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Design of Immunobiosensors for Detection of Tumor-Associated Anti-P53 Autoantibodies: Method Development

- Adeniyi, Omotayo Kayode

Authors: Adeniyi, Omotayo Kayode
Date: 2020
Subjects: Uncatalogued
Language: English
Type: thesis , text , Doctoral , PhD
Identifier: http://hdl.handle.net/10962/162988 , vital:41002 , 10.21504/10962/162988
Description: Introduction -- Experimental -- Label-Free Impedimetric Sensing of Anti-P53ab... -- Fluorescent detection of Anti-P53ab -- Peroxidase-like activity of Fe3O4@SiNP-APTES-Au@Pd... -- Colorimetric detection of Anti-P53ab , Thesis (PhD)--Rhodes University, Science Faculty, Department of Chemistry, 2020. , Detection and profiling of circulating tumor-associated autoantibodies (TAAbs) are useful for screening and early-stage diagnosis of asymptomatic lung cancer. Immunobiosensor technologies aimed to accomplish the highly sensitive, rapid and low-cost detection of TAAbs can improve the early-stage detection of lung cancer. Immunobiosensors for the detection of anti-P53-tumour associated autoantibodies have been developed in this work. The design of sensing interfaces with immobilized P53 protein (P53ag) as a sensing element layer on a solid interface was investigated. Several methods of detecting anti-P53-antibodies (anti-P53ab) were investigated. These methods are label-free detection using electrochemical impedance spectroscopy (EIS) and two label techniques. The label-free electrochemical techniques utilize gold electrode pre-modified with a conducting layer of electrochemically grafted phenylethylamine for covalent immobilization of P53ag. The limit of anti-P53ab detection with the label-free EIS was 103.0 pg.ml-1. The labeled technique developed utilizes fluorescent, and peroxidase-like nanomaterial labeled antibody as a detection probe. For the fluorescence detection, fluorescent silica nanoparticles were synthesized by overloading FITC into the silica matrix and conjugated to detection antibody (anti-IgG). The detection of the anti-P53ab was based on the dissolution of the silica nanoparticles to release the loaded dye as a signal amplification strategy. The fluorescence detection was carried out on a microplate, and magnetic bead modified P53-antigen platforms and limit of detection (LoD) were 42.0 fg.ml-1 and 3.3 fg.ml-1 for anti-P53ab; respectively. Fe3O4@SiNP-APTES-Au@Pd hybrid nanoparticles were synthesized, and their peroxidase-like activity and colorimetric detection were evaluated. The Fe3O4@SiNP-APTES-Au@Pd exhibited comparable activity to HRP. The Fe3O4@SiNP-APTES-Au@Pd was conjugated to protein-G-anti-IgG for the detection of anti-P53ab on a microplate and cellulose paper platforms. The LoD was 20.0 fg.ml-1 and 63.0 fg.ml-1 for the microplate and cellulose paper platform; respectively. The potential application of the designed immunobiosensor was evaluated in simulated serum samples. The developed sensors showed higher detection sensitivity, stability and had a lower detection limit for anti-P53ab when compared with the ELISA based detection. The results have provided alternative and effective quantification approaches to ELISA and a promising future for multiplexed detection of tumor-associated autoantibodies. The developed methodologies in this thesis could be applied for the detection of other autoantibodies in other cancer types and auto-immune diseases.
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Date Issued: 2020

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The design, synthesis and antiplasmodial activity of a series of halogenated fosmidomycin analogues and hybrid drugs

