A cytotoxic pentadecapeptide from a South African Didemnid tunicate
- Gallegos, D, Serrill, J, Parker-Nance, Shirley, Dorrington, Rosemary A, Ishmael, J, McPhail, Kerry L
- Authors: Gallegos, D , Serrill, J , Parker-Nance, Shirley , Dorrington, Rosemary A , Ishmael, J , McPhail, Kerry L
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/65935 , vital:28863 , https://doi.org/10.1055/s-0036-1596683
- Description: publisher version , The rate of discovery of new natural product chemical entities has plateaued, and unique populations of endemic, biologically diverse sessile marine organisms represent increasingly critical opportunities to discover new chemistry. Discovery of the mandelalides [1] as potent inhibitors of cancer cell growth from the new South African tunicate Lissoclinum mandelai is an example of the diverse suites of metabolites with potent biological activities that have been isolated from tunicates and other filter-feeding sessile marine organisms that house complex microbial consortia. Further investigation of archived and new tunicate collections from Algoa Bay, South Africa, has revealed a group of didemnid tunicates with an unusual gelatinous morphology similar to Lissoclinum mandelai. Using a bioassay-guided isolation approach, a new “gelatinous” species of the genus Didemnum has yielded a cytotoxic pentadecapeptide with a molecular mass of 1603.7688 Da, comprising fifteen residues including both proteinogenic and non-proteinogenic amino acids. The pure compound inhibited both HeLa cervical cancer and NCI-H460 non-small cell lung cancer cell lines when tested at 30 nM in preliminary assays against cells seeded at low densities. Inhibition of cancer cells at low starting density may be indicative of an anti-proliferative mechanism of action. The compound did not show antibacterial activity against Vibrio cholera. Didemnin B and its clinically approved analogue dehydrodidemnin B (plitidepsin, Aplidin®) [2, 3] are important macrocyclic depsipeptides from a didemnid tunicate. The pentadecapeptide reported here provides justification for our continued investigation of unique, endemic didemnid tunicates from South Africa as a source of new macrocyclic natural products with cytotoxic, anti-viral or antimicrobial activity. , We acknowledge the South African government for permission to collect the subject tunicate (Collection Permit No. 278 RES2013/43)
- Full Text: false
- Date Issued: 2016
- Authors: Gallegos, D , Serrill, J , Parker-Nance, Shirley , Dorrington, Rosemary A , Ishmael, J , McPhail, Kerry L
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/65935 , vital:28863 , https://doi.org/10.1055/s-0036-1596683
- Description: publisher version , The rate of discovery of new natural product chemical entities has plateaued, and unique populations of endemic, biologically diverse sessile marine organisms represent increasingly critical opportunities to discover new chemistry. Discovery of the mandelalides [1] as potent inhibitors of cancer cell growth from the new South African tunicate Lissoclinum mandelai is an example of the diverse suites of metabolites with potent biological activities that have been isolated from tunicates and other filter-feeding sessile marine organisms that house complex microbial consortia. Further investigation of archived and new tunicate collections from Algoa Bay, South Africa, has revealed a group of didemnid tunicates with an unusual gelatinous morphology similar to Lissoclinum mandelai. Using a bioassay-guided isolation approach, a new “gelatinous” species of the genus Didemnum has yielded a cytotoxic pentadecapeptide with a molecular mass of 1603.7688 Da, comprising fifteen residues including both proteinogenic and non-proteinogenic amino acids. The pure compound inhibited both HeLa cervical cancer and NCI-H460 non-small cell lung cancer cell lines when tested at 30 nM in preliminary assays against cells seeded at low densities. Inhibition of cancer cells at low starting density may be indicative of an anti-proliferative mechanism of action. The compound did not show antibacterial activity against Vibrio cholera. Didemnin B and its clinically approved analogue dehydrodidemnin B (plitidepsin, Aplidin®) [2, 3] are important macrocyclic depsipeptides from a didemnid tunicate. The pentadecapeptide reported here provides justification for our continued investigation of unique, endemic didemnid tunicates from South Africa as a source of new macrocyclic natural products with cytotoxic, anti-viral or antimicrobial activity. , We acknowledge the South African government for permission to collect the subject tunicate (Collection Permit No. 278 RES2013/43)
- Full Text: false
- Date Issued: 2016
Vertebrate Biostratigraphy of the Witteberg Group and the Devonian-Carboniferous boundary in South Africa
- Authors: Gess, Robert W
- Date: 2016
- Language: English
- Type: text , boook chapter
- Identifier: http://hdl.handle.net/10962/73987 , vital:30248 , https://doi.org/10.1007/978-3-319-40859-0_13
- Description: Witteberg Group rocks are Late Devonian to Early Carboniferous in age. Comparison with Laurasian sea-level curves has correlated the earliest Carboniferous Tournasian transgression, with the argillaceous Kweekvlei Formation, overlying the arenaceous Witpoort Formation. The Devonian/Carboniferous boundary is globally characterised by a Mass Extinction which extinguished the entire grade of placoderm fish and radically reduced sarcopterygian and acanthodian diversity, preluding an Early Carboniferous radiation of actinopterygians. Analysis of Cape Supergroup biostratigraphy reveals that a fauna preservationally dominated by placoderms, sharks, and gyracanthid acanthodians may be traced from the Upper Bokkeveld Group through the Wagondrift Formation (Witteberg Group) to the Witpoort Formation, wherein it displays an increased diversity of placoderms, as well as sarcopterygians. Overlying strata contain no placoderms or sarcopterygians, but present are some relict sharks and acanthodians, and an increasing abundance of actinopterygians. This congruence confirms sea-level curve based age estimates of the Witteberg Group and the position of the Devonian/Carboniferous boundary.
- Full Text: false
- Date Issued: 2016
- Authors: Gess, Robert W
- Date: 2016
- Language: English
- Type: text , boook chapter
- Identifier: http://hdl.handle.net/10962/73987 , vital:30248 , https://doi.org/10.1007/978-3-319-40859-0_13
- Description: Witteberg Group rocks are Late Devonian to Early Carboniferous in age. Comparison with Laurasian sea-level curves has correlated the earliest Carboniferous Tournasian transgression, with the argillaceous Kweekvlei Formation, overlying the arenaceous Witpoort Formation. The Devonian/Carboniferous boundary is globally characterised by a Mass Extinction which extinguished the entire grade of placoderm fish and radically reduced sarcopterygian and acanthodian diversity, preluding an Early Carboniferous radiation of actinopterygians. Analysis of Cape Supergroup biostratigraphy reveals that a fauna preservationally dominated by placoderms, sharks, and gyracanthid acanthodians may be traced from the Upper Bokkeveld Group through the Wagondrift Formation (Witteberg Group) to the Witpoort Formation, wherein it displays an increased diversity of placoderms, as well as sarcopterygians. Overlying strata contain no placoderms or sarcopterygians, but present are some relict sharks and acanthodians, and an increasing abundance of actinopterygians. This congruence confirms sea-level curve based age estimates of the Witteberg Group and the position of the Devonian/Carboniferous boundary.
- Full Text: false
- Date Issued: 2016
Distribution and ecological assessment of heavy metals in irrigation channel sediments in a typical rural area of south China
- Gu, Chiming, Liu, Yi, Li, Zhiguo, Mohamed, Ibrahim, Zhang, Ronghua, Brooks, Margot, Chen, Fang
- Authors: Gu, Chiming , Liu, Yi , Li, Zhiguo , Mohamed, Ibrahim , Zhang, Ronghua , Brooks, Margot , Chen, Fang
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66924 , vital:29000 , ISSN 0925-8574 , https://doi.org/10.1016/j.ecoleng.2016.01.054
- Description: Publisher version , Elevated levels of heavy metals in sediments of irrigation channels can pose risks for crops and livestock, as well as for human health. In this study sediment samples were collected from the irrigation channel in a typical rural area of south China, and digested, in order to analyze their contents for the presence of heavy metals Cu, Zn, Pb, Cr, Cd, and Ni, as well as the non-metal As, to assess total concentrations and pollution levels. The pollution load index and potential ecological risk index of these elements were utilized to assess contamination levels and ecotoxicity. Our results showed that the concentrations of the 7 elements were in the order of Zn > Ni > Cr > Cu > As > Pb > Cd. With the exception of Cr and Pb, concentrations of elements were higher than their background in soil, especially for Cd (1.79 mg kg−1), As (99.61 mg kg−1) and Ni (142.62 mg kg−1), which were 18.49, 8.89 and 5.30 times their background concentrations, respectively. The whole sampled zone was characterized by medium pollution and had a very high potential ecological risk. The area of arable land presented a medium pollution risk, while areas near to the path or road showed high risk of pollution. The predominant contributors to elevated ecological risk for the whole zone were Cd and As.
