The metabolism of abscisic acid in higher plant tissues
- Authors: Cowan, Ashton Keith
- Date: 1989
- Subjects: Plants -- Metabolism Plant cells and tissues Abscisic acid -- Metabolism
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4176 , http://hdl.handle.net/10962/d1002024
- Description: The biosynthesis of ABA from R-[2-¹⁴C]-MVA was demonstrated in Persea americana cv. Fuerte mesocarp and in mature seeds of Hordeum vulgare cv. Dyan and cv. Himalaya. Radioactivity from R-[2-¹⁴-C]-MVA was also incorporated into the 1',4'-trans ABA diol in Persea americana mesocarp and a possible role for this metabolite as a precursor of ABA in plants is discussed. The biosynthesis of ABA from MVA could not be demonstrated in either turgid and waterstressed Hordeum vulgare cv. Dyan, Pisum sativum cv. Black-eyed Susan and Phaseolus vulgaris cv. Top-crop or in immature seeds of Pisum sativum and Phaseolus vulgaris. (R,S,)-[2-¹⁴C]-ABA was catabolised to PA, DPA and aqueous conjugates in leaves and mature seeds of Hordeum vulgare cv. Dyan, seedlings and immature seeds of Pisum sativum and Phaseolus vulgaris and in mesocarp from ripening fruits of Persea americana. PA and DPA were identified by either microchemical methods and/or capillary GC-MS. 7'-Hydroxy ABA was characterised as a novel ABA catabolite in light-grown and etiolated leaves of Hordeum vulgare by capillary GC-MS. Circular dichroism analysis revealed that it was derived predominantly from the (R)-enantiomer of ABA. This catabolite was absent in similar studies using the dicotyledons Pisum sativum and Phaseolus vulgaris. Refeeding studies with [¹⁴C]-PA, [C]-DPA and [¹⁴C]-7'-hydroxy ABA were used to confirm the metabolic interrelationship between ABA and its catabolites in both vegetative and non-vegetative tissues from monocotyledonous and dicotyledonous species. The methyl ester of (R,S,)-ABA was hydrolysed efficiently by light-grown leaves of Hordeum vulgare. Older, vegetative tissues catabolised (R,S,)-ABA more efficiently than their younger counterparts. In contrast, small, immature seeds of Pisum sativum catabolised (R,S,)-ABA more effectively than larger, immature seeds of this species. Light did not appear to influence ABA biosynthesis but markedly enhanced ABA catabolism. Light stimulated the overall rate of ABA catabolism in both vegetative and non-vegetative tissue. Water stress reduced ABA catabolism in Hordeum vulgare leaves but had little effect on this process in Phaseolus vulgaris seedlings. Pretreatment of tissues with (R,S,)-ABA retarded the catabolism of (R,S,)-[2-¹⁴C]-ABA, negating ABA-induced conversion to PA. Cycloheximide inhibited ABA biosynthesis and catabolism but did not affect ABA conjugation. Chloramphenicol and lincomycin had little or no effect on ABA metabolism suggesting that the enzymes involved were labile and cytoplasmic in origin. Ancymidol and cycocel inhibited ABA biosynthesis while AM01618 stimulated this process. The cytokinins, benzyladenine, kinetin, isopentenyl adenine and zeatin also inhibited ABA biosynthesis. These results are discussed in relation to the possible involvement of carotenoids in ABA biosynthesis. AM01618, ancymidol andcycocel did not significantly influence the conversion of ABA to PA and DPA while cytokinins appeared to enhance this process only in vegetative tissue. The information derived from these studies was then used in attempts to develop a cell-free system from higher plants capable of metabolising ABA. A cell-free system prepared from imbibed Hordeum vulgare cv. Dyan embryos biosynthesized and catabolised ABA. This is the first demonstration of a cell-free system from non-vegetative tissue capable of metabolising ABA and could prove useful for elucidating its biosynthetic route. This cell-free system generated the terpenyl pyrophosphates IPP, FPP and GGPP from MVA. ABA was produced from both MVA and IPP in the presence of 0₂ and NADPH. The biosynthesis of ABA was stimulated by the addition of the squalene 2,3-oxide cyclase and kaurene synthetase inhibitor, AM01618 and a "cold-pool trap" of (R,S,)-ABA. Ancymidol, cycocel and cytokinins reduced incorporation of label from MVA into ABA. Similar cell-free preparations, in the absence of AM01618, converted (R,S,)-[2-¹⁴-C]-ABA into PA, 7'-hydroxy ABA and water-soluble conjugates. Although the methyl ester of (R,S,)-ABA was efficiently hydrolysed in this cell-free system no DPA was generated. The possible involvement of mixed function oxidase activity and soluble oxidases is discussed in relation to ABA metabolism. While cell-free preparations from Persea americana cv. Fuerte mesocarp and immature seeds of Pisum sativum and Phaseolus vulgaris were unable to synthesize ABA from MVA, these tissue homogenates converted ABA into more polar acidic products. PA and DPA were identified as products of ABA catabolism in extracts from immature seeds of Phaseolus vulgaris and the l',4'-cis diol of ABA in extracts from Pisum sativum immature seeds
- Full Text:
- Date Issued: 1989
- Authors: Cowan, Ashton Keith
- Date: 1989
- Subjects: Plants -- Metabolism Plant cells and tissues Abscisic acid -- Metabolism
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4176 , http://hdl.handle.net/10962/d1002024
- Description: The biosynthesis of ABA from R-[2-¹⁴C]-MVA was demonstrated in Persea americana cv. Fuerte mesocarp and in mature seeds of Hordeum vulgare cv. Dyan and cv. Himalaya. Radioactivity from R-[2-¹⁴-C]-MVA was also incorporated into the 1',4'-trans ABA diol in Persea americana mesocarp and a possible role for this metabolite as a precursor of ABA in plants is discussed. The biosynthesis of ABA from MVA could not be demonstrated in either turgid and waterstressed Hordeum vulgare cv. Dyan, Pisum sativum cv. Black-eyed Susan and Phaseolus vulgaris cv. Top-crop or in immature seeds of Pisum sativum and Phaseolus vulgaris. (R,S,)-[2-¹⁴C]-ABA was catabolised to PA, DPA and aqueous conjugates in leaves and mature seeds of Hordeum vulgare cv. Dyan, seedlings and immature seeds of Pisum sativum and Phaseolus vulgaris and in mesocarp from ripening fruits of Persea americana. PA and DPA were identified by either microchemical methods and/or capillary GC-MS. 7'-Hydroxy ABA was characterised as a novel ABA catabolite in light-grown and etiolated leaves of Hordeum vulgare by capillary GC-MS. Circular dichroism analysis revealed that it was derived predominantly from the (R)-enantiomer of ABA. This catabolite was absent in similar studies using the dicotyledons Pisum sativum and Phaseolus vulgaris. Refeeding studies with [¹⁴C]-PA, [C]-DPA and [¹⁴C]-7'-hydroxy ABA were used to confirm the metabolic interrelationship between ABA and its catabolites in both vegetative and non-vegetative tissues from monocotyledonous and dicotyledonous species. The methyl ester of (R,S,)-ABA was hydrolysed efficiently by light-grown leaves of Hordeum vulgare. Older, vegetative tissues catabolised (R,S,)-ABA more efficiently than their younger counterparts. In contrast, small, immature seeds of Pisum sativum catabolised (R,S,)-ABA more effectively than larger, immature seeds of this species. Light did not appear to influence ABA biosynthesis but markedly enhanced ABA catabolism. Light stimulated the overall rate of ABA catabolism in both vegetative and non-vegetative tissue. Water stress reduced ABA catabolism in Hordeum vulgare leaves but had little effect on this process in Phaseolus vulgaris seedlings. Pretreatment of tissues with (R,S,)-ABA retarded the catabolism of (R,S,)-[2-¹⁴C]-ABA, negating ABA-induced conversion to PA. Cycloheximide inhibited ABA biosynthesis and catabolism but did not affect ABA conjugation. Chloramphenicol and lincomycin had little or no effect on ABA metabolism suggesting that the enzymes involved were labile and cytoplasmic in origin. Ancymidol and cycocel inhibited ABA biosynthesis while AM01618 stimulated this process. The cytokinins, benzyladenine, kinetin, isopentenyl adenine and zeatin also inhibited ABA biosynthesis. These results are discussed in relation to the possible involvement of carotenoids in ABA biosynthesis. AM01618, ancymidol andcycocel did not significantly influence the conversion of ABA to PA and DPA while cytokinins appeared to enhance this process only in vegetative tissue. The information derived from these studies was then used in attempts to develop a cell-free system from higher plants capable of metabolising ABA. A cell-free system prepared from imbibed Hordeum vulgare cv. Dyan embryos biosynthesized and catabolised ABA. This is the first demonstration of a cell-free system from non-vegetative tissue capable of metabolising ABA and could prove useful for elucidating its biosynthetic route. This cell-free system generated the terpenyl pyrophosphates IPP, FPP and GGPP from MVA. ABA was produced from both MVA and IPP in the presence of 0₂ and NADPH. The biosynthesis of ABA was stimulated by the addition of the squalene 2,3-oxide cyclase and kaurene synthetase inhibitor, AM01618 and a "cold-pool trap" of (R,S,)-ABA. Ancymidol, cycocel and cytokinins reduced incorporation of label from MVA into ABA. Similar cell-free preparations, in the absence of AM01618, converted (R,S,)-[2-¹⁴-C]-ABA into PA, 7'-hydroxy ABA and water-soluble conjugates. Although the methyl ester of (R,S,)-ABA was efficiently hydrolysed in this cell-free system no DPA was generated. The possible involvement of mixed function oxidase activity and soluble oxidases is discussed in relation to ABA metabolism. While cell-free preparations from Persea americana cv. Fuerte mesocarp and immature seeds of Pisum sativum and Phaseolus vulgaris were unable to synthesize ABA from MVA, these tissue homogenates converted ABA into more polar acidic products. PA and DPA were identified as products of ABA catabolism in extracts from immature seeds of Phaseolus vulgaris and the l',4'-cis diol of ABA in extracts from Pisum sativum immature seeds
- Full Text:
- Date Issued: 1989
The thermodynamics of binary liquid mixtures of compounds containing multiple bonds.
- Authors: Baxter, Rodney Charles
- Date: 1989
- Subjects: Solution (Chemistry)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4531 , http://hdl.handle.net/10962/d1016079
- Description: Excess thermodynamic properties have been determined for several binary liquid mixtures with the aim of testing various thermodynamic theories and postulates. Excess molar enthalpies, HEm, have been determined using an LKB flow microcalorimeter and excess molar volumes, VEm, have been determined using an Anton Paar vibrating tube densitometer. The activity coefficients at infinite dilution ƴ∞₁₃, have been determined using an atmospheric pressure gas-liquid chromatograph. The excess molar enthalpies and the excess molar volumes have been measured at 298.15 K for systems involving the bicyclic compounds decahydronaphthalene (decalin), 1,2,3,4-tetrahydronaphthalene (tetralin), bicyclohexyl, or cyclohexylbenzene mixed with 1- hexene, 1-hexyne, 1-heptene, 1-heptyne, cyclohexene, 1,3-cyclohexadiene, 1,4- cyclohexadiene, or benzene. These excess properties have also been measured for systems where the bicyclic compound has been replaced with benzene, cyclohexane or n-hexane. The results show defmite trends related to the size, shape, and the degree of unsaturation of the component molecules. The Flory theory has been used to predict excess molar enthalpies and excess molar volumes for {(a bicyclic compound or benzene or cyclohexane or n-hexane) +(an n-alkane or a 1-alkene or a 1-alkyne or a cycloalkane or cyclohexene or a cycloalkadiene or benzene)}. The one parameter equations offer reasonably good correlations between the predicted and the experimental results. More insight into the origins of the contnbutions to the excess thermodynamic properties for these systems has been gained by considering the approximate equations of Patterson and co-workers, which separate the interactional and the free volume contributions to the excess molar enthalpy and the excess molar volume. The one parameter equations have adequately rationalized a good deal of the observed behaviour for HEm and VEm. The theory of Liebermann and co-workers, which does not employ any adjustable parameters, has not been as successful at predicting the excess thermodynamic properties for the above systems. The activity coefficients at infinite dilution have been measured at 278.15 K, 288.15 K and 298.15 K for n-bexane, 1-bexene, 1-hexyne, n-heptane, 1-heptene, 1-heptyne, cyclohexane, cyclohexene, 1,3-cyclohexadiene, 1,4-cyclohexadiene, and benzene, in decalin, tetralin, bicyclohexyl, and cyclohexylbenzene. Solvent losses from the column have been accounted for by an extrapolation procedure. The activity coefficient results together with the HEm and VEm values have been used to calculate the partial molar excess thermodynamic properties of mixing at infinite dilution. The partial molar excess properties at infinite dilution for decalin mixtures are similar to those for bicyclohexyl mixtures. There is also a similarity between the properties of the tetralin mixtures and the cyclohexylbenzene mixtures. The cycloalkadienes, benzene and the 1-alkynes exhibit a strong dissociation effect on being mixed with the saturated solvents, decalin and bicyclohexyl, but associate strongly with tetralin and with cyclohexylbenzene. The Flory theory bas been used to predict activity coefficients at infinite dilution from the experimentally determined HEm results for { (n-bexane or 1-hexene or 1-hexyne or naheptane or 1-heptene or 1-beptyne) + (a bicyclic compound)}. The theory is much better at predicting values for mixtures where both components are either saturated molecules or are unsaturated molecules than for {saturated + unsaturated} mixtures.