- Afolayan, Anthonia Folake

Authors: Afolayan, Anthonia Folake
Date: 2012
Subjects: Uncatalogued
Language: English
Type: text , Thesis , Doctoral , PhD
Identifier: http://hdl.handle.net/10962/64370 , vital:28538
Description: Malaria continues to be a devastating disease and a major cause of death in sub-Saharan Africa. With resistance against most of the available antimalarial drugs, there is a need for ongoing research and development of antimalarial agents. Fosmidomycin and its acetyl analogue FR900098 have been identified as potent inhibitors of Plasmodium falciparum, the causative agent of the most deadly form of malaria. Clinical trials of these agents have revealed poor absorption due to their high hydrophilicity. In the present studies the effect of halogenation of the acyl chain as well as the biological effect of extending the acyl sidechain was explored. This provided the basis on which fosmidomycin hybrids were designed to investigate the feasibility of hybrid extending into NADPH binding pocket. Synthesis of a series of halogenated FR900098 analogues was carried out in three stages. This included i) The introduction of the phosphonate group by reaction with 1,3dibromopropane in an Arbuzov reaction, ii) The introduction of a hydroxamate group by reaction of the propyl phosphonate by means of a nucleophilic substitution reaction with BocNHOBn and iii) The introduction of a halogenated acyl side chain on a protected fosmidomycin backbone. The synthesis of fosmidomycin-hybrids for which chloroquinefosmidomycin hybrids were used as the prototype, involved convergence of the two separately constructed moieties i.e. fosmidomycin and the quinoline moieties in a covalent linkage. The quinoline moiety was easily synthesized from the reaction of 4,7dichloroquinoline with 1,2-diamino ethane. The aminoquinoline so formed resulted in chloroquine-fosmidomycin hybrids 3.8 and 3.9 when reacted with halogenated FR900098 analogues. Antiplasmodial assays were conducted on the chloroquine-fosmidomycin hybrids and the halogenated fosmidomycin derivatives against the chloroquine resistant Gambian FCR-3 strain of P. falciparum. The most potent iodoacetyl fosmidomycin analogues 2.21 gave an IC50 value of 5.54 µM which is eight times more potent than the known antiplasmodial FR900098 which gave an IC50 value of 41.67 µM. All the halogenated FR900098 analogues showed better antiplasmodial activity than their non-halogenated derivatives. This indicated that the presence of halogens in the FR900098 analogues contributes to their biological Chapter 1 Literature review activity. The acetyl and propyl linked hybrids 3.8 and 3.9 showed potent antiplasmodial activity with IC50 values of 0.18 and 0.82 µM respectively. These were by far the most potent hybrids synthesized and provided leads for a new class of promising antimalarial agents. Preliminary E. coli DXR enzyme inhibition assays were carried out on the halogenated fosmidomycin analogues. The results showed good inhibition of the enzyme by the phosphonic acids of the chloroacetyl and chloropropyl analogues 2.1 and 2.2 respectively. Molecular modelling of the compounds on E. coli (PDB code: 2EGH) and P. falciparum (PDB code: 3AUA) DXR showed strong binding of the halogenated fosmidomycin analogues while the hybrids in the absence of docked NADPH showed minimum binding to the enzymes.
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Date Issued: 2012

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Macroplastics in the environment: are they suitable habitats for macroinvertebrates in riverine systems?