- Full Text: false
- Date Issued: 2016
- Authors: Gu, Chiming , Liu, Yi , Li, Zhiguo , Mohamed, Ibrahim , Zhang, Ronghua , Brooks, Margot , Chen, Fang
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66924 , vital:29000 , ISSN 0925-8574 , https://doi.org/10.1016/j.ecoleng.2016.01.054
- Description: Publisher version , Elevated levels of heavy metals in sediments of irrigation channels can pose risks for crops and livestock, as well as for human health. In this study sediment samples were collected from the irrigation channel in a typical rural area of south China, and digested, in order to analyze their contents for the presence of heavy metals Cu, Zn, Pb, Cr, Cd, and Ni, as well as the non-metal As, to assess total concentrations and pollution levels. The pollution load index and potential ecological risk index of these elements were utilized to assess contamination levels and ecotoxicity. Our results showed that the concentrations of the 7 elements were in the order of Zn > Ni > Cr > Cu > As > Pb > Cd. With the exception of Cr and Pb, concentrations of elements were higher than their background in soil, especially for Cd (1.79 mg kg−1), As (99.61 mg kg−1) and Ni (142.62 mg kg−1), which were 18.49, 8.89 and 5.30 times their background concentrations, respectively. The whole sampled zone was characterized by medium pollution and had a very high potential ecological risk. The area of arable land presented a medium pollution risk, while areas near to the path or road showed high risk of pollution. The predominant contributors to elevated ecological risk for the whole zone were Cd and As.
- Full Text: false
- Date Issued: 2016
Politics at a distance from the state: radical, South African and Zimbabwean praxis today
- Helliker, Kirk D, van der Walt, Lucien
- Authors: Helliker, Kirk D , van der Walt, Lucien
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/71364 , vital:29837 , https://doi.org/10.1080/02589001.2016.1240792
- Description: For decades, most anti-capitalist and anti-imperialist movements identified radical social transformation with the capture of state power. The collapse of supposedly enabling states led recently to a crisis of left and working class politics. But this has also opened space for the rediscovery of society-centred, anti-capitalist modes of bottom-up change, labelled as ‘at a distance’ politics. These modes have registered important successes in practice, such as the Zapatistas in Mexico, and have involved strands of anarchism and syndicalism, and autonomist Marxism. This article, an introduction to a collection of papers emerging from a 2012 conference of academics and activists in South Africa, aims to help articulate an understanding of social transformation from below that has been analytically and politically side-lined not only in South Africa (and Zimbabwe), but globally. In doing so, it provides a preliminary attempt to map and create a dialogue between three major positions within the broad category of ‘at a distance’ politics.
- Full Text: false
- Date Issued: 2016
- Authors: Helliker, Kirk D , van der Walt, Lucien
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/71364 , vital:29837 , https://doi.org/10.1080/02589001.2016.1240792
- Description: For decades, most anti-capitalist and anti-imperialist movements identified radical social transformation with the capture of state power. The collapse of supposedly enabling states led recently to a crisis of left and working class politics. But this has also opened space for the rediscovery of society-centred, anti-capitalist modes of bottom-up change, labelled as ‘at a distance’ politics. These modes have registered important successes in practice, such as the Zapatistas in Mexico, and have involved strands of anarchism and syndicalism, and autonomist Marxism. This article, an introduction to a collection of papers emerging from a 2012 conference of academics and activists in South Africa, aims to help articulate an understanding of social transformation from below that has been analytically and politically side-lined not only in South Africa (and Zimbabwe), but globally. In doing so, it provides a preliminary attempt to map and create a dialogue between three major positions within the broad category of ‘at a distance’ politics.
- Full Text: false
- Date Issued: 2016
Disaster management policy options to address the sanitation challenges in South Africa
- Hoossein, Shafick, Tandlich, Roman, Whittington-Jones, Kevin J, Laubscher, Richard K, Madikizela, Phindile, Zuma, Bongumusa M
- Authors: Hoossein, Shafick , Tandlich, Roman , Whittington-Jones, Kevin J , Laubscher, Richard K , Madikizela, Phindile , Zuma, Bongumusa M
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/75938 , vital:30485 , https://www.neha.org/node/28591
- Description: The current population of South Africa has been migrating into informal urban settlements that lack adequate sanitation service delivery, caused at least in part by the lack of the necessary skills in the local government sector and the lack of buy in from the community into the provided sanitation facilities. The authors report results of policy research into the relevant disaster management options that could be applied to improve the sanitation service delivery in South Africa. The best policy option was identified as the draft Disaster Management Regulations: Disaster Management. Local government can use these tools through the formation of the volunteer units from the nongovernmental organization sector, the business community, and from among the end users of sanitation facilities. Formation of the volunteer unit should follow the principles of cooperative governance and participatory approach to disaster management. Implementation should be facilitated through the adoption of locally specific municipal by laws.
- Full Text: false
- Date Issued: 2016
- Authors: Hoossein, Shafick , Tandlich, Roman , Whittington-Jones, Kevin J , Laubscher, Richard K , Madikizela, Phindile , Zuma, Bongumusa M
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/75938 , vital:30485 , https://www.neha.org/node/28591
- Description: The current population of South Africa has been migrating into informal urban settlements that lack adequate sanitation service delivery, caused at least in part by the lack of the necessary skills in the local government sector and the lack of buy in from the community into the provided sanitation facilities. The authors report results of policy research into the relevant disaster management options that could be applied to improve the sanitation service delivery in South Africa. The best policy option was identified as the draft Disaster Management Regulations: Disaster Management. Local government can use these tools through the formation of the volunteer units from the nongovernmental organization sector, the business community, and from among the end users of sanitation facilities. Formation of the volunteer unit should follow the principles of cooperative governance and participatory approach to disaster management. Implementation should be facilitated through the adoption of locally specific municipal by laws.
- Full Text: false
- Date Issued: 2016
The impact of geographical origin of two strains of the herbivore, Eccritotarsus catarinensis, on several fitness traits in response to temperature
- Ismail, Mohannad, Brooks, Margot
- Authors: Ismail, Mohannad , Brooks, Margot
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66914 , vital:28999 , ISSN 0306-4565 , https://doi.org/10.1016/j.jtherbio.2016.07.008
- Description: Publisher version , Adaptation to temperature changes is vital to reduce adverse effects on individuals, and some may present phenotypic changes, which might be accompanied with physiological costs in fitness traits. The objective of this study was to determine whether the two strains of the herbivore Eccritotarsus catarinensis, a biological control agent against water hyacinth in South Africa, differ in their responses to temperature according to their geographical origin. We experimentally quantified the responses of the two strains, at three constant temperatures: 20 °C, 25 °C and 30 °C, using laboratory cultures that originated from Brazil and Peru, where climates differ. Reproductive output, egg hatching rate, sex ratio and longevity were recorded at each temperature. Fitness traits for both strains were significantly reduced at 30 °C compared with 25 °C and 20 °C in two successive generations. Nonetheless, Peruvian individuals continued their development at 30 °C, whereas Brazilian individuals that succeeded in emerging did not continue their development. In contrast, sex ratio was unaffected by temperature. The Peruvian strain of E. catarinensis presented different phenotypes depending on temperature and was more adapted to extreme high temperature than the Brazilian strain. The tropical origin of the population induces the insect to tolerate the extreme high temperature. We suggest that the Peruvian strain could be better suited for release to control water hyacinth in nature, particularly in regions where temperature is high.