- Full Text:
- Date Issued: 1989
- Authors: Baxter, Rodney Charles
- Date: 1989
- Subjects: Solution (Chemistry)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4531 , http://hdl.handle.net/10962/d1016079
- Description: Excess thermodynamic properties have been determined for several binary liquid mixtures with the aim of testing various thermodynamic theories and postulates. Excess molar enthalpies, HEm, have been determined using an LKB flow microcalorimeter and excess molar volumes, VEm, have been determined using an Anton Paar vibrating tube densitometer. The activity coefficients at infinite dilution ƴ∞₁₃, have been determined using an atmospheric pressure gas-liquid chromatograph. The excess molar enthalpies and the excess molar volumes have been measured at 298.15 K for systems involving the bicyclic compounds decahydronaphthalene (decalin), 1,2,3,4-tetrahydronaphthalene (tetralin), bicyclohexyl, or cyclohexylbenzene mixed with 1- hexene, 1-hexyne, 1-heptene, 1-heptyne, cyclohexene, 1,3-cyclohexadiene, 1,4- cyclohexadiene, or benzene. These excess properties have also been measured for systems where the bicyclic compound has been replaced with benzene, cyclohexane or n-hexane. The results show defmite trends related to the size, shape, and the degree of unsaturation of the component molecules. The Flory theory has been used to predict excess molar enthalpies and excess molar volumes for {(a bicyclic compound or benzene or cyclohexane or n-hexane) +(an n-alkane or a 1-alkene or a 1-alkyne or a cycloalkane or cyclohexene or a cycloalkadiene or benzene)}. The one parameter equations offer reasonably good correlations between the predicted and the experimental results. More insight into the origins of the contnbutions to the excess thermodynamic properties for these systems has been gained by considering the approximate equations of Patterson and co-workers, which separate the interactional and the free volume contributions to the excess molar enthalpy and the excess molar volume. The one parameter equations have adequately rationalized a good deal of the observed behaviour for HEm and VEm. The theory of Liebermann and co-workers, which does not employ any adjustable parameters, has not been as successful at predicting the excess thermodynamic properties for the above systems. The activity coefficients at infinite dilution have been measured at 278.15 K, 288.15 K and 298.15 K for n-bexane, 1-bexene, 1-hexyne, n-heptane, 1-heptene, 1-heptyne, cyclohexane, cyclohexene, 1,3-cyclohexadiene, 1,4-cyclohexadiene, and benzene, in decalin, tetralin, bicyclohexyl, and cyclohexylbenzene. Solvent losses from the column have been accounted for by an extrapolation procedure. The activity coefficient results together with the HEm and VEm values have been used to calculate the partial molar excess thermodynamic properties of mixing at infinite dilution. The partial molar excess properties at infinite dilution for decalin mixtures are similar to those for bicyclohexyl mixtures. There is also a similarity between the properties of the tetralin mixtures and the cyclohexylbenzene mixtures. The cycloalkadienes, benzene and the 1-alkynes exhibit a strong dissociation effect on being mixed with the saturated solvents, decalin and bicyclohexyl, but associate strongly with tetralin and with cyclohexylbenzene. The Flory theory bas been used to predict activity coefficients at infinite dilution from the experimentally determined HEm results for { (n-bexane or 1-hexene or 1-hexyne or naheptane or 1-heptene or 1-beptyne) + (a bicyclic compound)}. The theory is much better at predicting values for mixtures where both components are either saturated molecules or are unsaturated molecules than for {saturated + unsaturated} mixtures.
- Full Text:
- Date Issued: 1989
Towards an existential phenomenological interpretation of C.G. Jung's analytical psychology
- Authors: Brooke, Roger, 1953-
- Date: 1989
- Subjects: Jung, C G (Carl Gustav), 1875-1961 Existential phenomenology Phenomenology Psychoanalysis Psychology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3208 , http://hdl.handle.net/10962/d1011983
- Description: The central aim of this study was to interpret the psychology of C.G . Jung in the light of existential phenomenology, thereby to lay the foundations for an integrated phenomenological analytical psychology. It was recognised that although Jung introduced a poetic understanding of psychological life he tended to adhere theoretically to a Cartesian and natural scientific epistemology and ontology, in which the knower is separated from the known, and psychological life is encapsulated inside the human subject. Thus the main task, which defined generally the study's scope and limitations, was to undercut the lingering Cartesianism in Jung's thought, thereby to recover the world in which one lives as intrinsically and authentically psychological, and one's psychological life as irreducibly world-related. The ontological guidelines for this endeavour were taken primarily from Heidegger and Merleau-Ponty, but it was consistently argued that this hermeneutic movement towards an existential understanding is given within Jung's work itself. Thus: Jung's method is primarily hermeneutic-phenomenological; the psyche is not "mind" or an inner realm more or less linked to the body, but is the embodied life-world, and Jung's descriptions of it - of its autonomy, spatiality and bodiliness, for instance - achieve ontological clarity when it is articulated as Dasein; the self as the totality of the psyche is interpretated in terms of Dasein, and individuation involves differentiation, personalisation and appropriation within existence itself; the complexes, archetypally grounded, are the vital densities of incarnate life, ambiguously conscious and unconscious, known and lived; the archetypes are the fundamental necessities of psychological life, autonomous imaginal structures within which both body and world are founded. Imaginal autonomy is revealed ontically as the metaphorical reality of things, but since imaginal autonomy has no ground thought about psychological life is ultimately poetic. Where relevant, recent theoretical developments in analytical psychology were discussed, particularly the Developmental and Archetypal movements. A clinical study was presented to illustrate some of the main themes of the thesis. In conclusion, the main themes of an integrated phenomenological analytical psychology were outlined, and the central contributions of analytical psychology and existential phenomenology were highlighted.
- Full Text:
- Date Issued: 1989
- Authors: Brooke, Roger, 1953-
- Date: 1989
- Subjects: Jung, C G (Carl Gustav), 1875-1961 Existential phenomenology Phenomenology Psychoanalysis Psychology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3208 , http://hdl.handle.net/10962/d1011983
- Description: The central aim of this study was to interpret the psychology of C.G . Jung in the light of existential phenomenology, thereby to lay the foundations for an integrated phenomenological analytical psychology. It was recognised that although Jung introduced a poetic understanding of psychological life he tended to adhere theoretically to a Cartesian and natural scientific epistemology and ontology, in which the knower is separated from the known, and psychological life is encapsulated inside the human subject. Thus the main task, which defined generally the study's scope and limitations, was to undercut the lingering Cartesianism in Jung's thought, thereby to recover the world in which one lives as intrinsically and authentically psychological, and one's psychological life as irreducibly world-related. The ontological guidelines for this endeavour were taken primarily from Heidegger and Merleau-Ponty, but it was consistently argued that this hermeneutic movement towards an existential understanding is given within Jung's work itself. Thus: Jung's method is primarily hermeneutic-phenomenological; the psyche is not "mind" or an inner realm more or less linked to the body, but is the embodied life-world, and Jung's descriptions of it - of its autonomy, spatiality and bodiliness, for instance - achieve ontological clarity when it is articulated as Dasein; the self as the totality of the psyche is interpretated in terms of Dasein, and individuation involves differentiation, personalisation and appropriation within existence itself; the complexes, archetypally grounded, are the vital densities of incarnate life, ambiguously conscious and unconscious, known and lived; the archetypes are the fundamental necessities of psychological life, autonomous imaginal structures within which both body and world are founded. Imaginal autonomy is revealed ontically as the metaphorical reality of things, but since imaginal autonomy has no ground thought about psychological life is ultimately poetic. Where relevant, recent theoretical developments in analytical psychology were discussed, particularly the Developmental and Archetypal movements. A clinical study was presented to illustrate some of the main themes of the thesis. In conclusion, the main themes of an integrated phenomenological analytical psychology were outlined, and the central contributions of analytical psychology and existential phenomenology were highlighted.
- Full Text:
- Date Issued: 1989
Vegetation ecology of the Camdebo and Sneeuberg regions of the Karoo biome, South Africa
- Authors: Palmer, Anthony Riordan
- Date: 1989
- Subjects: Plants -- South Africa -- Great Karoo Great Karoo (South Africa)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4173 , http://hdl.handle.net/10962/d1002021
- Description: An hierarchical syntaxonomic classification of the vegetation of the Camdebo and Sneeuberg regions of the karoo biome is presented as a second approximation after the earlier work by Acocks (1953). Details on the geomorphology, geology, climate, and early vegetation history of the area are given. The vegetation of the study area was stratified with the aid of Landsat imagery and the community classification was generated using two-way indicator species analysis (Twins pan) which produced ordered phytosociological tables. Tabular comparisons and final sorting of tables are according to the methods and techniques of the ZiirichMontpellier school of phytosociology. Syntaxonomic ranks are defined as five classes, nine orders and seventeen communities. The classes are Grasslands, Karoo Shrublands, Karoo Dwarf Shrublands, Sub-tropical Transitional Thicket, and Riparian Thicket. The distribution of syntaxa corresponds with the steep precipitation gradient experienced in the study area. These vegetation concepts are applied to the description of the flora of the Karoo Nature Reserve and an analysis of the total flora of the reserve is provided. The communities of the pediments, which contain the highest number of endemics, are poorly conserved. I test the validity of the vegetation classification by interpreting the results of an analysis of soils within the hypothesized vegetation units. There is a gradient of increasing Na, silt and pH levels from the Shrublands and Grasslands to the Succulent and Grassy Dwarf Shrublands of the pediments. A qualitative model of the vegetation history during the glacial-interglacial sequence in the Graaff-Reinet region of the eastern Cape is presented. Using a descriptive approach, the distribution patterns of 68 taxa, which are differential species for Karoo Shrublands, Succulent Thicket and Karoo Dwarf Shrublands, are investigated relative to major southern African biomes. The results indicate that a large proportion of the differential species in the phytosociological classification show affinity with Grassland and Savanna Biomes. Three species groups encountered in the Dwarf Shrublands show affinity with the Nama-Karoo biome. The differential species of the Succulent Thicket have a predominantly subtropical distribution. Using an historical approach, the palaeoenvironment of the region during the past 20 000 years is discussed briefly. On the basis of the descriptive and historical perspectives, a qualitative model of vegetation history is presented. The Succulent Thicket may have become established on edaphically favourable sites in the ameliorating conditions of the warmer, wetter Holocene subsequent to the Last Glacial Maximum. The Dwarf Shrubland and Succulent Dwarf Shrubland are depauperate in relation to ccmmunities in other southern African biomes, but the relatively large number of endemics suggests a long history in the region. Their differential species groups occur under arid conditions, accompanied by soils with high base and fertility status. The Dwarf Shrublands may have been more extensive during the drier glacial times on those sites currently occupied by Shrubland. The Shrublands display the expected affInity with the Grassland and Savanna Biomes. The small number of endemics suggest that these communities may have occupied the region in the period since the Last Glacial Maximum. Species with Succulent Karoo Biome affInity are poorly represented. The reliability of using Landsat products to detect and map the vegetation of the region is assessed. The manual classification of Landsat standard products provides a poor reflection of the vegetation of the arid, sparsely-vegetated bottomlands and pediments. The products provide good representation of the boundaries of thicket vegetation, but this uni-temporal approach does not distinguish between floristically different thicket communities. After analyzing digital Landsat data, I suggest that the multi-spectral scanner detects the boundaries of broad soil pedons and geological formations in areas of low vegetative cover. I describe and map the vegetation categories of the region after manual interpretation of six Landsat scenes. This is an effIcient, cost-effective method of mapping vegetation in extensive regions. The mapping units do not reflect the syntaxonomic classification, representing rather an integration of physiographic, pedological, geological and floristic information. With the view to improving the classification of these units, I develop a qualitative model of the natural resources of the region using an expert system
- Full Text:
- Date Issued: 1989
- Authors: Palmer, Anthony Riordan
- Date: 1989
- Subjects: Plants -- South Africa -- Great Karoo Great Karoo (South Africa)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4173 , http://hdl.handle.net/10962/d1002021
- Description: An hierarchical syntaxonomic classification of the vegetation of the Camdebo and Sneeuberg regions of the karoo biome is presented as a second approximation after the earlier work by Acocks (1953). Details on the geomorphology, geology, climate, and early vegetation history of the area are given. The vegetation of the study area was stratified with the aid of Landsat imagery and the community classification was generated using two-way indicator species analysis (Twins pan) which produced ordered phytosociological tables. Tabular comparisons and final sorting of tables are according to the methods and techniques of the ZiirichMontpellier school of phytosociology. Syntaxonomic ranks are defined as five classes, nine orders and seventeen communities. The classes are Grasslands, Karoo Shrublands, Karoo Dwarf Shrublands, Sub-tropical Transitional Thicket, and Riparian Thicket. The distribution of syntaxa corresponds with the steep precipitation gradient experienced in the study area. These vegetation concepts are applied to the description of the flora of the Karoo Nature Reserve and an analysis of the total flora of the reserve is provided. The communities of the pediments, which contain the highest number of endemics, are poorly conserved. I test the validity of the vegetation classification by interpreting the results of an analysis of soils within the hypothesized vegetation units. There is a gradient of increasing Na, silt and pH levels from the Shrublands and Grasslands to the Succulent and Grassy Dwarf Shrublands of the pediments. A qualitative model of the vegetation history during the glacial-interglacial sequence in the Graaff-Reinet region of the eastern Cape is presented. Using a descriptive approach, the distribution patterns of 68 taxa, which are differential species for Karoo Shrublands, Succulent Thicket and Karoo Dwarf Shrublands, are investigated relative to major southern African biomes. The results indicate that a large proportion of the differential species in the phytosociological classification show affinity with Grassland and Savanna Biomes. Three species groups encountered in the Dwarf Shrublands show affinity with the Nama-Karoo biome. The differential species of the Succulent Thicket have a predominantly subtropical distribution. Using an historical approach, the palaeoenvironment of the region during the past 20 000 years is discussed briefly. On the basis of the descriptive and historical perspectives, a qualitative model of vegetation history is presented. The Succulent Thicket may have become established on edaphically favourable sites in the ameliorating conditions of the warmer, wetter Holocene subsequent to the Last Glacial Maximum. The Dwarf Shrubland and Succulent Dwarf Shrubland are depauperate in relation to ccmmunities in other southern African biomes, but the relatively large number of endemics suggests a long history in the region. Their differential species groups occur under arid conditions, accompanied by soils with high base and fertility status. The Dwarf Shrublands may have been more extensive during the drier glacial times on those sites currently occupied by Shrubland. The Shrublands display the expected affInity with the Grassland and Savanna Biomes. The small number of endemics suggest that these communities may have occupied the region in the period since the Last Glacial Maximum. Species with Succulent Karoo Biome affInity are poorly represented. The reliability of using Landsat products to detect and map the vegetation of the region is assessed. The manual classification of Landsat standard products provides a poor reflection of the vegetation of the arid, sparsely-vegetated bottomlands and pediments. The products provide good representation of the boundaries of thicket vegetation, but this uni-temporal approach does not distinguish between floristically different thicket communities. After analyzing digital Landsat data, I suggest that the multi-spectral scanner detects the boundaries of broad soil pedons and geological formations in areas of low vegetative cover. I describe and map the vegetation categories of the region after manual interpretation of six Landsat scenes. This is an effIcient, cost-effective method of mapping vegetation in extensive regions. The mapping units do not reflect the syntaxonomic classification, representing rather an integration of physiographic, pedological, geological and floristic information. With the view to improving the classification of these units, I develop a qualitative model of the natural resources of the region using an expert system