- Ali, Andrew Abagai

Authors: Ali, Andrew Abagai
Date: 2023-10-13
Subjects: Uncatalogued
Language: English
Type: Academic theses , Master's theses , text
Identifier: http://hdl.handle.net/10962/424153 , vital:72128
Description: Emerging pollutants, such as plastics are threat to freshwater ecosystems, and may negatively impact riverine systems. They can modify riverine habitats and affect aquatic organism distribution and composition. Knowledge of how macroplastics alter riverine habitat heterogeneity, and their effects on macroinvertebrate assemblage structure is sparse, especially in Africa. This study examines the effect of hydraulic biotopes on the colonisation, establishment and succession patterns of macroinvertebrates on macroplastic and natural substrates based on the taxonomic and trait-based approach. Four experimental sites from minimally impacted upper reaches of the Buffalo, Kat, Kowie, and Swartkops Rivers in the Eastern Cape of South Africa were selected for the deployment of plastic substrates. Plastics materials, including polyethylene terephthalate (PET) bottles and natural substrate composed of stone and vegetation, were used to formulate three substrate groups: Group 1: 100% natural substrates (NS), Group 2: 50% natural substrates and 50% plastic material (NP), and Group 3: 100% plastic materials (PD). These substrates were placed in litter bags of equal dimension (25 cm by 35 cm, with 2.5 cm mesh) and deployed randomly in three hydraulic biotopes (pools, riffles, runs) over a period of 180 days (October 2021 to April 2022). A total of 216 substrate bags, 54 bags per substrate were deployed per site in the four experimental sites. Twelve bags from each substrate group were retrieved at an interval of 30 days beginning on day 30 after deployment, and analysed for the establishment of macroinvertebrate communities. Based on composite hydraulic biotope data, Simpson index was significantly higher (P < 0.05) for macroinvertebrate assemblage structure on the 50% and 100% macroplastic substrate groups compared to natural substrates. With the exception of Tabanidae, Glossosomatidae, and Psephenidae, all macroinvertebrate taxa recorded showed non-significant positive correlations with all three substrate groups. However, Tabanidae, Glossosomatidae, and Psephenidae showed significant positive correlation with the 100% natural substrates, 50% plastic substrates and 100% plastic substrates, respectively. The parsimony analysis reveal that, within 30 days, all substrate groups underwent similar succession, with high abundance of pioneer taxa which increased on days 60 and 90, and then decreased from days 120 to 180. For the the pool biotope, Shannon and Simpson indices were significantly higher (P < 0.05) for the macroinvertabrates collected over the natural substates compared with those collected on the macroplastic substrate groups. However, in the riffle and run biotopes, all diversity indices were similar for all substrate groups and no statistically significant difference was observed. Statistically significant higher values for taxonomic richness, diversity, and evenness were found on day 30 to 90 for the riffle biotopes, and day 30 to 60 for the run biotopes. The run biotope presented temporal statistical significant variability in taxonomic composition with different macroinvertebrate communities recorded on days 30 and 60 compared with days 90 to 180. However, in pools and riffles, no temporal variation was observed in the taxonomic composition of macroinvertebrates on all three substrate groups. The trait-based fuzzy correspondence analysis revealed differential spatial-temporal distribution of macroinvertebrate traits on all three substrate group. The early colonisers i.e. day 30 – 60, were dominated by group of taxa characterised by medium (>10 – 20 mm) and large (20 > 40) body size, flat body, collector-gatherers, free-living, and predators. The late colonisers, collected mainly on day 150 and 180 were dominated by taxa with a preference for high flow velocity (0.3 - 0.6 m/s), permanent attachment, and filter-feeding mode. Traits such as oval and flat body shape, medium body size (>10 - 20 mm), skating and clinging/climbing mobility, temporal attachment, shredders, predators, prey, and plastron and spiracle respiration showed positive correlation with the 100% macroplastic substrates. Filter feeding, crawling, permanent attachment, a preference for fast velocity (0.3-0.6 m/s), and coarse particle organic matter were positively correlated with the 50% macroplastic substrates. Overall, the results provided critical insights on the impact of macroplastics on the assemblage structure of biological communities by acting as suitable habitats in stream ecosystems. The study elucidated the role of traits of aquatic organisms in mediating the colonisation of plastics substrates, providing insights into the impact of plastics proliferation on riverine ecosystem functioning. Furthermore, the finding provides a baseline insight into the influence of hydraulic biotopes on the colonisation and establishment of macroinvertebrates on macroplastic acting as artificial riverine habitat. , Thesis (MSc) -- Faculty of Science, Institute for Water Research, 2023
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Date Issued: 2023-10-13

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Associations between contraceptive use, physical activity, depression, and quality of life among women of childbearing age in Akure South Local Government area of Ondo State, Nigeria

