the SADC REEF is constituted under this post-colonial political framework. Relational philosophy informed the research process and methodology. The philosophy underpinned three distinct, yet related theoretical perspectives namely: critical realism, which provided the ontological perspectives of the study; Actor Network Theory; and Community of Practice, which provided the epistemological perspectives. Data was generated during a 33-month period in southern Africa and 12 months in the United Kingdom (and on a one week visit to Italy). During a 12 month Split Site Commonwealth Scholarship award, tenable at Manchester Metropolitan University in the United Kingdom, I studied the Environment and School Initiative (ENSI) and the Sustainability Education for European Primary Schools (SEEPS), to examine whether the same mechanisms that made them successful would apply to the CDN in the context of southern Africa. While these two case studies provided useful insight into the relationship between networking, professional development and institutionalization of environmental education programmes, they were not the main focus of the research. Data analysis was mainly through inductive, abductive and retroductive modes of inference. Inductive data analysis was done by means of Nvivo – a computer software package used for qualitative data analysis. The software aided in revealing features and relationships in the data in more depth as it allowed flexibility in working with data. Abduction is the interpretation of a phenomenon by means of a conceptual framework. In this study, I used Actor Network Theory (ANT) (Latour & Woolgar, 1979; Callon, 1986) and Community of Practice (COP) (Lave, 1988; Lave & Wenger, 1991; Fullan, 2003) as analytical conceptual frameworks to probe networking and professional development respectively. Data on institutionalisation of environmental education courses was analysed by means of retroductive mode of inference, which is a thought operation that enables the understanding of social reality beyond what is empirically observable or experienced. This study reveals that there were a number of necessary (internal) and contingent (external) factors that enabled or constrained networking, professional development and institutionalisation of environmental education courses in the context of the CDN. The key factors included existing cultural capital, donor political economy, power relations, poverty related factors and social transformation trends. The study found that relational approaches and the use of three relational theoretical lenses provided a broader lens which enabled this study to identify different dynamics, greater ontological depth and understanding of the relational dynamics and relations at play in the CDN beyond the participants' experience and observable events. The study also contends that networking can provide a support structure for social transformation and change in environmental education.
- Full Text:
- Date Issued: 2007
On the characterization of photovoltaic devices for concentrator purposes
- Authors: Vorster, Frederick Jacobus
- Date: 2007
- Subjects: Photovoltaic cells , Image processing , Solar cells
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10530 , http://hdl.handle.net/10948/639 , Photovoltaic cells , Image processing , Solar cells
- Description: This study originated from an evaluation of the performance of a commercially available high concentration point focus concentrator PV system. The effect of module design flaws was studied by using current-voltage (I-V) curves obtained from each module in the array. The position of reverse bias steps revealed the severity of mismatch in a string of series-connected cells. By understanding the effects of the various types of mismatch, power losses and damage to the solar cells resulting from hot spot formation can be minimized and several recommendations for improving the basic performance of similar systems were made. Concern over the extent and type of defect failure of the concentrator photovoltaic (CPV) cells prompted an investigation into the use of a light beam induced current (LBIC) technique to investigate the spatial distribution of defects. An overview of current and developing LBIC techniques revealed that the original standard LBIC techniques have found widespread application, and that far-reaching and important developments of the technique have taken place over the years. These developments are driven by natural progression as well as the availability of newly developed advanced measurement equipment. Several techniques such as Lock-in hermography and the use of infrared cameras have developed as complementary techniques to advanced LBIC techniques. As an accurate contactless evaluation tool that is able to image spatially distributed defects in cell material, the basis of this method seemed promising for the evaluation of concentrator cells.
- Full Text:
- Date Issued: 2007
- Authors: Vorster, Frederick Jacobus
- Date: 2007
- Subjects: Photovoltaic cells , Image processing , Solar cells
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10530 , http://hdl.handle.net/10948/639 , Photovoltaic cells , Image processing , Solar cells
- Description: This study originated from an evaluation of the performance of a commercially available high concentration point focus concentrator PV system. The effect of module design flaws was studied by using current-voltage (I-V) curves obtained from each module in the array. The position of reverse bias steps revealed the severity of mismatch in a string of series-connected cells. By understanding the effects of the various types of mismatch, power losses and damage to the solar cells resulting from hot spot formation can be minimized and several recommendations for improving the basic performance of similar systems were made. Concern over the extent and type of defect failure of the concentrator photovoltaic (CPV) cells prompted an investigation into the use of a light beam induced current (LBIC) technique to investigate the spatial distribution of defects. An overview of current and developing LBIC techniques revealed that the original standard LBIC techniques have found widespread application, and that far-reaching and important developments of the technique have taken place over the years. These developments are driven by natural progression as well as the availability of newly developed advanced measurement equipment. Several techniques such as Lock-in hermography and the use of infrared cameras have developed as complementary techniques to advanced LBIC techniques. As an accurate contactless evaluation tool that is able to image spatially distributed defects in cell material, the basis of this method seemed promising for the evaluation of concentrator cells.
- Full Text:
- Date Issued: 2007
On the microstructure and physical properties of hot pressed (Hf, Ti) C
- Authors: Heiligers, Christiané
- Date: 2007
- Subjects: Transition metal carbides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10532 , http://hdl.handle.net/10948/523 , http://hdl.handle.net/10948/d1011722 , Transition metal carbides
- Description: The microstructure and physical properties of hot pressed (Hf, Ti) C have been investigated with the aim of producing a cutting tool material with similar hardness to that of WC-Co and TiC-based cermets. Sintered samples were hot pressed from HfC0.7 and TiC0.9 powders using powder metallurgical techniques and the processing cycle was optimized for this system. Ni was used as a binder in selected samples and C black was added to compensate for sub-stoichiometry and to aid in the reduction of oxides formed during milling. Microstructural analyses were performed by scanning and transmission electron microscopy (SEM and TEM) and the composition was determined from X-ray diffraction (XRD) and energy dispersive X-ray spectrometry (EDS). The physical properties measured are density and Vickers hardness, and the indentation fracture toughness was determined using the Shetty formula. The fundamental interactions between HfC, TiC and Ni during hot pressing were investigated and the results obtained used to explain the microstructure that develops in samples made from powder mixtures. The interactions studied are the inter-diffusion of HfC and TiC through the solid state, and the dissolution and re-precipitation rate of the carbides in a liquid Ni binder. EDS analysis revealed that the rate at which Ti diffuses into HfC is higher than the rate at which Hf diffuses into TiC. Upper limits to the diffusion coefficients for these processes are determined and show that solid solution carbides will form from HfC + TiC powder mixtures at 2000 ºC in 1 hour if the average powder particle size is less than 5 μm. The diffusion rates decrease with a decrease in hot pressing temperature but mass transport between the phases can be enhanced by addition of a metallic binder. TEM and EDS analysis shows that Ni wets TiC more efficiently than HfC and that the solubility of TiC in Ni is also higher than that of HfC. The grain size of the carbide phases increases with an increase in the rate at which they dissolve into and re-precipitate from the liquid binder. The crystal structure of the binder phase depends on the concentration of Ti and Hf that remain in the binder after cooling and the carbide phase in which the binder is embedded. Analysis of TEM electron diffraction patterns show that the binder phase consists of cubic solid solutions as well as intermetallic and cubic phases in which atomic ordering is observed.