- Full Text:
- Date Issued: 1989
Zinc inhibition of cell division : its relevance to cancer cells and possible mechanism of action
- Authors: Skeef, Noel Samuel
- Date: 1989
- Subjects: Cell division , Cancer cells -- Growth -- Regulation , Zinc in the body , Zinc -- Physiological effect , Cancer -- Research
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4144 , http://hdl.handle.net/10962/d1016266
- Description: A description of two techniques used extensively in this study namely cell counting with a "cell counting plate" and argentation TLC for the separation of ω -6 -fatty acids is given. Zn supplementation into GM of two malignant (BL-6 and Hep- 350) and a non-malignant (LLC-MK) cell line/s resulted in an increased uptake of Zn by the cells and progressively suppressed proliferation of particularly the malignant cells. Zn chelation by EDTA suppressed in vitro proliferation of all 3 cell line, this effect being more pronounced in the malignant cells. A dietary Zn deficiency resulted in alopecia in mice and both a dietary Zn deficiency and Zn excess reduced growth of BL-6 tumours implanted subcutaneously in mice. Zn supplementation into GM progressively increased the uptake of [1-¹⁴C]-LA by BL-6 and LLC-MK cells but had a very slight though irregular effect on this parameter in the Hep- 350 cells. Zn supplementation also stimulated desaturase activity in the BL-6 cells. These results suggested that there are select cell lines whose Δ⁶-desaturase activity responds positively to Zn supplementation (e.g. the BL-6 cells). Delta-6-desaturase activity was also assayed in microsome preparations from different tissues. No enzyme activity was detected in the microsomes prepared from the BL-6 tumours. There was no significant effect with the addition of Zn or EDTA, on Δ⁶-desaturase activity of the regenerating liver microsomes. In the resting liver microsomes this enzyme activity was reduced only when EDTA and Zn were added together and when EDTA was added to the reaction medium as well as to the microsome preparations 2 hr before the enzyme activity assay was initiated. The results of these experiments suggested that the Δ⁶-desaturase enzyme in the microsome preparations may have had an adequate amount of Zn with further additions having no stimulatory effect on the enzyme. Two independent mechanisms of control of cell proliferation by low and high Zn are suggested to operate.
- Full Text:
- Date Issued: 1989
- Authors: Skeef, Noel Samuel
- Date: 1989
- Subjects: Cell division , Cancer cells -- Growth -- Regulation , Zinc in the body , Zinc -- Physiological effect , Cancer -- Research
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4144 , http://hdl.handle.net/10962/d1016266
- Description: A description of two techniques used extensively in this study namely cell counting with a "cell counting plate" and argentation TLC for the separation of ω -6 -fatty acids is given. Zn supplementation into GM of two malignant (BL-6 and Hep- 350) and a non-malignant (LLC-MK) cell line/s resulted in an increased uptake of Zn by the cells and progressively suppressed proliferation of particularly the malignant cells. Zn chelation by EDTA suppressed in vitro proliferation of all 3 cell line, this effect being more pronounced in the malignant cells. A dietary Zn deficiency resulted in alopecia in mice and both a dietary Zn deficiency and Zn excess reduced growth of BL-6 tumours implanted subcutaneously in mice. Zn supplementation into GM progressively increased the uptake of [1-¹⁴C]-LA by BL-6 and LLC-MK cells but had a very slight though irregular effect on this parameter in the Hep- 350 cells. Zn supplementation also stimulated desaturase activity in the BL-6 cells. These results suggested that there are select cell lines whose Δ⁶-desaturase activity responds positively to Zn supplementation (e.g. the BL-6 cells). Delta-6-desaturase activity was also assayed in microsome preparations from different tissues. No enzyme activity was detected in the microsomes prepared from the BL-6 tumours. There was no significant effect with the addition of Zn or EDTA, on Δ⁶-desaturase activity of the regenerating liver microsomes. In the resting liver microsomes this enzyme activity was reduced only when EDTA and Zn were added together and when EDTA was added to the reaction medium as well as to the microsome preparations 2 hr before the enzyme activity assay was initiated. The results of these experiments suggested that the Δ⁶-desaturase enzyme in the microsome preparations may have had an adequate amount of Zn with further additions having no stimulatory effect on the enzyme. Two independent mechanisms of control of cell proliferation by low and high Zn are suggested to operate.
- Full Text:
- Date Issued: 1989
A pastoral theological examination of inner healing
- Authors: Velthuysen, Daniel Nicholas
- Date: 1990
- Subjects: Pastoral theology , Healing -- Religious aspects -- Christianity , Mental healing -- Religious aspects -- Christianity , Inner child -- Religious aspects -- Christianity , Primal therapy -- Religious aspects -- Christianity
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1300 , http://hdl.handle.net/10962/d1016248
- Description: Doing a survey of the ministry of inner healing, one is arrested by three salient features: its pragmatic and correlative development, its lay orientation, and the inconsistent and naïve theoretical explanation of the phenomenon. Inner healing, or as it was first known, the healing of the memories, appears to have its roots with Agnes Sanford during the 1940's (Sandford 1982: 3-4). Over a period of time and through a series of events, Sanford experienced what she termed a healing of memories. After some reflection on her experiences she began to teach her views at the School of Pastoral Care started by her husband in 1958, at Camps Farthest Out (CFO), and at numerous churches and conferences.
- Full Text:
- Date Issued: 1990
- Authors: Velthuysen, Daniel Nicholas
- Date: 1990
- Subjects: Pastoral theology , Healing -- Religious aspects -- Christianity , Mental healing -- Religious aspects -- Christianity , Inner child -- Religious aspects -- Christianity , Primal therapy -- Religious aspects -- Christianity
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1300 , http://hdl.handle.net/10962/d1016248
- Description: Doing a survey of the ministry of inner healing, one is arrested by three salient features: its pragmatic and correlative development, its lay orientation, and the inconsistent and naïve theoretical explanation of the phenomenon. Inner healing, or as it was first known, the healing of the memories, appears to have its roots with Agnes Sanford during the 1940's (Sandford 1982: 3-4). Over a period of time and through a series of events, Sanford experienced what she termed a healing of memories. After some reflection on her experiences she began to teach her views at the School of Pastoral Care started by her husband in 1958, at Camps Farthest Out (CFO), and at numerous churches and conferences.
- Full Text:
- Date Issued: 1990
A study of the reading interests and reading habits of English (first language) secondary-school pupils in South Africa: with particular reference to the Province of Natal
- Authors: Gardner, John Murray
- Date: 1990
- Subjects: Reading interests English language -- Study and teaching (Secondary) -- South Africa -- KwaZulu-Natal Books and reading -- South Africa -- KwaZulu-Natal Readership surveys -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1781 , http://hdl.handle.net/10962/d1003666
- Description: The study derives from a belief, based on many researchers' writings, that wide and frequent book-reading aids the development of knowledge, emotional maturity and human sympathy, which are all essential attributes in a fragmented culture such as South Africa's. It is not accepted that conventional secondary-school literature-teaching in this country promotes a lifelong reading habit among the majority of pupils and a plea is made for the recognition of Reading as a curricular entity in its own right. The study suggests that, owing to a paucity of local research in this field, South African teachers and educational authorities are severely disadvantaged. If they are unable to offer advice based on a proper study of their pupils as readers, they run the risk of guiding many pupils' reading along paths that cannot promise satisfaction and fulfilment. Such stultifying of reading habits would contradict the aims of the present National Core Syllabuses for English (First Language). The thesis sets little store by the investigation of specific bookchoices, pointing out that the validity of such incidental findings, if gleaned from a latitudinal survey, is questionable. Instead, using the findings of questionnaires administered to nearly 2 800 pupils and their teachers, the thesis investigates the relationships between voluntary leisure-time reading and such factors as age, gender, intelligence, academic achievement and standard of living. It also looks at the influences of parents, teachers, peers, contemporary literature-teaching practices, school and public libraries, and leisure- time pursuits other than reading. Many suggestions are offered for further research into finer aspects of those considerations. Many of the findings serve merely to corroborate research from abroad, particularly in respect of age, gender and intelligence. That is none the less alarming when a striking decline in reading is found to occur in the early years of the secondary school. A number of other interesting findings emerge. Standard of living is shown to be inversely correlated with amount of reading, and television-viewing is not found to displace reading. Nor are other leisure-time pursuits found to affect amount of reading: avid readers are by and large extremely active and committed pupils. Reading emerges from the study as providing its own peculiar satisfaction, as does each of the other leisure activities investigated. The challenge is to ensure that infrequent readers become aware of what reading has to offer, and strategies for attempting to achieve that are posited, particularly with regard to the roles of public and school libraries. The roles of teachers and parents are found to be crucial to the development of an appetite for books, and it is suggested that schools and parents liaise formally and closely in this matter. The study groups pupils by a number of personal variables, and investigates their reactions to common forms and genres as well as to certain specifics of style, thereby discussing the relationship between reading, social maturity and academic achievement It argues strongly for recognition of the fact that educating secondary-school pupils in the development of keen and sensitive lifelong reading habits is a process which cannot be systematically taught as a set of skills.
- Full Text:
- Date Issued: 1990
- Authors: Gardner, John Murray
- Date: 1990
- Subjects: Reading interests English language -- Study and teaching (Secondary) -- South Africa -- KwaZulu-Natal Books and reading -- South Africa -- KwaZulu-Natal Readership surveys -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1781 , http://hdl.handle.net/10962/d1003666
- Description: The study derives from a belief, based on many researchers' writings, that wide and frequent book-reading aids the development of knowledge, emotional maturity and human sympathy, which are all essential attributes in a fragmented culture such as South Africa's. It is not accepted that conventional secondary-school literature-teaching in this country promotes a lifelong reading habit among the majority of pupils and a plea is made for the recognition of Reading as a curricular entity in its own right. The study suggests that, owing to a paucity of local research in this field, South African teachers and educational authorities are severely disadvantaged. If they are unable to offer advice based on a proper study of their pupils as readers, they run the risk of guiding many pupils' reading along paths that cannot promise satisfaction and fulfilment. Such stultifying of reading habits would contradict the aims of the present National Core Syllabuses for English (First Language). The thesis sets little store by the investigation of specific bookchoices, pointing out that the validity of such incidental findings, if gleaned from a latitudinal survey, is questionable. Instead, using the findings of questionnaires administered to nearly 2 800 pupils and their teachers, the thesis investigates the relationships between voluntary leisure-time reading and such factors as age, gender, intelligence, academic achievement and standard of living. It also looks at the influences of parents, teachers, peers, contemporary literature-teaching practices, school and public libraries, and leisure- time pursuits other than reading. Many suggestions are offered for further research into finer aspects of those considerations. Many of the findings serve merely to corroborate research from abroad, particularly in respect of age, gender and intelligence. That is none the less alarming when a striking decline in reading is found to occur in the early years of the secondary school. A number of other interesting findings emerge. Standard of living is shown to be inversely correlated with amount of reading, and television-viewing is not found to displace reading. Nor are other leisure-time pursuits found to affect amount of reading: avid readers are by and large extremely active and committed pupils. Reading emerges from the study as providing its own peculiar satisfaction, as does each of the other leisure activities investigated. The challenge is to ensure that infrequent readers become aware of what reading has to offer, and strategies for attempting to achieve that are posited, particularly with regard to the roles of public and school libraries. The roles of teachers and parents are found to be crucial to the development of an appetite for books, and it is suggested that schools and parents liaise formally and closely in this matter. The study groups pupils by a number of personal variables, and investigates their reactions to common forms and genres as well as to certain specifics of style, thereby discussing the relationship between reading, social maturity and academic achievement It argues strongly for recognition of the fact that educating secondary-school pupils in the development of keen and sensitive lifelong reading habits is a process which cannot be systematically taught as a set of skills.