- Alimi, Olabisi Ganiyat

Authors: Alimi, Olabisi Ganiyat
Date: 2023-10-13
Subjects: Uncatalogued
Language: English
Type: Academic theses , Master's theses , text
Identifier: http://hdl.handle.net/10962/424210 , vital:72133
Description: Background: Population experts and policymakers are concerned about Nigeria's rapid annual population growth due to the country's high birth rate which was 5.3 births per woman in 2018. Fear of the side effects of modern contraceptives among Nigerian women contributes to the low rate of contraceptive use, which was reported to be 17% and 37% among married and sexually active unmarried women, respectively. Which is a significant cause of the high birth rate. Although the side effects of modern contraceptives on women's clinical and physiological variables are well known, studies examining the associations between contraceptive use and physical and psychosocial variables such as physical activity (PA), depression, and quality of life (QoL) in non-athletic Nigerian women of reproductive age are scarce. This study aimed to examine the associations between contraceptive use, PA, depression, and QoL among non-athletic women of childbearing age in Akure South Local Government, Ondo State, Nigeria. Methods: In a descriptive cross-sectional study, 646 women of childbearing age were recruited using the multistage sampling technique. The data of 496 current contraceptive users and 146 non-users were analysed, as 4 respondents did not respond regarding current contraceptive use status. The Global PA Questionnaire (GPAQ), Beck’s Depression Inventory (BDI) and World Health QoL Organization Quality of Life Brief (WHOQoL BREF) were used to assess respondents’ scores/levels of PA, depression and health-related quality of life (HRQoL). The GPAQ, BDI, and WHOQOL scores were compared between contraceptive users and non-users using non-parametric Quade Analysis of Covariance while age, married status, tribe, religion, and occupation were included covariates. PA, sedentary behaviour (SB), and BDI scores were categorized using guidelines. Contraceptive use/practice was the outcome variable. Pearson's chi-square test bivariate analysis and a multivariate logistic model were used to identify factors associated with contraceptive use (users and non-users). Crude and adjusted odds ratios and their confidence intervals were calculated to determine the significance of the association. The regression model was adjusted for age, marital status, religion, tribe, highest education level, occupation, awareness of contraceptives, current use, lifetime use, type, class, and duration of current contraception. “Statistical significance was set at p < 0.05. Results: The mean age of the respondents was 29.73±6.10 years. The contraceptive users and non-users were not significantly different regarding their ages (p = 0.135), marital status (p = 0.245), highest education (p = 0.444), occupation (p = 0.238), and tribe (p = 0.192). The respondents’ lifetime and point prevalence of contraceptive uptake was 93.6% and 77.3%, respectively, while 72 (12.8%) reported experiencing contraception-related side effects. Of the 496 respondents who currently practice contraception, 146 (29.4%) were hormonal contraceptive users. The majority of the respondents had moderate and mild levels of PA (48.5%) and depression (51.4%), respectively, and a significantly higher proportion of contraceptive users had minimal and moderate depression levels than the non-users (p = 0.018). The contraceptive users demonstrated significantly higher median scores of BDI (p = 0.02), Physical health QoL (p < 0.001), environment QoL (p = 0.033) and overall QoL (0.004) than the non-users. Hormonal contraceptive users had significantly higher median PA walking/bicycling scores than non-hormonal users (p = 0.014). Respondents with mild and moderate depression levels had higher odds of being contraceptive users than those with minimal depression (AOR = 3.12, 95% CI = 1.43 – 6.80, p = 0.04 and 4.67, 95% CI = 1.92 – 11.36, p = 0.001 respectively). Conclusion: Contraceptive use is negatively associated with depression but positively related to Physical health, environment and overall domains of HRQoL. Healthcare professionals should consider women's mental and emotional condition while advising on family planning for optimal HRQoL. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
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Date Issued: 2023-10-13

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A phenomenological investigation of the beginning therapist's experience of the first session of psychotherapy with the first patient