- Full Text:
- Date Issued: 2007
- Authors: Heiligers, Christiané
- Date: 2007
- Subjects: Transition metal carbides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10532 , http://hdl.handle.net/10948/523 , http://hdl.handle.net/10948/d1011722 , Transition metal carbides
- Description: The microstructure and physical properties of hot pressed (Hf, Ti) C have been investigated with the aim of producing a cutting tool material with similar hardness to that of WC-Co and TiC-based cermets. Sintered samples were hot pressed from HfC0.7 and TiC0.9 powders using powder metallurgical techniques and the processing cycle was optimized for this system. Ni was used as a binder in selected samples and C black was added to compensate for sub-stoichiometry and to aid in the reduction of oxides formed during milling. Microstructural analyses were performed by scanning and transmission electron microscopy (SEM and TEM) and the composition was determined from X-ray diffraction (XRD) and energy dispersive X-ray spectrometry (EDS). The physical properties measured are density and Vickers hardness, and the indentation fracture toughness was determined using the Shetty formula. The fundamental interactions between HfC, TiC and Ni during hot pressing were investigated and the results obtained used to explain the microstructure that develops in samples made from powder mixtures. The interactions studied are the inter-diffusion of HfC and TiC through the solid state, and the dissolution and re-precipitation rate of the carbides in a liquid Ni binder. EDS analysis revealed that the rate at which Ti diffuses into HfC is higher than the rate at which Hf diffuses into TiC. Upper limits to the diffusion coefficients for these processes are determined and show that solid solution carbides will form from HfC + TiC powder mixtures at 2000 ºC in 1 hour if the average powder particle size is less than 5 μm. The diffusion rates decrease with a decrease in hot pressing temperature but mass transport between the phases can be enhanced by addition of a metallic binder. TEM and EDS analysis shows that Ni wets TiC more efficiently than HfC and that the solubility of TiC in Ni is also higher than that of HfC. The grain size of the carbide phases increases with an increase in the rate at which they dissolve into and re-precipitate from the liquid binder. The crystal structure of the binder phase depends on the concentration of Ti and Hf that remain in the binder after cooling and the carbide phase in which the binder is embedded. Analysis of TEM electron diffraction patterns show that the binder phase consists of cubic solid solutions as well as intermetallic and cubic phases in which atomic ordering is observed.
- Full Text:
- Date Issued: 2007
Opportunity and constraint : historicity, hybridity and notions of cultural identity among farm workers in the Sundays River Valley
- Authors: Connor, Teresa Kathleen
- Date: 2007
- Subjects: Agricultural laborers -- South Africa -- Relocation Addo Elephant National Park (South Africa) Xhosa (African people) -- South Africa -- Eastern Cape -- Ethnic identity Forced migration -- South Africa -- Eastern Cape Land settlement -- South Africa -- Government policy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2117 , http://hdl.handle.net/10962/d1008367
- Description: This thesis focuses on relationships of opportunity and constraint among farm workers in the Sundays River Valley (SRV), Eastern Cape Province. Relationships of 'constraint' include those experiences of displacement and forced removal and war, including forced removals by the apartheid state in 1960 and 1970. Relationships of 'opportunity' include the ways in which residents in the SRV have contested their experiences of upheaval and domination, and the formation of a regional sense of place and belonging/ investigate how farm workers actually draw elements of locality and identity from their experiences of upheaval, and how displacement bolsters feelings of belonging and place. Instead of viewing displacement as a once-off experience, this thesis investigates displacement in historical terms, as a long-term, 'serial' experience of human movement, which is continued in the present- specifically through the creation of the Greater Addo Elephant National Park. I concentrate on developing a spatialised and cultural notion of movemenUplacement. 'Place' is investigated as a term that refers to rather indeterminate feelings of nostalgia, memory and identity, which depend on a particular connection to territory (ie: 'space'). I emphasise that elements of place in the SRV are drawn from and expressed along dualistic lines, which juxtapose situations of opportunity and constraint. In this way, farm workers' sense of connection to farms and ancestral territory in the SRV depends on their experiences of stable residency and work on farms, as well as their memories of removal from land in the area. I emphasise that those elements of conservatism (expressed as 'tradition' and Redness) among Xhosa-speaking farm workers are indications of a certain hybridity of identity in the region, which depend on differentiation from other groups (such as so-called 'coloured' farm workers and 'white' farmers), as well as associations between these groups. This thesis lays emphasis upon those less visible and definable 'identities' in the Eastern Cape Province, specifically by shifting focus away from the exhomeland states of the Ciskei and Transkei, to more marginal expressions of identity and change (among farm workers) in the Province. I point out that labourers cannot solely be defined by their positions as farm workers, but by their place and sense of cultural belonging in the area. In this sense, I use the idea of work as a loaded concept that can comment on a range of cultural attitudes towards belonging and place, and which is firmly embedded in the private lives of labourers - beyond their simple socio-economic conditions of farm work. I use Bourdieu's conception of habitus and doxa to define work as a set of dispositions that have been historicised and internalised by workers to such an extent, that relationships of domination are sometimes inadvertently obscured through their apparent 'naturalness'. Moreover, I point out that work can be related to ritualised action in the SRV through the use of performance and practice-based anthropological theory. Both work and ritual are symbolic actions, and are sites of struggle within which workers express themselves dualistically. Rituals, specifically, are dramatic events that combine disharmonious and harmonious social processes - juxtaposing the powerlessness of workers (on farms), and the deep sense of belonging and place in the SRV. I argue that the deep historical connections in the SRV have largely been ignored by conservationists in the drive to establish new protected zones (such as the Greater Addo Elephant National Park), and that a new model of shared conservation management is needed for this Park.
- Full Text:
- Date Issued: 2007
- Authors: Connor, Teresa Kathleen
- Date: 2007
- Subjects: Agricultural laborers -- South Africa -- Relocation Addo Elephant National Park (South Africa) Xhosa (African people) -- South Africa -- Eastern Cape -- Ethnic identity Forced migration -- South Africa -- Eastern Cape Land settlement -- South Africa -- Government policy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2117 , http://hdl.handle.net/10962/d1008367
- Description: This thesis focuses on relationships of opportunity and constraint among farm workers in the Sundays River Valley (SRV), Eastern Cape Province. Relationships of 'constraint' include those experiences of displacement and forced removal and war, including forced removals by the apartheid state in 1960 and 1970. Relationships of 'opportunity' include the ways in which residents in the SRV have contested their experiences of upheaval and domination, and the formation of a regional sense of place and belonging/ investigate how farm workers actually draw elements of locality and identity from their experiences of upheaval, and how displacement bolsters feelings of belonging and place. Instead of viewing displacement as a once-off experience, this thesis investigates displacement in historical terms, as a long-term, 'serial' experience of human movement, which is continued in the present- specifically through the creation of the Greater Addo Elephant National Park. I concentrate on developing a spatialised and cultural notion of movemenUplacement. 'Place' is investigated as a term that refers to rather indeterminate feelings of nostalgia, memory and identity, which depend on a particular connection to territory (ie: 'space'). I emphasise that elements of place in the SRV are drawn from and expressed along dualistic lines, which juxtapose situations of opportunity and constraint. In this way, farm workers' sense of connection to farms and ancestral territory in the SRV depends on their experiences of stable residency and work on farms, as well as their memories of removal from land in the area. I emphasise that those elements of conservatism (expressed as 'tradition' and Redness) among Xhosa-speaking farm workers are indications of a certain hybridity of identity in the region, which depend on differentiation from other groups (such as so-called 'coloured' farm workers and 'white' farmers), as well as associations between these groups. This thesis lays emphasis upon those less visible and definable 'identities' in the Eastern Cape Province, specifically by shifting focus away from the exhomeland states of the Ciskei and Transkei, to more marginal expressions of identity and change (among farm workers) in the Province. I point out that labourers cannot solely be defined by their positions as farm workers, but by their place and sense of cultural belonging in the area. In this sense, I use the idea of work as a loaded concept that can comment on a range of cultural attitudes towards belonging and place, and which is firmly embedded in the private lives of labourers - beyond their simple socio-economic conditions of farm work. I use Bourdieu's conception of habitus and doxa to define work as a set of dispositions that have been historicised and internalised by workers to such an extent, that relationships of domination are sometimes inadvertently obscured through their apparent 'naturalness'. Moreover, I point out that work can be related to ritualised action in the SRV through the use of performance and practice-based anthropological theory. Both work and ritual are symbolic actions, and are sites of struggle within which workers express themselves dualistically. Rituals, specifically, are dramatic events that combine disharmonious and harmonious social processes - juxtaposing the powerlessness of workers (on farms), and the deep sense of belonging and place in the SRV. I argue that the deep historical connections in the SRV have largely been ignored by conservationists in the drive to establish new protected zones (such as the Greater Addo Elephant National Park), and that a new model of shared conservation management is needed for this Park.
