Assembly of Omegatetravirus virus-like particles in the yeast Saccharomyces cerevisiae
- Authors: Tomasicchio, Michele
- Date: 2008
- Subjects: Helicoverpa armigera Imbrasia cytherea Viruses RNA viruses Insects -- Viruses Lepidoptera -- Viruses Saccharomyces cerevisiae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3930 , http://hdl.handle.net/10962/d1003989
- Description: The Tetraviridae are a family of ss (+) RNA viruses that specifically infect lepidopteran insects. Their icosahedral capsids are non-enveloped and approximately 40 nm in diameter with T=4 quasi-equivalent symmetry. The omegatetraviruses, which are structurally the best characterised in the family, include Helicoverpa armigera stunt virus (HaSV) and Nudaurelia capensis omega virus (NwV). The omegatetravirus procapsid is composed of 240 identical copies of the capsid precursor proteins, which undergo autoproteolytic cleavage at its carboxyl-terminus generating the mature capsid protein (b) and γ-peptide. This process occurs in vitro following a shift from pH 7.6 to pH 6.0. The viral capsid encapsidates two ss genomic RNAs: The larger RNA1 encodes the viral replicase as well as three small ORFs while RNA2 encodes the capsid precursor protein together with an overlapping ORF designated P17. While a wealth of structural data pertaining to the assembly and maturation of omegatetraviruses is available, little is known about how this relates to their lifecycle. The principle aim of the research described in this thesis was to use an experimental system developed in the yeast, Saccharomyces cerevisiae, to investigate the assembly of HaSV and NwV virus-like particles (VLPs) in terms of maturation and encapsidation of viral RNAs, in vivo. The yeast expression system used two promoter systems for expression of capsid precursor protein: in the first, a hybrid promoter (PGADH) was used for high-level expression, while the second, PGAL1, produced substantially lower levels of the virus capsid protein precursors. An increase in the level of HaSV capsid protein precursor (p71) via the PGADH promoter resulted in a dramatic increase in VLP assembly as compared with the PGAL system. A protein equivalent to the mature capsid protein (p64) appeared at later time intervals following induction of transcription. Transmission electron microscopic studies showed that p64 correlated with the presence of mature VLPs as opposed to procapsids in cells containing p71. This confirmed that the presence of p64 denoted maturation of VLPs in vivo. Further investigation indicated that maturation correlated with cell aging and the onset of apoptosis. It was shown that induction of apoptosis resulted in VLP maturation while inhibition of apoptosis prevented maturation. These results suggested that the process of apoptosis might be the trigger for maturation of virus procapsids in their host cells. The increase in the efficiency of VLP assembly observed in the high-level expression system was proposed to be due to an increase in the cellular concentrations of viral RNA. To test this hypothesis, HaSV P71 was co-expressed with either P71 mRNA or full length RNA2. An increase in the solubility of p71 was observed in cells expressing increased levels of both RNAs, but there was no increase in the efficiency of VLP assembly. Northern analysis of encapsidated RNAs revealed that there was no selective encapsidation of either P71 mRNA or viral RNA2. This data indicated that the increase in viral RNA was not the reason for increased efficiency of VLP assembly, but most likely resulted from higher concentrations of p71 itself. It was decided to determine whether a highly efficient nodavirus replication system developed in yeast for heterologous production of proteins, could be used as a method for expressing the capsid protein precursor. The aim of using this system was to determine if VLPs assembled in a replication system specifically encapsidated viral RNA. Transcripts encoding the NwV capsid protein precursor (p70) were generated in yeast cells by replication of a hybrid RNA template by the Nodamura virus (NoV) replicase. Western analysis confirmed the presence of p70 as well as a protein of 62 kDa corresponding to the mature NwV capsid protein. Northern analysis of purified VLPs showed that NoV RNA1 and RNA3 were encapsidated, but no RNA2 was detected. Taken together, the data lead to the conclusion that specific encapsidation of tetraviral RNAs required more than close proximity of the viral RNAs and assembling virus-like particles. Encapsidation specificity in the omegatetraviruses may require additional viral proteins such as p17 during encapsidation or specific viral RNA encapsidation was replication-dependent. Replication-dependent assembly has been shown in the nodaviruses.
- Full Text:
- Date Issued: 2008
- Authors: Tomasicchio, Michele
- Date: 2008
- Subjects: Helicoverpa armigera Imbrasia cytherea Viruses RNA viruses Insects -- Viruses Lepidoptera -- Viruses Saccharomyces cerevisiae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3930 , http://hdl.handle.net/10962/d1003989
- Description: The Tetraviridae are a family of ss (+) RNA viruses that specifically infect lepidopteran insects. Their icosahedral capsids are non-enveloped and approximately 40 nm in diameter with T=4 quasi-equivalent symmetry. The omegatetraviruses, which are structurally the best characterised in the family, include Helicoverpa armigera stunt virus (HaSV) and Nudaurelia capensis omega virus (NwV). The omegatetravirus procapsid is composed of 240 identical copies of the capsid precursor proteins, which undergo autoproteolytic cleavage at its carboxyl-terminus generating the mature capsid protein (b) and γ-peptide. This process occurs in vitro following a shift from pH 7.6 to pH 6.0. The viral capsid encapsidates two ss genomic RNAs: The larger RNA1 encodes the viral replicase as well as three small ORFs while RNA2 encodes the capsid precursor protein together with an overlapping ORF designated P17. While a wealth of structural data pertaining to the assembly and maturation of omegatetraviruses is available, little is known about how this relates to their lifecycle. The principle aim of the research described in this thesis was to use an experimental system developed in the yeast, Saccharomyces cerevisiae, to investigate the assembly of HaSV and NwV virus-like particles (VLPs) in terms of maturation and encapsidation of viral RNAs, in vivo. The yeast expression system used two promoter systems for expression of capsid precursor protein: in the first, a hybrid promoter (PGADH) was used for high-level expression, while the second, PGAL1, produced substantially lower levels of the virus capsid protein precursors. An increase in the level of HaSV capsid protein precursor (p71) via the PGADH promoter resulted in a dramatic increase in VLP assembly as compared with the PGAL system. A protein equivalent to the mature capsid protein (p64) appeared at later time intervals following induction of transcription. Transmission electron microscopic studies showed that p64 correlated with the presence of mature VLPs as opposed to procapsids in cells containing p71. This confirmed that the presence of p64 denoted maturation of VLPs in vivo. Further investigation indicated that maturation correlated with cell aging and the onset of apoptosis. It was shown that induction of apoptosis resulted in VLP maturation while inhibition of apoptosis prevented maturation. These results suggested that the process of apoptosis might be the trigger for maturation of virus procapsids in their host cells. The increase in the efficiency of VLP assembly observed in the high-level expression system was proposed to be due to an increase in the cellular concentrations of viral RNA. To test this hypothesis, HaSV P71 was co-expressed with either P71 mRNA or full length RNA2. An increase in the solubility of p71 was observed in cells expressing increased levels of both RNAs, but there was no increase in the efficiency of VLP assembly. Northern analysis of encapsidated RNAs revealed that there was no selective encapsidation of either P71 mRNA or viral RNA2. This data indicated that the increase in viral RNA was not the reason for increased efficiency of VLP assembly, but most likely resulted from higher concentrations of p71 itself. It was decided to determine whether a highly efficient nodavirus replication system developed in yeast for heterologous production of proteins, could be used as a method for expressing the capsid protein precursor. The aim of using this system was to determine if VLPs assembled in a replication system specifically encapsidated viral RNA. Transcripts encoding the NwV capsid protein precursor (p70) were generated in yeast cells by replication of a hybrid RNA template by the Nodamura virus (NoV) replicase. Western analysis confirmed the presence of p70 as well as a protein of 62 kDa corresponding to the mature NwV capsid protein. Northern analysis of purified VLPs showed that NoV RNA1 and RNA3 were encapsidated, but no RNA2 was detected. Taken together, the data lead to the conclusion that specific encapsidation of tetraviral RNAs required more than close proximity of the viral RNAs and assembling virus-like particles. Encapsidation specificity in the omegatetraviruses may require additional viral proteins such as p17 during encapsidation or specific viral RNA encapsidation was replication-dependent. Replication-dependent assembly has been shown in the nodaviruses.
- Full Text:
- Date Issued: 2008
ATP mimics as glutamine synthetase inhibitors : an exploratory synthetic study
- Authors: Salisu, Sheriff Tomilola
- Date: 2008
- Subjects: Glutamine synthetase Tuberculosis -- Treatment Tuberculosis -- Chemotherapy Adenosine triphosphate Adenosine triphosphate -- Synthesis Drug development
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4408 , http://hdl.handle.net/10962/d1006715
- Description: Using a mechanism-based approach to drug discovery, efforts have been directed towards developing novel ATP mimics that can act as GS inhibitors. The purine-based systems, adenosine, adenine and allopurinol, were identified as possible scaffolds for potential ATP mimics, while various meta-disubstituted benzenoid compounds, 3-aminobenzonitrile, 3-aminophenol, resorcinol, 3-aminobenzyl alcohol, 3-hydroxybenzoic acid and 3-aminobenzoic acid have been explored as adenine analogues. These compounds were treated with different alkylating and acylating agents. Allylation of all the substrates was achieved using allyl bromide and N-9 alkylation of protected allopurinol was effected using a number of specially prepared Baylis-Hillman adducts. Acylation of the benzenoid precursors with chloroacetyl chloride, acetoxyacetyl chloride, acryloyl chloride and specially prepared 2,3,4,5,6-pentaacetylgluconoyl chloride afforded the corresponding mono- and /or diacylated products in varying yields (4-96%). Elaboration of the alkylated and acylated products has involved the reaction of hydroxy systems with diethyl chloro phosphate and chloro derivatives with triethyl phosphite in Arbuzov-type reactions to afford phosphorylated products. In all cases, products were fully characterized using 1- and 2-D NMR analysis and, where appropriate, high-resolution mass spectrometry. The application of Modgraph and ChemWindow NMR prediction programmes has been explored and the resulting data have been compared with experimental chemical shift assignments to confirm chemical structures and, in some cases, to establish the position of allylation or acylation. Experimental assignments were found to be generally comparable with the Modgraph data, but not always with the ChemWindow values. The docking of selected products in the 'active-site' of GS and their structural homology with ATP, both in their free and bound conformations have been studied using the ACCELERYS Cerius² platform. All the selected ATP mimics exhibit some form of interaction with the 'active-site' residues, and a number of them appear to be promising GS ligands.
- Full Text:
- Date Issued: 2008
- Authors: Salisu, Sheriff Tomilola
- Date: 2008
- Subjects: Glutamine synthetase Tuberculosis -- Treatment Tuberculosis -- Chemotherapy Adenosine triphosphate Adenosine triphosphate -- Synthesis Drug development
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4408 , http://hdl.handle.net/10962/d1006715
- Description: Using a mechanism-based approach to drug discovery, efforts have been directed towards developing novel ATP mimics that can act as GS inhibitors. The purine-based systems, adenosine, adenine and allopurinol, were identified as possible scaffolds for potential ATP mimics, while various meta-disubstituted benzenoid compounds, 3-aminobenzonitrile, 3-aminophenol, resorcinol, 3-aminobenzyl alcohol, 3-hydroxybenzoic acid and 3-aminobenzoic acid have been explored as adenine analogues. These compounds were treated with different alkylating and acylating agents. Allylation of all the substrates was achieved using allyl bromide and N-9 alkylation of protected allopurinol was effected using a number of specially prepared Baylis-Hillman adducts. Acylation of the benzenoid precursors with chloroacetyl chloride, acetoxyacetyl chloride, acryloyl chloride and specially prepared 2,3,4,5,6-pentaacetylgluconoyl chloride afforded the corresponding mono- and /or diacylated products in varying yields (4-96%). Elaboration of the alkylated and acylated products has involved the reaction of hydroxy systems with diethyl chloro phosphate and chloro derivatives with triethyl phosphite in Arbuzov-type reactions to afford phosphorylated products. In all cases, products were fully characterized using 1- and 2-D NMR analysis and, where appropriate, high-resolution mass spectrometry. The application of Modgraph and ChemWindow NMR prediction programmes has been explored and the resulting data have been compared with experimental chemical shift assignments to confirm chemical structures and, in some cases, to establish the position of allylation or acylation. Experimental assignments were found to be generally comparable with the Modgraph data, but not always with the ChemWindow values. The docking of selected products in the 'active-site' of GS and their structural homology with ATP, both in their free and bound conformations have been studied using the ACCELERYS Cerius² platform. All the selected ATP mimics exhibit some form of interaction with the 'active-site' residues, and a number of them appear to be promising GS ligands.
- Full Text:
- Date Issued: 2008
Bioprocess development for removal of nitrogenous compounds from precious metal refinery wastewater
- Manipura, Walappuly Mudiyanselage Janakasiri Aruna Shantha Bandara
- Authors: Manipura, Walappuly Mudiyanselage Janakasiri Aruna Shantha Bandara
- Date: 2008
- Subjects: Factory and trade waste Centralized industrial waste treatment facilities Metals -- Absorption and adsorption Metals -- Environmental aspects Water -- Purification -- Mathematical models Water quality management Water reuse Metals -- Refining Microbiology -- Research
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4076 , http://hdl.handle.net/10962/d1007341
- Description: Removal of nitrogenous compounds from precious metal refinery (PMR) wastewater is important in terms of avoiding eutrophication (environmental protection), metal recovery (increased overall process efficiency and value recovery) and reuse of treated water (maximum use of natural resources). Extreme pH conditions (4 to 13 depending on the wastewater stream), high chemical oxygen demand (> 10,000 mg/I), numerous metals and high concentrations of those metals (> 20 mg/l of platinum group metals) in the wastewater are the main challenges for biological removal of nitrogenous compounds from PMR wastewater. Nitrogenous compounds such as NH₄⁺-N and N0₃-N are strong metal ligands, which make it difficult to recover metals from the wastewater. Therefore, a bioprocess was developed for removal of nitrogenous compounds from carefully simulated PMR wastewater. A preliminary investigation of metal wastewater was carried out to determine its composition and physico-chemical properties, the ability to nitrify and denitrify under different pH conditions and denitrification with different carbon Source compounds and amounts. Even at pH 4, nitrification could be carried out. A suitable hydraulic retention time was found to be 72 hours. There was no significant difference between sodium acetate and sodium lactate as carbon sources for denitrification. Based on these results, a reactor comparison study was carried out using simulated PMR wastewater in three types of reactors: continuously stirred tank reactor (CSTR), packed-bed reactor (PBR) and airlift suspension reactor (ALSR). These reactors were fed with 30 mg/l of Rh bound in an NH₄⁺ based compound (Claus salt: pentaaminechlororhodium (III) dichloride). Total nitrogen removal efficiencies of > 68 % , > 79 % and > 45 % were obtained in the CSTR, PBR and ALSR, respectively. Serially connected CSTR-PBR and PBR-CSTR reactor configurations were then studied to determine the best configuration for maximum removal of nitrogenous compounds from the wastewater. The PBR-CSTR configuration gave consistent biomass retention and automatic pH control in the CSTR. Ammonium removal efficiencies > 95 % were achieved in both reactors. As poor nitrate removal was observed a toxicity study was carried out using respirometry and the half saturation inhibition coefficients for Pt, Pd, Rh and Ru were found to be 15.81, 25.00, 33.34 and 39.25 mg/l, respectively. A mathematical model was developed to describe the nitrogen removal in PMR wastewater using activated sludge model number 1 (ASMl), two step nitrification and metal toxicity. An operational protocol was developed based on the literature review, experimental work and simulation results. The optimum reactor configuration under the set conditions (20 mg/I of Rh and < 100 mg/I of NH₄⁺-N) was found to be PBR-CSTR-PBR process, which achieved overall NH₄⁺-N and N0₃⁻-N removal efficiencies of > 90 % and 95 %, respectively. Finally, a rudimentary microbial characterisation was carried out on subsamples from the CSTR and PBRsecondary. It was found that the CSTR biomass consisted of both rods and cocci while PBRsecondary consisted of rods only. Based on these experimental works, further research needs and recommendations were made for optimisation of the developed bioprocess for removal of nitrogenous compounds from PMR wastewater.