- Allen, Jennifer Ann

Authors: Allen, Jennifer Ann
Date: 1987
Subjects: Uncatalogued
Language: English
Type: Master's theses , text
Identifier: http://hdl.handle.net/10962/193129 , vital:45301
Description: The aim of this project is to come to an understanding of how the situation of the first session of psychotherapy with the first patient is lived by the beginning therapist and what meaning this situation holds for him. The writer's interest in this phenomenon grew out of her own experience of this situation as a clinical Masters coursework student, an experience which was of important to the writer and meaningful still as a therapist in training. In dialogue with experienced therapists the importance of this situation was again made apparant. It was the opinion of these therapists that although for some the details of this experience had become dulled by time, what remained meaningful to them was that this experience was seen as the beginning of a project which remains important to them - they identified this situation as an important moment in the history of their development as psychotherapists. In the hope that the literature pertaining to psychotherapy would throw some light on this situation, the writer turned to a number of sources in this area to discover that no literature available to her elucidated this situation in a holistic manner. This led the writer to go back to the beginning therapists themselves so that they may speak for themselves of their experience of this situation. A phenomenological method of enquiry is implemented in this study as it renders the subject matter accessible to investigation, and allows it to reveal itself as it essentially is. This project is then an attempt to come to a general description of the beginning therapist's experience of the first session of psychotherapy with the first patient and thus to articulate the structure of the beginning therapist's lived situation (world) in this context. , Thesis (MA) -- Faculty of Humanities, Psychology, 1987
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Date Issued: 1987

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Thermal physiology of juvenile red roman seabream, Chrysoblephus laticeps after long-term exposure to low pH conditions

- Allison, Caitlin

Authors: Allison, Caitlin
Date: 2023-10-13
Subjects: Uncatalogued
Language: English
Type: Academic theses , Master's theses , text
Identifier: http://hdl.handle.net/10962/424323 , vital:72143
Description: Climate change has caused a combination of effects on the physiology of fishes. Of particular concern are the effects of thermal variability and ocean acidification. Organismal energy budgets change throughout ontogeny and research into the metabolic scope during early life stages is particularly useful in identifying potential bottlenecks. The first part of this thesis aimed to assess the absolute aerobic scope (AAS, described as the difference between the maximum and standard metabolic rates) of individual juveniles from a protected population of the endemic, commercially important seabream, Chrysoblephus laticeps, across a range of ecologically relevant temperatures (T = 11, 14, 18, 22˚C) under present-day conditions (pH = 8.03, pCO2 ≈ 420 μatm) using intermittent flow respirometry. The second component sought to investigate how long-term exposure (from fertilisation to juvenile, ~100 days exposure) to high-pCO2/hypercapnic conditions (pH = 7.63, pCO2 ≈ 1400 μatm), would affect the AAS of juvenile C. laticeps over a range of temperatures. Lower pH conditions were predicted to cause a decrease in the AAS of treatment animals due to additional energetic costs of acid-base regulation. The findings of the first data chapter demonstrated that juvenile C. laticeps reared under current CO2 conditions are tolerant to a wide range of thermal conditions, and individuals with a broad aerobic scope will be the best suited to coping with enhanced thermal variability. In contrast to the expected outcomes of the second data chapter, juvenile C. laticeps reared under high pCO2 conditions displayed greater AAS at high and low temperatures when compared with specimens from high pH conditions. Whilst a high degree of individual phenotypic variation was observed in the metabolic response of both groups, this was reduced at the lower and upper extreme temperatures for high pH and low pH animals respectively. Notably, the variation in treatment animal’s SMR was significantly diminished across all temperatures tested, compared to only a localised reduction in the SMR of high pH animals at cold temperatures. This may be indicative of compensatory pathways affecting energy restructuring and thermally-governed physiological trade-offs under hypercapnia. Given these results, juvenile C. laticeps appear to be more resilient to ocean acidification than anticipated, potentially owing to intrapopulation metabolic phenotypic diversity. This is likely attributed to the parental lineage originating in the Tsitsikamma MPA, which is thought to boast greater phenotypic diversity as a consequence of the refuge that these conservation areas offer from exploitation. Owing to the restriction imposed by the availability of surviving, captive-reared juveniles, the sample size used in this study was relatively low. However, owing to the repeated-measures nature of this research the sample size was sufficient to offer suitable statistical power for the polynomial mixed model used in the analysis. Future research should incorporate both physiological and behavioural responses to multiple environmental stressors to better understand covariation between these two traits, and to detect any behavioural trade-offs that might arise through compensation. In addition, these trials should be repeated using offspring from outside of the MPA to compare whether the same level of resilience and metabolic phenotypic diversity would be present in an exploited population. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
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Date Issued: 2023-10-13