- Full Text:
- Date Issued: 2007
Passing the spear : a grounded theory study of the influence of family business value sets on succession planning in black family-owned businesses
- Authors: Musengi, Sandra
- Date: 2007
- Subjects: Grounded theory Family-owned business enterprises Family-owned business enterprises -- Succession Family-owned business enterprises -- Management Business enterprises, Black Business planning
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1192 , http://hdl.handle.net/10962/d1007056
- Description: Literature suggests that a small number of family businesses are able to make the transition from the founder to other family members with a common reason cited being the lack of planning. This study aims to build understanding of leadership succession in family businesses by focusing on the influence of a founder's family business value set on the succession planning process in Black family-owned businesses. Using the Strauss and Corbin (1990) grounded theory method, this study develops a theory of succession planning of Black familyowned businesses labelled Passing the Spear which is comprised of three stages based on an analysis of 21 qualitative interviews. The Spear was an analogy used to represent both the values of the founder and the family business, thus in Passing the Spear, founders where essentially performing a dual transfer of their values and leadership to the successor. The implementation of the process Passing the Spear was influenced by the family business value set of the founder, which in this study, were labelled as Traditional, Progressive, and Transitional. These value sets were distinguished by their behaviour regarding their choice of successor where founders with a traditional value set exhibited gatekeeping behaviour, while the behaviour of founders with a progressive value set was labelled navigating, and finally, founders with a transitional value set demonstrated behaviour labelled exploring. Furthermore, it was found that after the successor had been chosen, founders appeared to follow a generic succession planning process, however, the ease and timing of implementation was influenced by the family business context, evidence of being proactive and the degree of family business resilience. The process of Passing the Spear comprised of three stages of (a) Showing the spear where the founder's focus is on the induction and socialization of the successor using the strategies of bringing the successor into the family business and managing the family-business interface using relationships; (b) Explaining the spear entailed founders using the strategies of sharing knowledge and teaching the successors about the family business; and ( c) Sharing the spear was where founders focused on empowering the successors by implementing strategies of sharing responsibilities and learning from the successor.The process provides insights into the influence of family business values on the succession planning process and can be useful for founders of Black family-owned businesses in planning for succession in their businesses. In addition, the study provides another perspective of succession planning and offers a contribution to the literature for understanding succession in Black family-owned businesses.
- Full Text:
- Date Issued: 2007
- Authors: Musengi, Sandra
- Date: 2007
- Subjects: Grounded theory Family-owned business enterprises Family-owned business enterprises -- Succession Family-owned business enterprises -- Management Business enterprises, Black Business planning
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1192 , http://hdl.handle.net/10962/d1007056
- Description: Literature suggests that a small number of family businesses are able to make the transition from the founder to other family members with a common reason cited being the lack of planning. This study aims to build understanding of leadership succession in family businesses by focusing on the influence of a founder's family business value set on the succession planning process in Black family-owned businesses. Using the Strauss and Corbin (1990) grounded theory method, this study develops a theory of succession planning of Black familyowned businesses labelled Passing the Spear which is comprised of three stages based on an analysis of 21 qualitative interviews. The Spear was an analogy used to represent both the values of the founder and the family business, thus in Passing the Spear, founders where essentially performing a dual transfer of their values and leadership to the successor. The implementation of the process Passing the Spear was influenced by the family business value set of the founder, which in this study, were labelled as Traditional, Progressive, and Transitional. These value sets were distinguished by their behaviour regarding their choice of successor where founders with a traditional value set exhibited gatekeeping behaviour, while the behaviour of founders with a progressive value set was labelled navigating, and finally, founders with a transitional value set demonstrated behaviour labelled exploring. Furthermore, it was found that after the successor had been chosen, founders appeared to follow a generic succession planning process, however, the ease and timing of implementation was influenced by the family business context, evidence of being proactive and the degree of family business resilience. The process of Passing the Spear comprised of three stages of (a) Showing the spear where the founder's focus is on the induction and socialization of the successor using the strategies of bringing the successor into the family business and managing the family-business interface using relationships; (b) Explaining the spear entailed founders using the strategies of sharing knowledge and teaching the successors about the family business; and ( c) Sharing the spear was where founders focused on empowering the successors by implementing strategies of sharing responsibilities and learning from the successor.The process provides insights into the influence of family business values on the succession planning process and can be useful for founders of Black family-owned businesses in planning for succession in their businesses. In addition, the study provides another perspective of succession planning and offers a contribution to the literature for understanding succession in Black family-owned businesses.
- Full Text:
- Date Issued: 2007
Pharmaceutical analysis and quality of complementary medicines : sceletium and associated products
- Patnala, Satya Siva Rama Ranganath Srinivas
- Authors: Patnala, Satya Siva Rama Ranganath Srinivas
- Date: 2007
- Subjects: Alternative medicine , Herbs -- Therapeutic use
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3872 , http://hdl.handle.net/10962/d1018263
- Description: There has been an upsurge in the use of Complementary and Alternate Medicines (CAMs) in both developed and developing countries. Although herbal medicines have been in use for many centuries, their quality, safety and efficacy are still of major concern. Many countries are in the process of integrating CAMs into conventional health care systems based on the knowledge and use of traditional medicines. The quality control (QC) of herbal products usually presents a formidable analytical challenge in view of the complexity of the constituents in plant material and the commercial non-availability of appropriate qualified reference standards. Sceletium, a genus belonging to the family Aizoaceae, has been reported to contain psychoactive alkaloids, specifically mesembrine, mesembrenone, mesembrenol and some other related alkaloids. Sceletium is marketed as dried plant powder and as phyto-pharmaceutical dosage forms. Sceletium products and plant material marketed through health shops and on the internet are associated with unjustified claims of specific therapeutic efficacy and may be of dubious quality. Validated analytical methods to estimate Sceletium alkaloids have not previously been reported in the scientific literature and the available methods have focused only on qualitative estimation. Furthermore, since appropriate markers were not commercially available for use as reference standards, a primary objective of this study was to isolate relevant compounds, qualify them as reference standards which could be applied to develop appropriate validated qualitative and quantitative analytical methods for fingerprinting and assay of Sceletium plant material and dosage forms. The alkaloidal markers mesembrine, mesembrenone and ∆⁷ mesembrenone were isolated by solvent extraction and chromatography from dried plant material. Mesembranol and epimesembranol were synthesised by hydrogenation of the isolated mesembrine using the catalyst platinum (IV) oxide and then further purified by semi-preparative column chromatography. All compounds were subjected to analysis by ¹H, ¹³C, 2-D nuclear magnetic resonance and liquid chromatography-tandem mass spectroscopy. Mesembrine was converted to hydrochloride crystals and mesembranol was isolated as crystals from the hydrogenation reaction mass. These compounds were analysed and characterised by X-ray crystallography. A relatively simple HPLC method for the separation and quantitative analysis of five relevant alkaloidal components in Sceletium was developed and validated. The method was applied to determine the alkaloids in plant material and dosage forms containing Sceletium. An LCMS method developed during the study provided accurate identification of the five relevant Sceletium alkaloids. The method was applied for the quantitative analysis and QC of Sceletium plant material and its dosage forms. This LCMS method was found to efficiently ionize the relevant alkaloidal markers in order to facilitate their detection, identification and quantification in Sceletium plant material as well as for the assay and QC of dosage forms containing Sceletium. The chemotaxonomy of some Sceletium species and commercially available Sceletium dosage forms were successfully studied by the LCMS method. The HPLC and LCMS methods were also used to monitor the bio-conversion of some of the alkaloids while processing the plant material as per traditional method of fermentation. Additionally a high resolution CZE method was developed for the separation of several Sceletium alkaloids in relatively short analysis times. This analytical method was used successfully to fingerprint the alkaloids and quantify mesembrine in Sceletium and its products. Sceletium species grown under varying conditions at different locations, when analyzed, showed major differences in their composition of alkaloids and an enormous difference was found to exist between the various species with respect to the presence and content of alkaloids. Sceletium and its products marketed through health shops and the internet may thus have problems with respect to the quality and related therapeutic efficacy. The QC of Sceletium presents a formidable challenge as Sceletium plants and products contain a complex mixture of compounds. The work presented herein contributes to a growing body of scientific knowledge to improve the QC standards of herbal medicines and also to provide vital information regarding the selection of plant species and information on the specific alkaloidal constituents to the cultivators of Sceletium and the manufacturers of its products.