- Full Text:
- Date Issued: 2008
- Authors: Manipura, Walappuly Mudiyanselage Janakasiri Aruna Shantha Bandara
- Date: 2008
- Subjects: Factory and trade waste Centralized industrial waste treatment facilities Metals -- Absorption and adsorption Metals -- Environmental aspects Water -- Purification -- Mathematical models Water quality management Water reuse Metals -- Refining Microbiology -- Research
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4076 , http://hdl.handle.net/10962/d1007341
- Description: Removal of nitrogenous compounds from precious metal refinery (PMR) wastewater is important in terms of avoiding eutrophication (environmental protection), metal recovery (increased overall process efficiency and value recovery) and reuse of treated water (maximum use of natural resources). Extreme pH conditions (4 to 13 depending on the wastewater stream), high chemical oxygen demand (> 10,000 mg/I), numerous metals and high concentrations of those metals (> 20 mg/l of platinum group metals) in the wastewater are the main challenges for biological removal of nitrogenous compounds from PMR wastewater. Nitrogenous compounds such as NH₄⁺-N and N0₃-N are strong metal ligands, which make it difficult to recover metals from the wastewater. Therefore, a bioprocess was developed for removal of nitrogenous compounds from carefully simulated PMR wastewater. A preliminary investigation of metal wastewater was carried out to determine its composition and physico-chemical properties, the ability to nitrify and denitrify under different pH conditions and denitrification with different carbon Source compounds and amounts. Even at pH 4, nitrification could be carried out. A suitable hydraulic retention time was found to be 72 hours. There was no significant difference between sodium acetate and sodium lactate as carbon sources for denitrification. Based on these results, a reactor comparison study was carried out using simulated PMR wastewater in three types of reactors: continuously stirred tank reactor (CSTR), packed-bed reactor (PBR) and airlift suspension reactor (ALSR). These reactors were fed with 30 mg/l of Rh bound in an NH₄⁺ based compound (Claus salt: pentaaminechlororhodium (III) dichloride). Total nitrogen removal efficiencies of > 68 % , > 79 % and > 45 % were obtained in the CSTR, PBR and ALSR, respectively. Serially connected CSTR-PBR and PBR-CSTR reactor configurations were then studied to determine the best configuration for maximum removal of nitrogenous compounds from the wastewater. The PBR-CSTR configuration gave consistent biomass retention and automatic pH control in the CSTR. Ammonium removal efficiencies > 95 % were achieved in both reactors. As poor nitrate removal was observed a toxicity study was carried out using respirometry and the half saturation inhibition coefficients for Pt, Pd, Rh and Ru were found to be 15.81, 25.00, 33.34 and 39.25 mg/l, respectively. A mathematical model was developed to describe the nitrogen removal in PMR wastewater using activated sludge model number 1 (ASMl), two step nitrification and metal toxicity. An operational protocol was developed based on the literature review, experimental work and simulation results. The optimum reactor configuration under the set conditions (20 mg/I of Rh and < 100 mg/I of NH₄⁺-N) was found to be PBR-CSTR-PBR process, which achieved overall NH₄⁺-N and N0₃⁻-N removal efficiencies of > 90 % and 95 %, respectively. Finally, a rudimentary microbial characterisation was carried out on subsamples from the CSTR and PBRsecondary. It was found that the CSTR biomass consisted of both rods and cocci while PBRsecondary consisted of rods only. Based on these experimental works, further research needs and recommendations were made for optimisation of the developed bioprocess for removal of nitrogenous compounds from PMR wastewater.
- Full Text:
- Date Issued: 2008
Biosorption of precious metals from synthetic and refinery wastewaters by immobilized saccharomyces cerevisiae
- Authors: Mack, Cherie-Lynn
- Date: 2008
- Subjects: Metals -- Refining Metals -- Absorption and adsorption Saccharomyces cerevisiae Factory and trade waste Water reuse Platinum
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4071 , http://hdl.handle.net/10962/d1006977
- Description: The process of precious metal refining can be up to 99.99% efficient at best, and although it may seem small, the amount of valuable metal lost to waste streams is appreciable enough to warrant recovery. The method currently used to remove entrained metal ions from refinery wastewaters, chemical precipitation, is not an effective means for selective recovery of precious metals from a wastewater. Biosorption, the ability of certain types of biomass to bind and concentrate metals from even very dilute aqueous solutions, may be an effective point-source metal recovery strategy. The yeast, Saccharomyces cerevisiae, has been found capable of sorbing numerous precious and base metals, and is a cheap and abundant source of biomass. As such, it represents a possible precious metal sorbent for application to refining wastewaters. In this investigation, S. cerevisiae biomass was immobilized, using polyethyleneimine and glutaraldehyde, to produce a suitable sorbent, which was found to be capable of high platinum uptake (150 to 170 mg/g) at low pH (< 2). The sorption mechanism was elucidated and found to be a chemical reaction, which made effective desorption impossible. The sorption process was investigated in a packed bed column conformation, the results of which showed that the diameter and height of the column require further optimization in order to attain the metal uptake values achieved in the batch studies. When applied to a refinery wastewater, two key wastewater characteristics limited the success of the sorption process; the high inorganic ion content and the complex speciation of the platinum ions. The results proved the concept principle of platinum recovery by immobilized yeast biosorption and indicated that a more detailed understanding of the platinum speciation within the wastewater is required before the biosorption process can be applied. Overall, the sorption of platinum by the S. cerevisiae sorbent was demonstrated to be highly effective in principle, but the complexity of the wastewater requires that pretreatment steps be taken before the successful application of this process to an industrial wastewater.
- Full Text:
- Date Issued: 2008
- Authors: Mack, Cherie-Lynn
- Date: 2008
- Subjects: Metals -- Refining Metals -- Absorption and adsorption Saccharomyces cerevisiae Factory and trade waste Water reuse Platinum
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4071 , http://hdl.handle.net/10962/d1006977
- Description: The process of precious metal refining can be up to 99.99% efficient at best, and although it may seem small, the amount of valuable metal lost to waste streams is appreciable enough to warrant recovery. The method currently used to remove entrained metal ions from refinery wastewaters, chemical precipitation, is not an effective means for selective recovery of precious metals from a wastewater. Biosorption, the ability of certain types of biomass to bind and concentrate metals from even very dilute aqueous solutions, may be an effective point-source metal recovery strategy. The yeast, Saccharomyces cerevisiae, has been found capable of sorbing numerous precious and base metals, and is a cheap and abundant source of biomass. As such, it represents a possible precious metal sorbent for application to refining wastewaters. In this investigation, S. cerevisiae biomass was immobilized, using polyethyleneimine and glutaraldehyde, to produce a suitable sorbent, which was found to be capable of high platinum uptake (150 to 170 mg/g) at low pH (< 2). The sorption mechanism was elucidated and found to be a chemical reaction, which made effective desorption impossible. The sorption process was investigated in a packed bed column conformation, the results of which showed that the diameter and height of the column require further optimization in order to attain the metal uptake values achieved in the batch studies. When applied to a refinery wastewater, two key wastewater characteristics limited the success of the sorption process; the high inorganic ion content and the complex speciation of the platinum ions. The results proved the concept principle of platinum recovery by immobilized yeast biosorption and indicated that a more detailed understanding of the platinum speciation within the wastewater is required before the biosorption process can be applied. Overall, the sorption of platinum by the S. cerevisiae sorbent was demonstrated to be highly effective in principle, but the complexity of the wastewater requires that pretreatment steps be taken before the successful application of this process to an industrial wastewater.
- Full Text:
- Date Issued: 2008
Black parental involvement in education
- Authors: Mbokodi, Sindiswa Madgie
- Date: 2008
- Subjects: Education -- Parent participation -- South Africa , Parent-teacher relationships -- South Africa
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:9518 , http://hdl.handle.net/10948/1013 , Education -- Parent participation -- South Africa , Parent-teacher relationships -- South Africa
- Description: The study was conducted in the backdrop of Section 24(1)(a) of the South African Schools Act (SASA) 84 of 1996 which promulgated that parents should be involved in the education of their children. To fulfil this requirement, each school is expected to have a legally constituted School Governing Body (SGB) as part of school governance. The involvement of the SGB does not exclude parents involving themselves in school activities as individuals but as organised groups geared towards working on a specific project of the school. Involvement also implies helping the child at home. The study investigated the extent to which black parents involve themselves in school activities as envisaged by the SASA. Conceptual literature relating to issues of parental involvement in school was reviewed. Specifically the concept parent/parental involvement as it relates to black parents was explored. Factors promoting or hindering parental involvement in school were also examined. The research is qualitative. Participants included principals, teachers, learners and chairpersons of school governing bodies of ten selected schools in the major townships of Port Elizabeth. Views and experiences of participants were captured and explained through in-depth interviews and observations. Results are discussed in relation to the relevant literature, allowing grounded theory to emerge. What emerged was a desperate situation of non-involvement of black parents in school activities largely because of the failure of the Department of Education (DoE) to capacitate all parties involved in strategies to involve parents in school. The potential for generalizability and transferability is discussed in the report and possible intervention strategies are recommended at micro- and macro-levels. The study demonstrates that the DoE needs to follow up on its policies to ensure that the envisaged change is realized.
- Full Text:
- Date Issued: 2008
- Authors: Mbokodi, Sindiswa Madgie
- Date: 2008
- Subjects: Education -- Parent participation -- South Africa , Parent-teacher relationships -- South Africa
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:9518 , http://hdl.handle.net/10948/1013 , Education -- Parent participation -- South Africa , Parent-teacher relationships -- South Africa
- Description: The study was conducted in the backdrop of Section 24(1)(a) of the South African Schools Act (SASA) 84 of 1996 which promulgated that parents should be involved in the education of their children. To fulfil this requirement, each school is expected to have a legally constituted School Governing Body (SGB) as part of school governance. The involvement of the SGB does not exclude parents involving themselves in school activities as individuals but as organised groups geared towards working on a specific project of the school. Involvement also implies helping the child at home. The study investigated the extent to which black parents involve themselves in school activities as envisaged by the SASA. Conceptual literature relating to issues of parental involvement in school was reviewed. Specifically the concept parent/parental involvement as it relates to black parents was explored. Factors promoting or hindering parental involvement in school were also examined. The research is qualitative. Participants included principals, teachers, learners and chairpersons of school governing bodies of ten selected schools in the major townships of Port Elizabeth. Views and experiences of participants were captured and explained through in-depth interviews and observations. Results are discussed in relation to the relevant literature, allowing grounded theory to emerge. What emerged was a desperate situation of non-involvement of black parents in school activities largely because of the failure of the Department of Education (DoE) to capacitate all parties involved in strategies to involve parents in school. The potential for generalizability and transferability is discussed in the report and possible intervention strategies are recommended at micro- and macro-levels. The study demonstrates that the DoE needs to follow up on its policies to ensure that the envisaged change is realized.
- Full Text:
- Date Issued: 2008
Camphor derivatives in asymmetric synthesis: a synthetic, mechanistic and theoretical study
- Authors: Lobb, Kevin Alan
- Date: 2008
- Subjects: Chemistry, Organic -- Research Esters Organic compounds -- Synthesis Alkylation Chemical reactions -- Computer simulation Chemical kinetics Camphor Cinnamomum
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4414 , http://hdl.handle.net/10962/d1006770
- Description: A series of 3,3-ethylenedioxy-exo- and endo- bornyl esters have been prepared and subjected to α-benzylation using lithium diisopropylamide and benzyl bromide. In the exo-series of esters the diastereofacial selectivity of benzylation was found to improve (up to 34% d.e.) as the steric bulk of the O-alkyl group increased, whereas in the endo-series, a surprising decrease in stereoselectivity was observed as the steric bulk increased – an observation attributed to flexibility of the metal-coordinated endo-enolate system, compared to the relative rigidity of the exo analogues. The conformational options for each series was explored at the density functional theory level. Reductive cyclization of a range of specially prepared N-carbobenzyloxy-amino acid esters has been shown to afford the corresponding derivatives, contrary to previous reports that the cyclization is limited to the glycine derivative. The cyclization sequence has been explored in detail, and the yield has been shown to be critically dependent on the stereochemistry of the α-amino acid moiety. Moreover, it seems that reductive cyclization occurs more readily with the endo- rather than the exo-bornyl N-CBZ-amino acid esters. Molecular modelling of relevant transition states at the DFT levels indicates that L-amino acid-derived systems should cyclize preferably in the exo-series and D-amino acid-derived systems should cyclize preferably in the endo series. Studies of alkylation of an iminolactone system have reported an interesting anomaly - exo-methylation is observed while endo-alkylation predominates for larger alkyl groups. This has been studied in detail at the DFT level, and the anomaly is attributed to thermodynamic control in the methyl case, whereas kinetic control is the norm in this system. Preliminary computer modelling of the intramolecular rearrangement of a 3,3-xylylbornyl system at the HF/STO-3G level raised doubts concerning the structure assigned by Evans to one of the rearrangement products, prompting an X-ray crystallographic analysis and leading to the revision of its structure from a pinene to a camphene derivative. The previously elusive spiro[bornane-3,2’-indan]-2-exo-tosylate has been successfully isolated, and the kinetics of its ready decomposition to the two camphene products has been followed by 1H NMR spectroscopy. The endo-tosylate analogue, on the other hand, was found to be remarkably stable. Kinetic data obtained for rearrangement of this exo-bornyl tosylate have indicated the operation of tandem autocatalytic and pseudo-first-order transformations leading sequentially to the two isomeric camphene products. An extensive coset analysis of all possible rearrangement processes of the initially-formed cation formed from decomposition of the exo-tosylate has afforded a graph containing 336 classical cations (modelled at the AM1 and B3LYP levels) and 526 transition-state complexes (modelled at the AM1 level). This analysis afforded a viable 4-step classical mechanism connecting the first camphene product with the second. A more realistic study, involving non-classical carbocations, has afforded a graph of all possible (classical and non-classical) cations that could be formed by rearrangment of the initiallyformed cation. The resulting graph confirms that the only energetically feasible path corresponds to the classical mechanism, but simply involves two steps, including a novel, concerted Wagner-Meerwein – 6,2-hydride shift – Wagner-Meerwein rearrangement.