- Full Text: false
- Date Issued: 2016
- Authors: Ismail, Mohannad , Brooks, Margot
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66914 , vital:28999 , ISSN 0306-4565 , https://doi.org/10.1016/j.jtherbio.2016.07.008
- Description: Publisher version , Adaptation to temperature changes is vital to reduce adverse effects on individuals, and some may present phenotypic changes, which might be accompanied with physiological costs in fitness traits. The objective of this study was to determine whether the two strains of the herbivore Eccritotarsus catarinensis, a biological control agent against water hyacinth in South Africa, differ in their responses to temperature according to their geographical origin. We experimentally quantified the responses of the two strains, at three constant temperatures: 20 °C, 25 °C and 30 °C, using laboratory cultures that originated from Brazil and Peru, where climates differ. Reproductive output, egg hatching rate, sex ratio and longevity were recorded at each temperature. Fitness traits for both strains were significantly reduced at 30 °C compared with 25 °C and 20 °C in two successive generations. Nonetheless, Peruvian individuals continued their development at 30 °C, whereas Brazilian individuals that succeeded in emerging did not continue their development. In contrast, sex ratio was unaffected by temperature. The Peruvian strain of E. catarinensis presented different phenotypes depending on temperature and was more adapted to extreme high temperature than the Brazilian strain. The tropical origin of the population induces the insect to tolerate the extreme high temperature. We suggest that the Peruvian strain could be better suited for release to control water hyacinth in nature, particularly in regions where temperature is high.
- Full Text: false
- Date Issued: 2016
Expanding the host range of small insect RNA viruses: Providence virus (Carmotetraviridae) infects and replicates in a human tissue culture cell line
- Jiwaji, Meesbah, Short, James R, Dorrington, Rosemary A
- Authors: Jiwaji, Meesbah , Short, James R , Dorrington, Rosemary A
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/65979 , vital:28874 , https://doi.org/10.1099/jgv.0.000578
- Description: publisher version , Tetraviruses are small, positive (+ve)-sense ssRNA viruses that infect the midgut cells of lepidopteran larvae. Providence virus(PrV) is the only member of the family Carmotetraviridae (previously Tetraviridae). PrV particles exhibit the characteristic tetraviral T=4 icosahedral symmetry, but PrV is distinct from other tetraviruses with respect to genome organization and viral non-structural proteins. Currently, PrV is the only tetravirus known to infect and replicate in lepidopteran cell culture lines. In this report we demonstrate, using immunofluorescence microscopy, that PrV infects and replicates in a human tissue culture cell line (HeLa), producing infectious virus particles. We also provide evidence for PrV replication in vitro in insect, mammalian and plant cell-free systems. This study challenges the long-held view that tetraviruses have a narrow host range confined to one or a few lepidopteran species and highlights the need to consider the potential for apparently non-infectious viruses to be transferred to new hosts in the laboratory.
- Full Text: false
- Date Issued: 2016
- Authors: Jiwaji, Meesbah , Short, James R , Dorrington, Rosemary A
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/65979 , vital:28874 , https://doi.org/10.1099/jgv.0.000578
- Description: publisher version , Tetraviruses are small, positive (+ve)-sense ssRNA viruses that infect the midgut cells of lepidopteran larvae. Providence virus(PrV) is the only member of the family Carmotetraviridae (previously Tetraviridae). PrV particles exhibit the characteristic tetraviral T=4 icosahedral symmetry, but PrV is distinct from other tetraviruses with respect to genome organization and viral non-structural proteins. Currently, PrV is the only tetravirus known to infect and replicate in lepidopteran cell culture lines. In this report we demonstrate, using immunofluorescence microscopy, that PrV infects and replicates in a human tissue culture cell line (HeLa), producing infectious virus particles. We also provide evidence for PrV replication in vitro in insect, mammalian and plant cell-free systems. This study challenges the long-held view that tetraviruses have a narrow host range confined to one or a few lepidopteran species and highlights the need to consider the potential for apparently non-infectious viruses to be transferred to new hosts in the laboratory.
- Full Text: false
- Date Issued: 2016
A case study of the opportunities and trade-offs associated with deproclamation of a protected area following a land claim in South Africa
- Krüger, Ruth, Cundill, Georgina, Thondhlana, Gladman
- Authors: Krüger, Ruth , Cundill, Georgina , Thondhlana, Gladman
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/67791 , vital:29145 , https://doi.org/10.1080/13549839.2015.1065804
- Description: Publisher version , Reconciling conservation and social justice imperatives is a major challenge facing many postcolonial states worldwide. Where historically disenfranchised communities have laid legal claim to protected areas, the typical resolution has been collaborative management agreements between the state and claimant communities. The real outcomes of such strategies for people and ecosystems have been seriously questioned, although alternative approaches are seldom explored. Here, we reflect on one such alternative that was pursued in a case in South Africa, where the land was handed back to the community and a replacement protected area created. Our objective was to explore the opportunities and trade-offs associated with this approach for communities and conservation agencies alike, and to compare these to typical collaborative management outcomes. Methods included key informant interviews, focus group discussions and household surveys. We find that, surprisingly, this approach created more benefits for the conservation agency than for claimant communities. Indeed, the community experiences bore a striking resemblance to those experienced in collaborative management settings: intra-community conflict, confusion over leadership and serious questions about the boundaries of the “community”. Processes aimed at redressing past injustice in disputes over conservation land, regardless of the approach adopted, must bring with them a strong commitment to building institutional and leadership capacities within communities, and pay serious attention to the ways in which equity and social justice can be fostered after the settlement of a land claim. Settlement agreements are frequently treated as the final step towards social justice, but are in fact just the beginning.
- Full Text: false
- Date Issued: 2016
- Authors: Krüger, Ruth , Cundill, Georgina , Thondhlana, Gladman
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/67791 , vital:29145 , https://doi.org/10.1080/13549839.2015.1065804
- Description: Publisher version , Reconciling conservation and social justice imperatives is a major challenge facing many postcolonial states worldwide. Where historically disenfranchised communities have laid legal claim to protected areas, the typical resolution has been collaborative management agreements between the state and claimant communities. The real outcomes of such strategies for people and ecosystems have been seriously questioned, although alternative approaches are seldom explored. Here, we reflect on one such alternative that was pursued in a case in South Africa, where the land was handed back to the community and a replacement protected area created. Our objective was to explore the opportunities and trade-offs associated with this approach for communities and conservation agencies alike, and to compare these to typical collaborative management outcomes. Methods included key informant interviews, focus group discussions and household surveys. We find that, surprisingly, this approach created more benefits for the conservation agency than for claimant communities. Indeed, the community experiences bore a striking resemblance to those experienced in collaborative management settings: intra-community conflict, confusion over leadership and serious questions about the boundaries of the “community”. Processes aimed at redressing past injustice in disputes over conservation land, regardless of the approach adopted, must bring with them a strong commitment to building institutional and leadership capacities within communities, and pay serious attention to the ways in which equity and social justice can be fostered after the settlement of a land claim. Settlement agreements are frequently treated as the final step towards social justice, but are in fact just the beginning.