- Full Text:
- Date Issued: 1990
Alternative mythical structures in the fiction of Patrick White
- Authors: Bosman, Brenda Evadne
- Date: 1990
- Subjects: White, Patrick, 1912-1990 , White, Patrick, 1912-1990 -- Criticism and interpretation , Myth in literature
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2170 , http://hdl.handle.net/10962/d1001821
- Description: The texts in this study interrogate the dominant myths which have affected the constructs of identity and history in the white Australian socio-historical context. These myths are exposed by White as ideologically determined and as operating by processes of exclusion, repression and marginalisation. White challenges the autonomy of both European and Australian cultures, reveals the ideological complicity between them and adopts a critical approach to all Western cultural assumptions. As a post-colonial writer, White shares the need of both post-colonising and post-colonised groups for an identity established not in terms of the colonial power but in terms of themselves. As a dissident white male, he is a privileged member of the post- colonising group but one who rejects the dominant discourses as illegitimate and unlegitimating. He offers a re-writing of the myths underpinning colonial and post-colonising discourses which privileges their suppressed and repressed elements. His re-writings affect aboriginal men and women, white women and the 'privileged' white male whose subjection to social control is masked as unproblematic freedom. White's re-writing of myth enbraces the post-modern as well as the post- colonial. He not only deconstructs and demystifies the phallogocentric/ethnocentric order of things; he also attempts to avoid totalization by privileging indeterminacy, fragmentation, hybridization and those liminary states which defy articulation: the ecstatic, the abject, the unspeakable. He himself is denied authority in that his re-writings are presented as mere acts in the always provisional process of making interpretations. White acknowledges the problematics of both presentation and re-presentation - an unresolved tension between the post-colonial desire for self-definition and the post-modern decentring of all meaning and interpretation permeates his discourse. The close readings of the texts attempt, accordingly, to reflect varying oppositional strategies: those which seek to overturn hierarchies and expose power-relations and those which seek an idiom in which contemporary Australia may find its least distorted reflexion. Within this ideological context, the Lacanian thematics of the subject, and their re-writing by Kristeva, are linked with dialectical criticism in an attempt to reflect a strictly provisional process of (re) construction
- Full Text:
- Date Issued: 1990
- Authors: Bosman, Brenda Evadne
- Date: 1990
- Subjects: White, Patrick, 1912-1990 , White, Patrick, 1912-1990 -- Criticism and interpretation , Myth in literature
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2170 , http://hdl.handle.net/10962/d1001821
- Description: The texts in this study interrogate the dominant myths which have affected the constructs of identity and history in the white Australian socio-historical context. These myths are exposed by White as ideologically determined and as operating by processes of exclusion, repression and marginalisation. White challenges the autonomy of both European and Australian cultures, reveals the ideological complicity between them and adopts a critical approach to all Western cultural assumptions. As a post-colonial writer, White shares the need of both post-colonising and post-colonised groups for an identity established not in terms of the colonial power but in terms of themselves. As a dissident white male, he is a privileged member of the post- colonising group but one who rejects the dominant discourses as illegitimate and unlegitimating. He offers a re-writing of the myths underpinning colonial and post-colonising discourses which privileges their suppressed and repressed elements. His re-writings affect aboriginal men and women, white women and the 'privileged' white male whose subjection to social control is masked as unproblematic freedom. White's re-writing of myth enbraces the post-modern as well as the post- colonial. He not only deconstructs and demystifies the phallogocentric/ethnocentric order of things; he also attempts to avoid totalization by privileging indeterminacy, fragmentation, hybridization and those liminary states which defy articulation: the ecstatic, the abject, the unspeakable. He himself is denied authority in that his re-writings are presented as mere acts in the always provisional process of making interpretations. White acknowledges the problematics of both presentation and re-presentation - an unresolved tension between the post-colonial desire for self-definition and the post-modern decentring of all meaning and interpretation permeates his discourse. The close readings of the texts attempt, accordingly, to reflect varying oppositional strategies: those which seek to overturn hierarchies and expose power-relations and those which seek an idiom in which contemporary Australia may find its least distorted reflexion. Within this ideological context, the Lacanian thematics of the subject, and their re-writing by Kristeva, are linked with dialectical criticism in an attempt to reflect a strictly provisional process of (re) construction
- Full Text:
- Date Issued: 1990
Aspects of the biology of the infaunal bivalve Mollusc Solen cylindraceus (Hanley) in the Kariega estuary
- De Villiers, Casper Johannes
- Authors: De Villiers, Casper Johannes
- Date: 1990
- Subjects: Mollusks -- South Africa -- Eastern Cape Bivalves -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5639 , http://hdl.handle.net/10962/d1005321
- Description: Solen cylindraceus is an infaunal filter-feeding bivalve inhabiting the intertidal mud banks of many southern African estuaries. It is particularly abundant in the Kariega estuary (33°41'S; 26°42'E) where it reaches densities of 400m⁻² (192g shell-free dry wt. m⁻²). The Kariega is a permanently open, marine dominated estuary about 18km in length, and S. cylindraceus is most abundant in its middle and upper reaches. Some physical characteristics of the estuary (temperature, salinity, sediment and water turbidity) are described, and the possible role of these factors in determining the density and distribution of S. cylindraceus within the Kariega estuary, is discussed. The structure of the alimentary system, gills and labial palps of S. cylintfraceus is described, all of which showed no major variation from the "typical" eulamellibranchiate form. Solen cylintfraceus was found to be a euryhaline osmoconformer with a salinity tolerance range of 15-65%. When animals were removed from their burrows, osmotic equilibration of the haemolymph was rapid (1-2 hours). By contrast, in animals left undisturbed in their burrows, osmotic equilibration was retarded (72-204 hours). It is suggested that the observed decrease in the rate of change of haemolymph osmolarity for animals in their burrows is linked to the stability of the interstitial salinity. A temperature tolerance range of 5-44°C was determined for S. cylintfraceus (in situ), in which prolonged exposure to 5°C and 40-45°C (12-36 hours respectively) resulted in a decreased burrowing ability, coma and death. Animal burrowing responses were not affected by temperatures in the range 15-35°C. Field experiments were carried out over several tidal cycles, in which the measurement of crystalline style volume was used as a means of assessing extracellular digestive activity. No major variation in style volume was recorded and it appeared that S. cylindraceus did not exhibit any cyclical pattern of style dissolution and regeneration. It is suggested that S. cylindraceus feeds continuously from the water column during high tide and possibly within its burrow, at or below the water table, during low tide. At a suspensoid concentration of 5Omg l⁻¹, S. cylindraceus was found to filter water almost continuously (90-95% of the time). Time spent filtering dropped to 68% at 100mg l⁻¹ and 32% at 500mg l⁻¹. Filtration rates for summer collected animals (25°C) were 22.86 ± 4.36ml min.⁻¹, some 3ml min.⁻¹ greater than that recorded for winter (16°C) collected animals. Filtration rate may be expressed as a function of shell length by the equations: y=0.247x¹̇⁰⁶⁶ (winter) and y=0.758x⁰̇⁸²⁶ (summer). Solen cylindraceus was capable of acclimating its filtration rate to both high and low temperatures under laboratory conditions. Filtration rate exhibited a thermal optimum in the range 15-35°C, declining at higher and lower temperatures. Q₁₀ values of filtration decreased rapidly from greater than 4 to less than 2, when the thermal optimum was reached. Maximum rates generally occurred at approximately 5°C above the temperature to which the animal had been acclimated. Optimal filtration rates (19-23ml min.⁻¹) were recorded in the salinity range 15-45%. When subjected to abrupt changes in salinity, filtration rates were immediately depressed. The extent and duration of these decreased filtration rates were dependent upon the magnitude and direction of salinity change, and were always less in animals exposed to hyper- than hyposaline conditions. Animals exposed to increased temperature and simultaneous elevated or unchanged salinity, showed a slight increase in filtration rate followed by rapid acclimation. A decrease in both temperature and salinity resulted in an initial decrease in filtration rate and a longer acclimation period. The ability of S. cylindraceus to acclimate fully within a wide temperature and salinity range, and to filter maximally in hypersaline conditions may, in part, explain its unusually high abundance in the Kariega estuary, despite it being close to the southernmost limit of the animal's geographical distribution. No significant difference in flItration rate was recorded at suspensoid concentrations of 5-100mg 1⁻¹. However, at 250 and 500mg l⁻¹ filtration rates decreased significantly, and coincided with increased levels of pseudofaecal production. Solen cylindraceus retained particles down to 2.5-3.0µm with great efficiency (ca. 60-90% efficiency). Below this particle size, retention efficiency decreased rapidly and a net production of particles was recorded below 1.51µm. Particle retention was independent of temperature (15 and 25°C) and salinity (15 and 35%). Use was made of stable carbon isotope analyses (¹³C/¹²C ratios) in an attempt to determine the important food sources of S. cylindraceus within the Kariega estuary. The results obtained demonstrated an enrichment in δ¹³ values for S. cylindraceus from the upper (-27.9%) to the middle (-25%c) and lower (-21.6%o ) reaches of the estuary, with no seasonal variation apparent. The bivalve was substantially more depleted in ¹³C relative to the dominant aquatic macrophytes Zostera capensis (-9.1 to -15.6%o) and Spartina maritima (-12.5%o). The use of δ¹³ alone, however, to unequivocally "pin point" specific food sources of a filter feeder in a predominantly detritus based food web, is limited. It is suggested that in the Kariega estuary, riparian litter and other terrestrially derived vegetation contribute to the carbon pool. A possible contribution of ¹³C depleted food sources via chemoautotrophic and/or anaerobic pathways, to the diet of S. cylindraceus, is suggested.
- Full Text:
- Date Issued: 1990
- Authors: De Villiers, Casper Johannes
- Date: 1990
- Subjects: Mollusks -- South Africa -- Eastern Cape Bivalves -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5639 , http://hdl.handle.net/10962/d1005321
- Description: Solen cylindraceus is an infaunal filter-feeding bivalve inhabiting the intertidal mud banks of many southern African estuaries. It is particularly abundant in the Kariega estuary (33°41'S; 26°42'E) where it reaches densities of 400m⁻² (192g shell-free dry wt. m⁻²). The Kariega is a permanently open, marine dominated estuary about 18km in length, and S. cylindraceus is most abundant in its middle and upper reaches. Some physical characteristics of the estuary (temperature, salinity, sediment and water turbidity) are described, and the possible role of these factors in determining the density and distribution of S. cylindraceus within the Kariega estuary, is discussed. The structure of the alimentary system, gills and labial palps of S. cylintfraceus is described, all of which showed no major variation from the "typical" eulamellibranchiate form. Solen cylintfraceus was found to be a euryhaline osmoconformer with a salinity tolerance range of 15-65%. When animals were removed from their burrows, osmotic equilibration of the haemolymph was rapid (1-2 hours). By contrast, in animals left undisturbed in their burrows, osmotic equilibration was retarded (72-204 hours). It is suggested that the observed decrease in the rate of change of haemolymph osmolarity for animals in their burrows is linked to the stability of the interstitial salinity. A temperature tolerance range of 5-44°C was determined for S. cylintfraceus (in situ), in which prolonged exposure to 5°C and 40-45°C (12-36 hours respectively) resulted in a decreased burrowing ability, coma and death. Animal burrowing responses were not affected by temperatures in the range 15-35°C. Field experiments were carried out over several tidal cycles, in which the measurement of crystalline style volume was used as a means of assessing extracellular digestive activity. No major variation in style volume was recorded and it appeared that S. cylindraceus did not exhibit any cyclical pattern of style dissolution and regeneration. It is suggested that S. cylindraceus feeds continuously from the water column during high tide and possibly within its burrow, at or below the water table, during low tide. At a suspensoid concentration of 5Omg l⁻¹, S. cylindraceus was found to filter water almost continuously (90-95% of the time). Time spent filtering dropped to 68% at 100mg l⁻¹ and 32% at 500mg l⁻¹. Filtration rates for summer collected animals (25°C) were 22.86 ± 4.36ml min.⁻¹, some 3ml min.⁻¹ greater than that recorded for winter (16°C) collected animals. Filtration rate may be expressed as a function of shell length by the equations: y=0.247x¹̇⁰⁶⁶ (winter) and y=0.758x⁰̇⁸²⁶ (summer). Solen cylindraceus was capable of acclimating its filtration rate to both high and low temperatures under laboratory conditions. Filtration rate exhibited a thermal optimum in the range 15-35°C, declining at higher and lower temperatures. Q₁₀ values of filtration decreased rapidly from greater than 4 to less than 2, when the thermal optimum was reached. Maximum rates generally occurred at approximately 5°C above the temperature to which the animal had been acclimated. Optimal filtration rates (19-23ml min.⁻¹) were recorded in the salinity range 15-45%. When subjected to abrupt changes in salinity, filtration rates were immediately depressed. The extent and duration of these decreased filtration rates were dependent upon the magnitude and direction of salinity change, and were always less in animals exposed to hyper- than hyposaline conditions. Animals exposed to increased temperature and simultaneous elevated or unchanged salinity, showed a slight increase in filtration rate followed by rapid acclimation. A decrease in both temperature and salinity resulted in an initial decrease in filtration rate and a longer acclimation period. The ability of S. cylindraceus to acclimate fully within a wide temperature and salinity range, and to filter maximally in hypersaline conditions may, in part, explain its unusually high abundance in the Kariega estuary, despite it being close to the southernmost limit of the animal's geographical distribution. No significant difference in flItration rate was recorded at suspensoid concentrations of 5-100mg 1⁻¹. However, at 250 and 500mg l⁻¹ filtration rates decreased significantly, and coincided with increased levels of pseudofaecal production. Solen cylindraceus retained particles down to 2.5-3.0µm with great efficiency (ca. 60-90% efficiency). Below this particle size, retention efficiency decreased rapidly and a net production of particles was recorded below 1.51µm. Particle retention was independent of temperature (15 and 25°C) and salinity (15 and 35%). Use was made of stable carbon isotope analyses (¹³C/¹²C ratios) in an attempt to determine the important food sources of S. cylindraceus within the Kariega estuary. The results obtained demonstrated an enrichment in δ¹³ values for S. cylindraceus from the upper (-27.9%) to the middle (-25%c) and lower (-21.6%o ) reaches of the estuary, with no seasonal variation apparent. The bivalve was substantially more depleted in ¹³C relative to the dominant aquatic macrophytes Zostera capensis (-9.1 to -15.6%o) and Spartina maritima (-12.5%o). The use of δ¹³ alone, however, to unequivocally "pin point" specific food sources of a filter feeder in a predominantly detritus based food web, is limited. It is suggested that in the Kariega estuary, riparian litter and other terrestrially derived vegetation contribute to the carbon pool. A possible contribution of ¹³C depleted food sources via chemoautotrophic and/or anaerobic pathways, to the diet of S. cylindraceus, is suggested.