- Full Text:
- Date Issued: 2007
- Authors: Patnala, Satya Siva Rama Ranganath Srinivas
- Date: 2007
- Subjects: Alternative medicine , Herbs -- Therapeutic use
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3872 , http://hdl.handle.net/10962/d1018263
- Description: There has been an upsurge in the use of Complementary and Alternate Medicines (CAMs) in both developed and developing countries. Although herbal medicines have been in use for many centuries, their quality, safety and efficacy are still of major concern. Many countries are in the process of integrating CAMs into conventional health care systems based on the knowledge and use of traditional medicines. The quality control (QC) of herbal products usually presents a formidable analytical challenge in view of the complexity of the constituents in plant material and the commercial non-availability of appropriate qualified reference standards. Sceletium, a genus belonging to the family Aizoaceae, has been reported to contain psychoactive alkaloids, specifically mesembrine, mesembrenone, mesembrenol and some other related alkaloids. Sceletium is marketed as dried plant powder and as phyto-pharmaceutical dosage forms. Sceletium products and plant material marketed through health shops and on the internet are associated with unjustified claims of specific therapeutic efficacy and may be of dubious quality. Validated analytical methods to estimate Sceletium alkaloids have not previously been reported in the scientific literature and the available methods have focused only on qualitative estimation. Furthermore, since appropriate markers were not commercially available for use as reference standards, a primary objective of this study was to isolate relevant compounds, qualify them as reference standards which could be applied to develop appropriate validated qualitative and quantitative analytical methods for fingerprinting and assay of Sceletium plant material and dosage forms. The alkaloidal markers mesembrine, mesembrenone and ∆⁷ mesembrenone were isolated by solvent extraction and chromatography from dried plant material. Mesembranol and epimesembranol were synthesised by hydrogenation of the isolated mesembrine using the catalyst platinum (IV) oxide and then further purified by semi-preparative column chromatography. All compounds were subjected to analysis by ¹H, ¹³C, 2-D nuclear magnetic resonance and liquid chromatography-tandem mass spectroscopy. Mesembrine was converted to hydrochloride crystals and mesembranol was isolated as crystals from the hydrogenation reaction mass. These compounds were analysed and characterised by X-ray crystallography. A relatively simple HPLC method for the separation and quantitative analysis of five relevant alkaloidal components in Sceletium was developed and validated. The method was applied to determine the alkaloids in plant material and dosage forms containing Sceletium. An LCMS method developed during the study provided accurate identification of the five relevant Sceletium alkaloids. The method was applied for the quantitative analysis and QC of Sceletium plant material and its dosage forms. This LCMS method was found to efficiently ionize the relevant alkaloidal markers in order to facilitate their detection, identification and quantification in Sceletium plant material as well as for the assay and QC of dosage forms containing Sceletium. The chemotaxonomy of some Sceletium species and commercially available Sceletium dosage forms were successfully studied by the LCMS method. The HPLC and LCMS methods were also used to monitor the bio-conversion of some of the alkaloids while processing the plant material as per traditional method of fermentation. Additionally a high resolution CZE method was developed for the separation of several Sceletium alkaloids in relatively short analysis times. This analytical method was used successfully to fingerprint the alkaloids and quantify mesembrine in Sceletium and its products. Sceletium species grown under varying conditions at different locations, when analyzed, showed major differences in their composition of alkaloids and an enormous difference was found to exist between the various species with respect to the presence and content of alkaloids. Sceletium and its products marketed through health shops and the internet may thus have problems with respect to the quality and related therapeutic efficacy. The QC of Sceletium presents a formidable challenge as Sceletium plants and products contain a complex mixture of compounds. The work presented herein contributes to a growing body of scientific knowledge to improve the QC standards of herbal medicines and also to provide vital information regarding the selection of plant species and information on the specific alkaloidal constituents to the cultivators of Sceletium and the manufacturers of its products.
- Full Text:
- Date Issued: 2007
Phthalocyanines : photochemical, electrochemical and biomimetic catalytic behaviour
- Authors: Sehlotho, Nthapo
- Date: 2007
- Subjects: Phthalocyanines Photochemistry Electrochemistry Biomimetics Oxidation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4336 , http://hdl.handle.net/10962/d1004997
- Description: This thesis explored use of metallophthalocyanines as electrocatalysts towards thiol and thiocyanate oxidation, nitrosothiol decomposition and reduction of oxygen, as well as biomimetic and photo-catalysts of cyclohexene oxidation. 2-mercaptoethanol (2-ME), L-cysteine (CYS) and reduced glutathione (GSH) thiols were oxidized on cobalt tetra ethoxythiophene and cobalt tetra phenoxy pyrrole phthalocyanine modified glassy carbon electrodes, whose catalytic activity was found to depend on pH, film thickness and method of electrode modification. Oxidation of thiocyanate (SCN-), CYS and 2-ME was catalyzed by a selfassembled monolayer of cobalt tetraethoxythiophene Thiocyanate oxidation occurred via two electron transfer, whereas that of CYS and 2-ME required 1 electron. The oxidations of SCN- and 2-ME were catalyzed by ring based processes, while CYS was catalyzed by both Co[superscript III]/Co[superscript II] process and ring-based processes. Oxidation of GSH and 2-ME was conducted on screen printed graphite electrodes modified with cobalt phthalocyanine. Activity depended on method of electrode modification and CoPc % composition. Decomposition of Snitrosoglutathione occurred in the presence of copper ions and NaBH[subscript 4]. Reduced and oxidized glutathione were detected as products using cobalt phthalocyanine adsorbed on an ordinary pyrolytic graphite electrode. Reduction of oxygen was electro-catalyzed by adsorbed manganese phthalocyanine complexes on glassy carbon electrodes. FePc, FePc(Cl)[subscript 16], CoPc and CoPc substituted with phenoxypyrrole and ethoxythiophene ligands were also used as electro-catalysts. Oxygen reduction occurred via two electron transfer in acidic and neutral media forming hydrogen peroxide, while water was formed in basic media via four electron transfer. Cyclohexene oxidation using tert-butylhydroperoxide or chloroperoxy benzoic acid as oxidants in the presence of FePc, FePc(Cl)[subscript 16] and CoPc formed cyclohexene oxide, 2-cyclohexen-1-ol, 2- cyclohexen-1-one and adipic acid. Product selectivity depended on the nature of catalyst and oxidant. The FePc(Cl)[subscript 16] catalyst was transformed into a µ-oxo dimer during the oxidation process while M[superscript III]Pc intermediates were formed with Co[superscript II]Pc and Fe[superscript II]Pc catalysts. Cyclohexene photooxidation catalyzed by zinc phthalocyanine using either red or white light formed 2-cyclohexen-1-one, 2-cyclohexen-1-ol, transcyclohexane diol, cyclohexene oxide and cyclohexene hydroperoxide via singlet oxygen and radical mechanisms. Product yields depended on the light wavelength and intensity, solvent, irradiation time and the rate of photodegradation of the catalyst.