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Self-reports on the effectiveness of psychotherapy with therapists-in-training: an 18-month follow-up study at a psychology training clinic

- Angus, Catherine Clare

Authors: Angus, Catherine Clare
Date: 1993
Subjects: Uncatalogued
Language: English
Type: Master's theses , text
Identifier: http://hdl.handle.net/10962/193069 , vital:45295
Description: The purpose of this study is to ascertain whether the clients who make use of the services of the Psychology Clinic at Rhodes University perceive the masters students being trained at the Clinic as providing adequate psychotherapy. Using a sample of 18 clients, who underwent psychotherapy at the Rhodes Psychology Clinic during 1990, a follow-up study was conducted to ascertain the perceived success of that psychotherapy. Those variables most pertinent to the outcome of psychotherapy are examined. The study uses both qualitative and quantitative methodology and shows that, overall, 89% of the clients who participated in this study perceived the psychotherapy at the Rhodes Psychology Clinic to be satisfactory. The study also highlights those factors which were perceived to be negative in the psychotherapeutic equation and it is hoped that the findings can be beneficial in planning more efficient services for the Clinic in the future. , Thesis (MA) -- Faculty of Humanities, Psychology, 1993
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Date Issued: 1993

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South African traditional healers’ perceptions and treatment of the symptoms of depression

- Augustine, Daniella Joan

Authors: Augustine, Daniella Joan
Date: 2023-03-30
Subjects: Uncatalogued
Language: English
Type: Academic theses , Master's theses , text
Identifier: http://hdl.handle.net/10962/408789 , vital:70525
Description: While depression is experienced worldwide, the World Health Organization indicated that over 80% of this disease burden is found in low- and middle-income countries, with 16% of depressive disorder cases being found in Africa. Specifically, in South Africa, the lifetime prevalence of major depressive disorder is 9.7%. Research conducted on traditional healing estimates that as high as 80% of black individuals in South Africa choose to consult traditional healers. Although there was initial consensus that the use of traditional healers reflected a lack of access to alternative resources, more recent research has however indicated that traditional healers are consulted even when psychiatric facilities and medication are available. Research has illustrated that many patients that chose to consult traditional healers do so as a result of their shared beliefs, and traditional healers' ability to understand the cultural frameworks underlying illness. The current study investigated how traditional healers in the Eastern Cape perceive and treat phenomena currently understood as the symptoms of depression and how these understandings can be incorporated into collaborations between Western medicine and traditional healing practice. A qualitative research design was used to examine the perspectives and treatment methods of isiXhosa traditional healers in the Eastern Cape. Four healers participated in the study. Key findings indicated that there is no single cause or definition of the phenomenon of depression by healers. Causality is greatly attributed to spiritual causes and ancestors play a key role in guiding the diagnosis and multiple treatment interventions employed by healers to treat the experience of depression. Finally, all traditional healers expressed a willingness to collaborate and work with the Western healthcare system, however, they indicated a strong need for greater recognition as a valid healthcare system. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
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Date Issued: 2023-03-30

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The link between behavioural plasticity and aerobic scope 1 phenotypes in predicting the survival of Chrysoblephus laticeps 2 under climate variability