- Full Text:
- Date Issued: 1990
Aspects of the nutritional physiology and dietary requirements of juvenile and adult sharptooth catfish, Clarias Gariepinus (Pisces : clariidae)
- Authors: Uys, Wynand
- Date: 1990
- Subjects: Clariidae Catfishes Catfishes -- Nutrition-requirements Clarias
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5225 , http://hdl.handle.net/10962/d1005068
- Description: Past and current research on the biology and culture of the sharptooth catfish (Clarias gariepinus) has stimulated the development of its commercial production. At the inception of this project in 1985, it was decided that the development of an optimal dry feed was the most important step in developing a more complete culture technology for the species. The objectives of the project were, therefore, to investigate aspects of the nutritional physiology and dietary requirements of the species to provide the necessary information for the formulation of optimum economical feeds. A review of the natural feeding biology of the species provided valuable information with regard to its food preferences. From this information, and by estimating the nutritional composition of its preferred natural diet, it could be concluded that the animal requires a relatively high dietary protein content. This conclusion was substantiated by studies of the functional morphology of the digestive system (gross and fine structure). The characteristics of the digestive system and external anatomy were found to be typical of an opportunistic, omnivorous predator. The ontogenetic development of the digestive system is relatively fast, and enables the utilization of a variety of diets efficiently from an early stage. Since the ability of an animal to digest a given substance is predominantly dependent on the presence of appropriate enzymes the quantification and characterization of C. gariepinus digestive enzyme activities were investigated on an experimental basis. This work revealed that the animal has relatively high levels of digestive enzyme activities, and that its proteolytic enzyme activities correspond with those of other carnivorous fishes, while its starch digesting abilities correspond with those of specialized herbivores. Also, the high levels of lysozyme and alkaline phosphatase indicate an adaptation to detritivory, making this fish a truly opportunistic omnivore. Evidence was found to suggest that sharptooth catfish are able to re-absorb secreted digestive enzymes in the posterior section of its intestinal tract. It was shown that digestive enzyme activities were induced by food intake, and that no inherent rhythmicity in digestive activities seemed to occur. The development of digestive enzyme systems in the early life history of this animal was found to be also extremely rapid (complete within 10-16 days after hatching). It was also shown that artificial dry feed stimulates higher levels of digestive enzyme activities in larvae and juveniles than natural food. Although increased cellulase activity was found in larvae and juveniles that were fed on live zooplankton, the relative contribution of exogenous prey enzymes to protein digestion could not be ascertained. Specific nutritional requirements were investigated by means of feeding trials. The results of these trials confirmed the above indications regarding the dietary requirements of sharptooth catfish. It was concluded that for optimal growth and production performance, this animal requires a dietary protein content of 40-42%, a dietary lipid content of 10-12% and that diets should contain 14-16 kJ/g digestible energy. The optimum protein-to-energy ratio was found to be 26-29mg protein per kJ of digestible energy. Dietary requirements are evidently the same for juveniles and adults, except that younger fish require higher relative feeding levels. Finally, practical feed formulations were evaluated in terms of their economic feasibility. The results of these trials are presented along with recommendations on the formulation of commercial feeds for C. gariepinus. A computer program with which to implement least cost feed formulation by means of linear programming is provided.
- Full Text:
- Date Issued: 1990
- Authors: Uys, Wynand
- Date: 1990
- Subjects: Clariidae Catfishes Catfishes -- Nutrition-requirements Clarias
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5225 , http://hdl.handle.net/10962/d1005068
- Description: Past and current research on the biology and culture of the sharptooth catfish (Clarias gariepinus) has stimulated the development of its commercial production. At the inception of this project in 1985, it was decided that the development of an optimal dry feed was the most important step in developing a more complete culture technology for the species. The objectives of the project were, therefore, to investigate aspects of the nutritional physiology and dietary requirements of the species to provide the necessary information for the formulation of optimum economical feeds. A review of the natural feeding biology of the species provided valuable information with regard to its food preferences. From this information, and by estimating the nutritional composition of its preferred natural diet, it could be concluded that the animal requires a relatively high dietary protein content. This conclusion was substantiated by studies of the functional morphology of the digestive system (gross and fine structure). The characteristics of the digestive system and external anatomy were found to be typical of an opportunistic, omnivorous predator. The ontogenetic development of the digestive system is relatively fast, and enables the utilization of a variety of diets efficiently from an early stage. Since the ability of an animal to digest a given substance is predominantly dependent on the presence of appropriate enzymes the quantification and characterization of C. gariepinus digestive enzyme activities were investigated on an experimental basis. This work revealed that the animal has relatively high levels of digestive enzyme activities, and that its proteolytic enzyme activities correspond with those of other carnivorous fishes, while its starch digesting abilities correspond with those of specialized herbivores. Also, the high levels of lysozyme and alkaline phosphatase indicate an adaptation to detritivory, making this fish a truly opportunistic omnivore. Evidence was found to suggest that sharptooth catfish are able to re-absorb secreted digestive enzymes in the posterior section of its intestinal tract. It was shown that digestive enzyme activities were induced by food intake, and that no inherent rhythmicity in digestive activities seemed to occur. The development of digestive enzyme systems in the early life history of this animal was found to be also extremely rapid (complete within 10-16 days after hatching). It was also shown that artificial dry feed stimulates higher levels of digestive enzyme activities in larvae and juveniles than natural food. Although increased cellulase activity was found in larvae and juveniles that were fed on live zooplankton, the relative contribution of exogenous prey enzymes to protein digestion could not be ascertained. Specific nutritional requirements were investigated by means of feeding trials. The results of these trials confirmed the above indications regarding the dietary requirements of sharptooth catfish. It was concluded that for optimal growth and production performance, this animal requires a dietary protein content of 40-42%, a dietary lipid content of 10-12% and that diets should contain 14-16 kJ/g digestible energy. The optimum protein-to-energy ratio was found to be 26-29mg protein per kJ of digestible energy. Dietary requirements are evidently the same for juveniles and adults, except that younger fish require higher relative feeding levels. Finally, practical feed formulations were evaluated in terms of their economic feasibility. The results of these trials are presented along with recommendations on the formulation of commercial feeds for C. gariepinus. A computer program with which to implement least cost feed formulation by means of linear programming is provided.
- Full Text:
- Date Issued: 1990
Between freedom and givenness: (a study of the hermeneutical consequences of the concept of canon for the authority of scripture)
- Authors: Latham, Jonathan Cyril
- Date: 1990
- Subjects: Bible -- Evidences, authority, etc. , Authority -- Religious aspects , Canon (Literature
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1216 , http://hdl.handle.net/10962/d1001546
- Description: The aim of this thesis is to arrive at an understanding of the authority of scripture that is able to accommodate both a faith perspective and the fruits of the historical-critical approach to the New Testament. Put differently, the aim of this thesis is the pursuit of a specifically christian, faith-promoting, reading of the New Testament whilst still enjoying the benefit, in an as uncompromised a form as possible, of the historical- critical approach. In a sense it may be said that this task, given that the roots of both the historical-critical approach and modern Western culture are deeply imbedded in Rationalism, is equivalent to the basic hermeneutical question of whether it is possible to interpret scripture relevantly from within a cultural web of meaning that does not readily accommodate that embodied in the New Testament. In section one of this dissertation we present a characteristic depiction, based on the historical-critical theory of literature, of the authority of the New Testament. This is followed by a brief assessment that makes explicit why the historical- critical approach is not conducive to the adoption of a faith perspective on these writings. In section two, still and inevitably based on critical foundations, we adopt a perspective that is more sympathetic to faith and that seeks to discover in the concerns evidenced in the canonical process, when traditions about Jesus gradually took on more complex and stable forms, culminating in the canon of the New Testament, guidelines in helping us to deal with the problem with which this study is concerned . In the specific example of the rather ordinary concerns underlying the unusual history of the pericope de adultera (John 7:53-8:11), examined against the background of the interests underlying the canonical process, it becomes clear that christians from the very beginning faced a dilemma not unlike that with which the historical-critical approach confronts us. They had to interpret afresh, and faithfully, the traditions in order to meet the demands of situations that had never been foreseen by earlier tradents. In this respect, therefore, the history of the pericope de adultera presents us with an ongoing struggle to hold in tension the demands of new contexts with the imperative of strict continuity with Jesus. In section three, on the basis of the foundation of the authority of scripture in strict continuity with Jesus combined with the contextual reinterpretation of the tradition, the social sciences are employed. Using the social sciences, it is discovered that the two contradictory approaches that we wish to reconcile form part of two different models for interpreting reality. It is on this basis, and made possible by the common culture underlying these opposing models and by the common contact with an unspecified common core of concrete reality, that a solution is proposed in terms of a complex 'fusion of horizons', promoted by a 'precipitative environment'. In the conclusion our solution is decisively aligned with the concerns evidenced in the canonical process
- Full Text:
- Date Issued: 1990
- Authors: Latham, Jonathan Cyril
- Date: 1990
- Subjects: Bible -- Evidences, authority, etc. , Authority -- Religious aspects , Canon (Literature
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1216 , http://hdl.handle.net/10962/d1001546
- Description: The aim of this thesis is to arrive at an understanding of the authority of scripture that is able to accommodate both a faith perspective and the fruits of the historical-critical approach to the New Testament. Put differently, the aim of this thesis is the pursuit of a specifically christian, faith-promoting, reading of the New Testament whilst still enjoying the benefit, in an as uncompromised a form as possible, of the historical- critical approach. In a sense it may be said that this task, given that the roots of both the historical-critical approach and modern Western culture are deeply imbedded in Rationalism, is equivalent to the basic hermeneutical question of whether it is possible to interpret scripture relevantly from within a cultural web of meaning that does not readily accommodate that embodied in the New Testament. In section one of this dissertation we present a characteristic depiction, based on the historical-critical theory of literature, of the authority of the New Testament. This is followed by a brief assessment that makes explicit why the historical- critical approach is not conducive to the adoption of a faith perspective on these writings. In section two, still and inevitably based on critical foundations, we adopt a perspective that is more sympathetic to faith and that seeks to discover in the concerns evidenced in the canonical process, when traditions about Jesus gradually took on more complex and stable forms, culminating in the canon of the New Testament, guidelines in helping us to deal with the problem with which this study is concerned . In the specific example of the rather ordinary concerns underlying the unusual history of the pericope de adultera (John 7:53-8:11), examined against the background of the interests underlying the canonical process, it becomes clear that christians from the very beginning faced a dilemma not unlike that with which the historical-critical approach confronts us. They had to interpret afresh, and faithfully, the traditions in order to meet the demands of situations that had never been foreseen by earlier tradents. In this respect, therefore, the history of the pericope de adultera presents us with an ongoing struggle to hold in tension the demands of new contexts with the imperative of strict continuity with Jesus. In section three, on the basis of the foundation of the authority of scripture in strict continuity with Jesus combined with the contextual reinterpretation of the tradition, the social sciences are employed. Using the social sciences, it is discovered that the two contradictory approaches that we wish to reconcile form part of two different models for interpreting reality. It is on this basis, and made possible by the common culture underlying these opposing models and by the common contact with an unspecified common core of concrete reality, that a solution is proposed in terms of a complex 'fusion of horizons', promoted by a 'precipitative environment'. In the conclusion our solution is decisively aligned with the concerns evidenced in the canonical process
- Full Text:
- Date Issued: 1990
Chemical and spectroscopic studies of the capsular polysaccharides of some klebsiella and escherichia coli serotypes
- Authors: Stanley, Shawn Mark Ross
- Date: 1990
- Subjects: Polysaccharides , Klebsiella , Escherichia
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3736 , http://hdl.handle.net/10962/d1001525