- Full Text:
- Date Issued: 2008
- Authors: Lobb, Kevin Alan
- Date: 2008
- Subjects: Chemistry, Organic -- Research Esters Organic compounds -- Synthesis Alkylation Chemical reactions -- Computer simulation Chemical kinetics Camphor Cinnamomum
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4414 , http://hdl.handle.net/10962/d1006770
- Description: A series of 3,3-ethylenedioxy-exo- and endo- bornyl esters have been prepared and subjected to α-benzylation using lithium diisopropylamide and benzyl bromide. In the exo-series of esters the diastereofacial selectivity of benzylation was found to improve (up to 34% d.e.) as the steric bulk of the O-alkyl group increased, whereas in the endo-series, a surprising decrease in stereoselectivity was observed as the steric bulk increased – an observation attributed to flexibility of the metal-coordinated endo-enolate system, compared to the relative rigidity of the exo analogues. The conformational options for each series was explored at the density functional theory level. Reductive cyclization of a range of specially prepared N-carbobenzyloxy-amino acid esters has been shown to afford the corresponding derivatives, contrary to previous reports that the cyclization is limited to the glycine derivative. The cyclization sequence has been explored in detail, and the yield has been shown to be critically dependent on the stereochemistry of the α-amino acid moiety. Moreover, it seems that reductive cyclization occurs more readily with the endo- rather than the exo-bornyl N-CBZ-amino acid esters. Molecular modelling of relevant transition states at the DFT levels indicates that L-amino acid-derived systems should cyclize preferably in the exo-series and D-amino acid-derived systems should cyclize preferably in the endo series. Studies of alkylation of an iminolactone system have reported an interesting anomaly - exo-methylation is observed while endo-alkylation predominates for larger alkyl groups. This has been studied in detail at the DFT level, and the anomaly is attributed to thermodynamic control in the methyl case, whereas kinetic control is the norm in this system. Preliminary computer modelling of the intramolecular rearrangement of a 3,3-xylylbornyl system at the HF/STO-3G level raised doubts concerning the structure assigned by Evans to one of the rearrangement products, prompting an X-ray crystallographic analysis and leading to the revision of its structure from a pinene to a camphene derivative. The previously elusive spiro[bornane-3,2’-indan]-2-exo-tosylate has been successfully isolated, and the kinetics of its ready decomposition to the two camphene products has been followed by 1H NMR spectroscopy. The endo-tosylate analogue, on the other hand, was found to be remarkably stable. Kinetic data obtained for rearrangement of this exo-bornyl tosylate have indicated the operation of tandem autocatalytic and pseudo-first-order transformations leading sequentially to the two isomeric camphene products. An extensive coset analysis of all possible rearrangement processes of the initially-formed cation formed from decomposition of the exo-tosylate has afforded a graph containing 336 classical cations (modelled at the AM1 and B3LYP levels) and 526 transition-state complexes (modelled at the AM1 level). This analysis afforded a viable 4-step classical mechanism connecting the first camphene product with the second. A more realistic study, involving non-classical carbocations, has afforded a graph of all possible (classical and non-classical) cations that could be formed by rearrangment of the initiallyformed cation. The resulting graph confirms that the only energetically feasible path corresponds to the classical mechanism, but simply involves two steps, including a novel, concerted Wagner-Meerwein – 6,2-hydride shift – Wagner-Meerwein rearrangement.
- Full Text:
- Date Issued: 2008
Changing words and worlds?: a phenomenological study of the acquisition of an academic literacy
- Authors: Thomson, Carol Irene
- Date: 2008
- Subjects: Education -- Study and teaching (Higher) -- South Africa Universities and colleges -- South Africa College student development programs -- South Africa Literacy -- South Africa Education, Higher -- Philosophy Educational change -- South Africa Phenomenology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1446 , http://hdl.handle.net/10962/d1003327
- Description: This study is contextualised within the field of post-graduate, continuing teacher education, and the vibrant and demanding policy context that has characterised higher education in post-apartheid South Africa. Situated within a module specifically designed to address what is commonly understood to be the academic literacy development needs of students in the Bachelor of Education Honours programme at the former University of Natal, it aims to unveil the lived experiences of students taking this module. The module, Reading and Writing Academic Texts (RWAT), was developed in direct response to academics’ call that something be done about the ‘problem’ of students’ reading and writing proficiency. As a core, compulsory module, RWAT was informed by Systemic Functional Linguistics and drew on Genre Theory for its conceptual and theoretical framework. It foregrounded the genre of the academic argument as the key academic literacy that was taught. The motivation for this study came from my own increasing concern that the theoretical and conceptual framework we had adopted for the module was emerging as an inherently limiting and formulaic model of literacy, and was resulting in students exiting the module with little or no ‘critical’ perspective on any aspect of literacy as social practice. I was also keen, in a climate of increasing de-personalisation and the massification of education, to reinstate the personal. Thus, I chose to focus on individual lives, and through an exploration of a small group of participants’ ‘lived’ experiences of the RWAT module, ascertain what it is like to acquire an academic literacy. The key research question is, therefore: What is it like to acquire an academic literacy? The secondary research question is: How is this experience influenced by the mode of delivery in which it occurs? For its conceptual and theoretical framing, this study draws on social literacy theory and phenomenology, the latter as both a philosophy and a methodology. However, although the study has drawn significantly on the phenomenological tradition for inspiration and direction, it has not done so uncritically. Thus, the study engages with phenomenology-as-philosophy in great depth before turning to phenomenology-as-methodology, in order to arrive at a point where the methods and procedures applied in it, are justified. The main findings of the study suggest that, despite the RWAT module espousing an ideological model (Street, 1984) of literacy in its learning materials and readings, participants came very much closer to experiencing an autonomous model of literacy (Street, 1984). The data shows that the RWAT module was largely inadequate to the task of inducting participants into the ‘situated practices’ and ‘situated meanings’ of the Discourse of Genre Theory and/or the academy, hence the many ‘lived’ difficulties participants experienced. The data also highlights the ease with which an autonomous model of literacy can come to govern practice and student experience even when curriculum intention is underpinned by an ideological position on literacy as social practice. Finally, the study suggests that the research community in South Africa, characterised as it is by such diversity, would be enriched by more studies derived from phenomenology, and a continuing engagement with phenomenology-as-a-movement in order to both challenge and expand its existing framework.
- Full Text:
- Date Issued: 2008
- Authors: Thomson, Carol Irene
- Date: 2008
- Subjects: Education -- Study and teaching (Higher) -- South Africa Universities and colleges -- South Africa College student development programs -- South Africa Literacy -- South Africa Education, Higher -- Philosophy Educational change -- South Africa Phenomenology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1446 , http://hdl.handle.net/10962/d1003327
- Description: This study is contextualised within the field of post-graduate, continuing teacher education, and the vibrant and demanding policy context that has characterised higher education in post-apartheid South Africa. Situated within a module specifically designed to address what is commonly understood to be the academic literacy development needs of students in the Bachelor of Education Honours programme at the former University of Natal, it aims to unveil the lived experiences of students taking this module. The module, Reading and Writing Academic Texts (RWAT), was developed in direct response to academics’ call that something be done about the ‘problem’ of students’ reading and writing proficiency. As a core, compulsory module, RWAT was informed by Systemic Functional Linguistics and drew on Genre Theory for its conceptual and theoretical framework. It foregrounded the genre of the academic argument as the key academic literacy that was taught. The motivation for this study came from my own increasing concern that the theoretical and conceptual framework we had adopted for the module was emerging as an inherently limiting and formulaic model of literacy, and was resulting in students exiting the module with little or no ‘critical’ perspective on any aspect of literacy as social practice. I was also keen, in a climate of increasing de-personalisation and the massification of education, to reinstate the personal. Thus, I chose to focus on individual lives, and through an exploration of a small group of participants’ ‘lived’ experiences of the RWAT module, ascertain what it is like to acquire an academic literacy. The key research question is, therefore: What is it like to acquire an academic literacy? The secondary research question is: How is this experience influenced by the mode of delivery in which it occurs? For its conceptual and theoretical framing, this study draws on social literacy theory and phenomenology, the latter as both a philosophy and a methodology. However, although the study has drawn significantly on the phenomenological tradition for inspiration and direction, it has not done so uncritically. Thus, the study engages with phenomenology-as-philosophy in great depth before turning to phenomenology-as-methodology, in order to arrive at a point where the methods and procedures applied in it, are justified. The main findings of the study suggest that, despite the RWAT module espousing an ideological model (Street, 1984) of literacy in its learning materials and readings, participants came very much closer to experiencing an autonomous model of literacy (Street, 1984). The data shows that the RWAT module was largely inadequate to the task of inducting participants into the ‘situated practices’ and ‘situated meanings’ of the Discourse of Genre Theory and/or the academy, hence the many ‘lived’ difficulties participants experienced. The data also highlights the ease with which an autonomous model of literacy can come to govern practice and student experience even when curriculum intention is underpinned by an ideological position on literacy as social practice. Finally, the study suggests that the research community in South Africa, characterised as it is by such diversity, would be enriched by more studies derived from phenomenology, and a continuing engagement with phenomenology-as-a-movement in order to both challenge and expand its existing framework.
- Full Text:
- Date Issued: 2008
Communal production systems of goats raised by resource-poor farmers in the Eastern Cape Province of South Africa
- Authors: Gwaze, Francisca Rumosa
- Date: 2008
- Subjects: Agriculture -- Economic aspects -- South Africa -- Eastern Cape , Commons -- South Africa -- Eastern Cape , Pastoral systems -- Economic aspects -- South Africa -- Eastern Cape , Goats -- Economic aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD (Animal Science)
- Identifier: vital:11578 , http://hdl.handle.net/10353/172 , Agriculture -- Economic aspects -- South Africa -- Eastern Cape , Commons -- South Africa -- Eastern Cape , Pastoral systems -- Economic aspects -- South Africa -- Eastern Cape , Goats -- Economic aspects -- South Africa -- Eastern Cape
- Description: Goats significantly contribute towards the subsistence, economic and social livelihoods of many resource-poor farmers in developing countries, the majority of which own goats. There, however, is dearth of information on communal goat production potential and roles to the rural populace. The broad objective of the study was to evaluate production practices, constraints, production efficiency and to determine nutritional and health status of goats raised by resource-poor communal farmers in the Eastern Cape Province of South Africa. Roles and management systems of goats, goat flock dynamics and, prevalence and loads of gastrointestinal infections and the nutritional status of goats in the study areas were determined. The relationships among body weight, body condition score, faecal egg counts and, haematological and biochemical profiles were also determined. Mean goat flock sizes per household were similar between the two districts studied; Amatole (14.0 ± 0.31) and Alfred Nzo (14.1 ± 1.42). Seventy-nine percent of households in Amatole and 78% in Alfred Nzo kept goats for ceremonies, such as the initiation ceremonies. Goat houses in the two districts were poorly constructed. Thirty two percent of farmers in Alfred Nzo district and 27% in Amatole district reported low buck to doe ratios, suggesting that inbreeding might have been reducing productivity of their flocks. Kid mortality had two major peaks; in May (21%) and in September (21%). Goat production potential (GPP), the proportion of mature and growing goats to the total flock size, was affected by gender of owner of goats. Goats owned by female had a significantly lower GPP value of 0.63 ± 0.015 than goats owned by male farmers (0.70 ± 0.010). Month also significantly affected GPP with the highest (P < 0.05) GPP recorded in May, June and July and the lowest in March and April. Village affected GPP with values for Nkosana and Qawukeni being significantly higher than for Mankone. Goat production potential was also higher (P < 0.05) in small flocks (0.04 ± 0.008) than in large flocks (0.02 ± 0.008). Goat production efficiency (GPE) ranged from 0.11 ± 0.193 in April to 1.55 ± 0.193 in December. The most prevalent gastrointestinal eggs were the strongyle egg type (68.4 ± 8.49 in Qawukeni and 96.1 ± 12.01 in Nkosana) followed by coccidia (53.3 ± 8.76 in Qawukeni and 68.8 ± 8.00 in Mankone). The other identified nematodes were Strongyloides and Trichostrongylus egg types. The trematodes observed were Fasciola and Paramphistomum species. High loads of strongyle eggs were observed in the hot-wet season and the post-rainy season, whilst the other egg types showed a peak in the hot-wet season only. For most of the gastrointestinal parasite eggs, prevalence was higher (P < 0.05) in the sour rangeland compared to the sweet rangeland. Higher (P < 0.05) levels of total protein (TP), globulin, aspartate aminotransferase (AST) and creatinine kinase (CK) levels were recorded in the wet than in the dry season. Body condition scores were positively correlated (P < 0.05) to albumin, body weight and packed cell volume. However, body condition scores were negatively correlated to TP, glucose, alanine transaminase (ALT) and AST. Strongyle egg loads were positively correlated to FAMACHA scores, packed cell volume, body weight and body condition score. The observed high globulin levels suggested a chronic health challenge. Thus, it is fundamental to devise affordable interventions for the control of gastrointestinal parasites in communal goats leading to improved goat productivity and hence rural livelihood
- Full Text:
- Date Issued: 2008
- Authors: Gwaze, Francisca Rumosa
- Date: 2008
- Subjects: Agriculture -- Economic aspects -- South Africa -- Eastern Cape , Commons -- South Africa -- Eastern Cape , Pastoral systems -- Economic aspects -- South Africa -- Eastern Cape , Goats -- Economic aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD (Animal Science)
- Identifier: vital:11578 , http://hdl.handle.net/10353/172 , Agriculture -- Economic aspects -- South Africa -- Eastern Cape , Commons -- South Africa -- Eastern Cape , Pastoral systems -- Economic aspects -- South Africa -- Eastern Cape , Goats -- Economic aspects -- South Africa -- Eastern Cape
- Description: Goats significantly contribute towards the subsistence, economic and social livelihoods of many resource-poor farmers in developing countries, the majority of which own goats. There, however, is dearth of information on communal goat production potential and roles to the rural populace. The broad objective of the study was to evaluate production practices, constraints, production efficiency and to determine nutritional and health status of goats raised by resource-poor communal farmers in the Eastern Cape Province of South Africa. Roles and management systems of goats, goat flock dynamics and, prevalence and loads of gastrointestinal infections and the nutritional status of goats in the study areas were determined. The relationships among body weight, body condition score, faecal egg counts and, haematological and biochemical profiles were also determined. Mean goat flock sizes per household were similar between the two districts studied; Amatole (14.0 ± 0.31) and Alfred Nzo (14.1 ± 1.42). Seventy-nine percent of households in Amatole and 78% in Alfred Nzo kept goats for ceremonies, such as the initiation ceremonies. Goat houses in the two districts were poorly constructed. Thirty two percent of farmers in Alfred Nzo district and 27% in Amatole district reported low buck to doe ratios, suggesting that inbreeding might have been reducing productivity of their flocks. Kid mortality had two major peaks; in May (21%) and in September (21%). Goat production potential (GPP), the proportion of mature and growing goats to the total flock size, was affected by gender of owner of goats. Goats owned by female had a significantly lower GPP value of 0.63 ± 0.015 than goats owned by male farmers (0.70 ± 0.010). Month also significantly affected GPP with the highest (P < 0.05) GPP recorded in May, June and July and the lowest in March and April. Village affected GPP with values for Nkosana and Qawukeni being significantly higher than for Mankone. Goat production potential was also higher (P < 0.05) in small flocks (0.04 ± 0.008) than in large flocks (0.02 ± 0.008). Goat production efficiency (GPE) ranged from 0.11 ± 0.193 in April to 1.55 ± 0.193 in December. The most prevalent gastrointestinal eggs were the strongyle egg type (68.4 ± 8.49 in Qawukeni and 96.1 ± 12.01 in Nkosana) followed by coccidia (53.3 ± 8.76 in Qawukeni and 68.8 ± 8.00 in Mankone). The other identified nematodes were Strongyloides and Trichostrongylus egg types. The trematodes observed were Fasciola and Paramphistomum species. High loads of strongyle eggs were observed in the hot-wet season and the post-rainy season, whilst the other egg types showed a peak in the hot-wet season only. For most of the gastrointestinal parasite eggs, prevalence was higher (P < 0.05) in the sour rangeland compared to the sweet rangeland. Higher (P < 0.05) levels of total protein (TP), globulin, aspartate aminotransferase (AST) and creatinine kinase (CK) levels were recorded in the wet than in the dry season. Body condition scores were positively correlated (P < 0.05) to albumin, body weight and packed cell volume. However, body condition scores were negatively correlated to TP, glucose, alanine transaminase (ALT) and AST. Strongyle egg loads were positively correlated to FAMACHA scores, packed cell volume, body weight and body condition score. The observed high globulin levels suggested a chronic health challenge. Thus, it is fundamental to devise affordable interventions for the control of gastrointestinal parasites in communal goats leading to improved goat productivity and hence rural livelihood