- Full Text:
- Date Issued: 2007
- Authors: Sehlotho, Nthapo
- Date: 2007
- Subjects: Phthalocyanines Photochemistry Electrochemistry Biomimetics Oxidation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4336 , http://hdl.handle.net/10962/d1004997
- Description: This thesis explored use of metallophthalocyanines as electrocatalysts towards thiol and thiocyanate oxidation, nitrosothiol decomposition and reduction of oxygen, as well as biomimetic and photo-catalysts of cyclohexene oxidation. 2-mercaptoethanol (2-ME), L-cysteine (CYS) and reduced glutathione (GSH) thiols were oxidized on cobalt tetra ethoxythiophene and cobalt tetra phenoxy pyrrole phthalocyanine modified glassy carbon electrodes, whose catalytic activity was found to depend on pH, film thickness and method of electrode modification. Oxidation of thiocyanate (SCN-), CYS and 2-ME was catalyzed by a selfassembled monolayer of cobalt tetraethoxythiophene Thiocyanate oxidation occurred via two electron transfer, whereas that of CYS and 2-ME required 1 electron. The oxidations of SCN- and 2-ME were catalyzed by ring based processes, while CYS was catalyzed by both Co[superscript III]/Co[superscript II] process and ring-based processes. Oxidation of GSH and 2-ME was conducted on screen printed graphite electrodes modified with cobalt phthalocyanine. Activity depended on method of electrode modification and CoPc % composition. Decomposition of Snitrosoglutathione occurred in the presence of copper ions and NaBH[subscript 4]. Reduced and oxidized glutathione were detected as products using cobalt phthalocyanine adsorbed on an ordinary pyrolytic graphite electrode. Reduction of oxygen was electro-catalyzed by adsorbed manganese phthalocyanine complexes on glassy carbon electrodes. FePc, FePc(Cl)[subscript 16], CoPc and CoPc substituted with phenoxypyrrole and ethoxythiophene ligands were also used as electro-catalysts. Oxygen reduction occurred via two electron transfer in acidic and neutral media forming hydrogen peroxide, while water was formed in basic media via four electron transfer. Cyclohexene oxidation using tert-butylhydroperoxide or chloroperoxy benzoic acid as oxidants in the presence of FePc, FePc(Cl)[subscript 16] and CoPc formed cyclohexene oxide, 2-cyclohexen-1-ol, 2- cyclohexen-1-one and adipic acid. Product selectivity depended on the nature of catalyst and oxidant. The FePc(Cl)[subscript 16] catalyst was transformed into a µ-oxo dimer during the oxidation process while M[superscript III]Pc intermediates were formed with Co[superscript II]Pc and Fe[superscript II]Pc catalysts. Cyclohexene photooxidation catalyzed by zinc phthalocyanine using either red or white light formed 2-cyclohexen-1-one, 2-cyclohexen-1-ol, transcyclohexane diol, cyclohexene oxide and cyclohexene hydroperoxide via singlet oxygen and radical mechanisms. Product yields depended on the light wavelength and intensity, solvent, irradiation time and the rate of photodegradation of the catalyst.
- Full Text:
- Date Issued: 2007
Prime near-ring modules and their links with the generalised group near-ring
- Authors: Juglal, Shaanraj
- Date: 2007
- Subjects: Near-rings
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10507 , http://hdl.handle.net/10948/714 , Near-rings
- Description: In view of the facts that the definition of a ring led to the definition of a near- ring, the definition of a ring module led to the definition of a near-ring module, prime rings resulted in investigations with respect to primeness in near-rings, one is naturally inclined to attempt to define the notion of a group near-ring seeing that the group ring had already been defined and investigated into by, interalia, Groenewald in [7] . However, in trying to define the group near-ring along the same lines as the group ring was defined, it was found that the resulting multiplication was, in general, not associative in the near-ring case due to the lack of one distributive property. In 1976, Meldrum [19] achieved success in defining the group near-ring. How- ever, in his definition, only distributively generated near-rings were considered and the distributive generators played a vital role in the construction. In 1989, Le Riche, Meldrum and van der Walt [17], adopted a similar approach to that which led to a successful and fruitful definition of matrix near-rings, and defined the group near-ring in a more general sense. In particular, they defined R[G], the group near-ring of a group G over a near-ring R, as a subnear-ring of M(RG), the near-ring of all mappings of the group RG into itself. More recently, Groenewald and Lee [14], further generalised the definition of R[G] to R[S : M], the generalised semigroup near-ring of a semigroup S over any faithful R-module M. Again, the natural thing to do would be to extend the results obtained for R[G] to R[S : M], and this they achieved with much success.
- Full Text:
- Date Issued: 2007
- Authors: Juglal, Shaanraj
- Date: 2007
- Subjects: Near-rings
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10507 , http://hdl.handle.net/10948/714 , Near-rings
- Description: In view of the facts that the definition of a ring led to the definition of a near- ring, the definition of a ring module led to the definition of a near-ring module, prime rings resulted in investigations with respect to primeness in near-rings, one is naturally inclined to attempt to define the notion of a group near-ring seeing that the group ring had already been defined and investigated into by, interalia, Groenewald in [7] . However, in trying to define the group near-ring along the same lines as the group ring was defined, it was found that the resulting multiplication was, in general, not associative in the near-ring case due to the lack of one distributive property. In 1976, Meldrum [19] achieved success in defining the group near-ring. How- ever, in his definition, only distributively generated near-rings were considered and the distributive generators played a vital role in the construction. In 1989, Le Riche, Meldrum and van der Walt [17], adopted a similar approach to that which led to a successful and fruitful definition of matrix near-rings, and defined the group near-ring in a more general sense. In particular, they defined R[G], the group near-ring of a group G over a near-ring R, as a subnear-ring of M(RG), the near-ring of all mappings of the group RG into itself. More recently, Groenewald and Lee [14], further generalised the definition of R[G] to R[S : M], the generalised semigroup near-ring of a semigroup S over any faithful R-module M. Again, the natural thing to do would be to extend the results obtained for R[G] to R[S : M], and this they achieved with much success.
- Full Text:
- Date Issued: 2007
Race against democracy: a case study of the Mail & Guardian during the early years of the Mbeki presidency, 1999-2002
- Authors: Steenveld, Lynette Noreen
- Date: 2007
- Subjects: Mbeki, Thabo -- In mass media , Mail & Guardian , South African newspapers , Press -- South Africa , Press and politics -- South Africa , Journalism -- South Africa , Mass media and race relations -- South Africa , Racism in the press -- South Africa , Mass media -- Political aspects -- South Africa , South Africa -- Politics and government -- 20th century
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3537 , http://hdl.handle.net/10962/d1015572
- Description: This thesis examines the 1998 complaint of racism against the Mail & Guardian, a leading exponent of South Africa's alternative press in the 1980s, and important contemporary producer of investigative journalism. The study is framed within a cultural studies approach, analysing the Mail & Guardian as constituted by a 'circuit of production': its social context, production, texts, and audiences. The thesis makes three main arguments. First, that the claim of racism cannot be understood outside of a consideration of both the changing political milieu, and subtle changes within the Mail & Guardian itself. Significant social changes relate to the reconfiguration of racial and class identities wrought by the 'Mbeki state'. Within the Mail & Guardian, the thesis argues for the importance of the power and subjectivity of the editor as a key 'factor' shaping the identity of the paper, evidenced in its production practices and textual outputs. In this regard, the thesis departs from a functionalist analysis of particular 'roles' within the newsroom, drawing instead on a post-structuralist approach to organisational studies. Based on this production and social context, the thesis examines key texts which deal with aspects of South Africa's social transformation, and which exemplify aspects of the Mail & Guardian's reporting which led to the complaint of racism by the Black Lawyers Association (BLA) and the Association of Black Accountants (ABASA). Their complaint was that the Mail & Guardian's reporting impugned the dignity of black people, and in so doing was a violation of their rights to dignity and equality which are constitutionally guaranteed. However, as freedom of the press is also guaranteed by the South African constitution, their complaint to the South African Human Rights Commission (SAHRC) resulted in public debate about these contending rights. My second argument relates to the jurisprudential approach to racism, and the related issue of affirmative action, which informed the complaint against the paper. Contrary to the 'normative', liberal approach to these issues, this thesis highlights Critical Race Theory as the jurisprudential basis for both the claimants' accusation of racism against the Mail & Guardian, and aspects of its implicit use in South African human rights adjudication. The thesis argues that in failing to recognise these different philosophical and political bases of legal reasoning, the media, including the Mail & Guardian, in reporting on these matters failed in their purported role of serving the public interest. The thesis concludes by applying Fraser's critique of Habermas's notion of a single, bourgeois public sphere to journalism, thereby suggesting ways in which the critiques of some of the Mail & Guardian's own journalists could be employed to enlarge its approach to journalism - giving voice to constituencies seldom heard in mainstream media.