- Bailey, Lauren Ashleigh

Authors: Bailey, Lauren Ashleigh
Date: 2023-03-29
Subjects: Uncatalogued
Language: English
Type: Academic theses , Doctoral theses , text
Identifier: http://hdl.handle.net/10962/422606 , vital:71961 , DOI 10.21504/10962/422606
Description: Thermal variability in the marine environment is likely to have a considerable effect on fishes as it impacts physiological performance and vital (i.e metabolism, foraging and swimming style) and non-vital (i.e. reproductive fitness) energetic processes. When fish are subjected to thermal stress, they may primarily respond by changing their behaviour. Species that have broad phenotypic behavioural plasticity (i.e. defined as the ability to adjust behavioural activity in presiding environmental conditions in order to remain within their optimal thermal range) may have a competitive advantage. Fish behavioural plasticity may take many forms. Some species may seek out thermal refugia by changing their phenology or distribution, while others alter the timing of their seasonal and spawning migrations in response to a changing environment. Although fishes can use behavioural changes to cope with climate change impacts, there does appear to be variability in the behavioural responses within species. However, if alterations in behaviour are insufficient to ensure that the individual remains within their optimal thermal range, physiological acclimation (i.e. defined as the process in which an organism adjusts to prevailing conditions by broadening their thermal performance curve so that their performance is maximized in the new thermal environment) may be required. Therefore, there is a critical link between the behaviour and thermal physiology of fishes, particularly in a world where they are facing increasing thermal stress. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
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Date Issued: 2023-03-29

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Exploring senior phase teachers’ use of kahoot! Gamification as a formative assessment tool to scaffold mathematical understanding

- Balele, Silence

Authors: Balele, Silence
Date: 2024-04
Subjects: Uncatalogued
Language: English
Type: Academic theses , Master's theses , text
Identifier: http://hdl.handle.net/10962/423891 , vital:72102
Description: In South Africa, digital technology, particularly gamification, continues to become an important teaching and learning tool. Its importance lies in scaffolding mathematical understanding, improving the quality of assessment, and developing twenty-first-century technological skills for effective mathematics assessment. To explore this importance, senior phase mathematics teachers are encouraged to adopt gamification technology such as Kahoot! in assessment. The study adopted a case study research design with a interpretivist research paradigm, as this method allows detailed exploration and understanding of a specific context through a close connection with the participants. Data was collected using semi-structured questionnaires, focus group interviews, non-participant observations, and reflective journals. The research involved ten purposively selected senior-phase mathematics teachers from two schools in the Sarah Baartman District, Eastern Cape Province, South Africa. The study drew upon Vygoysky's Sociocultural Theory for its theoretical framework, while the Technological, Pedagogical, and Content Knowledge was used for analysis. The data analysis pursued an inductive approach. Adopting a thematic analysis included coding participant responses, organizing codes into concepts and categories, and developing a theoretical framework from the resultant categories. A four-phase process was involved: coding, conceptualizing, categorizing, and theorizing, was employed. This process and using NVivo software for data organization and management helped identify patterns, themes, and significant concerns surrounding the study's core subject. The findings from the study were as follows: Firstly, it was revealed that most teachers have a positive perception and attitude towards using Kahoot! for formative assessment, believing that it enhances teaching and learning quality. However, they showed scepticism due to insufficient Information and Communication Technologies knowledge and training. Second, teachers' pedagogical and technological experiences were predominantly enjoyable. Kahoot! was accepted as beneficial for promoting collaboration, interaction, and immediate feedback. Third, the study identified enabling and constraining factors in using Kahoot! for formative assessment. The enabling factors include Kahoot!, which was convenient and accessible, promoted immediate feedback and fostered collaboration and interaction. The constraining factors include Information and Communication Technologies and network interference, which posed limitations. The research concluded that Kahoot! as a platform for formative assessment indeed scaffold mathematical understanding. The study recommends increasing teachers' access to educational technology, incorporating continuous professional development programs, providing subject-specific training, and encouraging teachers to adapt to emerging technologies. The study also provided recommendations for future research to explore learner perceptions, employ a mixed methods approach to help quantify learner performance and investigate the pedagogical and technological experiences of senior phase mathematics teachers regarding formative assessment. , Thesis (MEd) -- Faculty of Education, Secondary and Post-School Education, 2024
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Date Issued: 2024-04

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The effect of piospheres on the ecology of insectivorous birds and their arthropod prey