- Full Text:
- Date Issued: 2008
Community development education: the integration of individual and collective consciousness for community well-being within a social development paradigm in South Africa
- Authors: Maistry, Savathrie
- Date: 2008
- Subjects: Community development -- South Africa , Social planning -- South Africa , Public welfare -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD (Development Studies)
- Identifier: vital:11754 , http://hdl.handle.net/10353/182 , Community development -- South Africa , Social planning -- South Africa , Public welfare -- South Africa
- Description: This study focused on community development education and practice for the democratic and transformative South context. Social and community development are relatively new approaches to social welfare and community development is emerging as a discipline and profession to contribute to the goal of developmental social welfare. Community work, albeit to a minimal extent and not community development was viewed as a method of social work during apartheid. With community development being prioritized as an intervention strategy for poverty reduction by the national government, the gap in community development education needs to be addressed. The goal of the study was to develop an undergraduate curriculum framework for community development education that would produce a new generation of ethical community development professionals to contribute to the goal of developmental social welfare. The study focused on the integrated approach to community development education and practice; with education of the student as a whole human being as critical for community development in a transformative South Africa. To achieve its goal, the study identified three themes that were critical to the research: a conceptual framework, clarifying key terms; a curriculum framework for community development education; and qualities and ethics for a new generation of development practitioners. The qualitative study was based in the Eastern Cape Province, South Africa and because of the lack of a referential framework for community development education, the researcher looked towards learning from India which has over fifty years of experience in social and community development education and practice. The state of Kerala established a social development approach in 1957 and was chosen as a learning experience. The state’s Kudumbashree Mission which aims at poverty alleviation through the empowerment of women was chosen as a case study. The integral and values based education system of Sri Sathya Sai University in the state of Andhra Pradesh and the community development education and practice of Loyola College in the state of Kerala were examined. The research design was exploratory, conceptual, descriptive and developmental in nature. The qualitative methodology best suited the nature of this study and a combination of methods to obtain data from a variety of sources across national boundaries was employed. The participants interviewed in both countries were purposively selected for their involvement in social and community development directly as target groups, practitioners, managers and policy makers. The research produced a philosophical and theoretical framework that is unitary and integral and aligned with the current social development policy to guide community development education and practice. The unitary and integral framework encompasses the individual within the family and community context and locates the various dimensions of development such as the social, physical, cultural, psychological, spiritual, political and economical dimensions within a unitary platform. The framework is dominated by the holistic and humanistic philosophies without negating the rational and pragmatic perspectives. In conclusion, the study conceptualizes community development as a natural process; the integration of individual and collective consciousness and an intervention. The outcome of the study is a recommended curriculum framework for community development education that would be of relevance to the Eastern Cape Province in particular and for the South African context generally.
- Full Text:
- Date Issued: 2008
- Authors: Maistry, Savathrie
- Date: 2008
- Subjects: Community development -- South Africa , Social planning -- South Africa , Public welfare -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD (Development Studies)
- Identifier: vital:11754 , http://hdl.handle.net/10353/182 , Community development -- South Africa , Social planning -- South Africa , Public welfare -- South Africa
- Description: This study focused on community development education and practice for the democratic and transformative South context. Social and community development are relatively new approaches to social welfare and community development is emerging as a discipline and profession to contribute to the goal of developmental social welfare. Community work, albeit to a minimal extent and not community development was viewed as a method of social work during apartheid. With community development being prioritized as an intervention strategy for poverty reduction by the national government, the gap in community development education needs to be addressed. The goal of the study was to develop an undergraduate curriculum framework for community development education that would produce a new generation of ethical community development professionals to contribute to the goal of developmental social welfare. The study focused on the integrated approach to community development education and practice; with education of the student as a whole human being as critical for community development in a transformative South Africa. To achieve its goal, the study identified three themes that were critical to the research: a conceptual framework, clarifying key terms; a curriculum framework for community development education; and qualities and ethics for a new generation of development practitioners. The qualitative study was based in the Eastern Cape Province, South Africa and because of the lack of a referential framework for community development education, the researcher looked towards learning from India which has over fifty years of experience in social and community development education and practice. The state of Kerala established a social development approach in 1957 and was chosen as a learning experience. The state’s Kudumbashree Mission which aims at poverty alleviation through the empowerment of women was chosen as a case study. The integral and values based education system of Sri Sathya Sai University in the state of Andhra Pradesh and the community development education and practice of Loyola College in the state of Kerala were examined. The research design was exploratory, conceptual, descriptive and developmental in nature. The qualitative methodology best suited the nature of this study and a combination of methods to obtain data from a variety of sources across national boundaries was employed. The participants interviewed in both countries were purposively selected for their involvement in social and community development directly as target groups, practitioners, managers and policy makers. The research produced a philosophical and theoretical framework that is unitary and integral and aligned with the current social development policy to guide community development education and practice. The unitary and integral framework encompasses the individual within the family and community context and locates the various dimensions of development such as the social, physical, cultural, psychological, spiritual, political and economical dimensions within a unitary platform. The framework is dominated by the holistic and humanistic philosophies without negating the rational and pragmatic perspectives. In conclusion, the study conceptualizes community development as a natural process; the integration of individual and collective consciousness and an intervention. The outcome of the study is a recommended curriculum framework for community development education that would be of relevance to the Eastern Cape Province in particular and for the South African context generally.
- Full Text:
- Date Issued: 2008
Decentralisation and development: the contradictions of local government in Uganda with specific reference to Masindi and Sembabule districts
- Authors: Galiwango, Wasswa Hassan
- Date: 2008
- Subjects: Decentralization in government -- Uganda , Local government -- Uganda , Uganda -- Politics and government
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8238 , http://hdl.handle.net/10948/780 , Decentralization in government -- Uganda , Local government -- Uganda , Uganda -- Politics and government
- Description: Decentralisation is the process through which Central Government transfers authority and functions to sub-national units of the Government and it traces its origin in Uganda from the “ bush” period (1981 – 1986) when Resistance Committees were established by the NRM/A in the Luwero triangle. The Mamdani Commission Report of 1987 on the Local Government system in Uganda recommended devolution of powers. Subsequently, decentralisation was launched in 1992, constitutionalised by the 1995 Constitution, and operationalised by the Local Governments Act (LGA) in 1997. Among the services devolved were education and health, which this study used as case studies to illustrate whether decentralisation has enhanced development in Uganda during the period 1993 – 2006. The study used both primary and secondary data in analysing the linkage between decentralisation and development in the two selected districts in Uganda, namely Masindi and Sembabule. Primary data was collected through interviews, questionnaires and focus group discussions while secondary data was gathered through a literature survey of relevant textbooks, newspapers, reports, legislation and journals. The findings of the study established that if decentralisation is properly planned and implemented it can make a meaningful contribution to enhancing development. However, since decentralisation is a process and not a once-off project, it evolves from one stage to another and, as it does so, it also unfolds new challenges and contradictions that need to be effectively addressed. These challenges include aspects relating to the legal framework, as well as political, fiscal and administrative decentralisation. The study recommended mitigation measures to enhance the efficiency, effectiveness, accountability, transparency, and subsequently the quality of services delivered (development) under decentralised local governance in Uganda.
- Full Text:
- Date Issued: 2008
- Authors: Galiwango, Wasswa Hassan
- Date: 2008
- Subjects: Decentralization in government -- Uganda , Local government -- Uganda , Uganda -- Politics and government
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8238 , http://hdl.handle.net/10948/780 , Decentralization in government -- Uganda , Local government -- Uganda , Uganda -- Politics and government
- Description: Decentralisation is the process through which Central Government transfers authority and functions to sub-national units of the Government and it traces its origin in Uganda from the “ bush” period (1981 – 1986) when Resistance Committees were established by the NRM/A in the Luwero triangle. The Mamdani Commission Report of 1987 on the Local Government system in Uganda recommended devolution of powers. Subsequently, decentralisation was launched in 1992, constitutionalised by the 1995 Constitution, and operationalised by the Local Governments Act (LGA) in 1997. Among the services devolved were education and health, which this study used as case studies to illustrate whether decentralisation has enhanced development in Uganda during the period 1993 – 2006. The study used both primary and secondary data in analysing the linkage between decentralisation and development in the two selected districts in Uganda, namely Masindi and Sembabule. Primary data was collected through interviews, questionnaires and focus group discussions while secondary data was gathered through a literature survey of relevant textbooks, newspapers, reports, legislation and journals. The findings of the study established that if decentralisation is properly planned and implemented it can make a meaningful contribution to enhancing development. However, since decentralisation is a process and not a once-off project, it evolves from one stage to another and, as it does so, it also unfolds new challenges and contradictions that need to be effectively addressed. These challenges include aspects relating to the legal framework, as well as political, fiscal and administrative decentralisation. The study recommended mitigation measures to enhance the efficiency, effectiveness, accountability, transparency, and subsequently the quality of services delivered (development) under decentralised local governance in Uganda.
- Full Text:
- Date Issued: 2008
Development of a novel in situ CPRG-based biosensor and bioprobe for monitoring coliform β-D-Galactosidase in water polluted by faecal matter
- Authors: Wutor, Victor Collins
- Date: 2008
- Subjects: Biosensors Molecular probes Enterobacteriaceae Feces -- Microbiology Water -- Pollution -- Environmental aspects Environmental monitoring Chromogenic compounds
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3944 , http://hdl.handle.net/10962/d1004003
- Description: The ultimate objective of this work was to develop a real-time method for detecting and monitoring β-D-galactosidase as a suitable indicator of the potential presence of total coliform bacteria in water environments. Preliminary comparison of the chromogenic substrate, chlorophenol red β-D-galactopyranoside and the fluorogenic substrate, MuGAL, revealed unreliable results with the fluorogenic technique due to interference from compounds commonly found in environmental water samples. Thus, the chromogenic assay was further explored. Hydrolysis of the chromogenic substrate chlorophenol red β-D-galactopyranoside by β-D-galactosidase to yield chlorophenol red was the basis of this assay. Fundamental studies with chlorophenol red β-Dgalactopyranoside showed that β-D-galactosidase occurs extracellularly and in low concentrations in the polluted water environment. A direct correlation between enzyme activity and an increase in environmental water sample volume, as well as enzyme activity with total coliform colony forming unit counts were observed. Spectrophotometric detection was achieved within a maximum period of 24 h with a limit of detection level of 1 colony forming unit 100 ml[superscript -1]. This enzyme also exhibited physical and kinetic properties different from those of the pure commercially available β-D-galactosidase. Cell permeabilisation was not required for releasing enzymes into the extracellular environment. PEG 20 000 offered the best option for concentrating β-D-galactosidase. The source of β-D-galactosidase in the polluted environmental water samples was confirmed as Escherichia coli through SDS-PAGE, tryptic mapping and MALDI-TOF, thus justifying the further use of this method for detecting and/or monitoring total coliforms. Several compounds and metal ions commonly found in environmental water samples (as well as those used in water treatment processes) did have an effect on β-D-galactosidase. All the divalent cations except Mg [superscript 2+], at the concentrations studied, inhibited the relative activity of β-D-galactosidase in both commercial β-D-galactosidase and environmental samples. Immobilisation of chlorophenol red β-D-galactopyranoside onto a solid support material for the development of a strip bioprobe was unsuccessful, even though the nylon support material yielded some positive results. A monthly (seasonal) variation in β-Dgalactosidase activity from the environmental water samples was observed, with the highest activity coinciding with the highest monthly temperatures. Electro-oxidative detection and/or monitoring of chlorophenol red was possible. Chlorophenol red detection was linear over a wide range of concentrations (0.001-0.01 μg ml[superscript -1]). Interference by chlorophenol red β-D-galactopyranoside in the reduction window affected analysis. A range of phthalocyanine metal complexes were studied in an attempt to reduce fouling and/or increase the sensitivity of the biosensor. The selected phthalocyanine metal complexes were generally sensitive to changes in pH with a reduction in sensitivity from acidic pH to alkaline pH. The tetrasulphonated phthalocyanine metal complex of copper was, however, more stable with a minimum change of sensitivity. The phthalocyanine metal complexes were generally stable to changes in temperature. While only two consecutive scans were possible with the unmodified glassy carbon electrode, 77 consecutive scans were performed successfully with the CuPc-modified glassy carbon electrode. Among the phthalocyanine metal complexes studied, the CuPc-modified glassy carbon electrode therefore provided excellent results for the development of a biosensor. The CuPc modified-glassy carbon electrode detected 1 colony forming unit 100 ml[superscript -1] in 15 minutes, while the plain unmodified glassy carbon electrode required 6 hours to detect the equivalent number of colony forming units. CoPc, ZnPc and CuTSPc required 2, 2.25 and 1.75 h, respectively, to detect the same numbers of colony forming units. The CuPcmodified glassy carbon electrode detected 40 colony forming units 100 ml[superscript -1] instantly. In general, a direct correlation between colony forming units and current generated in the sensor was observed (R2=0.92). A higher correlation coefficient of 0.99 for 0-30 coliform colony forming units 100 ml[superscript -1] was determined. Current was detected in some water samples which did not show any colony forming units on the media, probably due to the phenomenon of viable but non-culturable bacteria, which is the major disadvantage encountered in the use of media for detecting indicator microorganisms. This novel biosensor therefore presents a very robust and sensitive technique for the detection and/or monitoring of coliform bacterial activity in water.