- Full Text:
- Date Issued: 2007
- Authors: Steenveld, Lynette Noreen
- Date: 2007
- Subjects: Mbeki, Thabo -- In mass media , Mail & Guardian , South African newspapers , Press -- South Africa , Press and politics -- South Africa , Journalism -- South Africa , Mass media and race relations -- South Africa , Racism in the press -- South Africa , Mass media -- Political aspects -- South Africa , South Africa -- Politics and government -- 20th century
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3537 , http://hdl.handle.net/10962/d1015572
- Description: This thesis examines the 1998 complaint of racism against the Mail & Guardian, a leading exponent of South Africa's alternative press in the 1980s, and important contemporary producer of investigative journalism. The study is framed within a cultural studies approach, analysing the Mail & Guardian as constituted by a 'circuit of production': its social context, production, texts, and audiences. The thesis makes three main arguments. First, that the claim of racism cannot be understood outside of a consideration of both the changing political milieu, and subtle changes within the Mail & Guardian itself. Significant social changes relate to the reconfiguration of racial and class identities wrought by the 'Mbeki state'. Within the Mail & Guardian, the thesis argues for the importance of the power and subjectivity of the editor as a key 'factor' shaping the identity of the paper, evidenced in its production practices and textual outputs. In this regard, the thesis departs from a functionalist analysis of particular 'roles' within the newsroom, drawing instead on a post-structuralist approach to organisational studies. Based on this production and social context, the thesis examines key texts which deal with aspects of South Africa's social transformation, and which exemplify aspects of the Mail & Guardian's reporting which led to the complaint of racism by the Black Lawyers Association (BLA) and the Association of Black Accountants (ABASA). Their complaint was that the Mail & Guardian's reporting impugned the dignity of black people, and in so doing was a violation of their rights to dignity and equality which are constitutionally guaranteed. However, as freedom of the press is also guaranteed by the South African constitution, their complaint to the South African Human Rights Commission (SAHRC) resulted in public debate about these contending rights. My second argument relates to the jurisprudential approach to racism, and the related issue of affirmative action, which informed the complaint against the paper. Contrary to the 'normative', liberal approach to these issues, this thesis highlights Critical Race Theory as the jurisprudential basis for both the claimants' accusation of racism against the Mail & Guardian, and aspects of its implicit use in South African human rights adjudication. The thesis argues that in failing to recognise these different philosophical and political bases of legal reasoning, the media, including the Mail & Guardian, in reporting on these matters failed in their purported role of serving the public interest. The thesis concludes by applying Fraser's critique of Habermas's notion of a single, bourgeois public sphere to journalism, thereby suggesting ways in which the critiques of some of the Mail & Guardian's own journalists could be employed to enlarge its approach to journalism - giving voice to constituencies seldom heard in mainstream media.
- Full Text:
- Date Issued: 2007
Regulation of hyu gene expression in Agrobacterium tumefaciens strains RU-AE01 and RU-OR
- Authors: Jiwaji, Meesbah
- Date: 2007
- Subjects: Agrobacterium tumefaciens Amino acids Gene expression Hydrolysis Hydantoin Enzymes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3983 , http://hdl.handle.net/10962/d1004042
- Description: Several Agrobacterium tumefaciens strains have been isolated for their ability to produce D-amino acids from D, L-substituted hydantoins. The optically pure D-amino acids are used in the synthesis of pharmaceuticals, as food additives and as insecticides. This hydrolysis of D, L-substituted hydantoins is catalysed by two hydantoin-hydrolyzing enzymes, an hydantoinase and an N-carbamyl amino acid amidohydrolase. While the hydantoin-hydrolyzing enzymes have been studied in detail, the mechanisms that control expression of the hyu genes have not. The research reported in this work elucidates some of the mechanisms involved in the regulation of the hyu genes in A. tumefaciens strains. The hydantoin-hydrolyzing enzyme activity from the environmental isolate A. tumefaciens RU-AE01 was characterized. A broad host range vector for the simultaneous analysis of divergent promoters was constructed. The promoter regions responsible for the activation of transcription of hyuH and hyuC were identified by deletion analysis. It was proposed that transcription of hyuH was activated by a putative σ[superscript 54]-dependent promoter or a putative σ[superscript 70]-dependent promoter identified upstream of the hyuH gene. The hyuC gene was activated by a putative σ[superscript 70]-dependent promoter identified upstream of the hyuC gene. The regulation of hydantoinase and N-carbamyl amino acid amidohydrolase enzyme activity was compared to the regulation of transcription from the RU-AE01 hyuH-hyuC region. Expression of the hydantoin-hydrolyzing enzymes was regulated by induction which correlated with reporter enzyme expression from the hyuH and hyuC promoter regions. However, the expression of the hydantoin-hydrolyzing enzymes was also regulated by nitrogen catabolite repression (NCR). This did not correlate to the reporter gene expression of the hyuH promoter region but did compare to the reporter gene expression of the hyuC promoter region. This suggested that NCR of hyuH was at the post-translational level whereas NCR of the hyuC promoter was at the transcriptional level. Pathways involved in the regulation of the hyu genes were characterized. The production of the hydantoin-hydrolyzing enzymes in both A. tumefaciens strains RU-AE01 and RU-OR were regulated by proteins involved in the global ntr pathway. The levels of the hydantoin-hydrolyzing enzymes in strain RU-AE01 were elevated in the presence of increased levels of NtrB and NtrC illustrating the importance of the ntr pathway in the regulation of the levels of the hydantoin-hydrolyzing enzymes. Similarly, in RU-OR the presence of exogenous NtrB and NtrC elevated levels of N-carbamyl amino acid amidohydrolase activity. However, the levels of hydantoinase enzyme activity in strain RU-OR were elevated in the presence of NtrC alone. In addition, the presence of a His6-tagged NtrC molecule abolished the elevation in the levels of the hydantoinase but not the N-carbamyl amino acid amidohydrolase enzyme activity in strain RU-OR. This suggests that NtrC has a direct role in the regulation of the expression of hyuH in RU-OR. In addition, it indicates that the hyu genes in the two A. tumefaciens strains RU-AE01 and RU-OR are different. The presence of the RU-AE01 hyuH-hyuC fragment caused a dramatic increase in the hydantoin-hydrolyzing enzyme activity in strain RU-OR but not strain RU-AE01. This implied the incidence of a possible repressor protein in RU-OR, which is titrated out by the presence of the RU-AE01 hyuH-hyuC fragment. Protein-DNA binding assays suggest that this putative repressor may be 38 kDa in RU-OR cells.