- Full Text: false
- Date Issued: 2016
Revisiting cellulase production and redefining current strategies based on major challenges
- Kuhad, Ramesh Chander, Deswal, Deepa, Sharma, Sonia, Bhattacharya, Abhishek, Jain, Kavish Kumar, Kaur, Amandeep, Pletschke, Brett I, Singh, Ajay, Karp, Matti
- Authors: Kuhad, Ramesh Chander , Deswal, Deepa , Sharma, Sonia , Bhattacharya, Abhishek , Jain, Kavish Kumar , Kaur, Amandeep , Pletschke, Brett I , Singh, Ajay , Karp, Matti
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66142 , vital:28909 , https://doi.org/10.1016/j.rser.2015.10.132
- Description: publisher version , Lignocellulosic biomass has been considered as an important and sustainable source of renewable energy. Cellulose constitutes the major component of the lignocellulosic biomass and also offers maximum recalcitrance towards its fullest utilization. The enzymatic breakdown of cellulose is achieved through cellulases. Diverse forms of microbes including fungi, bacteria, actinomycetes and yeast are known to produce cellulases that have found extensive application in various industries. Due to the current global political unrest over oil prices and the threat of global warming following combustion of fossil fuels, the paradigm of research is now focused on biofuel production from plant biomass. Conventional approaches have not been economically feasible for meeting the demands of the industry. This review provides an update regarding the status of present microbial cellulase production technologies and research with special reference to solid state fermentation and different molecular techniques such as mutagenesis, metabolic engineering and heterologous gene expression of cellulases from different microbial domains with improved catalytic and stability properties. Metagenomic and genomic studies for mining of novel cellulase genes in addition to screening of culturable strains using conventional methods have been advanced. In addition the bottlenecks associated with cellulase production and how the future research needs to be directed to provide a comprehensive technology for the production of cellulases with novel traits for application at an industrial level without economic constraints are discussed.
- Full Text: false
- Date Issued: 2016
- Authors: Kuhad, Ramesh Chander , Deswal, Deepa , Sharma, Sonia , Bhattacharya, Abhishek , Jain, Kavish Kumar , Kaur, Amandeep , Pletschke, Brett I , Singh, Ajay , Karp, Matti
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66142 , vital:28909 , https://doi.org/10.1016/j.rser.2015.10.132
- Description: publisher version , Lignocellulosic biomass has been considered as an important and sustainable source of renewable energy. Cellulose constitutes the major component of the lignocellulosic biomass and also offers maximum recalcitrance towards its fullest utilization. The enzymatic breakdown of cellulose is achieved through cellulases. Diverse forms of microbes including fungi, bacteria, actinomycetes and yeast are known to produce cellulases that have found extensive application in various industries. Due to the current global political unrest over oil prices and the threat of global warming following combustion of fossil fuels, the paradigm of research is now focused on biofuel production from plant biomass. Conventional approaches have not been economically feasible for meeting the demands of the industry. This review provides an update regarding the status of present microbial cellulase production technologies and research with special reference to solid state fermentation and different molecular techniques such as mutagenesis, metabolic engineering and heterologous gene expression of cellulases from different microbial domains with improved catalytic and stability properties. Metagenomic and genomic studies for mining of novel cellulase genes in addition to screening of culturable strains using conventional methods have been advanced. In addition the bottlenecks associated with cellulase production and how the future research needs to be directed to provide a comprehensive technology for the production of cellulases with novel traits for application at an industrial level without economic constraints are discussed.
- Full Text: false
- Date Issued: 2016
Partnerships and parents–relationships in tutorial programmes
- Layton, Delia M, McKenna, Sioux
- Authors: Layton, Delia M , McKenna, Sioux
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66699 , vital:28983 , ISSN 1469-8366 , https://doi.org/10.1080/07294360.2015.1087471
- Description: The tutorial system is considered to be a useful pedagogical intervention to improve student retention, particularly in the context of a first-year student’s experience of entering university. For these novice students to achieve academic success, it is important that they are given access to the subject-specific knowledge and practices in their different disciplines, that is, that they acquire ‘epistemological access’. A recent study of the tutorial system in a South African university (Layton, D.M. [2013]. A social realist account of the tutorial system at the University of Johannesburg (Unpublished doctoral dissertation). Rhodes University, Grahamstown), sought to discover to what extent tutorials were discursively constructed as being about the enablement of epistemological access. This paper focuses on two discourses that emerged from the study – the parent discourse and the partnership discourse. Both discourses were concerned with relationships between key stakeholders in the tutorial programme. Given that tutorials are considered to be spaces in which more intimate learning can take place than in the anonymous environment of the large lecture hall, an interrogation of the relationships fostered in tutorials is important. The parent discourse, in which students were positioned as ‘kids’ needing care, was supportive of new students but ran the risk of being patronising and reductionist. The partnerships discourse, in which tutors and academics were seen to be working together towards the common goal of student success, was seen to be enabling of epistemological access. But it required a commitment to teaching endeavours that was in tension with the institutional focus on research. Through a social realist analysis of the two discourses constructing relationships in the tutorial system, we conclude that these discourses have the power to both constrain and enable the extent to which the tutorial system can be a site of epistemological access.
- Full Text: false
- Date Issued: 2016
- Authors: Layton, Delia M , McKenna, Sioux
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66699 , vital:28983 , ISSN 1469-8366 , https://doi.org/10.1080/07294360.2015.1087471
- Description: The tutorial system is considered to be a useful pedagogical intervention to improve student retention, particularly in the context of a first-year student’s experience of entering university. For these novice students to achieve academic success, it is important that they are given access to the subject-specific knowledge and practices in their different disciplines, that is, that they acquire ‘epistemological access’. A recent study of the tutorial system in a South African university (Layton, D.M. [2013]. A social realist account of the tutorial system at the University of Johannesburg (Unpublished doctoral dissertation). Rhodes University, Grahamstown), sought to discover to what extent tutorials were discursively constructed as being about the enablement of epistemological access. This paper focuses on two discourses that emerged from the study – the parent discourse and the partnership discourse. Both discourses were concerned with relationships between key stakeholders in the tutorial programme. Given that tutorials are considered to be spaces in which more intimate learning can take place than in the anonymous environment of the large lecture hall, an interrogation of the relationships fostered in tutorials is important. The parent discourse, in which students were positioned as ‘kids’ needing care, was supportive of new students but ran the risk of being patronising and reductionist. The partnerships discourse, in which tutors and academics were seen to be working together towards the common goal of student success, was seen to be enabling of epistemological access. But it required a commitment to teaching endeavours that was in tension with the institutional focus on research. Through a social realist analysis of the two discourses constructing relationships in the tutorial system, we conclude that these discourses have the power to both constrain and enable the extent to which the tutorial system can be a site of epistemological access.
- Full Text: false
- Date Issued: 2016
Changing practices, changing values?: a Bernsteinian analysis of knowledge production and knowledge exchange in two UK universities
- Little, Brenda, Abbas, Andrea, Singh, Mala
- Authors: Little, Brenda , Abbas, Andrea , Singh, Mala
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66934 , vital:29002 , https://doi.org/10.1007/978-94-017-7369-0_8
- Description: publisher version , Bernstein’s concept of classification and framing links notions of knowledge, democracy and social justice, providing a perspective from which to address critical questions of what knowledge is produced, who has access to it, and how knowledge is distributed. Bernstein’s conceptual framework is used to inform an analysis of national policies steering knowledge production and knowledge transfer in the UK, and the changing practices and values associated with knowledge production and knowledge transfer in two UK institutional case study universities. The analysis reveals how reputational and financial consequences of the formal assessment of research quality interacts with the institutional and disciplinary contexts of research units to differently shape what knowledge is valued and produced, and with whom it is shared. Five discursive areas, each involving a complex set of classifications (power) and framings (control) are identified, namely: the national research assessment framework; the economic value of research; discourses of social and academic values; academic freedoms; and mixed-discipline research and the interdisciplinary nature of real world problems. Though competing and sometimes contradictory values seem to underlie academics’ knowledge work, it seems that the strong framing for knowledge production and knowledge exchange provided by national policies steers staff efforts towards economised codes of knowledge. The conclusion suggests that such a strong steer does not value social transformation in all its diverse non-economistic dimensions and limits universities’ potential to transform societies to further social justice.