- Full Text:
- Date Issued: 2008
- Authors: Wutor, Victor Collins
- Date: 2008
- Subjects: Biosensors Molecular probes Enterobacteriaceae Feces -- Microbiology Water -- Pollution -- Environmental aspects Environmental monitoring Chromogenic compounds
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3944 , http://hdl.handle.net/10962/d1004003
- Description: The ultimate objective of this work was to develop a real-time method for detecting and monitoring β-D-galactosidase as a suitable indicator of the potential presence of total coliform bacteria in water environments. Preliminary comparison of the chromogenic substrate, chlorophenol red β-D-galactopyranoside and the fluorogenic substrate, MuGAL, revealed unreliable results with the fluorogenic technique due to interference from compounds commonly found in environmental water samples. Thus, the chromogenic assay was further explored. Hydrolysis of the chromogenic substrate chlorophenol red β-D-galactopyranoside by β-D-galactosidase to yield chlorophenol red was the basis of this assay. Fundamental studies with chlorophenol red β-Dgalactopyranoside showed that β-D-galactosidase occurs extracellularly and in low concentrations in the polluted water environment. A direct correlation between enzyme activity and an increase in environmental water sample volume, as well as enzyme activity with total coliform colony forming unit counts were observed. Spectrophotometric detection was achieved within a maximum period of 24 h with a limit of detection level of 1 colony forming unit 100 ml[superscript -1]. This enzyme also exhibited physical and kinetic properties different from those of the pure commercially available β-D-galactosidase. Cell permeabilisation was not required for releasing enzymes into the extracellular environment. PEG 20 000 offered the best option for concentrating β-D-galactosidase. The source of β-D-galactosidase in the polluted environmental water samples was confirmed as Escherichia coli through SDS-PAGE, tryptic mapping and MALDI-TOF, thus justifying the further use of this method for detecting and/or monitoring total coliforms. Several compounds and metal ions commonly found in environmental water samples (as well as those used in water treatment processes) did have an effect on β-D-galactosidase. All the divalent cations except Mg [superscript 2+], at the concentrations studied, inhibited the relative activity of β-D-galactosidase in both commercial β-D-galactosidase and environmental samples. Immobilisation of chlorophenol red β-D-galactopyranoside onto a solid support material for the development of a strip bioprobe was unsuccessful, even though the nylon support material yielded some positive results. A monthly (seasonal) variation in β-Dgalactosidase activity from the environmental water samples was observed, with the highest activity coinciding with the highest monthly temperatures. Electro-oxidative detection and/or monitoring of chlorophenol red was possible. Chlorophenol red detection was linear over a wide range of concentrations (0.001-0.01 μg ml[superscript -1]). Interference by chlorophenol red β-D-galactopyranoside in the reduction window affected analysis. A range of phthalocyanine metal complexes were studied in an attempt to reduce fouling and/or increase the sensitivity of the biosensor. The selected phthalocyanine metal complexes were generally sensitive to changes in pH with a reduction in sensitivity from acidic pH to alkaline pH. The tetrasulphonated phthalocyanine metal complex of copper was, however, more stable with a minimum change of sensitivity. The phthalocyanine metal complexes were generally stable to changes in temperature. While only two consecutive scans were possible with the unmodified glassy carbon electrode, 77 consecutive scans were performed successfully with the CuPc-modified glassy carbon electrode. Among the phthalocyanine metal complexes studied, the CuPc-modified glassy carbon electrode therefore provided excellent results for the development of a biosensor. The CuPc modified-glassy carbon electrode detected 1 colony forming unit 100 ml[superscript -1] in 15 minutes, while the plain unmodified glassy carbon electrode required 6 hours to detect the equivalent number of colony forming units. CoPc, ZnPc and CuTSPc required 2, 2.25 and 1.75 h, respectively, to detect the same numbers of colony forming units. The CuPcmodified glassy carbon electrode detected 40 colony forming units 100 ml[superscript -1] instantly. In general, a direct correlation between colony forming units and current generated in the sensor was observed (R2=0.92). A higher correlation coefficient of 0.99 for 0-30 coliform colony forming units 100 ml[superscript -1] was determined. Current was detected in some water samples which did not show any colony forming units on the media, probably due to the phenomenon of viable but non-culturable bacteria, which is the major disadvantage encountered in the use of media for detecting indicator microorganisms. This novel biosensor therefore presents a very robust and sensitive technique for the detection and/or monitoring of coliform bacterial activity in water.
- Full Text:
- Date Issued: 2008
Development of a reciprocating aerofoil wind energy harvester
- Authors: Phillips, Russell Leslie
- Date: 2008
- Subjects: Windmills , Wind power , Wind turbines
- Language: English
- Type: Thesis , Doctoral , DTech
- Identifier: vital:9622 , http://hdl.handle.net/10948/899 , Windmills , Wind power , Wind turbines
- Description: Cross flow wind turbines are not unique. The performance of Savonius and Darrieus turbines is well documented. Both share the advantage of being able to accept fluid flow from any direction. The Savonius is drag based and hence has poor power output while the Darrieus is lift based. Due to the fact that the Darrieus has fixed blades the fluid flow through the rotor does not result in optimal lift being generated at all points in the rotation circle. A drawback of the Darrieus system is that it has to operate at a high tip-to wind-speed ratio to obtain reasonable performance with the fixed blades. Deviation from a small optimal range of tip speed ratios results in poor performance. The Darrieus also has poor starting torque. The research conducted in this project focused on overcoming the shortcomings of other turbines and developing an effective cross flow turbine capable of good performance. A number of different concepts were experimented with, however all were based on a symmetrical aerofoil presented to the actual relative airflow at an angle that would produce the highest lift force at all times. The lift force was then utilized to generate movement and to do work on an electrical generator. All concepts contemplated were researched to ascertain their appropriateness for the intended application. During development of the final experimental platform and after lodging of a provisional patent (RSA 2007/00927) it was ascertained that the design shared some similarities with an American patent 5503525 dated 28/4/1994. This patent employed complex electronic sensing and control equipment for control of blade angle. This was thought to be overly complex and costly, particularly for small scale wind energy generation applications and a simpler mechanical solution was sought in the design of the final experimental platform used in this project. The design of the mechanical control system was refined in an attempt to make it simpler, more durable and employ the least number of moving parts. Literature studies and patent searches conducted, suggested that the mechanical control system as developed for the final experimental platform was unique. The enormous variation in the power available from the wind at the different wind speeds likely to be encountered by the device necessitated some means of control. In high wind conditions control of the amount of wind power into the device was deemed to be the preferable means of control. A number of different concepts to achieve this were devised and tested. The final concept employed limited the tail angle deflection and hence the lift produced by the aerofoils. This resulted in a seamless “throttle” control allowing the device to be used in any wind strength by adjusting the control to a position that resulted in the device receiving a suitable amount of power from the wind. The outcome of performance tests conducted indicated that the device has the potential to be developed into a viable wind turbine for both small and large scale applications. The ability to control the power input from the wind to the machine from zero to a maximum is considered to be one of the most beneficial outcomes of this project and together with the quiet operation and low speed, are considered the main advantages of the device over existing wind turbine designs. The possibilities of using the device to compress air for energy storage are exciting avenues that warrant further research.
- Full Text:
- Date Issued: 2008
- Authors: Phillips, Russell Leslie
- Date: 2008
- Subjects: Windmills , Wind power , Wind turbines
- Language: English
- Type: Thesis , Doctoral , DTech
- Identifier: vital:9622 , http://hdl.handle.net/10948/899 , Windmills , Wind power , Wind turbines
- Description: Cross flow wind turbines are not unique. The performance of Savonius and Darrieus turbines is well documented. Both share the advantage of being able to accept fluid flow from any direction. The Savonius is drag based and hence has poor power output while the Darrieus is lift based. Due to the fact that the Darrieus has fixed blades the fluid flow through the rotor does not result in optimal lift being generated at all points in the rotation circle. A drawback of the Darrieus system is that it has to operate at a high tip-to wind-speed ratio to obtain reasonable performance with the fixed blades. Deviation from a small optimal range of tip speed ratios results in poor performance. The Darrieus also has poor starting torque. The research conducted in this project focused on overcoming the shortcomings of other turbines and developing an effective cross flow turbine capable of good performance. A number of different concepts were experimented with, however all were based on a symmetrical aerofoil presented to the actual relative airflow at an angle that would produce the highest lift force at all times. The lift force was then utilized to generate movement and to do work on an electrical generator. All concepts contemplated were researched to ascertain their appropriateness for the intended application. During development of the final experimental platform and after lodging of a provisional patent (RSA 2007/00927) it was ascertained that the design shared some similarities with an American patent 5503525 dated 28/4/1994. This patent employed complex electronic sensing and control equipment for control of blade angle. This was thought to be overly complex and costly, particularly for small scale wind energy generation applications and a simpler mechanical solution was sought in the design of the final experimental platform used in this project. The design of the mechanical control system was refined in an attempt to make it simpler, more durable and employ the least number of moving parts. Literature studies and patent searches conducted, suggested that the mechanical control system as developed for the final experimental platform was unique. The enormous variation in the power available from the wind at the different wind speeds likely to be encountered by the device necessitated some means of control. In high wind conditions control of the amount of wind power into the device was deemed to be the preferable means of control. A number of different concepts to achieve this were devised and tested. The final concept employed limited the tail angle deflection and hence the lift produced by the aerofoils. This resulted in a seamless “throttle” control allowing the device to be used in any wind strength by adjusting the control to a position that resulted in the device receiving a suitable amount of power from the wind. The outcome of performance tests conducted indicated that the device has the potential to be developed into a viable wind turbine for both small and large scale applications. The ability to control the power input from the wind to the machine from zero to a maximum is considered to be one of the most beneficial outcomes of this project and together with the quiet operation and low speed, are considered the main advantages of the device over existing wind turbine designs. The possibilities of using the device to compress air for energy storage are exciting avenues that warrant further research.
- Full Text:
- Date Issued: 2008
Effects of coastal topography on physiology, behaviour and genetics of indigenous (Perna perna) and invasive (Mytilus galloprovincialis) mussels
- Authors: Nicastro, Katy R
- Date: 2008
- Subjects: Mussels -- Ecology -- South Africa , Perna -- Physiology -- South Africa , Perna -- Behavior -- South Africa , Mussels -- Behavior -- Environmental factors -- South Africa , Mussels -- Habitat -- South Africa , Mytilus galloprovincialis , Mytilus galloprovincialis -- Physiology -- South Africa , Mytilus galloprovincialis -- Behavior -- South Africa , Mytilus galloprovincialis -- Genetics -- South Africa , Coastal ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5833 , http://hdl.handle.net/10962/d1008262
- Description: Organisms inhabit environments that have many dimensions, each of which can vary temporally and spatially. The spatial-temporal variations of environmental stressors and disturbances may have major but different effects on indigenous and invasive species, favouring either of them at different times and places. The invasive mussel Mytilus galloprovincialis invaded the South African coast 30 years ago and, on the south coast of South Africa, it now competes and co-exists with the indigenous Perna perna in the lower eulittoral zone (referred to here as the mussel zone) The invasive and indigenous species dominate the upper and the lower mussel zones respectively, while the two co-exist in the mid-zone. My results show that intertidal mussels experience, and respond to, spatial and temporal fluctuations of several biotic and abiotic stressors. The invasive and the indigenous species adopt different strategies when reacting to environmental factors and their physiological and behavioural responses vary in time and in different habitats as different pressures become of overriding importance. Attachment strength of both species decreased in summer and increased in winter, and was higher on the open coast than in bays for both species, showing a strong positive correlation with wave force in time and space. P. perna had significantly higher attachment strength than M. galloprovincialis but, contrary to previous studies, the difference in gonad index between the two species varied according to the habitat. In bay habitats, M. galloprovincialis had a higher maximum reproductive effort than P. perna, however, on the open coast, there was no significant difference between the two species, suggesting that for the invasive species wave action is a limiting factor not only in terms of the attachment strength but also of energy availability for reproductive tissue development. Major spawning events occurred during periods of low wave action while minor spawning coincided with periods of intense hydrodynamic stress. On the open coast, gonad index was negatively correlated with attachment strength for both species while, in bays, there was no correlation between these two factors for either. The two species also showed different behaviour. In the field, M. galloprovincialis moved significantly more than P. perna over a period of six months. The higher mobility of the invasive species was also confirmed in the laboratory where, in general, M. galloprovincialis formed clumps more readily than P. perna. Taken collectively, these results suggest that channelling more energy into attachment strength limits reproductive tissue development and that, while the indigenous species invests more in byssal production, the invasive species adopts a more dynamic strategy looking for aggregation or a safer arrangement. Higher endolithic infestation and a greater expression of heat shock proteins (Hsps) in mussel populations on the open coast than in bays indicate that this habitat is a more stressful environment not only in terms of wave action. Endolith damaged mussels had significantly lower attachment strengths and condition indices than clean mussels, probably due to the need to channel energy into shell repair. The constant shell repair and expression of Hsps typical of open coast populations are energetically demanding processes. These observations suggest that on the open coast, mussels are subjected to more severe energetic constraints than in bay habitats. Wave and sand stress fluctuated seasonally with the former having a greater effect on mussel mortality on the open coast and the latter a higher impact on bay populations. Overall, mussel mortality rates were higher on the open coast than in bays. My results show that populations on the open coast had fewer private haplotypes and less genetic endemism than those inside bays. Gene flow analysis showed the relatively stable bay habitats act as source populations with greater genetic migration rates out of bays than into them. These differences in genetic structure on scales of las of kilometers show that coastal configuration strongly affects selection, larval dispersal and haplotype diversity. Environmental gradients that are key factors in species distribution over large geographical scales can also be responsible for micro-scale distributions. My results show that M. galloprovincialis colonizes the upper mussel zone where temperature is high, but is less tolerant to this stressor and has to maintain a high expression of Hsps. This suggests that temperature is probably a limiting factor in its invasion towards the sub-tropical east coast. There are inter- and intra-specific differences in responses to the environment which highlight the efforts of M. galloprovincialis and P. perna to optimize resource utilization for survival and reproduction. Determining these differences is crucial to understanding patterns of co-existence between competing indigenous and invasive species.