- Description: The work described in this thesis forms part of an international programme concerned with the structure elucidation of the capsular antigens of some Enterobacteriaceae. Many of the Klebsiella and some of the Escherichia coli are pathogenic to man and, hence, they are of interest. The virulence of bacteria is a multifactorial phenomenon, in which characteristic traits of bacteria and their hosts play comparable and complementary roles. It is accepted that pathogens are more virulent when encapsulated, because, nearly all disease causing bacteria have a capsule when freshly isolated from the host. This increase in pathogenicity is related, in part, to the capsular polysaccharides' ability to avoid or attenuate the host defence mechanisms. In the majority of cases the protective aspects of the capsule are overcome in the latter stages of infection when the formation of specific antibodies by the host has occurred. However there are situations in which an immune state of the infected host is virtually never reached, and susceptiblity to the infecting bacteria is maintained even in the advanced stage of an infection. Explanation of this phenomenon becomes possible by analysing the structure of the polysaccharides. The inability of the host to raise an immune response to the capsule may be because the structure of the polysaccharide is similar or identical to the host's carbohydrates. The serological and pathogenic relatedness of encapsulated E. coli and Klebsiella, to the encapsulated strains of other genera, is based on structural identity or similarity of the respective capsules. Capsular polysaccharides are analysed by both chemical and instrumental methods, and, at present, nuclear magnetic resonance spectroscopy is the most important analytical technique
- Full Text:
- Date Issued: 1990
- Authors: Stanley, Shawn Mark Ross
- Date: 1990
- Subjects: Polysaccharides , Klebsiella , Escherichia
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3736 , http://hdl.handle.net/10962/d1001525
- Description: The work described in this thesis forms part of an international programme concerned with the structure elucidation of the capsular antigens of some Enterobacteriaceae. Many of the Klebsiella and some of the Escherichia coli are pathogenic to man and, hence, they are of interest. The virulence of bacteria is a multifactorial phenomenon, in which characteristic traits of bacteria and their hosts play comparable and complementary roles. It is accepted that pathogens are more virulent when encapsulated, because, nearly all disease causing bacteria have a capsule when freshly isolated from the host. This increase in pathogenicity is related, in part, to the capsular polysaccharides' ability to avoid or attenuate the host defence mechanisms. In the majority of cases the protective aspects of the capsule are overcome in the latter stages of infection when the formation of specific antibodies by the host has occurred. However there are situations in which an immune state of the infected host is virtually never reached, and susceptiblity to the infecting bacteria is maintained even in the advanced stage of an infection. Explanation of this phenomenon becomes possible by analysing the structure of the polysaccharides. The inability of the host to raise an immune response to the capsule may be because the structure of the polysaccharide is similar or identical to the host's carbohydrates. The serological and pathogenic relatedness of encapsulated E. coli and Klebsiella, to the encapsulated strains of other genera, is based on structural identity or similarity of the respective capsules. Capsular polysaccharides are analysed by both chemical and instrumental methods, and, at present, nuclear magnetic resonance spectroscopy is the most important analytical technique
- Full Text:
- Date Issued: 1990
Essential fatty acids and ascorbic acid- interactions and effects on melanoma growth
- Authors: Gardiner, Neil Stockenstrom
- Date: 1990
- Subjects: Fatty acids , Melanoma , Mice -- Diseases
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4549 , http://hdl.handle.net/10962/d1018230
- Description: The present study was carried out to determine the effects and possible mechanisms of action of the essential fatty acids (EFAs) (linoleic acid (LA), gamma-linolenic acid (GLA) and arachidonic acid (AA)) and ascorbic acid (Asc) on BL6 murine melanoma growth in cell culture and in mice. Interactions between the nutrients in influencing melanoma growth as well as possible mechanisms of the interactions were also examined in the above systems. Cell culture studies revealed that all three EFAs (0-SOμg/ml) and Asc (0-200μg/ml) significantly inhibited melanoma growth at the concentrations used. The EF As were also found to significantly inhibit growth, although to a lesser extent than BL6 cells, of monkey kidney (LLCMK) cells which were used as a non-malignant control cell line. Asc in contrast was found not to inhibit growth of these cells. Supplementation of Asc (lOO)μg/ml) to EFA containing (0-50μg/ml) medium was found to significantly increase inhibition of cell growth in both cell lines, and in the BL6 cells in particular, after taking into account the growth inhibitory effects of Asc in the absence of EFAs. The mechanism of cell growth inhibition by the EF As appeared to involve lipid peroxidation but not enhanced prostaglandin (PG) or leukotriene (LT) synthesis. While Asc was found to increase both lipid peroxidation and PG synthesis in the cells, these mechanisms and enhanced LT synthesis did not appear to have played a role in the inhibition of cell growth by Asc or in the growth inhibitory interaction between Asc and the EF As. In vivo studies revealed that diets containing essential or polyunsaturated fatty acids (EFAs/PUFAs) in the form of vegetable oils, and in particular GLA in the form of evening primrose oil, significantly promoted melanoma growth in mice when compared with an EFA/PUFA free diet containing predominantly saturated fats (SF). Supplementary dietary Asc in contrast was found to significantly inhibit melanoma growth in mice fed EFA/PUFA, and in particular GLA, containing diets but not in mice fed SF cont~g diets. This result appears to indicate the occurrence of an interaction between the two nutrients. Ul The mechanism of tumour promotion by the EP As/PUP As did not appear to have involved enhanced PG or LT synthesis or lipid peroxidation. Since dietary EPA/PUPA manipulation was found to significantly alter the EPA content of tissues, including the melanomas, the mechanism of tumour promotion may have involved changes in the EPA composition of the tumour cells. While supplementary Asc was found to significantly increase the Asc content of certain tissues, including the melanomas, which may have played a role in tumour growth inhibition by Asc, it was found not to affect the EPA content of tissues. Enhanced PG or LT synthesis and lipid perox:idation did not appear to have been involved in the tumour growth inhibitory interaction between Asc and the EP As/PUP As. THe activity of the enzyme delta-6-desaturase, a key enzyme in EF A metabolism which catalyses the desaturation of LA to GLA, and the influence of Asc on activity of the enzyme were also examined. The cultured cells, and BL6 cells in particular, were found to contain significant activity of the enzyme. Whereas murine liver microsomal fractions were found to contain delta-6-desaturase activity, microsomes from melanomas grown in mice were found to lack activity of the enzyme. The significant tumour promoting effects of the GLA containing EPO diet may have been the result of the lack of delta-6-desaturase activity in tumour cells grown in mice. Asc was found to stimulate activity of the enzyme in cultured BL6 cells but not in LLCM.K cells, while dietary Asc and EF A/PUP A manipulation did not influence activity of the enzyme in microsomal fractions. This study has confirmed previous reports of the in vivo tumour promoting effects of dietary EP As/PUP As and the tumour growth inhibitory effects of Asc. The in vitro cell growth inhibitory effects of Asc and the EP As also confirm the results of previous reports. Previous studies investigating possible interactions between Asc and EP As/PUP As in influencing tumour cell growth could not be located in the relevant literature. This study may therefore be one of the first investigations of any such interaction between these nutrients in tumour cells. While this study was not able to identify the mechanisms involved in the different tumour promoting or tumour growth inhibitory effects of the two nutrients in the two systems, it did eliminate a number of potential mechanisms. The results of this study also emphasise the difficulty of attempting to compare the results of in vitro and in vivo studies.
- Full Text:
- Date Issued: 1990
- Authors: Gardiner, Neil Stockenstrom
- Date: 1990
- Subjects: Fatty acids , Melanoma , Mice -- Diseases
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4549 , http://hdl.handle.net/10962/d1018230
- Description: The present study was carried out to determine the effects and possible mechanisms of action of the essential fatty acids (EFAs) (linoleic acid (LA), gamma-linolenic acid (GLA) and arachidonic acid (AA)) and ascorbic acid (Asc) on BL6 murine melanoma growth in cell culture and in mice. Interactions between the nutrients in influencing melanoma growth as well as possible mechanisms of the interactions were also examined in the above systems. Cell culture studies revealed that all three EFAs (0-SOμg/ml) and Asc (0-200μg/ml) significantly inhibited melanoma growth at the concentrations used. The EF As were also found to significantly inhibit growth, although to a lesser extent than BL6 cells, of monkey kidney (LLCMK) cells which were used as a non-malignant control cell line. Asc in contrast was found not to inhibit growth of these cells. Supplementation of Asc (lOO)μg/ml) to EFA containing (0-50μg/ml) medium was found to significantly increase inhibition of cell growth in both cell lines, and in the BL6 cells in particular, after taking into account the growth inhibitory effects of Asc in the absence of EFAs. The mechanism of cell growth inhibition by the EF As appeared to involve lipid peroxidation but not enhanced prostaglandin (PG) or leukotriene (LT) synthesis. While Asc was found to increase both lipid peroxidation and PG synthesis in the cells, these mechanisms and enhanced LT synthesis did not appear to have played a role in the inhibition of cell growth by Asc or in the growth inhibitory interaction between Asc and the EF As. In vivo studies revealed that diets containing essential or polyunsaturated fatty acids (EFAs/PUFAs) in the form of vegetable oils, and in particular GLA in the form of evening primrose oil, significantly promoted melanoma growth in mice when compared with an EFA/PUFA free diet containing predominantly saturated fats (SF). Supplementary dietary Asc in contrast was found to significantly inhibit melanoma growth in mice fed EFA/PUFA, and in particular GLA, containing diets but not in mice fed SF cont~g diets. This result appears to indicate the occurrence of an interaction between the two nutrients. Ul The mechanism of tumour promotion by the EP As/PUP As did not appear to have involved enhanced PG or LT synthesis or lipid peroxidation. Since dietary EPA/PUPA manipulation was found to significantly alter the EPA content of tissues, including the melanomas, the mechanism of tumour promotion may have involved changes in the EPA composition of the tumour cells. While supplementary Asc was found to significantly increase the Asc content of certain tissues, including the melanomas, which may have played a role in tumour growth inhibition by Asc, it was found not to affect the EPA content of tissues. Enhanced PG or LT synthesis and lipid perox:idation did not appear to have been involved in the tumour growth inhibitory interaction between Asc and the EP As/PUP As. THe activity of the enzyme delta-6-desaturase, a key enzyme in EF A metabolism which catalyses the desaturation of LA to GLA, and the influence of Asc on activity of the enzyme were also examined. The cultured cells, and BL6 cells in particular, were found to contain significant activity of the enzyme. Whereas murine liver microsomal fractions were found to contain delta-6-desaturase activity, microsomes from melanomas grown in mice were found to lack activity of the enzyme. The significant tumour promoting effects of the GLA containing EPO diet may have been the result of the lack of delta-6-desaturase activity in tumour cells grown in mice. Asc was found to stimulate activity of the enzyme in cultured BL6 cells but not in LLCM.K cells, while dietary Asc and EF A/PUP A manipulation did not influence activity of the enzyme in microsomal fractions. This study has confirmed previous reports of the in vivo tumour promoting effects of dietary EP As/PUP As and the tumour growth inhibitory effects of Asc. The in vitro cell growth inhibitory effects of Asc and the EP As also confirm the results of previous reports. Previous studies investigating possible interactions between Asc and EP As/PUP As in influencing tumour cell growth could not be located in the relevant literature. This study may therefore be one of the first investigations of any such interaction between these nutrients in tumour cells. While this study was not able to identify the mechanisms involved in the different tumour promoting or tumour growth inhibitory effects of the two nutrients in the two systems, it did eliminate a number of potential mechanisms. The results of this study also emphasise the difficulty of attempting to compare the results of in vitro and in vivo studies.
- Full Text:
- Date Issued: 1990
Interrupt-generating active data objects
- Authors: Clayton, Peter Graham
- Date: 1990
- Subjects: Parallel programming (Computer science) Electronic data processing -- Distributed processing
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4677 , http://hdl.handle.net/10962/d1006700
- Description: An investigation is presented into an interrupt-generating object model which is designed to reduce the effort of programming distributed memory multicomputer networks. The object model is aimed at the natural modelling of problem domains in which a number of concurrent entities interrupt one another as they lay claim to shared resources. The proposed computational model provides for the safe encapsulation of shared data, and incorporates inherent arbitration for simultaneous access to the data. It supplies a predicate triggering mechanism for use in conditional synchronization and as an alternative mechanism to polling. Linguistic support for the proposal requires a novel form of control structure which is able to interface sensibly with interrupt-generating active data objects. The thesis presents the proposal as an elemental language structure, with axiomatic guarantees which enforce safety properties and aid in program proving. The established theory of CSP is used to reason about the object model and its interface. An overview is presented of a programming language called HUL, whose semantics reflect the proposed computational model. Using the syntax of HUL, the application of the interrupt-generating active data object is illustrated. A range of standard concurrent problems is presented to demonstrate the properties of the interrupt-generating computational model. Furthermore, the thesis discusses implementation considerations which enable the model to be mapped precisely onto multicomputer networks, and which sustain the abstract programming level provided by the interrupt-generating active data object in the wider programming structures of HUL.