- Full Text: false
- Date Issued: 2016
- Authors: Little, Brenda , Abbas, Andrea , Singh, Mala
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66934 , vital:29002 , https://doi.org/10.1007/978-94-017-7369-0_8
- Description: publisher version , Bernstein’s concept of classification and framing links notions of knowledge, democracy and social justice, providing a perspective from which to address critical questions of what knowledge is produced, who has access to it, and how knowledge is distributed. Bernstein’s conceptual framework is used to inform an analysis of national policies steering knowledge production and knowledge transfer in the UK, and the changing practices and values associated with knowledge production and knowledge transfer in two UK institutional case study universities. The analysis reveals how reputational and financial consequences of the formal assessment of research quality interacts with the institutional and disciplinary contexts of research units to differently shape what knowledge is valued and produced, and with whom it is shared. Five discursive areas, each involving a complex set of classifications (power) and framings (control) are identified, namely: the national research assessment framework; the economic value of research; discourses of social and academic values; academic freedoms; and mixed-discipline research and the interdisciplinary nature of real world problems. Though competing and sometimes contradictory values seem to underlie academics’ knowledge work, it seems that the strong framing for knowledge production and knowledge exchange provided by national policies steers staff efforts towards economised codes of knowledge. The conclusion suggests that such a strong steer does not value social transformation in all its diverse non-economistic dimensions and limits universities’ potential to transform societies to further social justice.
- Full Text: false
- Date Issued: 2016
Learning style of Chinese event management students
- Louw, Mattheus J, Louw, Lynette, Li, Yanxia
- Authors: Louw, Mattheus J , Louw, Lynette , Li, Yanxia
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/69167 , vital:29438 , https://doi.org/10.1515/9783110366778-028
- Description: There is a demand for social development in China by establishing, inter alia, a framework focusing on the employability of university graduates and developing self-directed learners. The key to achieving this would be to gain a better understanding of how learning styles, as one of the cognitive factors, contribute towards academic performance in order to provide meaningful learning experiences.
- Full Text: false
- Date Issued: 2016
- Authors: Louw, Mattheus J , Louw, Lynette , Li, Yanxia
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/69167 , vital:29438 , https://doi.org/10.1515/9783110366778-028
- Description: There is a demand for social development in China by establishing, inter alia, a framework focusing on the employability of university graduates and developing self-directed learners. The key to achieving this would be to gain a better understanding of how learning styles, as one of the cognitive factors, contribute towards academic performance in order to provide meaningful learning experiences.
- Full Text: false
- Date Issued: 2016
The inhibitory effects of various substrate pre-treatment by-products and wash liquors on mannanolytic enzymes
- Malgas, Samkelo, Van Dyk, J Susan, Abboo, Sagaran, Pletschke, Brett I
- Authors: Malgas, Samkelo , Van Dyk, J Susan , Abboo, Sagaran , Pletschke, Brett I
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66156 , vital:28911 , https://doi.org/10.1016/j.molcatb.2015.11.014
- Description: publisher version , Biomass pre-treatment is essential for achieving high levels of bioconversion through increased accessibility of hydrolytic enzymes to hydrolysable carbohydrates. However, pre-treatment by-products, such as sugar and lignin degradation products, can negatively affect the performance of hydrolytic (mannanolytic) enzymes. In this study, two monomeric sugars, five sugar degradation products, five lignin derivatives and four liquors from biomass feedstocks pre-treated by different technologies, were evaluated for their inhibitory effects on mannanolytic enzymes (α-galactosidases, β-mannanases and β-mannosidases). Lignin derivatives elicited the greatest inhibitory effect on the mannanolytic enzymes, followed by organic acids and furan derivatives derived from sugar degradation. Lignin derivative inhibition appeared to be as a result of protein–phenolic complexation, leading to protein precipitating out of solution. The functional groups on the phenolic lignin derivatives appeared to be directly related to the ability of the phenolic to interfere with enzyme activity, with the phenolic containing the highest hydroxyl group content exhibiting the greatest inhibition. It was also demonstrated that various pre-treatment technologies render different pre-treatment soluble by-products which interact in various ways with the mannanolytic enzymes. The different types of biomass (i.e. different plant species) were also shown to release different by-products that interacted with the mannanolytic enzymes in a diverse manner even when the biomass was pre-treated using the same technology. Enzyme inhibition by pre-treatment by-products can be alleviated through the removal of these compounds prior to enzymatic hydrolysis to maximize enzyme activity.
- Full Text: false
- Date Issued: 2016
- Authors: Malgas, Samkelo , Van Dyk, J Susan , Abboo, Sagaran , Pletschke, Brett I
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66156 , vital:28911 , https://doi.org/10.1016/j.molcatb.2015.11.014
- Description: publisher version , Biomass pre-treatment is essential for achieving high levels of bioconversion through increased accessibility of hydrolytic enzymes to hydrolysable carbohydrates. However, pre-treatment by-products, such as sugar and lignin degradation products, can negatively affect the performance of hydrolytic (mannanolytic) enzymes. In this study, two monomeric sugars, five sugar degradation products, five lignin derivatives and four liquors from biomass feedstocks pre-treated by different technologies, were evaluated for their inhibitory effects on mannanolytic enzymes (α-galactosidases, β-mannanases and β-mannosidases). Lignin derivatives elicited the greatest inhibitory effect on the mannanolytic enzymes, followed by organic acids and furan derivatives derived from sugar degradation. Lignin derivative inhibition appeared to be as a result of protein–phenolic complexation, leading to protein precipitating out of solution. The functional groups on the phenolic lignin derivatives appeared to be directly related to the ability of the phenolic to interfere with enzyme activity, with the phenolic containing the highest hydroxyl group content exhibiting the greatest inhibition. It was also demonstrated that various pre-treatment technologies render different pre-treatment soluble by-products which interact in various ways with the mannanolytic enzymes. The different types of biomass (i.e. different plant species) were also shown to release different by-products that interacted with the mannanolytic enzymes in a diverse manner even when the biomass was pre-treated using the same technology. Enzyme inhibition by pre-treatment by-products can be alleviated through the removal of these compounds prior to enzymatic hydrolysis to maximize enzyme activity.
- Full Text: false
- Date Issued: 2016
LCT in mixed-methods research: evolving an instrument for quantitative data
- Maton, Karl, Howard, Sarah Katherine
- Authors: Maton, Karl , Howard, Sarah Katherine
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66479 , vital:28954
- Description: publisher version , A mantra of social science declares a fundamental divide between the quantitative and the qualitative that involves more than methods. According to this depiction, the two methodologies are intrinsically associated with a range of ontological, epistemological, political and moral stances. Each of these constellations of stances is strongly integrated, such that choice of method is held to involve a series of associated choices. Each constellation is also strongly opposed to the other, along axes labelled positivism/constructivism, scientism/humanism, conservative/critical, old/new, among others. These ‘binary constellations’ (Maton 2014b: 148-70) offer a forced choice between two tightly-knit sets of practices that are portrayed as jointly exhaustive and mutually exclusive. So widespread is this methodological binarism that many scholars ‘are left with the impression that they have to pledge allegiance to one research school of thought or the other’ (Johnson and Onwuegbuzie 2004: 14). A competing mantra disclaims this divide. Distinctions underpinning the picture of binary constellations have been regularly dissolved. Arguments that one deals with numbers, the other with words, one studies behaviour, the other reveals meanings, one is hypothetico-deductive, the other inductive, one enables generalization, the other explores singular depth, among others, have been repeatedly undermined (e.g. Hammersley 1992). Indeed, the death of the divide is frequently declared. Calls for ‘transcending’ (Salomon 1991) or ‘getting over’ (Howe 1992) the quantitative-qualitative debate and arguments for mixed-methods research (Brannen 2005; Johnson and Onwuegbuzie 2004) are recurrent. These calls highlight how the methodologies offer complementary insights for research and demonstrate that eschewing either methodology on principle is unnecessarily renouncing potential explanatory power. However, the call to mixed-methods research remains more breached than honoured. Methodological monotheism remains dominant – studies of education and society typically adopt either quantitative or qualitative methods. As we shall discuss, the former is typically associated with the influence of psychology and the latter is often claimed as emblematic of sociology. Studies utilizing the sociological frameworks on which Legitimation Code Theory (LCT) builds have echoed this pattern by overwhelmingly adopting qualitative methods. Accordingly, Part I of this volume begins by exploring how LCT concepts can be enacted in qualitative research (Chapter 2). However, LCT is not limited to one methodology