- Full Text:
- Date Issued: 2007
- Authors: Jiwaji, Meesbah
- Date: 2007
- Subjects: Agrobacterium tumefaciens Amino acids Gene expression Hydrolysis Hydantoin Enzymes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3983 , http://hdl.handle.net/10962/d1004042
- Description: Several Agrobacterium tumefaciens strains have been isolated for their ability to produce D-amino acids from D, L-substituted hydantoins. The optically pure D-amino acids are used in the synthesis of pharmaceuticals, as food additives and as insecticides. This hydrolysis of D, L-substituted hydantoins is catalysed by two hydantoin-hydrolyzing enzymes, an hydantoinase and an N-carbamyl amino acid amidohydrolase. While the hydantoin-hydrolyzing enzymes have been studied in detail, the mechanisms that control expression of the hyu genes have not. The research reported in this work elucidates some of the mechanisms involved in the regulation of the hyu genes in A. tumefaciens strains. The hydantoin-hydrolyzing enzyme activity from the environmental isolate A. tumefaciens RU-AE01 was characterized. A broad host range vector for the simultaneous analysis of divergent promoters was constructed. The promoter regions responsible for the activation of transcription of hyuH and hyuC were identified by deletion analysis. It was proposed that transcription of hyuH was activated by a putative σ[superscript 54]-dependent promoter or a putative σ[superscript 70]-dependent promoter identified upstream of the hyuH gene. The hyuC gene was activated by a putative σ[superscript 70]-dependent promoter identified upstream of the hyuC gene. The regulation of hydantoinase and N-carbamyl amino acid amidohydrolase enzyme activity was compared to the regulation of transcription from the RU-AE01 hyuH-hyuC region. Expression of the hydantoin-hydrolyzing enzymes was regulated by induction which correlated with reporter enzyme expression from the hyuH and hyuC promoter regions. However, the expression of the hydantoin-hydrolyzing enzymes was also regulated by nitrogen catabolite repression (NCR). This did not correlate to the reporter gene expression of the hyuH promoter region but did compare to the reporter gene expression of the hyuC promoter region. This suggested that NCR of hyuH was at the post-translational level whereas NCR of the hyuC promoter was at the transcriptional level. Pathways involved in the regulation of the hyu genes were characterized. The production of the hydantoin-hydrolyzing enzymes in both A. tumefaciens strains RU-AE01 and RU-OR were regulated by proteins involved in the global ntr pathway. The levels of the hydantoin-hydrolyzing enzymes in strain RU-AE01 were elevated in the presence of increased levels of NtrB and NtrC illustrating the importance of the ntr pathway in the regulation of the levels of the hydantoin-hydrolyzing enzymes. Similarly, in RU-OR the presence of exogenous NtrB and NtrC elevated levels of N-carbamyl amino acid amidohydrolase activity. However, the levels of hydantoinase enzyme activity in strain RU-OR were elevated in the presence of NtrC alone. In addition, the presence of a His6-tagged NtrC molecule abolished the elevation in the levels of the hydantoinase but not the N-carbamyl amino acid amidohydrolase enzyme activity in strain RU-OR. This suggests that NtrC has a direct role in the regulation of the expression of hyuH in RU-OR. In addition, it indicates that the hyu genes in the two A. tumefaciens strains RU-AE01 and RU-OR are different. The presence of the RU-AE01 hyuH-hyuC fragment caused a dramatic increase in the hydantoin-hydrolyzing enzyme activity in strain RU-OR but not strain RU-AE01. This implied the incidence of a possible repressor protein in RU-OR, which is titrated out by the presence of the RU-AE01 hyuH-hyuC fragment. Protein-DNA binding assays suggest that this putative repressor may be 38 kDa in RU-OR cells.
- Full Text:
- Date Issued: 2007
Severe convective storm risk in the Eastern Cape Province of South Africa
- Authors: Pyle, Desmond Mark
- Date: 2007
- Subjects: Storms -- South Africa -- Eastern Cape Natural disasters -- South Africa -- Eastern Cape Disasters -- South Africa -- Eastern Cape Disaster relief -- South Africa -- Eastern Cape -- Management Meteorology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4853 , http://hdl.handle.net/10962/d1005529
- Description: This study investigates the temporal, spatial and impact characteristics of severe convective storm hazard and risk in the Eastern Cape Province of South Africa. Using historical data on severe convective storms dating from 1897, patterns of the hazard threat and risk to various geographic populations were investigated. A conceptual framework that emphasises the combined role hazard and vulnerability play in defining risk was used for the study. A methodology for ranking the severity of the storms in the historical dataset, based on recorded damage/impact, was specifically developed for the study. It is intended that this methodology will have a potentially wider application and may be adapted to a range of hazard impact and risk studies in South Africa and internationally. The study was undertaken within the context of the South African Disaster Management Act of 2002. Findings of the study show that severe convective storms can occur throughout the province, but there are clearly demarcated areas of higher frequency and concentration. The impact of storms is particularly severe on impoverished and vulnerable rural populations in the eastern parts of the province, where there is an urgent need for building capacity in disaster risk management. A major outcome of the study is the production of a severe convective storm hazard/risk map of the Eastern Cape, which it is hoped will be of benefit to a number of stakeholders in the province, particularly disaster management, but also the South African Weather Service, agricultural organisations, development/planning authorities, educational authorities and risk insurers. It is hoped that this map and the study in general will assist in guiding the operational responses of the various authorities, especially in terms of those interventions aimed at disaster risk reduction in the Eastern Cape.
- Full Text:
- Date Issued: 2007
- Authors: Pyle, Desmond Mark
- Date: 2007
- Subjects: Storms -- South Africa -- Eastern Cape Natural disasters -- South Africa -- Eastern Cape Disasters -- South Africa -- Eastern Cape Disaster relief -- South Africa -- Eastern Cape -- Management Meteorology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4853 , http://hdl.handle.net/10962/d1005529
- Description: This study investigates the temporal, spatial and impact characteristics of severe convective storm hazard and risk in the Eastern Cape Province of South Africa. Using historical data on severe convective storms dating from 1897, patterns of the hazard threat and risk to various geographic populations were investigated. A conceptual framework that emphasises the combined role hazard and vulnerability play in defining risk was used for the study. A methodology for ranking the severity of the storms in the historical dataset, based on recorded damage/impact, was specifically developed for the study. It is intended that this methodology will have a potentially wider application and may be adapted to a range of hazard impact and risk studies in South Africa and internationally. The study was undertaken within the context of the South African Disaster Management Act of 2002. Findings of the study show that severe convective storms can occur throughout the province, but there are clearly demarcated areas of higher frequency and concentration. The impact of storms is particularly severe on impoverished and vulnerable rural populations in the eastern parts of the province, where there is an urgent need for building capacity in disaster risk management. A major outcome of the study is the production of a severe convective storm hazard/risk map of the Eastern Cape, which it is hoped will be of benefit to a number of stakeholders in the province, particularly disaster management, but also the South African Weather Service, agricultural organisations, development/planning authorities, educational authorities and risk insurers. It is hoped that this map and the study in general will assist in guiding the operational responses of the various authorities, especially in terms of those interventions aimed at disaster risk reduction in the Eastern Cape.