- Balmer, Natasha Louise

Authors: Balmer, Natasha Louise
Date: 2023-10-13
Subjects: Uncatalogued
Language: English
Type: Academic theses , Master's theses , text
Identifier: http://hdl.handle.net/10962/424380 , vital:72148
Description: Desertification is the degradation of arid ecosystems that result in the loss of biodiversity. Piospheres are areas of local degradation around a central point due to overgrazing and increased herbivore presence. There is a paucity of information regarding the effect of localised degradation on arthropods and insectivorous birds. Both of these organisms play crucial roles in ecosystem functioning and stability and can be used as models to study ecosystem functioning. I investigated the effect of piospheres on arthropods and birds in the Eastern Cape province of South Africa. I found that the abundance and diversity of arthropods were significantly reduced inside the piospheres due to the lack of vegetation. Termites were a group specifically negatively impacted by piospheres, with a significant reduction in their presence inside the piosphere. The family composition of arthropods also changed inside and outside the piospheres, with Caelifera, Diptera and Formicidae being the most dominant groups. The diversity of birds was also significantly reduced due to the degradation inside the piospheres. Looking at insectivorous birds, I found that the reduction in both vegetation and arthropod prey availability resulted in non-random avoidance of piospheres. This shows that piospheres negatively impact both arthropods and birds. The results from my study are supported by other literature studying the effects of habitat degradation associated with desertification. Due to the similarities of degradation between piospheres and desertification I make the argument that piospheres can be studied as localised models of desertification. The decrease in vegetation and arthropod abundance and diversity was found to further impact the feeding success of insectivorous birds. Using piospheres as a model for desertification, I found that the foraging effort of birds is significantly reduced within a degraded area due to the lack of vegetation providing safety to arthropod prey species. In addition to this, the foraging efficiency of insectivorous birds is significantly reduced inside the piospheres due to the decreased arthropod abundance and diversity. This shows that inside the piospheres birds spend less time searching for insects and have fewer successful feeds. This has implications for desertification of arid environments where birds face hyperthermia. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
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Date Issued: 2023-10-13

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Making the personal political: understanding the impacts of participation in an anti-rape protest for women who have experienced sexual violence

- Barker, Kim Elise

Authors: Barker, Kim Elise
Date: 2023-03-29
Subjects: Uncatalogued
Language: English
Type: Academic theses , Doctoral theses , text
Identifier: http://hdl.handle.net/10962/422590 , vital:71960 , DOI 10.21504/10962/422590
Description: South Africa is recognised as a country with unusually high levels of rape and sexual violence. The majority of those who experience sexual violence do not acknowledge, disclose or report it, and do not access support. Many factors have been identified which can inhibit acknowledgement and disclosure. While silence is to be honoured as a chosen response, unwanted silencing is of concern. The annual Silent Protest at Rhodes University is a day-long anti-rape protest which draws attention to the attitudes and practices which silence victim-survivors, and enacts solidarity with victim-survivors of sexual violence. I conducted a three year “critically engaged, activist ethnography” with the community of Silent Protestors and organisers. My focus was on the stories that victim-survivors told about the impacts of participating in the protest. Changes in the research context allowed for a participatory action research cycle to be incorporated into the methodology. This offered an opportunity to implement and evaluate some changes suggested by my research contributors. My contributor’s narratives highlighted the ways in which as a society we position those who have been subjected to sexual violence in ways that are limited and limiting and which diminish victim-survivors’ sense of agency and value. This malignant positioning restricts the choices available to victim-survivors. The anticipation of being positioned in negative ways can inhibit victim-survivors from disclosing a violation and accessing care and justice. Participation in the Silent Protest seems to stand against some of the factors which inhibit acknowledgement and disclosure. Through their participation many women took up opportunities to share their story with at least one other person and to receive and offer care. Most described participation as having been beneficial and having helped them move towards living well again. The ways in which the Silent Protest positioned victim-survivors and the choices they were offered in relation to that positioning seemed to open up opportunities for thinking, feeling and acting in ways that they preferred. This suggests that interventions which mobilise both political resistance and mutual care hold promise for developing more accessible and effective services to those affected by sexual violence. , Thesis (PhD) -- Faculty of Humanities, Psychology, 2023
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Date Issued: 2023-03-29

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