and a growing body of mixed-methods research is engaging with both qualitative and quantitative data. In this chapter we illustrate how this research works and the gains it offers. For resolutely qualitative researchers, the prospect of reading anything quantitative, even in mixed-methods research, may be unenticing. However, it would be a mistake to pass over this chapter, for several reasons. First, we offer insights into research practice that might surprise such scholars. As Bourdieu argued, ‘methodological indictments are too often no more than a disguised way of making a virtue out of necessity, of feigning to dismiss, to ignore in an active way, what one is ignorant of in fact’ (Bourdieu and Wacquant 1992: 226). Our aim is to contribute towards removing this reason for one-sidedness. We show, for example, how quantitative methods confound their common portrayal as neat, straightforward and procedural; they are complex and involved and require craft work and judgement. Our focus is, therefore, more practical than metaphysical. We shall not enter seemingly endless debates over whether the ‘quantitative-qualitative divide’ refers to paradigms, epistemologies or methods and whether these are complementary or incommensurable. Rather, we discuss the development of an instrument for enacting LCT concepts in quantitative methods and ground this account in real examples of mixed-methods research. Specifically, we trace the evolution of an instrument for embedding specialization codes within questionnaires through its creation for research into school music and then its development within studies of educational technology. Given that mathematics can be off-putting to the noviciate, we minimize discussion of statistics and explain measures in lay terms. Second, this is much more than a story of quantitative methods. The evolution of the instrument both shaped qualitative methods and was shaped by the data they generated, offering insights into how qualitative research can more fully engage with LCT. Its development also involved intimate dialogue with theory that shed fresh light on LCT itself, making explicit the ‘gaze’ embodied by the framework (Chapter 1, this volume). We shall highlight wider lessons learned about the craft of enacting LCT in research, lessons of direct relevance for studies using any methods. Third, we shall illustrate the explanatory power offered by using quantitative and qualitative methods together, such as providing a robust basis for detailed findings, identifying wider-scale trends typically inaccessible to qualitative methods that provide a context for their data, and facilitating knowledge-building through greater replicability across contexts and over time. For example, the technology studies built directly on the music studies to cumulatively develop the instrument and generated probably the largest data set in code sociology: 97,386 responses (83,937 student and 13,449 staff surveys) on the organizing principles of academic subjects, alongside 20 in-depth qualitative case studies of secondary schools. This offers a foundation of substantial breadth and depth for making claims about knowledge practices across the disciplinary map and a firm basis on which future research into disciplinary differences can build. Moreover, the quantitative instrument itself can be adopted or adapted in new studies, further enabling cumulative knowledge-building. Given these substantive, methodological and theoretical gains, it is perhaps surprising there exists any temptation to skip past discussion of mixed-methods research. This reflects the methodological character of the fields in which LCT emerged. We thus begin by briefly illustrating how the sociological frameworks on which the theory builds have become distanced from quantitative methods.
- Full Text: false
- Date Issued: 2016
- Authors: Maton, Karl , Howard, Sarah Katherine
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66479 , vital:28954
- Description: publisher version , A mantra of social science declares a fundamental divide between the quantitative and the qualitative that involves more than methods. According to this depiction, the two methodologies are intrinsically associated with a range of ontological, epistemological, political and moral stances. Each of these constellations of stances is strongly integrated, such that choice of method is held to involve a series of associated choices. Each constellation is also strongly opposed to the other, along axes labelled positivism/constructivism, scientism/humanism, conservative/critical, old/new, among others. These ‘binary constellations’ (Maton 2014b: 148-70) offer a forced choice between two tightly-knit sets of practices that are portrayed as jointly exhaustive and mutually exclusive. So widespread is this methodological binarism that many scholars ‘are left with the impression that they have to pledge allegiance to one research school of thought or the other’ (Johnson and Onwuegbuzie 2004: 14). A competing mantra disclaims this divide. Distinctions underpinning the picture of binary constellations have been regularly dissolved. Arguments that one deals with numbers, the other with words, one studies behaviour, the other reveals meanings, one is hypothetico-deductive, the other inductive, one enables generalization, the other explores singular depth, among others, have been repeatedly undermined (e.g. Hammersley 1992). Indeed, the death of the divide is frequently declared. Calls for ‘transcending’ (Salomon 1991) or ‘getting over’ (Howe 1992) the quantitative-qualitative debate and arguments for mixed-methods research (Brannen 2005; Johnson and Onwuegbuzie 2004) are recurrent. These calls highlight how the methodologies offer complementary insights for research and demonstrate that eschewing either methodology on principle is unnecessarily renouncing potential explanatory power. However, the call to mixed-methods research remains more breached than honoured. Methodological monotheism remains dominant – studies of education and society typically adopt either quantitative or qualitative methods. As we shall discuss, the former is typically associated with the influence of psychology and the latter is often claimed as emblematic of sociology. Studies utilizing the sociological frameworks on which Legitimation Code Theory (LCT) builds have echoed this pattern by overwhelmingly adopting qualitative methods. Accordingly, Part I of this volume begins by exploring how LCT concepts can be enacted in qualitative research (Chapter 2). However, LCT is not limited to one methodology and a growing body of mixed-methods research is engaging with both qualitative and quantitative data. In this chapter we illustrate how this research works and the gains it offers. For resolutely qualitative researchers, the prospect of reading anything quantitative, even in mixed-methods research, may be unenticing. However, it would be a mistake to pass over this chapter, for several reasons. First, we offer insights into research practice that might surprise such scholars. As Bourdieu argued, ‘methodological indictments are too often no more than a disguised way of making a virtue out of necessity, of feigning to dismiss, to ignore in an active way, what one is ignorant of in fact’ (Bourdieu and Wacquant 1992: 226). Our aim is to contribute towards removing this reason for one-sidedness. We show, for example, how quantitative methods confound their common portrayal as neat, straightforward and procedural; they are complex and involved and require craft work and judgement. Our focus is, therefore, more practical than metaphysical. We shall not enter seemingly endless debates over whether the ‘quantitative-qualitative divide’ refers to paradigms, epistemologies or methods and whether these are complementary or incommensurable. Rather, we discuss the development of an instrument for enacting LCT concepts in quantitative methods and ground this account in real examples of mixed-methods research. Specifically, we trace the evolution of an instrument for embedding specialization codes within questionnaires through its creation for research into school music and then its development within studies of educational technology. Given that mathematics can be off-putting to the noviciate, we minimize discussion of statistics and explain measures in lay terms. Second, this is much more than a story of quantitative methods. The evolution of the instrument both shaped qualitative methods and was shaped by the data they generated, offering insights into how qualitative research can more fully engage with LCT. Its development also involved intimate dialogue with theory that shed fresh light on LCT itself, making explicit the ‘gaze’ embodied by the framework (Chapter 1, this volume). We shall highlight wider lessons learned about the craft of enacting LCT in research, lessons of direct relevance for studies using any methods. Third, we shall illustrate the explanatory power offered by using quantitative and qualitative methods together, such as providing a robust basis for detailed findings, identifying wider-scale trends typically inaccessible to qualitative methods that provide a context for their data, and facilitating knowledge-building through greater replicability across contexts and over time. For example, the technology studies built directly on the music studies to cumulatively develop the instrument and generated probably the largest data set in code sociology: 97,386 responses (83,937 student and 13,449 staff surveys) on the organizing principles of academic subjects, alongside 20 in-depth qualitative case studies of secondary schools. This offers a foundation of substantial breadth and depth for making claims about knowledge practices across the disciplinary map and a firm basis on which future research into disciplinary differences can build. Moreover, the quantitative instrument itself can be adopted or adapted in new studies, further enabling cumulative knowledge-building. Given these substantive, methodological and theoretical gains, it is perhaps surprising there exists any temptation to skip past discussion of mixed-methods research. This reflects the methodological character of the fields in which LCT emerged. We thus begin by briefly illustrating how the sociological frameworks on which the theory builds have become distanced from quantitative methods.