- Full Text:
- Date Issued: 2008
- Authors: Nicastro, Katy R
- Date: 2008
- Subjects: Mussels -- Ecology -- South Africa , Perna -- Physiology -- South Africa , Perna -- Behavior -- South Africa , Mussels -- Behavior -- Environmental factors -- South Africa , Mussels -- Habitat -- South Africa , Mytilus galloprovincialis , Mytilus galloprovincialis -- Physiology -- South Africa , Mytilus galloprovincialis -- Behavior -- South Africa , Mytilus galloprovincialis -- Genetics -- South Africa , Coastal ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5833 , http://hdl.handle.net/10962/d1008262
- Description: Organisms inhabit environments that have many dimensions, each of which can vary temporally and spatially. The spatial-temporal variations of environmental stressors and disturbances may have major but different effects on indigenous and invasive species, favouring either of them at different times and places. The invasive mussel Mytilus galloprovincialis invaded the South African coast 30 years ago and, on the south coast of South Africa, it now competes and co-exists with the indigenous Perna perna in the lower eulittoral zone (referred to here as the mussel zone) The invasive and indigenous species dominate the upper and the lower mussel zones respectively, while the two co-exist in the mid-zone. My results show that intertidal mussels experience, and respond to, spatial and temporal fluctuations of several biotic and abiotic stressors. The invasive and the indigenous species adopt different strategies when reacting to environmental factors and their physiological and behavioural responses vary in time and in different habitats as different pressures become of overriding importance. Attachment strength of both species decreased in summer and increased in winter, and was higher on the open coast than in bays for both species, showing a strong positive correlation with wave force in time and space. P. perna had significantly higher attachment strength than M. galloprovincialis but, contrary to previous studies, the difference in gonad index between the two species varied according to the habitat. In bay habitats, M. galloprovincialis had a higher maximum reproductive effort than P. perna, however, on the open coast, there was no significant difference between the two species, suggesting that for the invasive species wave action is a limiting factor not only in terms of the attachment strength but also of energy availability for reproductive tissue development. Major spawning events occurred during periods of low wave action while minor spawning coincided with periods of intense hydrodynamic stress. On the open coast, gonad index was negatively correlated with attachment strength for both species while, in bays, there was no correlation between these two factors for either. The two species also showed different behaviour. In the field, M. galloprovincialis moved significantly more than P. perna over a period of six months. The higher mobility of the invasive species was also confirmed in the laboratory where, in general, M. galloprovincialis formed clumps more readily than P. perna. Taken collectively, these results suggest that channelling more energy into attachment strength limits reproductive tissue development and that, while the indigenous species invests more in byssal production, the invasive species adopts a more dynamic strategy looking for aggregation or a safer arrangement. Higher endolithic infestation and a greater expression of heat shock proteins (Hsps) in mussel populations on the open coast than in bays indicate that this habitat is a more stressful environment not only in terms of wave action. Endolith damaged mussels had significantly lower attachment strengths and condition indices than clean mussels, probably due to the need to channel energy into shell repair. The constant shell repair and expression of Hsps typical of open coast populations are energetically demanding processes. These observations suggest that on the open coast, mussels are subjected to more severe energetic constraints than in bay habitats. Wave and sand stress fluctuated seasonally with the former having a greater effect on mussel mortality on the open coast and the latter a higher impact on bay populations. Overall, mussel mortality rates were higher on the open coast than in bays. My results show that populations on the open coast had fewer private haplotypes and less genetic endemism than those inside bays. Gene flow analysis showed the relatively stable bay habitats act as source populations with greater genetic migration rates out of bays than into them. These differences in genetic structure on scales of las of kilometers show that coastal configuration strongly affects selection, larval dispersal and haplotype diversity. Environmental gradients that are key factors in species distribution over large geographical scales can also be responsible for micro-scale distributions. My results show that M. galloprovincialis colonizes the upper mussel zone where temperature is high, but is less tolerant to this stressor and has to maintain a high expression of Hsps. This suggests that temperature is probably a limiting factor in its invasion towards the sub-tropical east coast. There are inter- and intra-specific differences in responses to the environment which highlight the efforts of M. galloprovincialis and P. perna to optimize resource utilization for survival and reproduction. Determining these differences is crucial to understanding patterns of co-existence between competing indigenous and invasive species.
- Full Text:
- Date Issued: 2008
Effects of temperature on the development, behaviour and geography of blowflies in a forensic context
- Authors: Richards, Cameron Spencer
- Date: 2008
- Subjects: Blowflies Blowflies -- South Africa Diptera -- South Africa Insects -- Development Insects -- Behavior Forensic entomology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5683 , http://hdl.handle.net/10962/d1005369
- Description: The development of immature insects is commonly employed in forensic investigations to estimate time of death, or postmortem interval (PMI), of a corpse on which they are feeding. The bulk of this thesis focuses on factors influencing the accuracy of developmental data, and exploring how and why developmental data differ between studies involving the same species, and between different species. Because carrion feeding insects are ectotherms, temperature may be expected to significantly influence their behaviour, development and distribution, and the remainder of the thesis therefore focuses on the thermal biology and geographical distribution of seven forensically important blowflies. The species include Chrysomya albiceps, C. putoria, C. chloropyga, C. megacephala, C. marginalis, C. inclinata and Calliphora croceipalpis. A robust experimental design for estimating developmental models is outlined and tested. It is recommended that forensic entomologists should involve at least six constant temperatures, starting at about 7°C above the relevant developmental zero (D0) and going to about 10°C above the upper critical temperature, and a temporal sampling interval with a relative precision of about 10%. Using this design, focused experiments consistently provided the most reliable developmental data, while data pooled from different studies yielded inconsistent results. Similarly, developmental data from closely related species differed significantly, and surprisingly so did developmental data from different populations of the same species. Possible explanations for the latter lay in the different methods of data collection but only temporal sampling resolution had a direct influence on the accuracy of developmental data. Consequently, disparities in such data were primarily ascribed to genetic differences and phenotypic plasticity. Comparisons between numerous thermal thresholds of larvae, pupae and adults support this conclusion and suggest a phylogenetic component to the thermal biology of blowflies. Further comparisons were made between these temperature thresholds and the distributions of blowfly species present on two rhinoceros carcasses. These comparisons suggest that blowfly larvae with high upper lethal temperature thresholds dominate in interspecific competition in favorable thermal environments by raising maggot mass temperature above the thresholds of other carrion-feeding blowflies, through maggot-generated heat. Bioclimatic modeling using maximum entropy analysis provided a successful means of predicting whether a species is likely to occur in an area, and whether it would therefore be expected in a local carcass community. It also showed that temperature was less important than moisture in shaping the geographical distribution of African carrion blowflies. Based on these results, several recommendations are made for the practice of forensic entomology.
- Full Text:
- Date Issued: 2008
- Authors: Richards, Cameron Spencer
- Date: 2008
- Subjects: Blowflies Blowflies -- South Africa Diptera -- South Africa Insects -- Development Insects -- Behavior Forensic entomology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5683 , http://hdl.handle.net/10962/d1005369
- Description: The development of immature insects is commonly employed in forensic investigations to estimate time of death, or postmortem interval (PMI), of a corpse on which they are feeding. The bulk of this thesis focuses on factors influencing the accuracy of developmental data, and exploring how and why developmental data differ between studies involving the same species, and between different species. Because carrion feeding insects are ectotherms, temperature may be expected to significantly influence their behaviour, development and distribution, and the remainder of the thesis therefore focuses on the thermal biology and geographical distribution of seven forensically important blowflies. The species include Chrysomya albiceps, C. putoria, C. chloropyga, C. megacephala, C. marginalis, C. inclinata and Calliphora croceipalpis. A robust experimental design for estimating developmental models is outlined and tested. It is recommended that forensic entomologists should involve at least six constant temperatures, starting at about 7°C above the relevant developmental zero (D0) and going to about 10°C above the upper critical temperature, and a temporal sampling interval with a relative precision of about 10%. Using this design, focused experiments consistently provided the most reliable developmental data, while data pooled from different studies yielded inconsistent results. Similarly, developmental data from closely related species differed significantly, and surprisingly so did developmental data from different populations of the same species. Possible explanations for the latter lay in the different methods of data collection but only temporal sampling resolution had a direct influence on the accuracy of developmental data. Consequently, disparities in such data were primarily ascribed to genetic differences and phenotypic plasticity. Comparisons between numerous thermal thresholds of larvae, pupae and adults support this conclusion and suggest a phylogenetic component to the thermal biology of blowflies. Further comparisons were made between these temperature thresholds and the distributions of blowfly species present on two rhinoceros carcasses. These comparisons suggest that blowfly larvae with high upper lethal temperature thresholds dominate in interspecific competition in favorable thermal environments by raising maggot mass temperature above the thresholds of other carrion-feeding blowflies, through maggot-generated heat. Bioclimatic modeling using maximum entropy analysis provided a successful means of predicting whether a species is likely to occur in an area, and whether it would therefore be expected in a local carcass community. It also showed that temperature was less important than moisture in shaping the geographical distribution of African carrion blowflies. Based on these results, several recommendations are made for the practice of forensic entomology.
- Full Text:
- Date Issued: 2008
Exploring the construct-related validity of the eye and hand coordination subscale of the Griffiths Mental Development Scales-Extended revised (GMDS-ER)
- Authors: Povey, Jenny-Louise
- Date: 2008
- Subjects: Griffiths Scales of Mental Development , Mental development -- Testing , Psychological tests for children , Children -- Intelligence testing -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9923 , http://hdl.handle.net/10948/661 , Griffiths Scales of Mental Development , Mental development -- Testing , Psychological tests for children , Children -- Intelligence testing -- South Africa
- Description: To constantly add to our understanding of child development, a valid, comprehensive, well-researched measure is needed. The recent revision and standardisation of the Griffiths Mental Development Scales - Extended Revised (GMDS-ER) has necessitated investigations into its psychometric properties. This measure is used to identify developmental delays in children and critical decisions may be made, hence it is imperative that this measure be deemed both reliable and valid. This study aimed to contribute to the broader restandardisation project and focussed on gathering qualitative and quantitative construct-related validity evidence for one of the six Subscales of the GMDS-ER, namely the Eye and Hand Coordination Subscale (Subscale D). An exploratory descriptive method using a triangulation approach was used to explore the construct-related validity evidence of the Eye and Hand Coordination Subscale. A non-random purposively selected sample of 6 experts, who had worked at least 10 years with children assessing and evaluating the domain of eye-hand coordination, participated in a facet analysis to identify the underlying dimensions tapped by the Eye and Hand Coordination Subscale. The sample for the quantitative aspects of this study, the empirical validation of the construct model, was collected as part of the broader restandardisation and represented a stratified sample of 1026 children between the ages 24 months and 96 months from across the United Kingdom and Eire. Three measures, namely a biographical questionnaire, the GMDS-ER and a construct evaluation form were used to gather the qualitative and quantitative data. The qualitative data was analysed by means of a facet analysis and literature control which included examining other tests or subtests that tapped eye-hand coordination skills. The quantitative data was analysed by means of exploratory common factor analysis using oblique (DQUART) rotation in order to verify the qualitatively identified construct model by specifying a one-factor solution for each underlying construct. While literature suggests that eye-hand coordination is multidimensional in nature the facet analysis revealed that the tasks tapped by Subscale D were not tapping complex skills aside from eye-hand coordination. The facet analysis thus revealed that all the items tap the eye-hand coordination of fine motor tasks and that these items could be grouped into two categories, using writing utensils and object manipulation. The results from the factor analysis confirmed that the items on Subscale D have one underlying construct and further exploration confirmed that these items could in fact be grouped into two categories. The coefficients of congruence for SES and gender were satisfactory and thus the construct model was validated across these groups as well. The study has provided extensive qualitative and quantitative evidence for the construct-related validity of the Eye and Hand Coordination Subscale of the GMDS-ER, thus validating its use as a sound measure of eye-hand coordination development in children aged 2 to 8 years. The content coverage however, seemed to indicate that the tasks required do not adequately cover all the facets of eye-hand coordination and some of these item types are tapped by the other Subscales in the measure. Hence, should the Subscale be used as an independent assessment of eye-hand coordination, the eye-hand coordination development of the child would have a narrow focus. In addition, in reviewing the variety of tasks in the two sections for the older and younger child, the researcher noted that while section III tapped a variety of skills, section IV only focused on skills associated with drawing, copying and writing. A review of the age appropriateness of the items and the item analysis results from the revision seemed to indicate that some of the items assessing the older child may need to be revised. Further, the researcher suggested that the revision should consider adding additional dimensions to the tasks assessed. Overall, it is the researcher‟s opinion that this Subscale was not given adequate attention during the revision process and should future revisions occur, the recommendations from this study should be considered.