- Full Text:
- Date Issued: 1990
- Authors: Clayton, Peter Graham
- Date: 1990
- Subjects: Parallel programming (Computer science) Electronic data processing -- Distributed processing
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4677 , http://hdl.handle.net/10962/d1006700
- Description: An investigation is presented into an interrupt-generating object model which is designed to reduce the effort of programming distributed memory multicomputer networks. The object model is aimed at the natural modelling of problem domains in which a number of concurrent entities interrupt one another as they lay claim to shared resources. The proposed computational model provides for the safe encapsulation of shared data, and incorporates inherent arbitration for simultaneous access to the data. It supplies a predicate triggering mechanism for use in conditional synchronization and as an alternative mechanism to polling. Linguistic support for the proposal requires a novel form of control structure which is able to interface sensibly with interrupt-generating active data objects. The thesis presents the proposal as an elemental language structure, with axiomatic guarantees which enforce safety properties and aid in program proving. The established theory of CSP is used to reason about the object model and its interface. An overview is presented of a programming language called HUL, whose semantics reflect the proposed computational model. Using the syntax of HUL, the application of the interrupt-generating active data object is illustrated. A range of standard concurrent problems is presented to demonstrate the properties of the interrupt-generating computational model. Furthermore, the thesis discusses implementation considerations which enable the model to be mapped precisely onto multicomputer networks, and which sustain the abstract programming level provided by the interrupt-generating active data object in the wider programming structures of HUL.
- Full Text:
- Date Issued: 1990
Isolation of and interaction of nutrients with the linoleoyl-coa desaturase complex
- Authors: Perkins, Denise Mary
- Date: 1990
- Subjects: Cell proliferation , Cancer cells -- Growth -- Regulation , Enzymes -- Purification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4558 , http://hdl.handle.net/10962/d1018264
- Description: The termina1 enzyme in the linoleoyl-CoA desaturase enzyme complex, delta-6-desaturase was implied in the control of cell proliferation in cancer cells. One of the aims of this study was to isolate the terminal enzyme. It was decided that in order to isolate this enzyme it was first necessary to isolate the entire complex and then to enzymatically solubilise the first two components of the complex i e cytochrome b5 reductase and cytochrome b5 from the complex resulting in a pure delta-6-desaturase . The first two components were isolated and purified using simplified and easily reproducible methodologies which could be utilised in the final purification of delta-6- desaturase. The entire enzyme complex, linoleoyl-CoA desaturase was also isolated in a pure form and this pure complex was used to attempt to isolate delta-6-desaturase. The terminal enzyme was isolated with some cytochrome b5 still bound to it. The methods used had proven to be successful and with some modifications should yield a pure enzyme. Zinc and GLA were known to play a role in the inhibition of cancer cell proliferation and zinc was hypothesised to inhibit cell growth by stimulating the activity of the linoleoyl-CoA desaturase enzyme complex which is involved in the regulation of cell proliferation. GLA is the product of the reaction that this enzyme complex catalyses and GLA has been shown to inhibit cancer ce ll growth. The effect of GLA on cell growth and linoleoyl-CoA desaturase activity was thus investigated. Results showed that both zinc and GLA inhibited cell growth and that the combined addition of zinc and GLA generally resulted in the inhibition of cell growth and the activation of linoleoyl-CoA desaturase activity in the BL-6 cells while having a less pronounced effect on the LLCMK cells. The results of this study support the hypothesis that zinc may be a cofactor of linoleoyl-CoA desaturase.
- Full Text:
- Date Issued: 1990
- Authors: Perkins, Denise Mary
- Date: 1990
- Subjects: Cell proliferation , Cancer cells -- Growth -- Regulation , Enzymes -- Purification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4558 , http://hdl.handle.net/10962/d1018264
- Description: The termina1 enzyme in the linoleoyl-CoA desaturase enzyme complex, delta-6-desaturase was implied in the control of cell proliferation in cancer cells. One of the aims of this study was to isolate the terminal enzyme. It was decided that in order to isolate this enzyme it was first necessary to isolate the entire complex and then to enzymatically solubilise the first two components of the complex i e cytochrome b5 reductase and cytochrome b5 from the complex resulting in a pure delta-6-desaturase . The first two components were isolated and purified using simplified and easily reproducible methodologies which could be utilised in the final purification of delta-6- desaturase. The entire enzyme complex, linoleoyl-CoA desaturase was also isolated in a pure form and this pure complex was used to attempt to isolate delta-6-desaturase. The terminal enzyme was isolated with some cytochrome b5 still bound to it. The methods used had proven to be successful and with some modifications should yield a pure enzyme. Zinc and GLA were known to play a role in the inhibition of cancer cell proliferation and zinc was hypothesised to inhibit cell growth by stimulating the activity of the linoleoyl-CoA desaturase enzyme complex which is involved in the regulation of cell proliferation. GLA is the product of the reaction that this enzyme complex catalyses and GLA has been shown to inhibit cancer ce ll growth. The effect of GLA on cell growth and linoleoyl-CoA desaturase activity was thus investigated. Results showed that both zinc and GLA inhibited cell growth and that the combined addition of zinc and GLA generally resulted in the inhibition of cell growth and the activation of linoleoyl-CoA desaturase activity in the BL-6 cells while having a less pronounced effect on the LLCMK cells. The results of this study support the hypothesis that zinc may be a cofactor of linoleoyl-CoA desaturase.
- Full Text:
- Date Issued: 1990
Radio studies of ionized hydrogen in the southern Milky Way
- Authors: Gaylard, Michael John
- Date: 1990
- Subjects: Interstellar hydrogen Milky Way Astrophysics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5440 , http://hdl.handle.net/10962/d1001993
- Description: This thesis describes the results of a survey of the HI42ɑ recombination line emission at 2.3 GHz from HII regions in the Southern Milky Way, carried out with the 26 m diameter Hartebeesthoek radio telescope. The Galactic Longitude range covered was 290° to 40°. Single recombination lines were detected from 375 positions. Multiple lines were observed towards 90 positions in the inner Galaxy. No line emission could be detected in 28 positions. Continuum antenna temperatures were estimated from drift scans or radio maps observed for the purpose. LTE electron temperatures and turbulent velocities of the HII regions were calculated where possible. The properties of the sample were compared to those observed in HI09ɑ surveys. The lines observed from over 50 positions were first detections, of which half were associated with optically-identified HII regions. In about 150 cases the lines were only the second to be detected from those HII regions. The processes of the radio emission, detection, and analysis were simulated numerically. The detectability of the emission and the magnitude of non-LTE effects and pressure-broadening in multi-component HII regions was predicted and compared to observations. The radio luminosity function of the HII regions was determined over a range of three orders of magnitude in intrinsic brightness for the first time, using techniques which corrected for different types of incompleteness in the samples. The luminosity function was compared to those in five selected spiral galaxies, and shown to lie between those of M33 and M81. An alternate form of the luminosity function was developed for use with a numerical model of the spiral arm structure of the Milky Way. The physical parameters defining the major spiral arms were established by comparing synthesized diagrams of radial velocity versus Galactic Longitude with those actually observed. The faint, extended HII regions S9 and RCW129 in Scorpius, the Barnard Loop in Orion, and S296 in Canis Major were analyzed, using all available data. All the recombination lines from these HII regions were first detections
- Full Text:
- Date Issued: 1990
- Authors: Gaylard, Michael John
- Date: 1990
- Subjects: Interstellar hydrogen Milky Way Astrophysics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5440 , http://hdl.handle.net/10962/d1001993
- Description: This thesis describes the results of a survey of the HI42ɑ recombination line emission at 2.3 GHz from HII regions in the Southern Milky Way, carried out with the 26 m diameter Hartebeesthoek radio telescope. The Galactic Longitude range covered was 290° to 40°. Single recombination lines were detected from 375 positions. Multiple lines were observed towards 90 positions in the inner Galaxy. No line emission could be detected in 28 positions. Continuum antenna temperatures were estimated from drift scans or radio maps observed for the purpose. LTE electron temperatures and turbulent velocities of the HII regions were calculated where possible. The properties of the sample were compared to those observed in HI09ɑ surveys. The lines observed from over 50 positions were first detections, of which half were associated with optically-identified HII regions. In about 150 cases the lines were only the second to be detected from those HII regions. The processes of the radio emission, detection, and analysis were simulated numerically. The detectability of the emission and the magnitude of non-LTE effects and pressure-broadening in multi-component HII regions was predicted and compared to observations. The radio luminosity function of the HII regions was determined over a range of three orders of magnitude in intrinsic brightness for the first time, using techniques which corrected for different types of incompleteness in the samples. The luminosity function was compared to those in five selected spiral galaxies, and shown to lie between those of M33 and M81. An alternate form of the luminosity function was developed for use with a numerical model of the spiral arm structure of the Milky Way. The physical parameters defining the major spiral arms were established by comparing synthesized diagrams of radial velocity versus Galactic Longitude with those actually observed. The faint, extended HII regions S9 and RCW129 in Scorpius, the Barnard Loop in Orion, and S296 in Canis Major were analyzed, using all available data. All the recombination lines from these HII regions were first detections
- Full Text:
- Date Issued: 1990
Students' conceptions of simple D.C. electricity circuits: a study of primary, inappropriate conceptions, learning difficulties of physics students, and implications for instruction
- Authors: Jiya, Zindlovu
- Date: 1990
- Subjects: Physical sciences -- Study and teaching (Secondary) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5441 , http://hdl.handle.net/10962/d1001994
- Description: The work attempts to identify the general conceptual problems of the generations of Black students who pass through flrst year physics courses at the University of Fort Hare. In particular the alternative conceptions of students in the area of direct current electricity are investigated, using various techniques including written diagnostic tests. The main method used in the investigation of the student frameworks is the personal interview. A varied number of inappropriate conceptions are identifled in the students in significantly large proportions, and these are found to be dependent on many factors; for example the socio-cultural background like language and its metaphors, and media images. It is established that some of these are exacerbated by student perceptions about the nature of physics and of the scientific enterprise in general. Certain proposals are made about how to remedy the situation; relying mainly on the recently established innovative instructional strategies like conceptual change and cognitive conflict, and on making proposals about restructuring certain forms of presentation of the subject matter, paying attention to how language is used to address the speciflc problems of the students. The importance of providing practical experiences for the students is also emphasised
- Full Text:
- Date Issued: 1990
- Authors: Jiya, Zindlovu
- Date: 1990
- Subjects: Physical sciences -- Study and teaching (Secondary) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5441 , http://hdl.handle.net/10962/d1001994
- Description: The work attempts to identify the general conceptual problems of the generations of Black students who pass through flrst year physics courses at the University of Fort Hare. In particular the alternative conceptions of students in the area of direct current electricity are investigated, using various techniques including written diagnostic tests. The main method used in the investigation of the student frameworks is the personal interview. A varied number of inappropriate conceptions are identifled in the students in significantly large proportions, and these are found to be dependent on many factors; for example the socio-cultural background like language and its metaphors, and media images. It is established that some of these are exacerbated by student perceptions about the nature of physics and of the scientific enterprise in general. Certain proposals are made about how to remedy the situation; relying mainly on the recently established innovative instructional strategies like conceptual change and cognitive conflict, and on making proposals about restructuring certain forms of presentation of the subject matter, paying attention to how language is used to address the speciflc problems of the students. The importance of providing practical experiences for the students is also emphasised
- Full Text:
- Date Issued: 1990
The development of theology at Stellenbosch from 1859-1919
- Authors: Thom, Gideon
- Date: 1990
- Subjects: Nederduitse Gereformeerde Kerk , Stellenbosch Theological Seminary
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1215 , http://hdl.handle.net/10962/d1001544
- Description: This study seeks to give an exposition of the development of theology at the Stellenbosch Kweekskool from 1859 until 1919, a period that coincided with the formative years of Afrikaner nationalism. John Murray (1826-1882) was nurtured in evangelical Calvinism but received his theological training in 'moderate' Utrecht. As Calvinist he emphasized salvation by grace and Christian obedience, as evangelical, union with Christ, and as kenoticist he emphasized the relevance of the humanity of Christ. N.J. Hofmeyr (1827-1909) was converted under an evangelical Lutheran and received his training in Utrecht, in the heyday of Dutch ethical and German mediating theology. He was fascinated by the historical Jesus. In his view, the 'religion of Jesus' was not the moralistic one imagined by the modernists, but an experience of sonship, continually challenged by severe temptations. The central theme of his theology was the huiothesia, the sonship of Jesus being the prototype of our sonship. As he was the spiritual father of many generations of Stellenbosch students, his Christocentric emphasis and his doctrine of huiothesia played an important role in the development of NGK spirituality. Other themes in Hofmeyr's theology bore the marks of mediating theology: The idea that the conscience is the voice of God, the 'natural' compatibility between the human and the divine, the importance of experience in the theological enterprise and the idea that grace must accommodate itself to nature. J.I. Marais (1848-1919) and P.J.G. de Vos (1842-1931), who received part of their training in Scotland, were more conservative in theology than Hofmeyr. By the turn of the century De Vos has fully accepted scholastic Calvinism and premillenniaIism; Marais accepted premillennialism around 1914. The experiences of the Anglo-Boer War precipitated the close relationship between the Afrikaner and the NGK, and the lack of a strong doctrine of the church in Stellenbosch theology added to the blurring of distinctions between church and people. The national church of the Scots and Dutch traditions became the volkskerk of the Afrikaner. Important biblical dimensions of Murray and Hofmeyr's theology were neglected after Hofmeyr's death. The conviction that God revealed himself completely in the human Christ was weakened by fundamentalism and the tendency to see God's will in the history of the volk. Their eschatology - which expected great success for the gospel - was replaced by premillennialism, which expected only limited success for missions before the parousia. Hofmeyr's social gospel, which was applied to the problem of the 'poor whites', was gradually replaced by a negative view of the social relevance of the gospel, especially in racial matters. This gradual change of theological direction involved a growing sympathy with fundamentalism and Kuyperianism, but did not consist in a full revival of reformation theology