- Full Text:
- Date Issued: 2007
Spermiogenesis, sperm ultrastructure and reproductive tract morphology in cicadas implications for systematic relationships
- Chawanji, Abraham Simbarashe
- Authors: Chawanji, Abraham Simbarashe
- Date: 2007
- Subjects: Cicadas Cicadas -- Reproduction Spermiogenesis in animals
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5770 , http://hdl.handle.net/10962/d1005458
- Description: Sperm structure in five species of cicadine cicadas (Albanycada albigera, Azanicada zuluensis, Platypleura capensis, P. hirtipennis and Pycna semiclara) and five species of cicadettine cicadas (Melampsalta leucoptera, Quintilia walkeri, Stagira simplex, Xosopsaltria thunbergi and Monomatapa matoposa) was investigated by light and electron microscopy. In addition, spermiogenesis in cicadas was described; the information was derived from two cicadettines (Diceroprocta biconica and M. matoposa) and three cicadines (Kongota punctigera, P. capensis and P. semiclara). Mature spermatozoa of all species investigated are elongate and filiform, consisting of three distinct regions: the head (acrosome and nucleus), mid-piece and tail. All species produce more than one discrete length of nucleated, motile sperm, a form of sperm polymorphism termed polymegaly. Polymegaly is expressed in three ways; sperm have uni-, bi- or trimodal nucleus and tail lengths. Besides the differences in length, there are also notable differences in the size of nuclei. The anterior parts of sperm heads are embedded in an elongate homogenous matrix forming spermatodesmata. The conical acrosome is deeply invaginated posteriorly, and sits on top of the nucleus. The acrosomal contents are differentiated internally with a tubular substructure and a subacrosomal space. The anterior of the nucleus intrudes into the posterior section of the subacrosomal space. Anteriorly the acrosome is laterally flattened; posteriorly it extends as two tubular processes on either side of the nucleus that gradually decrease in diameter. The homogenously electron-dense nucleus is pointed anteriorly and is generally cylindrical, although posteriorly there is a lateral invagination that extends part-way along the nucleus. This invagination houses fine granular material of the putative centriolar adjunct which does not form in close proximity to the centriole and hence may not be a true centriolar adjunct. The lamellate disposition of the centriolar adjunct material within the sperm-midpiece of cicadettine cicadas is distinct, and separates these cicadas from their cicadine counterparts in which the centriolar adjunct material is non-lamellate. Vesicle-like elements that are associated with both the posterior nucleus and the centriolar adjunct are also found within the invagination. Immediately posterior of and adjoining the centriolar adjunct is a pair of mitochondrial derivatives that are elongated and extend for almost the entire length of the tail. Except for size the architecture of short and long spermatozoa is generally similar in all species. The absence of accessory bodies in cicada sperm suggests that within the Cicadomorpha, the families Cicadidae and Cercopidae are closely related. Only long nuclei were observed in the fertilized eggs of A. zuluensis indicating that sperm with long nuclei might be favoured for fertilization. Spermiogenesis involves: (a) development of the acrosome from a proacrosomal granule; (b) development of the nucleus, characterized by elongation and streamlining with a simultaneous condensation of chromatin; (c) development of the axoneme from the centriole; (d) amalgamation of individual small mitochondria to form elongated mitochondrial derivatives in which cristae are arranged into regularly spaced lamellae; and (f) elimination of cytoplasm. The presence of a manchette, a transient microtubular organelle, which surrounds the acrosome, nucleus and mitochondrial derivatives, is a characteristic feature of spermiogenesis. The gross morphology of the reproductive tract in both male and female cicadas exhibits an organization similar to that in most oviparous insects. The non-functional spermatheca is the only exceptional feature in the female reproductive tract. Its role has been taken over by the common oviduct which, subsequently, has become modified into a swollen, differentiated structure with a dual role of receiving oocytes from the paired ovaries and storage of spermatozoa. Testis mass varies between cicada species; this variation might be linked to the intensity of sperm competition which has been found to be positively correlated with relative investment in spermatogenesis. Based on the preliminary findings of this study, K. punctigera, with its larger testis relative to body size, would be the ideal candidate to show the greatest levels of sperm competition. Accessory glands in both male and female A. zuluensis, D. biconica, P. hirtipennis and O. quadraticollis are very long; this character might be of phylogenetic significance. Despite being notoriously refractory spermiocladistics is potentially valuable in systematic and phylogenic studies of cicadas, especially at the subfamily level.
- Full Text:
- Date Issued: 2007
- Authors: Chawanji, Abraham Simbarashe
- Date: 2007
- Subjects: Cicadas Cicadas -- Reproduction Spermiogenesis in animals
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5770 , http://hdl.handle.net/10962/d1005458
- Description: Sperm structure in five species of cicadine cicadas (Albanycada albigera, Azanicada zuluensis, Platypleura capensis, P. hirtipennis and Pycna semiclara) and five species of cicadettine cicadas (Melampsalta leucoptera, Quintilia walkeri, Stagira simplex, Xosopsaltria thunbergi and Monomatapa matoposa) was investigated by light and electron microscopy. In addition, spermiogenesis in cicadas was described; the information was derived from two cicadettines (Diceroprocta biconica and M. matoposa) and three cicadines (Kongota punctigera, P. capensis and P. semiclara). Mature spermatozoa of all species investigated are elongate and filiform, consisting of three distinct regions: the head (acrosome and nucleus), mid-piece and tail. All species produce more than one discrete length of nucleated, motile sperm, a form of sperm polymorphism termed polymegaly. Polymegaly is expressed in three ways; sperm have uni-, bi- or trimodal nucleus and tail lengths. Besides the differences in length, there are also notable differences in the size of nuclei. The anterior parts of sperm heads are embedded in an elongate homogenous matrix forming spermatodesmata. The conical acrosome is deeply invaginated posteriorly, and sits on top of the nucleus. The acrosomal contents are differentiated internally with a tubular substructure and a subacrosomal space. The anterior of the nucleus intrudes into the posterior section of the subacrosomal space. Anteriorly the acrosome is laterally flattened; posteriorly it extends as two tubular processes on either side of the nucleus that gradually decrease in diameter. The homogenously electron-dense nucleus is pointed anteriorly and is generally cylindrical, although posteriorly there is a lateral invagination that extends part-way along the nucleus. This invagination houses fine granular material of the putative centriolar adjunct which does not form in close proximity to the centriole and hence may not be a true centriolar adjunct. The lamellate disposition of the centriolar adjunct material within the sperm-midpiece of cicadettine cicadas is distinct, and separates these cicadas from their cicadine counterparts in which the centriolar adjunct material is non-lamellate. Vesicle-like elements that are associated with both the posterior nucleus and the centriolar adjunct are also found within the invagination. Immediately posterior of and adjoining the centriolar adjunct is a pair of mitochondrial derivatives that are elongated and extend for almost the entire length of the tail. Except for size the architecture of short and long spermatozoa is generally similar in all species. The absence of accessory bodies in cicada sperm suggests that within the Cicadomorpha, the families Cicadidae and Cercopidae are closely related. Only long nuclei were observed in the fertilized eggs of A. zuluensis indicating that sperm with long nuclei might be favoured for fertilization. Spermiogenesis involves: (a) development of the acrosome from a proacrosomal granule; (b) development of the nucleus, characterized by elongation and streamlining with a simultaneous condensation of chromatin; (c) development of the axoneme from the centriole; (d) amalgamation of individual small mitochondria to form elongated mitochondrial derivatives in which cristae are arranged into regularly spaced lamellae; and (f) elimination of cytoplasm. The presence of a manchette, a transient microtubular organelle, which surrounds the acrosome, nucleus and mitochondrial derivatives, is a characteristic feature of spermiogenesis. The gross morphology of the reproductive tract in both male and female cicadas exhibits an organization similar to that in most oviparous insects. The non-functional spermatheca is the only exceptional feature in the female reproductive tract. Its role has been taken over by the common oviduct which, subsequently, has become modified into a swollen, differentiated structure with a dual role of receiving oocytes from the paired ovaries and storage of spermatozoa. Testis mass varies between cicada species; this variation might be linked to the intensity of sperm competition which has been found to be positively correlated with relative investment in spermatogenesis. Based on the preliminary findings of this study, K. punctigera, with its larger testis relative to body size, would be the ideal candidate to show the greatest levels of sperm competition. Accessory glands in both male and female A. zuluensis, D. biconica, P. hirtipennis and O. quadraticollis are very long; this character might be of phylogenetic significance. Despite being notoriously refractory spermiocladistics is potentially valuable in systematic and phylogenic studies of cicadas, especially at the subfamily level.
- Full Text:
- Date Issued: 2007