- Full Text:
- Date Issued: 2008
- Authors: Povey, Jenny-Louise
- Date: 2008
- Subjects: Griffiths Scales of Mental Development , Mental development -- Testing , Psychological tests for children , Children -- Intelligence testing -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9923 , http://hdl.handle.net/10948/661 , Griffiths Scales of Mental Development , Mental development -- Testing , Psychological tests for children , Children -- Intelligence testing -- South Africa
- Description: To constantly add to our understanding of child development, a valid, comprehensive, well-researched measure is needed. The recent revision and standardisation of the Griffiths Mental Development Scales - Extended Revised (GMDS-ER) has necessitated investigations into its psychometric properties. This measure is used to identify developmental delays in children and critical decisions may be made, hence it is imperative that this measure be deemed both reliable and valid. This study aimed to contribute to the broader restandardisation project and focussed on gathering qualitative and quantitative construct-related validity evidence for one of the six Subscales of the GMDS-ER, namely the Eye and Hand Coordination Subscale (Subscale D). An exploratory descriptive method using a triangulation approach was used to explore the construct-related validity evidence of the Eye and Hand Coordination Subscale. A non-random purposively selected sample of 6 experts, who had worked at least 10 years with children assessing and evaluating the domain of eye-hand coordination, participated in a facet analysis to identify the underlying dimensions tapped by the Eye and Hand Coordination Subscale. The sample for the quantitative aspects of this study, the empirical validation of the construct model, was collected as part of the broader restandardisation and represented a stratified sample of 1026 children between the ages 24 months and 96 months from across the United Kingdom and Eire. Three measures, namely a biographical questionnaire, the GMDS-ER and a construct evaluation form were used to gather the qualitative and quantitative data. The qualitative data was analysed by means of a facet analysis and literature control which included examining other tests or subtests that tapped eye-hand coordination skills. The quantitative data was analysed by means of exploratory common factor analysis using oblique (DQUART) rotation in order to verify the qualitatively identified construct model by specifying a one-factor solution for each underlying construct. While literature suggests that eye-hand coordination is multidimensional in nature the facet analysis revealed that the tasks tapped by Subscale D were not tapping complex skills aside from eye-hand coordination. The facet analysis thus revealed that all the items tap the eye-hand coordination of fine motor tasks and that these items could be grouped into two categories, using writing utensils and object manipulation. The results from the factor analysis confirmed that the items on Subscale D have one underlying construct and further exploration confirmed that these items could in fact be grouped into two categories. The coefficients of congruence for SES and gender were satisfactory and thus the construct model was validated across these groups as well. The study has provided extensive qualitative and quantitative evidence for the construct-related validity of the Eye and Hand Coordination Subscale of the GMDS-ER, thus validating its use as a sound measure of eye-hand coordination development in children aged 2 to 8 years. The content coverage however, seemed to indicate that the tasks required do not adequately cover all the facets of eye-hand coordination and some of these item types are tapped by the other Subscales in the measure. Hence, should the Subscale be used as an independent assessment of eye-hand coordination, the eye-hand coordination development of the child would have a narrow focus. In addition, in reviewing the variety of tasks in the two sections for the older and younger child, the researcher noted that while section III tapped a variety of skills, section IV only focused on skills associated with drawing, copying and writing. A review of the age appropriateness of the items and the item analysis results from the revision seemed to indicate that some of the items assessing the older child may need to be revised. Further, the researcher suggested that the revision should consider adding additional dimensions to the tasks assessed. Overall, it is the researcher‟s opinion that this Subscale was not given adequate attention during the revision process and should future revisions occur, the recommendations from this study should be considered.
- Full Text:
- Date Issued: 2008
Facilitating reflection in post-graduate writing practice
- Authors: Naidoo, Nadasen Arungasen
- Date: 2008
- Subjects: Academic writing , Report writing , Academic writing -- Study and teaching (Higher) -- South Africa , Written communication -- Study and teaching -- South Africa
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:9473 , http://hdl.handle.net/10948/688 , Academic writing , Report writing , Academic writing -- Study and teaching (Higher) -- South Africa , Written communication -- Study and teaching -- South Africa
- Description: University teaching staff are employed because of their knowledge in their particular disciplines. Many do not have a qualification to teach at a higher education institution upon commencement of their academic career. In that group there are few who have the research experience required to assist at postgraduate level. This should be developed as one of the three core activities of higher education, in which they have to be involved. This study is the result of a problem that I encountered as a higher education practitioner. In keeping with my being a practitioner researcher within an action research paradigm, this report is written mainly in the first person. The study reports on how my personal theories grew over a period resulting in the need to constantly improve my own practice. These personal theories culminated in the development of an instrument (ADaM), to assess writing. ADaM was used primarily to facilitate reflection in post-graduate writing practice. In this study, there were three sets of workshops comprising 13 practical sessions each, where lecturers engaged with the process of reading, writing, computer-mediation and, to a limited extent, with the concept of mentorship. The purpose was to answer the research question: Can a writing assessment instrument be used to sensitise staff teaching post-graduates to reflect on the complex nature of producing and assessing academic writing? At two points during the 13 practical sessions, data was gathered through semistructured interviews. The data has been analysed using a form of grounded theory referred to as remodeled grounded theory. Since the analysis traversed both the quantitative and qualitative paradigms of research, it was necessary also to place the study within the third paradigm, referred to as mixed methods research. The analysis has been presented via a series of relationships generated first by open coding, then axial coding and concludes with selective coding. In addition, the comments of an independent coder were used to validate the analysis. In accordance with classic grounded theory, it was only after the analysis of the data and the emergence of a substantive theory that I referred to existing theory in the penultimate chapter as validation of my findings. The findings from the study, together with existing literature, allowed me to conclude that “Creating an awareness of writing assessment sensitises academics to their roles as HE practitioners particularly in the areas of writing and mentorship in post-graduate supervision”.
- Full Text:
- Date Issued: 2008
- Authors: Naidoo, Nadasen Arungasen
- Date: 2008
- Subjects: Academic writing , Report writing , Academic writing -- Study and teaching (Higher) -- South Africa , Written communication -- Study and teaching -- South Africa
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:9473 , http://hdl.handle.net/10948/688 , Academic writing , Report writing , Academic writing -- Study and teaching (Higher) -- South Africa , Written communication -- Study and teaching -- South Africa
- Description: University teaching staff are employed because of their knowledge in their particular disciplines. Many do not have a qualification to teach at a higher education institution upon commencement of their academic career. In that group there are few who have the research experience required to assist at postgraduate level. This should be developed as one of the three core activities of higher education, in which they have to be involved. This study is the result of a problem that I encountered as a higher education practitioner. In keeping with my being a practitioner researcher within an action research paradigm, this report is written mainly in the first person. The study reports on how my personal theories grew over a period resulting in the need to constantly improve my own practice. These personal theories culminated in the development of an instrument (ADaM), to assess writing. ADaM was used primarily to facilitate reflection in post-graduate writing practice. In this study, there were three sets of workshops comprising 13 practical sessions each, where lecturers engaged with the process of reading, writing, computer-mediation and, to a limited extent, with the concept of mentorship. The purpose was to answer the research question: Can a writing assessment instrument be used to sensitise staff teaching post-graduates to reflect on the complex nature of producing and assessing academic writing? At two points during the 13 practical sessions, data was gathered through semistructured interviews. The data has been analysed using a form of grounded theory referred to as remodeled grounded theory. Since the analysis traversed both the quantitative and qualitative paradigms of research, it was necessary also to place the study within the third paradigm, referred to as mixed methods research. The analysis has been presented via a series of relationships generated first by open coding, then axial coding and concludes with selective coding. In addition, the comments of an independent coder were used to validate the analysis. In accordance with classic grounded theory, it was only after the analysis of the data and the emergence of a substantive theory that I referred to existing theory in the penultimate chapter as validation of my findings. The findings from the study, together with existing literature, allowed me to conclude that “Creating an awareness of writing assessment sensitises academics to their roles as HE practitioners particularly in the areas of writing and mentorship in post-graduate supervision”.
- Full Text:
- Date Issued: 2008
Floating sulphur biofilms structure, function and biotechnology
- Molwantwa, Jennifer Balatedi
- Authors: Molwantwa, Jennifer Balatedi
- Date: 2008
- Subjects: Biofilms Sulfur Acid mine drainage -- South Africa Mine water -- Purification -- Biological treatment Microbial ecology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3958 , http://hdl.handle.net/10962/d1004017
- Description: Mine wastewaters generated during active production operations, and decanting streams following mine closure have major environmental impacts, and volumes requiring treatment are expected to increase substantially as the South African mining industry matures. Biological treatment of mine waters has been the subject of increasing interest, where sulphate reducing bacteria are employed for the reduction of sulphate to sulphide, precipitation of metals and the production of alkalinity. However, the sulphide if not removed from the system can be oxidised back to sulphate. As a result there have been limitations especially in the provision of technological options that are sustainable over the long-term, where the total sulphur (in its different forms) can be removed from the system. These, however, are the subject of a number of constraints including, importantly, the process capability to remove reduced sulphur from the treated stream, in one of its oxidation states, and thus linearise the biological sulphur cycle. This remains a major bottleneck in the development of biological wastewater treatment technology. Floating sulphur biofilms are observed as surface layers in numerous aquatic sulphide-rich environments, and it has been suggested that they play a role in the biological cycling of sulphur. The use of sulphur biofilms for the removal of elemental sulphur was identified in this study as a possible means for addressing the technological bottleneck, especially in passive wastewater treatment systems. There is, however, little documented information in the literature on the structure of floating sulphur biofilms, the microbial species responsible for their occurrence or bio-process applications of the system. A linear flow channel reactor was developed to simulate natural conditions and enabled the study of floating sulphur biofilm under controlled laboratory conditions. It was observed that these biofilms developed through three distinct stages termed Thin, Sticky and Brittle films. A microprobe study showed the presence of a steep Redox gradient established across (260 to 380 μm) depth of the floating sulphur biofilm of ~ 0 to -200 mV (top to bottom), which correlated with pH and sulphide gradients across the system. Structural investigations embedded in an exopolymeric matrix containing clearly defined channels and pores. Sulphur crystals were found to develop within the biofilm and above a certain size these disengaged and then settled in the liquid phase below the biofilm. These features, together with the ability of the biofilm to remain suspended at the air/water interface thus provide the surface requirement, and indicate that these structures may be understood as “true” biofilms. In order to study an apparent functional differentiation within the floating sulphur biofilm system, a method was developed to expand its various components over a 13 cm length of agarose tube and across which an oxygen/sulphide gradient was established. This was done by inserting a sulphide plug in the bottom of the tube, overlaying this with the biofilm mixed and suspended in agarose and leaving the tube to open air. After allowing for growth, the different components of the microbial population occurring at various levels across the oxygen/sulphide gradient were sampled. The microbial population was found to resort in distinct functional layers. Aerobes including Acidithiobacillus and Azoarcus, Acidithiobacillus, Thiothrix, Thiovirga and Sulfurimonas were found in the upper oxidised layer. Aerobe and facultative anaerobes such as Chryseobacterium, Bacteroides and Planococcus were found in the middle and heterotrophic anaerobes such as Brevundimonas and uncultured anaerobes were found in the bottom anoxic layer. This enabled the development of a first descriptive structural/functional model accounting for the performance of floating sulphur biofilms. The potential of the floating sulphur biofilm for use as a bioprocess unit operation for sulphide removal in lignocellulose-based low-flow passive systems for acid mine drainage wastewater treatment was investigated. The linear flow channel reactor was scaled up and it was shown that the optimum sulphide removal of 74 % and sulphur recovery of 60 % could be achieved at 20 °C. In a further scale up of the linear channel reactor, the floating sulphur biofilm reactor was developed and operated. Sulphide removal and sulphur recovery of 65 and 56 % respectively was measured in the process. An understanding of the nature and function of floating sulphur biofilms and the further development of their potential application in sulphide removal in aquatic systems may provide a useful contribution to the treatment of acid mine drainage and other sulphidic wastewaters.
- Full Text:
- Date Issued: 2008
- Authors: Molwantwa, Jennifer Balatedi
- Date: 2008
- Subjects: Biofilms Sulfur Acid mine drainage -- South Africa Mine water -- Purification -- Biological treatment Microbial ecology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3958 , http://hdl.handle.net/10962/d1004017
- Description: Mine wastewaters generated during active production operations, and decanting streams following mine closure have major environmental impacts, and volumes requiring treatment are expected to increase substantially as the South African mining industry matures. Biological treatment of mine waters has been the subject of increasing interest, where sulphate reducing bacteria are employed for the reduction of sulphate to sulphide, precipitation of metals and the production of alkalinity. However, the sulphide if not removed from the system can be oxidised back to sulphate. As a result there have been limitations especially in the provision of technological options that are sustainable over the long-term, where the total sulphur (in its different forms) can be removed from the system. These, however, are the subject of a number of constraints including, importantly, the process capability to remove reduced sulphur from the treated stream, in one of its oxidation states, and thus linearise the biological sulphur cycle. This remains a major bottleneck in the development of biological wastewater treatment technology. Floating sulphur biofilms are observed as surface layers in numerous aquatic sulphide-rich environments, and it has been suggested that they play a role in the biological cycling of sulphur. The use of sulphur biofilms for the removal of elemental sulphur was identified in this study as a possible means for addressing the technological bottleneck, especially in passive wastewater treatment systems. There is, however, little documented information in the literature on the structure of floating sulphur biofilms, the microbial species responsible for their occurrence or bio-process applications of the system. A linear flow channel reactor was developed to simulate natural conditions and enabled the study of floating sulphur biofilm under controlled laboratory conditions. It was observed that these biofilms developed through three distinct stages termed Thin, Sticky and Brittle films. A microprobe study showed the presence of a steep Redox gradient established across (260 to 380 μm) depth of the floating sulphur biofilm of ~ 0 to -200 mV (top to bottom), which correlated with pH and sulphide gradients across the system. Structural investigations embedded in an exopolymeric matrix containing clearly defined channels and pores. Sulphur crystals were found to develop within the biofilm and above a certain size these disengaged and then settled in the liquid phase below the biofilm. These features, together with the ability of the biofilm to remain suspended at the air/water interface thus provide the surface requirement, and indicate that these structures may be understood as “true” biofilms. In order to study an apparent functional differentiation within the floating sulphur biofilm system, a method was developed to expand its various components over a 13 cm length of agarose tube and across which an oxygen/sulphide gradient was established. This was done by inserting a sulphide plug in the bottom of the tube, overlaying this with the biofilm mixed and suspended in agarose and leaving the tube to open air. After allowing for growth, the different components of the microbial population occurring at various levels across the oxygen/sulphide gradient were sampled. The microbial population was found to resort in distinct functional layers. Aerobes including Acidithiobacillus and Azoarcus, Acidithiobacillus, Thiothrix, Thiovirga and Sulfurimonas were found in the upper oxidised layer. Aerobe and facultative anaerobes such as Chryseobacterium, Bacteroides and Planococcus were found in the middle and heterotrophic anaerobes such as Brevundimonas and uncultured anaerobes were found in the bottom anoxic layer. This enabled the development of a first descriptive structural/functional model accounting for the performance of floating sulphur biofilms. The potential of the floating sulphur biofilm for use as a bioprocess unit operation for sulphide removal in lignocellulose-based low-flow passive systems for acid mine drainage wastewater treatment was investigated. The linear flow channel reactor was scaled up and it was shown that the optimum sulphide removal of 74 % and sulphur recovery of 60 % could be achieved at 20 °C. In a further scale up of the linear channel reactor, the floating sulphur biofilm reactor was developed and operated. Sulphide removal and sulphur recovery of 65 and 56 % respectively was measured in the process. An understanding of the nature and function of floating sulphur biofilms and the further development of their potential application in sulphide removal in aquatic systems may provide a useful contribution to the treatment of acid mine drainage and other sulphidic wastewaters.