- Full Text:
- Date Issued: 1990
- Authors: Thom, Gideon
- Date: 1990
- Subjects: Nederduitse Gereformeerde Kerk , Stellenbosch Theological Seminary
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1215 , http://hdl.handle.net/10962/d1001544
- Description: This study seeks to give an exposition of the development of theology at the Stellenbosch Kweekskool from 1859 until 1919, a period that coincided with the formative years of Afrikaner nationalism. John Murray (1826-1882) was nurtured in evangelical Calvinism but received his theological training in 'moderate' Utrecht. As Calvinist he emphasized salvation by grace and Christian obedience, as evangelical, union with Christ, and as kenoticist he emphasized the relevance of the humanity of Christ. N.J. Hofmeyr (1827-1909) was converted under an evangelical Lutheran and received his training in Utrecht, in the heyday of Dutch ethical and German mediating theology. He was fascinated by the historical Jesus. In his view, the 'religion of Jesus' was not the moralistic one imagined by the modernists, but an experience of sonship, continually challenged by severe temptations. The central theme of his theology was the huiothesia, the sonship of Jesus being the prototype of our sonship. As he was the spiritual father of many generations of Stellenbosch students, his Christocentric emphasis and his doctrine of huiothesia played an important role in the development of NGK spirituality. Other themes in Hofmeyr's theology bore the marks of mediating theology: The idea that the conscience is the voice of God, the 'natural' compatibility between the human and the divine, the importance of experience in the theological enterprise and the idea that grace must accommodate itself to nature. J.I. Marais (1848-1919) and P.J.G. de Vos (1842-1931), who received part of their training in Scotland, were more conservative in theology than Hofmeyr. By the turn of the century De Vos has fully accepted scholastic Calvinism and premillenniaIism; Marais accepted premillennialism around 1914. The experiences of the Anglo-Boer War precipitated the close relationship between the Afrikaner and the NGK, and the lack of a strong doctrine of the church in Stellenbosch theology added to the blurring of distinctions between church and people. The national church of the Scots and Dutch traditions became the volkskerk of the Afrikaner. Important biblical dimensions of Murray and Hofmeyr's theology were neglected after Hofmeyr's death. The conviction that God revealed himself completely in the human Christ was weakened by fundamentalism and the tendency to see God's will in the history of the volk. Their eschatology - which expected great success for the gospel - was replaced by premillennialism, which expected only limited success for missions before the parousia. Hofmeyr's social gospel, which was applied to the problem of the 'poor whites', was gradually replaced by a negative view of the social relevance of the gospel, especially in racial matters. This gradual change of theological direction involved a growing sympathy with fundamentalism and Kuyperianism, but did not consist in a full revival of reformation theology
- Full Text:
- Date Issued: 1990
The Eucharist and history
- Paterson, Torquil John Macleod
- Authors: Paterson, Torquil John Macleod
- Date: 1990
- Subjects: Lord's Supper , History -- Religious aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1311 , http://hdl.handle.net/10962/d1018262
- Description: The thesis delineates an existential view of history, in which the eternal is defined as the ground of authentic human life which underlies true historical action. The historical is the manifestation of the eternal in the unique moment, and redefines the ahistorical conditions of human life. The ahistorical is the social and ideological conditioning of all human knowledge, usually presented in terms of various kinds of myth and ritual . The ahistorical contains both good and bad elements, but always has the tendency to become oppressive and is therefore constantly in conflict with the historical. The life of Jesus is described as the perfect expression of the eternal in true historical action, by which he came into conflict with the ahistorical of his society, as expressed in his death. By his resurrection, his life breaks the limitations of time and becomes transformative enabling all subsequent historical action. The eucharist is described as engaging with each of these dimensions of our existence. By being itself a ritual action containing a myth, the eucharist has an ahistorical form and therefore easily engages with the ahistorical dimensions of society. However, without a constant dialogue with the historical, the eucharist, as an ahistorical medium, can become allied to the dominant forces of society and become a means of oppression. The eucharist has at its centre the remembrance of the historical action of Jesus. True historical action in the present will result from a proper hermeneutic of the gospels. The eucharistic anamnesis must be regarded as part of the wider search for a relevant contemporary christology. The eucharist remembers the Last Supper, which is a parable of the whole life of Jesus and a prelude to his death and is a sacrifice in that it has a sacrificial form, and leads to our historical action, which will usually take the form of a conflict with the ahistorical and have sacrificial dimensions. The eternal only becomes present in our historical action, but the eucharist, by uniting us with the transforming power of the death and resurrection of Jesus, is a powerful aid to such action. The eucharist also provides the opportunity for resonances between Jesus and the ground of our being, thus enabling deep shifts of attitude and consciousness. Three fundamental prerequisites for human life are isolated and related to the eucharist: belonging, nurturing and giving. In order for the eucharist to ennable historical action is must hold these dimensions in tension. In its actual form it does this through the balance between the Words of Institution and the Epiclesis, which, in turn, provide the christological ground of the eucharist and relate this to the present through a particular pneumatology. The real presence is described by the thesis in a way which connects the eucharistic presence with the historical Jesus and leads to our historical action. Finally, some consequences of the thesis for Eucharistic practice are suggested. The relationship between the ahistorical form of the eucharist and the anamnesis is important. In this way the eucharist objectifies the ahistorical, reflects on this in terms of the historical action of Jesus, and reforms the ahistorical by modelling a response. This should lead to a more authentic expression of the eternal in the contemporary world
- Full Text:
- Date Issued: 1990
- Authors: Paterson, Torquil John Macleod
- Date: 1990
- Subjects: Lord's Supper , History -- Religious aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1311 , http://hdl.handle.net/10962/d1018262
- Description: The thesis delineates an existential view of history, in which the eternal is defined as the ground of authentic human life which underlies true historical action. The historical is the manifestation of the eternal in the unique moment, and redefines the ahistorical conditions of human life. The ahistorical is the social and ideological conditioning of all human knowledge, usually presented in terms of various kinds of myth and ritual . The ahistorical contains both good and bad elements, but always has the tendency to become oppressive and is therefore constantly in conflict with the historical. The life of Jesus is described as the perfect expression of the eternal in true historical action, by which he came into conflict with the ahistorical of his society, as expressed in his death. By his resurrection, his life breaks the limitations of time and becomes transformative enabling all subsequent historical action. The eucharist is described as engaging with each of these dimensions of our existence. By being itself a ritual action containing a myth, the eucharist has an ahistorical form and therefore easily engages with the ahistorical dimensions of society. However, without a constant dialogue with the historical, the eucharist, as an ahistorical medium, can become allied to the dominant forces of society and become a means of oppression. The eucharist has at its centre the remembrance of the historical action of Jesus. True historical action in the present will result from a proper hermeneutic of the gospels. The eucharistic anamnesis must be regarded as part of the wider search for a relevant contemporary christology. The eucharist remembers the Last Supper, which is a parable of the whole life of Jesus and a prelude to his death and is a sacrifice in that it has a sacrificial form, and leads to our historical action, which will usually take the form of a conflict with the ahistorical and have sacrificial dimensions. The eternal only becomes present in our historical action, but the eucharist, by uniting us with the transforming power of the death and resurrection of Jesus, is a powerful aid to such action. The eucharist also provides the opportunity for resonances between Jesus and the ground of our being, thus enabling deep shifts of attitude and consciousness. Three fundamental prerequisites for human life are isolated and related to the eucharist: belonging, nurturing and giving. In order for the eucharist to ennable historical action is must hold these dimensions in tension. In its actual form it does this through the balance between the Words of Institution and the Epiclesis, which, in turn, provide the christological ground of the eucharist and relate this to the present through a particular pneumatology. The real presence is described by the thesis in a way which connects the eucharistic presence with the historical Jesus and leads to our historical action. Finally, some consequences of the thesis for Eucharistic practice are suggested. The relationship between the ahistorical form of the eucharist and the anamnesis is important. In this way the eucharist objectifies the ahistorical, reflects on this in terms of the historical action of Jesus, and reforms the ahistorical by modelling a response. This should lead to a more authentic expression of the eternal in the contemporary world
- Full Text:
- Date Issued: 1990
The observation of extended sources with the Hartebeesthoek radio telescope
- Authors: Mountfort, Peter Ian
- Date: 1990
- Subjects: Radio telescopes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5479 , http://hdl.handle.net/10962/d1005265
- Description: The Hartebeesthoek Radio Telescope is well suited to mapping large areas of sky at 2.3 GHz because of the stability and sensitivity of the noise-adding radiometer (Nicolson, 1970) and cryogenic amplifier used at this frequency, the relatively large 20' beam of the 26 m dish antenna, and its high-speed drive capability. Telescope control programs were written for the Observatory's online computer for automated mapping. Effort centred on removing the curved baseline or 'background' from each Declination (Dec) scan, due to atmospheric and ground radiation contributions varying as the antenna is scanned. Initially these backgrounds were measured over a wide range of Hour Angle (HA) for the Dec range of a map, and an interpolated curve subtracted from each on-source scan for its HA. A common base level was established by comparison with drift scans (observed with the antenna stationary). These different observations (on- and off-source Dec scans and drift scans) were combined into one in the Skymap system by performing Dec scans at a fixed starting HA for a period long enough to permit 'cold sky' and the source to drift through. A background formed by fitting a smooth curve through the lowest sample at each Dec provides a consistent relative base level for all the scans in an observation. A high scanning speed is used so that observations may fruitfully be repeated three times and interleaved to build a reliable, fully sampled map. As each observation has its own background removed, it may be made at any HA. For comparison, maps of Upper Scorpio produced by the earlier method (Baart et al., 1980) and the Magellanic Cloud region produced by Skymap (Mountfort et al., 1987) are shown. Skymap provides a simple and flexible mapping method which relies on the stability of the noise-adding radiometer and high-speed repeated scans to produce good maps of large or small extent with little computation. Correction for drift is more difficult than with systems which use intersecting scans, such as the 'nodding' scans used by Haslam et al. (1981) or the Azimuth scans of Reich (1982).
- Full Text:
- Date Issued: 1990
- Authors: Mountfort, Peter Ian
- Date: 1990
- Subjects: Radio telescopes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5479 , http://hdl.handle.net/10962/d1005265
- Description: The Hartebeesthoek Radio Telescope is well suited to mapping large areas of sky at 2.3 GHz because of the stability and sensitivity of the noise-adding radiometer (Nicolson, 1970) and cryogenic amplifier used at this frequency, the relatively large 20' beam of the 26 m dish antenna, and its high-speed drive capability. Telescope control programs were written for the Observatory's online computer for automated mapping. Effort centred on removing the curved baseline or 'background' from each Declination (Dec) scan, due to atmospheric and ground radiation contributions varying as the antenna is scanned. Initially these backgrounds were measured over a wide range of Hour Angle (HA) for the Dec range of a map, and an interpolated curve subtracted from each on-source scan for its HA. A common base level was established by comparison with drift scans (observed with the antenna stationary). These different observations (on- and off-source Dec scans and drift scans) were combined into one in the Skymap system by performing Dec scans at a fixed starting HA for a period long enough to permit 'cold sky' and the source to drift through. A background formed by fitting a smooth curve through the lowest sample at each Dec provides a consistent relative base level for all the scans in an observation. A high scanning speed is used so that observations may fruitfully be repeated three times and interleaved to build a reliable, fully sampled map. As each observation has its own background removed, it may be made at any HA. For comparison, maps of Upper Scorpio produced by the earlier method (Baart et al., 1980) and the Magellanic Cloud region produced by Skymap (Mountfort et al., 1987) are shown. Skymap provides a simple and flexible mapping method which relies on the stability of the noise-adding radiometer and high-speed repeated scans to produce good maps of large or small extent with little computation. Correction for drift is more difficult than with systems which use intersecting scans, such as the 'nodding' scans used by Haslam et al. (1981) or the Azimuth scans of Reich (1982).
- Full Text:
- Date Issued: 1990