- Full Text: false
- Date Issued: 2016
LCT and systemic functional linguistics: Enacting complimentary theories for explanatory power
- Maton, Karl, Martin, James R, Matruglio, Erika S
- Authors: Maton, Karl , Martin, James R , Matruglio, Erika S
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66491 , vital:28955
- Description: publisher version , Interdisciplinarity is the future. Such is the thrust of pronouncements repeatedly heard across the social sciences and humanities. Interdisciplinarity is often equated with intellectually and socially progressive stances and greater responsiveness to business and workplace needs. Yet such axiological and economic benefits are more often assumed or proclaimed than evidenced or demonstrated (Moore 2011). Moreover,what is declared to be 'interdisciplinary' often comprises the appropriation by literary or philosophical discourses of ideas from other fields rather than genuinely interdisciplinary dialogue. Nonetheless,to highlight the vacuity of much written in its name is not to dismiss the potential of interdisciplinarity itself. There are serious ontological and epistemological arguments for bringing disciplines together in substantive research (Bhaskar and Danermark 2006). Simply put,the social world comprises more than the phenomena addressed by any one discipline. Education,for example,involves at least knowledges, knowers, knowing, and the known, implicating insights from, among others,sociology,linguistics,psychology,and philosophy (Maton 2014b: 212-13). This is not to suggest a single study must encompass the disciplinary map in order to recreate reality in its entirety, Rather,it highlights that drawing on more than one disciplinary approach may offer greater explanatory power when exploring a specific problem-situation.
- Full Text: false
- Date Issued: 2016
- Authors: Maton, Karl , Martin, James R , Matruglio, Erika S
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66491 , vital:28955
- Description: publisher version , Interdisciplinarity is the future. Such is the thrust of pronouncements repeatedly heard across the social sciences and humanities. Interdisciplinarity is often equated with intellectually and socially progressive stances and greater responsiveness to business and workplace needs. Yet such axiological and economic benefits are more often assumed or proclaimed than evidenced or demonstrated (Moore 2011). Moreover,what is declared to be 'interdisciplinary' often comprises the appropriation by literary or philosophical discourses of ideas from other fields rather than genuinely interdisciplinary dialogue. Nonetheless,to highlight the vacuity of much written in its name is not to dismiss the potential of interdisciplinarity itself. There are serious ontological and epistemological arguments for bringing disciplines together in substantive research (Bhaskar and Danermark 2006). Simply put,the social world comprises more than the phenomena addressed by any one discipline. Education,for example,involves at least knowledges, knowers, knowing, and the known, implicating insights from, among others,sociology,linguistics,psychology,and philosophy (Maton 2014b: 212-13). This is not to suggest a single study must encompass the disciplinary map in order to recreate reality in its entirety, Rather,it highlights that drawing on more than one disciplinary approach may offer greater explanatory power when exploring a specific problem-situation.
- Full Text: false
- Date Issued: 2016
HOP expression is regulated by p53 and RAS and characteristic of a cancer gene signature
- Mattison, Stacey A, Blatch, Gregory L, Edkins, Adrienne L
- Authors: Mattison, Stacey A , Blatch, Gregory L , Edkins, Adrienne L
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66278 , vital:28928 , https://doi.org/10.1007/s12192-016-0755-8
- Description: publisher version , The Hsp70/Hsp90 organising protein (HOP) is a co-chaperone essential for client protein transfer from Hsp70 to Hsp90 within the Hsp90 chaperone machine. Although HOP is upregulated in various cancers, there is limited information from in vitro studies on how HOP expression is regulated in cancer. The main objective of this study was to identify the HOP promoter and investigate its activity in cancerous cells. Bioinformatic analysis of the -2500 to +16 bp region of the HOP gene identified a large CpG island and a range of putative cis-elements. Many of the cis-elements were potentially bound by transcription factors which are activated by oncogenic pathways. Luciferase reporter assays demonstrated that the upstream region of the HOP gene contains an active promoter in vitro. Truncation of this region suggested that the core HOP promoter region was -855 to +16 bp. HOP promoter activity was highest in Hs578T, HEK293T and SV40- transformed MEF1 cell lines which expressed mutant or inactive p53. In a mutant p53 background, expression of wild-type p53 led to a reduction in promoter activity, while inhibition of wild-type p53 in HeLa cells increased HOP promoter activity. Additionally, in Hs578T and HEK293T cell lines containing inactive p53, expression of HRAS increased HOP promoter activity. However, HRAS activation of the HOP promoter was inhibited by p53 overexpression. These findings suggest for the first time that HOP expression in cancer may be regulated by both RAS activation and p53 inhibition. Taken together, these data suggest that HOP may be part of the cancer gene signature induced by a combination of mutant p53 and mutated RAS that is associated with cellular transformation.
- Full Text: false
- Date Issued: 2018
- Authors: Mattison, Stacey A , Blatch, Gregory L , Edkins, Adrienne L
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66278 , vital:28928 , https://doi.org/10.1007/s12192-016-0755-8
- Description: publisher version , The Hsp70/Hsp90 organising protein (HOP) is a co-chaperone essential for client protein transfer from Hsp70 to Hsp90 within the Hsp90 chaperone machine. Although HOP is upregulated in various cancers, there is limited information from in vitro studies on how HOP expression is regulated in cancer. The main objective of this study was to identify the HOP promoter and investigate its activity in cancerous cells. Bioinformatic analysis of the -2500 to +16 bp region of the HOP gene identified a large CpG island and a range of putative cis-elements. Many of the cis-elements were potentially bound by transcription factors which are activated by oncogenic pathways. Luciferase reporter assays demonstrated that the upstream region of the HOP gene contains an active promoter in vitro. Truncation of this region suggested that the core HOP promoter region was -855 to +16 bp. HOP promoter activity was highest in Hs578T, HEK293T and SV40- transformed MEF1 cell lines which expressed mutant or inactive p53. In a mutant p53 background, expression of wild-type p53 led to a reduction in promoter activity, while inhibition of wild-type p53 in HeLa cells increased HOP promoter activity. Additionally, in Hs578T and HEK293T cell lines containing inactive p53, expression of HRAS increased HOP promoter activity. However, HRAS activation of the HOP promoter was inhibited by p53 overexpression. These findings suggest for the first time that HOP expression in cancer may be regulated by both RAS activation and p53 inhibition. Taken together, these data suggest that HOP may be part of the cancer gene signature induced by a combination of mutant p53 and mutated RAS that is associated with cellular transformation.
- Full Text: false
- Date Issued: 2018
Heat shock protein inhibitors: success stories
- McAlpine, Shelli R, Edkins, Adrienne L
- Authors: McAlpine, Shelli R , Edkins, Adrienne L
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66359 , vital:28940 , https://doi.org/10.1007/978-3-319-32607-8
- Description: publisher version , Introduction: Medicinal chemistry is both science and art. The science of medicinal chemistry offers mankind one of its best hopes for improving the quality of life. The art of medicinal chemistry continues to challenge its practitioners with the need for both intuition and experience to discover new drugs. Hence sharing the experience of drug research is uniquely beneficial to the field of medicinal chemistry. Drug research requires interdisciplinary team-work at the interface between chemistry, biology and medicine. Therefore, the topic-related series Topics in Medicinal Chemistry covers all relevant aspects of drug research, e.g. pathobiochemistry of diseases, identification and validation of (emerging) drug targets, structural biology, drugability of targets, drug design approaches, chemogenomics, synthetic chemistry including combinatorial methods, bioorganic chemistry, natural compounds, high-throughput screening, pharmacological in vitro and in vivo investigations, drug-receptor interactions on the molecular level, structure-activity relationships, drug absorption, distribution, metabolism, elimination, toxicology and pharmacogenomics. In general, special volumes are edited by well known guest editors. , This work is based on the research supported by the South African Research Chairs Initiative of the Department of Science and Technology and National Research Foundation of South Africa (Grant No 98566), the Cancer Association of South Africa (CANSA), Medical Research Council South Africa (MRC-SA) and Rhodes University. The views expressed are those of the authors and should not be attributed to the DST, NRF, CANSA, MRC-SA or Rhodes University. We apologize if we have inadvertently missed any important contributions to the field.