- Full Text:
- Date Issued: 2008
Fungal remediation of winery and distillery wastewaters using Trametes pubescens MB 89 and the enhanced production of a high-value enzyme therein
- Authors: Strong, Peter James
- Date: 2008
- Subjects: Fungal remediation Distilleries -- Waste disposal Wine and wine making -- Waste disposal Bioremediation Laccase Enzymes -- Biotechnology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3932 , http://hdl.handle.net/10962/d1003991
- Description: In this study white-rot fungi were investigated for their efficiency at distillery wastewater remediation and the production of laccase as a valuable by-product. Distillery wastewaters are high in organic load and low in pH. The presence of phenolic compounds can lead to extremely colour-rich wastewaters and can be toxic to microorganisms. The presence of the inorganic ions may also affect biological treatment. White-rot fungi are unique among eukaryotic or prokaryotic microbes in possessing powerful oxidative enzyme systems that can degrade lignin to carbon dioxide. These ligninolytic enzymes, such as lignin peroxidase, manganese peroxidase and laccase, are capable of degrading a vast range of toxic, recalcitrant environmental pollutants and this makes the white-rot fungi strong candidates for the bioremediation of polluted soils and waters. The laccase enzyme alone has shown remediation potential in wastewaters such as beer production effluent, olive mill wastewater, alcohol distillery wastes, dye-containing wastewaters from the textile industry as well as wastewaters from the paper and pulp industry. It has been shown to be capable of remediating soils and waters polluted with chlorinated phenolic compounds, polyaromatic hydrocarbons, nitrosubstituted compounds and fungicides, herbicides and insecticides.
- Full Text:
- Date Issued: 2008
- Authors: Strong, Peter James
- Date: 2008
- Subjects: Fungal remediation Distilleries -- Waste disposal Wine and wine making -- Waste disposal Bioremediation Laccase Enzymes -- Biotechnology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3932 , http://hdl.handle.net/10962/d1003991
- Description: In this study white-rot fungi were investigated for their efficiency at distillery wastewater remediation and the production of laccase as a valuable by-product. Distillery wastewaters are high in organic load and low in pH. The presence of phenolic compounds can lead to extremely colour-rich wastewaters and can be toxic to microorganisms. The presence of the inorganic ions may also affect biological treatment. White-rot fungi are unique among eukaryotic or prokaryotic microbes in possessing powerful oxidative enzyme systems that can degrade lignin to carbon dioxide. These ligninolytic enzymes, such as lignin peroxidase, manganese peroxidase and laccase, are capable of degrading a vast range of toxic, recalcitrant environmental pollutants and this makes the white-rot fungi strong candidates for the bioremediation of polluted soils and waters. The laccase enzyme alone has shown remediation potential in wastewaters such as beer production effluent, olive mill wastewater, alcohol distillery wastes, dye-containing wastewaters from the textile industry as well as wastewaters from the paper and pulp industry. It has been shown to be capable of remediating soils and waters polluted with chlorinated phenolic compounds, polyaromatic hydrocarbons, nitrosubstituted compounds and fungicides, herbicides and insecticides.
- Full Text:
- Date Issued: 2008
Harvesting strategies of fuelwood and kraalwood users at Machibi : identifying the driving factors and feedbacks
- Authors: Scheepers, Kelly
- Date: 2008
- Subjects: Fuelwood -- South Africa -- Eastern Cape Forest ecology -- South Africa -- Eastern Cape Natural resources -- South Africa -- Eastern Cape Natural resources -- Management -- South Africa -- Eastern Cape Conservation of natural resources -- South Africa -- Eastern Cape Landscape protection -- Economic aspects -- South Africa -- Eastern Cape Fuelwood consumption -- South Africa -- Eastern Cape Fuelwood consumption -- Economic aspects -- South Africa -- Eastern Cape Forests and forestry -- South Africa -- Eastern Cape Fuelwood conservation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4765 , http://hdl.handle.net/10962/d1007167
- Description: Forest and woodland ecosystems provide a variety of natural resources such as fuelwood, brushwood and kraal posts to local communities, as well as possess important cultural and spiritual value. However, many forests and woodlands worldwide have been unsustainably used and managed. Thus, under pressure from the international conservation community to recognise the importance of people's relationships with their surrounding natural environment, particularly for the natural resources it can provide, and given a move away from the management of forests and woodlands for sustained yields, and according to simple cause and effect models, in favour of systems approaches, South Africa has developed some of the most progressive natural resource management policies in the world. Nevertheless, for these policies to be sensitive to local contexts, there remains a need for a better understanding of how local people in different contexts, determine forest and woodland ecosystems to be of use to them, and what 'usefulness' means to different groups of resources users. This is a case study, which examines the role of fuelwood, brushwood and kraal posts in the rural livelihoods of the people of Machibi village, located in the Eastern Cape province of South Africa, through people's preferences for particular landscapes and species, accessed for these purposes, and the trade-offs people make between resource availability and resource accessibility. Key objectives of the study are to 1) determine the preferred landscapes and species for fuelwood, brushwood and kraal posts at Machibi, 2) determine the landscapes and species actually used for fuelwood, brushwood and kraal posts, and 3) with the help of a conceptual model, and using iterative modelling as a tool, determine the factors that influence people's harvesting strategies in terms of the costs and benefits associated with the different landscape and species options. On the basis of this knowledge, the study provides some guiding principles for the better use and management of these landscapes and species for fuelwood, brushwood and kraal posts. An innovative research approach and methodology that integrates social and ecological systems, works across disciplines, and draws on different types of knowledge is used to develop and test a conceptual model of the harvesting strategies of fuelwood and kraalwood users at Machibi. Participatory methods such as workshops, participatory resource mapping, ranking exercises and trend-lines were used to tap into local knowledge while plotless vegetation sampling and GIS maps were used to capture the scientific information. Results showed that people did not always use the landscapes and species they preferred. However, the local people did behave in a rational manner by weighing up the returns from harvesting and accessibility costs associated with the respective options available to them, before selecting the option(s) associated with the greatest net benefits. At the landscape level, people made trade-offs between the returns from harvesting and the accessibility costs of using particular landscapes in addition to costs associated with the physical work of harvesting fuelwood, brushwood or kraal posts from these landscapes. At the species level, people made trade-offs between the returns from harvesting and the accessibility costs of harvesting particular species for fuelwood, brushwood and kraal posts, or the costs of commercial alternatives. Costbenefit factors that influenced people's resource use patterns also differed across landscapes and species for fuelwood, brushwood and kraal posts, respectively. Consequently, a range of diverse and flexible management options and strategies is recommended for the wise use and management of these landscapes and species, focused on short, medium and long term goals. These strategies examine the use of cost - benefit incentives to influence people’s landscape and species use patterns.
- Full Text:
- Date Issued: 2008
- Authors: Scheepers, Kelly
- Date: 2008
- Subjects: Fuelwood -- South Africa -- Eastern Cape Forest ecology -- South Africa -- Eastern Cape Natural resources -- South Africa -- Eastern Cape Natural resources -- Management -- South Africa -- Eastern Cape Conservation of natural resources -- South Africa -- Eastern Cape Landscape protection -- Economic aspects -- South Africa -- Eastern Cape Fuelwood consumption -- South Africa -- Eastern Cape Fuelwood consumption -- Economic aspects -- South Africa -- Eastern Cape Forests and forestry -- South Africa -- Eastern Cape Fuelwood conservation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4765 , http://hdl.handle.net/10962/d1007167
- Description: Forest and woodland ecosystems provide a variety of natural resources such as fuelwood, brushwood and kraal posts to local communities, as well as possess important cultural and spiritual value. However, many forests and woodlands worldwide have been unsustainably used and managed. Thus, under pressure from the international conservation community to recognise the importance of people's relationships with their surrounding natural environment, particularly for the natural resources it can provide, and given a move away from the management of forests and woodlands for sustained yields, and according to simple cause and effect models, in favour of systems approaches, South Africa has developed some of the most progressive natural resource management policies in the world. Nevertheless, for these policies to be sensitive to local contexts, there remains a need for a better understanding of how local people in different contexts, determine forest and woodland ecosystems to be of use to them, and what 'usefulness' means to different groups of resources users. This is a case study, which examines the role of fuelwood, brushwood and kraal posts in the rural livelihoods of the people of Machibi village, located in the Eastern Cape province of South Africa, through people's preferences for particular landscapes and species, accessed for these purposes, and the trade-offs people make between resource availability and resource accessibility. Key objectives of the study are to 1) determine the preferred landscapes and species for fuelwood, brushwood and kraal posts at Machibi, 2) determine the landscapes and species actually used for fuelwood, brushwood and kraal posts, and 3) with the help of a conceptual model, and using iterative modelling as a tool, determine the factors that influence people's harvesting strategies in terms of the costs and benefits associated with the different landscape and species options. On the basis of this knowledge, the study provides some guiding principles for the better use and management of these landscapes and species for fuelwood, brushwood and kraal posts. An innovative research approach and methodology that integrates social and ecological systems, works across disciplines, and draws on different types of knowledge is used to develop and test a conceptual model of the harvesting strategies of fuelwood and kraalwood users at Machibi. Participatory methods such as workshops, participatory resource mapping, ranking exercises and trend-lines were used to tap into local knowledge while plotless vegetation sampling and GIS maps were used to capture the scientific information. Results showed that people did not always use the landscapes and species they preferred. However, the local people did behave in a rational manner by weighing up the returns from harvesting and accessibility costs associated with the respective options available to them, before selecting the option(s) associated with the greatest net benefits. At the landscape level, people made trade-offs between the returns from harvesting and the accessibility costs of using particular landscapes in addition to costs associated with the physical work of harvesting fuelwood, brushwood or kraal posts from these landscapes. At the species level, people made trade-offs between the returns from harvesting and the accessibility costs of harvesting particular species for fuelwood, brushwood and kraal posts, or the costs of commercial alternatives. Costbenefit factors that influenced people's resource use patterns also differed across landscapes and species for fuelwood, brushwood and kraal posts, respectively. Consequently, a range of diverse and flexible management options and strategies is recommended for the wise use and management of these landscapes and species, focused on short, medium and long term goals. These strategies examine the use of cost - benefit incentives to influence people’s landscape and species use patterns.
- Full Text:
- Date Issued: 2008
Identification and characterization of novel oncology related platinum complexes using chromatographic and mass spectrometric techniques
- Authors: Wentzel, Mauritz
- Date: 2008
- Subjects: Chromatographic analysis , Spectrum analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10310 , http://hdl.handle.net/10948/715 , Chromatographic analysis , Spectrum analysis
- Description: In this thesis mass spectral and chromatographic techniques were developed and applied to identify and characterise numerous novel platinum(II) and (IV) compounds designed as anticancer agents. In a novel method for the synthesis of cis-oxalato(trans- -1,2- cyclohexanediamine)platinum(II) or oxaliplatin these techniques could be applied to differentiate between the molecular complex and the autoionised analogue (viz. Ptdach2 2+Ptox2 2-). In another novel synthetic method for the same compound the ligand exchange reactions at various temperatures could be investigated and kinetic curves obtained served to illuminate the chemistry involved, indicating the role of small amounts of water in the essentially non-aqueous solvent systems dmf and isoamyl alcohol respectively. These allowed ligand exchange without resulting in hydrolyses even up to 85°C. The ionisation rate of divalent platinum halide complexes was determined for various amine ligands as well as N-S chelate ligands. A comparison of these could suggest why N-S complexes have poor anticancer action. Ionisation was not only studied for neutral molecular species but also for monocationic ones. Relationships could be found with stereochemical aspects of the chelates used. By investigating results of EV-CAD studies thermodynamic data could be obtained which indicated that bond strength decreases from chloro to iodo analogues although extent of ionisation in aqueous solution, i.e kinetic stability, is the reverse. Products formed by the reaction of NO2 gas with Platinum(II) compounds could be identified and separated which greatly contributed to the understanding of the chemistry involved in the formation of mononitro platinum(IV) complexes. Some of these proved to have exceptional anticancer properties. Studies of the interaction of thiol containing biomolecules were performed as a function of time. The results contributed to the understanding of the action of the anticancer agents.
- Full Text:
- Date Issued: 2008
- Authors: Wentzel, Mauritz
- Date: 2008
- Subjects: Chromatographic analysis , Spectrum analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10310 , http://hdl.handle.net/10948/715 , Chromatographic analysis , Spectrum analysis
- Description: In this thesis mass spectral and chromatographic techniques were developed and applied to identify and characterise numerous novel platinum(II) and (IV) compounds designed as anticancer agents. In a novel method for the synthesis of cis-oxalato(trans- -1,2- cyclohexanediamine)platinum(II) or oxaliplatin these techniques could be applied to differentiate between the molecular complex and the autoionised analogue (viz. Ptdach2 2+Ptox2 2-). In another novel synthetic method for the same compound the ligand exchange reactions at various temperatures could be investigated and kinetic curves obtained served to illuminate the chemistry involved, indicating the role of small amounts of water in the essentially non-aqueous solvent systems dmf and isoamyl alcohol respectively. These allowed ligand exchange without resulting in hydrolyses even up to 85°C. The ionisation rate of divalent platinum halide complexes was determined for various amine ligands as well as N-S chelate ligands. A comparison of these could suggest why N-S complexes have poor anticancer action. Ionisation was not only studied for neutral molecular species but also for monocationic ones. Relationships could be found with stereochemical aspects of the chelates used. By investigating results of EV-CAD studies thermodynamic data could be obtained which indicated that bond strength decreases from chloro to iodo analogues although extent of ionisation in aqueous solution, i.e kinetic stability, is the reverse. Products formed by the reaction of NO2 gas with Platinum(II) compounds could be identified and separated which greatly contributed to the understanding of the chemistry involved in the formation of mononitro platinum(IV) complexes. Some of these proved to have exceptional anticancer properties. Studies of the interaction of thiol containing biomolecules were performed as a function of time. The results contributed to the understanding of the action of the anticancer agents.
- Full Text:
- Date Issued: 2008