- Full Text: false
- Date Issued: 2016
- Authors: McAlpine, Shelli R , Edkins, Adrienne L
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66359 , vital:28940 , https://doi.org/10.1007/978-3-319-32607-8
- Description: publisher version , Introduction: Medicinal chemistry is both science and art. The science of medicinal chemistry offers mankind one of its best hopes for improving the quality of life. The art of medicinal chemistry continues to challenge its practitioners with the need for both intuition and experience to discover new drugs. Hence sharing the experience of drug research is uniquely beneficial to the field of medicinal chemistry. Drug research requires interdisciplinary team-work at the interface between chemistry, biology and medicine. Therefore, the topic-related series Topics in Medicinal Chemistry covers all relevant aspects of drug research, e.g. pathobiochemistry of diseases, identification and validation of (emerging) drug targets, structural biology, drugability of targets, drug design approaches, chemogenomics, synthetic chemistry including combinatorial methods, bioorganic chemistry, natural compounds, high-throughput screening, pharmacological in vitro and in vivo investigations, drug-receptor interactions on the molecular level, structure-activity relationships, drug absorption, distribution, metabolism, elimination, toxicology and pharmacogenomics. In general, special volumes are edited by well known guest editors. , This work is based on the research supported by the South African Research Chairs Initiative of the Department of Science and Technology and National Research Foundation of South Africa (Grant No 98566), the Cancer Association of South Africa (CANSA), Medical Research Council South Africa (MRC-SA) and Rhodes University. The views expressed are those of the authors and should not be attributed to the DST, NRF, CANSA, MRC-SA or Rhodes University. We apologize if we have inadvertently missed any important contributions to the field.
- Full Text: false
- Date Issued: 2016
South African perceptions of the good life twenty years into democracy
- Authors: Moller, Valerie
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/67215 , vital:29060 , https://doi.org/10.1007/978-3-319-20568-7_15
- Description: publisher version , South Africa celebrated 20 years of democracy in 2014. In 1994, life satisfaction among all South Africans peaked following the first open non-racial elections. Since that time, only some 45–55 % of the total population, on average, state that they are satisfied. Drawing on Alex Michalos’ classic Multiple Discrepancy Theory (MDT), this chapter explores the needs, expectations, aspirations and perceptions of progress among black South Africans, who were promised a better life under democracy by the new government they voted for in 1994. Findings suggest that expectations raised by the new government in the early years of democracy, coupled with a strong sense of entitlement to state services and welfare in later years, are among the strongest drivers of life satisfaction 20 years into democracy. South Africa’s democracy project is still a work in progress and black South Africans continue to hope for a better life in the future.
- Full Text: false
- Date Issued: 2016
- Authors: Moller, Valerie
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/67215 , vital:29060 , https://doi.org/10.1007/978-3-319-20568-7_15
- Description: publisher version , South Africa celebrated 20 years of democracy in 2014. In 1994, life satisfaction among all South Africans peaked following the first open non-racial elections. Since that time, only some 45–55 % of the total population, on average, state that they are satisfied. Drawing on Alex Michalos’ classic Multiple Discrepancy Theory (MDT), this chapter explores the needs, expectations, aspirations and perceptions of progress among black South Africans, who were promised a better life under democracy by the new government they voted for in 1994. Findings suggest that expectations raised by the new government in the early years of democracy, coupled with a strong sense of entitlement to state services and welfare in later years, are among the strongest drivers of life satisfaction 20 years into democracy. South Africa’s democracy project is still a work in progress and black South Africans continue to hope for a better life in the future.
- Full Text: false
- Date Issued: 2016
The National Wellbeing Index in the isiXhosa translation: focus group discussions on how South Africans view the quality of their society
- Moller, Valerie, Roberts, Benjamin J, Zani, Dalindyebo
- Authors: Moller, Valerie , Roberts, Benjamin J , Zani, Dalindyebo
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/67135 , vital:29035 , https://doi.org/10.1007/s11205-016-1481-4
- Description: publisher version , The International Wellbeing Index covers two complementary measures, the Personal Wellbeing Index (PWI) and the National Wellbeing Index (NWI). The focus group study reported here tested the understanding of the NWI when translated into isiXhosa, a language spoken by 6 million South Africans, or 16% of the country’s population. A challenge for the NWI in measuring national well-being is the tendency for meaning to get ‘lost in translation’ in the wording of the instrument, owing to the disparities that exist between levels of living in developed and developing nations. The focussed discussions with native isiXhosa speakers conveyed the different shades of meaning associated with the six domains that make up the NWI. The isiXhosa keywords for the domains of social conditions, the natural environment, national security, and management of the country’s affairs (government) were readily understood, but discussants asked for further clarification of keywords for the domains relating to the economy and business. Conversations showed up the close link between personal and national well-being: discussants drew upon their personal and parochial life experiences along with their knowledge of current affairs to evaluate the nation’s quality of life. They described the social contract between citizens and their government to create a ‘caring society’ that promotes well-being across key domains of national life. Many of the reference standards used to evaluate national well-being were ones postulated to influence personal well-being (Michalos A.C, Social Indicators Research 16(4): 347–413 1985 ). The study also pointed to a potential problem for longitudinal studies if the bipolar satisfaction scale, formerly used to measure the International Wellbeing Index’s PWI and NWI, is changed to a unipolar one. Findings from this pilot study confirm the potential of the NWI as a tool for measuring national well-being cross-culturally.
- Full Text: false
- Date Issued: 2018
- Authors: Moller, Valerie , Roberts, Benjamin J , Zani, Dalindyebo
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/67135 , vital:29035 , https://doi.org/10.1007/s11205-016-1481-4
- Description: publisher version , The International Wellbeing Index covers two complementary measures, the Personal Wellbeing Index (PWI) and the National Wellbeing Index (NWI). The focus group study reported here tested the understanding of the NWI when translated into isiXhosa, a language spoken by 6 million South Africans, or 16% of the country’s population. A challenge for the NWI in measuring national well-being is the tendency for meaning to get ‘lost in translation’ in the wording of the instrument, owing to the disparities that exist between levels of living in developed and developing nations. The focussed discussions with native isiXhosa speakers conveyed the different shades of meaning associated with the six domains that make up the NWI. The isiXhosa keywords for the domains of social conditions, the natural environment, national security, and management of the country’s affairs (government) were readily understood, but discussants asked for further clarification of keywords for the domains relating to the economy and business. Conversations showed up the close link between personal and national well-being: discussants drew upon their personal and parochial life experiences along with their knowledge of current affairs to evaluate the nation’s quality of life. They described the social contract between citizens and their government to create a ‘caring society’ that promotes well-being across key domains of national life. Many of the reference standards used to evaluate national well-being were ones postulated to influence personal well-being (Michalos A.C, Social Indicators Research 16(4): 347–413 1985 ). The study also pointed to a potential problem for longitudinal studies if the bipolar satisfaction scale, formerly used to measure the International Wellbeing Index’s PWI and NWI, is changed to a unipolar one. Findings from this pilot study confirm the potential of the NWI as a tool for measuring national well-being cross-culturally.
- Full Text: false
- Date Issued: 2018
Mr William Gwali Church Moderator and Royal Advisor Mrs Pama
- Authors: Mr Gwali and Mrs Pama
- Date: 2016
- Subjects: UNCATALOGUED
- Type: Image
- Identifier: http://hdl.handle.net/10962/220089 , vital:48591 , KHP-198
- Description: After Church Service Interview of Church Commitee , Use of this resource is governed by the terms and conditions of the Creative Commons "Attribution-NonCommercial-ShareAlike" License (http://creativecommons.org/licenses/by-nc-sa/2.0/) (NEVER CAHNGE THIS FIELD)
- Full Text: false
- Date Issued: 2016
- Authors: Mr Gwali and Mrs Pama
- Date: 2016
- Subjects: UNCATALOGUED
- Type: Image
- Identifier: http://hdl.handle.net/10962/220089 , vital:48591 , KHP-198
- Description: After Church Service Interview of Church Commitee , Use of this resource is governed by the terms and conditions of the Creative Commons "Attribution-NonCommercial-ShareAlike" License (http://creativecommons.org/licenses/by-nc-sa/2.0/) (NEVER CAHNGE THIS FIELD)
- Full Text: false
- Date Issued: 2016